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User Manual 2015 - MCEM

1. MCEM User Manual 2015 USING MCEM Enquiries regarding use of MCEM facilities can be directed to the Manager Dr Peter Miller Projects The Project is the basis for all work carried out in MCEM and it is essential that you understand this concept A Project is a body of work with a specific affiliation and a defined purpose A Project must be closed and a new Project may be created if the Project s affiliation changes or if there is a significant change in its purpose A Project may also be closed and possibly a new Project may be created to meet the administrative needs of Supervisors MCEM uses Project Numbers to track use of microscopes and other equipment and for charging The Project Number is needed when making a microscope booking and when filling in logbooks Limits on microscope use apply to the Project not to the User A Project can have multiple Users and Supervisors and a User can have multiple Projects The User s Supervisor s and Project may all have different affiliations A User cannot have more than one Project for the same purpose How To Request A New Project 1 Fill in the MCEM Project Request Form This form Word or on line PDF document can be downloaded from the MCEM web site http www mcem monash edu au Return the form by email to mcem monash edu Your form will be evaluated by the Manager The Manager can immediately create a new Project for your work if a you are a current M
2. 18 MCEM User Manual 2015 INSTRUMENTATION AND SUPPORT FACILITIES MCEM relocated into the new building Building 81 in November 2007 This dedicated building provides exceptional acoustic mechanical thermal and electromagnetic stability as well as high quality water gas and power services needed to ensure optimum instrument performance This is one of the most stable electron microscopy buildings worldwide MCEM maintains eight electron microscopes four transmission electron microscopes TEM three scanning electron microscopes SEM and a focussed ion beam field emission gun scanning electron microscope FIB FEGSEM Two atom probe field ion microscopes APFIM were removed from service at the end of 2014 Four major new instruments were commissioned in 2008 2009 including Australia s first aberration corrected transmission electron microscope the FEI Titan 80 300 field emission gun transmission electron microscope FEGTEM This is the highest resolution microscope in Australia and one of just a handful worldwide The other three instruments are the JEOL 2100F FEGTEM JEOL 7001F FEGSEM and an FEI Quanta 3D FIB FEGSEM A further four new instruments were installed during the second half of 2012 FEl Nova NanoSEM 450 FEGSEM FEI Magellan 400 FEGSEM FEI Tecnai G2 T20 TWIN TEM FEI Tecnai G2 F20 S TWIN FEGTEM Computer software and support equipment available to MCEM Users are listed in Table 3 and Table 4 Table 3 MCEM so
3. FEI Magellan 400 FEGSEM 2012 FEG 5 axis stage IR chamber camera retractable BSE detector oil free pumping system Oxford Instruments Aztec analysis system and 60 mn silicon SDD with SATW ultra thin window HKL Oxford EBSD system FEG 5 axis stage IR chamber camera oil free pumping system retractable DBS annular BSE detector low vacuum imaging LV BSE detector beam deceleration in lens detectors NavCam Helix detector plasma cleaner cryo trap Bruker Quantax 400 X ray analysis system and 60mm SDD with super light element window slew FEG 5 axis stage IR chamber camera oil free pumping system retractable DBS annular BSE detector retractable STEM 3 detector quick loader beam deceleration in lens detectors NavCam plasma cleaner cryo trap Bruker Quantax 400 X ray analysis system and 30mm SDD with super light element window slew 20 MCEM User Manual 2015 Focussed lon Beam Microscope FEG Ga FIB Pt GIS 4 quadrant BSE detector retractable STEM detector EDAX TEAM X ray analysis system with 10mmY silicon drift detector SDD and ultra thin window UTW TSL Hikari EBSD system ESEM 5 axis stage IR chamber camera Kleindiek in situ and ex situ FEI Quanta 3D FEG 2009 lift out AutoFIB AutoTEM AutoSlice and View cryo trap oil free pumping system Gatan X ray ultraMicroscope XuM with microtomography stage Removed from service 2012 200kV LaB scanning unit twin pole piece Oxfo
4. Microscope Managers work closely with Microscopists to ensure the efficient operation of the laboratory Dr Flame Burgmann one day per week SEM Manager FEl Magellan 400 FEGSEM FEl Nova NanoSEM 450 FEGSEM and JEOL 7001F FEGSEM Tel 990 56211 Office Building 81 Room G29 flame burgmannOmonash edu Dr Amelia Liu Acting SEM Manager FIB Manager FEl Quanta 3D FIB FEGSEM Tel 990 58789 Office Building 81 Room 106 amelia liuGmonash edu Dr Tim Williams TEM Manager FEI Tecnai G2 T20 TWIN TEM and FEI Tecnai G2 F20 S TWIN FEGTEM Tel 990 20721 Office Building 81 Room 103 timothy williams monash edu MCEM User Manual 2015 Microscopists Microscopists work closely with Microscope Managers to support the efficient operation of the laboratory They assist with the development of training and operating procedures and with development of new microscope capabilities and techniques Microscopists provide training and assistance to microscope Users may collaborate with Users on projects of mutual interest and carry out Client work Dr Emily Chen on leave Electron Microscopist and Specimen Preparation Specialist Tel 990 55348 Office Building 81 Room 104 yu chen monash edu Dr Xi Ya Fang Electron Microscopist and Electropolishing Manager Tel 990 20821 Office Building 81 Room 104 xi ya fang monash edu Dr Jisheng Ma Electron Microscopist Tel 990 56250 Office Building 81 Room 107 jisheng ma monas
5. N MONASH University Centre for Electron Microscopy User Manual 2015 Front view of MCEM laboratory Version 2 15 15 April 2015 MCEM User Manual 2015 Contents INTRODUCTION Moo 4 ABOUT MOEM coronan Ds eN 4 MCEM Plati ormi VISION rd EE A A E 4 vales PISO TN MISSION nissan 4 STAFF CONTACT DETAILS AND ROLES cia lada o Generales 5 PIS edet Hr 5 Manag Erea a N ode DEI aac M M M A 5 E A coctis aco Lue ENSE 5 zc er TR ES 5 Academic Star eer e E 6 Microscope Mandler CPI TS 7 MICrOSCODISTS een a TE A A E seen tans E AO M 8 NWEFOSCOPE EN SINGS S ran E E DO DDR 9 ANITE S a E eli 10 Research EMS ds 10 MCEM PhD Se olars Mp HOLE Said dal 10 Adjunct Research Felo WS dd 10 LOGA TON Mr 11 BUILDING DIRECTOR sesion 12 A uelevseaaeseneans 13 BE CENE IIO rS 17 EdDOrdtor Area Aces ds 17 After HOUS ACCESS cere c mem 18 VISIO PS ana TOUTS deals 18 INSTRUMENTATION AND SUPPORT FACILITIES eese 19 EQUIPMENT MANAGERS ENGINEERS AND TRAINERS eese nennen 22 USING MCEM P toda 24 PRO LS P 24 How To Request A New Project encino dinar SA RA AAA EO ave E eeu n rupe po pig 24 Supervisor RESP ONSID INDY acis cet emit Satna songs does uet i praedio a aaa 25 TRAINING tit rad tico 26 SEI Peal dd CP HE 26 TEMITA oca 26 SPEcimMen
6. 990 55291 Health and Safety Representative Katherine O Rourke Building Wardens Peter Miller Building Warden Russell King Deputy Building Warden Fire Wardens Assoc Prof Laure Bourgeois Dr Amelia Liu Dr Flame Burgmann Mr Renji Pan Dr Emily Chen on leave Assoc Prof Matthew Weyland Dr Xi Ya Fang Breathing Apparatus Mr Renji Pan Dr Russell King Dr Xi Ya Fang 44 MCEM User Manual 2015 First Aid Officers First Aid kits are located in Rooms G03 General Office G10 Kitchen and G35 Specimen Preparation Laboratory A The following MCEM staff members are qualified as Monash Level 2 First Aiders 4 t oY E Figure 7 Dr Tim Williams Figure 9 Dr Xi Ya Fang First aid co ordinator Building 81 Room 104 Building 81 Room 103 Tel 990 20821 Tel 990 20721 Figure 8 Dr Russell King Figure 10 Dr Amelia Liu Building 81 Room G34 Building 81 Room 106 Tel 990 53804 Tel 990 58789 45 MCEM User Manual 2015 46 Centre for Electron Microscopy MONASH University SAFETY INDUCTION CHECKLIST AND USER AGREEMENT Name Monash ID Email Telephone Department Campus Supervisor Telephone have read and understood the User Manual and agree to follow these rules and procedures at all times when using the Monash Centre for Electron Microscopy have received an introductory safety induction including instruction in the hazards and safe handling of liquid nitrogen and chem
7. External charges publicly funded researchers The charge rate for use of any microscope except the FEI Titan aberration corrected TEM by an Australian publically funded researcher is 90 hr ex GST The charge for use of the Titan TEM is provided on a case by case basis There is an additional charge of 100 150 per hour for staff time ex GST External publically funded research undertaken in collaboration with a Monash partner and intended for publication with a Monash co author may be charged at the internal rate External charges commercial entities Monash University must charge full commercial rates for work done by or on behalf of commercial entities under the Trade Practices Act administered by the Australian Competition and Consumer Commission ACCC see the Australian Consumer Law Compliance Guide http intranet monash edu au legal aclcguide html External commercial work will require costing a contract and a separate Project Number External industry Users should contact the Manager to discuss their microscopy needs Please note that negotiations for external work can be very involved and time consuming particularly when intellectual property rights are involved Undertaking external work at the internal charge rates may be both a breach of University policy and illegal under Trade Practices Act administered by the ACCC Monash Users who need to undertake microscopy on behalf of an external client must discuss this with the
8. OR Where you carried out all the work or one or more MCEM staff members are co authors The authors acknowledge use of facilities within the Monash Centre for Electron Microscopy In addition specific instruments funded by the ARC as listed in able 6 must be acknowledged as follows This research used equipment funded by Australian Research Council grant s select from table below OR This research was supported under the Australian Research Council s Centres of Excellence funding scheme COE for Design in Light Metals Table 6 MCEM instruments funded by the ARC FEI CM20 ARC Funding AL9132708 FEI Titan ARC Funding LE0454166 ARC Funding LE110100223 Publication references must be sent to Katherine O Rourke see Contact Details It would also be appreciated if a soft and or a hard copy of the publication is provided which we will place on a publication rack 33 MCEM User Manual 2015 STORAGE OF MATERIALS AND DATA Centre Users are not permitted to store materials in the building The Centre operates on a Carry in Carry out basis It is assumed that anything left behind is not needed and after a short grace period these materials will be disposed of Samples and sample preparation materials labelled with the User s name telephone number date and composition where necessary can be left in designated areas within the specimen preparation laboratories for short periods while samples ar
