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DU 800 Spectrophotometer Installation and Operating Instructions

1. Analytic Wavelength 540 0 nm Bkg Wavelength 480 0 nm Bkg Correction Yes Interval Time 5 0 sec Total Time 60 0 sec Factor 1 0000 Units ng ml Temperature 37 0 Celsius DU 800 Di Nanas lt Generic User Last Blanked at 01 00 PM UY Lamp On for 1 min 26 sec a Visible UV Single Cell Holder 0 10 20 30 40 50 60 Time in Seconds Sample ID Initial Ready i Factor Corr Coeff Variance Result Time units min 1 0 0 60 0 1 0000 2 1504 0 9994 0 496597 2 1504 si Cell Match OFF 600 0nm 0 3084bs 248C 20Jul2001 12 58 PM 3 The Raw Data mode We can switch back and forth between the modes to display the acquired data or the calculated rate s or result s After we have finished the kinetic run we must decide what to do with the acquired data and results We can discard them print them and or save them In any case to complete the current run select another application or method or proceed with something else w e must execute Save and or Clear 37 DU 800 Spectrophotometer Installation amp Operating Instructions Print Save and Clear Open Save s Clear Print Print Preview Print Setup Headers Print To print the acquired data select Print from the File menu in the Applications Frame 5 Save File Save To save the acquired data select Save As from the File menu in the Applications
2. Abs 4 Color Preference MN MT IT Bold OK Cancel Help The default settings for the screen are MS Sans Serif 8 pts and for the printer Courier New 10 pts The Change Font buttons call the Windows Dialog function for the selection of fonts NOTICE A very small or large font size may not create the desirable output In this case you may consider going back to the default settings The printouts are optimized for the default settings It is important to notice that the Screen Font Characteristics are only effective for the SCAN GRAPH and the DATA GRID areas no other parts of the software are affected 84 DU 800 Spectrophotometer Installation amp Operating Instructions The of Decimal Points determine the reported precision of the Abs and T readings The defaults are four 4 for Abs and one 1 for T The Highlight Results option is unchecked by default Initially the Color Preference is set to pink and Bold is unchecked The color is selected by calling the standard Windows Dialog function for the selection of colors This option only applies to the RESULT column s in the DATA GRID areas and the printout The Default button resets the parameters of this tab Headers Tab The Header tab includes the custom header information and the selection for the length of the application header Headers apply to printouts and files Figure 54 User Options Screen
3. swswwwwo 95 Figure 65 Performance Validation Scheduler Screen swsemmmemamanmanmmmamanmnmmwaza 95 Figure 66 Performance Validation Scheduler Screen with Error Message 96 Figure 67 Restore Import System Screen ie acc dese edness eae 97 Figure 68 Add Software Options S Screen swsmmmenmanmmanmanzanzanimmnzanmnmznamnmaniaa 98 Figure 69 Select License File Sereen Sczedis ra 98 Figure 70 Add Software Options s Screen ida 99 Figure 71 Administration Menu sepais 100 Figure 72 21 CFR Part 11 Compliance 10 das 101 Figure 73 Sava Data rinso e o ld a e Beles 101 Figute 74 Data Meth dS Security it 102 Figure 75 Data Directory Structure tin A A ta 103 Figure 76 Data Directory Structure isis 104 Figure 77 Headers for current TU ir 105 Figure 78 Enhanced ai ese aa e iaa e EE lal Siar tA ede 105 Figure 79 System Status SOTE sisisi manii ait iiai eiin 107 Figure 80 Wavelength Calibration Screen cuac its di ida 107 Figur 81 Baseline Test Screen a ees EEE 108 Fig re 82 Set Scan Gains Sereen ii Be wi i eee 109 Figure 83 Reset Source HOUSE 110 viii DU 800 Spectrophotometer Installation amp Operating Instructions Figure 84 Service Diagnostic 111 Figure 85 Calibration Information Screen ecceescecesscecesececeeeeeceeceecnseeecsseeeesteeeenaeees 111 Figure 86 Wavelength Drive Run Screen wwwmmmwemanemanmmwamwamm aim anena 112
4. 901289L Al WARNING For continued protection against risk of fire replace the fuses only with the type and current rating specified above ATTENTION Afin d assurer unne protection permanente contre les risques d incendie remplacer uniquepment par un fusible de meme type et valeur Insert the fuse compartment and press to secure Plug the power cord back into the instrument Turn the instrument around so that it is facing forward RN A Turn the instrument ON 131 DU 800 Spectrophotometer Installation amp Operating Instructions UV Source Replacement Parts Required UV Source P N 514366 1 Turn the instrument off unplug the power cord and allow the instrument to cool down for 15 minutes CAUTION To avoid risk of electrical shock disconnect power cord to instrument before changing either source Attention Risque de choc electrique Debrancher l appareil avant de changer de source The UV lamp operates at a high temperature To prevent burns allow at least 15 minutes for the instrument and sources to cool before handling internal components The UV lamp generates UV light Do not look directly at an operating lamp without wearing proper UV protective eye glasses 2 Locate the source access door on the top left hand of the instrument cover then loosen the thumb screw and open it 132 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 99 Location
5. Application DNA Ratio 260 285 Philip Pappas Fixed Wavelength Scans for 4302 Ron Steiner Wavelength Scan Select All Copy From User Close Help The lt Default Method gt s are fixed methods and are not listed When identical method names are encountered a sequential number lt method name gt 01 is appended to the copied method name Copy to Custom Applications A method can become a custom applications by copying it to Custom Applications This menu item is disabled for a lt Generic User gt but enabled when a user or 63 DU 800 Spectrophotometer Installation amp Operating Instructions administrator is logged on Only methods from the current user or the administrator are shown and can be copied The lt Default Method gt is a fixed method and is not listed Figure 39 Copy Methods to Custom Applications Screen Copy Methods to Custom Applications x Select All Copy To Custom One or more methods may be selected and copied by clicking on the Copy To Custom button The button is enabled when one or more items are selected When identical method names are encountered a sequential number lt method name gt 01 will be appended to the copied method name A custom application has an association with the application and is protected from common method editing Custom applications are publicly available applications and can only be deleted by the administrator 64 DU 800 Spectro
6. fla DU 800 U is Spectrophotometer DNA Group USBO01 E f xj System Applications Methods Tools Diagnostic Help Kinetics Time y T 60 Read Ma RediRead fil Cell Match G OpenFile w Accessories D lt Default Method gt wa a YA RediScan Clear LA Save File Edit Method Method Parameter Value File Mode Function Axis Help Data Acquisition Sampling Device Holder Holder Type Single Cell Number of Cells 1 Sample Assignment Analytic Wavelength 540 0 nm Bkg Wavelength 480 0 nm Bkg Correction Yes Interval Time 5 0 sec Total Time 60 0 sec Factor 1 0000 Units ng ml Temperature 37 0 Celsius DU 800 O aun A 0 10 20 30 40 50 60 COULTER nas Time in Seconds lt Generic User i BLANK REQUIRED i ada Time sec bm Visible UV Single Cell Holder Ready CellMatchOFF 6000nm O 308Abs 248C 20Jul2001 1258PM The following three things have changed when we closed the Method window First the Method Parameter List has been updated Notice that it display the parameters we have changed earlier in the method Second the dAbs min column has been added to the data grid for each sample if we would have multiple samples We are now ready to start the kinetics run using the customized parameters But before we do that we need to blank the system on the selected wavelengths the analytical and the background wavelength Blanking A blank is required when lamps are turned on or certain parameters change
7. 100 DU 800 Spectrophotometer Installation amp Operating Instructions With the feature activated RediRead and RediScan become inaccessible and cannot be used to take measurements Figure 72 21 CFR Part 11 Compliance xl Data Directory Structure Data Methods Secunty 21 CFR Part 11 Compliance Enhancements Lock RedRead and RediScan Force Saving of Data Force Users to Save Data This feature allows the administrator to force users to save the acquired data With this feature activated users cannot take measurements and then discard them The acquired data must be saved to the specified location before exiting the application The check box for this feature is located in the 21 CFR Part 11 Compliance tab of the Security Enhancements item in the Administration menu By default the parameter Force Saving of Data is unchecked When checked a the Save dialog box cannot be cancelled and b the directory cannot be chosen the default directories are used and the destination tree is displayed in the dialog box Figure 73 Sava Data x C DU800 Data Fixed Wavelength Vitamin Ron Steiner Filename Save As Type Read Data dux wa 101 DU 800 Spectrophotometer Installation amp Operating Instructions Data Methods Security The window with the Data Methods Security tab can be accessed from the Administration menu Figure 74 Data Methods Security x
8. 81 DU 800 Spectrophotometer Installation amp Operating Instructions NOTICE Be aware that the Administrator and the User logon of the DU 800 are entirely independent of the Microsoft Windows Operating System logon System Options In general System Options apply to the lt Generic User gt and the Administrator Many of these parameters serve also as default preferences for Users Setup Tab The Setup tab determines the path of the user directory In addition it allows the administrator to determine timeout functions This tab does not exist in the User Options Figure 51 System Options Screen with Setup Tab Selected Setup Output Headers Graph Colors Regulatory Administrator Default Paul Novak Lab 101 Data Root C xDU800 Data FA Idle Timeout Functions T Automatic Logoff Elapsed Time 10 minutes T UV Lamp Shutoff Elapsed Time 2 0 hours T Visible Lamp Shutoff Elapsed Time 20 hours x OK Cancel Help The information in the Administrator field will be used in message boxes that refer to the administrator We recommend to enter the administrator s name With an empty field the text Administrator will be used Data files will be stored and accessed in the DATA Root which is easily accessible through the Windows Explorer The default directory is C DU800 Data and may be changed by the administrator Instead of a fixed path a UNC Universal Naming Conv
9. Performance Yalidation Scheduler X A The following validation program s have been i performed successfully Wavelength Drive Test Resolution Test Baseline Flatness Test Noise Test Press OK to continue 95 DU 800 Spectrophotometer Installation amp Operating Instructions With one or more specifications outside of the given limits the following message appears Figure 66 Performance Validation Scheduler Screen with Error Message Performance Yalidation Scheduler x Q The following validation program s have been performed Wavelength Drive Test Resolution Test Baseline Flatness Test Noise Test One or more parameters are outside of the accepted specifications Please inform the administrator Paul Novak Lab 101 The use of the instrument is currently not recommended The Performance Validation programs are described in Applications Software Performance Validation The Default button resets the preferences of the Performance Validation Scheduler Backup Export System The function will be used by the administrator to Backup Export System parameters The reason for this is the safekeeping of system information in a file to restore the entire system after an system failure or when the DU 800 System and Applications Software must be reinstalled The service representative may also use this function to backup the entire system and restore it after the service call First the user selects a ba
10. 248C 10Now2000 11 204M y Ready After we have taken the readings of all samples we must decide what to do with the acquired data We can discard them print them and or save them In any case to complete the current run select another application or method or proceed with something else we must execute Save and or Clear Print Save and Clear File Open Save s Clear Print Print Preview Print Setup Headers Print To print the acquired data select Print from the File menu in the Applications Frame 24 DU 800 Spectrophotometer Installation amp Operating Instructions 5 Save File Save To save the acquired data select Save As from the File menu in the Applications Frame or click on the Save File icon button The Save File dialog appears which lets you enter an appropriate filename Click on the OK button to save the data or Cancel to abort If you don t change the target directory the data file DUX is saved to the following location C DU800 Data Generic User Fixed Wavelength lt Filename gt dux if the administrator has not changed the default DATA root directory previously E Clear Clear To end the current task and reset the application e g for another task or to leave the application select Clear from the File menu in the Applications Frame or click on the Clear icon button A message will inform you if the data has not been saved The dat
11. COULTER DU 800 Spectrophotometer Installation and Operating Instructions 512860AE November 2009 Beckman Coulter Inc 250 S Kraemer Blvd Brea CA 92821 2009 Beckman Coulter Inc DU 800 Spectrophotometer Installation amp Operating Instructions Table of Contents OOOO O O O o OVVIE Wei A a te E AA a AA 1 Standard Applications Software sco i iscc essa oes idas 2 Optional Applications Software eecccesscecssccecesececeseceseececesececseacecnsecesssecessseeeees 2 Principles of Operation a A A ES 4 Optical Principle tandas 4 BLOOD 4 BR AGN SI WI IA nals O 5 SCARING io 6 Read Average Time ii 6 PTO OUTS O 6 Spectrophoto medad 7 LES aaa BI AA YA AE AAA AAA AA 8 SoftWare AA 8 space RU AA 9 POWER waw 10 Unpacking Ta KAA 11 Shipping Kind 12 PC Monitor and Optional Panter i543 sisada eet wien ea eta oem mee 12 Hardware Installation tiros 13 Software TSP ALIA ON ai 15 General AA Un Yara aw 17 Taking Single Wavelength Readings ni a 18 Editing a Med Iii 19 BIA i 22 RE e cateo re lacas e 23 Print Save ani ai aa Site Bag eMac 24 TA CAN rence Aaa 25 TSG MIN ea Method teria AA 26 BIA aiding Neer KA LS 28 A Wa 28 Print Save and Cleat 2 A A Ad AA Ai 30 P rtormins a KINGO iii 30 Editing MEMO a 31 Blank sil ti rd jas 34 DU 800 Spectrophotometer Installation amp Operating Instructions IR GALEN O eds 35 Print Save and Cl awa 38 AA A A O Miata 39 DANDE ld ii a Edd 39 Pile Extensions irdan
12. Comment This is a note Product Name Abuprofin Lot de poy 1234 Read Mode Abs Calculation Mode Formula Number of Wavelengths 2 Wavelength we 2500 2 30 0 0 Formula wl350 wl400 0 x 1000 Unit ng ml 250 0 nm 300 0 nm Results Sample 1D Abs Abs ng ml My Sample ID 1 0 0374 0 0323 12 4939 Sample 2 1 0 0374 0 0323 9 4832 Electronic Signatures 21 CFR Part 11 By definition data and result files DUX that are created by the DU 800 Spectrophotometer are electronic records Title 21 Code of Federal Regulations 21 CFR Part 11 Electronic Records Electronic Signatures came into effect on 20 August 1997 and sets forth criteria under which the agency considers electronic records electronic signatures and handwritten signatures executed to electronic records to be trustworthy reliable and generally equivalent to paper records and handwritten signatures executed on paper People using electronic signatures must certify to the agency that the electronic signatures in their system are intended to be the legally binding equivalent of traditional handwritten signatures For more information please refer to Code of Federal Regulations Title 21 Part 11 Electronic Records Electronic Signatures U S Government Printing Office http www access gpo gov nara cfr cfr retrieve html page CITE 21CFR11 1 View Electronic Signature s This item is
13. Performance Validation Scheduler Backup Export System Restore Import System Add Software Option s Create Backup License File RediRead Pia Redi Read RediRead provides an easy and fast way to take readings at a fixed wavelength and provide a printed output In this mode the system will not store data or perform calculations RediRead acts like an interrupt program that keeps the workflow of the current application intact The RediRead window is used to take single wavelength readings on one or more samples The cell position of a multicell holder cannot be changed from within the program 68 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 42 RediRead Screen RediRead X Commands Help Wavelength nm 480 0 Parameters ae Abs 480 0 nm Com 0 3324 Abs Wavelenath 480 0 nm 0 3117 Abs 480 0 nm 55 0 T 480 0 nm 53 2 T 480 0 nm 0 1869 Abs 480 0 nm 0 1906 Abs Reading 0 3324 Abs BLANKED The read average time is fixed at 0 5 seconds The user simply selects the appropriate read mode Abs or T and wavelength Before taking a measurement RediRead needs to be blanked on air or a blank solution This is done by clicking on the Blank menu item or button When a blank has been performed the indicator BLANK REQUIRED will change to BLANKED and the user is ready to take measurements NOTICE Because th
14. fla DU 800 UV Vis Spectrophotometer DNA Group USB001 of x System Applications Methods Tools Diagnostic Help eu gt Ss F RediRead ff CellMatch gt OpenFile 1 Accessories D can al F RediScan I Clear Ged Save File Edit Method A Method Parameter Value File Mode Function Axis Help Scanning Sampling Device Holder Holder Type Single Cell Number of Cells 1 Scan Mode Abs Start Wavelength 500 0 nm End Wavelength 800 0 nm Wavelength Interval 2 0 nm Scan Speed 2400 mm min Auto Print No Auto Save No Filename Pretix Default DU 800 UW Vette Seci lt Generic User gt Last Blanked at 12 04 PM 500 550 600 650 700 750 800 UV Lamp On for 53 sec Wavelength in nm a Visible UV 1 1 M Sample 1 1 2 1 M Sample 2 1 Single Cell Holder 3 1 M Sample 3 1 Tr isi Ready Cell Match OFF 800 0nm O 308Abs 248C 13Nov2000 11 594M yz After we have taken the scans of all samples we must decide what to do with the acquired scans We can discard them print them and or save them In any case to complete the current run select another application or method or proceed with something else we must execute Save and or Clear 29 DU 800 Spectrophotometer Installation amp Operating Instructions Print Save and Clear Open Save Highlighted Scan Save Marked Scans Save Mode File Clear Print Print Preview Pri
15. Analytical Wavelength 540 0 nm Background Wavelength 490 0 nm IV Background Correction m Kinetic m Curve Offset Samples a None Interval Time 50 Seconds y a bata Total Time e0 Seconds y z Factor Units 1 0000 ng ml Temperature 370 dE M Delta Abs Save As OK Cancel Help In this example we will use the modified default parameters temporarily To do so we confirm the changes by clicking on the OK button In this case the selected parameters remain only valid until you leave the Kinetics Time application or select a new method You may save the modified default method parameters permanently under an appropriate method name by clicking on the Save As button In this case the next time you use the Kinetics Time application just select your saved method from the Method menu or the method drop down list box and you are ready to go using your individual parameters You may change your method parameters at a later time or save the method under a different name We have now set up the Kinetics Time application in a way to fit our needs by modifying the method in this case the lt Default Method gt and your window should now look like the one shown below 33 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 25 Window with Kinetics Time Application and All Changes Made
16. amp Operating Instructions In order to receive accurate results it is recommended that both lamps are warmed up UV lamp requires one hour prior to running Performance Validation tests If either lamp is off at the scheduled time the Performance Validation Scheduler will turn on both lamps and wait one hour before executing the selected test s The Source Scheduler can be used to automatically turn on the lamps one hour before the Start Time selected in the Performance Validation Scheduler and to turn off the lamps at appropriate time e g at the end of a work day After the selected Performance Validation program s have been completed the results may be printed and saved automatically By default Print and Save are checked If Save is checked the results will be saved to a file The filename is based on the Results File Prefix The date of the test s will be appended to the filename prefix automatically in the following format lt file prefix gt YYMMDDHHMMSS txt The location for the file is the Performance Validation directory in the DATA root directory When the Performance Validation Scheduler activates the following message is displayed Figure 64 Performance Validation Schedules Activation Dialog xi i Performance Yalidation test s in progress Estimated Completion Time is 4 35PM When all tests are within specifications the following message appears Figure 65 Performance Validation Scheduler Screen
17. amp Operating Instructions Manual Advance Button This button is located on the back of the batch sampler and will raise the aspirator arm if it is lowered and advance the racks one sample position Manual Movement of the Aspirator Arm The aspirator arm can be moved manually up and down to and from the wash station if installed and to the next tube position To move it click on the respective button in the Batch Sampler tab Automatic Operation 1 10 If the batch sampler aspirator tube is positioned above the last position in the red stop rack press the Manual Advance button on the back of the batch sampler to advance the racks one sample position Clean the flow cell by flushing with distilled water then an alkaline solution distilled water again and finally the solvent used for the analysis Several seconds with each solution should be sufficient for cleaning if the flow cell was cleaned after the previous use Empty the flow cell by pressing FLUSH for about 5 seconds Make sure that Batch Sampler is selected in the Sampler tab of the Method window Place all solution in 13 x 100 mm test tubes Blank Place the blank directly under the aspirator tube Click on the Lower Arm button to lower the aspirator arm Press FLUSH until the blank solution is aspirated into the flow cell and blank the system Then press FLUSH to aspirate the solution to waste Raise the aspirator arm by clicking on the Rais
18. will prompt the user to specify a new filename When a file is saved by a user with Signature Privilege the Add Electronic Signature dialog is displayed 47 DU 800 Spectrophotometer Installation amp Operating Instructions Method Parameters Frame Accessories WA Accessories Opens the Accessories window and allows the user to setup the accessories for the system This icon button is a shortcut for the Accessories item in the System menu Method Parameter Sampling Device Holder Type Number of Cells Read Mode Calculation Mode of Wavelengths Wavelength 1 Wavelenath 2 Wavelenath 3 Read Average Time This part of the front end provides a listing of the major method parameters With an open file the file parameters are displayed and the column header changes to File Parameter The data in the grid are for information only and cannot be edited 48 DU 800 Spectrophotometer Installation amp Operating Instructions Status amp Control Frame This part of the front end combines important Sacco DU 800 status information with the control of key e ES aiic spectrophotometer elements such as sources lt Generic User and sampling device Last Blanked at 09 38 AM UY Lamp On for 43 sec a Visible UV 1123 45 56 HOME Status Information User Status The current user is displayed in the User Status label When no user is logged
19. 21 CFR Part 11 Compliance Enhancements Data Directory Structure Data Methods Security 1 Disallow Citation of Methods by User TF Show Only Custom Applications Disallow Creation of Methods by User This feature allows the administration to set up the system so users cannot create their own methods and use them to acguire data and create records In this case only administrators will be able to create methods Thus limited users to methods that have been verified by the company This setting will have no affect on administrators The check box for this feature is located in the Data Methods Security tab of the Security Enhancements item in the Administration menu By default the parameter Disallow Creation of Methods by User is unchecked When checked the menu item Create Edit Method and the shortcut icon Edit Method will be disabled for all users In addition the following menu items will not be accessible with the feature activated Create Edit Method Delete Current Method Copy Method from Other User Copy Method to Custom Applications Backup Export Methods Restore Import Methods Show Only Custom Applications This feature works best in combination with the feature Disallow Creation of Methods by User With both features selected the administrator can set up the system in a way that a user can only select and apply Custom Applications Thus giving the company compete control over h
20. 77 Figure 49 Electronic Signature Authentication Screen cece eeseceseceseceeeeeesecsseeeseeeenees 79 Figure 50 Add Electronic Signature Screen ooococcconcncnnnnonncnononnnnnono non nnnnnnnnn nr non nro ncnnn canos 80 Figure 51 System Options Screen with Setup Tab Selected ooooonoccninncnonccinccnocnnonnnannnnnnoss 82 Figure 52 User Authentication Screen q iia 83 Figure 53 User Options Screen with Output Tab Selected wwmmmmmmmmmwa 84 Figure 54 User Options Screen with Headers Tab Selected ww mmmmommmmaza 85 Figure 55 User Options Screen with Graph Tab Selected eee eeeeeseesneceseeeseeeenees 86 Figure 56 User Options Screen with Colors Tab Selected w wmmmmmmmnmmmaza 87 Figure 57 System Options Screen with Regulatory Tab Selected o 88 Figure 58 Password Renewal Screen eescesesseeeseecsseceseeeseecaeceseesseeseseecaeesseessneeenees 89 Figure 59 User Account Manager Screen var ii 90 Figure 60 Delete Custom Applications Screen oocoonoocccconcccnoncnononcnononaconncnonnncncnnnccnnnncnnnnnos 91 Figure 61 System Audit Trail Screen A A A tt 92 Figure 62 Source Scheduler Screen of cies ji cose caecnea staged ssaacesscantevaeassinaccosaosteeclaacesaeerens 93 Figure 63 Performance Validation Scheduler Screen eee eeceeseeeseceeeeeeeseseceseeeseeeenees 94 Figure 64 Performance Validation Schedules Activation Dialog
