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1. n Xi Balance Reading number or reading Conversion factor example 7 1 0040 ul mg at 25 c and 1013 hPa NI Calculation for in accuracy Systematic Error A V Vo 100 Vo V Mean Value Vo Particular volume at which readings are taken Calculation for Impression Random Error S Standard Deviation IVi VP C 1 V Mean Value 1 n of readings CV 100 5 V Compare the results to the limits in the subsequent tables 10 1111 SMART PIPETTE USER GUIDE Variable Volume Pipette CAT No al lb ds 27 ull 4 4 0 25 12 6 SVA 125 O 0 2201 0 002 3 3 2 5 210 1 6 1 2 5 1 8 SVA 100 e 0 5 10 ul 0 02 5 2 1 10 1 0 6 5 2 2 SVA 200 5 90 ul 0 1 25 0 8 0 4 50 0 6 0 3 10 2 1 SVA 500 10 100 pl 0 1 50 1 05 100 0 8 0 2 100 9 0 6 SVA 600 100 1000 ul 0 1 500 1 0 4 1000 0 6 122 2 3 2 SVA 700 O 2 20 ul 0 02 10 1 5 1 20 0 9 0 4 20 2 9 0 7 SVA 800 20 200 ul 072 100 Ors 200 0 6 0 2 500 2 5 0 6 SVA 905 O 500 5000 ul 10 2500 1 2 0 25 5000 0 6 0 15 1000 3 0 6 SVA 1000 1000 10000 ul 20 5000 0 8 12 10000 0 6 022 11 SMART PIPETTE USER GUIDE WEN Fix Volume Pipette Test Color Volume Range i Inaccuracy
2. Imprecision CAT No Volume code ut 96 96 ul sFa 2 5 2 5 pl 2 5 2 1 6 SFA 0005 5 pl 5 i3 1 2 SFA 0010 10 ul 10 12 0 8 SFA 0020 20 ul 20 1 0 5 SFA 0025 25 pl 25 1 0 3 SFA 0050 50 pl 50 0 7 0 3 SFA 0100 100 pi 100 0 6 0 2 SFA 0200 200 pl 200 0 6 0 2 SFA 0250 250 pl 250 0 6 0 2 sFA 0500 500 pl 500 0 6 0 2 1000 1000 pi 1000 0 6 0 2 sFA 2000 2000 pi 2000 0 3 0 15 5000 5000 pl 5000 0 3 0 15 sFa 10000 10000 pi 10000 0 6 0 2 Multi Channel Pipette 8 Channels amp 12 Channels Test Volume Incre Inac Impreci Color Vol ments curacy sion code pg qu UM ti gu 8 SMA8 010 sma12 010 0 5 10 pl 0 02 5 4 2 10 2 2 7 3 SMA8 020 2 20 0 02 10 3 2 20 2 1 6 5 3 2 SMA8 050 fn O 5 50 ul 0 1 25 1 5 1 50 1 0 7 10 3 2 SMA8 100 10 100 0 1 50 1 0 8 O pl 100 0 8 0 3 12 1111 SMART USER GUIDE 20 5 1 4 SMA8 200 20 200 amanan O 0 2 100 1 0 4 200 0 7 0 25 30 3 1 SMA8 300 30 300 0 2 150 0 5 300 0 6 0 3 Calibration Adjustment 1 Calibration adjustment is done with the Recalibration Ellen key 2 Remove the calibration guard and place the Recalibration Ellen key into the opening of the calibration screw on the side of the handle 3 Turn the key clockwise to increase or counterc
3. Release the operating button to the ready position Change the tip and continue pipetting Reverse Pipetting The reverse technique 15 suitable for dispensing liquids that have a high viscosity or a tendency to foam easily The technique is also recommended for dispensing very small volumes 1 Press the operating knob all the way to the second stop 2 Dip the tip under the surface of the liquid in the reservoir to a depth of about 1cm and slowly release the operating knob This action will fill the tip Withdraw the tip from the liquid touching against the edge of the reservoir to remove excess liquid 123 4 3 Deliver the preset volume by gently pressing the operating knob to the first stop Hold the operating knob at the first stop Some liquid will remain in the tip and it should not be dispensed 4 The remaining liquid should either be discarded with the tip or should be dispensed back into the reagent reservoir 8 111 SMART PIPETTE USER GUIDE CALIBRATION AND ADJUSTMENT All SMART pipettes are been quality tested according to 1S08655 DIN 12650 The quality control according to ISO 8655 DIN 12650 involves gravimetric testing of each pipette with distilled water quality 3 DIN 1503696 at 25 C 1 All SMART pipettes are been calibrated in ISO IEC 17025 accredited laboratory Each Pipette is calibrated inspected and validated by qualified technicians according to de
