Leading the way to healthy crops. USER GUIDE: Agdia
Contents
1. Q agdia Leading the way to healthy crops USER GUIDE Agdia ImmunoStrip Test KIT INFORMATION Intended Use The ImmunoStrip test is a rapid means of screening crops for the presence of pathogens ImmunoStrip tests require no equipment or expertise to run Results are obtained in as little as a few minutes making them perfect for use Agdia Inc 30380 County Road 6 Elkhart IN 46514 Phone 574 264 2615 or toll free 800 622 4342 Fax 574 206 9360 Web www agdia com Email info agdia com ImmunoStrip Kit ISK Includes ImmunoStrips e SEB1 sample extraction bags e User guide in the field or greenhouse The ImmunoStrip must be used with SEB1 sample extraction buffer for leaf Do not use any other sample extract buffer Storage of Kit ImmunoStrips should be stored refrigerated 4 C between uses and tightly sealed in the desiccated container at all times Kit contents should be warmed to room temperature prior to use PERFORMING THE ASSAY Special Attention Required Prepare Sample Collect a sample section that is approximately 1 inch square in size It is recommended that samples be taken from areas of the plant that exhibit symptoms of disease Note It is recommended that you use a clean cutting tool for each sample If you must reuse the cutting tool first wipe off the cutting edge and disinfect in a 10 bleach solution before cutting into a new sample Cut open the sample extraction bag along t2. f pink purple this indicates a positive result Positive Negative If no lines are present the test is invalid see troubleshooting SAFETY ImmunoStrips and sample extraction buffer are non hazardous TROUBLESHOOTING Control line did not develop Test runs very slow or not at all Test has a green or pigmented test line Test and or control line is weak m175 1 This situation is generally caused by over submergence of the test strip in the sample extract Results in this situation should be considered invalid and the test should be repeated This can be caused by using too much tissue for extraction Repeat the test using less tissue or by diluting your sample extract 1 10 with SEB1 If the above is not the case make sure the test components were warmed to room temperature before use and are within their expiration date This can be caused by using too much tissue for extraction Repeat the test using less tissue or by diluting your sample extract 1 10 with SEB1 In rare cases the tissue type may cause a pigmented line Green lines should not be considered a positive result Red orange or purple fruits may cause what appears to be a positive test line It is recommended that you contact Agdia before testing these types of samples Make sure the test is within its expiration date If kit contents were left open too long the strips could have absorbed moisture which can affect test results Be sure
3. he top of the label Be careful not to spill the buffer SEB1 Buffer is required to perform this assay with leaf tissue Insert the sample between the mesh linings near the bottom of the sample extraction bag Extract the sample by rubbing it gently between the mesh linings with a blunt object such as a pen or permanent marker Depending on the sample type the color of the solution will turn a light brown or green color once the sample is adequately extracted m175 1 ImmunoStrips STX purchased separately do not include buffer filled mesh bags What s required to perform the assay e Scissors knife or razor blade e SEB1 sample extraction buffer e Sample extraction device Agdia sample extraction bags are recommended e Letter holder or other device to hold sample extraction bags Perform Assay Insert the ImmunoStrip into the channel portion no mesh of the buffer filled bag Be sure to insert the sample end of the strip no more than or to the white line on the ImmunoStrip label Allow the ImmunoStrip test to remain in the sample extract for 30 minutes Positive results may be visible in as little as 5 minutes Lower titer samples may take up to 30 minutes T Interpret Results Remove test strip from extract and interpret results see illustration If only the control line C is visible this indicates a negative result If the test line T is also present at any intensity o
4. to always keep the ImmunoStrip vial tightly sealed between uses The test line may be weak due to low pathogen titer in the sample
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