User Guide
Contents
1. Getting to Know Your Bio 6000 The Bio 6000 is a professional gel scanner specially designed and optimized for image capture of biological samples from gels or transparent film It features a 12 x 16 9 scan bed 48 bit color depth 3200 x 6400 dpi optical resolution and one Smart Touch button on the front panel for an easy access to the scan function With a Dmin of 0 05D and a Dmax of 3 77D the Bio 6000 can deliver a wide dynamic range to enhance the image quality in both bright and dark areas of the image Additionally the Bio 6000 also lets you scan reflectives and prints up to 12 x 16 9 in size convenient for any biological laboratory use Contents Getting to now Your Bio 6000 cc eei 1 Positioning Electrophoresis Gels Film or Transparent Film 10 Features ofthe Blo DDO0 s concerto seme occ cete oec oto ae E Ds 2 Positioning Reflective Materials en enem eee ti on tet eux ase her nta necne Ya nu 10 Taking aGhoser book n MER cp MED nmr clenimdurvanrentornamerstennecenetnaerinar 3 ThedislackBlate s coe ERU EN ere Ti Initial Setup etes et IU EM Ae YOUNIS Stal cee cc ce om ee Le M Rd 12 Step 1 Unpacking Package Contents errore eren nennen nennen ansa nenas Ag Scadniid SCOMAMOS deese EE EE 14 Step Installing the SOMWane maen e e e E EE 5 Scanning Electrophoresis Gels or Film eseseeeeeee 15 Steps Unlockingthe Scale eec IURE 6 Scanning Reflective Materials v ecco trece eerte2. 627 mmx375 mm x 185 mm 35 4 lbs 16 1 kg AC 100V 240V 47 63 Hz 1 5 A max 54 0 W max Operating Temperature 50 F to 104 F 10 C to 40 C Relative Humidity 2096 to 8596 System Requirements General Requirements CD ROM drive for installing software e Color display with 24 bit color output capability e 512 MB RAM or more PC and compatibles e Pentium IV PC or higher with Hi Speed USB USB 2 0 port Microsoft Windows 7 compatible with Windows XP Vista and Windows 8 Important Specifications software bundles and accessories are subject to change without notice Not responsible for typographic errors Bio 6000 User Guide 19 FCC Compliance Statement This equipment Model MRS 6400A3PL has been tested and found to comply with the limits for a Class B digital device pursuant to Part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference in a residential installation This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interf
3. Click the Overview button to perform a preliminary scan of the image which will appear in the Preview window Select the Scan Frame tool from the Toolbar in the Preview window and choose the area to be scanned by dragging a rectangle around it You will see a flashing frame marquee around the selected area Click the Prescan button to display a detailed image area selected via the Scan Frame tool A thumbnail of the image appears as well in the Scan Job Queue window Specify your scanning requirements in the Settings window a Select a desired image type b Select a desired resolution c Adjust the scan frame settings if necessary Adjust image quality if necessary using the Advanced Image Correction AIC tools Click the Scan to or Scan button in the Preview window or on the scanner unit to start scanning e fScanWizard Bio was launched from an application program the image is then delivered to your application where the image can be saved printed or edited e fScanWizard Bio was launched in stand alone mode you will be prompted to specify the file attributes for the scanned image after the Scan or Scan to button is pressed such as entering the file name specifying a folder name of your own etc When you have completed the settings press the Done Save button and the scanner will automatically scan and save your image based on your settings Bio 6000 User Guide 17 Scan Job Untitled v t
4. Type RGB Colors v Resolution 300 Scan Frame x Scaling Output W 8 17 8 17 Es 100 v Ea E H 11 65 11 65 Image Size 25 080 KB M Sampling WitefBlack Ps el Tone Curve No Correction Bu Contras EJ NB Automatic Color Restoration Settings window o Qo 9 w Reflective Transmis sive Fr Bio Microtek ScanWizard Scanner View Preferences Correction Help T TI TIT amp Magnify Pan B DropW Drop ProfessionallGellScannertfor Electrophoresis Gel SDS PAGE W dthrn Blotting etc MICROTEK wro Wi Preview window Status Type Job Title Size 13 630 KB Total Scan Jobs 1 Job s Scanned D Scan Job window While selecting press CTRL key to keep aspect ratio selection or press SHIFT key to add new job MICROTEK c Material 18 Bio 6000 User Guide Specifications Scanning Modes Light Source Scanning Area Resolution Dynamic Range Interface Dimensions L x W x H Weight Voltage Power Consumption Environment Color and grayscale in a single scanning pass True 48 bit color approx 281 billion colors 16 bit grayscale approx 65 536 shades of gray LED Reflective 12 x 16 9 304 8 mm x 429 26 mm Transparent 12 x 16 1 16 304 8 mm x 408 mm Optical 3200 H dpi x 6400 V dpi Transparency 0 05D 3 77D 3 77 Dmax Hi Speed USB USB 2 0 24 7 x 14 8 x 7 3
