Operating instructions - Cary 100 Bio UV
Contents
1. 2 5 Access the Setup controls using the Setup button at left and set the following parameters to suit your experiment Cary Uptions Baseline Accessores Accessores 2 Accessones 3 Samplers Reports Auto Store Cary Instrument Control t Mode Cycle Cycle mode Scan Controls Ave time 2 0 100 Data interval nm 1 000 Scan rate nm min 600 000 Temperature Monitor Show Status Display 2 5 1 Under the Cary tab 2 5 1 1 X Mode sets the scan range to be marked in nanometers wave numbers or angstroms 2 5 1 2 X Start and Stop sets the region of the spectrum to be scanned 375 to 700 nm is a typical range using a plastic cuvette 2d 2 5 1 4 Y Mode sets the output data to be reported as absorbance transmittance or other parameters 2 5 1 5 Y min and max sets the upper and lower bounds on the graph the Cary system will use to display the collected data 2 5 1 6 Cycle parameters instructs the Cary system to take multiple scans of the same sample at a specific time separation 2 5 1 7 Scan controls note these parameters are interdependent 2 5 1 7 1 Ave time controls the length time the Cary will collect a signal for each data point in the X range 2 5 1 7 2 Data interval sets the distance between data points in the X range 2 5 1 7 3 Scan rate sets how quickly the Cary will scan the X range Cary Options Base2. Help oll 0 0207 apg VARIAN 2 be Nw Peel 8 Ale OS kt Bk m Ho graph selected Clear report Recalculate Idle Inserta blank sample into the sample compartment and press the OE button to collectthe 1U0 T baseline scan Block the sample beam and press OF to collect the Uts T baseline scan Cancel Block sample beam e g floppy disk B Scan Online Kef File Edit View Commands Setup Graph Maths i 0 003 ot VARIAN jae x Feo a AT BS ket ak m 400 500 600 Wavelength nm Graph 1 Baseline 0 T Remove then click zero 2 7 Under the Accessories 1 tab Cay Options Baseline Accessories 1 Accessories 2 Accessones 3 Samplers Reports Auto Store Accessory Setup and Control Cells Temperature Use Cell Changer Automatic Temperature Setting Select Cells Temperature Controller oto Cell 127 Peltier m S Temperature Display Block Probes 1 J2 ABA Use ABA Show Status Display 2 7 1 Use cell changer enables or disables the automated motor for testing multiple solutions in a single run these instructions assume you are using the cell changer 2 7 2 Select cells allows you to select which cells the spectrophotometer will scan select any cells 1 6 which will contain a sample during your experiment n b selecting any cell 7 14 will force the spectrophotometer into single beam mode
3. 2 8 Under the Reports tab 2 8 1 Save data as ascii e mail to yourself for use in later plotting in Excel or equivalent program 3 Data Collection Prior to data collection clear all traces Setup experiment and scanning range 375 800 nm EM Scan Online fe fe ix File Edit Yiew Commands Setup Graph Maths Help zero baseline 0 0109 n sarn 2v 800 00 sep JSR y Fol a AG Beto AA m File Edit View Commands Setup Graph Maths Help zero baseline ay 0 5629 7 493 33 Abs VARIAN Ati sae E 600 Wavelength nm Report Time Fri 01l Oct 08 40 02 AM 2010 A Method Batch C Varian Cary Winuv inorg Lufaso Instrument Test DSW Software version 3 00 303 Operator Scan Analysis Report Report Time Fri 0l Oct 08 49 28 AM 2010 Method Batch C Varian Cary Winuv inorg Lufaso Instrument Test DSW Software version 3 00 303 Operator Scan Analysis Report Report Time Fri 0l Oct 08 49 51 AM 2010 Method Batch C Varian Cary Winuv inorg Lufaso Instrument Test DSW Software version 3 00 303 a Operator Changing filter Finish collecting and note wavelength of maximum absorbance and absorbance in your notebook Print the report If needed save the data as a Spreadsheet ACSI file for later retrieval and plotting in Excel File name Files of type Spreadsheet Ascii CSW 0 m Ascii Coy Sy e Save only focussed trace 3 1 If needed create batch file for
4. Operating instructions Cary 100 Bio UV visible Spectrophotometer This document describes how to power up the spectrophotometer set its measurement parameters insert one or more of samples for analysis and collect data Software configuration 1 1 Turn on the spectrophotometer using the power switch located directly beneath the Cary 100 Bio logo and log into the computer terminal with your N number 1 2 Open the Cary WinUV folder on the computer s desktop and start launch the Scan program The Varian scan software should launch Before proceeding wait one minute for the software to finish establishing its connection to the spectrophotometer Physical Preparations 2 1 Obtain two clean cuvettes If desired mark one non transmitting face of each as the front when placing them in the instrument orient those faces towards the beam Rinse them with the solvent you used to prepare your solutions 2 2 Fill each cuvette to at least 34 capacity one with only the solvent this is your blank the other will contain a solvent to first obtain the baseline and then the solution of your sample 2 3 Wipe the cuvettes transmitting sides with a Kimwipe while holding the top of the cuvette 2 4 Use 8x6 cell changer if needed and this assumes double mode norms Scan Online fm X File Edit View Commands Setup Graph Maths Help ad 0 0282 ans VARIAN Ho graph selected Clear report Recalculate
5. line Accessories 1 Accessories 2 Accessories 3 Samplers Reporte Auto Store Advanced Settings SBME nergy Signal to H ose Signal to noise mode SBW rm Beam mode Double a Source Display Options Auto lamps off Individual data Overlay data Source changeover nm 350 00 2 5 2 Under the Options tab 2 5 2 1 Spectral bandwidth the width in nm of the light at half peak height can be used to increase the signal to noise ratio or the spectral resolution of the instrument 2 5 2 2 Beam mode Double is recommended as it will scan the sample and the reference solution simultaneously to generate a spectrum that is independent of the solvent and cuvette s aborptivity these instructions assume you are using Double mode 2 6 Under the Baseline tab Cam Optiong Baseline Accessories 1 Accessories 2 Accessories 3 Samplers Reports Auto Store Baseline Selection Correction None Retrieve Baseline file Baseline conection View Baseline file Zero baseline correction Zero SRA correction O Zero std ref correction O Known mirror 2 6 1 Execute a Zero baseline correction with DI water in cell holder 1 and 9 Press OK then baseline Scan Online DER Fie Edit wiew Commands Setup Graph Maths
6. your data Go to the File dropdown menu then Save Data As then use the Look in dropdown menu to open the My Computer C Documents and Settings directory Open the folder with your N number as its title and then open its Desktop subfolder Give your batch file a descriptive filename and click Save
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