VU-AMS 5fs short user manual
Contents
1. Starting form the original 5fs file the various programs in this suite will create new data files that in turn become input for other programs in the suite Filenames will be maintained from the original filename given to the 5fs raw data file but extensions will change depending on the type of information produced A schematic of the various programs and the file extensions they produce is given below VU AMSStfs software suite CF N AmsPreProcessor AmsQRS AmsGraph card fs converting amsinv peak detection amsinv visualization Output Output N Output camsinv SECG beh gt beat Sxxxbeh X _ SECG bch 3 na Use gt File gt peat SDZ bch Import ECG SDZDT beh P i Z0 bch SSCL bch Etc 7N sts amsinv xxx bch beat AmsMerge Se gj g ee Mersin AmsReverFormat A Output Reverting Multiple 5fs imersed 5fs a Output i ams AimsConfigure Sei Gi aes Configuring the ormat VU AMS5fs device VU AMS 5fs short user manual The typical flow of events is xj AmsMerge AmsPreProcessor AmsQRS R wave detection AmsRevertFormat to ams AMSGRA data labeling amp HR RMSSD AMSRES RSA scoring AMSIMP PEP scoring File size 357870 bytes Subject ID 0603001 Started at 2007 03 13 12 02 13 Stopped at 2007 03 13 12 03 39 events 0 blocks 1 D Oy Oi e S Be AmsMerge If you hav2. the black ECG electrode first more laterally then more medially until a satisfactory QRS complex is seen The dZ should be within 0 5 and 0 5 Ohm most of the time ZO should always stay within an 8 to 20 Ohm range The dZ signal should reflect deep breathing clearly In the ICG the typical upward waveform of the cardiac ejection phase should be clearly detectable Light movement of the subject should not overly distort it If these criteria are not met re attach the electrodes in the order 7 6 1 3 4 5 2 until satisfactory signals are obtained The Scrollbar on the Y axis can be used to scale the signals your only opportunity to re attach faulty electrodes When satisfied start data recording by pressing the start button A beep will be heard to acknowledge the start of the recording and the green light will start flashing once every three seconds Close the AmsConfigure program You may now disconnect the VU AMS device from the interface Synchronize the watch of the subject observer to the exact time of the PC used to start up the VU AMS for optimally time locked self report diary and physiological data When electronic diaries are used make sure that their clocks are synchronized with this PC too Measurements can be stopped in the field by pressing the button for three seconds or longer the light will flash every 10 seconds to indicate stand by mode Once the VU AMS has stopped you may disconnect the lead wire plug s from th
3. VU AMS 5fs short user manual User Manual Ambulatory monitoring with the VU AMS version 5fs Requirements Two AA batteries Compact flash card Compact flash card reader Electrodes Lead wire connector VU AMS 5fs VU AMS for RS232 or USB Flashcard with latest firmware optional Software Use 1 2V rechargeable NiMH batteries or non rechargeable 1 5V alkaline batteries Make sure the bottom contact sticks out in some batteries it is the covered by an outer plastic ring these won t work properly with the VU AMS Leave rechargeable batteries in the loader up till the very last moment External memory card The VU AMS 5fs has been extensively tested with the 1GB 80x CompactFlash card from Transcend TS1GCF80 Other CompactFlash cards should work too Card reader unit to extract the AMS data from the CompactFlash card after recording and to erase the card for a next recording Any brand or built in CompactFlash card reader will do Seven electrodes are needed for a single recording We use the UltraTrace single use clear tape ECG electrode with Wet Gel A blue lead wire connector with 7 lead wires is used for the recording of the ECG and thorax impedance Optionally a second yellow connector for skin conductance recording is needed The ambulatory recording device An infrared interface cable that either connects to the RS232 serial port of a PC or to an USB port Typically the VU AMS comes w
4. able use a clean dry tissue Attach an electrode by pressing the sticky plastic brim of the electrode on the skin and subsequently pushing the metal stud at the center of the electrode firmly to properly spread the contact gel ECG 1 V Slightly below the right collar bone 4 cm to the right of the sternum 2 GND Onthe right side between the lower two ribs 3 V Under the left breast 4 cm 1 5 under the nipple ICG electrodes ICG 4 I At the back on the spine at least 3 cm 1 above electrode 6 OAE som 5 I At the back on the spine at least 3 cm 1 below electrode 7 6 V At the top end of the sternum between the tips of the collarbones aetan 7 V At the low end of the sternum where the ribs meet i en VU AMS 5fs short user manual Attachment of the lead wires and lead wire connector Attach the lead wires to the electrodes according to the color coding scheme in the figure above Next the blue ECG ICG lead wire connector has to be plugged in the blue socket Starting the measurement by plugging in The VU AMS device is always standby Measurement will re start after you plug in the lead wire connector and press the event button for three seconds or longer A beep will be heard to acknowledge the start of the recording and the green light will start flashing about once every three seconds Wearing the device Put the VU AMS device in its carrier bag with the lead w