21. 800 Spectrophotometer Installation amp Operating Instructions Batch Sampler Accessory The batch sampler is an autosampler capable of holding multiple samples in test tubes The solutions held in the test tubes are aspirated into the sipper flow cell for analysis using the sipper pump After the analysis the samples either are returned to the batch sampler or are sent to waste If the batch sampler has a wash station the flow cell can be rinsed between samples Figure 94 Batch Sampler Accessroy The batch sampler offered by Beckman Coulter is capable of holding up to 114 samples Currently this is the only batch sampler that is supported by the software NOTICE The sipper accessory is required The batch sampler must be selected in the Batch Sampler tab of the Accessories window for proper operation The fill time return time read delay time and the sipper speed parameters in the same tab might be optimized for the samples The recommended minimum sample volume with the batch sampler is 1 5 mL Refer also to the Installation Instructions Batch Sampler Accessory P N 512871 Beckman Coulter ISCO Batch Sampler Stop Rack The batch sampler is provided with 18 white test tube racks and 1 red stop rack The stop rack is sensed by the batch sampler When the last sample position of the stop rack is reached the analysis will stop even if there are more samples to read 121 DU 800 Spectrophotometer Installation
22. Change User for more information On Line Launch with System Initialization The DU 800 Spectrophotometer is initialized by the Windows software when it is detected on the USB port It is recommended to turn the instrument on before launching the software NOTICE Lift the sample compartment cover of the DU 800 Spectrophotometer and verify that there is nothing in the sample compartment that can block the light beam After turning on the DU 800 Spectrophotometer for the first time wait approximately 7 seconds before starting the software Failing to do so may result in the message Another program is already running or the system just sits there without initializing In this case close the software turn the spectrophotometer OFF wait a couple of seconds and then turn it ON again After waiting for approximately 7 seconds start the software 41 DU 800 Spectrophotometer Installation amp Operating Instructions When an instrument is detected on the USB port the software downloads the firmware to the DU 800 and builds the wavelength calibration table This takes approximately 3 minutes Then various Startup Diagnostic Tests are performed and the result of each test is reported The time for each of these tests varies Please refer to Troubleshooting Startup Diagnostic Tests should one or multiple tests fail During the initialization process the window below displays the progress and allows the user to take a
23. DU 800 Spectrophotometer Installation amp Operating Instructions Figure 15 Method Window for Fixed Wavelength After the Change Method for Fixed Wavelength x lt Default Method gt Application Sampler Report Info m Read Mode Calculation Mode Default Absorbance None Transmittance C Factor C Formula Number of Wavelengths 1 Wavelength 260 0 Save As OK Cancel Help In this example we will use the modified default parameters temporarily To do so we confirm the changes by clicking on the OK button In this case the selected parameters remain only valid until you leave the Fixed Wavelength application or select a new method You may save the modified default method parameters permanently under an appropriate method name by clicking on the Save As button In this case the next time you use the Fixed Wavelength application just select your saved method from the Method menu or the method drop down list box and you are ready to go using your individual parameters You may change your method parameters at a later time or save the method under a different name We have now set up the Fixed Wavelength application in a way to fit our needs by modifying the method in this case the lt Default Method gt and your window should now look like the one shown below 21 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 16 Window with F
24. Enhancements tab can be accessed from the Administration menu Figure 78 Enhanements ES uU x Data Directory Structure Data Methods Security 21 CFR Part 11 Complance Enhancements Automatically Convert to CVS Fie Automatically Convert to CSV File This feature allows the administration to set up the system so users CSV Comma Separated Values files are created automatically without manually executing the Data Export function each time This setting affects all users and administrators By default the parameter Automatically Convert to CSV file is unchecked When checked the Data Export item in the Tools menu will be disabled since the function is no longer needed 105 DU 800 Spectrophotometer Installation amp Operating Instructions With the feature activated the CSV file will be created automatically when the 21 CFR Part 11 compliant DUX file is created The CSV file will have the same filename as the DUX file which is specified by the user when the data is saved Diagnostic Menu Diagnostic System Status User Diagnostics Calibrate Wavelengths Run Baseline Test Set Scan Gain Reset Source Hours Reset System Service Diagnostics The Diagnostic functions consist of two parts The System Status and the User Diagnostics windows are accessible by any user and are described below The Service Diagnostics functions are reserved for service personnel and c
25. Park London Road High Wycombe Buckinghamshire HP11 1JU England Phone 44 1494 441181 FAX 44 1494 447558 E Mail beckmancoulter_uk beckman com 148 DU 800 Spectrophotometer Installation amp Operating Instructions Asia Pacific Australia Beckman Coulter Australia Pty Ltd 24 College Street Gladesville NSW 2111 Phone 61 2 9844 6000 Phone 1 800 060 880 FAX 61 2 9844 6096 Sales amp Marketing FAX 61 2 9844 6098 Service China Beckman Coulter Inc Beijing Representative Office Unit 2005A 2006 2009 East Ocean Center 24 Jian Guo Men Wai Avenue Beijing 100004 Phone 86 10 65 15 6028 FAX 86 10 65 15 6025 Hong Kong Beckman Coulter Hong Kong Ltd 12th Floor Oxford House 979 King s Road TaiKoo Place Phone 852 2814 7431 Phone 852 2814 0481 FAX 852 2814 1599 FAX 852 2873 4511 E Mail beckmancoulter_hk beckman com Japan Beckman Coulter K K Toranomon 37 Mori Building 3 5 1 Toranomon Minato ku Tokyo 105 0001 Phone 81 3 5404 8359 FAX 81 3 5404 8436 149 DU 800 Spectrophotometer Installation amp Operating Instructions Singapore Beckman Coulter Singapore Pte Ltd 116 Changi Road Unit 03 01 02 Singapore 419718 Phone 65 339 3633 FAX 65 336 6303 Taiwan Beckman Coulter Taiwan Inc Taiwan Branch 8th Floor 216 Tun Hwa South Road Section 2 Taipei 106 Taiwan Phone 886 2 2378 3456 Phone 886 2 2378 8000 FAX 886 2 2377 0
26. READ button to start the kinetic run As determined in the method this will take approximately 60 seconds During the run the absorbances and the calculated rates for each time point dAbs min are displayed in the data grid You should also see the net absorbances in the graph window After all readings have been taken within the run the Rates item in the Mode menu will be enabled You now have three modes available and the windows should look like the ones below 35 DU 800 Spectrophotometer Installation amp Operating Instructions 1 The Data Acquisition mode which we have just completed Figure 26 Data Acquisition Mode fla DU 800 UY Vis Spectrophotometer DNA Group USBO01 Applications Methods 3 lt Default Method gt 4 4 Method Parameter_ Value 36 DU 800 Spectrophotometer Installation amp Operating Instructions Ze The Rates mode which shows the rate s or result s Figure 27 Rates Mode fla DU 800 UY Yis Spectrophotometer DNA Group USBOD1 15 x System Applications Methods Tools Diagnostic Help aux 66 eee MA RediRead fill Cell Match G OpenFile w Accessories So ea z Fh RediScan lle Clear E Save File Edit Method SA File Mode Function Axis Help Kinetics T ime y Default Method Method Parameter Value Sampling Device Holder Holder Type Single Cell Number of Cells Sample Assignment
27. TEMPERATURE USB SIPPER BATCH CONTROLLER P gt on uu O SSCS o e 2 901225L Al 2 Attach the power cord compatible with your country s voltage outlet to the back of the DU 800 and plug the other end into a grounded electrical outlet The instrument contains a switching power supply that covers the range from 100 240 VAC NOTICE Do not power on the DU 800 at this time 3 If purchased the installation of the Sipper Accessory Batch Sampler and or Peltier Temperature Controller should be performed now Follow the installation instructions supplied with each accessory 13 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 10 Place the PC in a convenient location near the DU 800 and attach the flat connector of the USB cable to the USB port of the PC Attach the other end of the connector to the USB port located on the back of the DU 800 USB Connections on the PC and Spectrophotometer COMPUTER SPECTROPHOTOMETER e A 73 4 i pa d WA UM oe ey O o 38 z 89 88 98 88 0 gt 96 Q0 26 901226L Al NOTICE It is recommended that you label the USB Port on the PC as DU 800 and always use this port for the connection to the Spectrophotometer Switching the cable to a different port after the DU 800 software is installed will require a manual installation of the drivers Setup the PC with monitor and peripherals Make sure that it meets th
28. adjusted each time that the wavelength drive is calibrated as described earlier When a wavelength scan is performed using a blank that has significant absorbance in the 108 DU 800 Spectrophotometer Installation amp Operating Instructions scanning range it may be desirable to change the gain settings in the instrument to compensate for the absorbance of the blank This will increase the dynamic range of the instrument when scanning using the absorbance blank Figure 82 Set Scan Gains Screen Set Scan Gains X 1 Turn UY and Visible lamps ON 2 Remove any samples and blanks from the sample compartment for most scanning applications For scanning with highly absorbing blank solutions place the blank solution in the sample compartment 3 Close sample compartment cover WARNING If you use Set Scan Gain with as blank solution you must return to this screen and set the scan gain without a blank This will restore the gains to the normal settings Help Set Scan Gains Now Close To change the scan gains follow these steps 1 Verify that both sources are turned on and that they have stabilized for at least one hour 2 Place a cuvette containing blank solution in the cell holder Click on the Set Scan Gains Now button The instrument adjusts the scan gain values to compensate for the absorbance of the blank NOTICE The instrument will continue to use the new gain values until the gain is adjuste
29. and a UV Lamp Deuterium Halogen The visible lamp covers the range from 321 to 1100 nm while the UV lamp provides the energy for the wavelength range from 190 to 415 nm For the following examples both lamps should be turned on Click on the Visible icon button to turn on the visible lamp and then click on the UV icon button Visible 2 UV 17 DU 800 Spectrophotometer Installation amp Operating Instructions The visible lamp is turned on immediately while the UV lamp requires approximately 30 seconds warm up time before it can be used The warm up period is indicated by the status message Warming up UV lamp while the UV icon button flashes When a lamp is turned on the respective menu item is checked and the caption of the respective icon button turns red After the UV lamp has been warmed up the message in the Status amp Control Frame reads UV Lamp On for x sec the unit can be seconds minutes or hours This provides additional information for the user in regard to the UV lamp warm up time This sections provides step by step operating instructions for three simple standard applications Taking Single Wavelength Readings Taking a Wavelength Scan Performing a Kinetic Run Taking Single Wavelength Readings All applications except Wavelength Scan I and II take one or multiple single wavelength readings in one form or another e g reading at 260 0 nm and 280 0 nm Make sure that the Fixed Wavelength
30. application is selected and that the current method is the lt Default Method gt by checking the drop down list boxes in the Toolbar If not click on the down arrow of the drop down list box and select the appropriate item You should see a window similar to the one shown below 18 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 13 Window with Fixed Wavelength Application Selected fla DU 800 UY Vis Spectrophotometer DNA Group USBO01 10 x System Applications Methods Tools Diagnostic Help Fixed Wavelength Em Lo Read Ya Redi Read fill Cell Match gt Open File mf Accessories D lt Default Method gt Biska Fh RediScan Clear bed Save File Edit Method Method Parameter Value File Help Sampling Device Holder Holder Type Single Cell Number of Cells 1 Read Mode Abs Abs 200 0 nm Abs 250 0 nm Abs 300 0 nm Calculation Mode None of Wavelenaths 3 Wavelength 1 200 0 nm Wavelength 2 250 0 nm Wavelength 3 300 0 nm Read Average Time 0 5 sec Abs Abs Abs Sample ID 200 0 nm 250 0nm 300 0 nm DU 800 A UV lt Generic User gt BLANK REQUIRED UY Lamp On for 11 min 3 sec Visible UV Single Cell Holder sj Ready Cell Match OFF 600 0nm 0 3084bs 248C 10Nov2000 10 35AM y You are now ready to take single wavelength readings at three wavelengths 200 0 250
31. applications and are described in Toolbar and Icons Main Menu The top menu provides access to system wide functions Method Parameters Frame This list box displays relevant method parameters at all times Status amp Control Frame Displays the status of the system User Logon Blank Status UV Lamp Status and provides common control functions Toolbar and Icons The toolbar at the top of the screen just below the main menu provides quick access to key elements related to the operation of the system and application Applications Frame The applications frame holds the applications menu bar all elements related to the selected application and displays the acquired data Permanent Status Bar The status bar at the bottom displays system parameters and messages Main Menu The main menu provides access to all functions System Applications Methods Toole Diagnostic Help 44 DU 800 Spectrophotometer Installation amp Operating Instructions During an open run functions in the Applications and Methods items are disabled They will be enabled again when the user executes the Clear command which closes the run To switch to another application or select another method the user must first close any open run When the instrument is busy measuring samples functions that may interfere are disabled temporarily Toolbar and Icons The toolbar is fixed and cannot be removed by the user It provides shortcu
32. by scanning them versus air before using them in the UV region To re zero the instrument at any time between samples insert the same blank solution and click on the BLANK icon button The instrument stores the blank and uses it until either the sources are turned off another blank reading is taken or certain parameters are changed For best results the instrument should be blanked frequently allowing the blank reading to be taken shortly before the sample measurement is taken A new blank should be read if the instrument has not been used for an hour Reading The background reading taken as part of the Blank procedure is stored in the instrument and can be reused for an unlimited number of sample readings as long as the wavelengths remain the same When a new blank is required it is indicated in the Status amp Control Frame A new blank reading should be taken every time a solvent is changed because the blank reading will likely be different A new blank reading should also be made if no reading has been taken for over an hour To blank click on the BLANK icon button while in the appropriate application For multiple wavelengths up to 12 a blank reading is taken and stored for each selected wavelength DU 800 Spectrophotometer Installation amp Operating Instructions As the sample data are collected the blank is subtracted and the difference in absorbance or transmittance is reported Scanning The backgrou
33. enabled when a file has been opened and when an electronic signature exists in the file The function calls a window that displays the Signer s Name and Role Author Reviewer or Approver the Date Time of the signature and a Note or Annotation if one exists All signatures included in the file will be displayed A file may contain up to five signatures 78 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 49 Electronic Signature Authentication Screen Electronic Signature Add Electronic Signature The Electronic Signature consists of encrypted information based on the user name and password as well as the serial number of the instrument In addition to the signature the file also includes Name of the Signer in readable form Date and Time of the Signature Role of Signer Author Reviewer or Approver Note empty by default 79 DU 800 Spectrophotometer Installation amp Operating Instructions Once added the Electronic Signature will become a fixed part of the DUX file Figure 50 Add Electronic Signature Screen Add Signature test dux y x oO Do you Administrator want to sign this electronic record Signature 2 of 5 Author Reviewer C Approver Note w uo The Add Electronic Signature menu item will give a logged on user or the administrator the ability to sign a file The menu item is only enabled when a file
34. for one reason or another the user will be alerted by the message Communication with the spectrophotometer has been lost Please check the connection The software re establishes communication with the spectrophotometer automatically when the connection is restored Off Line Launch When no instrument is detected on the USB port the software is launched in off line mode This would be the case when the software has been installed on another PC for post run analysis or other purposes The fact that the software is running in off line mode is indicated in the software caption Common Front End User Interface The DU 800 System and Applications Software integrates all system functionality and the various applications in a common user interface front end This allows the user to operate all aspects of the system from a single vantage point The integrated user interface includes key control elements common to all applications The front end and other system related functions are described in the System Software section The standard applications are available with the DU 800 System and Applications Software installation Standard and optional applications are described in the Applications Software section Optional applications or software options can be added via a license contained on a floppy disk The addition or installation of software options is described in Add Software Options The desired application is selected from the ap
35. has been opened and if the user has a Signature Privilege which is assigned by the Administrator in the User Account Manager screen Before a signature can be added the user will be prompted to repeat the logon The signature is added to the existing file immediately The file does not need to be saved to add the signature Existing DUX files cannot be overwritten Trying to save a file under the same name will prompt the user to specify a new filename If the user saves data under a new filename all existing signatures will be removed A user or the administrator may sign a file as an Author Reviewer or Approver depending on the available signature privilege Only one Author is allowed per file If an Author already exists the Author button will be disabled Multiple Reviewers and Approvers are permitted When attempting to add a signature twice e g same user in a different role the user will be prompted A file can include a maximum of five signatures The Note field allows the user to add an annotation User Options This item is only visible when a user is logged on and disabled with a lt Generic User gt and the administrator The function calls a window and lets the user set his her preferences Many user parameters are identical to the system parameters The default settings for user parameters are the system parameters User preferences have priority over system parameters and are used in place of the system parameters The
36. igure 87 DRF Scan Screen Git Aca et or case A eee Aes nl e rea dls o 113 Figure 88 Blocked Beam Noise Screen i1 2c3s 0 cidscasansesdus seven odeasesecsvacdesndesedenecoasteneys 114 Figure 89 Erase EEPROM Attention Message Screen oes eee ceeceeeeeeesseeseetseeesaeeenaeenes 114 Figure 90 About This Program Srila a rias 116 Figure 91 Transport A COESS ORY sia cso A 117 Figure 92 Cell A ak Sade es accede eee eens eis eee eee 118 Figure 93 Sipper ACCESSOLY sissies ceived ecesasdedeiadyiualacscsandeteandeh aasves r 119 Figure 94 Batch Sampler Accessroy visccaceracscecesanscaasiaccenassautecncefusedacedewecndes mwema 121 Figure 95 Peltier Temperature Controller iii ta 124 Figure 96 Diagram of Location and Size of the Beam in Sample Compartment 127 Figure 97 System Status Window Screen usada iii tias 130 Figure 98 Fuse Compartment li Aa 131 Figure 99 Location of Source Access Door on DU 800 cooooncconncccocccoccnconnconncnonccnnncnnncnnos 133 Figure 100 Unplugging UV Source Connector ooooccocccoccconnnononcnonanoncnnn nono nononcnnncnnncron amna 133 Figure 101 Unscrewing Thumb Screws securing UV Source cocococccnocccocncoonnnoncnancnonncnnno 134 Figure 102 Removine UV Source nahin deer A eae 134 Figure 103 Reset Source Hours Screen A A A A AA 135 Figure 104 Location of Source Access Door on DU 800 w wemeenaemmmnmenza 136 Figure 105 Unscrewing Thumbscrews Holding Visible Source w smmmwa 1
37. of Source Access Door on DU 800 6s Za 3 To unplug the connector for the UV source squeeze on the tabs on the sides of the connector then pull up to release Figure 100 Unplugging UV Source Connector m UR ges Connector WA D S Tab 133 DU 800 Spectrophotometer Installation amp Operating Instructions 4 Unscrew the two thumb screws that hold the UV source in position Figure 101 Unscrewing Thumb Screws securing UV Source 5 Remove the source by lifting it straight up Figure 102 Removing UV Source 6 Unpack the new source being careful not to touch the glass envelope on the source Carefully push the new source straight down until it is seated flat against the source housing Secure by tightening the two thumbscrews and then plug in the connector 134 DU 800 Spectrophotometer Installation amp Operating Instructions NOTICE Do not touch the glass envelope on the new source If it is touched clean with alcohol and a soft cloth 7 Close the source access door and tighten the thumbscrew Plug in the instrument power cord and turn the spectrophotometer on Then start the DU 800 System and Applications Software wait until the system has re initialized and press the Continue button to enter the software 9 Click on Reset Source Hours in the Diagnostic menu under User Diagnostics and reset the hours for the UV source Fig
38. on the system is in the lt Generic User gt mode The above frame shows the system in generic user mode Blank Status The blank status is shown in the Blank Status label and provides the time for the last blank Initially it reads BLANK REQUIRED and when the blank flag has been reset by the program Each time a parameter changes that can affect the reading the blank flag will be reset During the time it takes to read a blank the label reads Blanking and Last Blanked at lt time gt when a blank has been obtained The time format depends on the international settings of Windows UV Lamp Status When the UV lamp is off the UV lamp status label is empty During the warm up phase of the UV lamp the UV Lamp Status label reads Warming up UV lamp and the icon on the respective source button flashes slowly After the warm up the label reads UV Lamp On for lt time gt Status amp Control Elements Sources The Visible and the UV sources are represented by icon buttons They provide both feedback and control The sources can be turned on or off independently using the icon buttons or the items in the system menu The visible lamp is executed instantly The UV lamp requires a minimum warm up time of 30 seconds however a 30 minute warm up time is recommended 49 DU 800 Spectrophotometer Installation amp Operating Instructions Holders The currently selected cell holder is displayed in the Status amp Control
39. parameters that are applied to the lt Generic User gt are the system parameters 80 DU 800 Spectrophotometer Installation amp Operating Instructions Output Tab The Output tab includes the preferences for the screen and printer outputs The content is identical to System Options Output Tab Header Tab The Header tab includes the custom header information and the selection for the length of the application header This tab includes the Department and the Operator fields Although the content is different from the System Options tab of the same name which has the system wide Organization field both tabs serve the same purpose see System Options Header Tab Graph Tab The Graph tab includes the preferences for the graph window The content is identical to System Options Graph Tab Colors Tab The Colors tab includes the color preferences for the graph window The content is identical to System Options Colors Tab Administration The Administration item is only enabled when an administrator is logged on Other users have no access to the functions included in this sub menu The following administrator functions are accessible through the Administration menu System Options User Account Manager Delete Custom Applications System Audit Trail Source Scheduler Performance Validation Scheduler Backup Export System Restore Import System Add Software Option s Create Backup License File Security Enhancements
40. sample compartment cover Ready Help Calibrate Close Performing a wavelength calibration takes approximately 2 minutes The message Wavelength calibration in progress flashes during that time When it is finished the message reads Wavelength calibration finished 107 DU 800 Spectrophotometer Installation amp Operating Instructions Run Baseline Test The functionality of the Baseline Flatness Test takes a blank reading on air followed by a sample scan on air to test the flatness of the baseline from 200 to 900 nm Figure 81 Baseline Test Screen Baseline Test x Commands Function Help RMS Flatness 200 to 900 nm 0 000000 200 300 400 500 600 700 800 300 Wavelength in nm Help Scan Baseline Print Close Autoscale Y To test the baseline flatness follow these steps 1 Verify that both sources are turned on and that they have stabilized for at least one hour 2 Verify that nothing is in the sample compartment that can potentially block or disturb the light beam 3 Click on the Scan Baseline button or the respective menu item The instrument automatically performs the test displays the baseline and calculates the RMS Flatness The expected RMS Flatness should be less than 0 001A 4 You may click on the Print button or the respective menu item to print the baseline Set Scan Gains The scan gains are set at the factory prior to shipment The gains are checked and
