A59443AA: User's Manual, pHI 400 Series and
Contents
1. Description fe wren TT Tf fe ec eee po momo Pf n The pH Conductivity TDS Salinity or DO value is automatically temperature compensated The meter is in a calibration mode Calibrating number indicates the current point of a multiple point calibration The Temperature value is in C or F E E E eieieioall m eee S ene Sere ajem 2 ema as meee nipe 13 14 SECTION 4 Keypad Functions Model pHi 470 Handheld and pHi 570 Benchtop shown Table 3 Keypad Functions Key pHi pHi pHi pHi 410 420 430 450 pHi pHi pHi pHi 510 520 530 550 ON OFF Turns on off meter pa on pnas pn E ER ERA BES E a TDS Displays TDS If no Conductivity probe is attached TDS mode is inactive pHi 460 pHi 560 pHi 470 pHi Displays Conductivity If no conductivity probe is attached conductivity mode is inactive Displays Salinity If no conductivity probe is attached salinity mode is inactive In DO mode with a Salinity probe attached DO is automatically compensated for salinity Puts the meter in the calibration mode for the currently displayed parameter SETUP Enters setup mode CANCEL Cancels an action Cancels an action action Praa aaa barometric pressure TIME Displays the time pressing again displays date STORE Stores a Stores a reading RECALL _ a Recalls a reading DELETE Deletes a stored Deletes a stored reading2. Nominal Value 25 C 0 C 5 C 10 C 20 C 30 C 40 C 50 C 168 1 67 1 67 1 67 1 67 1 68 1 69 1 71 4 01 4 00 4 00 4 00 4 00 401 403 4 06 6 86 6 98 6 95 6 92 6 87 6 85 6 84 6 83 7 00 7 12 7 09 7 06 7 01 6 99 6 97 6 97 9 18 9 46 9 40 9 33 9 23 9 14 9 07 9 02 10 01 10 32 10 25 10 18 10 06 9 97 9 89 9 83 12 45 13 42 13 21 13 00 12 63 12 29 12 04 11 70 20 SECTION 7 Probes and Connections Handheld Meters 3 3 5mm phono jack for pH or 2 BNC Connector for ISE temperature probe or for 3 pH electrode or ISE in 1 electrode probe connector l 7 N lt a gt 4 Dissolved L a bo Sy Oxygen probe ity probe lt i ye connector 5 AC Power Adapter Model pHi 470 Shown other models may not have all the connections shown Probes and Connections Benchtop Models 4 3 5mm phono jack for pH or ISE temperature probe or for 3 in 1 electrode 3 USB Connector 5 AC Power 2 BNC Con nection for pH electrode or ISE probe 6 Dissolved Oxygen probe con nector 1 Conductiv ity probe con 7 External nector Reference connector for pH measure ments Model pHi 570 Shown other models may not have all the connections shown 21 22 Probe Connections BNC Connector for pH The Model pHi 410 450 460 470 510 550 560 570 meter accepts any pH or ORP electrode with a BNC connector Be sure that both the receptacle on the meter and the connector on the probe are clean and dry
3. pressing the a TIME key will toggle be tween ppm and ppt 5 Press ENTER Repeat Step 4 for each calibration point NOTE To view the currently stored calibration values exit SETUP and go to the ISE calibration mode All calibration values in the currently setup method can be viewed using the Aor VW keys 83 COND Setup 1 Conductivity Calibration Alarm The meter allows a Conductivity recalibration alarm to be set from 1 hour to 1999 hours The default condi tion is 00O no recalibration alarm The setup proce dure is the same as setting the pH recalibration alarm To setup the calibration alarm L 84 Press SETUP then press COND to put in the con ductivity setup mode Press the Aor W until the conductivity calibration alarm setup screen is displayed CAL Press ENTER The display will show the calibra tion alarm status in hours OOO indicates the alarm is OFF Use the numeric keypad or the Aor W to set the calibration alarm in hours Press ENTER to save changes and return to SETUP MODE COND Setup 2 Conductivity Temperature Compensation Coefficient Conductivity is automatically temperature compen sated Default is 2 per C The user selectable range is 0 10 Te Press SETUP then press COND to put in the Con ductivity setup mode 2 Press the Aor W until the Conductivity Tempera ture Compensation Factor screen is displayed 3 Press ENTER The display will
4. C or F Default mode is C To select temperature units l Press SETUP to put in the setup mode 2 Press the Aor VW until the Temperature units setup screen is displayed 3 Press ENTER The display will show the current units Pressing the Aor Vv keys will toggle between C and F C SETUP Use the Aor to set the temperature units 5 Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes General Setup 4 Sound ON OFF 1 Press SETUP to put in the setup mode 2 Press the Aor VW until the Sound icon is displayed Snd SETUP 3 Press ENTER The display will show the Sound status ON or OFF Pressing the Aor V keys will toggle ON and OFF 4 Use the Aor WV to toggle ON or OFF 5 Press ENTER to Save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes 65 66 General Setup 5 Meter Automatic Shutoff Time The meter will shutoff automatically to conserve bat tery power after a predetermined period of time Meter will beep intermittently for one minute prior to shut down User must power cycle for the automatic shutoff to take effect Range 1 minute to always on Default 20 min utes 1 Press SETUP to put in the setup mode 2 Press the Aor VW until the Meter Automatic Shutoff Time screen is displayed 3 Press ENTER The display will show the number of
5. Please see the SmartLogger II manual on the included CD for further SmartLogger II operating instructions a Section 9 Bluetooth Wireless Connectivity All handheld models 410 420 430 450 460 470 and select benchtop models 550 560 570 are equipped with Bluetooth wireless connectivity to a PC Stored data can be uploaded to a PC real time meas urement values can be viewed remotely on a PC and many functions of the meter can be remotely controlled from a PC via Bluetooth 1 When the meter is turned on it will be in discovery mode and will seek a Bluetooth connection with a PC 2 Follow your PC s software instruction guide for op eration of your PC s Bluetooth connection software This usually involves going to a setup connect or Bluetooth menu on your PC 3 On your PC select the option to discover or add a Bluetooth device Your PC will then ask if you want to pair with it 4 Accept and then you will be required to ENTER a passkey or PIN The PIN 1234 Your PC will confirm successful pairing and your meter will dis play the Bluetooth icon In case of unsuccessful pairing repeat steps 1 through 4 NOTE Go to www BeckmanCoulter com pH for additional information regarding the Bluetooth capabili ties of your meter Bluetooth icon 25 26 NOTE If you have more than one Beckman Coulter Bluetooth meter within range each meter will identify itsel
6. Stored Instrument Data e Meter Serial Number e Software Version 51 52 Section 21 Recalling Stored Calibrations To recall pH calibration data press RECALL while in the pH COND TDS or DO measurement mode Press the CAL key to indicate you wish to access calibration data The number of the most recently stored calibration data location will be displayed 9 RECALL CAL Uo To view this data press RECALL To select a dif ferent memory location use the numeric keypad to enter the location number then press ENTER The first calibration point for the stored calibra tion will be displayed Use the Aor VW key to scroll to view other stored calibration points RECALL CAL To view the mV value of the stored calibration point press ENTER To view the slope of the Stored calibration press ENTER again Press RE CALL or CANCEL to return to viewing stored pH calibration values To view the time of a stored calibration press the TIME key Press the TIME key again to display the date Press CANCEL or the pH mV ISE TDS SAL DO key to return to the measurement mode 53 54 SECTION 22 Setup Functions General Navigation for setup options 1 Press SETUP to start General Setup Functions or SETUP then pH COND TDS SAL or DO to start Parameter Setup Functions A passcode may be required if the System Administrator has previously set a passcode Press the Aor VW keys to scroll to the setup functi
7. The display will show the calibra tion alarm status in hours OOO indicates the alarm is OFF Use the numeric keypad or the Aor W to set the calibration alarm in hours Press ENTER to save changes and return to SETUP MODE ISE Setup 2 Setting Up for 2 3 4 or 5 Point I SE Calibration To select number of calibration points Default is 2 point 1 Press SETUP then press ISE to put in the pH setup mode 2 Press the Aor VW until the pH calibration point setup screen is displayed 3 Press ENTER The display will show the current number of calibration points Use the Aor VW to set calibration points options are 2 3 4 or 5 points Pressing the Aor V keys will scroll between 2 3 4 or 5 4 Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes 81 82 ISE Setup 3 ISE Calibration Method Setup The meter allows you to define which ISE standards are to be used and in which order To setup the first point of an ISE calibration method 1 Press SETUP then press ISE to put in the ISE setup mode 2 Press the Aor V until the Calibration Method setup screen is displayed 3 Press ENTER The display will show the numeric entry screen 4 Press the a TIME key twice to toggle between ppm or ppt resolution Use the Aor WV keys or the numeric keypad to enter the first ISE calibra tion standard Before entering any digits