4. volume range is not advisable Using excessive force to turn the volume changing knob outside the specified range may jam the mechanism and eventually damage the pipette 6 111 SMART PIPETTE USER GUIDE Loading and Ejection Tips Each SMART pipette 15 fitted with a tip ejector which helps eliminate safety hazards associated with contamination It is necessary to check that the tip cone is clean before fitting a tip Press the tip on the cone of the pipette firmly to ensure an airtight seal The seal is set tight when a visible sealing ring forms between the tip and the tip cone The tip ejector needs to be pressed downwards firmly with the thumb to ensure proper tip ejection Once the process is complete make sure that the tip 15 disposed of into a suitable waste container PIPETTING GUIDELINES e While using the pipette make sure that operating knob has been handled slowly and smoothly e Ensure that the tip is firmly attached on the tip cone Please check for foreign particles and remove if any around the tip cone e Make sure that the temperature of tip pipette and liquid are at equilibrium e While holding the pipette its main handle should be resting on your index finger e While aspirating hold the pipette in upright position and keep the tip at a constant depth below the surface of the liquid e You should pre rinse the tip before aspirating the liquid by filling and emptying the tip 5 times This is imp
5. A Fine Care Biosystems WWW accumaximum com SMART PIPETTE USER GUIDE 1111 INTRODUCTION Congratulations on making a SMART choice You are now the proud owner of an Accumax SMART pipette This user guide lists details on how you can take care of your SMART pipette and benefit the most from its advanced features as follows e SMART 15 one of the most lightweight pipette with durable working e Designed to dispense accurate volume of liquid with long term precision e Ergonomically designed for ease In operation and user safety Color coded for easy identification CHECK LIST Your Accumax SMART pack would carry e SMART Pipette e Sample pack of tips Recalibration Ellen Key e Silicone Grease e Shelf Mounting Stand e User Guide e Warranty Card e Certificate of Conformity including Calibration Certificate PRODUCT DESCRIPTION SMART pipettes operate on air displacement principals and uses disposable tips Variable volume pipette CAT No Volume Range pl Increments ul SVA 125 0 1 2 5 0 002 SVA 100 0 5 10 0 02 SVA 200 5 50 0 1 SVA 500 10 100 0 1 SVA 600 100 1000 1 SVA 700 2 20 0 02 SVA 800 20 200 077 SVA 905 500 5000 10 SVA 1000 1000 10000 20 2 111 Fix Volume pipette SMART PIPETTE USER GUIDE CAT No Volume pl SFA 205 2 5 SFA 5 D SFA 10 10 SFA 20 20 SFA 25 25 SFA 50 50 S
6. FA 100 100 SFA 200 200 SFA 250 250 SFA 500 500 SFA 1000 1000 SFA 2000 2000 SFA 5000 5000 SFA 10000 10000 Multichannel pipette 8 channels CAT No Volume Range ul Increments pl SMA8 010 0 5 10 pl 0 02 SMA8 020 2 20 ul 0 02 SMA8 050 5 50 pl 0 1 SMA8 100 10 100 pl 0 1 SMA8 200 20 200 ul 0 2 SMA8 300 30 300 ul 0 2 12 channels CAT No Volume Range ul Increments pl SMA12 010 0 5 10 pl 0 02 SMA8 020 2 20 ul 0 02 5 12 050 9 50 pl 0 1 SMA12 100 10 100 pl 0 1 5 12 200 20 200 pl 0 2 SMA12 300 30 300 ul 0 2 3 SMART PIPETTE USER GUIDE Digital Display In SMART pipette delivery volume 15 evidently indicated in the large digital display on the handle In variable volume pipette the last wheel of the counter has scale that assists to set precise delivery volume In some of the variable volume pipettes the last one or two counter wheels will be in grey color which indicates the value after decimal A pointer is used to set exact or intermediate volumes with the help of scale on last wheel Raw Material The SMART pipette is made of mechanically robust and chemically resistant materials making it a durable and stable pipette giving consisting performance for long time 4 SMART PIPETTE USER GUIDE Parts Description ASPIRATION DISPENSING KNOB OPERATING VOLUME CHA
7. NGING KNOB TIP EJECTOR PUSHER COUNTERLOCK COUNTER WINDOW COVER COUNTER WHEELS MAIN HANDLE COMFORT GRIP MAIN HANDLE TIP EJECTOR CLOSER TIP EJECTOR TIP CONE 111 5 SMART PIPETTE USER GUIDE PIPETTE OPERATION Setting the Volume With the SMART pipette the delivery volume of liquid is set using the digital counter A pointer is used to set exact or intermediate volmes using the scale on the last wheel of digital counter In some of the variable volume pipettes counter wheels are in black amp grey color Grey wheel indicates value after the decimal Locked Position Un locked Position Push up to un lock Pull down to Lock You can set the delivery volume using the volume changing knob on the upper side of pipette after unlocking the counter by pulling up the counter lock To decrease the delivery volume turn it clockwise and to increase turn Volume it anti clockwise Do make sure 27 that the desired delivery volume 15 set in line with pointer Pull down the lock to ensure that volume doesnt accidently changed during pipetting 1 Counter lock mechanism 16 to ensure volume changing knob remains in ts position while aspirating or dispensing liquids Any forced efforts to rotate the volume changing knob while counter lock mechanism 15 in lock position will either make it in unlock position may damage the locking mechanism 2 Setting the volume beyond the specified