5. ctFlash card Motility fi 10 Hz z Estimated memory usage per hour 13 9MB PEG ort B Memory available 990 6MB aap Ponci boar orf zl Estimated recording time left 71 4H VU AMS 5fs short user manual Check the battery type battery voltage indication should be about 3 4 Volt for alkaline and about 2 4 Volt for rechargeable NiMH batteries and re check time and date Fill out the identification field NB If you intend to use our preprogrammed SPSS scripts make sure the identifier is exactly 7 characters long Try to use a numerical name only e g 0603001 The typical sampling frequencies are as shown in the figure AmsConfigure allows you to set sampling frequencies for the various signals You can also disable signals here by setting them to Off When you change the any setting make sure to send the settings to the device before closing the screen Attach the electrodes as explained in the instruction leaflet How to attach the VU AMS version 5fs Appendix 1 After connecting the ECG ICG lead wire plug the Online option of the AmsConfigure program should be used to display the ECG dZ respiration and dZ dt ICG A clear QRST complex should be detectable in the ECG The R wave should be upward and it should be the peak with the largest absolute amplitude in either direction If either S wave or T wave are of NOTE In ambulatory paradigms this is comparable magnitude _ re attach
6. d under the Help option of the AmsQRS program basis of many other variables extracted from the VU AMS 4 AmsRevertFormat After corrections with the AmsQRS program the AMS 5fs signals can be converted to the old VU AMS file format of the VU AMS 4 6 series ams with the AmsRevertFormat program The immediate advantage of this is that all existing software for labeling AMSGRA and impedance scoring AMSIMP and respiration scoring AMSRES is now available The old VU AMS 4 6 series did not yet combine ECG SCL and ECG ICG in a single device You will have to choose the appropriate signal at the AmsRevertFormat open screen For instance to create an ams file with ECG ICG signals use the following settings in AmsRevertFormat VU AMS 5fs short user manual AmsRevertFormat Select file s to convert x Look in 06171445 e ee Fe My Recent Documents Desktop 06171445 DzZ bch 9 My Documents on gr My Computer am cinta File name X Places ams ICG Files D2Z bch AMS ICG Files Z bch AMS SCL File CL bch AMS beat Files beat All Files Files of type Cancel 5 AMSGRA data labeling amp HR RMSSD Before using any of the VU AMS programs that extract physiological parameters like AMSRES and AMSIMP it is important to first create a so called label data file default extension Ibl The aim of data labeling is to classify all activities o
7. d when this option is available For the heart rate HRA motility MOT and root mean squared successive differences of the heart rate MSSD settings can be changed by clicking on the colored box of the activated variable By ticking the option visible on or off a choice can be made which signals are displayed in the graphical panels HR and MSSD signals are highly sensitive to posture and physical activity To obtain information about a subject s daily routine all subjects have to keep a detailed diary during the ambulatory measurement day This diary information about type of changes in posture and activity is used during the labeling procedure The VU AMS device also contained an accelerometer which is sensitive to changes in vertical acceleration MOT signal This motility signal can be used to confirm the activities and postures written down in the diary The actual labeling procedure is done in the lower graphical display Use your left mouse button to select the start and end point of the new label you want to add Releasing the mouse button shows which time period is covered by your new label This label is still red chequered Click on the appropriate Category and Code names for this time period to finish the label The label is now red Repeat this procedure until the whole ams is labeled Close AMSGRA after you have labeled your ams file A label file Ibl will be saved This lbl file is an ASCII file In the lbl fil
8. e recording in the top panel and the raw ECG signal in the lower panel The middle panel also displays the IBI time series but only for the interval selected in the top panel VU AMS 5fs short user manual if 0603001_03131202 beat AmsQRS 0 x File Edit view Help Cea Bele e 04 17 10 01 17 20 01 17 30 01 17 50 01 18 00 01 18 10 T T v v T v m v T v v T 01 17 20 01 17 30 01 17 40 tine 01 17 50 Ot 18 00 01 18 10 gt EE 4 O w 01 17 21 01 17 22 time 01 17 23 01 17 24 01 17 25 Clicking in the top panel will automatically scroll the middle and lower panel to the corresponding point in time Visual inspection of the top panel will rapidly identify spikes which can occur because an R wave was missed and or a T wave was used instead The spikes can be corrected in the lower window by dragging the cursor to the correct R wave peak A single left click in the ECG panel will automatically insert a new R peak cursor at the selected location A single right click on an existing cursor will remove that cursor The left and right arrow keys of the keyboard allow you to step through all scored R peaks The active blue cursor can also be deleted by pressing the delete key The SO eee MME pe ee eee ent NOTE All time spent correcting the IBI the beat nies Detailed time series is well spent since it is the information on how to tailor pent automatic scoring is foun