8. and wait until wait stops flashing Press ENTER and wait until wait stops flashing If necessary press ISE until icon is displayed When complete the next calibra tion value is shown and CAL 2 is displayed With slope displayed press ISE rinse and place in sample Quick Start Guide Conductivity Calibration Model pHi 430 460 470 530 560 570 COND READY UcD C5 G c ate Connect probe Put probe in Press ON OFF f necessary to meter first calibrat to turn on press COND ing solution meter ont COND icon is seen on the display Press CAL The conductivity Press ENTER When slope is value will flash Press Aor V until display matches calibrating solution displayed press COND rinse probe and place in sample Quick Start Guide Dissolved Oxygen Calibration Model pHi 420 470 520 570 If necessary Connect probe Hold probe Press ON OFF press DO until to meter downward in to turn on DO icon is air meter seen on the The CAL value press ENTER When the A will flash and wait until slope is the wait icon displayed stops flashing Press DO then place in sample TABLE OF CONTENTS Quick Start Guides pH ISE Conductivity and Dissolved Oxygen Calibration Section 1 Safety Precautions and Warranty 0005 SeEClion 2SpeCiiCalONnS ac 2 4 dsn Aspe an se a Sau ey ade Section oo LCD Dis
9. minutes until the meter automatically shuts off 4 Use the numeric keypad or the Aor VW keys to set the shutoff time in minutes Set to 000 for continuously on 5 Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes General Setup 6 Setting the Time L 3 Press SETUP to put in the setup mode Press the Aor V until the Time setup screen is displayed Press ENTER The current time will be displayed in 24 hour format The segment for the first digit will flash Use the number keys to set the time Press EN TER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes 67 68 General Setup 7 Setting the Date Format The meter can be set for mm dd yy format or dd mm yy format 1 Press SETUP to put in the setup mode 2 Press the Aor V until the Date Format screen is displayed 5 Press ENTER Pressing the Aor Y keys toggles month day format 12 31 or day month format 31 12 4 Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes General Setup 8 Setting the Month and Day I 3 4 Press SETUP to put in the setup mode Press the Aor VW until the Date icon is displayed Press ENTER The current date will be displayed The segment for the first digit will flash Use the number keys to
10. Temperature Temp Compensation Coefficient conductivity TDS Temperature Normalization 20 or 25 C TDS Conversion Factor TDS Salinity Adjustment DO Barometric Pressure DO Date Time Meter Serial Number Cell Constant Conductivity Software Version Calibration Date Calibration Time Section 16 Storing Measurements While in the reading mode pH Conductivity TDS Salinity or DO press STORE The meter will display the next available sample number The icon will be flashing To store data in this sample number press STORE again The data will be stored in that memory location and the meter will return to the measure ment mode To store data in a different sample number use the number keys to select a different sample number Press ENTER to store in this memory location The meter will return to the measure ment mode 43 44 NOTE If a sample number already contains data or all memory locations are full the meter will beep twice and prompt to overwrite the data point by displaying DELETE SAMPLE Press ENTER to overwrite the data in that location with the current data or press CANCEL to return to the measurement mode without overwrit ing the data Section 17 Datalogging The Model pHi 400 500 Series meters will datalog up to 999 data points at user selectable intervals from 1 sec ond to 1999 seconds See SETUP functions NOTE If logging titrations or other measurements where the
11. aitala as values when in calibration mode Used to view setup screens Used to set numeric values during setup Turns on the LED backlight for two minutes Starts or stops logging ENTER Used to confirm values to begin calibration Press in measurement mode to HOLD reading HOLD 16 SECTION 5 Instrument Features Sound Sounds will occur as follows e Three short beeps any time measurement stability is reached during calibration mode regardless of the Stabilization Lock setting e One short beep when stabilization is reached when in Stabilization Lock ON mode The READY icon will illuminate NO beep if Stabilization Lock is in the OFF mode e Two long beeps if an error condition occurs An error code will be displayed e Audible click on key tap Automatic Detection of Cell Constant pHi 430 460 470 530 560 570 The meter will automatically detect the cell constant of a conductivity electrode Auto Power Off Will automatically power off 20 minutes default after last key press Auto power off is user selectable from one minute automatic shutoff to always on When datalogging the auto power off is disabled During data transfer by Bluetooth the auto power off is disabled When connected through the USB port the auto power off is disabled LED Display Backlight Display backlight goes on when the light button is pressed The light stays on for 2 minutes after the last keystroke Bluetooth Wir
12. as setting the pH recalibration alarm To setup the calibration alarm 1 Press SETUP then DO to put in the dissolved oxy gen setup mode Press the Aor Wuntil the DO calibration alarm setup screen is displayed Press ENTER The display will show the calibra tion alarm status in hours 000 indicates the alarm is OFF Use the numeric keypad or the Aor W to set the calibration alarm in hours Press ENTER to save changes and return to SETUP MODE DO Setup 2 Dissolved Oxygen Measurement Units Dissolved Oxygen can be displayed in mg L satura tion or ppm 1 Press SETUP then DO to put in the dissolved oxy gen setup mode 2 Press the Aor V until the DO units screen is dis played Pressing the A or Vv keys will toggle between mg L and ppm 4 Usethe Aor YW to set the DO units 5 Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes 95 96 DO Setup 3 Dissolved Oxygen Calibration Units Dissolved Oxygen can be calibrated in metric units of mg L or saturation or ppm 1 Press SETUP then DO to put in the dissolved oxy gen setup mode 2 Press the Aor VW until the DO Calibration units screen is displayed ae Press ENTER The display will show the DO cali bration units Pressing the Aor Y keys will toggle between mg L and ppm 4 Use the Aor VW to set the DO calibration units 5 Press ENT
13. display the slope 11 Press the Bar key to view the barometric pres sure Press SAL to view the measured salinity ad justment if a conductivity salinity probe is at tached If no conductivity probe is attached the manual salinity compensation factor is displayed NOTE On models containing dissolved oxygen conductivity TDS or salinity options models 420 430 460 470 520 530 560 570 you will receive either a beep or a E20 E27 error code if you access any of those functions WITHOUT an attached dissolved oxygen or conductivity probe The meter performs probe self checks prior to entering those measurement modes and because the probe is not connected it is considered an error condition The error codes will not be present when the appropriate Beckman Coulter Dissolved Oxygen or Conductivity Probe is connected 41 42 Section 15 Stored Measurement Data The pHi 400 500 Series meters can store up to 999 measurement readings Data must be stored to recall it for later review downloading or printing The meter will store the following data for each measurement Stored pH and I SE Measurements Sample Number pH or ISE value mV value pH Sample Temperature Temperature Compensation Auto or Manual value Date Time Meter Serial Number Software Version Calibration Date Calibration Time Stored Conductivity TDS Salinity or DO Measure ments Sample Number Conductivity TDS Salinity DO value Sample
14. displayed After pressing ENTER on the last calibration buffer the change will be saved and the meter will return to the SETUP mode Press CANCEL to return to SETUP mode without saving changes pH Setup 5 Manual Temperature Compensation for pH Electrodes pH electrodes are automatically temperature compensated if a temperature probe is detected in the 3 5mm phono jack If no probe is detected the system defaults to manual temperature compensation at 25 0 C Manual temp compensation is always in C No F conversion is necessary 1 Press SETUP then press pH to put in the pH setup mode 2 Press the Aor Y until the Manual Temp screen is displayed 3 Press ENTER The display will show the Manual Temp value Pressing the Aor Y keys will scroll the temperature compensation value up or down 4 Use the Aor V to set the manual temp compensation 5 Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes 79 80 ISE Setup 1 ISE Calibration Alarm To setup the calibration alarm 1 Press SETUP then press ISE to put in the ISE setup mode Press the Aor VWuntil the ISE calibration alarm setup screen is displayed The meter allows an ISE recalibration alarm to be set from 1 hour to 1999 hours The default condition is 000 no recalibration alarm The setup procedure is the same as setting the pH recalibration alarm Press ENTER
