User Guide Sentino™ Microbiology Pump
Contents
1. Life Sciences User Guide Sentino Microbiology Pump Product Numbers 13180 13181 For laboratory use Not for use in a manner other than indicated A d E 4 DW 1 AN 65 al Filtration Separation Solution sm Table of Contents LaLe SU COM RTT ates 3 Bore qeu Ly Cedi Le DNO Ete 3 Product WOM pla iecore c e m 4 Symbols aiii Ee ac ERR ROO 5 Salety mducie rs UAR EIN cased aesscesacxicsanndaceascnscinesdosatsnsacedasasseensechessiseanastansancdesaveascedeanssaces eescaxecacdacacaseosasescnera 5 6 Ads arie cipe ROT I ERE E Eo A QU t re re ee ee ee eee ere 6 Preparo Systemi Tor USE Te ORCI 7 PU clie p ENTERO PN 8 Bet A E B A AEE EEEN nd AE ueri E EEEN REY VAAN E RD a E IIA ONA AENEAN I CO MR NM M dM 8 9 Rie leccgeenioeR M S 9 19 M T 10 e cine DE ne en Rn T Tec TRY 10 Disposal BR cR TENTE ttm 10 EC Declaration of Conformity t m 11 2 Life Sciences Introduction Application and Intended Use The Membrane Filter MF Technique is a method of analyzing aqueous samples for microbial contamination A fluid sample is pulled through a membrane filter and the filter is placed on growth media and incubated After the prescribed incubation period colonies are counted and reviewed for appropri2. using power transformer plugged into main and unit not operating power source until battery is fully charged Pump will not turn on Battery is discharged Recharge battery If problem is not corrected call for service no yellow fault indicator Pump head not rotating Obstruction in pump head Check that drive tube slot in pump head is free of foreign objects yellow fault indicator illuminated Verify Fluid Path is properly loaded See Preparing System for Use Fluid Path Installation Loading Pump over temperature limit Check the temperature of the operating environment and allow pump to cool Pump head rotating but fluid Pump head loading door is open Verify Fluid Path is properly loaded and loading door is securely is not filtering closed See Preparing System for Use Fluid Path Installation Loading Fluid too viscous Consider whether sample can be diluted with sterile buffer to facilitate filtration Fluid too particulate laden Consider whether MF Technique is the appropriate method for microbial evaluation for the heavy particulate load or process sample in multiple aliquots to facilitate filtration If the above corrective actions do not remedy the problem contact manufacturer for service or warranty www pall com lab 9 Service o Save original packaging for service and warranty repairs Pall does not recommend any service be performed on this device other than by the manufacturer Contact Pall Life Sciences for technical sup
3. ate morphology Pall Life Sciences Sentino Microbiology Pump is ideal for microbiological evaluations of aqueous samples by MF Technique This simple compact unit will optimize and maximize your workspace environment by eliminating the need for separate manifold pump and excess tubing The Sentino Pump is designed to use peristaltic action to draw the sample through the filter and send filtrate directly to drain or waste collection Operating parameters are preset and fixed to best satisfy requirements for MF Technique described in EPA ISO and ASTM methods thereby providing conditions comparable to a standard manifold set up Ordering Information Sentino Microbiology Pump Part Number 13180 Description system for North America 1 power transformer 1 power cord with NEMA 5 15P plug 1 Fluid Path 1 Pkg 1 pkg 13181 Accessories Part Number 4861 1 User Guide CD S ystem for Locations Outside North America 1 power transformer 1 European power cord with CEE 7 7 plug 1 UK power cord with BS1363 plug 1 Fluid Path 1 User Guide CD Description Fluid Path disposable for use with MicroFunnel filter funnels 1 pkg Pkg 10 pkg www pall com lab Product Terminology Keypad 1 Power On Off button 2 Main power indicator 3 Pulse button 4 Fault indicator 5 Battery status indicator Body 6 Stage with adapter guide 7 Drive tube guide 8 Product identification label 9 Pow