41. see Blanking Method for more information In these cases the instrument will remind the user with the message BLANK REQUIRED Insert an empty cuvette or a cuvette with a blank solution in the cell holder at the measurement position and close the sample compartment 34 DU 800 Spectrophotometer Installation amp Operating Instructions Now click the BLANK button The Status amp Control Frame displays Blanking during the blanking and Last blanked at 11 13 AM when it is complete In this example two blank readings are taken one at 540 0 nm and one at 480 0 nm The system is now ready to provide accurate readings Reading Insert a cuvette containing a sample into the Single Cell Holder or the appropriate position s of the Multi Position Cell Holder and close the sample compartment To simulate a kinetic reaction we need a sample that changes absorbance over time For this example we will add a small amount of coffee creamer any brand will do to the top of a cuvette filled with water Do not shake the cuvette The dispersion of creamer will cause a change in absorbance over time Begin the run as soon as possible after adding the creamer Don t forget to close the door of the sample compartment before you start the run Also make sure to click the Dynamic Autoscaling item in the Axis menu to ensure that you will see the kinetic data in the graph window during the run 60 gt Read Now click on the
42. temperature controlled Peltier temperature controlled Single Cell Holder Rectangular Ambient Single Cell Holder Cylindrical Ambient Turbidity Cell Holder Single Ambient 6 Position Cell Holder Ambient Water temperature controlled Peltier temperature controlled 6 Position Microcell Holder Ambient 7 Position Cell Holder Ambient 8 Position Cell Holder Ambient 12 Position Microcell Holder Ambient Tm Microcell Holder Peltier temperature controlled These cell holders require a Peltier Temperature Controller and the High Performance Transport to remove the excess heat This cell holder requires the High Performance Peltier Temperature Controller and the High Performance Transport to remove the excess heat Refer also to the Installation Instructions Single Cell Holders P N 512874 and Installation Instructions Multicell Holders P N 512875 118 DU 800 Spectrophotometer Installation amp Operating Instructions Sipper Accessory The sipper consists of a peristaltic pump which is used to aspirate a sample into a flow cell After the sample is aspirated into the flow cell a reading is taken automatically Figure 93 Sipper Accessory A901287P Al The Sipper Installed item in the Sipper tab of the Accessories window must be checked when a sipper is installed The sipper parameters are in the same tab and may be optimized for the samples Refer also to the Installat
43. with Headers Tab Selected User Options for Ron Steiner lx Output Headers Graph Colors m Generic Header Department e Operator pg m Application Header C Short Essential Parameters Medium Common Parameters Long All Parameters OK Cancel Help The Organization and Department fields are text fields The information entered in the Organization field is applied system wide The department information serves as the default for the User Options and will be applied to the lt Generic User gt printout header Additional header information can be defined for each application see Methods Report Tab The Application Header allows the administrator or user to set the length of the application header for the printout There are three priority levels which are predefined 85 DU 800 Spectrophotometer Installation amp Operating Instructions Graph Tab The Graph tab includes the preferences for the graph window Figure 35 User Options Screen with Graph Tab Selected User Options for Ron Steiner X Dutput Headers Graph Colors M Grid Default IV Symbols JV Emphasize Selected Scan Dynamic Autoscaling Y Axis Default 3 D Offset Min Max C Small Abs 0 000 3 000 Medium wi wt o f 100 F The Grid option turns the grid on or off By default this option is checked The Symbols option turns the symbols for data poi
44. 0 and 300 0 nm But HALT before we take the first reading we want to change some parameters which is done in the Method window Editing a Method The lt Default Method gt for Fixed Wavelength provides a set of pre defined parameters For example there are 3 wavelengths which are specified as 200 0 250 0 and 300 0 nm IS Edit Method We want to change these parameter to fit our requirements Click on the Create Edit Method menu item or the Edit Method icon button This brings up the window shown below with the default parameters for the Fixed Wavelength application 19 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 14 Method Window for Fixed Wavelenght Before the Change lt Default Method gt Application Sampler Report Info m Read Mode m Calculation Mode Default Absorbance None Transmittance C Factor C Formula Number of Wavelengths 3 Wavelength Save As OK Cancel Help We want to change the number of wavelengths to 1 and the wavelength to 260 0 nm First select 1 from the Number of Wavelengths drop down list box You will see that the grid with the wavelengths definition is reduced to one 1 wavelength Now click on the cell in row 1 which shows 200 0 as the wavelength change the value to 260 and press Enter The wavelength is now set to 260 0 nm and your method window should look like the one shown below 20
45. 37 Figure 106 Removing Visible Source uri iia 137 Figure 107 Installing New Visible Source coooconnncnnocononnnonnconncnonoconnnona nono ncnnonnnnnnn ccoo nono 138 Figure 10s Using Peaking Tools sence ces A EE ie 139 Figure 109 Reset Source Hours CEM A ee ee a 140 Figure 110 DU 800 System Initialization Screen oe eee eeeeeeeeeeneeceeecneeeeeeeeaeeeaeens 141 DU 800 Spectrophotometer Installation amp Operating Instructions DU 800 Spectrophotometer Installation amp Operating Instructions Introduction OOOO O Overview The DU 800 Spectrophotometer is a PC controlled system intended for use in quantitative and qualitative analysis in biological and industrial procedures that require spectrophotometric measurements in the UV and visible region of the electromagnetic spectrum If the instrument is used in a manner other than as described the safety and performance of the instrument can be impaired The DU 800 Spectrophotometer operates in the wavelength range of 190 to 1100 nm and has a bandwidth of lt 1 8 nm The focused micro beam design provides a wide linear range and other specific benefits for small volumes and precious samples Various accessories are available to address micro volume samples and individual application requirements The control of the instrument data handling and data reduction capabilities are contained within the confines of the PC The frame oriented software provides a convenient and user fri
46. 408 Eastern Europe Middle East Africa Beckman Coulter Intl SA 22 rue Juste Oliver P O Box 303 1260 Nyon Switzerland Phone 41 22 994 0707 FAX 41 22 994 0700 South Africa Beckman Instruments Pty Ltd Johannesburg Postal P O Box 1949 Halway House 1949 Physical Stand 1A Fedsure Park Tonetti Street 1685 Halway House Johannesburg Phone 27 11 805 2014 or 27 11 805 1015 FAX 27 11 805 4120 150
47. AUTION The DU 800 Spectrophotometer PC and monitor must be plugged into grounded electrical outlets If the plug on the power cord is not compatible with the electrical outlet contact the local Beckman Coulter office Do not use the power cord in this case High leakage current Ensure proper grounding The following optional accessories also require a grounded electrical outlet Printer Batch Sampler and Peltier Temperature Controller The electrical requirements for the DU 800 Spectrophotometer Batch Sampler and Peltier Temperature Controller are listed in the following Table The electrical requirements for the PC monitor and printer can be found in the respective installation manual of each item 10 DU 800 Spectrophotometer Installation amp Operating Instructions Table 1 Electrical Requirements Frequency Hz Voltage VAC Current Amps Spectrophotometer 50 60 100 240V 10 3 0 a 100V 120V 10 220V 240V 15 automatically detects proper 50 60 10 08 voltage Ea Peltier Temperature 50 60 100V 120V 10 220V 240V 1 0 Controller 10 0 5 The number of boxes received will depend on the items ordered Minimally you will receive one box which contains the DU 800 Spectrophotometer and the shipping kit as well as regular accessories If purchased you may also receive a personal computer and the monitor in the box Additionally ordered accessories may be shipped in separate boxes Once
48. Access feature must be checked With the Password Renewal feature checked the user and the administration passwords needs to be renewed after a given time period By default this feature is disabled When the feature has been enabled and the renewal time exceeded the user must change his her password in order to complete the logon process The default renewal time is 90 days The administrator may change this parameter when the feature is enabled The entry is limited to 10 365 days 88 DU 800 Spectrophotometer Installation amp Operating Instructions The following message appears at logon if the password requires renewal Figure 58 Password Renewal Screen Password Renewal 3 Regulations reguire that you update your password at given time intervals Please continue to enter a new password in order to log on Do you want to continue Yes No When continuing the Password Renewal window lets the user update the current password No in the above message box or Cancel from the Password Renewal window will abort the logon With the System Audit Trail feature checked key events will be logged to an audit trail file By default this feature is disabled This feature records all major events as indicated by the check boxes The following actions will be recorded if checked User Logon including failed attempts and User Logoff Password change Start and end of data acquisition open run start to c
49. CDG Cedex Phone 33 0 1 49 90 90 00 FAX 33 0 1 49 90 90 10 E Mail bioresearch france beckman com Germany Beckman Coulter GmbH Siemensstrasse 1 D 85716 Unterschleissheim Lohhof Phone 49 89 35 870 0 Switchboard FAX 49 89 35 870 490 Gen l Fax Italy Beckman Coulter S p A Centro Direzionale Lombardo Palazzo F 1 Via Roma 108 20060 Cassina De Pecchi Milano Phone 34 02 953921 FAX 34 02 95392264 E Mail beckman_italia beckman com Netherlands Beckman Coulter Nederland B V Nijverheidsweg 21 3641 RP Mijdrecht P O Box 47 3640 AA Mijdrecht Phone 31 0 297 230630 FAX 31 0 297 288082 147 DU 800 Spectrophotometer Installation amp Operating Instructions Spain Beckman Coulter Espa a S A Avda del Llano Castellano 15 28034 Madrid Phone 34 91 383 6080 FAX 34 91 383 6096 E Mail bioresearch espana beckman com Sweden Beckman Coulter AB Archimedesvagen 7 Box 11156 S 16811 Bromma Phone 46 8 564 859 00 FAX 46 8 564 859 01 E Mail bc beckmancoulter se Switzerland Beckman Coulter Intl SA Swiss Domestic Operation 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Phone 41 22 994 0707 FAX 41 22 994 0700 Turkey Beckman Coulter Ltd Turkey E 5 Yanyol Fatih Cad 81410 Soganlik Kartal Istanbul Phone 90 216 309 1900 FAX 90 216 309 0090 United Kingdom Beckman Coulter U K Limited Oakley Court Kingsmead Business
50. Frame Buttons are used for the available cell positions and HOME The current cell position is indicated by a button in the pressed state With a transport the HOME button is shown HOME re aligns the transport and moves the transport to a fixed location Notice that HOME is not a measurement position The user may control the transport or cell positions using the cell buttons when Manual has been selected as the Number of Cells Used in the method Single cell holders are represented by a single position The Cell Holder elements are as follows Any Cell Holder on a Static Mount An Auto 6 Cell Holder on a Transport Single Cal a le afell lt Any Single Cell Holder on a Transport An Auto 12 Cell Holder on a Transport ME IME Hi 1 2 3 4 5 6 ls Single Cell Holder Sm 8 9 10 11 12 50 DU 800 Spectrophotometer Installation amp Operating Instructions Applications Frame The applications area holds the applications menu and all elements related to the current application such as the grid for data and results graphed wavelength scan data etc Figure 30 Applications Frame File Help Abs 200 0 nm 0 9820 Abs 250 0 nm y r Abs 300 0 nm 0 8914 0 0762 Abs Abs Abs Sample ID 200 0 nm 250 0 nm 300 0 nm Ll The appearance of the applications frame is dependent on the respective application The applications are described in the Applications Software section Permane
51. Frame or click on the Save File icon button The Save File dialog appears which lets you enter an appropriate filename Click on the OK button to save the data or Cancel to abort If you don t change the target directory the data file DUX is saved to the following location C DU800 Data Generic User Kinetics lt Filename gt dux if the administrator has not changed the default DATA root directory previously NE Clear Clear To end the current task and reset the application e g for another task or to leave the application select Clear from the File menu in the Applications Frame or click on the Clear icon button A message will inform you if the data has not been saved The data grid and the graph are cleared any unsaved data is discarded and the application is reset You may then start another Kinetics Time run or select another application 38 DU 800 Spectrophotometer Installation amp Operating Instructions System Software General The CD ROM includes the DU 800 System and Applications Software setup files the installation and operating instructions and all installation instructions for accessories as Adobe Acrobat PDF files This section focuses on System Software and describes the user interface and generic functionality The applications are described in Applications Software The software has been optimized for a screen resolution of 800 x 600 pixels or higher as well as the use of Small Fonts
52. Operating Instructions 7 Insert the peaking tool into the adjustment hole on the front corner of the source mounting bracket Figure 108 Using Peaking Tool 10 11 Peaking tool Turn the peaking tool a small amount in the clockwise direction and observe the readings The goal is to achieve the lowest reading possible If the readings are lower than 0 Abs continue turning clockwise until the lowest reading is obtained If the readings are above 0 Abs turn counter clockwise until the lowest reading is obtained If the readings reach 0 3 Abs restart the run with a new blank reading Repeat the above steps until the lowest reading is obtained Tighten the two thumbscrews that secure the visible source mounting bracket See Figure 2 Close the source access door and tighten the thumbscrew Click on Reset Source Hours in the Diagnostic menu under User Diagnostics and reset the hours for the visible source 139 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 109 Reset Source Hours Screen Reset Source Hours 140 DU 800 Spectrophotometer Installation amp Operating Instructions Troubleshooting SS Ul Startup Diagnostic Tests When the DU 800 System and Applications Software is launched and an instrument is detected on the USB port the software downloads the firmware to the DU 800 builds the wavelength calibration table and then executes a series of diagnost
53. RP Scan x Commands Function Help 300 500 700 900 1100 Wavelength in nm Help DRP Scan Print Close The DRP scan window is very similar to the Baseline or Run Baseline Test window Autoscale Y The Commands menu items perform the same functions as the buttons The Axis menu items are described in RediScan The default Wavelength parameters are 190 0 nm and 1100 nm range from 190 0 nm to 1100 0 mm The default Counts parameters are 0 to 50 000 range is from 0 to 50 000 The Blank scan is taken automatically before the actual DRP scan The scan can be printed but not saved to a file 113 DU 800 Spectrophotometer Installation amp Operating Instructions Blocked Beam Noise More information about this function is available in the DU 800 Service Manual Figure 88 Blocked Beam Noise Screen Instrument Serial Number 123456789012 Wavelength 220 0 nm Intitial Reading 0 023822 T 0 023134 0 023318 0 022158 0 022208 0 024099 0 021056 0 024043 0 024258 0 023836 0 021181 0 023822 Erase EEPROM More information about this function is available in the DU 800 Service Manual Figure 89 Erase EEPROM Attention Message Screen Erase EEPROM X x ATTENTION This function will erase all wavelength calibration and scan gain data Instrument reset will be required OK Cancel 114 DU 800 Spectrophotometer Installation amp Operating Instructions Help Menu C
54. Spectrophotometer Installation amp Operating Instructions Getting Started A General Figure Make sure that you have reviewed the section Introduction before you begin to explore the system and its operation In order to make measurements the DU 800 System and Applications Software must be running and the DU 800 Spectrophotometer must be initialized The section Launch and Initialization describes the necessary steps The following step by step instructions will lead you through the basic applications using simple examples Please refer to Applications Software for more information Install a cell holder in the sample compartment of the instrument Configure the software for this particular holder in the Transport Holder tab of the Accessories window For the following examples a Single Cell Holder should be installed and configured With a Multicell Holder installed and configured up to 12 samples can be automatically processed within a set However this is not described in the following step by step exercises In this case it is recommended to set the number of cells in the Sampler Tab of the Method of the selected application to 1 before starting a sample run and use a single cell position By default the system uses the lt Generic User gt level 12 User Display Screen DU 800 ere pe lt Generic User gt BLANK REQUIRED Visible UV Single Cell Holder The system includes a Visible Lamp Tungsten
55. With a lower resolution the main window will not fit on the screen and the selection of Large Fonts or Other will result in the misalignment of controls Default Directories By default the software will be installed in the Program Files path or the path specified during setup The default installation directory is DU800 System Subdirectories and files that are created and kept beneath this directory such as methods etc are considered system related and not data related Directory Level Description CA Program Files DU800 System INSTALL Root Audit Trail For Audit Trail files DU800 Audit Trail log System Backup Files For Backup Export files My System backup bkx Custom Applications Diluted DNA mth Methods Generic User Fixed Wavelength My Mehod 1 mth My Method 2 mth Wavelength Scan My Method 1 mth Holds custom applications Methods Directory Generic Level Administrator Methods Directory Administrator Level User Name 1 Methods Directory User Level User Name 2 Methods Directory User Level Executable Includes Program Files don t touch Help Includes HTML Help File s PDF Adobe Acrobat PDF Files 39 DU 800 Spectrophotometer Installation amp Operating Instructions Data files that include readings results and scans are located in the data root or DATA directory which is separate from the installation directory As an exception backup files for methods are located in the Backup Files
56. a Window Help JOSH Sar BAS O c B fm AL ZL le B 1007 D37 A B C Data Acquired Company Institute Department Operator Comment Product Name Lot Read Mode Calculation Mode Number of Wavelengths Wavelength 1 250 2 300 Formula Unit 250 0 nm 300 0 nm Sample ID Abs Abs The CSV file is recognized by compatible programs and will load the data automatically for further analysis To create a CSV file simply click on the Data My Sample ID 1 0 0374 0 0323 Sample 2 1 0 0374 0 0323 Beckman Coulter Inc Bioresearch Development Center Steiner This is a note Abuprofin 1234 Abs Formula 2 w350 wi400 0 x 1000 ng ml Results ng ml 12 4939 9 4832 Export function in the Jools menu and the curent data will be exported to a CSV file The Data Export menu item is only available when data has been acquired or when a file has been loaded Selecting this menu item will display a common File Save dialog The format of the CSV file follows these guidelines Text information is embedding in quotes to make sure intended commas are not interpreted as delimiters All Header fields are text All Labels are text 77 DU 800 Spectrophotometer Installation amp Operating Instructions Example File Data Acquired 22 May 2000 03 50 PM Company Institute Beckman Coulter Inc Department Bioresearch Development Center Operator Steiner
57. a grid and the graph are cleared any unsaved data is discarded and the application is reset You may then start another Fixed Wavelength task or select another application Taking a Wavelength Scan In comparison to all other applications the Wavelength Scan application I or II takes scans which are represented by a continuous wavelength range e g readings from 200 0 nm to 800 0 nm in 1 0 nm intervals Make sure that the Wavelength Scan application is selected and that the current method is the lt Default Method gt by checking the drop down list boxes in the Toolbar If not click on the down arrow of the drop down list box and select the appropriate item You should see a window similar to the one shown below 25 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 18 wa DU 800 UV Vis Spectrophotometer DNA Group USBO01 Window with Wavelength Scan Application Selected lol x System Applications Methods Tools Diagnostic Help Wavelength Scan F RediRead fil Cell Match gt OpenFile mst Accessories ET C scan B A STOP lt Default Method gt F RediScan Je Clear tel SaveFile Edit Method un Method Parameter Value File Mode Function Axis Help Scanning Sampling Device Holder Holder Type Single Cell Number of Cells 1 Scan Mode Abs Start Wavelength 200 0 nm End Wavelength 800 0 nm Wavelength Interval 1 0 nm Scan Speed 1200 nm
58. able which is level flat and capable of supporting its weight and the weight of all accessories The instrument is designed to operate in a clean laboratory environment free from dust fumes excessive moisture and corrosive chemicals It should not be exposed to drafts from heating or cooling vents heating elements open windows or doors Lab areas that receive direct sunlight should also be avoided An ambient temperature of 15 40 C 59 104 F should be maintained Relative humidity should be 85 or less Instrument performance can be affected by strong electromagnetic fields that can exist in the proximity of large electric motors centrifuges diathermy machines and microwave sources Spectrophotometer Figure 3 DU 800 Spectrophotometer The DU 800 includes e DU 800 UV Visible Spectrophotometer optical bench e System and Applications Software CD ROM e USB Cable 6 foot Oare 48753 a e PC with pre installed software if ordered DU 800 Spectrophotometer Installation amp Operating Instructions Computer PC A computer PC is required to operate the DU 800 UV Vis Spectrophotometer Minimum Requirements Standard PC 266MHz or faster Available USB Port 128MB RAM 3 5 inch Floppy Drive CD ROM Drive Microsoft Windows 2000 Beckman Coulter fully supports tested PC models which includes the optional PC that can be ordered from Beckman Coulter Other PC s that meet the minimum requ
59. ample temperature with the Peltier Temperature Controller the High Performance Transport is required to remove the excess heat from the cell holder The Peltier method of temperature control allows both heating and cooling of the sample hence the ability to cool below ambient temperature If use of the Peltier Temperature Controller is desired make sure the Temperature Controller Enabled box in the Temp Controller tab of the Accessories window is checked 123 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 95 Peltier Temperature Controller A901285P Al Please notice that the glass silica or guartz of the cuvettes provides insulation between the sample contained in the cell and the cell holder which is heating cooling the sample A moderate amount of time 3 minutes is required to heat the sample after the cell holder has equilibrated at the desired temperature The temperature controller is turned on using the switch on the back When turned on the POWER indicator light on the front of the controller is illuminated continuously When the temperature controller is activated the OPERATING indicator light on the front of the controller blinks and the cooling fan located under the transport turns on High Performance Transport only The fan remains on for about 5 minutes after the temperature controller is disabled to remove the remaining heat from the cell holder Peltier temperature contro
60. ample to stop flowing and to stabilize A settling time of at least 1 0 second is recommended Speed The speed of the pump is controlled with the speed parameter A faster pump speed will aspirate samples faster For typical aqueous samples a pump speed of 8 is recommended Viscous samples may require a slower pump speed because of the surface friction developed when moving through the tubing Organic samples with a low vapor pressure may require a slower speed to reduce evaporation Reduce the pump speed if splattering occurs when samples are returned See Accessories Sipper Accessory for additional information Batch Sampler The Batch Sampler is used to fully automate the measurement of a large number of samples The Raise Arm Advance Lower Arm Arm to Wash and the Arm to Sample buttons may be used to control the batch sampler manually Figure 35 Accessories Screen with Batch Sampler Tab Selected Accessories x Transport Holder Sipper Batch Sampler Temp Controller m Batch Sampler C None Raise Arm Advance Lower Arm ISCO Beckman Arm to Wash Arm to Sample r Pump Parameters Wash Between Samples Fill Time 3 0 Sec z Return Mode ime 3 0 Betun Time Waste Sample No Retum Read Delay Time 0 5 Sec Forward Flush 80 Return Wash Time 3 0 Sec Reverse Flush 200 Return C Lower Flush 200 Return Slow Speed Fast Cancel Help Batch Sam