15. enter the location number then press ENTER NOTE Unit will indicate no if you attempt to recall from a location in which there is no stored data point If no is displayed use the up or down arrow to scroll to a location with stored data 4 To display the time a stored sample was taken press the TIME key SAMPLE RECALL 5 To display the date a stored sample was taken press the TIME key again To return to the stored measurement value press the TIME key again or press pH ISE COND TDS SAL DO or CANCEL to return to the measurement mode u S c 5 DATE 47 48 Section 19 Deleting Stored or Logged Data l To delete a single sample measurement it must be recalled first Press RECALL while in the measurement mode Press the STORE CAL or LOG key to indicate whether you wish to access stored calibration or logged data The number of the most recently stored data location will be dis played To view this data press RECALL To se lect a different memory location use the numeric keypad to enter the location number then press ENTER See Section Recalling Stored or Logged Data See Section 17 for a full description 258 mC _ SAMPLE RECALL When the desired sample ts displayed press the DELETE key The meter will display DELETE SAMPLE Press ENTER to erase the data If you do not want to delete the data press CANCEL or press pH ISE COND TDS SAL DO to re turn to the measu
16. fastest response time Is required for minute changes in value it is best to turn off stabilization lock READY icon in the SETUP mode 1 While in the reading mode pH Conductivity TDS Salinity or DO press LOG The meter will begin datalogging at the interval determined in the meter setup The LOGGING icon will be flashing and the digits in the lower right will indi cate the number of datapoints collected pH too a a pH l E l Number of o datapoints CIUS Ae logged LOGGING lt 2 To stop logging press the LOG key To resume logging press the LOG key again To prevent the loss of data when the logging memory is full rather than just stop the meter will delete every even numbered data point and will continue log ging NOTE Recalibration alarms are not active during log ging Logging is suspended during calibration and SETUP 45 46 Section 18 Recalling Stored or Logged Data 1 Itis possible to recall stored data or logged data To recall stored or logged data press the RECALL key while in the measurement mode STORE LOGGING RECALL CAL 2 Press the STORE key to recall stored data or press LOG to recall logged data VO ot ro ot 258 C Ac SAMPLE SAMPLE RECALL nng RECALL LILJ 3 The number of the most recently stored data loca tion will be displayed To view this data press RECALL To select a different memory location use the numeric keypad to
17. the parameter requested Turn off the meter Install the correct probe Turn on the meter 107 108 SECTION 24 Troubleshooting Guide INDICATION CAUSE USER ACTION No Display Unstable reading Auto Power Off has cleared display Dirty probe Dirty probe meter connectors Reference junction not flowing Interference from other devices Probe is ina very low ionic strength solution OH or temperature of solution is changing Press on button to turn meter back on No Power Replace AA batteries in meter Follow the probe cleaning procedures described in this manual Clean probe contacts on probe cable connector and on meter with methanol and a cotton swab and let dry completely Reconnect probe to meter Follow warm buffer cleaning procedure Remove other devices from solution Unplug water baths Stirrers etc Stable reading may not be possible Stable reading not possible until pH and temperature is constant INDICATION CAUSE USER ACTION Meter Out of Carefully follow continually calibration calibration displays 2 00 instructions or 19 99 with electrode attached Probe not in Place probe in solution liquid Gently Shake probe to be Sure no air bubbles are trapped on the sensor surface No probe Turn off meter attached Attach pH probe Turn on meter Dirty probe Follow cleaning instructions Damaged Replace probe probe 109 110 I
18. water and place probe in next calibration solution 7 Press ENTER if the correct concentration is dis played or Aor Wto change value NOTE To per form only a one point calibration and not proceed to the second point press ISE or STORE The meter will accept just a one point calibration and display the slope 33 34 8 If ENTER is selected the concentration value will start flashing until calibration is complete 9 If the meter is set for only a two point calibration upon completion of the second point the meter will beep three times and the display will show the Slope and the number of calibration points If the meter is setup for three four or five point calibra tion repeat steps 6 and 7 10 Press ISE or ENTER to begin reading the concen tration value of any solution NOTE The slope is defined as the change in potential when the concentration changes by a factor of 10 e g from 10 ppm to 100 ppm The slope is defined as the ratio of the measured slope and the theoretical Nernst slope of 59 16 mV per decade of change at 25 C for a monovalent ion The slope for a monovlaent ion should be between 90 and 100 The slope for a di valent ion should be between 45 to 50 The meter will display Error E30 if the slopes are not the same sign or not within 25 of each other If the calibration is more than two points the displayed slope is the average Slope for all points SECTION 13 Conductivity and TD
19. wet or dirty connections may cause unstable readings BNC Connector I SE The Model pHi 450 470 550 570 meter accepts any lon Selective Electrode with BNC connector Be sure that both the receptacle on the meter and the connector on the probe are clean and dry wet or dirty connections may cause unstable readings Phono J ack for BNC Probe Temperature Sensors The temperature sensor for BNC probes uses a 3 5 mm phono jack When a temperature sensor is connected the meter will Automatically Temperature Compensate ATC pH or ISE values Conductivity Probe Connector The Model pHi 430 460 470 530 560 570 meter accepts conductivity probes with different cell constants The temperature sensor is built in to the conductivity probe so a separate temperature probe is not required If a conductivity probe is detected conductivity measurement and calibration is enabled The cell constant of the conductivity probe is automatically detected The following cell constants are recommended for the following applications K 0 5 for general purpose K 1 for low conductivity such as drinking water K 10 for salinity or brackish water DO Probe Connector The Model pHi 420 470 520 570 meter accepts the Dissolved Oxygen probe The temperature sensor is built in to the DO probe so a separate temperature probe is not required Reference Probe Connector The Model pHi 510 550 560 570 meter accepts an external reference for pH measurements USB C
20. 0000 NOTE After exiting SETUP the passcode is not en abled for five minutes or until the meter is turned off then turned back on This feature allows the System Administrator while configuring the meter to move quickly between the measurement modes and setup modes without repeated use of the password 61 62 General Setup 2 Setting Stabilization Lock ON or OFF pH conductivity TDS and DO can be displayed with a Stabilization Lock mode When a stable reading is reached the display locks on the value and the meter ignores very slight changes in the measurement The Ready icon turns on when an endpoint is reached and the Stabilization Lock is active The display automatically unlocks after a significant measurement change is detected When performing titrations or attempting to detect very slight changes the Stabilization Lock should be OFF Default is Stabilization Lock ON 1 Press SETUP to put in the setup mode 2 Press theA or VW until the Stabilization Lock screen is displayed 3 Press ENTER The display will show the Stabiliza tion Lock status Pressing the Aor Vv keys will toggle Stabilization lock ON or OFF 4 Use the Aor VW to set the Stabilization Lock mode 5 Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes 63 64 General Setup 3 Setting the Temperature Display to C or F Temperature can be displayed in
21. 70 510 550 560 570 If the meter is in the pH mode and a temperature probe is plugged in to the 3 5mm phono jack the meter defaults to Automatic Temperature Compensation ATC If no temperature probe is detected it defaults to Manual Temperature Compensation at 25 C Manual temperature compensation values are user selectable Conductivity Temperature Compensation pHi 430 460 470 530 560 570 Conductivity is always automatically temperature compensated using the thermistor built into the probe The Conductivity automatic temperature compensation coefficient is user selectable Default is 2 per degree C Dissolved Oxygen Temperature Compensation pHi 420 470 520 570 If the meter is in the Dissolved Oxygen mode a dissolved oxygen probe is attached to the DO connector The meter defaults to Automatic Temperature Compensation ATC All Beckman Coulter DO probes have built in temperature Sensors ISE Temperature Compensation lon Selective Electrodes are not automatically temperature compensated Barometric Pressure Compensation pHi 420 470 520 570 The meter has an on board pressure transducer for automatic barometric pressure compensation of dissolved oxygen In addition the actual barometric pressure can be displayed in inches of mercury millimeters of mercury or hectopascals Table 4 pH Temperature Compensation Tables The table below shows the pH values of buffers at vari ous temperatures
