HT200H User manual Vr1.0
Contents
1. From this point the unit will enter the main menu Run Time Menu 3 8 Switching off Before switching off the instrument verify that e The unit is in the main menu Run Time Menu e The instrument is on hold status STAND BY If the instrument is sampling interrupt it by pressing STOP then EXIT If you are in a menu different from Run Time Menu you have to go back to it before proceeding to switch off the instrument In an emergency you can switch off the instrument from any position main menu secondary menu HTA SMI Oct 2002 23 HT200H User Manual ciel START UP 4 0 Introduction To configure the instrument for use with different vials syringes and analysers it is necessary to use the SET UP Mode Warning The Set Up must be done carefully by trained qualified personnel Incorrect setup may damage the syringe or even the instrument If you don t input values in each field of the Set Up menu the light will remain on even after you exit the Set Up menu 4 1 Set Up Mode To access to the Set Up menu it is necessary to be in the initial screen This can be accessed by pressing exit from any screen in the run menu The setup mode is laid out as in the following flow diagram HTA SMI Oct 2002 24 HT200H User Manual PL ND TU TR Ovn Teh e lt gt lt gt lt gt lt gt lt gt EXIT HT200H Series SET Manual Operation Software Rel 1 00 Syringe size Sample Size lq Sample Depth2. HT200H User Manual IN SYNCHRO INJ MODE 7 2 5 The Injection synchronisation describes the timing of signal that the unit sends to the analyser so that the retention times can accurately be measured The following choices are available Type of Timing of output to synchronisation analyser At the beginning of the Normal syringe plunger movement In the moment it touches A start the injector At the end of the plunger The unit requires a ready signal from the GC to start the injection this can be triggered manually by grounding pin 6 on the GC connector 7 2 6 IN MODE The injection mode is fixed to normal for this injector some GC units are equipped to inject into 2 different injection ports HTA SMI Oct 2002 47 HT200H User Manual 7 3 Single injections Select Single Injection from the run time menu by using the down and right direction keys see page 45 for the flow diagram of the RunTime Menu The screen should now show Method Number XX Sample Number YY Use the keys to select the method number for the injection that you wish to use and the sample position that needs to be injected and press START If a single injection is not required press EXIT to return to the main Run Time menu The following screen will then appear Sng Met Smp Status Sng indicates that a single injection is running in automatic mode this shows the step of the sequence Met is the method number SMP
3. HT200H User Manual nater INSTRUMENT DESCRIPTION 2 1 Parts Definition Retro A A 1 2 4 A A Fronte 3 Table 1 D Standard Version with 40 vial tray TRAY vial plate 40 positions TOWER Contains the syringe CONTROL PANEL INJ ECTI ON PORT LOCATION OF GC OVEN SHAKER U1 BRWNe gt Fixing holes to secure the sampler to the GC Bracket HTA SMI Oct 2002 5 HT200H User Manual 2 1 1 KEY BOARD The control panel of the unit is laid out as follows 0 Table 2 KEY BOARD Display 20 characters 2 lines Keys 1 JaA WN NO SET enters Set Up mode this can only be done from the initial boot up screen MET Enter Method mode SEQ Enter Sequence mode STOP Stops the unit when running START Starts a run once a single injection or Automatic run has been selected EXIT Exits current screen also used like a back key ENTER Confirmation of command O directional key directional key 10 directional key 11 directional key Led A ST BY status indicator B RUN status indicator C A INJ status indicator D B INJ status indicator HTA SMI Oct 2002 6 HT200H User Manual 2 1 2 CONNECTIONS The connection panel is on the rear of the instrument Table 3 CONNECTION PANEL 1 Power setting 115 230 Vac 2 1 0 Power switch Connectors 3 Power cable socket 4 RS232C serial port 5 GC Analyser connection GC e
4. 4 Insert the fuse holder displaying the correct voltage at the top 5 Close the compartment by pushing gently HTA SMI Oct 2002 18 HT200H User Manual 3 3 Autosampler Assembly Ensure that the unit is not plugged into the power Unpack the instrument carefully Remove the belt from around the tower that is used to protect the instrument during transportation e Check from the product specifications if the instrument has the tray on the left or on the right hand side e Remove the two screws used to fix the plate on the base See 2 1 4 e Fix the tray support using these two screws a Location of the tray support e Connect the cable from the tray support to the socket underneath the base e Put the instrument upright on a suitable flat bench HTA SMI Oct 2002 19 HT200H User Manual 3 4 Installing the unit onto the analyser Warning Follow these instructions with the instrument switched off Please follow these instructions in order 1 Check the sampler is configured correctly with the tray on the right hand side or on the left hand side 2 Fix the bracket onto the analyser For the correct installation see the instructions with the bracket each analyser has a specific bracket and installation instructions 3 Place the sampler onto the bracket ensuring that the four holes identified with A in table 1A correspond with the bracket holes 4 Secure the auto sampler to the bracket using
5. OVN is the oven The and 9 keys will then move the parts in the following way lift 0 or lower Q the syringe piston lift 0 or lower 6 the syringe holder rotates tower to the right 9 or left 0 rotates the tray to the right 9 or left 0 Open 0 or close 6 the oven The symbol under the word TCH indicates the injection sensor status to warn the operator that the needle guide has touched a surface The needle guide is touching a surface The needle guide is not touching a surface HTA SMI Oct 2002 26 HT200H User Manual 4 3 Actual Parameters In this mode it is possible to view the set up parameters as they are currently installed The following parameters can be viewed Syringe size Sample Size Sample Depth Injection Speed Injection depth These values can not be set from this screen they can only be viewed For the injection speed the following table can be used to convert the set value to a speed in mm sec Corresponding Speed Corresponding Speed oO ___2mm s mms O1 mm s 6 128 mm s 2 _ 8mms 7 256mms 3 16 mm s 8 512 mm s 432 ms HTA SMI Oct 2002 27 HT200H User Manual 4 4 Injector Align Attention It is necessary to have the syringe installed following all the relative procedures before proceeding The display shows Needle in SPEED Injector Align Needle in SPEED allows descent speed of the needle