5. before putting the U shaped Ruler on the scanner glass surface make sure the scanner is turned off To use the U shaped Ruler 1 Peel the protective film from the back side of the ruler before using it to calibrate the scanner 2 Then put the ruler facing up on the scanner glass surface and hook the ruler onto the raised Silicone Rings located on the both sides of the scanner surface making sure that they have been oriented correctly Silicone Rings U shaped Ruler 3 Finally turn on the scanner power Bio 6000 User Guide 9 Positioning Electrophoresis Gels Film or Transparent Film To scan the electrophoresis gels film such as 1D 2D gels SDS PAGE western northern southern blots or membranes and transparent film such as slide positive film or reversal film you have to scan gels or film together with the U shaped Ruler Calibration Ruler e Toscan with U shaped Ruler refer to the Using the U shaped Ruler Gel or film section in this guide to place the U e Calibration shaped Ruler first then place and gt center the gel or film on the scanner glass surface Make sure to keep the area of the calibration strip clear Positioning Reflective Materials To scan reflective materials such as photo prints dissertations papers etc you have to scan the reflective original together with the U shaped Ruler Calibration Ruler and Black Plate Before positioning the reflective make su
6. User Guide 5 Step 3 Unlocking the Scanner Before you operate the scanner you will need to unlock the scanner To unlock the scanner follow the steps below 1 Remove the yellow Unlock sticker from your scanner 2 With the scanner power off turn the scanner on its side and locate the locking screw at the bottom of the locking screw counterclockwise to the unlock position scanner SS 3 Using a screwdriver push and turn the x Unlocked When successfully unlocked the screw will push out a little protruding slightly from the bottom of the scanner Locking the Scanner To lock the scanner for shipping and other purposes follow the steps below 1 Turn off the scanner 2 Turn the scanner back on When the indicators on the front of your scanner stop blinking use a screwdriver and then push and turn the locking screw clockwise to the locked position IC OAM A When the screw has been tightened j this indicates that your scanner is locked Locked 3 Turn off your scanner 6 Bio 6000 User Guide Step 4 Installing the TMA 1 Attach the TMA by sliding the posts at the rear of the TMA into the holes 3 Unlocked 2 Plug the connector of the TMA into the scanner s 15 pin accessory port 3 Unlock the TMA a Remove the yellow Unlock sticker from the TMA a Look for the locking k
7. before you continue further scanning scenarios described in next pages 1 Launch ScanWizard Bio either as a stand alone by clicking on the program icon or by using the File Import or File Acquire command from your image editing program 2 Place the gel or film to be scanned facing down on the scanner glass surface with the top end of it toward the front of the scanner Remember to keep the calibration strip clear U shaped Ruler 3 Gotothe Preview window of ScanWizard Bio and choose Transmissive from the Scan Material menu 4 Clickthe Overview button to perform a preliminary scan of the image which will appear in the Preview window 5 Select the Scan Frame tool from the Toolbar in the Preview window and choose the area to be scanned by dragging a rectangle around it You will see a flashing frame marquee around the selected area 6 Click the Prescan button to display a detailed image of area selected by the Scan Frame tool A thumbnail of the image appears as well in the Scan Job Queue window 7 Specify your scanning requirements in the Settings window a Select a desired image type b Select a desired resolution c Adjust the scan frame settings if necessary 8 Adjust image quality if necessary using the Advanced Image Correction AIC tools 9 Click the Scan to or Scan button in the Preview window or on the scanner unit to start scanning e fScanWizard Bio was launched from an applicati
8. eflective media N OO fF WYN U shaped Ruler Calibration Ruler for calibrating your scanner 4 Bio 6000 User Guide Step 2 Installing the Software Important Do not remove the yellow sticker from your scanner until you are told to do so You must install software before connecting your scanner Always close any open programs and turn off Anti virus utilities before installing software 1 Place the Adobe CD ROM into the CD ROM drive and install the software Skip this step if you have a newer version of Adobe Photoshop Elements already installed on your computer 2 Placethe Microtek software CD ROM into the CD ROM drive then follow the on screen instructions to install the driver and software NOTE If the Microtek Software Installer screen does not come up automatically double click the following in succession My Computer the CD ROM icon then cdsetup exe to start the installer program 3 Restart your computer at the end of all software installation Drivers amp Software Upgrades After you finish the installation of software if you found that the installed drivers and software cannot run your product or your computer system properly later please go to the Microtek Download Service site at ww7 microtek com tw service php to download and install any updates you may require For additional information about Microtek products please visit our website at www microtek com Bio 6000