9. 2015 Table 5 MCEM Staff responsible for specific equipment Category Equipment Equipment Equipment Trainers Manager f Engineer SEM BEEN Xi Ya Fang Um Ma XF JM RP KW AL EEUU EN Kathryn Waldron Jisheng Ma JM KW XF AL PM Jisheng Ma 7001F Renji Pan Renji Pan RP JM XF FIB SEM Quanta 3D FEG Jisheng Ma Renji Pan JM DV XF RP AL l PM David Vowles E F20 TW RK MW Support Plasma Cleaner Matthew Weyland Russell King MW TW LB RK Equi t quipmen Gentle Mill 2 Tim Williams Russell King TW RK MW PIPS 1 and 2 Tim Williams Renji Pan RK TW MW Electropolishing Xi Ya Fang XF RP LB Tim Williams Russell King RK TW APFIM Russell King Russell King RK Electropolishing Tim Williams Russell King RK TW MW Ultramicrotomes Tim Tim Wiliams disheng Ma Ma JM RP MRPSG O0 Ultrasonic drill Williams E King TW aus MW LB dimpler hand grinder Ecomet 3000 Renji Pan Renji wm Polisher NM Techprep Polisher Tim Williams Russell King dmm MW iud MERE ou ARA A Equipment Sputter coaters coaters Renji Pan Pan Renji Pan Pan RP BE dia Tegramin 30 HER us Pan a automated polisher General SEM Renji Pan Renji Pan Specimen Prep Including saws Optical Olympus BX51 Tim Williams Tim Williams ERN microscopes optical microscope Olympus SZX16 Tim Williams Jisheng Ma uM HP TW stereo microscope Nikon Nikon Eclipse Ni Ni Renji Pan Pan RP JM 23
10. Preparation TRAINER 27 MCEM User Manual 2015 Additionab o A agar aus iucsoededsaransadiaesiads uacdo rq dos utc ar cu edu 27 RerresHer TESIBIDB oie add 27 PRES OMS ION MOS eT TT T rere 27 Trainer M scele cT T T UU m 27 Trainee s Responsibility auci detener edipi suduE om uqUe 27 UNDERGRADUATE COURSE PRACTICAL CLASSES situa eoo e ee uten eaten 28 HONOURS AND 4 YEAR PROJECTS cc cccccesscesccsesseeeeecesesesecsseeeeeecesaeeesecseneeasenseaeeeeecsenteseens 28 EQUIPMENT ERSENGES tein htutent NEA Re PIU Riv aiii 29 EVE CWC TUDO RU E 29 BOOKING INFORMATION AND RULES 5t init 30 o DOE EOS TEL ES 30 How To Make An Online Instrument Booking cccooccnccnccnonnncnnonacononccnnnncononanrononacnnnnnonnnaronanoarononannnnns 31 How To Cancel An Online Instrument BOOKkIN8 ooccccoocnncnnccnncncnnnonccnonarononannononacnnononnnnnrononanrononannnnns 31 Salate 32 ernal Charee tc arene em Un Duis eat in D cido DNE 32 External charges publicly funded researchers ii 32 External chiargesecomtmmercialentltleS oae oim Do D vto at a d a AA 32 PUBLICATIONS AND ACKNOWLEDGEMENTS eeeeeseeeeeeeeene nennen nennen nnne nnns 33 STORAGE OF MATERIALS AND DATA aii rod 34 USB MEMORYSTICK Stand 34 Data storage and iO A N A RN 34 MICROSCOPE LABORATORY DOORS ia 35 A O eee UD SUM 35 SAFETY RULES AND LABORATORY PROCEDURES 2 lance iia 36 General RUE ranas 36 After Hours Operator isis 36 Personal Protective C
11. Room 109 is for MCEM staff use only 13 MCEM User Manual 2015 Table 1 MCEM Room Directory and Telephone List Room o 302 Monash Centre for Electron 990 58774 Main entrance Microscopy MCEM staff only G03 G04 MCEM General Office Ms Katherine O Rourke 990 55563 Executive Assistant MCEM Director G06 Dr Peter Miller 990 55291 Office o pame ee mv Wwe 700 310 Tea Room 990 58781 G11 Meeting Room 990 58782 MS G15 TEM Laboratory Lobby 990 58783 Licensed users only FEI Tecnai 120 FEI Tecnai F20 G16 Utility Room Laboratory G17 MCEM staff only G17 FEI Tecnai G2 F20 S TWIN 990 51834 Licensed users only FEGTEM G18 FEI Tecnai G2 T20 TWIN TEM 990 59891 Licensed users only G19 Utility Room Laboratory G18 MCEM staff only G20 FEG TEM Operators Room 990 58784 FEG TEM licensed users FEI Titan FEG TEM ony JEOL 2100F FEG TEM G21 FEI Titan FEG TEM 990 58785 Licensed users only G21A Utility Room Laboratory G21 MCEM staff only Utility Room FEG TEMs MCEM staff only JEOL 2100F FEG TEM 990 58786 2100F licensed users only Cleaner s cupboard Cleaner only Focussed lon Beam Microscope Licensed users only Lobby Focussed lon Beam Microscope 990 58787 Licensed users only G G G G 22 23 24 25 26 G MCEM User Manual 2015 Room E opecimen Preparation 990 58788 MCEM staff and APFIM Laboratory B licensed users only G28A Utility Room Laborator
12. and Incident Report Form can be obtained from the Safety Officer or from http www monash edu au ohs forms hazard incident report pdf 40 MCEM User Manual 2015 Chemical spill kits are located in rooms G35 G37 and corridor G48 East corridor Chemical spills requiring cleanup are classified as Incidents and must be reported immediately to the Safety Officer or Manager Deputy Safety Officer Spills involving electropolishing solutions require special cleaning procedures detailed in the Safe Working Procedures for electropolishing solutions and must be handled by licensed electropolishers or members of the Breathing Apparatus BA team Report any spill immediately to the Safety Officer or Manager Deputy Safety Officer Licensed electropolishers may only clean up minor spills less than 50 ml Larger spills must be cleaned up by the Breathing Apparatus team Broken glass must be disposed of in the broken glass containers EMERGENCY EVACUATION Normal Business Hours Evacuation On hearing the ALERT tone Beep Beep or seeing the flashing orange ALERT light Open office or laboratory door and listen to instructions over the Public Address System Prepare to evacuate put equipment into a safe standby state save files secure personal items On hearing the EVACUATION tone Whoop Whoop or seeing the flashing red EVACUATION light Close but do not lock office laboratory doors Follow instructions given by Floor Ward
13. cine WV ai COINS conri TOO OT Nm TET 44 Ere Wardens A Po nn mn is ta tavensbtecew EA a 44 BRCALMING ADD AUS diese iiem PME DE ME NA IPM IM OMM IS EE 44 AO a APP o E ce emitnd ca seus poduauanncaumeaemoneuen seas 45 SAFETY INDUCTION CHECKLIST AND USER AGREEMENT eee 47 MCEM User Manual 2015 INTRODUCTION This Manual is a convenient reference giving information about the Monash Centre for Electron Microscopy MCEM its staff and facilities operating procedures and occupational health and safety matters If you have any questions or comments regarding the Manual or MCEM please contact the Manager Dr Peter Miller see contact details below ABOUT MCEM Monash University has a strong integrated network of world class technology research platforms These are made up of core facilities and capabilities that provide high quality specialist research services see http www monash edu research infrastructure platforms Our core technology research platforms are coordinated through the Office of the Pro Vice Chancellor Research and Research Infrastructure The Monash Centre for Electron Microscopy is a central university research Platform MCEM Platform Vision To be at the forefront of research in electron microscopy providing a leading edge capability that will enable researchers to solve major scientific challenges MCEM Platform Mission To enable and advance research excelle
14. commissioned in 2008 2009 including Australia s first aberration corrected transmission electron microscope the FEI Titan 80 300 field emission gun transmission electron microscope FEGTEM This is the highest resolution microscope in Australia and one of just a handful worldwide The other four instruments are the JEOL 2100F FEGTEM JEOL 7001F FEGSEM and an FEI Quanta 3D FIB FEG SEM A further four new instruments were installed in 2012 FEl Nova NanoSEM 450 FEGSEM FEI Magellan 400 FEGSEM FEI Tecnai G2 T20 TWIN TEM and FEI Tecnai G2 F20 S TWIN FEGTEM To learn more about MCEM please visit our website www mcem monash edu au MCEM User Manual 2015 STAFF CONTACT DETAILS AND ROLES General Office Ms Katherine O Rourke Executive Assistant Tel 990 55563 FAX 990 53600 Office Building 81 Room G03 G04 katherine orourke monash edu Director Professor Joanne Etheridge Director Tel 990 51836 Office Building 81 Room G05 joanne etheridge monash edu Manager Dr Peter Miller Manager Tel 990 55291 Mobile 0418 123 584 Office Building 81 Room G06 peter miller amp monash edu MCEM Web Page http www mcem monash edu Email mcem monash edu MCEM User Manual 2015 Academic Staff Academic Staff members conduct their own research programmes usually in the development of advanced methods of electron microscopy and their application to the study of materials They may collaborate with Users on projects of mutua
15. plate reader Gatan 601 ultrasonic disc cutter 21 MCEM User Manual 2015 Gatan 656 dimple grinder two units Gatan 682 Precision Etching and Coating System PECS and Perpendicular Slope Cutting Tool Gatan 691 Precision lon Polishing System PIPS two systems Gatan 950 Solarus advanced plasma system Leica EM UMC cryo ultramicrotome Leica M165C stereo optical microscope with digital camera LKB KnifeMaker Type 7801A Nikon Eclipse N optical microscope Olympus BX51 stereo optical microscope with digital camera Olympus SZX16 stereo optical microscope with digital camera Reichert Ultracut S and FCS cryo ultramicrotome Struers Accutom 50 precision saw Struers Citovac vacuum impregnation unit Struers Lavamin specimen cleaning unit Struers Lectropol 5 electrolytic polisher Struers Tegramin 30 automated polisher Struers Tenupol 2 3 and 5 twin jet electrolytic polishers Technoorg Linda Ltd Co Gentle Mill 2 EQUIPMENT MANAGERS ENGINEERS AND TRAINERS Equipment within MCEM is supported by Equipment Managers Engineers and Trainers see Table 5 with the following responsibilities The Equipment Manager is the staff member who is most familiar with the detailed operation of the equipment The Equipment Engineer is the staff member with primary responsibility for the day to day operation and routine maintenance of the equipment An Equipment Trainer provides training in use of the equipment 22 MCEM User Manual