61. an only be accessed through a specific password System Status This window displays common information about the spectrophotometer and the operating system When the lamp time approaches 100 of the typical life expectancy the administrator might consider replacing the respective lamp in order to avoid interruption when the lamp actually burns out The Print button outputs the information displayed in this window The Computer Info button calls a separate Microsoft program which provides detailed information about the computer 106 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 79 System Status Screen System Status X Administrator Paul Novak Lab 101 Model DU 800 Instrument Name 04305 Serial Number 800 00627 Firmware Version 1 26 Visible Source Usage 752 3 hours 38 of typical life expectancy UY Source Usage 1297 1 hours 130 of typical life expectancy Windows Version 2000 Build 5 09282 Software Version 1 00 Language Enalish Help Print Computer Info Close User Diagnostics Calibrate Wavelength This function is used to calibrate the wavelength drive if the wavelength accuracy is not within the expected range The wavelength accuracy can be tested with the Performance Validation program Figure 80 Wavelength Calibration Screen Wavelength Calibration X 1 Turn UY and Visible lamps ON 2 Remove sample from sample compartment 3 Close
62. ator can specify a read average time from 0 05 to 99 9 seconds in all applications except Wavelength Scan and RediScan Background and sample scans are collected without averaging Smoothing may be used to improve the appearance of scan data Smoothing A wavelength scan can be smoothed using a selectable smoothing function The calculation using the Savitzky and Golay coefficients as modified for end points by Peter A Gorry is done for every data point in the scan using the data points before and after the point of interest The software selects the degree of smoothing based on the user selection Medium smoothing uses a calculated amount of data points and causes a pre defined degree of smoothing Light smoothing uses half the calculated data points and therefore causes a lighter degree of smoothing Heavy smoothing uses twice the calculated data points and therefore causes a higher degree of smoothing 1 Savitzky A and Golay M Anal Chem 36 1964 p1627f 2 Gorry Peter A Anal Chem 62 1990 p570f DU 800 Spectrophotometer Installation amp Operating Instructions Installation This chapter describes how to install the DU Series 800 Spectrophotometer and the required personal computer Installation by a qualified Beckman Coulter Field Service Engineer can be purchased by contacting your local Beckman Coulter sales office The DU 800 UV Visible Spectrophotometer is designed to sit on a laboratory bench or t
63. ault Off Trace or Annotate Grid The default of this item is based on the system or user options setting The grid can be turned on or off This setting is temporary and will not be retained 73 DU 800 Spectrophotometer Installation amp Operating Instructions Axis Menu Set Scan Limits Autoscale x Autoscale Y Autoscale Both v Abs 1 Set Scan Limits This item brings up a window which allows the user to set both the x and the y axis parameters manually The Y Axis limits are based on the current read mode To modify the current wavelength range change the X Axis Limits Figure 46 Set Scan Limits Screen Set Scan Limits X Y Axis Limits Lower 0 0 Upper 3 0 Axis Limits Lower 200 0 Upper 800 0 Cancel When OK is clicked the graph window will be refreshed Autoscale The Autoscale X Autoscale Y and Autoscale Both items are enabled when data is available These items automatically and immediately scale the respective axes Dynamic Autoscaling This item is checked or unchecked depending on the system or user options setting Dynamic autoscaling means that the y axis will be re scaled during data acquisition The menu setting is temporary and will not be retained 74 DU 800 Spectrophotometer Installation amp Operating Instructions Read Display Mode The Abs and T items indicate the current read mode by a check mark When clicke
64. avelengthiqwertty dux March 27 2002 9 50 4M Imwong End of data acguisition March 27 2002 9 51 AM mwong Start of data acquisition March 27 2002 9 51 AM Imwrong Data acquisition aborted March 27 2002 9 51 AM myvong Data acquisition aborted March 27 2002 9 51 AM myvong End of data acquisition March 27 2002 9 51 AM mwong Logoff March 27 2002 9 51 AM Administrator Logon March 27 2002 9 52 AM Administrator Start of data acquisition March 27 2002 9 52 AM Administrator Save file C DU800 Data Administrator Kinetics Timetasdfghj dux March 27 2002 9 52 AM Administrator End of data acquisition March 27 2002 9 52 4M Administrator Load file C DUSDO Dataimwong Fixed VYavelengthiqwertty dux March 27 2002 9 52 AM Administrator Save method KT method March 27 2002 9 53 AM Administrator Password changed for user mwong March 27 2002 9 53 4M myvong Wrong password r March 37 INNI QER AM Backup mizani Print Close Larson tailed When the Backup button is selected the entire active audit trail is automatically saved in the Audit Trail directory located in the INSTALL directory A copy of this backup file is also created in the DATA directory With the System Audit Trail successfully backed up the software will begin recording a new audit trail The entries of the last two weeks will be retrieved to provide overlap and continuity 92 DU 800 Spectrophotometer Installation amp Opera
65. bd Level 3Subduectory 1 Add User Level OK cu Level 1 of the subdirectory tree is fixed and represents the application which is the application name The application can be the name of a fixed application of the name of a Custom Application Level 2 of the subdirectory tree can be any one of the following Product Name Component Name Lot Number These are method parameters from the Report tab that can be freely determined by the user or the administrator who may provide the methods for the controlled system When the selected entry is empty the name Unknown Method is applied giving a clear indication that a valid entry must be supplied 104 DU 800 Spectrophotometer Installation amp Operating Instructions With Use Custom Settings the parameter that has been selected as Level 2 will be disabled in Headers for Current Run so it cannot be overwritten by the user Figure 77 Headers for current run Headers for Current Run x M Generic Header Department Quality Contr Operator Steiner Ran Product Name Cyclobenzaprin Component Name Carbonate tsti Cs S Lot Number IS Comment uu Cancel Help Level 3 of the subdirectory tree is optional and is not used by default When the parameter iAdd User Leveli is checked an additional subdirectory with the name of the logged on user will be applied Enhancements The window with the
66. blanked at 11 13 AM when it is completed In this example a blank scan is taken from 500 0 nm to 800 0 nm with an interval of 2 0 nm at a scan speed of 2400 nm min The system is now ready to provide accurate scans Scanning Insert a sample into the Single Cell Holder or the appropriate position s of the Multi Position Cell Holder and close the sample compartment 28 DU 800 Spectrophotometer Installation amp Operating Instructions The grid below the scan window will be automatically populated when we take scans The Use check box allows you to select if the scan should be used and displayed or not By default each acquired scan will be used and displayed O Scan Now click on the SCAN button to take a scan You can see how the readings for each wavelength within the range are taken in real time If the Dynamic Autoscaling item in the Axis menu is checked the y axis will be automatically resized during the scanning process If not you can select the Autoscale Y item in the Axis menu to autoscale the y axis manually when the scan is finished You may now open the sample compartment replace the cell with the next sample or sample set when using a multi position cell holder and then close the sample compartment to read the next sample Your window should now look like the one shown below Figure 21 Window with Wavelength Scan Application and Scans Taken From Three Samples Using a Single Cell Holder
67. ckup file in a Save Backup File common dialog box The initial directory is System Backup Files in the INSTALL root directory and the initial filename is Untitled bkx The Backup Export System function backs up all segments a User Accounts and Methods b System and User Options c Custom Applications The installed applications are not included in the backup Restore Import System The Restore Import System function restores selected segments from a system backup file 96 DU 800 Spectrophotometer Installation amp Operating Instructions First the administrator selects a backup file in an Open Backup File dialog box The initial directory is System Backup Files in the INSTALL root directory Figure 67 Restore Import System Screen Restore Import System x m Segments to be restored Overwrite V User Accounts and Methods IT MV System Options IV Custom Applications m Cancel By default the Overwrite options are unchecked The following rules apply for restoring information 1 Users Accounts and Methods Non existing user accounts Users will be added with all passwords methods and user options retained Existing user accounts With the Overwrite option unchecked only non existing methods will be restored With the Overwrite option checked all existing passwords methods and user options will be replaced System Options The system options will be restored and overwritt
68. colors The items in the Graph Colors frame include the settings for the Window Background the Graph Background and the Grid The items in the Scan Colors frame determine the color of the individual scans The Default button resets the preferences of this tab 87 DU 800 Spectrophotometer Installation amp Operating Instructions Regulatory Tab The parameters in the Regulatory tab also apply to Title 21 Code of Federal Regulations 21 CFR Part 11 Electronic Records Electronic Signatures Figure 57 System Options Screen with Regulatory Tab Selected System Options X Setup Output Headers Graph Colors Regulatory m 21 CFR Part 11 Default Electronic Signature No lt Generic User gt Access Password Renewal Renewal after 90 Days m T System Audit Trail VV Logon Logoff V Change Password J Data Acquisition M Eile Load 5 Recommend Backup after 365 Days Recommend Backup when file exceeds 128 KBytes ave OK Cancel Help The Electronic Signature feature is unchecked by default When checked it allows authorized users to add and an electronic signature to a data or results file The signature itself is based on the logon as well as other information and is unique see section Electronic Signature for more information In order to restrict the use of the system to logged on users and prevent unauthorized access the No lt Generic User gt
69. cted mode and wavelength range Clear will clear the scan window Print will print the current scan s and Close will close the window Function Menu Function Default Off Trace Annotate Zoom v Grid Trace Trace is used to display the graphed data at the trace line position of the x axis The menu item is disabled when there is no data By default the Trace function is inactive When selected the cursor in the scan window becomes a two pointed horizontal arrow As the trace line is moved across the screen using the mouse the displayed values will be updated In the case of curve fits the actual data point values will be displayed when the trace line is near a data point Within the scan window the left mouse button alternately removes and activates the trace line In the active state the cursor is limited to the scan window The data is shown in the respective color Trace is de activated by selecting another function such as Default Off Annotate or Zoom Annotate The Annotate menu item is disabled when there is not data When the Annotate menu 1s activated or checked the cursor in the graph window becomes an A character 72 DU 800 Spectrophotometer Installation amp Operating Instructions This function applied to graphic areas By clicking on a location within the graph window a realizable box opens in which text can be entered The text will be fixed to the location when Ent
70. d the scan s will be re displayed immediately The menu setting is temporary and will not be retained Cell Matching A Cell Match When a multi position cell holder is used the Cell Matching function may be applied to correct for differences in the cells Cell matching is performed using two or more cuvettes filled with a blank solution that are placed in a multi position cell holder The cell matching will be performed at the wavelength s selected Up to three wavelengths can be used When the Match Cells button is pressed the first cuvette is blanked and the value set to zero Then each following cuvette is measured and assigned a value relative to the first cuvette These values are displayed in the grid as dAbs and d T Figure 47 Cell Matching Screen Cell Match Parameters Wavelength nm Number of Wavelengths E gt JE 480 0 2 520 0 Use Cell Match 3 600 0 480 0 nm 520 0 nm Match Cellls Print Close Help When the Use Cell Match box is checked all readings taken by the instrument in any application except Wavelength Scan will be corrected by the values displayed in the cell match window This correction can be disabled and enabled without losing the correction values The status bar indicates if the cell match correction is turned on or off 75 DU 800 Spectrophotometer Installation amp Operating Instructions NOTICE When using cell match make sure that the wa
71. d using either the Calibrate Wavelength or the Set Scan Gains function It may be desirable to repeat the above procedure with nothing in the sample compartment after changing the gain for a significantly absorbing blank 109 DU 800 Spectrophotometer Installation amp Operating Instructions Reset Source Hours This function resets the internal usage counter for both the Visible Source and the UV Source Click on Reset Hours for Visible Source or Reset Hours for UV Source to reset the counter Figure 83 Reset Source Hours Screen Reset Source Hours x ATTENTION Please DO NOT reset the source hours unless the respective source has been replaced Visible Source 752 3 hours UW Source 1297 1 hours Reset Hours for Visible Source Help Reset Hours for UY Source Close A message will prompt the user to confirm the action or abort NOTICE Do not reset the usage counter unless the respective source has been replaced Valuable service information will be lost otherwise 110 DU 800 Spectrophotometer Installation amp Operating Instructions Service Diagnostics The Service Diagnostic function is password protected and will only be accessed by service personnel and manufacturing Figure 84 Service Diagnostics Screens Service Diagnostics x Service Diagnostics X Enter service password Enter service password E Moo Enter new password Change Password rr Re enter to confi
72. directory of the DATA directory The default directory for data files is CADU800 Data The directory location may be changed by the administrator Directory Level CA DU800 Data Performance Validation PV Results 000818 txt Generic User Backup Files My Methods Backup bkx Fixed Wavelength My Data dux My Data 2 dux Wavelength Scan My Data 1 dux Diluted DNA My Data 1 dux Administrator Backup Files User 1 Backup Files File Extensions Description DATA Root Holds Performance Validation result files Data Directory Generic Level For Backup Export files Holds backup methods Applications specific directory Applications specific directory Custom Applications Directory Data Directory Administrator Level For Backup Export files Data Directory User Level For Backup Export files The following represent valid file extensions File Extension Description MTH Methods Database DUX Scans Data and Result files BKX Backup Export files TXT Performance Validation Result files LOG Audit Trail files Passwords The Administrator password provides access to the administrator functions The default administrator password is admin This initial password should be changed by the administrator after installation The administrator sets up and maintains user accounts with the User Account Manager and establishes initial user passwords From this time on forward the user updates his her own password without the involv
73. dministrator Screen sswswmmnmnmannmnimnmmanwa 53 Figure 33 Accessories Screen with Transport Holder Tab Selected v 54 Figure 34 Accessories Screen with Sipper Tab Selected wwwwwwmmwmmmawe 55 Figure 35 Accessories Screen with Batch Sampler Tab Selected s 56 Figure 36 Accessories Screen with Temp Controller Tab Selected v 59 Figure 37 Delete Current Method Screen sita ii 63 vii DU 800 Spectrophotometer Installation amp Operating Instructions Figure 38 Copy Methods from Other User Screen oooconnoconoccconanonancnoncnonnnnn cnn nono nonnnccnnnoo 63 Figure 39 Copy Methods to Custom Applications Screen occoocccnncnnnonononononoconnnonncnncnnos 64 Figure 40 Backup Export Methods Screen ooocccoccccnoncccnonccononcnononcnononononnnncnonccconnnccnnncninnnos 65 Figure 41 Restore Import Methods Screen ooooococcnnccnnonnconncnonononnnono nono nonnc nan ccoo anno ncnnnn ninas 66 Pigur 42 Redikead iwa 69 Figure 43 RediRead Dialog Screen ii ee ee 70 Figure 44 RediSean Screen A A eee 71 Figure 45 Scan Without a Blank Dialog swwsmmsnmnnmanmanmnnmwnmnmanwnmnman nia 72 Figure 46 Set Scan Limits Screen i 12 3h05 weed Gendt did 74 Figure 7 Cell Matching Sereen cajetilla deiig siii 75 Figure 48 Example of Data Exported as a CSV File oo cece eeseesseceseeeseeeeaeeneeetseeennees
74. e a customizable data directory structure and another common enhancement Only the administrator can activate these features which will affect how logged on users interact with the system Some of these features are designed to give a company tighter control in regard to system usage data acquisition and storage The new features of Build 74 are accessible through the Security Enhancement item in the Administration menu Figure 71 Administration Menu RediRead Redi5can Cell Matching Data Export View Electronic Signaturets s Add Electronic Signature User Options Administration System Options User Account Manager Delete Custom Applications System Audit Trail Source Scheduler Performance Yalidation Scheduler Backup Export System Restore Import System Add Software Option s Create Backup License File Security Enhancements Lock RediRead and RediScan This feature gives the administrator the choice to lock RediRead and RediScan The check box for this feature is located in the 21 CFR Part 11 Compliance tab of the Security Enhancements item in the Administration menu By default the parameter Lock RediRead and RediScan is unchecked When checked the menu items RediRead and RediScan in the Tools menu as well as the appropriate shortcut icons will be disabled for all users including the administrators
75. e Arm button Analyses with standards only Place the standard s in order starting with the tube directly under the aspirator tube Click on the GO icon The batch sampler will aspirate each standard read it return it as specified and move to the next position Standard readings are taken until either the last standard is read or the last position of the red stop rack is read Samples Place the samples in the tubes starting with the tube directly under the aspirator tube Do not leave any empty positions Click on the START icon The samples are analyzed The default sample identifications will be applied Sample n where n is a sequential number starting with 1 The analysis will terminate when the sample in the last position of the red stop rack has been read When the analysis is complete clean the flow cell using solvent then an alkaline solution and distilled water When the flow cell is clean leave it filled with distilled water It is important that the flow cell never be left to air dry At the end of the day the daily cleaning procedure described in the Section Cleaning of Flow Cells in the Installation Instructions Single Cell Holders P N 512874 and Installation Instructions Multicell Holders P N 512875 should be used Shortcuts in the cleaning procedure usually prove to be self defeating and 122 DU 800 Spectrophotometer Installation amp Operating Instructions should be avoided since a build up of c
76. e Warranty Registration after installation This will guarantee that your system will be registered and that the one year warranty is applied properly It also assures quick and easy access to the Beckman Coulter Product Support team for answers to your questions Customer Support 800 551 1150 Marketing Support 800 742 2345 For North America Only Warranty Registration The Warranty Registration form is included under separate cover If you have not done so please complete the form and return to the address indicated 145 DU 800 Spectrophotometer Installation amp Operating Instructions Beckman Coulter Offices North America United States Beckman Coulter Inc Headquarter 250 S Kraemer Boulevard Brea California 92821 USA Phone General 714 871 4848 Phone Sales 800 742 2345 Phone Service 800 551 1150 FAX 800 643 4366 FAX 714 773 8283 Canada Beckman Coulter Canada Inc 6755 Mississauga Road Suite 600 Mississauga Ontario Canada LSN 7Y2 Phone 800 387 6799 BCO FAX 905 819 1485 Latin America Mexico Beckman Coulter de Mexico Adolfo Prieto 1213 Colonia del Valle A P 44 100 Mexico D F 03100 Phone 52 5 559 1635 FAX 52 5 559 3941 E Mail jcamacho beckman com 146 DU 800 Spectrophotometer Installation amp Operating Instructions Europe France Beckman Coulter France S A Paris Nord II 33 rue des Vanesses BP 50359 VILLEPINTE 95942 ROISSY
77. e and install the appropriate drivers automatically When following the described installation procedure the required USB drivers will be installed automatically and the installation will complete In this case skip the following and go to Step 8 If the DU 800 System and Applications Software has not been installed yet or the drivers cannot be found for another reason a window similar to the one shown below will appear and the required drivers which are located on the DU 800 System and Applications Software CD ROM may be added manually However we highly recommend that you cancel the Found New Hardware Wizard window and install the DU 800 System and Applications Software before turning on the instrument If manual installation is necessary the DU 800 drivers can be found on the System and Applications Software CD ROM in the Window inf and the Window System 32 Drivers directories 15 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 11 Found New Hardware Wizard Screen Found New Hardware Wizard Welcome to the Found New Hardware Wizard This wizard helps you install a device driver for a hardware device To continue click Next lt Back Cancel NOTICE Refer to Troubleshooting Operational Failures if you encounter any problems during installation 8 You may now launch the software and operate the DU 800 Spectrophotometer see Launch and Initialization 16 DU 800
78. e minimum requirements see Requirements Computer Refer to the installation instructions included with the PC monitor and printer for information on how to setup and power on each item NOTICE The monitor can be placed on the top left side of the DU 800 Large monitors may restrict the movement of the sample compartment cover 14 DU 800 Spectrophotometer Installation amp Operating Instructions Software Installation 1 Power on the PC 2 Locate the DU 800 System and Applications Software CD provided in the shipping kit and place it in the CD ROM drive of the PC 3 The setup should start automatically after inserting the CD If it does not access the CD ROM drive from Windows Explorer and click on SETUP EXE to start the installation manually 4 Follow the on screen instructions to complete the software installation Power on the DU 800 The power switch is located on back of the DU 800 Spectrophotometer The green Power LED on the front of the DU 800 will illuminate NOTICE If the green Power LED does not illuminate turn off the instrument and check the fuse Directions for fuse replacement are provided in Maintenance Fuse Replacement See Troubleshooting Operational Failures for a complete list of problem resolutions 6 The PC will automatically detect the DU 800 Spectrophotometer connected via the USB port 7 When a DU 800 is plugged into the USB port Windows will try to identify the devic
79. ed when a sipper is installed Figure 34 Accessories Screen with Sipper Tab Selected Accessories xj Transport Holder Sipper Batch Sampler Temp Controller JV Sipper Installed m Sipper Parameters Fill Time 3 0 Sec Return Time 3 0 Sec Read Delay Time 0 5 Sec Slow Speed Fast Cancel Help Eill Time The fill time is the amount of time in seconds that the pump runs to aspirate a sample into the flow cell The fill time also determines the amount of sample that is aspirated The more viscous the sample the longer the pump will need to fill to aspirate a given volume The recommended minimum sample volume with a sipper is 1 0 mL If the sample will be returned after analysis to minimize contamination the sample solution should fill the inlet tubing the flow cell and the outlet tubing but not enter the pump tubing If the sample volume is critical the amount of time required to aspirate that amount of sample should be checked periodically Return Time The return time is the amount of time in seconds that the pump returns the sample when RETURN is pressed It is normally set to equal or slightly greater than the fill time 55 DU 800 Spectrophotometer Installation amp Operating Instructions Read Delay Time The read delay time is the amount of time in seconds that the sample is in the flow cell after the pump stops and before the reading is taken This allows the s
80. ement of the administrator See User Account Manager and Change Password for more information 40 DU 800 Spectrophotometer Installation amp Operating Instructions The Service Diagnostic programs are password protected and are only available to Beckman Coulter service personnel Logon Levels The DU 800 includes three levels of operation In all three levels all available applications are fully accessible using the default settings Generic or lt Generic User gt This is the default level and provides no methods data protection and no user customization User A user may log on with a user name and password The initial password is supplied by the administrator Only methods belonging to the individual user are shown and data are accessed and stored in user specific directories Each user can set and use his her own preferences in User Options A group of users such as personnel of a specific lab could also be considered as a single user Simply choose an appropriate name and share the password with the group Administrator The administrator may log on as Administrator and the administrator password Only methods belonging to the administrator are shown and data are accessed and stored using the administrator subdirectory The administrator can set the system preferences in System Options The system options apply to the lt Generic User gt and serve as the default preferences for the User Options See User Logon