6. Dwl btw Inj is the dwell time between the consecutive injections This allows the equilibrium between the vapour and liquid in the vial to stabilise and also can prevent cavitation if large volumes are withdrawn from the vial I NJ ECT spd mim FLUSH Time 6 2 13 The injection speed is the speed at which the sample is injected into the GC This can have value between 0 1 100ml min From 0 10ml the speed can be set in units of 0 1ml min From 10 100ml min the speed is settable in units of 1ml min The flush time is the length of time the syringe is flushed after the injection The gas line at the rear of the unit has to be connected in order for this to work HTA SMI Oct 2002 43 HT200H User Manual Pre Inj Dwl sec Post Inj Dwl sec 6 2 14 This screen is used to set the Pre and Post injection dwell times The preinjection dwell time is the time when the needle is in the injector before the plunger is lowered The Post Injection Dwell time is the time the needle is in the injector after the plunger is lowered This allows time for less volatile components to evaporate from the syringe Each value can be set for a time between 0 99 seconds For headspace gas analysis these values can normally be set to 0 Press ENTER to Save Copy Method X as Y 6 2 15 This screen asks for confirmation that any changes to a method be saved into memory Pressing ENTER whilst in this screen will save the current method being edited X into position Y
7. Lower syringe holder NDL about 5cm down from its highest position Loosen the safety block allowing it to slide easily 10 Remove the piston block by carefully pulling with 2 fingers 11 Remove the syringe block using the Allen key supplied 12 Raise the syringe holder NDL back to its highest possible position 13 Ensure the appropriate syringe holder is used for the syringe WNE NE There are two different syringe holders suitable for various syringes Before mounting the syringe check that the appropriate syringe holder is installed If the syringe holder is wrong it will either be impossible to insert the syringe or will not allow the syringe to sit safely inside it 14 Insert the needle into the intermediate needle guide and then into the needle guide insert the syringe body into the holder lastly position the plunger into its lodging 15 Fix the syringe block Shut carefully and tighten the two screws 16 Lock the safety block This operation ensures that the needle point remains perfectly aligned with the needle guide and that the needle doesn t protrude out of needle guide This will ensure the safety of the needle 17 Raise the plunger PLG to insert the piston block Insert the piston block taking care to orientate it the right way The correct face should point down 18 Check that the syringe needle is correctly aligned as per the following illustration HTA SMI Oct 2002 31 HT200H User
8. Manual L 7 K NO J Push to return to the menu then select Touch amp Plunger Zero Push to start the procedure 19 Close the Syringe Lodging lowering the lid and locking with the safety screw using the Allen key supplied cfr tables 7A and 7B 20 At the end of the procedure exit from Set Up Mode by pressing the SET key HTA SMI Oct 2002 32 HT200H User Manual 4 6 2 REMOVAL AND REPLACEMENT Removal 1 2 3 4 Ensure that unit is in standby mode Position the tower in central position this is so that it is over the oven Open the Syringe Lodging remove the protective screw and raise the shutter Enter Set Up Mode see chapter 4 select by pressing on the Vial Syringe Setup line 5 Using and enter Syringe Installation Mode 6 Lower the syringe plunger PLG to its lowest point 7 8 9 1 Lower the syringe lodging NDL about 2 cm Remove the piston block using two fingers Remove the syringe block using the supplied Allen key 0 Extract the syringe carefully Replacing the syringe Replace the syringe according to the syringe set up above Ensure that the correct volume is entered and stored in the memory of the sampler HTA SMI Oct 2002 33 HT200H User Manual ua pick SEQUENCE MODE 5 1 Method uences If the sampler is required to proc
9. Met NXT Fill speed too high Met Stp NXT Typical causes of these errors include The sample volume is larger than the syringe Filling volume is larger than the syringe Filling speed higher than available HTA SMI Oct 2002 55
10. Select NXT and press ENTER The display will now show Sequence Step 2 3 Change the method number to 6 then press the down arrow 4 Set the first sample to 3 2 and the last Sample to 4 2 then press the down key 5 Select SEQ to return to the runtime menu 6 From the runtime menu press the down key until the Automatic Injection screen is reached Select this method by pressing the right arrow 10 At the prompt select sequence step 1 as the first sequence step and step 2 as the last sequence step 11 Press START The unit will now run all 15 samples in order HTA SMI Oct 2002 37 HT200H User Manual poets METHOD MODE 6 1 Programming a method The method mode can be accessed from the Runtime menu by pressing the MET key on the keypad The method mode allows the definition of a particular preparation and injection for a sample The flow diagram of the method mode is shown on the following page By using the directional keys the following parameters can be set 6 2 1 Method Number The unit can store up to 10 individual methods in memory The method number can be set from 0 9 6 2 2 Method Type There are two method types Constant C or Progressive P In the constant mode the vials are kept in the incubator for a constant period of time and this is the same for all the samples In the progressive mode the length of time the samples are incubated is increased For example if the Progressive mode is selected and