22. ER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes DO Setup 4 1 point or 2 Point Dissolved Oxygen Calibration Default is 1 point at 100 saturation assumes a nominal slope of 47 mV from 0 to 100 at 25 C at 1013 hPa barometric pressure with 0 ppm salinity For highest accuracy always use a two point calibration 1 Press SETUP then DO to put in the dissolved oxy gen setup mode 2 Press the Aor Y until the DO calibration point setup screen is displayed 3 Press ENTER The display will show the current number of Calibration points Pressing the Aor Y keys will toggle between 1 and 2 point 4 Use the Aor W to set calibration points options are 1 or 2 points 5 Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes 97 98 DO Setup 5 DO Calibration Method Setup The meter allows you to define which DO standards are to be used and in which order To setup the first point of an DO calibration method l Press SETUP then DO to put in the DO setup mode 2 Press the Aor VW until the Calibration Method setup screen is displayed DO SETUP CAL i 3 Press ENTER The numeric entry screen will be displayed 4 Usethe Aor V keys or the numeric keypad to en ter the first DO calibration standard 5 Press ENTER If the meter is setup for two DO cali bration points r
23. NDICATION CAUSE USER ACTION Trouble calibrating Display does not stop flashing during calibration Probe sensor Surfaces are dirty or probe needs reconditioning Reference junction not flowing Buffers may be contaminated or expired Interference from other devices in solution such as Improperly grounded stirrers water baths or other devices Battery is low If trouble persists probe may have reached the end of its useful life and may need to be replaced Follow cleaning and reconditioning instructions Follow warm buffer cleaning procedure Recalibrate with fresh buffers Remove any other devices from solution Unplug water baths Stirrers or any other electrical devices near the probe Replace batteries if the battery icon indicates low battery power Replace probe SECTION 25 Device Ratings Power Specifications Supply Voltage Current Power Consumption Batteries Environmental Specifications Temperature ambient Humidity Altitude Installation Categories Pollution Degree Protection Rating Handheld 9vDC 100mA LW Backlight on Bluetooth Active 50mW Backlight off Bluetooth Inactive 4 ANSI 15A or EC LR6 AA Alkaline 5 C to 40 C Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Up to 2000m or Il 1 or 2 All models IP 67 111 112 SECTI
24. ON 26 AC and Battery Power The pHi 400 and 500 Series Meters are powered by either AC or batteries Use only the AC adapter Supplied by Beckman Coulter Before using the adapter check the voltage specified on the label to verify compatibility with the voltage in your area 120VAC or 240 VAC A three wire grounded outlet is recommended Battery Power All meters are battery powered and ship with four 1 5V alkaline batteries AA cells included Fully charged batteries allow for more than 200 hours of continuous operation The system features automatic power shut off which is fully explained in the General Setup 5 Section Meter Automatic Shut Off Low Battery Indicator A low battery icon will appear when approximately 25 hours of battery life remains If the batteries are not replaced after the low battery icon appears the batteries will be eventually be depleted to the level where the instrument cannot reliably be used without the possibility of measurement errors When the batteries are depleted to the point that reliable measurement can no longer be made the large display will read bAt and the instrument will not be useable until the batteries are replaced Battery Replacement CAUTION The following procedure should be performed only by qualified personnel Low battery voltage is indicated by the battery status indicator on the display Replace the batteries whenever low voltage is indicated on the display or if the displa
25. S Calibration The Model pHi 430 460 470 530 560 570 meters measure Conductivity and TDS Total Dissolved Solids Salinity measurements are derived from conductivity Conductivity and TDS Calibration The meter can be calibrated with up to five calibration points If a calibration point is greater than 20 of a previously stored calibration point the previously Stored calibration point will be retained and a new calibration point will be saved If the calibration point is less than 20 of a previously stored calibration point the closest stored calibration point will be replaced If five calibration points are already stored the closest stored calibration point will be replaced with the new calibration data The meter can be calibrated with either Conductivity or TDS standards Stored calibration points can be reviewed in SETUP All calibration data can be cleared using the Clear All Calibration Points function in SETUP NOTE The meter defaults to a temperature compensation coefficient of 2 per C The coefficient is user selectable in SETUP NOTE The meter automatically detects the cell constant Calibration is automatically cleared if the conductivity probe is changed to a probe of a different cell constant 1 Press COND to put in Conductivity mode or press TDS to put in TDS mode 2 Place probe in first standard solution Meter will begin reading the conductivity value 35 NOTE If you wish to clear all previous cal
26. User s Manual M Series 400 and 500 pH and Electrochemistry Meters ot one PEC N paet N TNT TA CE A59443AA May 2008 Beckman Coulter Inc 4300 N Harbor Blvd OULTER Fullerton CA 92835 Connect probe to meter Press CAL Rinse probe in deionized wa ter and place in second cali brating buffer Quick Start Guide pH Calibration and Measurement Model pHi 410 450 460 470 510 550 560 570 Put probe in first calibrat ing buffer If necessary press Aor V key until dis play matches first buffer If necessary press Aor V key until dis play matches second buffer value Press ON OFF to turn on meter Press ENTER and wait until the wait icon stops flashing Press ENTER and wait until the wait icon stops flashing If necessary press pH until pH icon is displayed plete the next calibration buffer is shown and CAL 2 is displayed When slope is displayed press pH rinse probe Place in sample Quick Start Guide ISE Calibration and Measurement Model pHi 450 470 550 570 Connect Put probe in first probe to me Calibrating solu ter tion Press CAL f necessary press Aor V until display matches solution concen tration value Rinse in de If necessary ionized wa press Aor V until ter place in display matches second cal next solution solution value Press ON OFF to turn on meter Press ENTER
27. ange ann woo a PASSCODE SETUP 2 Use the number keys to enter the four digit pass code A unique passcode Is set by the system Ad 24 T J ministrator PASSCODE 3 Press ENTER 58 If the wrong passcode is entered the meter will display Err Press CANCEL to reenter the pass code or press the pH mV ISE TDS COND SAL or DO key to return to the measurement i Ll am 2484 PASSCODE SETUP 59 60 General Setup 1 Setting up Passcode Access Control The meter allows passcode access control for all setup functions If a passcode are used when the SETUP key is pressed the operator is prompted for a passcode in order to access setup functions This allows access to calibration methods and other functions to be con trolled by authorized personnel To setup passcode protection 1 Press SETUP to put in the setup mode 2 Press the Aor VW key until the passcode setup screen Is displayed PASSCODE SETUP 3 Press ENTER The display will ask if you wish to change the passcode Default NO Pressing the Aor Vv keys will toggle passcode access NO or YES ria PASSCODE SETUP 4 Press the Aor W key to set to YES Press EN TER PASSCODE SETUP 5 Use the number keys to enter a passcode When finished press ENTER Press CANCEL to abort without entering a passcode NOTE To allow access to SETUP functions without a requiring passcode set the passcode to
28. barometric pressure units Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes 101 102 DO Setup 7 Salinity Compensation for Dissolved Oxygen Auto or Manual Dissolved Oxygen measurements are sensitive to the salinity of the sample When using the meter with an attached Beckman Coulter conductivity probe in the solution the meter will automatically compensate for salinity If you do not have a conductivity probe at tached to the meter the salinity can be manually com pensated from 0 to 49 9 ppt 1 Press SETUP the DO to put in the dissolved oxy gen setup mode 2 Press the Aor Wuntil the DO Salinity screen is displayed 3 Press ENTER The display will show the manual Salinity compensation value Use the numeric keypad or the Aor VW keys to set the salinity compensation value Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes 103 104 SECTION 23 Error Codes Some error codes such as poor electrode condition can be overridden by the user to allow the instrument to be used until a replacement electrode can be ob tained 1 When an error is displayed first check to deter mine the cause of the error To abort the cali bration or other error causing condition press CANCEL or the pH COND TDS SAL DO key 2 Overriding error codes is not recommended however if y