9. erence by one or more of the following measures e Reorient or relocate the receiving antenna e Increase the separation between the equipment and receiver Connect the equipment into an outlet on a circuit different from that to which the receiver is connected Consult the dealer or an experienced radio TV technician for help NOTE A shielded Hi Speed USB interface cable with ferrite core installed on the scanner connector and must be used with this equipment Caution Changes or modifications not expressly approved by the manufacturer responsible for compliance could void the user s authority to operate the equipment This device complies with Part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation 20 Bio 6000 User Guide
10. erials such as photos or prints 14 Bio 6000 User Guide Scanning Electrophoresis Gels or Film Place the gel or film to be scanned on the scanner glass surface as detailed in the Positioning Electrophoresis Gels Film or Transparent Film section of this guide Launch ScanWizard Bio either as a stand alone by clicking on the program icon or by using the File Import or File Acquire command from your image editing program Goto the Preview window and choose Transmissive from the Scan Material menu Click the Overview button to perform a preliminary scan of your original Select the Scan Frame tool from the Toolbar in the Preview window and choose the area to be scanned by dragging a rectangle around it You will see a flashing frame marquee around the selected area Click the Prescan button to display a detailed image area selected via the Scan Frame tool A thumbnail of the image appears as well in the Scan Job Queue window Specify your scanning requirements in the Settings window a Select a desired image type b Select a desired resolution c Adjust the scan frame settings if necessary Adjust 1mage quality if necessary using the Advanced Image Correction AIC tools Click the Scan to or Scan button in the Preview window or on the scanner unit to start scanning e fScanWizard Bio was launched from an application program the image is then delivered to your applicatio
11. n where the image can be saved printed or edited e fScanWizard Bio was launched in stand alone mode you will be prompted to specify the file attributes for the scanned image after the Scan or Scan to button is pressed such as entering the file name specifying a folder name of your own etc When you have completed the settings press the Done Save button and the scanner will automatically scan and save your image based on your settings Bio 6000 User Guide 15 ee Rees Scan Job Untitled v Scan Frame x Scaling Output wpe ze Image Size 13 530 KB White Black Pts vs Tone Curve Brt Contrast E Exposure Time Q 0 00 4 Settings window 9 Status Type Job Title Size P Untitled1 One pass Scan for Multiple Jobs Multiple Auto crop for EZ Lock Film Holder DANHA DE Total Scan Jobs 1 Job s Scanned 0 Scan Job window 16 Bio 6000 User Guide Scanning Reflective Materials Place the reflective original to be scanned on the scanner glass surface as detailed in the Positioning Reflective Materials section of this guide Black Plate Launch Scan Wizard Bio either as a stand alone by clicking on the program icon or by using the File Import or File Acquire command from your image editing program Goto the Preview window of ScanWizard Bio and choose Reflective from the Scan Material menu
12. nob at the base of the TMA 99 b Turn the locking knob counterclockwise to the unlock position Locking the TMA To lock the TMA for shipping and other purposes follow the steps below 1 Make sure the TMA is connected to the Locked scanner then turn off the scanner if your scanner is on 2 Turn the scanner back on The TMA s i carriage will move to the standby oin s position in a few moments 3 When the indicators on the front of your TMA and scanner stop blinking turn the locking knob clockwise to the 22 D lock position 4 4 Turn off your scanner and disconnect the TMA connector from the scanner The TMA is ready for transport Bio 6000 User Guide 7 Step 5 Connecting the Scanner To the power adapter 1 Connect the power cord to the back of the scanner 2 Plug the power cord into a power source To the Hi Speed USB cable 3 Connect one end of the cable to your computer 4 Connect the other end of the cable to the scanner 5 Press the power switch at the back of your scanner and wait for the indicator light on the front panel to stop blinking and stay on steady The system will detect your scanner automatically 8 Bio 6000 User Guide Using the U shaped Ruler Calibration Ruler The U shaped Ruler Calibration Ruler is designed for the use of the scanner calibration Please take note that