11. as the manual control screen of the unit Use the control panel to move the tower plunger needle into the correct place and then insert the new needle according to the installation instructions on the following page Touch amp Plunger Zero After installing a new needle or changing the vial dimensions it is necessary to zero the touch point of the plunger The unit will do this automatically once the function has been selected Ensure there is a vial in position 0 1 of the tray as the unit will touch this position If the vial size has been changed the oven will open and a message will appear to either Install Spacers if changing from 20ml to 10ml or Remove Spacers changing from 10ml to 20ml Follow the onscreen instructions and then press start HTA SMI Oct 2002 30 HT200H User Manual 4 6 Syringe I nstallation In order to avoid damaging the syringe or the sampler please follow the instructions carefully and in order 4 6 1 INSTALLATION Check that unit is in standby status Ensure the tower is in the central position Open the Syringe Compartment removing the safety screw with the Allen key supplied with the instrument and raising the protective lid Enter the SET UP Mode refer to 4 1 using select Vial Syringe Setup Ensure the Syringe Size is set to the correct value Using and enter the syringe installation mode Lower the plunger PLG into to the lowest position possible
12. in memory Note The unit will automatically overwrite any previous method without asking for confirmation Once the method has been stored in memory press MET to return to the runtime menu To exit from the Method without saving the modifications press the EXIT key twice then press MET This works from any screen in the Method menu HTA SMI Oct 2002 44 HT200H User Manual CHARS RUN TIME MENU 7 1 The run time mode is the main mode from which the injections can be made After the unit has been powered up and the test sequences completed the unit will automatically enter the Run Time Mode A flow diagram of this mode is displayed on the following page with each parameter being accessible using the direction keys in the normal way VIAL SIZE 7 2 1 This displays the vial size that the unit has been configured for either 10ml or 20ml If a different vial size is required to the one shown the size must be changed in the SETUP mode TURRET ON 7 2 2 This shows the current position of the Turret tower It can be moved using the left right keys to one of three positions to allow easy access to samples or to allow maintenance of the GC Tray over the vial tray Oven This is also Known as the central position Injector This can be used to check the alignment of the injector port ST BY SYR ST BY OVEN 7 2 3 This is screen allows the standby temperature of the syringe and oven to be set The unit will heat the r
13. is the Sample that is being analysed and Status shows the current status of the unit This may be one of the following The unit is heating the oven syringe up to the temperature required by the method This can be monitored by pressing the right arrow key when the unit is in this mode The display will then show Syringe C Target Temp Actual Temp Oven C Target Temp Actual Temp LOAD The unit is transporting the sample from the tray to the oven UNLOAD The unit is transporting the sample from the oven to the tray The sample is being conditioned according to the instructions in COND gt the method The remaining conditioning time can be seen by pressing the right arrow FILL The syringe is being filled with sample INJ ECT The unit is in the injection process WAI TGC The unit is waiting for the ready signal from the GC ABORT The unit is aborting the run after user intervention Once an injection run has started there should be no need for user intervention unless an error occurs see the error list at the back of this manual To break an injection sequence mid run press the STOP key The unit will then require confirmation press EXIT to confirm or START to continue HTA SMI Oct 2002 48 HT200H User Manual On confirmation of the abort the unit will stop the sample preparation and return the vial from the oven to the rack 7 4 Automatic Run In order to perform an automatic run a sequence must
14. made by the plunger prior to taking the sample In this way it is possible to remove air bubbles from liquid samples and also to ensure that the syringe has been completely filled with sample rather than air The number of strokes can be set to between 0 15 The volume of the stroke is defined in the previous screen FILL volume see above The equilibrium delay is the time the plunger waits at the top of its stroke This allows viscose samples to rise through the needle The equilibrium delay will apply to the pullup strokes as well as to the sample withdrawal The delay can be between 0 60secs For normal headspace analysis this value is set to 0 HTA SMI Oct 2002 42 HT200H User Manual Sample Volume Sample speed 6 2 11 The sample volume is the volume of sample that is withdrawn from the vial and injected into the GC The maximum sample volume will vary according to the size of the syringe The sample speed is the speed the plunger moves in the syringe This can be set from 0 100ml min For speeds less than 10ml min the value can be set in units of 0 1ml min after 10ml it can be set in units of 1ml Sample Repeat Dwil btw Inj Min 6 2 12 The sample repeat function allows the unit to make multiple injections into the GC The sample repeat value can be set from 1 single injection to 15 When an injection is repeated the number of Pullup strokes and the syringe preFILL will be the same as for the initial injection The
15. the progressive time is set to 10minutes the second sample will be incubated for 10minutes longer than the first The third sample will be incubated for 10 minutes longer than the second and 20 minutes longer than the first In constant mode all the vials will be incubated for the same time To set the increase in time scroll down the menu until the Progressive parameter appears it is between Incubation Time amp Shaker parameters Note This screen does not appear if the constant mode is selected HTA SMI Oct 2002 38 HT200H User Manual 6 2 3 Ana Time Hour Ana Time Min This screen prompts the user to enter the length of time for the GC analysis in hours and minutes This value is used to calculate the optimum timing for preparing the samples in the oven before injection HTA SMI Oct 2002 39 HT200H User Manual Method type is Contsant C where the incubation Method Number 0 9 time is idenictcal for each vial or progressive P Method Type CorP where the incubation time increases for each vial Ana Time Hour 0 23 Set the GC analysis time Ana Time min 0 59 of the sample Prep Time hour fixed Prep Time Min Displays the calculated preparation time of the sample This cannot be changed OVEN CONDITIONING Set the oven temperature Temperature C 40 150 t Incubation Time Hour 0 23 Set the incubation time Min 0 59 fi Set the time for the shaker to be on off The Shaker