29. cted If temperature probe is connected replace sensor probe E08 Too long to cali Follow probe cleaning in brate Signal structions Disconnect not stable during stirrers and other AC 105 106 ERROR CAUSE USER ACTIONS CODE Dissolved Oxy gen probe Slope error The slope is less than 60 or more than 140 of nomi nal Dissolved Oxy gen probe off set error The mV reading is more than 10mV from nominal Dissolved Oxy gen probe tem perature error Barometric Pressure sensor error lon Selective Electrode cali bration error Replace DO probe mem brane and fill solution If error persists replace probe Nominal 0 1822 ppm mV Replace DO probe mem brane and fill solution If error persists replace probe Nominal 0 0 mV at 0 saturation and 45mvV at 100 If DO probe is attached when error code is dis played replace probe If no DO probe is not at tached and error code is displayed attach a DO probe Return meter to factory for repair and recalibration Slopes are not the same Sign or slopes are not within 25 of each other Probable user error Recali brate in correct solutions ERROR CAUSE CODE Unrecognized host command No probe in Stalled Invalid input USER ACTIONS Command received from a host PC is unrecognized Use only valid commands The value entered during SETUP is invalid Enter a different value No probe is installed for
30. e times and the display will show the Slope mV offset at pH 7 00 and number of calibra tion points After reviewing the calibration data press pH or STORE to begin reading the pH value of any solution If the meter is setup for three or more calibration points continue with the next step mV offset IN pH 7 00 Number of calibration points completed 10 11 12 LS 14 15 Rinse probe in deionized water and place in next buffer solution If setup for additional calibration points up to five press ENTER Ifthe displayed buffer value is not OK press A or Wto change buffer value or press pH to accept just a 2 point calibration and display pH measurement mode If ENTER was selected in step 11 the buffer value will start flashing until calibration is complete Repeat steps 11 and 12 until the final calibration points are complete When done with the final calibration point calibration is complete The meter will beep three times and display the slope mV offset at pH 7 00 and number of calibration points Press pH or ENTER to begin reading the pH value of any solution NOTE The slope is defined as the change in potential when the pH reading changes by one decade e g from pH 7 00 to pH 8 00 The slope is defined as the ratio of the measured slope and the theoretical Nernst slope of 59 16 mV per decade of pH change at 25 C If the slope on a PH electrode is not between 85 and 102 the
31. eless Communication Bluetooth wireless connectivity allows the viewing of real time data as well as the transfer of stored data to a PC In addition many functions of the instrument can be controlled remotely by a PC via Bluetooth Bluetooth is included in the pHi 410 420 430 450 460 and 470 handheld models and the pHi 550 560 570 benchtop models USB Communication Benchtop meters have USB connectivity capability allowing the viewing of real time data as well as the transfer of stored data to a PC Low Battery I ndicator A low battery icon will appear when approximately 25 hours of battery life remains If the batteries are not replaced after the low battery icon appears the batter ies will be eventually be depleted to the level where the instrument cannot reliably be used without the possibil ity of measurement errors When the batteries are de pleted to the point that reliable measurement can no longer be made the large display will read bAt and the instrument will not be useable until the batteries are replaced pH READY T7007 HEEE P cae ATC The bat tery icon gives about 25 hours of warning Replace batteries soon The bat text Is shown when the batteries are too low to provide reliable measure ment The Instrument IS not use able until batteries are replaced 17 Section 6 Temperature and Barometric Pressure Compensation pH Temperature Compensation pHi 410 450 460 4
32. enter the correct digits and press ENTER when finished 69 General Setup 9 Setting the Year 1 Press SETUP to put in the setup mode 2 Press the Aor VW until the Year icon is displayed 3 Press ENTER The current year will be displayed The segment for the first digit will flash 4 Use the number keys to enter the correct digits and press ENTER when finished 70 General Setup 10 Datalogging I nterval The meter will datalog up to 999 datapoints in intervals raging from 1 second to 1999 seconds The default is 10 seconds 1 Press SETUP to put in the setup mode 2 Press the Aor Y until the Datalogging Interval setup screen is displayed mg III LOGGING SETUP 3 Press ENTER The display will show the datalog ging interval ae g Bau SEL 4 Use the number keys to set the datalogging inter val and press ENTER when finished 71 General Setup 11 Clearing pH Conductivity TDS and DO Calibra tion points It is possible to clear all stored calibration points for all parameters It is important to recalibrate the meter prior to use after clearing all calibration points 1 Press SETUP to put in the setup mode 2 Press the Aor VWuntil the Clear All Calibration Points screen is displayed pH COND SAL TDS DO fbr serue CAL ALL 3 Press ENTER 4 The meter will prompt if you want to clear all calibration points The default is No Pressing the A or Y keys w
33. epeat Step 4 for the second calibra tion point NOTE To view the currently stored DO calibration val ues exit SETUP and go to the DO calibration mode All calibration values in the currently setup method can be viewed using the Aor VW keys NOTE If the DO calibration units are changed in SETUP e g from to ppm the meter will automati cally convert the values entered in this setup procedure to the selected units However the conversions are approximate For maximum accuracy re enter the de sired values in this procedure in the correct calibration units NOTE DO calibrations are not salinity compensated The salinity value from either an attached salinity probe or the salinity value entered in SETUP will be ignored 99 100 DO Setup 6 Barometric Pressure Compensation Units Dissolved Oxygen measurements are sensitive to baro metric pressure The meter has a built in barometer to provide automatic compensation of barometric pres sure The meter can display barometric pressure in inHg inches of mercury mmHg millimeters of mer cury or hPa hectopascals or millibars Default hPa 1 Press SETUP the DO to put in the dissolved oxy gen setup mode 2 Press the Aor VW until the Barometric Pressure Unit setup screen is displayed 3 Press ENTER The display will show the units for barometric pressure Pressing the Aor Vv keys will toggle bewteen hPa mmHg and inHg Use the Aor to set the
34. f with Model Number and Serial Number for exam ple pHi470 SN102755 Blue tooth Data logging to a PC 5 Once a Bluetooth connection is established be tween the PC and meter open the SmartLogger I software contained on the included CD Click on the Find Meter icon to have the software connect to the meter Please see the SmartLogger II man ual on the included CD for further SmartLogger II operating instructions 6 When the meter is communicating with the Smart Logger II software on a PC the arrow icon flashes whenever data is being transferred 7 Full instructions on use of the SmartLogger II Soft ware Is contained in a separate manual located on the CD included with all Beckman Coulter Bluetooth enabled meters Section 10 LIMS Interface A complete list of LIMS interface commands are in cluded on the CD accompanying this product Please work with your technical support group to implement into your specific environment 27 28 Section 11 pH Calibration The meter accepts from one to five calibration points The sequence of buffers and number of calibration points can be defined by the System Administrator e The meter defaults to two point calibration To change the number of calibration points please see page 76 1 Press pH to put in pH mode pH READY IAN TEREI 2500 MC 2 Place probe in first buffer solution NOTE If you wish to clear all previous calibration points s
35. ibration points slopes and offset press CAL then De lete If you wish to update a previous calibra tion with a single point calibration omit this step 3 Press CAL The main display value will begin flashing 4 Press the Aor W key to scroll to the value of the conductivity or TDS standard and press ENTER 5 The main display and the Wait icon will flash At any time during the calibration you may exit the calibration mode by pressing the COND TDS or CANCEL button The meter will abort the current calibration and keep the old calibration data 36 6 Upon completion of the calibration the meter will display the slope as compared to the nominal sen sitivity for a probe of that cell constant and the to tal number of conductivity and TDS calibration points stored in the meter Slope rela Number tive to the of cali nominal bration sensitivity points in the me i ter 7 Press COND or TDS to begin reading the conduc tivity or TDS value of any solution 8 If you wish to have multiple conductivity or TDS calibration points repeat steps 1 5 for up to five calibration points NOTE On models containing dissolved oxygen conductivity TDS or salinity options models 420 430 460 470 520 530 560 570 you will receive either a beep or a E20 E27 error code if you access any of those functions WITHOUT an attached dissolved oxygen or conductivity probe The meter performs probe self checks prio