4. er entry socket Pump Head 10 Loading door 11 Slot for drive tube Power 12 Transformer power entry plug 13 Transformer input interface 14 Power cord Fluid Path 15 Filter funnel adapter 16 Drive tube 4 Life Sciences Symbols Referenced A See manual for instructions Pinch point C C The Sentino Microbiology Pump a product of Pall Corporation is in conformity with the requirements of the European Directive 2004 108 EC and associated amendments with regards to Electromagnetic Compatibility and the European Directive 2006 42 EC and associated amendments with regards to Machinery See EC Declaration of Conformity section this label on a product means that the product contains electrical or electronic materials and there fore it should not be disposed of as unsorted waste but instead be treated separately The presence of these materials may if not disposed of properly have potential adverse effects on the environment and human health Within the European Union users are urged to recycle such products when being replaced with a newer version or when they have outlived their useful lives However as the legislation and facilities vary throughout the member states please visit www pall com weee to arrange for proper disposal of used equipment Reuse recycling and recovery The materials used in this unit are ROHS compliant The presence of Safety Precautions To protect personal safety and ensure cor
5. hange without notice For current information consult your local Pall distributor or contact Pall directly 2011 Pall Corporation Pall atb MicroFunnel and Sentino are trademarks of Pall Corporation indicates a trademark registered in the USA Filtration Separation Solution is a service mark of Pall Corporation 7 11 PDF GN10 4652 PN89724B
6. ign Part 1 Basic Terminology methodology EN 12100 2 2003 Safety of Machinery Basic Concepts General Principles for Design Part 2 Technical principles and specifications A risk assessment has been performed and documented in accordance with EN 14121 1 2007 Safety of Machinery Principles for Risk Assessment Signed LO lamt Date March 25 2011 Carole Durant Vice President Quality Assurance and Regulatory Affairs For and on behalf of Pall Europe Limited www pall com lab 11 Life Sciences Pall Life Sciences 600 South Wagner Road Ann Arbor MI 48103 9019 USA 1 800 521 1520 USA and Canada 800 PALL LIFE Outside USA and Canada 1 734 665 0651 phone 1 734 913 6114 fax Filtration Separation Solution Visit us on the Web at www pall com lab E mail us at LabSupport pall com International Offices Pall Corporation has offices and plants throughout the world in Argentina Australia Austria Belgium Brazil Canada China France Germany India Indonesia Ireland Italy Japan Korea Malaysia New Zealand Norway Philippines Poland Russia Singapore South Africa Spain Sweden Switzerland Taiwan Thailand United Kingdom and Vietnam Distributors in all major industrial areas of the world To locate the Pall office or distributor nearest you visit www pall com contact The information provided in this literature was reviewed for accuracy at the time of publication Product data may be subject to c
7. nto filter funnel On Off operation i 3a To start filtration press and release the power On Off button 3b To stop filtration press and release the power On Off button again OR Pulse or momentary operation 3a Press and hold the pulse button IL to perform momentary filtration 3b Release the pulse button JT Lto stop filtration 4 Perform rinses in the same manner as sample filtration Maintenance A Do not immerse A The device is designed for use with Pall s Fluid Path only A The unit is always on standby with battery power Avoid pressing power or pulse buttons while cleaning pump head Use of tubing not recommended by Pall Corporation may cause damage to pumping mechanism reduce performance or cause tube breakage This will void all manufacturer warranties The device utilizes a peristaltic pump drive which may cause a pinch point if operated with the loading door open Visit www pall com sentino for a video on system preparation and operation 8 Life Sciences Cleaning Body and Pump Head e May be wiped down with a towel moderately soaked in the following common laboratory sanitizing solutions o 70 isopropyl alcohol IPA o 10 bleach Sanitizing the Fluid Path e he Fluid Path should be changed at least once a week e For optimal contamination control daily replacement of Fluid Path is recommended e Upon initial installation of the Fluid Path o Run 7096 IPA through the Fluid Path fo
8. ower or pulse buttons while loading Fluid Path Use of tubing not recommended by Pall Corporation may cause damage to pumping mechanism reduce performance or cause tube breakage This will void all manufacturer warranties G3 The device utilizes a peristaltic pump drive which may cause a pinch point if operated with the loading door open Loading 1 With unit positioned on benchtop open pump loading door fully 2 Slide adapter into the guide on the pump housing until it is firmly seated 3 Tuck the tubing into the slot in the pump head and position the tube into the guide slot in the base 4 Remove tubing slack by firmly pulling the tubing against the rollers as you close the pump loading door