16. session to refresh your memory of the instrument s operation and to bring you up to date with any recent changes in operating procedures Unless otherwise stated licences permit use of microscopes between 9 00am and 5 00pm on normal University business days A list of authorized personnel is displayed outside each laboratory in the Centre Only authorized personnel may enter a room unsupervised Experienced Business Hours operators can apply to the Microscope Manager for an After Hours licence Licence types Waiting You are in the queue for training If you expect to trained on a particular microscope you should check that you do have a Waiting Licence If not please contact the microscope manager Client A Centre staff member will operate the microscope for you and unless otherwise arranged you will need to be present to identify the features of interest in your samples Training Under the direct supervision of a Trainer Supervised Use Under the supervision of a Trainer Trainer will exchange samples exchange the camera TEM start up and shut down the microscope but you will record your data 9 00am to 5 00pm Business Hours Unsupervised use during business hours 9 00am to 5 00pm After Hours Unsupervised use at any time that the University is open Trainer After Hours licence plus authorised to train Users Manager Trainer licence plus can grant a new licence 29 MCEM User Manual 2015 BOOKING INFORMATION AN
17. to complete and sign the Safety Induction Checklist And User Agreement on the last page of the Manual Note The User is the person who has the microscope licence The Supervisor is responsible for authorising the work and for payment of any costs incurred such as for training consumables equipment use or the cost of repairing equipment damaged as a result of incorrect operation by the User The User and Supervisor can be the same person Supervisor s Responsibility The Supervisor of a Project is responsible for authorising the work and for payment of any costs incurred such as for training consumables equipment use or the cost of repairing equipment damaged as a result of incorrect operation by a User on this Project 25 MCEM User Manual 2015 TRAINING Your training needs will have been identified during your initial interview MCEM instruments can only be used by trained and licensed Users A range of licence types are available depending on your skills and needs see below Training sessions will use standard samples and are not intended to yield scientific results Electron microscopy encompasses a number of difficult and complex techniques each of which requires sustained and dedicated learning to master Depending on the technique this can take months or years After you have completed your initial training you must then make regular use of the microscope over a period of some months to reinforce the training Your traini
18. CEM User b your new Project involves similar microscopy to be carried out on similar materials to your previous Project s c the work is authorised by your supervisor where applicable and d you don t need any additional microscopy or specimen preparation training 4 f any of the above do not apply you will be contacted to arrange an interview to discuss your new Project This interview will be attended by yourself your supervisor compulsory for students desirable for junior research staff relevant MCEM staff members Microscope Managers and specimen preparation specialists and the Manager The new Project interview will determine the following e The research problem to be solved e If MCEM facilities are suitable for solving this research problem e fso which microscopes and specimen preparation techniques are needed e he scope of the work MCEM facilities are in great demand and there are limits on the amount of microscope time that you can use e What microscope and specimen preparation training is required e Safety issues 24 MCEM User Manual 2015 It is required that you have samples available at the time of the new Project interview Users are normally trained to do their own microscopy unless the amount of work involved is relatively small or if the microscopy and interpretation is too complex in which cases it is more efficient for MCEM staff to perform the microscopy This is called a Client Project
19. D RULES Microscopes can be booked through the MCEM web page see www mcem monash edu au embookings You must be a licensed User to access the booking system Users with a Client licence Training licence or Supervised Use licence can check on bookings but cannot change them Instrument manuals training materials and help information is also available on the Bookings page Bookings can only be made a limited time in advance typically two weeks and there will be a limit on the number of sessions or hours that can be booked within this period These limits apply to the Project not to the User Booking limits will vary from time to time depending on instrument load Please contact the Instrument Manager if extra sessions are required urgently Activities such as repairs and maintenance undergraduate classes and training of new users are usually undertaken during normal business hours and occasionally this will significantly limit the time available for regular bookings Please note that you are charged for the greater of either the booked time or the logged time From time to time you must check My Details to ensure that your user details are correct In particular it is essential that your email address is correct If email sent to you bounces then it will be assumed that you have left Monash University and your licences projects and access will be cancelled While checking your details on the My Details page you may wish to elec
20. Manager well in advance of when they plan to start the work 32 MCEM User Manual 2015 PUBLICATIONS AND ACKNOWLEDGEMENTS Your use of Centre facilities is heavily subsidised by the University and by grants from external funding agencies In order to comply with current funding obligations and to apply for new funding the Centre must report on the number and type of publications produced by Users of the Centre It is essential that you the User provide us with this information so that we can continue to provide the advanced instrumentation staff training and assistance that you and other researchers require to undertake your research work It is a condition of use of the Centre that 1 You provide the reference for all publications and reports that contain results obtained using Centre facilities including instrumentation computer software and staff advice 2 You acknowledge the Centre and any assistance provided by Centre staff in the publication or report 3 Centre staff members who have acquired and or interpreted data on your behalf should be invited to be co authors on the publication as is usual practice Please acknowledge the contributions of the Centre in your publications simply by including Where Centre staff provided assistance but not enough to justify co authorship The authors acknowledge use of the facilities and the assistance of NAME OF MCEM STAFF MEMBER S at the Monash Centre for Electron Microscopy
21. The User is expected to be present while their samples are being examined and work by MCEM staff must be appropriately acknowledged Please note that due to the heavy demand for access to MCEM facilities and for training it is very difficult for MCEM to accommodate urgent requests It may be a month or more before training can commence In the mean time if you have urgent microscopy needs MCEM staff can undertake a limited amount of microscopy on your behalf Please also note that you will only be trained if you can make regular use of the microscope over at least the next six months following your training This is essential to consolidate you skills in operating the microscope following training As part of your training you must use the microscope at least twice within a few weeks of being granted a licence Your licence will lapse if you don t do this The Microscope Manager s will contact you to arrange training as determined at the User interview You must complete an Induction before you can work unsupervised in the building You will be informed of the next available Induction session Prior to attending the Induction you must download and read the MCEM User Manual this document The Manual PDF file can be downloaded from the MCEM web site http www mcem monash edu au or a printed copy can be obtained from the General Office You must bring this Manual with you to the Induction session After completing the Induction you need
22. ash staff and students Users with an After Hours licence have 24 hour access to the relevant laboratories on normal University working days via the main entrance and the door from Reception into the Laboratory Area All people entering the building must fill in the After Hours Logbook in Reception and must ring Security 53059 on arrival and departure with the following exceptions 1 between the hours of 7 00am to 8 00pm on normal University working days 2 supervised official MCEM activities such as Open Days seminars and conducted tours Visitors and Tours Visitors to the main Laboratory Area must sign in and out at Reception must wear a Visitor Badge and must be under the supervision of a MCEM staff member or an authorised User at all times MCEM has frequent requests for tours Wherever possible it endeavours to accommodate these requests however tours needs to be conducted in a way that has minimum impact on MCEM users If you would like to arrange a tour of MCEM please send a request to the Manager preferably at least one week in advance Please note that microscopes are heavily booked and that microscope doors are kept closed Microscope operators have limited time to get their work done and cannot be interrupted It is possible that the tour will not be able to enter any microscope room The earlier that MCEM is notified of a tour the greater the chance that the tour will be able to include a visit to the major instruments
23. atory Procedures comprise part of the MCEM Safety Induction that all Users are required to complete Note that these are the general rules and procedures applying to all people using the Centre You will be instructed in any additional rules and procedures that apply for specific items of equipment as part of your training For information regarding OHS Risk management at Monash University see the Monash OHS Web Page http www monash edu au ohs index html Where any hazard was identified with either your samples or the sample preparation methods to be used a Risk Control Worksheet RCW must be prepared by the User and signed by the User Supervisor and Instrument Manager see Table 5 If a hazard has been assessed and after taking into account all normal methods of risk minimization the risk is still medium or high then a Safe Work Instruction SWI must be prepared by the User if a suitable SWI has not already been prepared Note an SWI may also be called a Safe Working Procedure All RCWs and SWls with risks that remain as medium or high must be checked and counter signed by the Safety Officer or Deputy Safety Officer Materials Safety Data Sheets MSDS are downloadable using Chemwatch General Rules If in doubt always seek help You must comply with any temporary notices or tags that may be used from time to time in the Centre For example Do Not Operate notices and warnings of changed operating conditions due to equipme