36. ilay ciate eu ee alee eae eke Re eS Section 4 Keypad Functions 0 000 cee ce eens Section 5 Instrument Features 00 cc eee eee eee eae Section 6 Temperature and Barometric Pressure Compensation Section 7 Probes and Connections 00c cence eens Section 8 USB Connectivity 0 0 0 00 ccc Section 9 Bluetooth Wireless Connectivity Section 10 LIMS Interface 0 0 0 0 0 cece eee eee ees Section Ti pH Calibratori ers seya i et eee een ae tee Section 12 ISE Calibration 0 0 0 00 cece ee Section 13 Conductivity and TDS Calibration Section 14 Dissolved Oxygen Calibration 085 Section 15 Stored Measurement Data 2 0002 Section 16 Storing Measurements 0 0 000 ce eee ee eee Section 17 Datalogging 0 cc eee eee Section 18 Recalling Stored or Logged Data Section 19 Deleting Stored or Logged Data Section 20 Stored Calibration Data 0 ccc eee eee Section 21 Recalling Stored Calibrations 000005 Section 22 Setup Functions 0 0 0c ccc eee eee Entering a Passcode to Access Setup Functions General Setup 1 General Setup 2 General Setup 3 General Setup 4 General Setup 5 General Setup 6 General Setup 7 General Setup 8 Passcode Access Control 25 Stabiliza
37. ill toggle from No to YES SETUP CAL At L 5 Press the Aor V key to toggle to Yes and then press ENTER to clear Press CANCEL to return to Setup mode without saving changes 72 pH Setup 1 pH Calibration Alarm The meter allows a pH recalibration alarm to be set from 1 hour to 1999 hours The default condition is 000 no recalibration alarm To setup the calibration alarm di Press SETUP then press pH to put in the pH setup mode 2 Press the Aor Y until the pH calibration setup screen is displayed Alr SETUP UAL 3 Press ENTER The display will show the calibra tion alarm status in hours OOO indicates the re calibration alarm is OFF 73 74 4 Use the numeric keypad or the Aor W to set the calibration alarm in hours Press ENTER to save changes and return to SETUP MODE pH Setup 2 Setting pH Resolution 0 1 pH or 0 01 pH pH can be displayed in two resolutions 0 1 pH or 0 01 pH Stabilization lock READY icon will occur faster at 0 1 pH resolution To select resolution l Press SETUP then press pH to put in the pH setup mode 2 Press the Aor Y until the pH resolution setup screen Is displayed 3 Press ENTER The display will show the pH reso lution Use the Aor W to set the pH resolution Pressing the Aor V keys will toggle between 0 01 and 0 1 resolution 4 Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP m
38. lopes and offset press CAL then DELETE If you wish to update a previous calibration with a single point calibration omit this step 3 Press CAL The Cal icon will begin flashing pH READY EEEN RERE 258 C ATC CAL 4 If the buffer is correct press ENTER The pH buffer value will flash until calibration is complete If the OH buffer is incorrect press the Aor VW key to se lect another buffer Repeatedly pressing Aor V will display the choices of available buffers 1 68 4 01 6 86 7 00 9 18 10 01 12 45 if on CAL 2 or CAL 3 the meter omits the values already used in previ ous Calibrations NOTE Calibration can be aborted while the pH value is flashing by pressing the pH or CANCEL key to return to the measurement mode 5 When calibration is complete for that buffer meter will beep three times and the display will show a flashing CAL 2 and the next pH buffer value factory default is 4 01 The System Administrator can set the sequence of calibration buffers 29 30 6 9 Rinse the probe in deionized water and place in second buffer solution Press ENTER if OK or Aor VW to change value Press pH to accept a 1 point calibration and return to the pH measurement mode If ENTER is selected the buffer value will start flashing until calibration is complete If the meter is set for only a two point calibration upon completion of the second point the meter will beep thre
39. meter will display Error Code E04 If the calibration is more than two points the displayed slope is the average slope for all points The meter will dis play E03 if the offset from 0 0 mV in pH 7 00 is greater than 30 mV 31 32 Section 12 I SE lon Selective Electrode Calibration The meter accepts up to five ISE calibration standards The values of the concentration standards are first en tered in the SETUP mode Calibration can be aborted at any time by pressing the CANCEL key e The meter defaults to two point calibration To change the number of calibration points please see page 81 1 Press ISE to put in the ISE mode 2 Place probe in first calibration solution NOTE If you wish to clear all previous calibration points slopes and offset press CAL then Delete If you wish to update a previous calibration with a single point calibration omit this step 3 Press CAL The Cal 1 icon will begin flashing NOTE The solution temperature will be displayed if a temperature probe is attached to the 3 5mm phono jack However ISE measurements are not automati cally temperature compensated 4 If necessary press the Aor keys to select the concentration of the ISE standard Press ENTER to begin calibration 5 When calibration of the first point is complete the meter will beep three times and the display will Show a flashing CAL 2 and the next ISE concentra tion value 6 Rinse probe in deionized
40. ne i oa 2 ae elare an ant eames aes 4 SAL Setup 1 Salinity Units naaa aa aaa aaa DO Setup 1 Dissolved Oxygen DO Calibration Alarm DO Setup 2 DO Measurement Units nananana aaa eee ees DO Setup 3 DO Calibration Units 0 00 00 cee DO Setup 4 1 or 2 Point DO Calibration 005 DO Setup 5 DO Calibration Method 0 0 00 eee DO Setup 6 Barometric Pressure Compensation Units DO Setup 7 DO Salinity Compensation Auto or Manual Secon 23 EMO COUGS sy 4 c06 teeta she eee eas eee beens Section 24 Troubleshooting Guide 0 0 00 eee eee Section 25 Device Ratings 0005 cece eee ees Section 26 AC and Battery Power 0 000 cee eee eee Section 27 Technical Support n anaana aaee 70 71 72 73 75 76 77 79 80 81 82 84 85 86 87 89 90 92 93 94 95 96 97 98 100 102 104 108 111 112 114 SECTION 1 Safety Precautions and Warranty IMPORTANT NOTICE SAFETY PRECAUTIONS The International safety symbol displayed above appears on this product and is a reminder to the user that all safety instructions Should be read and understood prior to installation operation maintenance or repairs are attempted on this meter If the meter is used in a manner other than as described the safety and performance of the meter can be impaired No AC adapters other than those specifically included or marketed t
41. o work with this unit have been approved for use MRA DAA AE AM EM ARS TM EMNRE MTHS INES IF THE METER IS USED IN A MANNER OTHER THAN AS DESCRIBED THE SAFETY AND PERFORMANCE OF THE METER CAN BE IMPAIRED WARRANTY Your pHi 400 and 500 series meter is warranted to be free of manufacturing defects for three 3 years from the date of purchase This does not include any defects that are the result of abuse or misuse of the meter Beckman Coulter will at their option repair or replace your meter with a comparable unit This is a limited warranty You may have additional rights under your state laws Batteries are not included in this warranty NOTE This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications IF THE SEAL ON THE BACK OF THE METER IS TAMPERED WITH THE WARRANTY IS IMMEDIATELY VOI DED SECTION 2 Specifications Table 1 Specifications Autorang 5 to 105 C Autoranging ing 0 0 ppm to 199 9 23 to 221 1999 ppt mV to 1999 mV 0 01 pH Autorang ing 0 1 andl c 2 J O y x P
42. ode without saving changes 75 pH Setup 3 Setting Up for 2 3 4 or 5 Point pH Calibration To select number of calibration points Default is 2 point 1 Press SETUP then press pH to put in the pH setup mode 2 Press the Aor VW until the pH calibration point setup screen is displayed 3 Press ENTER The display will show the current number of calibration points Use the Aor VW to set calibration points Pressing the Aor V keys will scroll between 2 3 5 or 5 4 Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes 76 pH Setup 4 pH Calibration Method Setup The meter allows you to define which pH buffers are to be used and in which order The default is a two point calibration pH 7 00 for the first point and pH 4 00 for the second point To setup the first point of a pH calibration method l Press SETUP then press pH to put in the pH setup mode 2 Press the Aor VW until the Calibration Method setup screen is displayed 3 Press ENTER The display will show the pH buffer for the first calibration point CAL 71 78 To change to another buffer value press the Aor Vv to scroll thorough the available buffers Press ENTER to set the first calibration buffer The display will show the pH buffer for the next calibration point Repeat Steps 4 and 5 until all buffers are set Pre viously selected buffers will not be