to a snap closed position 5 Sanitize Fluid Path before use See Pump Operation NOTE O If pump head fails to rotate when turned on then turn off the pump open the pump head door and repeat Step 4 www pall com lab T Pump Operation Operation is the same whether powered by the internal battery or using the power transformer and power cord o If the loading door is open during operation no filtration or pumping will occur O Visit www pall com sentino for a video on system preparation and operation If the loading door is open during operation rotation of the rollers may present an accessible pinch point to the user Filtration 1 Place Pall filter funnel onto adapter on pump stage 2 Pour sample i
9. port warranty and service inquiries at 800 521 1520 inside USA and Canada 800 PALL LIFE outside USA and Canada or LabSupport pall com Specifications Materials of Construction Housing Material Polycarbonate ABS blended polymer Keypad and Label Polyester Pump Head PEI with PTFE filled PBT rollers Fluid Path Adapter Polypropylene Hose Material Thermoplastic elastomer Dimensions Length 13 6 cm 5 4 in Width 9 5 cm 3 7 in Height 10 4 cm 4 1 in Weight 680 g 1 5 Ibs Hose Dimensions 4 8 mm 3 16 in ID x 7 9 mm 5 16 in OD 1 6 mm 1 16 in wall thickness Temperature Range Operation 15 30 C 59 86 F Storage 15 45 C 5 113 F Fluid Path should be allowed to equilibrate to operation range before use Battery Voltage 12 0 V Type NiMH nickel metal hydride DC Supply Voltage 24 0 V AC DC Power Transformer 24 VDC 1 6 Amp 100 240 VAC 47 63 Hz Input Interface IEC 320 3 pin socket Output Interface Power Entry Plug 2 5 mm barrel jack Power Cord 13180 IEC 320 connector and NEMA 5 15P grounded plug 13181 IEC 320 connector and European CEE 7 7 plug IEC 320 connector and UK BS1363 plug Disposal Instructions this label on a product means that the product contains electrical or electronic materials and therefore it should not be disposed of as unsorted waste but instead be treated separately The presence of these materials may if not disposed of properly have po
10. r 5 seconds o Pump residual IPA through the tube e f the Fluid Path is used for more than one day o At the end of the filtration session run 70 IPA through the Fluid Path for 5 seconds o Pump residual IPA through the tube o Open the pump head loading door to relieve the pinch of the rollers on the drive tube Troubleshooting This symbol on the unit is backed with a yellow LED and will illuminate to indicate the device has sensed A a condition in which performance has been impacted and the user should consult User Guide for troubleshooting Problem Cause Corrective Action Plugged into main power source Incorrect power transformer Use power transformer supplied with pump but green main power indicator Incorrect power source Check that power source supplies 100 240 VAC not illuminated Pump plugged into main power Obstruction in pump head Check that drive tube slot in pump head is free of foreign objects but green main power indicator Verify Fluid Path is properly loaded not illuminated and yellow fault See Preparing System for Use Fluid Path Installation Loading ue A alan Pump over temperature limit Check the temperature of the operating environment and allow pump to cool Pump slowing down with or without Battery discharging Continue filtration using power transformer plugged into main yellow fault indicator flashing power source until battery is fully charged Yellow fault indicator flashing Battery is discharged Continue filtration
11. rect use of this equipment be sure you read and understand this guide before use The following terms are used DANGER To designate a condition which MAY involve mortal danger or serious injury CAUTION A To designate a condition which MAY involve serious injury or destruction of the equipment involved NOTE o To designate a condition which MAY involve faulty operation of the equipment thus causing poor performance Universal Precautions Appropriate care should always be taken when using electrical equipment around aqueous solutions in the laboratory Use of personal protective equipment such as gloves gowns laboratory coats and protective eye wear help to reduce risk of exposure and is recommended Sanitization of equipment as directed in this manual Is essential for maintaining optimum performance Proper disposal of Fluid Paths in accordance with biohazard safety procedures is recommended where applicable T Opening or disassembly of product enclosure may involve electrical shock Only authorized personnel should disassemble product 1 Do not immerse 4 Only use parts approved by the manufacturer www pall com lab For laboratory use only Keypad buttons on this product intended for finger touch only Only use recommended solutions for cleaning sanitization The unit is always on standby with battery power Avoid pressing power or pulse buttons while cleaning pump head or loading Fluid Path Prolonged use in en