16. with the instrument switched on can damage the autosampler HTA SMI Oct 2002 13 HT200H User Manual 2 3 Typographic Notes For simplification each screen will be shown as follows line 1 id line 2 fl screen The symbols indicate the presence of a field that can have different values These can be modified when necessary The symbols DC _ indicate the direction keys 8 9 10 11 that are located in the front of the instrument see table 2 The symbols set Mer SEQ SToP START ExIT and indicate the keys that are located in the front of the instrument 2 4 Command Keys Description 2 4 1 DI RECTI ONAL KEYS line 1 line 2 1 screen The instrument display has two lines The active line will have a blinking cursor To move from one line to another use the 6 keys These also move from one screen to another The 2 keys modify values indicated on a specific line and also move to secondary menus The directional keys 00 C are not active in all screens the icon in the right hand of the screen indicates which keys are active HTA SMI Oct 2002 14 HT200H User Manual 2 4 2 STOP START EXIT AND ENTER KEYS The key is important for moving inside menus In order to move back from a screen to a previous one and also to exit from a secondary menu to the main menu it is necessary to use EXIT STOP jis used to stop the instrument when it is moving This k
17. HT200H User Manual HT200H USER MANUAL Version 1 0 HTA SMI Oct 2002 1 HT200H User Manual Chapter 1 Chapter 2 2 1 NNNN fii eee mK WN 2 1 6 Contents Technical Specifications Instrument Description Part definition Keyboard Oven Instrument Base Vial Rack Syringe Location 2 2 Moving Parts 2 3 Typographical Notes 2 4 Command Keys 2 4 1 2 4 2 2 4 3 2 4 4 Chapter 3 3 1 WwW WWW WW CON O UBRWN Chapter 4 4 1 4 2 4 3 4 4 4 5 4 6 Directional Keys Function keys Mode keys Status Indicators Installation Installation Input Voltage Autosampler Assembly Installation on to the analyser Cable connections Vial tray installation Start up Switching off Start Up Set up mode Manual Operation Actual Parameters Injector Align Vial Syringe Set up Syringe Installation 4 6 1 Installation 4 6 2 Removal Chapter 5 Sequence Mode 5 1 5 2 Method Sequences Making a sequence 5 2 1 Sequence Step 5 2 2 Method Number 5 2 3 First Sample 5 2 4 Last Sample 5 2 5 Sequence step Examples of sequences HTA SMI Oct 2002 HT200H User Manual 7 3 7 4 Chapter 6 Method Mode 6 1 6 2 1 6 2 2 6 2 3 6 2 4 6 2 5 6 2 6 6 2 7 6 2 8 6 2 9 Programming a method Method Number Method Type Analysis Ana Time Prep Time Oven Conditioning Incubation Time Shaker ON Syringe Conditioning Syringe Prefill 6 2 10 Pullup Strokes 6 2 11 Sample Volume 6 2 12 Sam
18. LATION 3 1 Installation Please follow these instructions carefully 1 Check that voltage value is correct see paragraph 3 2 Check and modify voltage 2 Assemble the autosampler see paragraph 3 3 Autosampler Assembly 3 Install bracket and autosampler on to the analyser see paragraph 3 4 Installation on analyser 4 Connect cables see paragraph 3 5 Cable Connection 5 Install the sample tray following points 1 2 3 amp 4 shown on paragraph 3 6 Tray Installation 6 Switch on the instrument see paragraph 3 7 Start Up 7 Put at least one vial on the plate in position 1 8 Input correct parameters for Vial Size and Vial Depth in the menu Vial Syringe Set up see paragraph 4 5 9 Install syringe see paragraph 4 6 1 Before installation 10 Align the injector see par 4 4 HTA SMI Oct 2002 17 HT200H User Manual 3 2 Check and Modify Voltage Check that HT200H is not connected to the main power The input Voltage of the unit is displayed above the power socket at the rear of the unit and is normally set to 230V To modify voltage proceed as follows Table 8A Table 8 B Open the fuse compartment with a screwdriver as shown in table 8A Extract the fuse holder as shown in table 8B Substitute the two fuses with the ones suited for your power supply as indicated in the table below 115v 0 8A T 230v 0 5A T Table 1 W Ne
19. ON shaker will cycle on and off if the incubation Shaker OFF time is longer than the total ON OFF time To leave the shaker OFF set the ON time to 0 SYRINGE CONDITIONING Set the syringe temperature should normally Temperature C 40 170 be above the oven temperature T This parameter indicated whether the syringe SYR preFILL Yes No should push air into the vial before taking the FILL Volume mL sample YES The fill volume is how much air should be injected and is also used in the Pullup strokes Pullup Strokes 0 15 Equilibrium delay sec 0 60 The number of pull up strokes can be increased to prevent bubbles in the syringe The equilibrium delay time is the amount of time the needle sits in the vial before with drawing the sample It will do this for each pullup stroke Sample Vol mL Sample Speed mLm variable The sample Volume is the volume injected into the detector The sample speed is the speed of the plunger during the injection Sample repeat Dwl btw Inj Min 1 15 99 Sample repeat is the number of consecutive injections of one sample into the GC The Dwell time allows the sample to equilibrate between injections 0 INJECT spd mLm FLUSH Time min Set the injection speed and the post injection flush time unit requires a gas connection Pre Inj Dwl sec Post Inj Dwl sec 0 99 0 99 Set the dwell tim
20. T button here returns the unit to the main runtime menu 7 6 INTERRUPTION PROCEDURE The automatic injection sequence can be interrupted at any time by pressing either the STOP or START keys Pressing STOP will stop the sampler immediately and produce the message CONTINUE AUTORUN START EXIT ABORT AUTORUN ENTER OVEN EXHAUST Pressing EXIT will abort the run and the sampler will unload the vials from the oven back to the vial tray Pressing START will resume the injection sequence Pressing START during an injection will give the following choice EXIT CONTINUE START INJECT VIAL STOP UNLOAD VIAL In this way an injection can be made instantly or an incorrect sample can be removed from the oven 7 7 MONITORING THE INJECTION SEQUENCE It is possible to access information on the state of the unit during an injection sequence by pressing the gt key When in WAIT mode this will show the residual time left for the incubation of the sample pressing the lt returns to the previous screen HTA SMI Oct 2002 51 HT200H User Manual Whilst in WAIT mode pressing the gt will show the remaining analysis time of the sample currently in the analyser In HEAT mode the gt key will show the set and current temperatures of the needle and oven respectively HTA SMI Oct 2002 52 HT200H User Manual Chapter TROUBLE SHOOTING In case of unexplained errors please contact your local dealer In dl