43. on you want to change Press ENTER to indicate you want to change this setting Press the Aand keys to scroll through the choices available for that setup function Press ENTER to store the new setting in memory Press SETUP to return to the measurement mode NOTE Setup is disabled during calibration TABLE 5 SETUP FUNCTIONS SETUP MODE OPTIONS GENERAL Passcode Required NO or YES If YES then set four digit passcode Default NO pH COND TDS DO ON or OFF Stabilization Lock Default ON 3 Temperature Units Cor F Default C Sound ON or OFF Default ON Automatic Shutoff Time Shutoff time in minutes 1 to always on Enter 000 for continuously on Default 20 min Set Date Format mm dd yy dd mm yy Default mm dd yy Set Month and Day Set to current date Datalogging Interval 1 sec to 1999 sec Default 10 sec Clear all pH conductivity NO or YES TDS and DO calibration Default NO points 55 56 TABLE 5 SETUP FUNCTIONS Continued SETUP MODE OPTIONS pH pH Recalibration Alarm Never to 1999 hours Default Never pH Resolution 0 1 or 0 01 Default 0 01 pH Calibration 2 3 4 or 5 point Default 2 point pH Method Define calibration sequence up five buffers pH Manual Temperature User selectable Compensation Default 25 C ISE Recalibration Alarm Never to 1999 hours Default Never ISE Calibration 2 3 4 or 5 point Default 2 point ISE Method Define calibration sequence up fi
44. onnector The Model pHi 510 520 530 550 560 570 meter accepts a standard USB cable AC Power Connector All models accept an AC adapter Use only Beckman Coulter specified adapters NOTE All of the connectors on the handheld meter including the BNC connector and the 3 5mm phono jack are sealed to protect flooding the interior of the meter in the event of submersion If the meter is Submerged and the rubber plug is not installed on the 3 5mm phono jack the interior of the jack may fill with water although the interior of the meter itself remains sealed If the 3 5mm phono jack is flooded it must be dried immediately Best solution is to invert the meter to allow any water to run out of the connector 23 24 SECTION 8 USB Connectivity All benchtop models are equipped with USB connectivity to a PC Stored data can be uploaded to a PC real time measurement values can be viewed remotely on a PC and many functions of the meter can be remotely controlled from a PC via the USB connection 1 With the meter off connect the USB port on the 3 back of the meter to a USB connection ona computer using the USB 2 0 A B Cable as shown below Follow your PC s software instruction guide for operation of your PC s USB connection software USB drivers are contained on the included CD Turn the meter on and open the SmartLogger II software contained on the included CD Click on the Find Meter icon to connect to the meter
45. ou are sure you want to override press ENTER Then as a second step to over ride press the A arrow to toggle the display to YES This indicates you wish to perform and error override Finally press ENTER Pressing the Aor Vv keys will toggle between NO and YES NOTE It is not possible to override all errors NOTE If the meter is in the Conductivity or DO mode and the meter is turned off and probes removed when turned back on the meter will display an error state ERROR CAUSE USER ACTIONS CODE pH electrode off Follow electrode cleaning set error The instructions If error per mV reading in pH sists replace probe To 7 00 buffer is override the error press greater than the ENTER key then select 0 0 mV 30 mV YES for the temporary use of a damaged electrode pH electrode Follow electrode cleaning Slope error The instructions If error per Slope is less than sists replace the elec 85 or over trode To override the er 102 of 59 16 ror press the ENTER key mV per pH unit then select YES for tempo rary use of a damaged electrode calibration power sources Be sure calibration solution tem perature is constant If error persists replace probe Extremely low Replace battery immedi battery ately Meter accuracy and function may be compro mised Bluetooth Ini Return for service tialization Error Damaged Con Connect conductivity probe ductivity probe if probe is disconne
46. r to entering those measurement modes and because the probe is not connected it is considered an error condition The error codes will not be present when the appropriate Beckman Coulter Dissolved Oxygen or Conductivity Probe is connected 37 38 Section 14 Dissolved Oxygen Calibration The Model pHi 420 470 520 570 can be calibrated for dissolved oxygen using either of two methods the wa ter saturated air or the water sample with known DO concentration method Up to two calibration points can be made at any concentration Ifa one point calibra tion is made the meter will replace the previously stored calibration point that corresponds most closely to the new calibration point e g a new 100 DO single point calibration will replace the previously stored cali bration point that is closest to 100 DO value One or two point calibrations can be made in either method Calibration and measurement units can set to or mg L during SETUP e Water saturated air method The best standard operating procedure is to use a narrow necked bot tle such as a BOD bottle Add a small amount of water 1 cc Close the bottle and shake vigorously for several minutes then insert the probe for cali bration A faster approach though slightly less ac curate is to wet the probe membrane and hold the it in air with the sensor facing down Either ap proach gives 100 saturation The default calibra tion unit is e A water sample with a known di
47. rement mode DELETE SAMPLE E Deleting All Stored or Logged Measurements 1 To delete all sample measurements a sample measurement must be recalled first To recall data press RECALL while in the measurement mode Press the STORE key or the LOG key to indicate whether you wish to access stored or logged data The number of the most recently stored data location will be displayed To view this data press RECALL See page 44 Recalling Stored or Logged Data pH READY TO a OF 258 C are SAMPLE RECALL 2 Whena sample is displayed press the DELETE key The meter will display DELETE SAMPLE pH READY 5 Lt l lhal l P Gye ATC DELETE SAMPLE g 49 50 3 4 Press the O key then ENTER The meter will display an ALL Press ENTER to delete all stored samples or press CANCEL to return to the recall sample screen without deleting Section 20 Stored Calibration Data The meter will store the last 10 calibrations The meter will store the following data for each calibra tion Stored Calibration Data e Calibration Number Cal 1 value Cal 1 mV value Cal 1 Temperature Cal 2 value Cal 2 mV value Cal 2 Temperature Cal 3 value Cal 3 mV value Cal 3 Temperature Cal 4 value Cal 4 mV value Cal 4 Temperature Cal 5 value Cal 5 mV value Cal 5 Temperature Slope Cal 1 Cal 2 Slope Cal 2 Cal 3 Slope Cal 3 Cal 4 Slope Cal 4 Cal 5 Cal Date Cal Time
48. robe dependent Accuracy Up to five points Calibration Conductivity Salinity Dissolved Barometric Oxygen Pressure Autoranging 0 00 to 19 99 uS 20 0 to 199 9 uS 200 to 1999 US 2 00 to 19 99 mS 20 0 to 199 9 mS 1 full scale or 1 digit Up to five points Autoranging 0 00 9 99 ppm 10 0 99 9 ppm 100 999 ppm 1 00 9 99 ppt 10 0 99 9 ppt 100 200 ppt Will also display in mg L 0 00 199 9 mg l 200 1999 mg l 2 00 19 99 g l 20 0 50 g l 1 full 0 1 ppt scale or 1 2 to 35 digit C Up to five None points derived from con ductivity 0 0 to 199 9 saturation 0 to 19 99 ppm or mg l Salinity Correction Automatic Barometric Pressure Compensa tion Auto matic 1 5 full scale Two point user select able to any value 225 900 1mm Hg or 1 hPa 0 01 inHg Factory calibration 11 12 SECTION 3 LCD Display 2 3 1 4 pH COND SAL TDS DO 5 m 26 MOLD READY ea 7 ap a ppt 25 E T mV 2 am aD pH o 10 24 j l mS 11 a s a s a us 12 O 0 a S LAY w ba STOR 14 PASSCODE DATE K2 SGGING m 7 22 SERUP AOS 20 18 16 21 19 17 15 Table 2 LCD Display E The meter is in the pH measurement mode The meter is in the Conductivity meas urement mode The meter is in the Salinity measurement mode The meter is in the TDS measurement mode The meter is in the DO measurement mode
49. s SETUP then press COND to put in the Conductivity setup mode 2 Press the Aor Y until the Conductivity Temperature Normalization screen is displayed 3 Press ENTER The display will show the Conductivity normalization value 250C ATC SETUP 87 88 Use the Aor to toggle 20 0 C and 25 0 C Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes COND Setup 5 Conductivity Probe Cell Constant This screen displays the cell constant of an attached conductivity probe If no conductivity probe is attached the display will read 000 To view the probe constant 1 Press SETUP then press COND to put in the con ductivity setup mode 2 Press the Aor Wuntil the conductivity probe con stant screen is displayed 3 Press ENTER to return to SETUP mode 89 90 TDS Setup 1 Setting the TDS Conversion factor TDS values are related to conductivity The operator can calibrate using a TDS standard or calibrate using conductivity standards then program the meter with a conversion factor The conversion factor range is 40 to 1 00 The default is 50 To set the TDS conversion factor l Press SETUP then press TDS to put in the TDS setup mode 2 Press the Aor VW until the TDS conversion factor screen Is displayed TDS SETUP 3 Press ENTER The display will show the current conversion factor Use the Aor VW to set the conver