8. Volume 1 10ml Fix Volume 10ml e SP 30 2 gt SP 50 1 e SP 52 1 SP 353 1 SP 57 1 SP 262 1 SP 43 1 7 SP 29 1 SP 27 1 SP 312 1 SP 55 1 AUTOCLAVING SMART PIPETTE USER GUIDE Accumax SMART pipette 15 completely autoclavable at 2 bar pres sure 121 temperature for 20 minutes exposure time according to DIN e Keep digital counter in unlock position e Autoclave the complete pipette without any disassembling e After autoclaving allow pipette to completely cool and dry If the pipette is autoclaved frequently the piston and springs should be greased with the lubricant supplied with each pipette to maintain smooth movement TROUBLE SHOOTING GUIDE Possible Cause Solution Problem area Pipette is leaking Worn O ring seal Replace worn parts Foreign particles between tip and tip cone Clean tip cone attach new tip Foreign particles between piston and seal Clean seal and piston Pipette wont Worn O ring or seal Replace worn parts dispensing with certain liquids particular liquid aspirate Tip cone 1 loose Tighten tip cone Piston is damaged Return pipette to Chemically or Mechanically Authorised Distributor Damaged tip cone Replace the tip cone Pipette is Improper assembly See Maintenance inaccurate section Tip cone Is loose Tighten tip cone Tip incorrectly attached Attach f
9. fined quality system SMART pipettes are constructed to permit re adjustment for other pipetting techniques or liquids of different temperature and viscosity Devise requirements and test conditions An analytical balance must be used The balance selection depends upon selected model of the pipette and sensitivity of balance reading Test liquid Water distilled or deionized grade 3 water conforming 503670 Calibration should be carried out in a draft free room constant 0 5 C temperature of water pipette and air between 15 C to 30 C The relative humidity must be above 50 especially with volumes under 504 the air humidly should be as high as possible to reduce the effect of evaporation loss Special accessories for analytical balance such as the evaporation are recommended for the calibration of volumes under 50 111 9 SMART PIPETTE USER GUIDE Procedure to check calibration The pipette is checked at maximum volume at 50 of maximum volume and at minimum or 10 of maximum volume whichever is higher e Anew tip is first pre wetted 3 5 times and series of ten pipetting is done at each volume e Use of forward pipetting technique is recommended e Calculate the inaccuracy and imprecision for all three volumes as per EN ISO 8655 DIN 12650 standards on the basis of the following calculation Conversion of weight readings to volume Mean Value Z Mean Value X Xi
10. irmly Calibration altered Recalibrate according to instructions Inaccurate Calibration not suitable for Recalibrate with the liquid in question 21 A Fine Care Biosystems Smart GEN NEXT PIPETTE WWW accumaximum com
11. lockwise to de crease the volume 4 After adjustment check the calibration according to the instruc tions above 9 Once within permissible error range place calibration guard its position to avoid accidental changes in calibration For more detail information about Calibration and Adjustment visit www accumaximum com calibration Depending upon use we recommend checking of calibration every six months However this can be adjusted to individual requirements 13 SMART PIPETTE USER GUIDE 1111 MAINTENANCE When the SMART pipette 15 not in use please make sure that it 1 stored in an upright position Accumax recommends the SMART stand for this purpose You should check the pipette at the beginning of each day for accu mulation of dust and dirt on the outside surfaces Attention should be paid to the tip cone No other solvents except Iso propanol should be used to clean the pipette If the pipette is used daily it should be inspected every three months The servicing procedure starts with the disassembly of the pipette Please refer to the spare parts lists for better understanding of the components Shown here 15 an example of dis assembly and as sembly procedure of 100 1000 ul pipette the basic procedure is to be followed for all other volume pipettes y 1 For opening press 2 Rotate the tip ejector 3 Turn out the tip ejector the tip ejector closer closer in the direction slowly upwards indica