21. These parameters EXIT Injection Speed are view only not Injection Depth settable Actual Parameters Injector Align Needle in SPEED Injector Align Injector Alignment Turret lt lt Needle Move the injector head over the top of the injection port until it touches and the display reads Injector Alignment TURRET STO NEEDLE Press ENTER to store the position in memory Injector Alignment TURRET STO DEPTH Vial Size t Vial Syringe setup Set the height of the needle to Vial Depth t the correct depth then press ENTER to store the position Syringe Size Syringe install PL ND TU TR Own Tch A gt gt lt gt lt gt lt gt Touch Zero Plunger Flow Diagram of the SETUP mode HTA SMI Oct 2002 25 HT200H User Manual 4 2 Manual Operations The Manual Operation is used to move manually the syringe syringe piston tower and the vial tray This function is used when e You want to install substitute or remove the syringe e The sampler makes a movement error in one of its functions This will cause an error message to appear on the screen followed by the control screen below PLG NDL TUR TRY OVN TCH 1 1 1 Using the and keys it is possible to select one of the five PLG NDL TUR TRY and OVN Where PLG is the syringe piston NDL is the syringe holder TUR is the tower TRY is the vial tray
22. be programmed into the unit see chapter 5 The automatic Run is selected by pressing the gt Key on the Automatic run line of the Run Time menu 7 4 1 SET UP The first screen that appears is First Step Last Step This asks for the steps in the sequence that you want to run The unit will only run consecutive steps and will only allow the selection of steps that are in the sequence For instance if there are 10 steps in the sequence the first step can have a value of 1 10 The last step must have a value that is at least equal to the first step and be 10 or less Pressing again gives the following screen End seg Output PowerOn Restart 7 4 2 End Sequence output The End sequence output will send a signal to the GC at the end of the complete sequence This signal is sent through the Auxiliary contact The signal will be sent if YES is selected 7 4 3 PowerON restart The powerON restart function allows the unit to continue the sequence run from where it left off in case of the main power supply failing If this function is set to YES then in the event of a power failure the unit will continue where it left on when the power is restored If it is on NO then the unit will not perform any more steps when the power is restored and will move the samples from the oven to the vial tray as if the unit was rebooted HTA SMI Oct 2002 49 HT200H User Manual 7 5 Running the I njection To start the sequenc
23. cases the serial number of the unit will be required Before contacting your dealer please ensure that All cables are connected and haven t worked loose The instructions in the manual have been followed correctly The needle has not been bent and is aligned properly The sensor is working correctly In most cases if a vial falls during transport the problem is due to an incorrectly crimped cap To reduce this problem the crimpers should be regularly checked to ensure that the cap can not turn once it is crimped and that the under edge of the cap is flush with the vial lip Also check that the tower is not hindered by any obstacles and that the grabbers on the end of the arm are clean Error Messages WARNING Touch Sensor does not work properly Cause The injection sensor is not working correctly This could be due to mechanical problems Possible solution Press EXIT The machine will revert to the initial position Press EXIT again so that the initial boot up screen appears then press the SET key and select manual operation From here it is possible to move the instrument manually to try and find the blockage HTA SMI Oct 2002 53 HT200H User Manual Cause Solution Cause Solution Cause Solution Cause TRAY 40 Removed Waiting Tray The Vial tray has been knocked off center or is damaged or missing Ensure that the tray is properly secured Press EXIT to get the instrument to go t
24. e for the pre and post injections when the needle is in the injector Less volatile substances may need longer dwell times for Headspace this can normally be set to 0 Press ENTER to Save Pressing Enter saves the method to memory Copy Method X as X The default memory position will be that set in the top screen Method number However if you wish to save this method in a different HTA SMI Oct 2002 place you can do so using Copy method HT200H User Manual Prep Time Hour Prep Time Min 6 2 4 This screen displays the calculated time taken to inject a sample It is the combination of the movement of the syringe and plunger with their associated dwell times It does not include the incubation time of the sample When this screen is accessed it will appear only for a few seconds before changing to the next parameter Oven conditioning Temperature C 6 2 5 This is the first screen of the oven conditioning parameters The temperature of the oven can be set between 40 150 C When the injection run is started the oven will heat to this temperature from its stand by temperature Therefore if high temperatures are to be used then it is recommended to have a high standby temperature to save time Incubation Time Hour Min 6 2 6 The incubation time is the time the sample spends in the oven before it is analysed This time can be set from 0 23hrs and 0 59mins Shaker ON Shaker OFF 6 2 7 This allows the shaker to be sw
25. e of injection press the START key from either the above two screens The unit will then begin the sample preparation and will run through all the samples without any further user requirement The unit will display the following Stp Met Smp Status Stp is the current step of the sequence Met is the current method being used for the sample SMP is the sample Status is the current status of the sampler which can be The unit is heating the oven syringe up to the temperature required by the method This can be monitored by pressing the right arrow key when the unit is in this mode The display will then show Syringe C Target Temp Actual Temp Oven C Target Temp Actual Temp LOAD The unit is transporting the sample from the tray to the oven UNLOAD The unit is transporting the sample from the oven to the tray The sample is being conditioned according to the instructions in COND gt the method The remaining conditioning time can be seen by pressing the right arrow FILL The syringe is being filled with sample INJ ECT The unit is in the injection process WAI TGC The unit is waiting for the ready signal from the GC ABORT The unit is aborting the run after user intervention HTA SMI Oct 2002 50 HT200H User Manual Once the sequence of injections has finished the unit will display the following message End Sequence Total I njections XX where XX is the total number of injections completed Pressing the EXI