24. dle liquid nitrogen As liquid nitrogen gasifies there is a large volume increase never store liquid nitrogen in sealed vessels Liquid nitrogen must not be removed from the Centre Be aware that liquid nitrogen will cause liquid oxygen to form on cold surfaces by condensation from the atmosphere Compressed Gases The following compressed gases are used in the Centre e Nitrogen e Argon e Sulphur hexafluoride e Helium e Neon e Oxygen e Hydrogen e Argon Oxygen e Natural Gas All microscope rooms and most laboratories have reticulated nitrogen supplies There is an asphyxiation hazard if there is a major nitrogen leak and the air conditioning is not operating for example during a power failure YOU MUST NOT ENTER OR REMAIN IN A LABORATORY WHILE THE AIRCONDITIONING IS INOPERATIVE Note Gas isolation taps are located outside the gas store There is an alarm panel above the gas isolation taps This alarm warns of low gas pressure indicating that a gas bottle needs changing or low nitrogen pressure this is not a hazard alarm Keep gas cylinders secured in their racks Do not attempt to move or perform any maintenance on gas cylinders regulators or other gas fittings Hazards And Incidents Chemical Spills Breakages Any occurrence that leads to or potentially leads to injury or danger to health must be reported to the Safety Officer Dr Russell King and the Manager Deputy Safety Officer Dr Peter Miller The Hazard
25. e actually being made The Centre can assist you if you need to store your samples under special conditions for example vacuum or inert gas TEM negatives must be removed from the dark room once dry and must not be left on light boxes USB MEMORY STICKS Use of USB memory sticks or similar is NOT permitted on MCEM computers as these are a source of virus infection A number of computers are not protected by virus checkers as the manufacturer will not guarantee equipment performance if any additional software is installed Computers without virus checkers are not connected to the network Data storage and transfer Storage and security of your data is your responsibility Data can be lost at any time due to hardware failure software upgrade or fault or user error In general you should not leave your data on MCEM computers However in some circumstances where you need access to your data during subsequent microscope sessions files can be left on an MCEM computer Each microscope computer and computer room computer has a local data folder in which you may create a sub folder for your temporary use The sub folder name must be your first name or initial and surname followed by your Project Number so that we can identify the owner of the data Folders that do not conform to this format or files created anywhere else including the desktop will be deleted Please delete all of your data from MCEM computers when it is no longer needed A ne
26. ed immediately dial 000 from your mobile telephone or dial 0000 from a university telephone with access to an outside line Foyer and Specimen preparation Laboratory G35 The decision of calling emergency services is at the discretion of the person confronted with the situation State e Nature of Emergency e Building Number 81 e Nearest room number e Your name Once the required emergency service has been notified Monash Security must be contacted on 333 for notification in order that the emergency services can be escorted to the scene by security staff in a timely manner Further information can be obtained from the Emergency Procedures booklets that have detailed information on emergency response procedures including injury protocols You should make yourself aware of the processes in these booklets which are next to every internal phone The Monash Security website http www fsd monash edu au security also is an excellent source of information For less urgent matters you can ring the Gate House on 53059 Fire Extinguisher Locations Room G38 Gas Store Room G10 Tea Room Corridor outside Room G20 FEG TEM Operators Room Corridor opposite Room G38 Gas Store Corridor outside Room G43 FEI Nova SEM Room Fire system cupboard in Room G02 Entrance foyer Inside Fire Hose Reel cupboard next to mezzanine tea point Room 110 Research Fellows Office Emergency Eye Wash And Showers There are Eye Wash Stations and Em
27. ens Yellow Hats Leave the building by the nearest safe exit Proceed to the ASSEMBLY AREA For Building 81 this is the grassed area to the East of Building 75 and adjacent to Building 53 see Figure 4 Remain at the ASSEMBLY AREA until a Warden or their delegate gives the ALL CLEAR After Hours Evacuation Evacuate on either of the ALERT or EVACUATION warnings Proceed to ASSEMBLY AREA until ALL CLEAR is given see Figure 4 Power Failure Microscopes are powered by Uninterruptible Power Supplies UPS backed up by a generator and will continue to operate in the event of a power failure However all other services such as general power outlets lighting and air conditioning will fail Emergency lighting and torches are provided in microscope rooms In the event of a power failure lasting more than a minute or so you should put the equipment into a safe standby state see microscope Operating Procedures and leave the laboratory area You can remain in the office or administrative areas until the situation is resolved If the power failure lasts more than 30 minutes then you must not re enter the laboratory area until a Centre staff member advises you that it is safe to do so YOU MUST NOT ENTER OR REMAIN IN A LABORATORY WHILE THE AIRCONDITIONING IS INOPERATIVE see Compressed Gases 41 MCEM User Manual 2015 IN AN EMERGENCY Contacting emergency services If emergency services Ambulance Police Fire Brigade need to be contact
28. ergency Showers in Rooms G27 G35 and G37 42 MCEM User Manual 2015 Emergency Evacuation Plan JECIMNMENECES queja aul Joyeuqyag dav pued xxexpur any 31 asino F moros nue E pa asoy au G as a BR MEE JURIpAy aut4 urod 123 enut ES ACME 106 WEW ES QN39341 IO8WAS eae quiasse o ajenoe 3 doou doouM wasis yd SU Je o sjueureounouue JO uajsi 9 juswdinbe yo wy ayenoena o eJedaug daag daag SIN IV TIV vosjad pasuoyne ayy Aq uaAIB si Jesja e au pun quay Aeys y Bue quiasse eu o SJENIEAZ ui E asn JOU OG unu you og HIEM Buipying ayy BunenaeAe uayM suueje ayy Buueaq ug seowes ouePueue JO suapueM yejs Aq pajongsul y e sugea Jabuep e Buipjinq ay sea 03 peas ag NOILVn2VA3 OS Op 0 SJES S Y JI diay speau OUM SUO UVE ISISSY e ig euoudejal Aquahiawa pay e asnio e 65066 O 0 cct xo fal junoeg peo SAIDNADYSAWSA TIV HO E uue e ssej yea1q JENUEW pay e aJenpoy 34134 403 SEEDnRERBPSEEBEGIEBEEITE JeldH dV NOA 9197 dossoJ uoJ 99 3 10J 9Jju92 useuoJA AE Ie NOILVNOVAA bwpdg 56oreno0 S E Z ZO ELOZ VIYW A 18l43SSV OL v 18 USISAIUN HSVNOW amp Figure 4 Emergency evacuation plan 43 MCEM User Manual 2015 Safety Officers Figure 5 Safety Officer Figure 6 Deputy Safety Officer Dr Russell King Dr Peter Miller Building 81 Room G34 Building 81 Room G06 Tel 990 53804 Tel
29. f lab coats safety glasses and gloves There are a number of lab coats and safety glasses provided at both entry points for your convenience Please do not bring external lab coats from your department into this lab unless by prior arrangement Laboratory Use No food or drink may be stored or ingested in any laboratory Equipment logbooks must be filled in at the start and end of a session Note any unusual conditions or equipment problems and report these to a Centre staff member Always follow the Operating Procedures provided with each piece of equipment Access to the electropolishing room is restricted by proximity card access and is strictly limited to licensed Users of electropolishing equipment and trainees under direct supervision Never attempt to repair any equipment Any problems faults or unusual behaviour must be reported in sufficient detail in the logbook and to a Centre staff member Names of Centre staff members responsible for laboratories can be found on the Laboratory Protocol sheets Names of Centre staff members responsible for equipment can be found in Operating Procedures and in Table 5 No items of equipment tools or consumables can be removed from the room in which they belong without the permission of a Centre staff member Clear and clean your work area when finished Glassware must be cleaned by the User immediately after use and must not be removed from the Centre Samples and sample preparation material
30. ftware and A ofa pg Semester platform ___ platform n v5 several copies Bruker Esprit EDS X ray analysis software on microscopes DA A and multiple off line copies microscope and off line EDAX TEAM Gatan Digital Micrograph V1 and V2 E microscopes and PC UU RR off line copies HOBOwarePro i s 7 Pro Image and diffraction pattern simulation programs based on BA Multislice and Bloch wave methods in house ImageJ eee J JEMS gae e copies Matlab eee production Casino Win Xray and in ET and multiple off line OE HKL Channel 5 EBSD Suite on microscope and off line PDF4 2009 ICDD Crystal Database SpectrumPlot OP SpectrumPlot 19 MCEM User Manual 2015 Table 4 MCEM equipment list Transmission Electron Microscopes Double corrected FEI Titan 80 300 FEGTEM 2008 JEOL JEM 2100F FEGTEM 2008 FE Tecnai G2 F20 S TWIN FEGTEM 2012 FEI Tecnai G2 T20 TWIN TEM 2012 300kV aligned for operation at 80kV 200kV and 300kV FEG TEM STEM Super Twin pole piece CEOS Cs probe corrector CEOS Cs image corrector Fischione Instruments 3000 ADF detector on axis BF DF detector Bruker Quantax 400 STEM X ray analysis system with windowless 60mm SDD TIA software Gatan Tridiem 863 P image filter Gatan UltraScan 1000 P 2kx2k CCD camera tomography and high stability tomography goniometer Gatan 636 double tilt cooling holder HCHTR3000 d