12. me 0 5ul 2 51 41 1 301 1 SP 48 2 o SP 258 1 5 48 1 SP 38 1 A SP 25 1 SP 314 1 SP 14 1 SMART PIPETTE USER GUIDE Group 3 Variable Volume 0 5 10 1 Fix Volume 10pl SP 41 2 SP 301 1 SP 48 2 SP 259 1 SP 48 1 SP 38 1 12 SP 25 1 SP 314 1 2 ES 17 SMART PIPETTE USER GUIDE Group 4 Variable Volume 2 20ul Fix Volume 20ul SP 37 3 301 1 SP 48 2 SP 260 1 SP 48 1 SP 38 1 m SP 25 1 SP 14 1 18 Group 5 Variable Volume 5 50 Fix Volume 25pl 50 SP 40 1 SP 306 1 SP 45 1 SP 391 1 SP 265 1 SP 47 1 SP 38 1 SP 24 1 SP 14 1 Group 6 Variable Volume 10 100 Fix Volume 100 1 SP 39 1 SP 303 1 SP 45 1 SP 386 1 SP 261 1 e SP 46 1 gt SP 38 1 SP 24 1 SMART PIPETTE USER GUIDE Group 7 Variable Volume 20 2004 Fix Volume 200ul SP 366 1 SP 305 1 SP 45 1 SP 388 1 264 1 SP 45 1 SP 38 1 SP 24 1 19 SMART PIPETTE USER GUIDE Group 8 Variable Volume 1 5ml Fix Volume 2ml 5ml SP 31 2 SP 51 1 1 SPA SP 304 1 SP 57 2 SP 42 1 SP 353 1 SP 263 1 SP 28 1 SP 26 1 SP 313 1 SP 54 1 20 Group 9 Variable
13. ortant especially when dispensing liquids which have a viscosity and density different from water e For the volatile solvents you should saturate the air cushion of your pipette by aspirating and dispensing the solvent repeatedly before aspirating the sample e When pipetting liquids with temperatures different to the ambient temperature pre rinse the tip several times before use e After pipetting acids or other corrosive liquids that emit vapors remove the tip cone and rinse the piston O ring and seal with distilled water e Do not pipette liquids having temperatures above 70 C or below 270 Make sure that liquids never enter the tip cone to prevent this e Never lay the pipette on its side when there 16 liquid in the tip e Press and release the push knob slowly and smoothly e Never turn the pipette upside down 111 T SMART PIPETTE USER GUIDE PIPETTING TECHNIQUES Forward Pipetting 1 Press the operating knob to the first stop 2 Dip the tip under the surface of the liquid in the reservoir to a depth of about 1cm and slowly release the operating knob Withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess liquid 3 Dispense the liquid by gently 1234 pressing operating knob to the first stop After a delay of i 30 about one second continue to press the operating knob all the way to the second stop This ac tion will empty the tip 4
14. ted until the snap e Pull out the piston and other parts by unscrewing the tip cone Push out with piston the rest of the piston assembly including the Seal and O ring Remember to keep all parts in order for reassem bly e Clean the piston the piston spring Seal and the O ring with a 150 propanol and lint free tissue Allow them to dry e Check the tip cone for foreign particles and remove if any e Grease the cleaned parts with the approved lubricant provided with each pipette 14 1111 SMART USER GUIDE 1 Align the notch on the tip ejector with the slots on tip ejector closer as indicated 2 Ensure the notch on the main handle matches the slots on the tip ejector closer Push the tip ejector closer upwards as indicated 3 Insert both parts in the aligned manner as indicated 4 Once completely closed twist the tip ejector closer in the indicated direction to close For correct re assembly it is crucial that the slots on the tip ejector closer match the notches on the inside ofthe main handle Please follow the above procedure SMART PIPETTE USER GUIDE List of Spare Parts Group 1 Variable Volume 100 100 Fix Volume 250pl 500pl 1000pl SP 377 1 SP 302 1 or SP 70 1 SP 23 1 SP 317 1 SP 392 1 SP 259 1 SP 44 1 SP 314 1 SE lt SP 14 1 16 1111 Group 2 Variable Volume 0 1 2 5pl Fix Volu

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