81. en Custom Applications With the Overwrite option unchecked only non existing custom applications will be restored With the Overwrite option checked all existing custom applications will be replaced 97 DU 800 Spectrophotometer Installation amp Operating Instructions Add Software Option s The Add Software Option s function allows the administrator to install one or more software options Figure 68 Add Software Options s Screen Add Software Options s x G Do you want to install from a Floppy Disk 1 Jana The license is initially provided on a floppy disk and is usually installed from there Figure 69 Select License File Screen Look in 29 3 Floppy 4 va E ef EJ Enzyme Mechanism lic File name Enzyme Mechanism lic Files of type License Files ic y Cancel YA A dialog box will show the available license files on the selected medium No other file types will be shown The application s that are included in the selected license file will be displayed 98 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 70 Add Software Options s Screen Add Software Options s x lt gt Thank you For purchasing the Following software options 517004 Enzyme Mechanism Add the above software options now Pe Click on Yes to install the software option s Installed software options cannot be removed If the system encounters a file
82. en when the BLANK icon button is clicked This icon button is located in the toolbar NOTICE In the RediRead and the RediScan modes the blank is executed in the Commands menu or by clicking on the Blank button DU 800 Spectrophotometer Installation amp Operating Instructions When the instrument blanks the following steps are performed 1 The monochromator is moved to the proper wavelength This is the specified wavelength for a single wavelength reading 2 The proper detector gain value is selected automatically This minimizes the noise level and maximizes photometric accuracy 3 Dark current is measured and corrected This compensation assures accurate readings at high absorbance 4 In the Wavelength Scan application only a background scan is taken The blank or reference is automatically scanned over the same range at the same speed that the sample will be scanned so that the background correction is optimal This calibration assures repeatable readings every time the instrument is used In all modes a blank solution should be in the sample compartment during the blank It is suggested that the solvent used to prepare the samples be used for the blank However air no sample may be used A new blank reading should be taken each time the solvent is changed NOTICE Plastic cuvettes glass Pyrex cuvettes and some solvents have significant absorption in the UV region Verify that they transmit UV light
83. endly interface and therefore ensures a quick learning curve The PC must run Windows 2000 as an operating system The general operation of the software is described in System Software and the standard and optional applications in Applications Software Figure 1 DU 800 Spectrophotometer with Computer and Monitor DU 800 Spectrophotometer Installation amp Operating Instructions Standard Applications Software The following standard applications are available after software installation Fixed Wavelength Performs Absorbance or Transmittance readings for up to 12 wavelengths simultaneously A factor or custom formula may be applied to calculate final results Wavelength Scan Performs wavelength scans in Absorbance or Transmittance Acquired scan data are stored and may be used for various manipulations and calculations Includes the calculation mode to add subtract multiply and divide spectra Kinetics Time Simultaneously measures and analyses up to 12 rate reactions Data can be reviewed in real time and automatically calculated and printed The rate of the kinetic reaction is reported using a linear regression Nucleic Acid Analysis Determines protein impurity in nucleic acid samples based upon the ratio of readings at two wavelengths with a choice of background correction Protein and nucleic acid concentrations can also be calculated using the Warburg and Christian coefficients Single Component Analysis Det
84. entical functions only the terminology is different Change Password Each user is able to change his her password at any time Title 21 Code of Federal Regulations 21 CFR Part 11 Electronic Records Electronic Signatures reguires the periodic change of the user password See System Options Regulatory Tab for the necessary setup 52 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 32 Change Password for Administrator Screen Change password for Administrator X Enter old password Enter new password Confirm new password ik Cancel The OK button is disabled until the number of characters in the Confirm New Password field is identical to the Enter New Password field and the new password is confirmed in the Confirm new Password field A user account must be established by the administrator before a user can log on The User Account Manager which allows the creation of user accounts is strictly an administrator function Log Off The Log Off item returns the system to the lt Generic User gt mode and the Fixed Wavelength application Sources The menu items UV Source and Visible Source allow the operator to turn the lamps on and off The respective icon buttons in the Status amp Control Frame perform the identical functions Check marks are used to indicate the status of the lamps Accessories The Accessories item allows access to the setup window for the system acces
85. ention path may be entered or selected When defining a new DATA root directory complete path the directory will be created automatically if it does not already exist Both a local directory and an UNC 82 DU 800 Spectrophotometer Installation amp Operating Instructions Universal Naming Convention path will be accepted The Default button resets the parameters of this tab NOTICE When defining a DATA root on a network drive make sure that the required access privileges are available Instead of entering the path for the DATA root directory you may browse for an existing directory by clicking on the Browse button to the right of the text field By default the Idle Timeout Functions are unchecked The Automatic Logoff is required for 21 CFR Part 11 and will automatically log off the current user and return to the lt Generic User gt level when the system is idle for more than the specified time When a user is logged on and the system is in an open run e g it is busy with a Kinetics run instead of logging off the system continues the Kinetics run and displays a protected DU 800 screen saver The DU 800 screen saver is automatically selected whenever the DU 800 software is running and Automatic Logoff is selected When the software is exited the system will return to the previously selected screen saver The DU 800 screen saver will lock the computer until the current user or the administrator enters his
86. er Lamp Selector P functions The wavelength may not be accurate Use the instrument only if Wavelength Drive the accuracy of the readings can be verified Contact the Beckman Coulter service 142 DU 800 Spectrophotometer Installation amp Operating Instructions Operational Messages The following messages can occur while operating the instrument and are displayed in an error window In most cases these errors do not indicate an instrument malfunction but a situation where an action was initiated that the instrument cannot perform If the instrument requires operator action to continue the message is given in the form of a message window and the user must click on OK to remove the window Message Explanation and Recommended Action Blank Required Place a cuvette with solvent in the cell holder and click the q BLANK icon button Any reading taken without a blank is invalid Turn on the UV source Wait for the UV source to warm up and UV Lamp Required blank the instrument Any reading taken without the proper source on is invalid Visible Lamp Required Turn on the visible source and blank the instrument Any reading taken without the proper source on is invalid 143 DU 800 Spectrophotometer Installation amp Operating Instructions 144 DU 800 Spectrophotometer Installation amp Operating Instructions Service and Support Please make sure to complete and return th
87. er to create a new method based on the loaded file parameters Parameters stored within the file will be displayed in the Method Parameters list box However the Method Parameter column will be renamed to File Parameter Measurement controls such as READ or SCAN are used to take readings or scans These controls are disabled when a file has been loaded In the Method window parameters such as Analytical Wavelength etc are locked when a file has been opened because they are related to measured data Parameters that remain open can be modified to perform a post run analysis The data may then be saved under a different filename NO MEASUREMENTS CAN BE TAKEN WHEN A FILE HAS BEEN LOADED When opening a file or saving data the initial directory is the applications specific directory under the DATA root Each logged on user has his her individual directories These data directories are created automatically within each user directory and will be accessed by default for opening and saving data files The default directory for data is the user s directory in the DATA root If the DATA root is an UNC path and the system cannot connect to the network the user will receive the message Cannot currently connect to the network Do you want to use the local drive temporarily Yes No If yes the default root or program directory will be used NOTICE Existing DUX files cannot be overwritten Trying to save a file under the same name
88. er Temp Controller Temperature Controller Enabled Set Temperature to 250 C Cancel Help Manual Control The temperature controller is enabled by entering the desired temperature and checking the Temperature Controller Enabled box The input range is from 10 C to 90 C The range that is actually suitable for a 6 Position Cell Holder is from 20 C to 40 C Clicking the OK button will activate the temperature controller If the temperature controller does not activate the following warning message will be displayed The Peltier Temperature Controller could not be activated Please verify that the Temperature Controller High Performance Transport and the Cell Holder are connected properly and that the controller is powered on Automatic Control With Kinetics Time Enzyme Mechanism and Experimental Tm Analysis applications the temperature is controlled directly from the respective application Temperature control will be initiated within these applications if the Temperature Controller Enabled box is checked and if all the requirements for Peltier temperature control are 59 DU 800 Spectrophotometer Installation amp Operating Instructions met The temperature range that is actually controlled depends on the cell holder The Tm Microcell Holder has a much wider temperature range than other cell holders and significantly different control dynamics Applications Menu Standard and Cus
89. er is pressed By clicking on a location within the graph window a realizable box opens in which text can be entered The text will be fixed to the location when Escape is pressed The annotations and its locations are retained Within the annotation box a right mouse click brings up a popup menu which allows the user to fix delete or change the appearance of the annotation Fix Annotation Delete Annotation Font and Color An existing annotation can be moved by holding down the Ctrl key and clicking on or near the text with the left mouse button Releasing the mouse button will fix the annotation again Clicking on an existing annotation opens the annotation box and the annotation can be modified The annotations and its locations are retained and saved with the scan The number of annotations is not limited Annotate is de activated by selecting another function such as Default Off Trace or Zoom Zoom The feature can be used to expand any portion of the graph The menu item is disabled when there is no data When selected the cursor in the scan window becomes a magnifying glass To zoom in click the left mouse button and drag the mouse to another location When releasing the mouse button the system zooms in onto the rectangle that has been drawn The zoom can be repeated to zoom in further A double click on the right mouse button will zoom out Zoom is de activated by selecting another function such as Def
90. erating Instructions Figure 44 RediScan Screen RediScan X Commands Function Axis Help BLANKED Scan Speed 1200 nm min at 1 nm Resolution 200 300 400 500 600 700 800 Wavelength in nm Help Blank Clear Scan Autoscale Y Print Close The scan speed is fixed at 1200 nm min which represents readings at a 1 0 nm interval The default scan mode is Abs and the default wavelength range is from 200 0 nm to 800 0 nm Use the Set Scan Limits item in the Axis menu to modify the wavelength range over which the sample is scanned The system options set by the administrator act as default parameters for the y axis the grid and the legends Before taking a scan RediScan needs to be blanked on air or a blank solution This is done by clicking on the Blank menu item or button When a blank has been performed the indicator BLANK REQUIRED will change to BLANKED and the user is ready to take a scan NOTICE Because this mode is an_ independent program module the blank is owned by this mode and does not affect the blank of the application Trying to take a scan without a blank causes the following message to pop up Yes will scan the sample without a blank 71 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 45 Scan Without a Blank Dialog WARNING AN No blank measured Proceed anyway Yes No The Scan menu item or button takes a wavelength scan based on the sele
91. ermines the concentration of unknowns by either linear or non linear quadratic regression and provides statistical analysis for the standard curve The standard curve can have up to 30 standards The operator can re run any standard remove or add standards and have the instrument re calculate the curve Performance Validation Provides a simple procedure to verify the performance of the instrument without standards or samples Tests include wavelength accuracy and repeatability resolution baseline flatness noise and stability In addition the rapid modes RediRead and RediScan are available These provide the user with a fast and easy way to take readings at fixed wavelengths or to make wavelength scans A detailed description of the above applications can be found in Applications Software Other features such as User Logon User Customization Methods Backup and Transfer Diagnostics Source Scheduler and Performance Validation Scheduler are described in System Software Optional Applications Software Wavelength Scan II Extends the functionality of Wavelength Scan with the following modes Derivatives 1st 2nd 3rd and 4th NetA Calculations Scatter Correction Point Picker and Peak Valley Picker Also included is the display of spectra in LogA Nucleic Acid Analysis II Extends the functionality of Nucleic Acid Analysis with multiple ratios and other concentration calculations Also included are DNA RNA Oligo Quanti
92. essories to support micro volume sampling include microcell holders for up to 12 samples the 50 uL Microcell the 100 uL Multi Microcell and the 5 uL Ultra Microcell A detailed description of the above applications can be found in Applications Software 1 Warburg O and Christian W Biochem Z p 384f 1942 DU 800 Spectrophotometer Installation amp Operating Instructions Principles of Operation Optical Principle The DU 800 Spectrophotometer is a single beam instrument Light from both sources enters the monochromator where it is dispersed by a concave holographic grating Monochromatic light exits the monochromator and illuminates the sample The amount of light that passes through the sample is measured by a single photodiode detector Figure 2 Optical Diagram Schematic contains only major elements Deuterium SINA Source 1 Beam Combiner gt Tungsten A Source Transfer of Mirror Poco Sample l i ompartmen Mirror Transfer p Entrance ai a Mirror Slit Exit Slit Filter Sample yS Monochromatic Detector Ligh ght Depa Sample Grating Mirror The focal point of the beam in the sample compartment is on the right hand side All sampling accessories position the sample at the focal point for best performance with regular samples and micro samples Blanking A blank is always required before data collection any reading without a blank is invalid A blank reading is tak
93. ge v 21 Figure 16 Window with Fixed Wavelength Application and all Changes Made 22 Figure 17 Window with Fized Wavelength Application and Measurement Taken From Three Samples Using a Single Cell Holder ii 24 Figure 18 Window with Wavelength Scan Application Selected s 26 Figure 19 Method Window for Wavelength Scan After the Change v e 27 Figure 20 Method Parameters Frame After the Change o cece eeseesseceseeeeeeeeneeeeeneees 28 Figure 21 Window with Wavelength Scan Application and Scans Taken From Three Samples Usine aSinsle Cell AA dios 29 Figure 22 Window with Kinetics Time Application Selected e 31 Figure 23 Method Window for Kinetics Time Before the Change s smswe 32 Figure 24 Method Window for Kinetics After the Change oooooconoccnocccooncnoncnannnnnncnononanno 33 Figure 25 Window with Kinetics Time Application and All Changes Made 34 Figure 26 Data Acquisition Modest da cota ds San 36 Pioure 2 TRES Mode A A A Ad eee 37 Figure 28 DU 800 System Initialization Screen ooooconncnnnnnnnonononnnannnonnnnononnnnn nan nr nnncnnncnnnnos 42 Figure 29 Integrated Frame Oriented User Interface ooooconnnccnoncninccnocnconncnoncnancnnnncnononanno 44 Figure 30 Applications Frame 2 ee aed Nee ee 51 Figure 3 LDser Logon Screen a da 52 Figure 32 Change Password for A
94. her password Figure 52 User Authentication Screen User Authentication The Auto Logoff feature is active and the system has been locked after the specified time a to prevent unauthorized use Please confirm your password to continue User Profile Usemame Ron Steiner Password Cancel OK The Elapsed Time list box contains pre defined entries 1 2 5 10 20 30 minutes and 1 or 2 hours The default is 10 0 minutes 83 DU 800 Spectrophotometer Installation amp Operating Instructions If checked the UV Lamp Shutoff and the Visible Lamp Shutoff items turn the respective lamps off when the system is idle for more that the specified time idle means that no measurement has bee taken for a while The respective lamp will not be turned off when the systm is in an open run or a file has been opened Also the Source Scheduler has priority over the UV Lamp Shutoff and the Visible Lamp Shutoff functions Specifiy the Elapsed Time that should apply to the Automatic Logoff and the Lamp Shutoff functions Output Tab The Output tab includes the preferences for the screen and printer outputs Figure 53 User Options Screen with Output Tab Selected Output Headers Graph Colors m Screen Font Characteristics Default sells MS Sans Serif Change Font Size 8 pts Printer Font Characteristics Name Courier New Change Fark Size 10 pts of Decimal Points Highlight Results
95. ic tests to ensure proper operation of major system components As the tests are performed the entire progress and the individual results are displayed When the tests are complete the software may be used Figure 110 DU 800 System Initialization Screen x A COULTER System Software Download Complete Building WL Calibration Table Complete r Startup Diagnostic Tests Gain Passed Visible Lamp Passed Light Path Passed Shutter Passed Filter Passed Wavelength Drive In Progress Detector EEPROM MONCADA Abort Continue Print Following the test name is Passed or gt gt gt FAILED lt lt lt If any of the tests fail click on the Cancel button and then power down the instrument Wait a few seconds and then power it up again If the failure persists the instrument may be partially operational as described in the following table The Visible Lamp and Light Path failures can be corrected by the user If the problem persists contact the local Beckman Coulter service office If any other test fails contact the local Beckman Coulter office 141 DU 800 Spectrophotometer Installation amp Operating Instructions Failed Test Explanation Detector Readings cannot be takern The instrument can be used for other functions Gain Readings cannot be taken The instrument can be used for other functions Visible Lamp Replace the visible source Direc
96. ication The method name appears above the tabs in bold 1 When the Save As button is clicked the user can enter a new name or modify an existing name Clicking on OK or pressing Enter 2 saves the method under the given name The new method name 3 will be available immediately in the Methods menu bar and the drop down list box The first left tab is open by default and contains the key parameters for the selected application Tabs with additional parameters may be present depending on the application The Sampler Headers and Info tabs are common to all applications Additional information can be found in the Help and in the Software Reference Manual 62 DU 800 Spectrophotometer Installation amp Operating Instructions Delete Current Method This menu item is disabled with the lt Default Method gt selected Before a method is actually deleted the following message prompts the user to confirm the action Figure 37 Delete Current Method Screen El MY METHOD You are going to delete this method permanently Are you sure you want to continue JJ e Copy Methods from Other Users The list box shows all methods from other users One or more methods can be selected and copied to the current user by clicking on the Copy From User button The Copy From User button is enabled when one or more items are selected Figure 38 Copy Methods from Other User Screen Copy Methods from Other User xj Method Name Usemame
97. iii 40 AA A A Ae IN AN Shae OARS 40 LAS dos de e o e e 41 On Line Launch with System Initialization oooooccnoncnonccnnannnoncnoncnnncnnn ccoo cnn nonnnos 41 SA AAA O E E so AA MUA 43 Common Front Elda A is 43 User atera ii 43 M n Men A ian kawawa 44 Toolbarand Icons threes decades coset waw 45 BLANK GO READ SCAN and STOP Buttons w mewesmmewa 45 R diRead and RediScan iii 46 CoMo a 46 E ES 46 Open File Save File and Clear c cssesasssscsenacedctsasssnaaivanscdecnsdos canvassed anna 46 IROCESSORIES NS 48 Method Parameters Prime nad rios 48 Status amp Control Pra Ed 49 SUGGS AO AA A 49 User tallos nus lalala 49 Blank Statutes airada ladilla 49 UN LS AA IA AA 49 Status Control Elements iran 49 SOM a lid 49 HO 50 Applications Frame ii adeaehoduato anes aaen a aats 51 Permanentotatus Bai 51 Operation and Status Message si sssccerscdisssesensscoaredieessecesrasseamnavenacdesdasevensscoaaees 51 Spectrophotometer Statis WA 51 System MAA 52 User Logon Change Uria 52 Change PASS WOE AA ANA 52 DAG A AA AAA A E 53 NYA LI Ka ld asa 53 PROCESS La LA o TAN KAA WAKIRI 53 BA AA AI nd oh a a et ad 54 DU 800 Spectrophotometer Installation amp Operating Instructions Transport Hold a ode TE 54 o A O 55 Batch ampli A A 56 Peltier Temperature Comtrouer AK 59 Mana Ce GODS A AA 59 Automatic Control iii Ad 59 Applications Men is 60 Standard and Optional Applications coocccnonccc
98. iles PDF item calls the Adobe Acrobat Reader and automatically loads the respective Installation and Operating Instructions or other documentation The respective PDF files will be copied to the hard disk during the installation process to make the information readily available Adobe Acrobat might have been installed during the DU 800 System and Applications Software installation However the installation of Adobe Acrobat during installation is 115 DU 800 Spectrophotometer Installation amp Operating Instructions optional If a valid Adobe Acrobat installation cannot be found an error message appears In this case you might want to install or re install Adobe Acrobat Figure 90 About This Program Screen About this Program x P COULTER Product ID 512910 DU800 System and Applications Software for DU 800 UY Visible Spectrophotometer p Version 1 0 Build 001 Ron Steiner Beckman Coulter Inc Copyright 2001 Beckman Coulter Inc All rights reserved This software program is partially based on the Spectacle kernel from Creon LabControl WARNING This computer program is protected by copyright law and international treaties Unauthorized reproduction and distribution of this program or any portion of it may result in severe civil and criminal penalties and violators will be prosecuted to the maximum extent possible under the law Close The About this Program item provides program
99. ill be rebuilt The applications drop down list box is a shortcut and performs the same function All available standard and optional applications are described in Applications Software 60 DU 800 Spectrophotometer Installation amp Operating Instructions Custom Applications A custom application is a method from one of the existing applications with a link to the respective application A custom application may be used by any user to safely run a canned application The method parameters of a custom application cannot be modified By default this menu item is disabled It is enabled with one or more custom applications Regular methods can be copied to the Custom Applications and become a new application A method based custom application is associated with an existing application and protected from common method editing The limit for the number of custom applications is 30 Custom applications are publicly available applications and can only be deleted by the administrator Methods Menu Methods v lt Default Method gt My Method Ron Steiner Create Edit Method Delete Gurrent Method Copy from Other User Copy to Custom Applications Backup Export Methods Restore Import Methods lt Default Method gt Indicates if the default method is currently selected or allows the user to select it The method drop down list box in the toolbar provides a shortcut for this fu
100. ion Instructions Sipper Accessory P N 512873 Sipper Controls The sipper panel has four control buttons FILL BLANK Aspirates the sample for the set Fill Time waits the set Read Delay Time and then reads the sample as a blank RETURN Returns the sample for the set Return Time FLUSH Runs the pump in the forward direction as long as the button is pressed Used to flush the flow cell with solvent sample etc without taking a subsequent measurement After flushing the flow cell with solution the user may take a reading as an alternative to FILL READ FILL READ Aspirates the sample for the set Fill Time waits the set Read Delay Time and then reads the sample as a sample RETURN FILL BLANK and FLUSH are active at all times FILL READ is only active when Sipper and or Batch Sampler are selected in the Sampler tab of the Method window 119 DU 800 Spectrophotometer Installation amp Operating Instructions Sipper Operation 1 Verify that the desired fill time return time read delay time and sipper speed have been set Clean the flow cell by flushing with distilled water then an alkaline solution distilled water again and finally the solvent used for the analysis Several seconds with each solution should be sufficient for cleaning if the flow cell was cleaned after the previous use Make sure that Sipper is selected in the Sampler tab of the Method window Blank Aspirate the bla