26. ence Step NXT INS DEL END Use this command to add NXT insert INS or Delete DEL a step from the sequence Selecting insert will insert a step below the one you are editing NXT will add a step to the end of the sequence regardless of which step is being edited END will end the sequence at this step and delete any higher steps Flow Diagram of the Sequence Mode HTA SMI Oct 2002 36 HT200H User Manual Examples To run 10 samples using the same method We will assume this to be method 3 1 From the main menu press SEQ to enter the sequence mode 2 Select sequence step 1 then press the down key to change the method number 3 Use the left right arrow keys to select method 3 4 Press the down key to set the First sample Select position 0 0 5 Press the down key to the last sample Select 3 1 each row contains 4 vials starting at 0 So the sequence will run 0 0 0 1 0 2 0 3 1 0 6 Press SEQ to get back to the runtime menu 7 From the runtime menu press the down key until the Automatic Injection screen is reached Select this method by pressing the right arrow 8 At the prompt select sequence step 1 as the first sequence step Ensure that the last sequence step is also 1 9 Press START The unit will now run all 10 samples in order unless the STOP command is used Example 2 Run 10 samples using method 3 followed by 5 samples using method 6 1 Follow steps 1 5 as the above method 2
27. espective parts to their standby temperatures when the unit is switched on The temperature of the syringe can be set to between 40 170 C the temperature of the oven 40 150 C ST BY FLUSH 7 2 4 This screen allows the syringe flush function to be switched on or off when the unit is in standby Switching the flush ON will mean that the gas line will continually flush the syringe unless the unit is performing part of an injection sequence HTA SMI Oct 2002 45 HT200H User Manual The topline shows the vial size The Vial Size 20ml fixed second line shows the position of the turret tower This can be moved using Turret on the amp keys Set the standby temperature of the syringe between 40 170C The unit will heat the syringe to this temperature as soon as it is switched on ST BY OVEN Set the standby temperature of the oven between 40 150C Typically the oven temp should be lower than the syringe ST BY FLUSH Use the to switch the flush on or off INJ SYNCHRO Use the amp to switch the Injection synchronisation between normal A Start and Delayed INJ MODE Displays the mode of injection not changeable SINGLE INJECTION Method number use lt gt to select the method Sample Number use lt gt to select sample Press EXIT to return to menu Press Start to start injection Run sequence of injections AUTOMATIC RUN Press START at an HTA SMI Oct 2002 46
28. ess more than one sample at a time a method sequence must be used A sequence is a collection of individual methods The unit can store up to 16 methods in a sequence Making a sequence From the main runtime menu press the sequence button on the front panel this will enter the sequence mode A flow diagram of the sequence mode is shown on page 35 5 2 1 Sequence step The sequence step is the individual step of the sequence that you are editing You can only edit a step that has previously been created so when you create your first method the sequence step will be 1 and the left right keys will be inactive When another step has been added to the method you will be able to edit 1 or 2 5 2 2 Method Number Select the method number that you wish to use for this particular step of the sequence The individual methods have to be defined in the method mode which is explained in the next chapter The instrument can store 10 methods 5 2 3 First Sample Choose the first sample to be injected in this step of the sequence This can be anywhere on the rack independent of where previous steps may have started or finished 5 2 4 Last Sample Select the last sample to be analysed using this step This number must be equal to or higher than the first sample number If it is equal to the first sample number then only one sample will be analysed HTA SMI Oct 2002 34 HT200H User Manual 5 2 5 Sequence step This command is used t
29. et pressure does not exceed 100 kPa 1Bar 15 PSI HTA SMI Oct 2002 21 HT200H User Manual 3 6 Tray I nstallation 1 Put the plate on the support as shown bt Table 9 A Table 9 B Before installation After installation 2 Rotate it clockwise until it fits into the locking position 3 Switch the unit on and allow the unit to complete its tray identification procedure see 3 7 4 If the vial size is changed 10ml or 20ml it will be necessary to modify the Set Up parameters See chapter 4 paragraph Vial Syringe Set Up It will also be necessary to modify Vial Size and Vial Depth and perform the positioning procedure Touch amp Plunger Zero HTA SMI Oct 2002 22 HT200H User Manual 3 7 Start Up As soon as the instrument is switched on the following screen will appear represents the software version installed in the instrument HT200H Series Software rel NOTE It is possible to come back to this screen by pressing EXIT from the main menu After 5 seconds the unit will begin its setup procedure It will move the tower in the hold position and will proceed to recognize the type of tray TRAY IDENTIFYNG PLEASE WAIT It is possible that this operation will take a few minutes Pressing will interrupt this procedure and the unit will assume that it was the same tray as previously set NOTE If the unit is being used for the first time DO NOT interrupt this procedure