31. h edu Dr Kathryn Waldron part time Electron Microscopist Tel 990 53435 Office Building 81 Room 107 kathryn waldron monash edu MCEM User Manual 2015 Microscope Engineers Microscope Engineers play a pivotal role in the day to day running of the laboratories They undertake maintenance on specific instrumentation and associated infrastructure see Table 5 and may be involved in the further development of these instruments to optimise and extend performance Microscope Engineers provide training and assistance to microscope Users see Table 5 and may carry out Client work They are usually your first point of contact when a problem is encountered with an instrument Mr Selwyn Glanvill by arrangement Ultramicrotomist Tel 990 20822 Office Building 81 Room G30 selwyn glanvill monash edu Dr Russell King Microscope Engineer and Safety Officer Tel 990 53804 Office Building 81 Room G34 russell king monash edu Mr Renji Pan Microscope Engineer Tel 990 54931 Office Building 81 Room G34 renji panQmonash edu David Vowles visiting Microscope Engineer Tel 990 53435 Office Building 81 Room 107 david vowlesQmonash edu MCEM User Manual 2015 Affiliates Affiliates typically hold a joint appointment with MCEM and a School or Department They conduct their own programmes of research usually in the development of methods of microscopy and their application to the study of materials They may be avai
32. icals in the Centre and on appropriate personal protective equipment Detailed instruction as required will be provided during training have been advised of emergency procedures including location of fire extinguishers evacuation procedures and the emergency assembly area have been advised of first aid provisions including locations of First Aid kits how to contact a first aider the location of safety showers eyewash stations and spill kits have been introduced to the Safety Officer Where necessary have performed a Risk Assessment on my work and have informed the Safety Officer and Instrument Manager of any associated hazards have been advised of need to obtain a licence to use equipment and laboratories have been advised that only authorized personnel Licensed Users may enter a laboratory unsupervised have been advised of the location of Risk Assessment Risk Control Worksheets Safe Working Instructions for all laboratory activities equipment and processes have been advised how to obtain MSDS information for chemicals used within the Centre have been advised of After hours procedures Signature of User Date Signature of MCEM Manager Date 47 Yes LJ Yes LJ Yes L Yes LJ Yes LJ Yes LJ Yes LJ Yes L Yes L Yes L Yes L 48
33. l interest requiring advanced electron microscopy Academic Staff are responsible for leading the development of new methods and instrumentation to maintain and extend the MCEM s advanced capabilities Academic Staff members also perform the role of Microscope Manager see below Professor Joanne Etheridge Director Tel 990 51836 Office Building 81 Room G05 joanne etheridge monash edu Associate Professor Laure Bourgeois Associate Professor and JEOL 2100F FEG TEM Manager Tel 990 55368 Office Building 81 Room 105 laure bourgeois monash edu Associate Professor Matthew Weyland Associate Professor and FEI Titan FEG TEM Manager Tel 990 59026 Office Building 81 Room 108 matthew weyland monash edu MCEM User Manual 2015 Microscope Managers The Microscope Manager is responsible for overall management of a microscope its performance and maintenance preparation of operating manuals and training materials management of training client work internal and external and issuing licences setting and enforcing training procedures operating procedures access rules and booking regimes instrument and technique development provision of expert advice to assist with equipment purchase The Microscope Manager provides training advice and assistance to Users particularly where advanced techniques are needed The Microscope Manager may collaborate with Users on projects of mutual interest and may carry out Client work
34. lable to collaborate with Users on projects of mutual interest requiring advanced microscopy They are not routinely involved in microscope management training or service Research Fellows Dr Hadas Katz Boon Dept of Materials Engineering Dr Michael Walsh Dept of Materials Engineering Dr Changlin Zheng MCEM Research Fellow Dr Ye Zhu Dept of Materials Engineering MCEM PhD Scholarship Holders Mr Yueming Gao Dept of Materials Engineering Mr Dan Nguyen Dept of Materials Engineering Adjunct Research Fellows Professor Alex Moodie FAA Dept of Materials Engineering Dr Chris Rossouw Dept of Materials Engineering Professor Harry Whitfield Dept of Materials Engineering 10 MCEM User Manual 2015 LOCATION The Monash Centre for Electron Microscopy is located at 10 Innovation Walk on the Monash University Clayton campus see Figure 1 The main entrance is at the North West corner For instructions on travelling to the Clayton campus see http www monash edu au campuses clayton html or Melways maps 70 and 875 Information on Parking at Monash can be found at the following link http www monash edu people transport parking permits Key Foe aa Lin part dere Bd a ses la ario age Fi a CAN Renna Fidi Ela om lor Motorcpcia Cappi T Taa Ni Peral SS Teis BE S B x riage fal IT de aa 15 Baur BPRA Auai cm Bice dara Losing reas WELLINGTON ROAD Figure 1 Location of the Monash Centre for Electron Micro
35. lothing And EQuipMent cccccsscccsssecccsseccceseccescecauececeeceeeeessencessegecensneeenens 37 LEO E MU Cates oe telat ate act T DT ERE 37 FLU Gal arm CUPOS ai 38 Samples AMG CWEIMNCAIS atasca 38 Engineered Nanoparticles TENPS ia iia 38 PIANOS EN PS accio 39 Emerpency STOP DUEEOFIS spider dio 39 JRS DOWO Edad 39 SFesensors and al UNS ogists a a 39 Oxysen sensors and alal MS rca desee E E ASON di 39 EUa NICPOBGL eis tvi oro oboe meta b biu Uri dum uM tu Dd LM I EDU 39 MCEM User Manual 2015 Compressed GAS CS soon leise ds essi yas xd mm Np mPsUMPII oM Uc M deu MEDIE ME Do EE E 40 Hazards And Incidents Chemical Spills Breakages cccccccccncncnnnnnnnnnnnnanananacononanonananononono nono nonanananinans 40 EMERGENCY EVAGUA TON sutil a oca ne eR ote bacio toad Dun tu ges tee nut 41 Normal Business Hours Evacuation sodes aco verbu p EFE uS debe Neb pup Uia ero Ue E bebe Du Ede UP de bI o tei pP EUpdu E SE EDS 41 After Hour EVACUATION a MR 41 Power Falur Good ed Deo MINE EMEN IM al D MUN RN Me EC 41 INAN m maitimtad et hatte 42 Contacting emergency ServICes ese cote veis evo xe equ oe e EY ene eor eec ern o vae e equveu ei 42 Fire ExtingdiSher Locatlolnis cuidate ua Eve E ere D UE REC N 42 Emergency Eve Wash ABO SDOWOLFS ataco 42 Emergency EVacuation PlaMa csi td 43 Sale OTEO TS E REO PEC RU ete cu M MU ea Ee ML E NS RE DE 44 Health and Safety Representative is dd 44 BUIN
36. n Several deaths have been reported worldwide from exploding solutions Only licensed electropolishers are permitted to handle the electropolishing solutions and washing methanol that are stored in G37 Access to the electropolishing room G37 is by Proximity Card and is strictly restricted to licensed electropolishers and trainees under direct supervision Engineered Nanoparticles ENPs There is very little information on the safety of ENPs but as some of them will be dangerous to health the best practice is to treat all ENPs as toxic substances unless a specific nanoparticle MSDS is available 38 MCEM User Manual 2015 The most dangerous ENPs are likely to be those that are not soluble in water They cannot be eliminated from the body and therefore have a very long biological persistence Carbon nanoparticles and most metal oxides fall into this category Studies of carbon nanotubes have shown that some of them have dimensions that are similar to asbestos fibres and produce similar effects under experimental conditions Materials that are not hazardous on the micron scale may prove to be hazardous as nanoparticles due to their novel properties Gold is an example of an ENP with novel properties Although gold in its bulk form is unreactive gold nanoparticles in the 2 5 nm size range are highly chemically reactive MSDS sheets for the bulk material may therefore be unreliable hence the need for specific MSDS that relate to the nanopartic
37. nce A microscope can only be used by the person who booked it and for work identified by the Project Number Bookings are NOT transferable You must finish your session on time so that the next user is not inconvenienced 30 MCEM User Manual 2015 If instrument time is available you can extend your microscope session outside the booked time subject to your licence This actual time used must be recorded in the instrument log book How To Make An Online Instrument Booking Log in to the booking webpage at www mcem monash edu au embookings lf you have forgotten your password select Forgotten your password and follow the prompts A new password will be automatically emailed to you After login click on the instrument that you wish to book This will take you to the current week s table of bookings for that instrument Click on the desired day date of your booking From the day bookings table click on the start time of your booking left hand column then select the number of hours for your booking You must also select your Project Number from the drop down list most Users will only have one Project Number You may also enter a short notation in the Other Info text box Note If you have a Training or Business Hours licence you will only be able to book an instrument during normal hours Monday to Friday 9 00am to 5 00pm on normal University business days To complete the booking press the Confirm Booking b
38. nce at Monash University and beyond through the provision of a world microscopy for the determination of the structure of matter down to the atomic scale MCEM achieves this via its dual academic and research support role namely 1 execution of world class research in the field of electron microscopy and 2 provision of advanced instrumentation expertise and training in electron microscopy to researchers across all fields of science and engineering except biology MCEM is the largest electron microscope facility in Victoria serving several hundred registered researchers from Monash other universities government research agencies as well as providing consultancy services to industry lt plays an important role in educating supporting students in electron microscopy and materials characterisation and is a key complementary facility to the Australian Synchrotron and the Melbourne Centre for Nanofabrication MCN the Australian National Fabrication Facility s ANFF central node in Victoria MCEM works closely with Monash Micro Imaging MMI and The Clive and Vera Ramaciotti Centre for Structural Cryo Electron Microscopy which provide optical and electron microscopy facilities for the biological sciences MCEM maintains eight microscopes four transmission electron microscopes TEM three scanning electron microscopes SEM and a focussed ion beam field emission gun scanning electron microscope FIB FEGSEM Five major new instruments were