101. irements and have the appropriate operating system installed should be compatible However this cannot be guaranteed by Beckman Coulter The optional PC from Beckman Coulter ensures a ready to use system and comes in a fixed configuration which is subject to change IBM PC with USB Interface 15 inch Monitor or Flat Panel Display Microsoft Windows 2000 or later pre installed DU 800 System and Applications Software pre installed The pre installed software is included as an image on CD ROM s which allows the user to restore the entire content of the hard disk Software Figure4 DU 800 System and Applications Software aa The DU 800 System and Applications Software has been Pan system a validated with the following local language Windows 2000 Operating Systems e U S English e German e Japanese The software was written following Microsoft Windows development guidelines and therefore should be fully compatible with other Latin and Asian languages DU 800 Spectrophotometer Installation amp Operating Instructions Follow the instructions found in paragraphs Unpacking Hardware Installation and Software Installation to install the system Space Requirements The PC must be located close enough to the spectrophotometer so that the interconnecting USB cable will reach the USB port on the instrument If desired the monitor can be placed on the top left side of the DU 800 spectrophotometer Large monitor
102. is mode is an_ independent program module the blank is owned by this mode and does not affect the blank of the application Trying to read a sample without a blank causes the following message to pop up Yes will read the sample without a blank 69 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 43 RediRead Dialog Screen WARNING AN No blank measured Proceed anyway Yes No The current wavelength and result are displayed in large characters in addition to the grid This makes it easy to walk up with a sample take a measurement read the result from the screen and walk away The Read menu item or button takes a reading based on the selected read mode and wavelength Clear will clear the grid Print will print the content of the grid and Close will close the window RediScan Fh Redi Scan RediScan provides an easy and fast way to take one or multiple wavelength scans and provide a printed output In this mode the system will not store data or perform calculations RediScan acts like an interrupt program that keeps the workflow of the current application intact Up to six wavelength scans can be taken displayed in an overlay fashion The cell position of a multicell holder cannot be changed from within the program To avoid overlaying scans simply click on the Clear menu item or button before taking the next scan 70 DU 800 Spectrophotometer Installation amp Op
103. ixed Wavelength Application and all Changes Made ka DU 800 U is Spectrophotometer DNA Group USBO01 0 x System Applications Methods Tools Diagnostic Help Fixed Wavelength gt gt MA RediRead fl Cell Match gt Open File a Accessories OCEN O 7 lt Default Method gt es Fh RediScan Je Clear LA Save File Edit Method Method Parameter Value File Help Sampling Device Holder Holder Type Single Cell Number of Cells 1 Read Mode Abs Abs 200 0 nm Calculation Mode None of Wavelengths 1 Wavelength 1 260 0 nm gt Read Average Time 0 5 sec Abs A 260 0 nm DU 800 A sexctreo tiie lt Generic User gt BLANK REQUIRED UY Lamp On for 1 min 19 sec a Visible UV Single Cell Holder i Ready CellMatchOFF 600 0nm 0 308Abs 248C 10Now2000 11 20AM y The following three things have changed when we closed the Method window First the Method Parameter List has been updated Notice that it only shows one wavelength with the entered wavelength value Second the data grid changed to only one column with the correct heading to reflect the current parameter settings And third the large number display has only the field that is labeled with the respective unit and wavelength We are now ready to measure our samples using customized parameters But before we take a reading of the first sample we need to blank the system on the selected wavelength B
104. l Source Scheduler Performance Validation Scheduler Backup Export System Restore Import System Add Software Option s Create Backup License File Both RediRead and RediScan provide an easy and fast way to take simple readings or scans These modes act like interrupt programs that keep the workflow of the current application intact When a multi position cell holder is used the Cell Matching function can be used to correct for differences in the cell positions 67 DU 800 Spectrophotometer Installation amp Operating Instructions To further process acquired data or obtain an independent readable file you may want to make it compatible with a commercial program using the Data Export function The currently displayed data may be saved as a CSV comma separated values file that is compatible with commercial spreadsheet programs such as Microsoft Excel Files may contain one or more Electronic Signature s The user may view the electronic signature s in a file that has been opened or add a signature to an existing DUX file if he she has the necessary privileges The User Options allow a logged on user to set his her preferences The Administration item is only enabled when the administrator is logged on The administrator has access to the following menu items System Options User Account Manager Delete Custom Applications System Audit Trail Source Scheduler
105. lanking A blank is required when lamps are turned on or certain parameters change see Blanking Method for more information In these cases the instrument will remind the user with the message BLANK REQUIRED Insert an empty cuvette or a cuvette with a blank solution in the cell holder at the measurement position and close the sample compartment ol 22 DU 800 Spectrophotometer Installation amp Operating Instructions Now click the BLANK button The Status amp Control Frame displays Blanking during the blanking and Last blanked at 11 13 AM when it is complete In this example a single blank reading is taken at 260 0 nm The system is now ready to provide accurate readings Reading Insert a cuvette containing a sample into the Single Cell Holder or the appropriate position s of the Multi Position Cell Holder and close the sample compartment The Sample ID for the current sample Sample 1 1 is a default and is shown in blue With a Multi Position Cell Holder there would be a set of default Sample ID s e g from Sample 1 1 to Sample 1 6 You can modify those Sample ID s that are shown in blue by clicking on the respective cell and changing the text followed by the Enter key This must be done before the measurement of the sample s and allows you to use customized Sample ID s for the entire set After a sample reading is complete the respective Sample ID turns black and the cell is locked 60 gt Read Now c
106. lear Loading and saving of a file Saving methods created or modifed Deleting methods Copying methods from another user or to custom applications Backing up and restoring methods Adding and deleting user accounts The file has the fixed name DU 800 Audit Trail log and is a protected file The master System Audit Trail file is kept in the Audit Trail directory located in the INSTALL directory A slave System Audit Trail is maintained in the DATA directory to facilitate data archiving This slave file is automatically updated each time a DU 800 data file is saved To keep the file at a manageable size the operator will be prompted to inform the Administrator that the System Audit Trail requires maintenance The message appears when the entered backup time of file size has been exceeded whichever comes first The range for the backup time is 0 to 2000 days and the range for the file size is 64 to 2048 Kbytes re mo Boop 89 DU 800 Spectrophotometer Installation amp Operating Instructions User Account Manager The User Account Manager allows the administrator to add or delete user accounts and provide the initial password to an account Figure 59 User Account Manager Screen User Account Manager x User Name Add MUA m E Administrator Ron Steiner M O E Philip Pappas O mj O Change Password Delete Close Help The administrator is able to create Add and Dele
107. lications menu item allows the administrator to remove custom applications The menu item is disabled when a custom applications is selected To perform this function a regular application must be selected The list box displays the current custom applications and lets the administrator select one or multiple methods with the usual Windows key combinations Figure 60 Delete Custom Applications Screen Delete Custom Applications Select All Initially the Delete button is disabled It will be enabled when one or more items are selected The deleted custom applications are removed permanently 91 DU 800 Spectrophotometer Installation amp Operating Instructions View System Audit Trail Figure 61 The System Audit Trail menu item opens a new window and displays the content of the active audit trail file or a selected audit trail file The file may be printed or backed up System Audit Trail Screen Audit Trail x March 27 2002 9 49 AM Administrator Load file C DU800 Data Administrator Wavelength Scan test1 dux March 27 2002 9 49 AM Administrator Start of data acquisition March 27 2002 9 49 AM Administrator Data acquisition aborted March 27 2002 9 50 AM Administrator Add user mwong March 27 2002 9 50 4M myvong Logon March 27 2002 9 50 AM Imwong Save method Test Method FVYL March 27 2002 9 50 AM myvong Save file C DU800 Dataynwong Fixed VY
108. lick on the READ button to take a reading After the reading has been taken the result is reported in the data grid and the system is now ready to process the next sample or sample set Open the sample compartment replace the cell with the next sample or sample set when using a multi position cell holder and then close the sample compartment to read the next sample Your window should now look like the one shown below 23 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 17 Window with Fixed Wavelength Application and Measurement Taken From Three Samples Using a Single Cell Holder fly DU 800 UY Yis Spectrophotometer DNA Group USBO01 jol xj System Applications Methods Tools Diagnostic Help Fixed Wavelengtt MA RediRead Ai Cell Match gt Open File Accessories D O aves ere i ae Default Method VA RediScan l Clear fed Save File Edit Method 4 Method Parameter Value File Help Sampling Device Holder Holder Type Single Cell Number of Cells 1 Read Mode Abs Abs 200 0 nm Calculation Mode None of Wavelengths 1 0 5534 Wavelength 1 200 0 nm Read Average Time 0 5 sec Abs a Sample ID 200 0 nm Sample 1 1 0 7255 Sample 2 1 0 5534 Sample 3 1 DU 800 WA E lt Generic User gt Last Blanked at 03 38 PM UY Lamp On for 1 min 18 sec Visible UV Single Cell Holder El Cell Match OFF 600 0 nm 0 3084bs
109. ll Holder g Position Cell Holder 12 Position Cell Holder Tm Microcell Holder Pos mm Pos mm Pos mm _ Custom Cell Holder TH w M Cancel Help The current Cell Holder must be defined by the user Based on the Transport the compatible cell holder items are enabled and the installed cell holder can be selected For ezample a multi position cell holder can only be selected if a transport is installed or has been selected The Tm Microcell Holder can only be selected if the High Performance Transport is installed and selected See Sampling Accessories Cell Holders for additional information With the selection of the Custom Cell Holder the custom cell holder frame becomes visible and the position for each cell location can be defined in millimeters A custom cell holder can have a maximum of 12 positions The cell locations must be within the range of 0 to 112 mm 54 DU 800 Spectrophotometer Installation amp Operating Instructions The Manual Position Control determines the cell position in mm The Enter key or the Apply button will execute the command and move the transport to the specified position The input range is from 0 to 112 mm Sipper The Sipper Accessory uses a peristaltic pump to aspirate the sample into a flow cell for measurement in the DU 800 The sipper is used to semi automate the task of measuring a large number of samples The Sipper Installed item must be check
110. lled cell holders are plugged into the temperature connector on the left sample compartment wall Refer also to the Installation Instructions Peltier Temperature Controller P N 512872 Standard Controller The standard Peltier Temperature Controller is an accessory used with the temperature controlled versions of the Single Cell and Multicell Holders to control the temperature of the sample s in the range of 20 to 40 C The standard Peltier Temperature Controller is not compatible with the Experimental Tm Analysis application High Performance Controller The High Performance Peltier Temperature Controller may be used instead of the standard controller for all cell holders 124 DU 800 Spectrophotometer Installation amp Operating Instructions The Experimental Tm Analysis application requires the High Performance Temperature Controller and the Tm Microcell Holder This combination allows precise temperature control and fast ramping in the range of 0 to 110 C The temperature settings are done in the Experimental Tm Analysis program instead of the Temp Controller tab 125 DU 800 Spectrophotometer Installation amp Operating Instructions 126 DU 800 Spectrophotometer Installation amp Operating Instructions Technical Specifications WA AA Storage and Transport The power should be turned off on the DU 800 Spectrophotometer when not in use for several days or more The sample compartment cove
111. mance Validation Shea aii iia reinas 94 Backup Export System da 96 Restor Import Syst M arene na A E EE 96 Add Siwa O OON HUNA 98 Create Backup License File a n E oda 99 Security ENMANCE IME Nise yssh AN 100 Lock Redi Read and REIS CA A wima 100 Force Users to Save Data wa 101 D ta Me thods Security ai 102 Disallow Creation of Methods by User oooocoococcoocccooccccnonccononanonncconanannnno 102 Show Only Custom Applications ooooooccoccccnonccononccononaconnncnonnnccnnnncnnnnncnnns 102 Data Directory Sttuctire EE 103 Enhancements AA 105 Automatically Convert to CSV File ceeeeeescceceececesececsseeeesneeeenaeeees 105 Diasnostic Menu seheri Seiten nets a a ae Sate WA Wa ia 106 System AU dais tias 106 USEMI ts lia 107 Calibrate Wave Si i a a WAA 107 Run Baseline Testi io da 108 Set SCAM Ga iaa dalt E 108 Reset SOURCE HOUSE setae 110 Seryice DINOS aiii 111 Calibration Dioses a e a se genvsaesacanadeugens Salonen E Eaa 111 DU 800 Spectrophotometer Installation amp Operating Instructions Wave lene Diye RUN sats 1s cence tavaedtaseadseptude HA 112 DRP SA dd 113 Blocked Beam Nos dao 114 Erase EEPRO Misc ironia iii idos 114 Help MEU rs 115 Applications O Ol LoS 116 TALIS ayo WAPAA AAA AA aencoagae tees aura dash hat eer atetsuns E 117 Col Hold rsS lt A eck ie A aah i eae 118 Sipp r ACCESSOLY ww 119 A CONES iea e cast ates cha E T AE ea aha 119 Bateh Sampler ACCESO iia 121 Beckman Coulter ISCO Batch Sampler oo
112. min Auto Print No Auto Save No Filename Prefix Default DU 800 YA aa lt Generic User gt BLANK REQUIRED Visible UY Lamp On for 1 min 19 sec UV Single Cell Holder Ready 300 400 500 Wavelength in nm 600 700 Scan Position Use Name Description q7 CellMatchOFF 600 0nm 0 308Abs 24 8C 13Now2000 10 53AM y You are now ready to take wavelength scans from 200 0 to 800 0 nm at 1200 nm min But HALT before we take the first reading we want to change some parameters which is done in the Method window Editing a Method The lt Default Method gt for Wavelength Scan provides a set of pre defined parameters For example the wavelength range is specified from 200 0 to 800 0 nm and the scan speed at 1200 nm min IS Edit Method We want to change these parameter to fit our requirements Click on the Create Edit Method menu item or the Edit Method icon button This brings up the Method window with the default parameters for the Wavelength Scan application We want to change the Start Wavelength to 500 0 nm leave the End Wavelength at 800 0 nm and then set the Scan Speed to 2400 nm min This new scan speed allows us to scan faster but at the expense of the scan resolution Instead of a 1 0 nm interval at 1200 nm min the data acquisition for a scan at a speed of 2400 nm min is performed with a 2 0 nm interval 26 DU 800 Spectrophotometer Installation amp Operati
113. nction Parameters of the lt Default Method gt can be modified and used temporarily but are not saved The lt Default Method gt provides the initial parameters when creating methods To save selected parameters the method has to be saved under a new name Select Method This item is disabled when there are no methods Only methods that belong to the current user lt Generic User gt logged on user or administrator will be available The available methods are listed and can be selected in the Select Method sub menu The current method name is checked The methods drop down list box in the toolbar provides a shortcut for selecting a method The first item in the list is always lt Default Method gt followed by the available methods 61 DU 800 Spectrophotometer Installation amp Operating Instructions Create Edit Method OS Edit Method This item opens the Method window for the current application and shows the method name and its parameters The icon button Edit Method in the toolbar is a shortcut for this function Method for Fixed Wavelength lt Default Method gt Application Sampler Headers Into Method for Fixed Wavelength Name My Method Application Sampler Headers Info Method for Fixed Wavelength My Method Application Sampler Headers Info There are multiple tabs for the selection of method parameters The tabs and the content of the tabs depend on the current appl
114. nd scan taken as part of the Blank procedure is stored in the instrument and can be reused for an unlimited number of sample scans as long as the range and scan speed remain the same The range can be decreased as long as the scan speed remains constant and no blank is required When a new blank is required it is indicated in the Status amp Control Frame A new blank scan should be made every time a solvent is changed because the blank spectrum will likely be different A new blank scan should also be made if no scan has been taken for over an hour To re scan the blank click on the BLANK icon button while in Wavelength Scan The selected scanning speed determines the distance between each data point that is collected as the instrument scans through the chosen region At 1200 nm min a data point is collected every nanometer At 600 nm min a data point is collected every half nanometer As the sample data are collected the blank is subtracted and the difference in absorbance or transmittance is reported Read Average Time The noise level of the instrument and therefore the uncertainty of a sample reading is decreased by taking a number of readings and averaging them The instrument takes a reading every 0 05 second It takes a series of these readings over a user selected time and averages them to obtain the blank and sample readings For example with a read average time of 0 5 seconds ten readings are taken and averaged The oper
115. ng Instructions First change the Start Wavelength to 500 0 nm Then change the scan speed to 2400 nm min Select 2400 from the Scan Speed drop down list box The Interval value will be recalculated and should now show 2 0 nm as in the following window Figure 19 Method Window for Wavelength Scan After the Change xi lt Default Method gt Application Modes Sampler Report Info m Scan Mode Wavelength Parameters Default me Start Wavelength 500 0 nm 9 agate End Wavelength 800 0 nm Scan Speed 2400 m min Wavelength Interval 20 nm Other Scans per Sample 1 Interval Time 50 0 Seconds y Save As OK Cancel Help In this ezample we will use the modified default parameters temporarily To do so we confirm the changes by clicking on the OK button In this case the selected parameters remain only valid until you leave the Wavelength Scan application or select a new method You may save the modified default method parameters permanently under an appropriate method name by clicking on the Save As button In this case the next time you use the Wavelength Scan application just select your saved method from the Method menu or the method drop down list box and you are ready to go using your individual parameters You may change your method parameters at a later time or save the method under a different name We have now set up the Wavelength Scan application in a way t
116. ng straight up Rotate the source mounting bracket so that the visible source is accessible Figure 106 Removing Visible Source Carefully remove the old source by pulling it straight out Unpackage the new source being careful not to touch the glass envelope on the source Hold the source with a tissue or Kim Wipe while inserting it into the source mount in the same location as the old source 137 DU 800 Spectrophotometer Installation amp Operating Instructions NOTICE Do not touch the glass envelope on the new source If it is touched clean with alcohol and a soft cloth Figure 107 Installing New Visible Source 6 Replace the source mounting bracket and tighten the two thumbscrews Part Il Peaking the Source Tool required Peaking tool provided in the shipping kit ie Make sure the instrument is turned on and has been initialized by the DU 800 System and Applications Software Turn the Visible Source ON Select the Kinetics Time applikcation and choose the lt Default Method gt Click the Edit Method button and change the Interval time to 1 5 seconds then click OK Click on the Blank icon button to blank the instrument on air and the click on the Read button A reading of approximately 0 Abs is displayed as data is collected To peak the source slightly loosen the two thumbscrews that secure the visible source mounting bracket 138 DU 800 Spectrophotometer Installation amp
117. nk using FILL BLANK This will blank the system Flush the solvent from the flow cell using FLUSH or return the solvent using RETURN It is important to have at least a 5 second air flush between each sample to minimize carryover Analyses requiring a re read of standards only Aspirate the standard using FILL READ Then put the focus onto the desired Standard and Replicate by clicking on the respective row in the grid below the standard curve Flush or return each sample Samples If desired change the default sample identification Sample n to a meaningful description Aspirate and read each sample using FILL READ Flush or return after each sample When the analysis is complete clean the flow cell using solvent then an alkaline solution and distilled water When the flow cell is clean leave it filled with distilled water It is important that the flow cell never be left to air dry At the end of the day the daily cleaning procedure described in the Section Cleaning of Flow Cells in the Installation Instructions Single Cell Holders P N 512874 and Installation Instructions Multicell Holders P N 512875 should be used Shortcuts in the cleaning procedure usually prove to be self defeating and should be avoided since a build up of contaminants may occur The tubing should remain attached to the flow cell When use is resumed the flow cell need only be emptied flushed several times then filled with sample 120 DU
118. nonccooncnononcnononcnnnnncncnnnncnnnnannnnnos 60 Custom Applications 61 Methods Men A A idos 61 Default Methods iia 61 Select Method ii A A 61 Create Edit MAN cansa lio 62 Delete Current Method diia iii 63 Copy Methods from Other Users yiscicsecacssdss sackassesdavasccetnacesntecsavacauesdassanrasveeae 63 Copy to Custom Applications it 63 Backup EXPO Methods O a 65 Restore Import Method si isccsscsssscisdsiaceiavedeasscgssades sensdeensdeseiecaed sy caenseedcanasdsnane 65 To ls MAA aaa 67 RM Ri ii tats 68 REIS Car ro is 70 FUNCION Med A ds 72 AAA A E E E ES 72 An TO a E 72 FENO EE KAA SA MANA AAA MAYI catia AOA SANA ONA WANUNUA ATE 73 AS 73 ARIS MSDS dci inn 74 Set Scan MS aida 74 Autocad ir o tala e aag 74 Dynamic Autoscaling a scaiat 74 Read Display Mode nin isa 75 Cell Matehin ge a ad condo 75 DARE e 77 BEI Sy TET ALLIS IA RR 78 2 CFR Pa Aa 78 View Electronic AU A ii 78 Add Electronic Ia don arouse pdnasteees auecge docs aitas 79 User Opinion 80 Q tp t Tabus AO 81 DU 800 Spectrophotometer Installation amp Operating Instructions Halia ER 81 Graph A a e E E T RAEE A E R TEE 81 Colors RID A ee eae oo Me a 81 AMIS INN aha 81 SAAD OG OTIS sp a SIA ANA MAANA NAKAA AAA TANG ee asec NESI 82 Spa UU AAA WA IA EA IA 82 Q tput IA ka 84 SASS EE 85 Graph Tabi ii Na OS USE iN AA E ER 86 Colors e A 87 RE WA HA 88 User Account Mana wak 90 Delete Custom App Uni ii E E 91 View System AU Taide 92 NO 93 Perfor
119. nt Setup Headers Print To print the acquired data select Print from the File menu in the Applications Frame 5 Save File Save To save the acquired data select Save As from the File menu in the Applications Frame or click on the Save File icon button The Save File dialog appears which lets you enter an appropriate filename Click on the OK button to save the data or Cancel to abort If you don t change the target directory the data file DUX is saved to the following location C DU800 Data Generic User Wavelength Scan lt Filename gt dux if the administrator has not changed the default DATA root directory previously NE Clear Clear To end the current task and reset the application e g for another task or to leave the application select Clear from the File menu in the Applications Frame or click on the Clear icon button A message will inform you if the data has not been saved The data grid and the graph are cleared any unsaved data is discarded and the application is reset You may then start another Wavelength Scan task or select another application Performing a Kinetic Run All applications except Wavelength Scan take single wavelength readings in one form or another A Kinetics Run observes the absorbance of a single wavelength from a sample and uses the change of the absorbance over time to calculate a rate usually expressed as dAbs min or dA min Make sure that the Kinetics Time applicati