50. shing Calibration units can be ppm or mg l as de fined in SETUP 39 40 6 If necessary press the Aor W keys to select the concentration of the DO standard Press ENTER to begin calibration 7 The DO value and the Wait icon will flash until calibration is complete At any time during the calibration you may exit the calibration mode by pressing the CANCEL button The meter will abort the current calibration and keep the old calibration data 8 When calibration of the first point is complete the meter will beep three times If setup for one point calibration the meter will display the slope Press DO or STORE to go to measurement mode 9 Ifthe meter is setup for two point calibration the display will show a flashing CAL 2 and the next DO concentration value defined in SETUP NOTE Slope is the ratio of the actual sensor mV output compared to the nominal sensitivity of 0 0 mV at 0 saturation and 45 0 mV at 100 100 defined as 8 2 mg L saturation at 25 C at 1013 25 hPa barometric pressure at 0 ppm salinity 1mg L 1 ppm The nomi nal slope is 8 2 ppm 45mvV or 0 1822 ppm per mV 10 Press ENTER to begin calibration the DO value and the Wait icon will flash until calibration is complete Upon completion the slope is dis played NOTE To perform only a one point cali bration and not proceed to the second point press DO or STORE The meter will accept just a one point calibration and
51. show the Conduc tivity temperature compensation factor COND Jie LL C ATC 4 Usethe Aor to set the compensation coeffi cient 5 Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes 85 86 COND Setup 3 Conductivity Used Calibration Points The meter stores up to five calibration points Stored conductivity calibration points can easily be viewed and are displayed in order of lowest to highest conduc tivity value 1 Press SETUP then press COND to put in the Con ductivity setup mode 2 Press the Aor Y until the Conductivity Calibration Review screen is displayed CAL 3 Press ENTER The display will show the first calibration point currently being used Press the Aor Y arrow to scroll to view other currently used calibration values NOTE will be displayed if there are no stored conductivity calibration points COND Setup 4 Conductivity Temperature Normalization Conductivity varies greatly with temperature The conductivity Automatic Temperature Compensation ATC adjusts conductivity measurements to factor out the conductivity changes in the reading caused by temperature The readings are referenced to or normalized at a standard temperature The options are 20 C or 25 C The ATC will give the equivalent conductivity or TDS of a solution normalized at 20 C or 25 C Default 25 C 1 Pres
52. sion factor Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes 91 92 TDS Setup 2 Setting TDS Units mg L auto ranging to g L or ppm auto ranging to ppt TDS can be displayed in metric units mg L g L or ppm ppt 1 Press SETUP then press TDS to put in the TDS setup mode 2 Press the Aor Y until the TDS units screen is displayed 3 Press ENTER The display will show the TDS units Pressing the Aor V keys will toggle between mg L and ppm 4 Use the Aor W to set the TDS units 5 Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes SAL Setup 1 Setting Salinity Units ppm ppt or l Press SETUP then SAL to put in the salinity setup mode 2 Press the Aor YV until the Salinity units screen is displayed 3 Press ENTER The display will show the Salinity units Pressing the A or Vv keys will toggle between ppt and Use the Aor V to set the Salinity units 5 Press ENTER to save changes and return to SETUP mode Press CANCEL to return to SETUP mode without saving changes NOTE The ppt will autoscale to ppm as required 93 94 DO Setup 1 Dissolved Oxygen DO Calibration Alarm The meter allows a DO recalibration alarm to be set from 1 hour to 1999 hours The default condition is 000 no recalibration alarm The setup procedure is the same
53. ssolved oxy gen concentration method In this method the concentration must be determined by using a Winkler titration or other technique In either method a near zero dissolved oxygen solution can be obtained by placing the probe into an oxygen scavenging solution such as 20 sodium sulfite for five minutes Dissolved Oxygen is dependent upon barometric pres sure The meter has a built in barometer that will per form automatic compensation for barometric pressure Dissolved Oxygen is dependent upon salinity Salinity compensation is automatic with Model pHi 470 570 if a conductivity salinity probe is attached alternately the salinity compensation factor can be manually en tered in SETUP If using the water saturated air method prepare the first standard by putting 1cc of water in a nar row necked bottle Shake vigorously Blot the probe tip dry then place the probe in the bottle or if using the quick method wet the probe membrane with water and then hold the probe in air with the sensor facing down Press DO to put into Dissolved Oxygen mode The meter will begin reading the DO value Us l EESE 36 gC ATC Place probe in first calibration solution or satu rated air bottle If you wish to clear all previous calibration points slopes and offset press CAL then Delete If you wish to update a previous calibration with a single point calibration omit this step Press CAL The Cal icon will begin fla
54. tion Lock ON or OFF 005 Temperature Display C or F 00 Sound ON OLOF Rrape oe eek hat Rete ses Meter Automatic Shutoff Time WMG fee uhh Gta oe wees ee ae eta Mee ees Dale Format sadean aaa ethan ee A Monhand Day eritre E hese Ae Pa eset ove General SGiup 0 Y Cals tp han eWeek Her koh bia Bene Bee ee ee hak Sa General Setup 10 Datalogging Interval 000 eee General Setup 11 Clearing pH COND TDS amp DO Calibration Points pH Setup 1 pH calibration Alarm 1 2 0 ce ee pH Setup 2 pH ReESOIUNONss seu etc ke eS ee Oh ak heer Seek y Sos pH Setup 3 2 3 4 or5 Point pH Calibration pH Setup 4 pH Calibration Method cc eee eee eee pH Setup 5 Manual Temperature Compensation TOF Glass ElCCiOdeS ions 8 ke ele eee he 8 eres a ue tok es ISE Setup 1 ISE Calibration Alarm 0 0 0 0 cece eee ee ISE Setup 2 2 3 4 or 5 Point ISE Calibration ISE Setup 3 ISE Calibration Method 2 222 00008 COND Setup 1 Conductivity Calibration Alarm COND Setup 2 Conductivity Temperature Compensation Coefficient COND Setup 3 Conductivity Used Calibration Points COND Setup 4 Conductivity Temperature Normalization COND Setup 5 Conductivity Probe Cell Constant TDS Setup 1 TDS Conversion Factor 00000 eee TDS Setup 2 FOS UNIS a wa
55. ve concentrations SETUP MODE OPTIONS COND Conductivity Recalibration Never to 1999 hours Alarm Default Never Conductivity TDS Automatic User selectable Temperature Compensation Default 2 per degree C Coefficient SETUP MODE OPTIONS I SE TABLE 5 SETUP FUNCTIONS Continued 3 Currently Used Conductivity Displays up to five conductivity Calibration Points calibration points Conductivity temperature 20 Cor25C normalization Default 25 C 5 Conductivity Probe Cell Shows cell constant value 000 Constant if no probe SETUP MODE OPTIONS TDS 1 TDS Conversion User selectable 0 4 to 1 0 Factor Default 0 5 2 TDS units mg L or ppm Default mg L SETUP MODE OPTIONS SAL 1 SAL units ppt or Default ppt SETUP MODE OPTIONS DO 1 DO Recalibration Alarm Never to 1999 hours Default Never 2 DO Measurement Units mg l or ppm Default 3 DO Calibration Units mg l or ppm Default DO Calibration 1 or 2 point Default 1 point 5 DO Method Define calibration sequence of one or two concentrations DO Barometric Pressure inHg mmHg hPa Units Default hPa attached or manually set to user defined value 0 to 49 9 ppt DO Salinity Compensation Auto if conductivity probe 57 ENTERING A PASSCODE TO ACCESS SETUP FUNCTIONS IF SETUP FUNCTIONS ARE PASSCODE PROTECTED 1 If you press the SETUP key and are prompted for a passcode the meter setup functions have been protected and require a passcode to view edit or ch
56. y is blank when the meter is turned on To replace the batteries 1 Turn the meter off and disconnect all electrodes 2 Turn the meter upside down 3 Use a Phillips screwdriver to unscrew the screws on battery cover Carefully remove the battery cover Remove the old batteries Check the and markings in the battery compartment and on the new batteries Insert the new batteries as indicated NOTE Be careful not to touch the contacts on the battery or the battery terminals in the meter 7 Replace the cover and secure by tightening the screw AC Power Alternatively the meters can be powered by a 9 Volts 100mA AC adapter 113 114 SECTION 27 Technical Support If you experience problems with your Beckman Coulter meter that these procedures cannot resolved call your local Beckman Coulter dealer In the U S please call the toll free Product Support Line at 800 742 2345 115 NSAI I S EN ISO 9001 Revision 1 10 gt BECKMAN COULTER SIMPLIFY Beckman Coulter Inc 4300 N Harbor Boulevard Box 3100 Fullerton California 92835
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