39. ng will not commence until you are able to commit the time necessary to reach a basic level of proficiency with the microscope and you have samples ready for examination By completing training to business hours level you will attain a standard of skill necessary to undertake basic microscope operations safely and effectively during normal business hours when assistance is available from MCEM staff After you have been using the microscope for some time typically at least six months you may request training for an After Hours licence SEM Training The scanning electron microscope SEM basic training course comprises both theory and hands on training beginning with a 15 minute open book test based on the instrument s user guide carried out using the Monash MOODLE online system The first day of training begins with 6 hours of lectures on SEM theory sample preparation and X ray generation This is followed by 2 x 3 hour hands on sessions working in groups of 2 covering basic operation through to optical system alignment Training continues with 1 hour of one on one training reinforcing the previous work including secondary electron SE imaging back scattered electron BSE imaging and X ray analysis The 1 hour practical test follows in addition to a 30 minute closed book written test based on the study notes and previous lecture After successfully completing the practical and theoretical test a final 1 hour session is booked with the t
40. nt faults Only operate equipment for which you hold a valid licence Doors and emergency exits must be kept clear Do not tamper with fire extinguishers first aid kits and other emergency equipment Children are not permitted in any laboratory except under supervision on Open Day No running in the building The Centre is equipped with very sensitive smoke and fire detectors Any work that may generate smoke or dust must be carried out in a fume cupboard As with all University buildings the Centre is a NO SMOKING area The wooden deck outside the centre is also a NO SMOKING area A list of authorized personnel is displayed outside the computer room and each laboratory and utility room in the Centre Only authorized personnel may enter a room unsupervised After Hours Operation Centre business hours are 8 45am to 5 30pm Monday to Friday on normal University working days All people entering the building must fill in the After Hours Logbook in Reception and must contact Security 53059 on arrival and departure with the following exceptions Between the hours of 7 00am to 8 00pm on normal University working days Supervised official Centre activities such as Open Days seminars and conducted tours After hours usage of equipment requires an After Hours licence To apply for an After Hours licence contact the relevant Instrument Manager see Table 5 36 MCEM User Manual 2015 Tenupol Electropolishing First Stage Atom Probe Elec
41. o commencement of undergraduate course practical classes in order to guarantee availability of equipment and staff Please contact the Manager to discuss your needs HONOURS AND 4 YEAR PROJECTS Supervisors proposing an Honours or 4 year project that requires use of MCEM facilities must discuss the feasibility of the project with the Manager before the project is offered to a student There is not sufficient time during an Honours or 4 year project to train the student so the microscopy will need to be done by an MCEM staff member as a Client job or by another MCEM User Please note that MCEM staff members have limited time available to carry out Client work 28 MCEM User Manual 2015 EQUIPMENT LICENCES MCEM facilities can only be operated by trained and licensed Users A licence is also needed to make a booking See Booking Rules There are a number of different licence types depending on the needs and skills of the User see below All licences will expire at the end of each year and a renewal reminder email will be sent to all Users late in the year Licences can be easily renewed by clicking a button in the Booking system You must regularly use a microscope to maintain your skills If you do not use a microscope for a period of time typically around 3 months your licence will lapse in the booking system the licence colour will change from black to red If this happens please contact the Microscope Manager to arrange a training
42. orm you of any recent changes in operating procedures Once the refresher training has been completed your licence will be restored and you will be able to make bookings as normal Responsibilities In order for microscope training to be as effective as possible the following outlines the responsibilities of the Trainer and the Trainee Trainer s Responsibilities To teach the Trainee how to operate the microscope safely effectively and without damage To instruct the Trainee in the basic principles of operation of the microscope and to teach the Trainee how to perform basic adjustment of the microscope and accessories in order to achieve reasonable performance To teach the basic microscope techniques relevant to the Trainee To provide advice to the Trainee on optimisation of the microscope s performance Trainee s Responsibilities To comply with MCEM and University OHS policies and procedures To always seek help if you are unsure of the correct operating procedure or if the equipment is not working properly 27 MCEM User Manual 2015 To always follow the Trainer s instructions on the operation of the microscope To take detailed notes To learn the theory of microscopy and related techniques by consulting books and the scientific literature Centre staff can suggest suitable references To become proficient in operating the microscope UNDERGRADUATE COURSE PRACTICAL CLASSES At least 3 month s notice is needed prior t
43. ouble tilt He holder Fischione Instruments 2020 ultra high tilt tomography holder Fischione Instruments 2040 dual axis advanced tomography holder Fischione Instruments 2050 on axis rotation tomography holder Protochips Aduro 300 double tilt heating holder to 1200 C CCEM holder dry pumping station 200 kV FEG TEM STEM high resolution pole piece piezo stage oil free pumping system Gatan UltraScan 1000 2kx2k CCD camera tomography Gatan 776 Enfina 1000 parallel detection EELS spectrometer Gatan 777 STEM Pack Gatan 692 retractable TV camera JEOL 50 mm Si Li X ray detector Gatan 901 hot stage to 1000 C Gatan 636 double tilt liquid nitrogen cooling holder JEOL tomography software Fischione Instruments 2030 ultra narrow gap advanced tomography holder Gatan 655 turbo pumping station Gatan Digital Streaming Video Software 200kV FEG TEM STEM S TWIN pole piece Gatan SC200D Orius CCD camera 2kx2k and Gatan 894 UltraScan 1000 CCD camera 2kx2k low background double tilt holder Bruker Quantax 400 STEM X ray analysis system with windowless 30mm SDD 200kV LaB TWIN pole piece Bruker Quantax 200 TEM X ray analysis system and 30mm SDD with super light element window slew Gatan 831 SC600 Orius CCD camera 2 7kx2 7k and Gatan SC200D Orius CCD camera 2kx2k low background double tilt holder multi specimen holder Scanning Electron Microscopes JEOL JSM 7001F FEGSEM 2008 FEI Nova NanoSEM 450 FEGSEM 2012
44. rainer to observe the user s sample and determine optimal operating conditions TEM Training All new TEM users start with Tecnai T20 TEM training to a Supervised User level preceded by a 15 minute open book test based on the Tecnai 120 TEM user s guide TEM supervised user training begins with two two hour lectures introducing the TEM generally held on a Monday The hands on training occupies one full day during the same week and incorporates both a written and practical test Some background reading is also required for the supervised user training and licence Once licenced Supervised users can request further training to business hours level independent operation Business hours TEM training usually requires five two hour sessions Both the initial 15 minute and the final written tests are carried out using the Monash MOODLE online system 26 MCEM User Manual 2015 Experienced TEM users needing the advanced capabilities available on the JEOL 2100F FEGTEM or Titan FEGTEM can contact the relevant microscope manager to discuss their microscopy needs Specimen Preparation Training MCEM has a wide range of equipment used to prepare samples for scanning electron microscopy and transmission electron microscopy The specimen preparation techniques that you need will have been identified during your initial interview Specimen preparation for microscopy can be a very complex and time consuming process The number of training session
45. rd Instruments Inca X Philips CM20 TEM ray analysis systems and 30mm Si Li X ray detector with SATW 1992 window Gatan 831 SC600 Orius M CCD camera 2 7kx2 7k and Gatan 805 BF DF STEM system 200kV LaB high resolution objective lens pole piece Oxford Instruments Inca X ray analysis system and 30mm Si Li X ray detector JEOL JEM 2011 with SATW window Gatan 622 SC TV camera Gatan 894 UltraScan TEM 2000 1000 CCD camera 2kx2k Gatan camera Gatan 901 hot Stage to 1000 C Gatan 636 double tilt liquid nitrogen cooling holder Gatan 655 pumping station JEOL JSM 840A W filament BSE detector Oxford Instruments Inca X ray analysis and SEM 1986 imaging system and 10 mm Si Li X ray detector with SATW window JEOL JSM 6300F Cold FEG Noran 10 mm HPGe X ray detector with UTW window and FEGSEM 1991 WinEDS X ray analysis and imaging system Removed from service 2013 x 3D AFFIM Kindbrisk 3D Atom Probe Field lon Microscope Removed from service 2015 3D Atom Probe Field lon Microscopes Oxford NanoScience 3D APFIM 2003 Oxford NanoScience 3D Atom Probe Field lon Microscope Specimen preparation equipment Allied Techprep polisher Atom probe tip polisher in house Branson Sonifier 450 Buehler EcoMet 3000 grinder polisher Buehler Handimet 2 roll grinder two units Buehler IsoMet slow speed saw Cressington 208 HH sputter coater Cressington 208 carbon evaporative carbon coater Ditabis Micron image