120. nt Status Bar The status bar is located at the bottom of the screen It displays the status of the operation and the key spectrophotometer parameters The status bar is fixed and cannot be removed Ready Cell Match OFF 600 0nm 0 3084bs 248C 10Jul2001 09 38 AM Operation and Status Message The left field in the status bar is the operation and status message which indicates the current status and or operation e g Scanning cell 4 Spectrophotometer Status The sunken labels represent spectrophotometer status information which are cell match current wavelength of the drive current Abs or T current temperature of the Temperature Controller if any With no Peltier Temperature Controller N A is displayed 51 DU 800 Spectrophotometer Installation amp Operating Instructions System Menu Change User Change Password Log Off v Visible Source v UY Source Accessories Exit User Logon Change User The lt Generic User gt which is the default is not considered a logged on user Therefore in the lt Generic User gt mode the menu item reads User Logon When logged on as a user or administrator the caption changes to Change User and the Change Password and the Log Off items become enabled Figure 31 User Logon Screen r Select pour profile Password Username Administrator X The Logon User and Change User items perform id
121. nt below the specified maximum temperature of 40 C 104 F for adequate cooling For the optical components to maintain their quality it is necessary to operate the instrument in a dust free non corrosive environment If it is required that the instrument be used in a dusty or corrosive environment the optical surfaces must be cleaned or replaced periodically Contact Beckman Coulter Service for more information One component of the instrument that can be user serviced to prevent an interruption in operation is the UV source lamp With time its output gradually decreases close to one half of the original value in approximately 1000 hours At this point in time it is appropriate to replace the UV source even though it is not burned out The length of time that the UV source has been illuminated is shown in the System Status window accessible in the Diagnostics menu A burned out UV source is indicated by the message UV LAMP Failure Instructions for replacing the UV source can be found in UV Source Replacement If decreased sensitivity is seen in the visible range the visible source should be examined to see if a coating has built up on the inside of the glass envelope If so the lamp should be replaced Instructions for replacing the visible source can be found in Visible Source Replacement 129 DU 800 Spectrophotometer Installation amp Operating Instructions System Status Figure 97 The System Status windo
122. nts on or off By default this option is checked The Emphasize Selected Scan option emphasizes the scan in focus with a bold line By default this option is checked The Dynamic Autoscaling option determines if dynamic autoscaling during data acquisition is performed or not By default this option is checked The Y Axis Default values may be modified The default values are 0 0 to 3 0 for Abs input range is 0 3 to 4 5 and 0 to 100 for T input range is 0 to 200 The 3 D Offset frame includes the selection for the data shift to accomplish a 3 dimensional look for overlayed scans The axis values apply to the first scan only All other scans are shifted and the axis values cannot be applied The default is Medium The Default button resets the parameters of this tab 86 DU 800 Spectrophotometer Installation amp Operating Instructions Colors Tab The Colors tab includes the color preferences for the graph window Figure 56 User Options Screen with Colors Tab Selected User Options for Ron Steiner lx Output Headers Graph Colors M Graph Colors gt Default Window Background E Graph Background o Grid Scan Colors 7 UN 2 i o D 3 B s E 4 10 MN 5 i 11 MN amp i 12 uu This tab specifies the Graph Colors for both the screen and the printer The colors are selected by clicking on the colored box This evokes the Windows Dialog function for the selection of
123. o fit our needs by modifying the method in this case the lt Default Method gt The current parameter settings are reflected in the Method Parameters Frame 27 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 20 Method Parameters Frame After the Change Method Parameter Value Sampling Device Holder Holder Type Single Cell Number of Cells 1 Scan Mode Abs Start Wavelength 500 0 nm End Wavelength 800 0 nm Wavelength Interval 2 0 nm Scan Speed 2400 nm min Auto Print No Auto Save No Filename Prefix Default The Method Parameters Frame has been updated when we closed the Method window Notice that it now shows the Start Wavelength with 500 0 nm the Wavelength Interval with 2 0 nm and the Scan Speed with 2400 nm min We are now ready to scan our samples using customized parameters But before we take a scan of the first sample we need to blank the system with the selected wavelength range Blanking A blank is required when lamps are turned on or certain parameters change see Blanking Method for more information In these cases the instrument will remind the user with the message BLANK REQUIRED Insert an empty cuvette or a cuvette with a blank solution in the cell holder at the measurement position and close the sample compartment o Now click the BLANK icon button The Status amp Control Frame displays Blanking when a blank is taken and Last
124. on Scheduler allows the administrator to automatically execute one or more Performance Validation programs at a given day and time Figure 63 Performance Validation Scheduler Screen Performance Yalidation Scheduler x Start Time Program or Group Sunday OFF E None o Monday OFF hu none Tuesday OFF be None v Wednesday OFF none Thursday fos 00 00 Pm Run Group 1 Tests 3 min 30 sec y Friday OFF hae none Saturday OFF i None Results M Print MV Save Results File Prefix Pv Test Default Help ox Cancel The Start Time can be turned OFF or selected in the drop down list box for each day The times are from 5 00 AM to 10 00 PM in Y hour intervals The time format is based on the international setting of Windows The Performance Validation Scheduler will not execute the selected test s when the system is in an open run or a file has been opened The Program or Group selections for the Performance Validation programs are None Run Group 1 Tests 3 min 30 sec Run All Tests 1 hour 4 min 1 Wavelength Drive Test 24 sec 1 Resolution Test 12 sec 1 Baseline Flatness Test 1 min 40 sec 1 Noise Test 1 min 2 Stability Test 60 min BOTH THE VISIBLE AND THE UV LAMPS ARE REQUIRED FOR THE PERFORMANCE VALIDATION SCHEDULER THE SYSTEM WILL MAKE SURE THAT BOTH LAMPS ARE TURNED ON FOR THE TEST S 50 mo fo oS Sp 94 DU 800 Spectrophotometer Installation
125. on is selected and that the current method is the lt Default Method gt by checking the drop down list boxes in the Toolbar If not 30 DU 800 Spectrophotometer Installation amp Operating Instructions click on the down arrow of the drop down list box and select the appropriate item You should see a window similar to the one shown below Figure 22 Window with Kinetics Time Application Selected ka DU 800 U is Spectrophotometer DNA Group USBO01 S 0 xj System Applications Methods Tools Diagnostic Help Kinetics Time T do E MA RediRead fill Cell Match 5 OpenFile mat Accessories Gror lt Default Method gt J Th Redi Scan Clear LA SaveFile Edit Method i Method Parameter Value File Mode Function Axis Help Data Acquisition Sampling Device Holder Holder Type Single Cell Number of Cells 1 Sample Assignment E Analytic Wavelength 540 0 nm Bkg Wavelength 250 0 nm Bkg Correction No Interval Time 15 0 sec Total Time 120 0 sec Factor 1 0000 Units ng ml Temperature 37 0 Celsius DU 800 O fa 0 20 40 60 80 100 120 COULTER iaa Time in Seconds lt Generic User gt BLANK REQUIRED Tea Sample 1 Sample2 Sample3 Sample 4 Sample 5 Sample 6 Visible UV Single Cell Holder Ready CellMatchOFF 600 0nm 0 308Abs 248C 20Jul2001 1253PM 7 You are now ready to start the Kinetic run But HALT before we take the first
126. ontaminants may occur The tubing should remain attached to the flow cell When use is resumed the flow cell need only be emptied flushed several times then filled with sample Manual Operation It is sometimes desirable or necessary to place samples in the batch sampler and manually aspirate them into the instrument for readings rather than analyzing them automatically Examples of situations where this may occur are Blanking before an automatic analysis 2 After automatic analysis of standards when one or more standards need to be re run 3 When the batch sampler is set up and used for a routine assay Between assays a wavelength scan needs to be taken on a sample To perform these analyses 1 Verify that the desired operating parameters are set In the third example above the RediScan mode can be used Place the sample in the batch sampler under the aspirator arm Click on the Lower Arm button to lower the aspirator arm Press FLUSH until the flow cell is filled to aspirate the solution manually Blank or take a reading After the reading is taken press FLUSH to aspirate the solution to waste PD E A te To read another solution located in the next position in the batch sampler click on the appropriate button to raise the aspirator arm advance to the next sample and lower the arm again Repeat the directions in steps 4 to 6 to take another reading Peltier Temperature Controller NOTICE To control the s
127. ontents Search by Index System Software Applications Software Support and Service Installation Getting Started Accessories d Adobe Acrobat Files PDF gt Installation and Operating Instructions Software Reference Manual Applications See proa Installation Instructions For Accessories Transport En a Single Cell Holders Certificate of Compliance y e Multicell Holders Software Validation Pack y Sipper Accessory Warranty Registration Batch Sampler Peltier Temperature Controller Contents Search by Index System Software Applications Software Support and Service Installation Getting Started Accessories Transport Adobe Acrobat Files PDF gt zzl aeie Sipper Accessory About this Program Batch Sampler Peltier Temperature Controller The first three sections of this menu allow the user to access the help file in different ways The Contents item calls the List of Topics and lets you go to a specific item systematically The Search by Index item lets you search for a specific item by index The System Software Applications Software and Support and Service items will bring you right to the top level of the respective section The Installation and Getting Started items should be accessed when installing the system and to introduce a user to the basic functionality using three examples The Accessories item gets you right to the appropriate accessory section The Adobe Acrobat F
128. ooconnnococnoncccnonccononcnononcconnncn nnncnnns 121 Automatic Opa a e A e E TR 122 Manual Operation essensen ia ps 123 Peltier Temperature Controller ois aiii 123 Standard Con caes 124 High Performance Controle iria 124 storage and VANS PORE li ita 127 Sample Compartment Configuration cooocononoccnonoccnoncncnnnnnonnncnonnnnnnnnnncnnnnnnonncnnnnnos 127 P ttormance PE Ca ii 128 Physical amp Environmental Specifications cc eecesseesecsseceseeeseeesaeeesaeenseensees 128 GEnera PLOT E E E CIR ET 129 System Sal iii 130 ES RA e bon Loros lead O 130 UV So rce REP nit 132 Visible Source Replacement isa 135 Part I Replacing the QUITO vna iaa 135 Part Il Peakmne tne UI cae 138 Startup Diagnostic 141 Operational Sai Eia E a EE E E E E T 142 Operational Messages wi ia E 143 Warranty Registrations INI 145 Beckman Coulter OCES sr erens sitas 146 North America scssi ad 146 United Stales sce ee aia i a R e R a SN 146 LG aa Ta e ita II adassaet KAWI WAA 146 Lati AMECA az 146 Mekito O O 146 DU 800 Spectrophotometer Installation amp Operating Instructions PLUGS IG AI AL ae 147 PTAC olaa 147 German AA ae ae 147 MA O 147 Netherlands ii ai 147 SPAM tees oes AA 148 BW CC Meco AA AA 148 Switzerland hore aas A ON 148 A a e as 148 United RIN BOG ieser E E N 148 ATA PACING AA ma 149 PUSAN A A i n 149 O A O 149 A E 149 A O 149 SIDO a ia 150 VA as 150 Eastern Europe Middle East Africa 3 2 i4 c4s siccassiaheaicd
129. ow data are acquired by limiting the system to Custom Applications which are actually customized methods from the standard and purchased optional applications In this case the regular applications provided by Beckman Coulter will not show and can therefore not be accessed This setting will have no affect on administrators 102 DU 800 Spectrophotometer Installation amp Operating Instructions The check box for this feature is located in the Data Methods Security tab of the Security Enhancements item in the Administration menu By default the parameter Show Only Custom Applications is unchecked When checked the standard and purchased optional applications that have been provided by Beckman Coulter will be hidden and only Custom Applications can be accessed by a user ATTENTION When the feature is activated and the system cannot find a custom application Fixed Wavelength is selected automatically as the application and the message No cusfom applications Please create one appears In this case the administrator must first create a custom method and copy it to Custom Applications Please shut down and re start the DU 800 System amp Applications Software for the feature to take effect Data Directory Structure The window with the Data Directory Structure tab can be accessed from the Administration menu Figure 75 Data Directory Structure x 21 CFR Part 11 Compliance Enhancemen
130. photometer Installation amp Operating Instructions Backup Export Methods This function allows a user to backup one or multiple methods from the current user to a file It can also be used to physically move one or multiple methods from one instrument to another The lt Default Method gt is a fixed method and is not listed Figure 40 Backup Export Methods Screen Backup Export Methods from Philip Pappas x Method Name Created On Application DNA Ratio 260 265 06 June 2001 Fixed Wavelength Kinetics Example 06 June 2001 Kinetic Time Drive Select All Backup Export Close Help One or more methods may be selected and backed up by clicking on the Backup Export button The button is enabled when one or more items are selected Backup files have the extension bkx The initial backup directory is the Backup Files folder in the DATA directory e g C DU800 Data Steiner Backup Files It will be created automatically if it does not already exist Restore Import Methods This function restores all or selected methods from a backup file The initial backup directory is the Backup Files folder in the DATA directory e g C DU800 Data Steiner Backup Files However the user may browse and select a backup file from another location e g Drive A 65 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 41 Restore Import Methods Screen Restore Import Methods from Philip Pappa
131. pler Select the available batch sampler or None Pump Parameters The parameters Fill Time Return Time Read Delay Time and Speed are sipper parameters and are described in the Sipper Tab Wash Time If a wash between samples is selected input the time for the wash 56 DU 800 Spectrophotometer Installation amp Operating Instructions Wash Between Samples If the batch sampler has a wash station a wash between each sample can be selected by checking the box Return Mode Select the desired Return Mode by clicking on the respective radio button The percentage values are that of the sipper fill time The return modes have the following uses and limitations Waste Sample Flushes the sample to waste for 5 seconds No Return Raise arm Flush 5 seconds Forward Flush Returns most of the sample with the aspirator arm lowered raises the aspirator arm to finish returning the sample then flushes to waste to remove the maximum amount of sample from the tubing 80 Return Raise arm Wait 1 second Return 20 Wait 1 second Return 20 Wait 1 second Flush 100 Forward Flush will not aerate the sample but has the possibility of creating aerosols and does send the sample through the pump tubing Reverse Flush Returns all of the sample with the aspirator arm lowered then raises the aspirator arm and attempts to remove the maximum amount of sample from the tubing 200 Return Rai
132. plications menu or a drop down list box When selecting or initializing an application the lt Default Method gt will be applied and the Applications Area which includes the Applications Menu Bar and the Data Scan Area will reflect the functionality of the application as described in this section 43 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 29 Integrated Frame Oriented User Interface Main Menu System Applications Methods Tools Diagnostic Help MA RediRead fill Cell Match gt Open File ext Accessorie BLK Read 3 Fo Th RediScan Clear E Save File El Edit Method Method Parameter File Help Sampling Device Holder Type Number of Cells Read Mode Calculation Mode Abs 200 0 nm Abs 250 0 nm Abs 300 0 nm Hof Wavelengths j 0 9820 0 8914 0 0762 Wavelength 1 Wavelength 2 Wavelength 3 Read Average Time _ Sape 200 0nm 250 0rm 300 0 nm Applications Sample 1 1 Frame Parameters Sample 1 2 Sample 1 3 Frame Sample 1 4 Sample 1 5 Sample 1 6 Sample 2 1 Sample 2 2 lt Generic User gt Sample 2 3 Last Blanked at 09 38 AM Sample 2 4 UV Lamp On for 43 sec Sample 2 5 Sample 2 6 Visible UV Status amp Control S3lifalalalolo Frame Ready CellMatchOFF 6000nm O308Abs 2480 10Jul2001 09 38AM Status Bar Some commands and functions such as Blank Read Stop Save File Open File etc apply to all
133. ppropriate action The operator may click on the Abort button to end the system initialization Figure 28 DU 800 System Initialization Screen DU 800 System Initialization x A COULTER System Software Download Complete Building WL Calibration Table Complete m Startup Diagnostic Tests Gain Passed Visible Lamp Passed Light Path Passed Shutter Passed Filter Passed Wavelength Drive In Progress Detector EEPROM CTT Abort Continue Enint The Print button which can be used to create a startup diagnostic report becomes enabled after the system has been initialized To continue and operate the software press Enter or click the Continue button after the Startup Diagnostic Tests have been completed If the software detects a spectrophotometer but cannot establish communication after the firmware has been downloaded the user will be alerted by the message Communication with the DU 800 UV Vis Spectrophotometer could not be established In this case the software continues but without initializing the spectrophotometer Off Line Launch Check the connection or see Section 7 Troubleshooting If the software detects a spectrophotometer on the USB port the DU 800 System will be initialized automatically if required 42 DU 800 Spectrophotometer Installation amp Operating Instructions After the system has been initialized and communication with the spectrophotometer is lost
134. r should be closed It is necessary that the instrument remain in the upright position on a flat surface so that the optics will remain stable The instrument should be transported in the upright position with care taken not to jolt bounce or shake the instrument during transport Pack the instrument in the original shipping container if still available Sample Compartment Configuration The following diagram shows the location and size of the beam in the sample compartment Notice that the beam focus is near the right hand side of the sample compartment Sampling accessories are installed in this location and will position the sample at the beam focus Figure 96 Diagram of Location and Size of the Beam in Sample Compartment 25 14 MM ACCESSORY MOUNTING LOCATION 35 56 MM 74 04 MM t 6 35 MM FRONT VIEW 38 48 MM 127 DU 800 Spectrophotometer Installation amp Operating Instructions Performance Specifications Scan Speeds 120 240 600 1200 2400 nm min Data Collection Rate 20 Samplings per Second Response Time 0 05 Seconds Wavelength Range 190 to 1100 nm Wavelength Setability 0 1 nm Increments Wavelength Accuracy At 656 1 nm 0 2 nm Full Range 0 5 nm Wavelength Repeatability At 656 1 nm 0 1 nm Full Range 0 2 nm Spectral Bandwidth from 200 to 680 nm lt 1 8 nm Photometric Readout 0 300 to 3 000A or 0 0 to 200 T Photometric Accurac
135. reading we want to change some parameters which is done in the Method window Editing a Method The lt Default Method gt for Kinetics Time provides a set of pre defined parameters For example in the lt Default Method gt the analytical wavelength is set to 540 0 nm and samples are supposed to be observed over a total time of 120 seconds taking a measurement for each sample every 15 seconds In addition we want to monitor the rate during the run to see if the absorbance change over time is within the expected range 3 Edit Method We want to change these parameter to fit our requirements Click on the Create Edit Method menu item or the Edit Method icon button This brings up the window shown below with the default parameters for the Kinetics Time application 31 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 23 Method Window for Kinetics Time Before the Change Method for Kinetics Time x lt Default Method gt Application Sampler Report Info Wavelengths _ _ _ _ _ _ _ Default Analytical Wavelength 540 0 nm Background Wavelength 250 0 nm Background Correction Kinetic p Curve Offset Samples 6 None Interval Time 150 Seconds y K aa Total Time 1200 Seconds y z Factor Units 1 0000 ng ml Temperature 370 E Delta Abs Save As OK Cancel Help We want to change the n
136. rly It also assures quick and easy access to the Beckman Coulter Product Support team for answers to your questions Please remove the box located on the right side of the instrument This box is the Shipping Kit Remove any packing material that covers the instrument and carefully place the DU 800 at the chosen location Shipping Kit Please locate the DU 800 Shipping Kit which is packed in the large DU 800 shipping box The Shipping Kit contains the various items required for the operation of the DU 800 Spectrophotometer Verify that the contents of the shipping kit match the items shown on the included list PC Monitor and Optional Printer The DU 800 Spectrophotometer is controlled by the DU 800 System and Applications Software running under Microsoft Windows 2000 The required PC and monitor and or the optional printer may have been purchased from Beckman Coulter or they may have been acquired from another source If the PC was not purchased from Beckman Coulter please verify that it meets the minimum requirements Please refer to the manuals included with the PC monitor and printer for unpacking information 12 DU 800 Spectrophotometer Installation amp Operating Instructions Hardware Installation 1 Place the DU 800 Spectrophotometer in the chosen location and allow access to the back panel until installation is complete Figure9 DU 800 Spectrophotometer Rear Panel Connectors CS o EY At Jo
137. rm then click OK Cancel The first window is the default When clicking on Change Password the window will be enlarged to allow the operator to enter and confirm a new password Three attempts to enter the correct password are allowed These passwords are not case sensitive Calibration Info More information about this function is available in the DU 800 Service Manual Figure 85 Calibration Information Screen Calibration Information X SCAN GAINS Wavelength Gain 190 0 321 0 nm 240 321 1 356 0 nm 255 356 1 415 0 nm 224 415 1 556 0 nm 48 556 1 735 0 nm 24 735 1 1100 0 nm 40 OTHER Grating E 1900 17 Sensor Location 3126 Current Drive Location 6904 D2 Reference Location 6793 Close 111 DU 800 Spectrophotometer Installation amp Operating Instructions Wavelength Drive Run More information about this function is available in the DU 800 Service Manual Figure 86 Wavelength Drive Run Screen Wavelength Drive Run X Cycle wavelength drive from O to 1100 nm Number of Cycles 25 Current Cycle 14 Stop Bun Close Current Cycle X will be displayed during a run and then replaced by Finished Run 112 DU 800 Spectrophotometer Installation amp Operating Instructions DRP Scan More information about this function is available in the DU 800 Service Manual Figure 87 DRP Scan Screen D
138. s may restrict the movement of the sample compartment cover If purchased the batch sampler should be placed adjacent to the right hand side of the instrument so to minimize the length of tubing needed to reach the sample compartment The other accessories can be placed in a convenient location near the instrument within reach of the interconnecting cables The spatial requirements for the DU 800 PC and accessories are diagrammed in the following Figures Dimensions shown in cm and inches The shaded areas show additional space required for air circulation Do not block these air spaces Figure5 Minimum Space Requirements with Desktop PC NN 46 18 gt 43 17 gt pi y Keyboard Keyboard 17 0 h 46 da A 901218L Al Figure6 Minimum Space Requirements with Tower PC p TR CONNECTIONS Las 17 gt tg 38 Printer 5 Keyboard 7 Keyboard ue 46 18 gt 901219L Al DU 800 Spectrophotometer Installation amp Operating Instructions The following Figure shows a configuration with other optional accessories such as Peltier Temperature Controller Sipper Accessory and Batch Sampler Figure 7 Space Requirements with Optional Accessories and Tower PC L 69 a 7 19 8 gt E a gt lt 29 12 gt ia an PE 5 5 t 56 59 49 So Batch FA 22 Sper 2 Keyboard PE em 901217L Al Power Requirements C
139. s x Method Name Created On Application DNA Ratio 260 285 06 June 2001 Fixed Wavelength Kinetics Example 06 June 2001 Kinetic Time Drive Overwrite Existing Methods Select All Restore Import Close Help All methods in the backup file are shown and can be restored or imported One or more methods may be selected and restored by clicking on the Restore Import button which is enabled when one or more items are selected The window features the Overwrite Existing Methods function When this function is unchecked and an identical and existing method name is encountered a sequential number lt method name gt 01 will be appended to the restored method name 66 DU 800 Spectrophotometer Installation amp Operating Instructions Tools Menu Generic User User Logon RediRead RediRead RediScan RediScan Cell Matching Cell Matching Data Export Data Export View Electronic Signaturets View Electronic Sianature s Add Electronic Signature Add Electronic Signature s User Options User Options Administration Administration Administrator Logon RediRead RediScan Cell Matching Data Export View Electronic Signaturets Add Electronic Signature User Options Administration System Options User Account Manager Delete Custom Applications System Audit Trai