30. ey stops the run and changes the status to STAND BY START is used to activate preparation injection ENTER konfirms an instruction to the instrument 2 4 3 SET MET AND SEQ KEYS accesses to the configuration menu of the autosampler To get into this mode the screen must have the initial display this can be accessed by pressing ExT HT200H Series Software Rel 1 00 accesses the method setup this controls the injection parameters Finally accesses the injection sequence mode which is used to program a set of automatic injections When the unit is in the SET MET SEQ modes the respective key will be lit To exit the mode the key will need to be pressed again 2 4 4 STATUS INDICATORS On the command panel there are four lights ST BY RUN A INJ and B INJ B INJ and A INJ indicate that the sampler is in the phase before Before I NJ ection or after After I NJ ection injection RUN indicates that HT200H received the command to do one of the following actions e SINGLE INJECTION e AUTOMATIC RUN ST BY STAND BY indicates that the unit is ready to receive a command it will heat the oven and needle to the standby temperatures HTA SMI Oct 2002 15 HT200H User Manual During an automatic injection cycle ST BY on together with B INJ or A INJ indicates that the unit is waiting for confirmation to stop the run press EXIT to confirm HTA SMI Oct 2002 16 HT200H User Manual Chapter INSTAL
31. itched on or off during the incubation time of the sample Each can be set from 0 9 9minutes The unit will shake the sample vial for the ON period and leave it to settle in the OFF period It will then start again until the sample has finished its incubation If the shaker is required to be permanently ON then the Shaker ON value should be set to a non zero time and the shaker OFF value set to zero If the shaker needs to be switched OFF then the Shaker ON time should be set to zero HTA SMI Oct 2002 41 HT200H User Manual Syringe conditioning Temperature C 6 2 8 This screen marks the beginning of the syringe conditioning parameters Set the syringe temperature to the required temperature for the injection The syringe temperature is normally higher than the oven temperature The value can be set from 40 170 C SYR preFI LL FILL Volume 6 2 9 This parameter determines whether the piston is raised or lowered before the needle enters the vial If the Syringe preFill is set to YES then the piston will be up and the FILL volume will be injected into the vial before the sample is withdrawn If the preFILL is set to NO then piston will be down and the unit will not inject any air into the sample Sometimes injecting air into the vial increases the homogenisation of the sample The FILL volume is also used for the pullup strokes which are defined below Pullup Strokes Equilibrium Delay 6 2 10 The pullup strokes are the strokes
32. lemental analyser 6 Ground 7 Gas connection for washing syringe 2 1 3 OVEN SHAKER Above the keypad there are 6 injection holes ROAD f 00090000000 i Table 4 Cover of oven HTA SMI Oct 2002 7 HT200H User Manual View inside the oven There are six positions for the vials HTA SMI Oct 2002 8 HT200H User Manual 2 1 4 INSTRUMENT BASE mu Table 5A INSTRUMENT BASE with tray on the left hand side A Connectors Connector for the tray support KO 0 le eA p le Table 5B INSTRUMENT BASE with tray on the right hand side Two screws used for fixing the support for the tray 1 2 Aux Connector For connecting either an integrator or two autosamplers together The sampler can be configured with the tray on either the right or left hand side This configuration is not user changeable therefore please check that you have the correct format before proceeding HTA SMI Oct 2002 HT200H User Manual 2 1 5 VIAL RACK The sampler is supplied with a 4Ovial rotating tray This tray can accommodate either 10ml or 20ml vials Vials on the rotating plates are organised in sectors Each vial is identified with a two digit number as follows Number Number identifying the identifying the sector Position The Sector number 0 9 is written on the central part of plate The Position number is writte
33. n close to vial This number can be 0 1 2 or 3 HTA SMI Oct 2002 10 HT200H User Manual 2 1 6 SYRINGE LOCATION The syringe is located inside the tower To access to the syringe remove the screw and open the protective lid Protective lid down Protective lid up 1 Protective lid 2 Locking screw The Syringe holder is made from the following parts With Syringe Without Syringe HTA SMI Oct 2002 11 HT200H User Manual Needle guide Intermediate Needle guide Syringe location Safety lock Piston locker Syringe locker Syringe pointer Piston location CON ON U1 BR Y NH SYRINGE LOCATION SYRINGE LOCATION piston locker removed The Piston Locker has two different faces to accommodate different pistons These faces will are indicated as S side and B side S SIDE B SIDE HTA SMI Oct 2002 12 HT200H User Manual 2 2 Moving Parts There are four moving parts the rotating plate Tray TRY the tower also called Turret TUR the needle location NDL and the piston or Plunger PLG The Tray can rotate about 355 The Tower can rotate of 160 from the central position in either direction The Syringe can move vertically up to a maximum of 210mm The Piston can move up to 64mm inside the syringe Warning It is possible to move each part by hand but only with the instrument switched off Move the parts slowly and carefully Rapid movements or movements
34. nto the injector penetrating the diaphragm without an excessive bending if it does then check injector septum type of syringe used and needle tip 4 5 Vial Syringe Set Up Selecting Vial Syringe set up from the main menu enters the following screen Vial Size ml Vial Depth mm Vial Size The unit is capable of using two sizes of vials either 10 or 20ml Select the appropriate vial size using either the gt or keys Vial depth The vial depth is the position that the needle has to reach inside the sample vial This field may have values between 0mm and 25mm Use gt and to increase 9 or decrease this value in steps of 1 mm HTA SMI Oct 2002 29 HT200H User Manual NOTE Ensure that the depth set up is appropriate for the contents of the vial In particular please be sure that there is nothing to reduce the available depth such as a limited volume insert NOTE Perform the Touch Zero plunger routine after changing the vial size or the vial depth When performing the Touch Zero plunger routine after the vial size has been changed a message will appear on the screen to remind the user to either install or remove the spacers in the oven these are used to raise the height of a 10ml vial to that of a 20ml vial Syringe Size The syringe size can be either 2 5ml or 5 0ml Select the appropriate size using the left right arrow keys Syringe install The syringe installation procedure is the same