46. researcher intended for publication with a Monash co author There is no charge for undergraduate work Honours and 4 year projects prac classes etc In 2015 the internal charges are 40 hr booked or logged whichever is greater with a cap of 2 500 per Project per calendar year There is also a one off charge of 500 for new users requiring training There is no additional charge for any subsequent training or assistance or for reasonable use of specimen preparation equipment and consumables but the Centre reserves the right to recover costs where this is deemed necessary TEM users can purchase tweezers agate pestle and mortars and approved Cu grids with holey carbon film at cost from the General Office A basic set of ultramicrotome knives is provided for general use but regular ultramicrotome users may prefer to purchase their own Users may also need to provide their own diamond saw blades There is no charge for a reasonable amount of Client work carried out by a Centre staff member on behalf of a user other than for microscope time but the amount of this type of work that can be undertaken is limited This internal charge rate represents a small fraction of the real cost of maintaining and operating these multi million dollar instruments and of the cost of providing expert staff to train and support users The rate is very heavily subsidised by the University and is comparable or less than that charged by other Go8 universities
47. rus software 4 All files on the shared network drive are considered to be temporary it is your responsibility to ensure that you have secured your data The contents of the Shared_Data directory will be deleted at the end of each year and possibly at other times MICROSCOPE LABORATORY DOORS All microscope laboratory doors must be kept closed for the following reasons TEMs and older SEMs are operated with subdued or no lighting To ensure thermal stability of microscope rooms Microscope laboratories have special designs to enable better than 0 1 C hour to optimise microscope performance To keep out dust Microscope laboratories have special air flows and filters to minimise dust in order to protect the microscope and your specimens To provide acoustic isolation to prevent degradation of microscope performance by outside noise Room in use light While you are using a microscope you should turn on the Room in Use light If the Room in Use light is on you must knock before entering a room and wait to be admitted The microscope rooms have very good acoustic shielding and so you often will not be able to hear the microscope User s reply to your knock f the microscope User does not open the door you may not enter It is very important that you turn off the Room in Use light at the end of your session 35 MCEM User Manual 2015 SAFETY RULES AND LABORATORY PROCEDURES The Safety Rules and Labor
48. s labelled with the User s name telephone number date and composition where necessary can be left in designated areas within the specimen preparation laboratories for short periods while samples are actually being made It is assumed that anything left behind is not needed and after a short grace period these materials will be disposed of Keep benches sinks and fume hoods clean and clear of clutter If equipment is not functioning as you expect seek help 37 MCEM User Manual 2015 Fume Cupboards Work with the sash only open far enough to perform the procedure comfortably Do not leave any unnecessary glassware chemicals equipment in the fume cupboard Never use a fume cupboard if the extraction fan or backwash are not working Report any faults immediately to a Centre staff member Samples and Chemicals Materials Safety Data Sheets MSDS for each chemical stored or used in a laboratory are kept in the master MSDS folder located in room G35 or can be assessed online using ChemWatch from the computer located in Rooms G35 Ensure that you read the MSDS for all chemicals used by you and prepare Risk Control Worksheets and Safe Work Instructions as required A glossary of terms used in the MSDS forms is located in each folder Only those chemicals listed for each laboratory in the front of the master MSDS folder may be stored or used in the Centre You must have carried out a Risk Assessment for your work see www monash ed
49. s required may range from one or two for the simpler techniques such as sputter coating to eight or more for the more difficult techniques such as electropolishing and ion beam techniques In some cases you may need to contact other groups within the University to arrange access to specimen preparation equipment not available within MCEM such as polishing and grinding equipment operated by the Department of Materials Science and Engineering or the School of Earth Atmosphere and Environment Additional Training After you have completed your initial training and as your work progresses you may require additional training This could be in the application of more advanced techniques in the operation of other microscopes or in the operation of additional specimen preparation equipment To arrange additional training please contact the relevant Instrument Manager see Table 5 Refresher Training If you do not make regular use of licensed equipment your licence will lapse and you will not be able to make bookings or use the equipment The period of time after which your licence will lapse varies and is typically 3 to 6 months depending on the complexity of the equipment If your licence has lapsed you will need to take refresher training Please contact the Microscope Manager who will book a session for this refresher training The purpose of the refresher training is both to ensure that you Know how to correctly operate the equipment and also to inf
50. scopy on the Monash University Clayton campus 11 MCEM User Manual 2015 BUILDING DIRECTORY Plans of the ground floor and mezzanine level are shown in Figure 2 and Figure 3 respectively The Centre s room directory and telephone list is given in Table 1 AAA ame ne eee ee U eranda ad G02 a o Ei eere Entry gt Goz r GU9 t M a G03 G04 G05 GO6 Male Toilet Toe T G10 Meeting Room T mL Reception Copy Office Office JE G08 a Ej Kitchen j F I A FH fel lee A Es x G17 TEM Laboratory G42 SEM Laboratory S Bp itg Utility 524 Kn i G23 TEM Laboratory qe 9 gq A AA my fi G3 APFIM Laboratory G39 Corridor AL oy f l 2 i Figure 2 MCEM Building 81 ground level 12 MCEM User Manual 2015 LLL Corridor idu c EE REPE E 1 a ur Ne Bes a ER A A E 1 1 0 102 103 104 105 106 107 108 Research Fellows Office Computer Room Office Office Office Office Office Office E r a ES Ee a a a Figure 3 MCEM Building 81 mezzanine level Toilets Male Disabled and Female toilets are located in the administration area at the North end of the building top of the plan Rooms G07 G08 and G09 respectively The unisex toilet located on the mezzanine level
51. ssued a fob Business Hours bookings on microscopes can be made between 9 00am and 5 00pm on normal university working days Monash staff and students must display their Monash ID card while in the Laboratory Area card holders can be obtained from the MCEM General Office All Visitors must sign in and wear a Visitor Badge Only authorized personnel may enter a given room in the Laboratory Area unsupervised A list of authorized personnel is provided outside each room in the Laboratory Area Room access rights are summarised in Table 2 Table 2 Summary of room access rights EE NENNEN RH Microtome Microtome Laboratory Loensed microtome users microtome users EIB Sale hina abortos se und card access Electropolisher and P 9 y APFIM licensed users only opecimen Preparation Laboratory B MCEM staff only All Utility Rooms MCEM staff only Clean Workroom MCEM staff only 17 MCEM User Manual 2015 After Hours Access An After Hours licence may be granted once a microscope user has achieved a sufficient level of skill to be able to operate the microscope independently Additional training is provided to allow the User to correctly rectify minor issues should these arise after hours when MCEM staff are not available A minimum of six months regular trouble free microscope use is needed before a User may apply to the Microscope Manager for consideration for an After Hours licence After Hours access is only available to Mon
52. st not enter the laboratory Oxygen sensors and alarms Laboratories G30 G31 G35 G37 and G38 are fitted with oxygen sensors and alarms that will operate if the oxygen level drops below 19 5 normal reading 20 9 If a low oxygen level alarm sounds leave the room and advise a Centre staff member of the alarm If an Oxygen alarm is operating you must not enter the laboratory Oxygen levels can be monitored remotely on the control panel in Communications Duct Room G46A Liquid Nitrogen Liquid nitrogen is used in many parts of the Centre Liquid nitrogen can cause severe burns and the eyes are particularly vulnerable Always wear the apron full face visor insulating gloves and closed footwear when filling transfer containers from the liquid nitrogen storage vessels or when transferring and pouring 39 MCEM User Manual 2015 liquid nitrogen from the transfer containers Runners with mesh uppers and sandals are not acceptable when handling liquid nitrogen Never handle liquid nitrogen whilst wearing disposable gloves Liquid nitrogen boil off can displace oxygen so there is a risk of asphyxiation minimize spillage and decant in well ventilated areas The 160ltr Dewar must not be stored or used anywhere other than the Gas Store Room G38 which has direct external ventilation Thermal shock can cause materials to fracture only use the stainless steel or Nalgene Dewars provided or Dewars provided by equipment manufacturers to han
53. t to receive an email alert when a microscope becomes available through a cancellation This gives all people who urgently need microscope time an equal opportunity to take advantage of a cancellation Please note that the normal booking rules still apply At the bottom of the list of your details is a series of check boxes one for each microscope that you are licensed to operate If you tick a box you will automatically be sent an email whenever a booking on that microscope is cancelled Booking Rules The equipment in the Centre is in heavy demand Please observe these simple rules so that we can optimize access for everyone You must attend ON TIME every session you are booked for unless there are exceptional circumstances beyond your control such as illness If you are unavoidably delayed in starting a session you MUST contact the Instrument Manager or one of the Instrument Engineers Users who fail to notify the Centre of a delay within 30 minutes of their scheduled start time may have their session reassigned to another User Persistent lateness may result in a reduction or suspension of booking entitlements You must fill in the instrument logbook at the start and end of your session Note any unusual conditions or equipment problems Report serious problems in writing and in person to the Instrument Manager or Instrument Engineer Failure to fill in logbooks correctly can result in a reduction in your booking entitlement or lice