12. tential adverse effects on the environment and human health Within the European Union users are urged to recycle such products when being replaced with a newer version or when they have outlived their useful lives However as the legislation and facilities vary throughout the member states please visit www pall com weee to arrange for proper disposal of used equipment 10 Life Sciences Reuse recycling and recovery The materials used in this unit are ROHS compliant The presence of foes Pall Corporation EC Declaration of Conformity CE Product Description Sentino Microbiology Pump Product Part Number s 13180 and 13181 The Sentino Microbiology Pump a product of Pall Corporation is in conformity with the requirements of the European Directive 2004 108 EC and associated amendments with regards to Electromagnetic Compatibility and the European Directive 2006 42 EC and associated amendments with regards to Machinery This declaration is based upon testing the compliance of the product against the limits of the following standards EN 61326 1 2006 Electrical Equipment for Measurement Control and Laboratory Use EMC Requirements Part 1 General Requirements EN 60204 1 2006 Safety of Machinery Electrical Equipment of Machines Part 1 General Requirements and assessing the compliance of the product to these harmonized standards EN 12100 1 2003 Safety of Machinery Basic Concepts General Principles for Des
13. vironments exceeding 30 C 86 F may cause overheating The device utilizes a peristaltic pump drive which may cause a pinch point if operated with the loading door open Return product in original packaging for warranty repair Warranty valid only when used in accordance with this User Guide A A A A A O Keypad and Symbols MAIN POWER INDICATOR A green LED will illuminate when the pump is plugged into the power transformer and VAC power e source This LED will not be illuminated when operating on battery power POWER ON OFF 5 Press the Power On Off button to turn on the pump Press the Power On Off a second time to turn off the pump PULSE OPERATION n The pump will operate only while the button is pressed This feature is ideal for small fluid volumes that require momentary filtration intervals FAULT INDICATOR N This symbol will illuminate yellow upon detection of a fault in the system operation Flashing indicates battery is running low and solid illumination indicates other faults detected Consult the Troubleshooting section of this User Guide BATTERY INDICATOR C7 When plugged into the power transformer and VAC power source this symbol will indicate the battery is charging by an illuminated blue LED When the battery is fully charged the blue LED will turn off 6 Life Sciences Preparing System for Use Save original packaging for service and warranty repairs Visit www pall com sentino for a video O on s
14. ystem preparation and operation Device Set Up Connecting power supply and charging 1 Position the Sentino Microbiology Pump on a laboratory bench 2 Attach power entry plug from power transformer into the power socket Connect power cord to the input interface on the power transformer then plug power cord into appropriate electrical outlet having 100 240 VAC supply 3 Confirm Main Power Indicator is illuminated Battery Status Indicator should be illuminated blue to indicate battery is charging 4 The pump can be used while on the power transformer as battery is charging 5 Upon initial use or when device has been in storage for a long period of time completely charge battery typically 4 5 hours When not in use pump should remain connected to main power to ensure full battery charge is available at time of use f If the battery has been fully discharged the pump will turn on when initially connected to power source Press power On Off button to turn off pump and continue to use or charge Fluid Path Installation The Sentino Microbiology Pump is designed to interface with Pall s disposable Fluid Path The Fluid Path will remain secure during filter funnel loading and unloading A guide on the pump housing helps position the tubing correctly inside the device without the use of any tools A The device is designed for use with Pall s Fluid Path only 4 Ihe unit is always on standby with battery power Avoid pressing p
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