35. o its initial page and allow it to run through the start up procedure do not press EXIT when it is running the vial tray indentification WARNING Obstacle found press Exit then remove The unit has found an obstacle over the oven injector or over a vial Remove the cause of the obstruction then press START The unit will carry on from where it left off WARNING Cover jammed press Exit then fixup The cover of the oven can not open or close correctly due to a blockage Remove any object that may be blocking the oven then press EXIT to resume operations The cover is opening or closing correctly but does not give a correct signal Possible break in the transmission strap The motor does not move or turn the pulley Solution Consult your local dealer HTA SMI Oct 2002 54 HT200H User Manual Vial size mismatch Check vial on tray then START Cause Vial size not set correctly Mixed vial sizes used Solution Ensure correct vial size is installed in the software see SETUP Programming errors The following errors can happen during single injections or an Automatic Run These errors are different to the previous kind as they do not involve physical problems with the unit In each case the error message is accompanied by another line of text which indicates the position of the error in the program Sample volume gt max Met NXT Filling volume gt max
36. o move on to the Next step NXT insert INS or delete a step from the sequence To select a command highlight the cursor on the desired step and confirm by pressing the ENTER key NXT will move on to the next step in the sequence If the last step of a sequence is being edited it will create a new blank set and add it to the end of the current sequence For example if the sequence has four steps it will add a new 5 step INS inserts a new step into the sequence below the one which is being edited For example if step 3 of a 5 step sequence is being edited and INS is selected there will be a new step 3 The steps above 3 will all increase by 1 STEP STEP 1 STEP2 ____________y STEP2 STEP 3 STEP 3 STEP 4 BLANK STEP 4 STEP 5 The Effect of pressing INS when step 3 is being edited DEL Pressing DEL will delete the step that is being edited Any steps that are above the deleted steps will then decrease by one This is the opposite of INS END Pressing END finishes the sequence and deletes any step in the sequence after the current step Press EXIT return to the RunTime Menu HTA SMI Oct 2002 35 HT200H User Manual Sequence Step Select the sequence step to edit You can not add steps to the sequence with this command Method Number Set the method number that is required for this step s First Sample Choose the first sample to be injected T Last sample Choose the last sample to be injected T Sequ
37. ple Repeat 6 2 13 Injection Speed 6 2 14 Pre Injection Dwell Dwl Time 6 2 15 Copying methods Chapter 7 7 1 7 2 1 7 2 2 7 2 3 7 2 4 7 2 5 7 2 6 Run Time Menu Introduction Vial Size Turret on Stand by Syringe Temp ST BY SYR Standby Air Flush ST BY flush Injection Synchronisation Inj Synchro Inj Mode Single Injection Automatic Injection Multiple Injection 7 4 1 7 4 2 7 4 3 Set Up End Sequence Output PowerON restart Running an injection Interruption Procedure Monitoring the Injection Sequence Chapter 8 HTA SMI Oct 2002 Trouble Shooting HT200H User Manual TECHNICAL SPECIFICATIONS 1 1 Technical Specifications DIMENSIONS Height Width Length Weight With tray Electrical Voltage Frequency Power Operating conditions Ambient Temperature Humidity OPERATING SPECI FI CATI ONS Sample conditioning Oven Temperature Incubation time Progressive heating time Oven Shaking time Sample withdrawal Syringe Temperature Sample Volume Flushing flow rate Sample Homogenisation Syringe Size Injection Injection speed Pre Post injection dwell time Post injection syringe flush time HTA SMI Oct 2002 4 620 mm 400 mm 420 mm 11 5 kg 230 115V 50 60 Hz 160 VA 15 C 35 C 5 85 40 C 150 C 0 00 24 00 hr 0 00 9 59 hr Variable 40 150 C Steps of 0 01 ml 0 1 99 9 ml min Up to 15 strokes 2 5 or 5 ml 0 1 99 mi min 0 99sec 0 9 9 min
38. the four screws provided in the mounting kit 5 Open the Syringe Compartment remove the safety screw with the Allen key and open the protective lid see table 7A amp 7B 6 Rotate the tower manually and gently position over the injector Move it by pushing the upper part of the tower 7 Move the Needle Guide down over the injector by pushing on the Syringe Locker 8 Ensure the Needle Guide is perfectly centered on the injector 9 Lock the auto sampler in that position using the four screws 10 Push the Needle Guide back to the original position 11 Move the tower to the original position and close the Syringe Compartment HTA SMI Oct 2002 20 HT200H User Manual 3 5 1 Cable Connections 1 Check that the power is switched off 2 Check that the voltage is correct see paragraph 3 2 3 Connect the analyser to the 9 pin port of unit with one of the two cables provided with the instrument see table 3 4 If remote control is required connect the second cable from the RS232C port see table 3 to the serial port of the PC 5 If an integrator or second auto sampler needs to be connected the port is located under the auto sampler base see par 2 1 4 Under base area 6 Plug the power cable into the sampler 3 5 2 Gas Connection In order to flush the syringe a suitable gas source e g Nitrogen must be connected to the inlet at the rear of the unit The gas line must be regulated so that the inl
39. to be set This speed is indicated by a value between 0 and 8 see previous page Press the right arrow to select injector align and the following screen appears Injectors Alignment TURRET NEEDLE The tower will move to the location stored in memory Use the and keys to align the tower over the injector then use O0 keys to lower the syringe on to the injector This point is defined as touch point As soon as the needle touches the injector the following screen appears Injectors Alignment TURRET STO NEEDLE Screen 59A Push the key to confirm the position and store it in memory Pushing the key will exit without saving The following screen then appears Injectors Alignment STO lt mm DEPTH HTA SMI Oct 2002 28 HT200H User Manual Move the syringe holder using the and 9 keys to insert the needle into the injector at the required depth The actual depth will be displayed on the screen This value is negative as it is calculated with respect to the touch point Push the key to confirm the position and store it in memory Pushing the key will exit without saving The alignment is now complete and the screen reverts to the menu Attention Be careful in aligning the needle as it is possible to damage the syringe In particular ensure e To carefully place the needle guide on the injector e The needle guide fits properly to the injector eThe needle has to enter without impediment i
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