13. on program the image is then delivered to your application where the image can be saved printed or edited 12 Bio 6000 User Guide e fScanWizard Bio was launched in stand alone mode you will be prompted to specify the file attributes for the scanned image after the Scan or Scan to button is pressed such as entering the file name specifying a folder name of your own etc When you have completed the settings press the Done Save button and the scanner will automatically scan and save your image based on your settings peser Be Microtel Scania _ a hie M 8m TEES Type __RGB Colors___ un s erts i meum S mw j Scan Frame x Scaling Output W 7 82 7 82 22 ox v2 e 7 aee NN Image Size 13 630 KB Exposure Time Custom EEEE Br Contrast J Settings window AMICROTEK pru EU Ure I Saye EY Preview window Status Job Title One pass Scan for Multiple Jobs Multiple Auto crop for EZ Lock Film Holder 3 Ge ZO Total Scan Jobs 1 Job s Scanned D Scan Job window Bio 6000 User Guide 13 Scanning Scenarios The following pages provide various scenarios for scanning with the Bio 6000 including the following e Scanning electrophoresis gels or film This scenario details the steps for scanning electrophoresis gels or film e Scanning reflective originals This scenario details the steps for scanning reflective mat
14. re that the U shaped Ruler is on the scanner glass surface properly 1 Place the reflective original to be scanned face down on the scanner Black Plate glass surface with the top end of it toward the front of the scanner 2 Peelthe protective film from the Black Plate 3 Put the Black Plate on the top of the reflective to cover it with the side of the Black Plate that reads This side up facing up 10 Bio 6000 User Guide The Black Plate Light from the TMA and other ambient light during scanning may result in overexposed images The Black Plate is designed to work with reflective originals To ensure correct exposure and optimal image quality during scanning the Black Mat should be used to shield unwanted light out Take note of the following before using the Black Plate 1 The Black Plate may become dirty over time and with prolonged use To clean the plate use a white cotton cloth dipped in some water and gently wipe the plate surface This ensures that the Black Plate is in optimal condition at all times 2 A protective film covers the Black Plate during shipping and prevents it from being scratched When peeling the film from the plate be careful not to tear or bend the Black Plate Bio 6000 User Guide 11 Your First Scan This section is to guide you to perform your first scan by using the ScanWizard Bio You should familiarize yourself with the basic scanning technique
15. tec reo rennes 17 Step 4 Installing te TMA s reo eR E Ide 7 Specifications System RE EME 19 Step 5 Connecting the Scanner s s erssrsrrsrsrrrrrrnerirnrnrenrertnrereererrerennne PEE Fe eompliance staternent es 11s eec onto 20 Using the U Shaped Ruler Calibration Ruler eese 9 Copyright 2013 Microtek International Inc http www microtek com 4 149 004826 D September 2013 MICROT h Features of the Bio 6000 The Bio 6000 comes with several important features including the following Large 12 x 16 9 scan bed The tabloid size scan bed of the Bio 6000 lets you easily scan biological samples from a gel or film up to 12 x 16 1 16 and oversized reflective originals such as contact sheets large pieces of art and mechanical blueprints e 3200x 6400 dpi resolution The exceptionally high resolution of the Bio 6000 lets you scan even postage size images and enlarge them with amazing clarity with little loss of detail The scanner s 3 77 maximum optical density allows it to capture a wide range of tones approximating real life color and hues e Smart Touch button A Smart Touch button Scan locating at the front panel of the scanner provides you a quick and easy way to capture images that can be automatically saved as files or sent to another application for further processing later e Energy saving LED light source Adopting LEDs as the light source when the scanner is detected by the s
16. ystem there are no requirements for any warm up time before carrying out the scan which boots your productivity and reduces energy costs amazingly With its stable performance the image quality will remain consistent even after used for a certain period of time Microtek ScanWizard Bio scanning software This is a scanning software for the use of the electrophoresis gel film scanning Friendly and easy to use interface with powerful functions and controls offer you a quick access and management for all your scanning tasks e Microtek s MiBio software This is a management software developed exclusively for Bio tech analysis and research It features several easy to use but very practical functions supporting users with powerful tools in a clear and simple control panel 2 Bio 6000 User Guide Taking a Closer Look TMA Scanner Glass surface Scan bed Vertical ruler READY indicator Green POWER indicator Orange Smart Touch button Scan Accessory port or with TMA s connector securely connected Power connector Hi Speed USB port 1 Power switch Bio 6000 User Guide 3 Initial Setup Step 1 Unpacking Package Contents After unpacking your scanner package please check for the major components listed below Bio 6000 lower base USB Cable Power Cord Software CDs TMA Transparent Media Adapter Black Plate for scanning r
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