54. tropolishing and Cryogenic Microtoming are classed as hazardous procedures that are not allowed to be undertaken outside normal Centre business hours In the event of a fire alarm after hours make the equipment safe and evacuate the building on the first fire alarm Orange light Beep Beep alarm Do not re enter the building until you are advised that it is safe to do so by a University security staff member or Centre staff member Personal Protective Clothing And Equipment Closed footwear must be worn in all laboratories Additional Personal Protective Equipment e g laboratory coats long trousers protective aprons safety glasses full face safety visor gloves must be worn wherever indicated If you are wearing gloves to protect yourself from contamination electropolishing solutions film developer fixer etc please remove them before touching door handles or other communal items Thin blue disposable nitrile gloves are available for procedures involving the handling of specimens or photographic processing Leather gloves are available for processes involving liquid nitrogen and are not under any circumstances to be used for handling chemicals Personal protective clothing and equipment must not be worn in office areas or meals areas Personal protective equipment PPE zones in Specimen Preparation Laboratory G35 are indicated by the yellow tape markings on the floor All work completed within these zones requires the use o
55. tropolishing Manager 105 Assoc Prof Laure Bourgeois 990 55368 Office JEOL 2100F TEM Manager Dr Amelia Liu 990 58789 Office SEM 4 FIB Manager Dr Jisheng Ma 990 56250 Office Electron Microscopist David Vowles 990 53435 Microscope Engineer Dr Kathryn Waldron 990 53435 Electron microscopist Assoc Prof Matthew Weyland 990 59026 Office Titan Manager Unisex Toilet Mr Yueming Gao 59347 Fellows and Dr Hadas Katz Boon 99go0725 Aelunets Ones Professor Alex Moodie 990 58065 Mr Dan Nguyen 990 50259 Dr Chris Rossouw 990 56210 Dr Mike Walsh 990 20747 Professor Harry Whitfield 990 20723 Dr Changlin Zheng 990 24036 Dr Ye Zhu 990 54923 16 MCEM User Manual 2015 BUILDING ACCESS The main MCEM entrance is open from 8 45am to 5 30pm on normal University business days This gives access to the General Office Director s office Manager s office Kitchen and the Meeting Room Laboratory Area Access Access to the main laboratory area is restricted Authorised Centre Users can enter the Laboratory Area via the door in Reception from 8 45am to 5 30pm on normal University business days Monash staff and students can open the door to the laboratory area using their Monash ID card Please contact the General Office if you change your ID card so that we can contact Security to activate your new card to allow access to the building Visitors should go to the General Office to ask for the door to be opened or may be i
56. u au ohs forms risk management program pdf and you must inform MCEM of all Risk Phrases and or Safety Phrases associated with each material that you intend to bring into MCEM whether the material is a sample or is used for sample preparation Users MUST NOT bring any chemicals into the laboratory without permission Permission must be sought from the Safety Officer Dr Russell King or the Deputy Safety Officer Dr Peter Miller before any new chemical may be introduced into a laboratory The Centre will arrange for the supply and disposal of chemicals Store all chemicals in the appropriate cabinet Segregate different classes of chemicals refer to MSDS for class Label all chemicals reagents samples and wash bottles Labels on working solutions and preparations must include the chemical composition and product name the preparation date expiry date if appropriate and the owner s name and telephone number Waste solvents and expired washing methanol in G37 are to be placed in a 10L waste solvent container in the G37 flammable storage cabinet Waste solvents in G35 are to be placed in the 2 5L waste solvent container in the G35 flammable storage cabinet The following chemicals are strictly BANNED from the Centre Hydrofluoric Acid Perchloric Acid Acetic Anhydride mixes Nitric Acid Ethanol mixes Electropolishing solutions are potentially explosive and must be handled under highly controlled conditions to minimize the risk of explosio
57. ulate form For hazardous substances the high surface area and number density of the ENP form may mean that safe exposure limits based on weight may not be appropriate Inhalation ingestion and permeation through damaged skin or wet membranes ie eyes are the most likely routes into the body Handling ENPs Dry ENPs must be handled inside a fume cupboard to prevent inhalation Gloves and safety glasses must be worn when handling dry or liquid suspensions of ENPs to prevent skin or eye contact Emergency Stop buttons Laboratories G30 G31 G33 G35 and G37 are fitted with an Emergency Stop button Pressing this button will cut all power to GPOs in these five rooms Fume cupboards are also fitted with Emergency Stop buttons that will cut power to that fume cupboard UPS power All main microscope power outlets and power outlets for water chillers in Utility rooms are supplied from uninterruptible powers supply UPS units located in the electrical plant room Red general power outlets GPOs in microscope rooms and in the General Office are also connected to UPS UPS power is provided in the Research Fellows Office for the Communications Rack two outlets at high level and to the floor box closest to the communications rack SF sensors and alarms All TEM microscope laboratories are fitted with SF sensors and alarms If an alarm sounds leave the room and advise a Centre staff member of the alarm If an SF alarm is operating you mu
58. utton Your new booking should then appear on the re displayed day bookings table An email confirmation of the booking will also be sent to you Log out when you have completed your booking How To Cancel An Online Instrument Booking After login select Current bookings and click on the View Bookings button This will display a list of your current future bookings for all instruments Click in the Action Cancel column for the booking to be deleted Select the number of hours that you wish to cancel To complete the cancellation press the Delete Booking button Your cancelled booking should then be erased from the re displayed day bookings table An email confirmation of the cancellation will also be sent to you You can cancel a booking at any time up to one day prior to the start of the session To cancel a booking within one day of the session you will need to contact the Instrument Manager or Centre Manager Please note that we expect that a late cancellation will be a very rare event perhaps once or twice a year People who make an excessive number of late cancellations may have their booking rights reduced A log is kept of booking cancellations 31 MCEM User Manual 2015 CHARGES Internal charges The internal charge rate applies to 1 all microscope use by Monash staff and students postgraduate or undergraduate who are working on internal University projects 2 all collaborative work with an external
59. w local data folder is created each year and all data in the previous year s folder is deleted It is up to you to move any data that you wish to keep into the current year s folder As mentioned above USB memory sticks are not permitted You can write your data to DVD or CD or you can transfer your data to your computer via the shared N drive see instructions on the MCEM web page at http www mcem monash edu au embookings docs net_drive_setup pdf Rules of use for the shared network N drive 1 The shared network drive is only to be used for transfer of data generated within MCEM between an MCEM computer and your computer 2 Each microscope has its own folder in the Shared_Data folder where you can create a sub folder to hold your data The sub folder name must be your first name or initial and surname followed by your Project Number so that we can identify the owner of the data Folders that do not conform to this format or files created anywhere else including the desktop will be deleted Do not create folders elsewhere 3 The shared network drive must not be used for data storage All files on this drive are accessible to all MCEM users around 400 people and potentially to anyone in the 34 MCEM User Manual 2015 university These files are not secure or confidential and an unintentional or deliberate act could result in the loss or corruption of some or all of this data The shared network drive may also contain vi
60. y G26 NENNEN MCEM staff only Dr Flame Burgmann 990 56211 Office SEM Manager Microtome Laboratory 990 58790 Licensed users only G27 G29 G30 Mr Selwyn Glanvill 990 20822 Ultramicrotomist TEM Support Laboratory 990 58777 TEM licensed users only 990 20717 TEM licensed users only Clean Workroom 990 20718 MCEM staff only Dr Russell King Office Microscope Engineer 990 53804 Safety Officer G31 G32 G33 Mr Renji Pan 990 54931 Microscope Engineer Specimen Preparation 990 54905 All licensed users Laboratory A Lt 990 20719 Licensed users only Electropolishing Laboratory 990 20701 Electropolisher licensed users only Liquid Nitrogen and Gas Store MEN All licensed users JEOL 7001F FEG SEM 990 20702 Licensed users only FEI Magellan 400 FEGSEM 990 20703 Licensed users only FEI Nova NanoSEM 450 990 20704 Licensed users only FEGSEM Go K G35 G36 G37 G38 G41 G42 G43 44 Utility Room Laboratory G43 MCEM staff only Utility Room Laboratory G42 MCEM staff only G46 Utility Room Laboratory G41 MCEM staff only G47 Utility Room Laboratory G36 102 Computer Room 990 20723 All licensed users 103 Dr Tim Williams 990 20721 Office Electron Microscopist Tecnai TEM Manager DENN MCEM staff only 15 MCEM User Manual 2015 Room Dr Emily Chen 990 55348 Office Electron Microscopist and Specimen Preparation Specialist Dr Xi Ya Fang 990 20821 Electron Microscopist and Elec

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