140. sdassaeydehcobanatoessceosvea dh 150 HOUR AICS AN A sauces 150 vi DU 800 Spectrophotometer Installation amp Operating Instructions List of Figures A Figure 1 DU 800 Spectrophotometer with Computer and Monitor s s sswmmmmenwa 1 Figure 2Opuiea DI sie iodo ssa 4 Figure 3 DU 800 Spectrophotometer minis lesdaansadvadess 7 Figure 4 DU 800 System and Applications Software ooooconocccoccnnocnconncnonononncnn cnn ncnnnnnnnnnons 8 Figure 5 Minimum Space Requirements with Desktop PC ooooconoccciocococononnconncnoncnannnnnn non 9 Figure 6 Minimum Space Requirements with Tower PC ooooonicccnocccinccconononcnonnconncnnnnnnnnnon 9 Figure 7 Space Requirements with Optional Accessories and Tower PC 10 Figure 8 DU 800 Spectrophotometer on Pallet 2 0 0 eee eeecceceeceeceeeeeceeeeecseeeeenteeeeneeeee 11 Figure 9 DU 800 Spectrophotometer Rear Panel Connectors ssmmmsmmnmnmsa 13 Figure 10 USB Connections on the PC and Spectrophotometer wwwwmmwemewe 14 Figure 11 Found New Hardware Wizard Screen swsemmnmmnmanmanimmamanm amma 16 Figure 12 User Display Screen 23icc cA a ee a IG est 17 Figure 13 Window with Fixed Wavelength Application Selected lt lt lt v 19 Figure 14 Method Window for Fixed Wavelenght Before the Change v 20 Figure 15 Method Window for Fixed Wavelength After the Chan
141. se arm Wait 1 second Return 20 Wait 1 second Return 20 Wait 1 second Return 100 Reverse Flush will not aerate the sample but has the possibility of creating aerosols It does not send the sample through the pump tubing Lower Flush Returns all of the sample with the aspirator arm lowered 200 Return Raise arm 57 DU 800 Spectrophotometer Installation amp Operating Instructions Lower Flush will clean the tubing as well as Reverse Flush but does not create aerosols and does not send the sample through the pump tubing It does aerate the sample In general if return of the sample is desirable Use Forward Flush if aerating bubbling the sample causes problems Use Lower Flush if creating aerosols causes problems Use either Reverse Flush or Lower Flush if the sample will degrade the pump tubing See Accessories Batch Sampler for additional information 58 DU 800 Spectrophotometer Installation amp Operating Instructions Peltier Temperature Controller The Peltier Temperature Controller is used in conjunction with the High Performance Transport and a Peltier Temperature Controlled Cell Holder It cannot operate if these two accessories are not installed The temperature controller is activated by checking the Temperature Controller Enabled box Figure 36 Accessories Screen with Temp Controller Tab Selected Accessories x Transport Holder Sipper Batch Sampl
142. sories The installed accessories must be defined for the system to function properly After making any hardware changes to the DU 800 such as changing the cell holder or installing a sipper the appropriate tab of the accessories window must be updated WA Accessories Shortcut for Accessories For more information on the Accessories setup refer to Transport and Holders Sipper Accessory Batch Sampler 53 DU 800 Spectrophotometer Installation amp Operating Instructions Peltier Temperature Controller Exit Exits the DU 800 System and Applications Software Before exiting the system will prompt the user when data has not been saved Transport Holder Depending on the mode the software automatically detects the presence and type of the Transport The proper selection is made automatically in the Transport Holder tab The user must still select the Holder that is mounted on the transport On Line Mode Transport is detected automatically user cannot select it Off Line Mode Transport is set to None user cannot select it Figure 33 Accessories Screen with Transport Holder Tab Selected Accessories x Transport Holder Sipper Batch Sampler Temp Controller E Transport PS Manual Position Control mm C None 7 il Standard Apply C High Performance m Holder Custom Cell Holder Single Cell Holder Number of Positions 12 y 6 Position Cell Holder 7 Position Ce
143. tation modes to determine molecular weight absorptivity extinction DU 800 Spectrophotometer Installation amp Operating Instructions coefficient concentration and the theoretical melting point for oligonucleotide DNA samples Protein Analysis Calculates protein concentrations using the Bradford Lowry Biuret Direct UV Colloidal Gold or Bicinchoninate BCA methods The user may choose to add delete or re run individual standards based upon the statistical analysis of the standard curve Enzyme Mechanism Provides for rapid and easy characterization of a wide variety of enzyme reactions The software calculates and reports Km Vmax kcat and Ki as well as the Hill constant The following functions are available Michaelis Menten Lineweaver Burk Eadie Hofstee Hanes Woolf Hill and Inhibitor plots Enzyme Activity Calculates the enzyme activity of large numbers of samples from chromatography fractions Experimental Tm Analysis Allows the study of denaturation and renaturation of DNA samples The thermal melting point Tm is determined quickly and efficiently using the First Derivative 2 Point Average or a Non Linear Curve Fit algorithm Up to six micro volume samples with 325 UL can be processed automatically applying up to three temperature ramps A full line of modular accessories is available ambient and temperature controlled single and multi position cell holders sipper accessory and batch sampler Acc
144. te user accounts A user requires a password which is initially given by the administrator From this point on the user is able to change his or her password at any time The Change Password button allows the administrator to change the password of an existing user For each user one or more Electronic Signature Privileges can be assigned The default is Author The privileges include Author Reviewer and Approver For more information on Electronic Signatures refer to Electronic Signature s The minimum number of characters for both user name and password is 4 The maximum length is 32 for the user name and 12 for the password The current users are shown in a list box To change a user s password or to delete the account select the respective user name first To create a user account simply enter the new user name and click on the Add button The Create Password window pops up which lets you enter the initial password Enter a password and click on OK to return to the User Account Manager You may then continue to add user accounts or close the window To delete a user account select the appropriate user name in the list box and click on the Delete button The user name will be removed from the list and the account deleted The methods and data files for this user account will not be deleted 90 DU 800 Spectrophotometer Installation amp Operating Instructions Delete Custom Applications The Delete Custom App
145. tect the process The start of an open run is the measurement of the first sample The run will be closed when the Clear command is given usually after the data has been saved The time in between is defined as an open run For a definition of open run data acquisition and other terms please refer to Terminology 45 DU 800 Spectrophotometer Installation amp Operating Instructions RediRead and RediScan Ria RediRead yy Redi Scan Opens the RediRead or RediScan window which allows the user to take quick readings or scans These icon buttons are shortcuts for the RediRead and RediScan items in the Tools menu Cell Match A Cell Match Opens the Cell Matching window which allows the user to match multiple cells This icon button is a shortcut for the Cell Matching item in the Tools menu Edit Method Opens the Method window for the current application with the currently selected method name and its parameters This icon button is a shortcut for the Create Edit Method item in the Methods menu C2 Edit Method Open File Save File and Clear 2 Open File lke Save File lle Clear The Open File Save File and Clear buttons represent shortcuts for the File menu items of the current application and perform the same function as the respective items Opening a file is not like opening a file in Microsoft Word there are important considerations in this software which are described below To keep the s
146. ting Instructions Source Scheduler The Source Scheduler allows the administrator to turn both sources on and off individually at a scheduled time for specified days Figure 62 Source Scheduler Screen Source Scheduler x Visible Cw On of sad On off Use Monday foz oo 00AmM y fos oo 00PmM y gt fozoo004m y fos 00 00PmM I Tuesday foz oo 00AmM y fos oo 00PmM y gt fo7 o0004M y fos 00 00PmM I Wednesday 07 00 00AM y fos 00 00Pm y Fo fozooo0am y fos oo 00PM I Thursday 07 00 00AM y oe00 00PM y PF fo7 00 004M y os 00 00PM C Friday fo7 00 004M x foeo0 00PM y gt for 00 004M y os 00 00PM I Saturday jor y OFF oF y oF y Default Help OK Cancel OK The default settings are shown in the above screen The Source Scheduler will not work when the system is in an open run or when a file has been opened The On and Off times can be set to OFF or from 5 00 AM to 10 00 PM for each day The times are in 2 hour intervals and the format is based on the international setting of Windows The Use check box provides a quick way to turn the scheduler on or off for a particular day without changing the previously selected times By default these boxes are unchecked The Default button resets the preferences of the Source Scheduler 93 DU 800 Spectrophotometer Installation amp Operating Instructions Performance Validation Scheduler The Performance Validati
147. tions are provided in Visible Source Replacement Something is blocking the light path through the sample compartment Tient path Remove the sampling accessory and re initialize the system Shutter Readings cannot be taken The instrument can be used for other functions Filter Readings cannot be taken The instrument can be used for other functions EEPROM Readings cannot be taken The instrument can be used for other functions The wavelength may not be accurate Use the instrument only if the Wavelength Drive accuracy of the readings can be verified Contact the Beckman Coulter service Operational Failures There are three failures that can occur during instrument operation These are displayed in the Permanent Menu Bar on the left hand side The failure is displayed until the condition is corrected Message Recommended Action VISIBLE LAMP Failure Replace the visible source Directions are given in Visible Source Replacement UV LAMP Failure Replace the UV source Directions are given in UV Source Replacement Visible tamp Replace the visible source Directions are provided in Visible Source Replacement Something is blocking the light path through the sample Light Path compartment Remove the sampling accessory and re initialize the system Filter Readings cannot be taken The instrument can be used for other functions Readings cannot be taken The instrument can be used for oth
148. tom Applications Standard Optional and Custom Applications Applications Applications e Fixed Wavelength e Fixed Wavelength Wavelength Scan Wavelength Scan II Kinetics Time Kinetics Time Single Component Analysis Single Component Analysis Nucleic Acid Analysis Nucleic Acid Analysis II Gees Protein A Analysi Performance Validation paid j ae Enzyme Mechanism Gustom Applications Enzyme Activity Experimental Tm Analysis Performance Yalidation Custom Applications Lab 110 DNA Ratio Beer Analysis Standard and Optional Applications The Application menu provides the user access to the standard applications and any optional applications that are installed There are two types of optional applications Extended Standard Applications e g Wavelength Scan II The standard application Wavelength Scan is replaced by the optional application Wavelength Scan II The optional application expands the functionality of the standard application Stand Alone Applications These application items are added to the menu Standard applications are available immediately after software installation The administrator may add optional applications that have been purchased See Add Software Option s for details When selecting an application all methods belonging to the application will be loaded and can be selected The lt Default Method gt is the initial method The related menu items and the methods list box w
149. tructure clear and simple the following has been implemented a Users cannot select another Application or Method and or change the user during an open run The respective menu items and list boxes are disabled during an open run b The Save File and the Clear items buttons are disabled when no data has been acquired or loaded When data has been acquired or when a file has been loaded the user must press Clear to end the open run or file loaded mode Save File will save the current data but will not close the run Clear will close the open run and reset the application If necessary it will remind the user to save data before clearing c Depending on the application the Open File item button may be disabled during an open run 46 DU 800 Spectrophotometer Installation amp Operating Instructions d All data files are associated with an application When opening a file the associated application is launched automatically if required When a file has been opened the following happens l The methods drop down list displays the filename instead of the method This clearly indicates a file loaded mode instead of the regular read mode The displayed parameters are the file parameters not the method parameters and there is no link to a method name Applications and Methods menu items and list boxes will be disabled The Edit Method icon button is enabled to allow post run analysis It also allows the us
150. ts Data Directory Structure Data Methods Securty Detaut Use Custom Settings Level 1 Subdirectory Default Uses Level 2Subdrectory Default Applicaton The Default directory structure is fixed as Data Root Directory as specified by the administrator User logged on user Application selected application name By default all acquired data will be saved to and accessed from this location e g C DU800 Data Generic User Fixed Wavelength 103 DU 800 Spectrophotometer Installation amp Operating Instructions Alternatively the administrator may set up the system to be using a customized directory structure by clicking on Use Custom Settings In this case the fixed directory structure or tree will be replaced by a more flexible and customizable directory tree This more flexible data directory tree is defined as Data Root Directory as specified by the administrator Application selected application name Product Name or Component Name or Lot Number or whatever the meaning of the appropriate entry is User logged on user optional These parameters are part of the method setup Report tab Figure 76 Data Directory Structure x 21 CFR Part 11 Compliance Enhancements Data Directory Structure Data Methods Securty C Default Use Custom Settings Level 1 Subdrectory Default Uses Level 2Subdrectory Default Application Product Name
151. ts for a number of common functions such as BLANK READ and STOP buttons In addition other common functions can be accessed from the toolbar MA RediRead fill CellMatch E OpenFile tet Accessories BLK Read _ i z e tA RediScan l Clear E Save File 43 Edit Method The top drop down list box in the toolbar contains the Applications and provides the same functionality as the Applications menu item The lower drop down list box in the toolbar contains the Methods and provides the same functionality as some of the Methods menu items BLANK GO READ SCAN and STOP Buttons aux C Read C Scan D Blank Read or Scan and Stop are the most common commands and are executed through large icon buttons Read is used as the caption for the GO button for all applications except Wavelength Scan The Wavelength Scan application uses Scan as the caption for the GO button Blank will blank on one or multiple single wavelengths read blank or a wavelength range scan blank depending on the application During data acquisition the Stop button is enabled and halts the measurement when the current readings for the selected wavelengths have been completed or at the next possible stop point for a scan Complete scan data that have been acquired will be retained and the user makes the choice to save or discard the data During an open run and data acquisition certain controls are disabled and cannot be used in order to pro
152. umber of samples to and correct the measurement for background at 480 0 nm First click on the Background Wavelength field and enter 480 Then check the Background Correction box to determine that a measurement will also be taken at the background wavelength of 480 0 nm in addition to the measurement at the analytical wavelengths The result of the two measurements will be an absorbance corrected for background a so called Net Absorbance absorbance background absorbance Second click on the Samples field in the Kinetic frame and enter 1 This determines that the Kinetic run will consist of a single sample Third change the Interval Time and Total Time to values that better suit this experiment Set the Interval Time to 5 seconds and the Total Time to 60 seconds Finally check the Delta Abs box so we can monitor the rate dAbs min for each time point starting with the second This absorbance change at any time point gives us an indication about the time course of the kinetics and would allow us to make a decision in order to abort the kinetic run if the rate of the absorbance change is not what we expect it to be All parameters have been set and your method window should look like the one shown below 32 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 24 Method Window for Kinetics After the Change x lt Default Method gt Application Sampler Report Info m Wavelengths Default
153. unpacked inspect all parts for shipping damage and if necessary contact the carrier regarding any damage caused in shipment as soon as possible Note that the carrier is responsible for damage occurred while in transit Use the respective packing slips to verify that the listed items of each package were received Report any shortages to the local Beckman Coulter sales office Unpacking Instrument The original shipping container for the DU 800 Spectrophotometer is attached to a pallet If not already removed from the pallet cut the straps that hold the shipping container together Figure 8 DU 800 Spectrophotometer on Pallet DU 800 Package with PC DU 800 Package without PC ECKMAN ECKMAN Sas Osses en a aed 901228L AI Remove the straps and the instrument box which sits on the pallet on top of the instrument and the PC if purchased Locate the Quick Installation Sheet and any other documentation that may be present and put it aside 11 DU 800 Spectrophotometer Installation amp Operating Instructions The Quick Installation Sheet can be used to quickly setup the DU 800 Spectrophotometer in a step by step fashion More information on installation is provided in the DU 800 Installation and Operating Instructions NOTICE Complete and return the Warranty Registration after installation This will guarantee that your system will be registered and that the one year warranty is applied prope
154. ure 103 Reset Source Hours Screen Reset Source Hours X ATTENTION Please DO NOT reset the source hours unless the respective source has been replaced Visible Source 752 3 hours UV Source 1297 1 hours Reset Hours for Visible Source Help Reset Hours for UY Source Close Visible Source Replacement Part I Replacing the Source Parts required Visible Source P N514259 1 Unplug the instrument power cord Allow the instrument to cool for 15 minutes CAUTION To avoid risk of electrical shock disconnect power cord to instrument before changing either source 135 DU 800 Spectrophotometer Installation amp Operating Instructions Attention Resque de choc electrique Debrancher l appareil avant de changer de source The visible lamp operates at a high temperature To prevent burns allow at least 15 minutes for the instrument and sources to cool before handling internal components 2 Locate the source access door on the top left hand of the instrument cover then loosen the thumb screw and open it Figure 104 Location of Source Access Door on DU 800 Sim 136 DU 800 Spectrophotometer Installation amp Operating Instructions 3 Unscrew the two thumbscrews that hold the visible source mounting bracket in position Figure 105 Unscrewing Thumbscrews Holding Visible Source 4 Thumb screws Remove the source mounting bracket by lifti
155. velength s are identical to the wavelength s of the application and that the cell match values are recent and from the same cells in the same position and that the cells have not been removed For the most accurate correction the cell match readings should be taken at the same wavelength s as in the subsequent application and the cuvettes should not be removed from the cell holder before the analysis If the cuvettes are removed it is necessary to return each to the same cell position in the same orientation for the correction to be valid Cell match correction is recommended when using the 6 Position Microcell Holder and the 12 Position Microcell Holder The column labels indicate the wavelength and the matching values in dAbs and d T The number of cells displayed and processed here is based on the current cell holder selection in the Accessories window The maximum number of cells is 12 The selection of a different number of wavelengths or the change of a wavelength will clear the grid and a new match has to be performed 76 DU 800 Spectrophotometer Installation amp Operating Instructions Data Export The Data Export item allows the user to save the currently displayed data as a CSV file The CSV Comma Separated Values format is a universal format and recognized by third party spreadsheet programs such as Microsoft Excel Figure 48 Example of Data Exported as a CSV File File Edit View Insert Format Tools Dat
156. version and copyright information Applications Software The applications software is described in Help and the optional Software Reference Manual 116 DU 800 Spectrophotometer Installation amp Operating Instructions Sampling Accessories WAY Ul Transport Ambient or water temperature controlled Single Cell Holders can be installed on the Static Mount and do not require a transport However they may also be mounted on the transport Multi Position Cell Holders require a transport Figure 91 Transport Accessory A901288P Al Two types of transports are available e Standard Transport For ambient and water temperature controlled cell holders and all types of cuvettes High Performance Transport Reguired for Peltier temperature controlled cell holders This transport contains a fan to remove excess heat and provides additional accuracy in positioning microcells Refer also to the Installation Instructions Transport Accessory P N 512870 117 DU 800 Spectrophotometer Installation amp Operating Instructions Cell Holders The installed cell holder must be specified by the user in the Transport Holders tab of the Accessories window All available cell holders from Beckman Coulter can be configured as well as a custom cell holder with up to 12 positions Figure 92 Cell Holders A901285P Al The following cell holders are available Single Cell Holders Ambient adjustable Water
157. w accessible in the Diagnostic Menu should be examined as part of the Preventive Maintenance program System Status Window Screen Administrator Paul Novak Lab 101 Model DU 800 Instrument Name 04305 Serial Number 800 0062 Firmware Version 1 26 Visible Source Usage 752 3 hours 38 of typical life expectancy UY Source Usage 1297 1 hours 130 of typical life expectancy Windows Version 2000 Build 5 09282 Software Version 1 00 Language English Help Print Computer Info Close The window shows among other things the amount of time that both source lamps have been in use The UV source should be changed at 1000 hours The visible source should be changed at 2000 hours Fuse Replacement The only user accessible fuse is the power fuse located above the receptacle where the power cord is plugged into the instrument The instrument is shipped with the fuse installed 1 Turn the instrument OFF 2 Turn the instrument around so that the back can be accessed 3 Unplug the power cord from the instrument 4 The power line module contains a power on off switch a power cable receptacle and a fuse compartment with one fuse installed To access the fuse pull and lower the fuse compartment as shown below The fuse is specified for 100 to 240 VAC T3A 250V P N 890895 130 DU 800 Spectrophotometer Installation amp Operating Instructions Figure 98 Fuse Compartment 901281L Al
158. without a valid license it will inform the user via a message box that this an invalid license When the DU 800 System and Appplications Software has been installed on a computer which is not connected to a DU 800 Spectrophotometer software options can be installed from the floppy disk that includes the license However it is important to notice that the software option must first be added to a computer with the DU 800 Spectrophotometer connected ATTENTION Licenses for software options are provided on a floppy disk It is recommended to Create a Backup License File after one or more software options have been added Make sure to store the file in a safe location Create Backup License File The Create Backup License File function creates a backup file that includes all licenses for the currently installed software options This function is recommended and should be performed after all software options have been added ATTENTION Create a Backup License File after the software option s have been added MAKE SURE TO STORE THE BACKUP FILE IN A SAFE LOCATION 99 DU 800 Spectrophotometer Installation amp Operating Instructions In the case of an emergency or if the software is re installed on another computer the backup license file allows you to restore the licenses for the purchased software options Security Enhancements Version 2 0 Build 74 includes new features for data security 21 CFR Part 11 complianc
159. y at 1A with NIST 930D solid filter at 546 nm see RMS Noise at OA average of 10 standard deviations of lt 0 0002A RMS 10 readings at 0 05 sec intervals at 500 nm Stray Light measured using Nal at 220nm lt 0 05 per ASTM E387 84 Stability OA constant ambient conditions measured lt 0 003A hr for one hour at 340 nm after one hour warmup RMS Baseline Flatness 0 001A RMS from 200 to 900 nm at 0 A Physical amp Environmental Specifications Width 69 cm 27 inches Depth 53 cm 21 inches Height 26 cm 10 inches Weight 33 kg 73 pounds Line Voltage 100 240V 10 Frequency 50 60 Hz Power 200 watts typical Ambient Temperature Operating Range 15 to 40 C 59 to 104 F Humidity lt 85 maximum relative humidity not to exceed 32 5 C WBT These specifications apply to the optical bench which is the DU 800 Spectrophotometer without a computer DU 800 Spectrophotometer Installation amp Operating Instructions Maintenance A NOTICE Refer to the SAFETY NOTICE before following replacing any items Refer all servicing for procedures not contained in this section to qualified service personnel General Information The DU 800 UV Visible Spectrophotometer is a PC controlled optical instrument For the system to operate properly it is necessary to have adequate ventilation as described in Installation It is necessary to operate the instrume

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