9. e an average HR IBI MSSD and MOT value is given for each labeled time period Therefore the Ibl file can be used for further statistical analyses in for example in SPSS 7 8 AMSRES RSA scoring amp AMSIMP PEP scoring Separate manuals for impedance scoring AMSIMP and respiration scoring AMSRES are available on the website www vu ams nl VU AMS 5fs short user manual Trouble shooting AMS file has zero bytes iolxi File Edit View Favorites Tools Help Qx ts i Bi P Search gt Folders Ez Address E gt Go a Name Size Type Date Modified File and Folder Tasks za h 06251511 5F5 OKB 5F5 File 25 6 2009 15 11 Make a new folder Publish this folder to the Web E3 Share this Folder Cause the VU AMS has made a recording and probably all data are there but an end of file marker has not been placed Solution This can be repaired by closely following the instructions on www vu ams nl gt Download gt Video manual How to repair a OKB file recorded with the VU AMS 5fs Alternatively you can send the flashcard back to us VU AMS 5fs short user manual Appendixi1 How to attach the VU AMS 5fs 4 ICG l 1 blue cov Poey white ICG V yellow 5 ECG V CG Ld GND black ed green CHEST BACK Attachment of the ECG ICG electrodes Clean the skin at the 7 positions indicated in the figure Rub the skin firmly with an alcohol soaked tissue or if alcohol is not avail
10. e connector s and the lead wires from the electrodes The subjects can also do this themselves at home at a designated time When the device is returned to you check if the measurement has been stopped already the light flashes twice every ten seconds is still recording the light flashes every three seconds or has stopped because of empty batteries the light does not flash at all Reconnect the device to the PC and restart VU AMS 5fs short user manual AmsConfigure You can now confirm the state of the device and when needed actively stop the recording Close AmsConfigure and remove the interface cable Now remove the batteries and place the CompactFlash Card in the reader unit Move the AMS files to a designated directory Use the same name for the directory as was used as a Subject identifier see the AmsPreProcessor Help for details It is best to backup the original 5fs AMS data files as soon as possible extension 5fs discriminates the version 5fs AMS data files from the ams files generated by previous versions If the recording has been interrupted by the experimenter or by the subject multiple 5fs files with different start times will be generated Use the AmsMerge tool to concatenate the 5fs files into a single 5fs file that spans the entire recording Interruptions will be marked in this file as hold continue periods Processing VU AMS 5fs data Use the VU AMS software suite to process the VU AMS data
11. e multiple files for a single recording because the participant took a shower or because an electrode had been loose first merge all files into a single recording with AmsMerge oo DZ Ohm 1000Hz 01 ECG mY 1000Hz 02 peel the 10Hz 03 zo Ohm 10Hz 04 MYA g 0 1Hz 2 AmsPreProcessor To fu rther process the data you need to V Insert Subject ID at start of filename Export Format JAMSSfs native v A love recordings to cl amsdatah ss s S see convert the raw 5fs AMS data files to ove rerdnosto cianscsta m a format of ch oi ce usin th e Create a new subfolder for each recording AmsPreProcessor prog ai a rent I Do not overwrite existing files Close formats are supported including Biopac ASCII EDF and EDF The default is to convert the files to the native VU AMS5fs format Native format creates a different file for each of the signals which is visible in the file name e g 0603001_03131202 DZ bch 0603001_03131202 DZDT bch 0603001_03131202 ECG bch 0603001_03131202 MYA bch 0603001_03131202 SCL bch 0603001_03131202 Z0 bch 0603001_03131202 amsinv The binary amsinv AMS inventory file indicates the exact times of event button pushes and restarts 3 AmMSQRS After conversion to native AMS5fs format FIRST extract the IBI time series from the ECG signal This is done using File gt Import ECG in the AmsQRS program The AmsQRS program displays the IBI time series of the complet
12. f your subjects into a fixed number of activity categories with a fixed number of levels Labeling divides the entire measurement period into separate smaller periods For each period start and end times are given as well as a set of codes and text labels describing the state of subject during that period in terms of posture activity physical load social situation mood state work load etc To create these labeled periods you have to use the AMSGRA program in combination with the subject s diary or your observation chart video recording In the label data file all descriptive information on the subject s activity you might want to use in future analyses should be present Make sure you have labeled all periods that might be considered of interest There seems to be no urgent need to also label periods that you consider to be irrelevant or that are expected to occur in only a few subjects However we advise to always label the entire 24 hour recording For example if the labeled periods are 18 minutes on average this adds up to 78 labels per subject VU AMS 5fs short user manual The actual labels used will entirely depend on the research question Here are some examples of categories that could be considered Category Items in Category Type of activity Reading attending a meeting eating dancing PC work conversing watching TV exercising attending a musical car driving ironing etc Posture Sitting standi
13. ire connector facing up Fasten the device with the Velcro strap in the bag and gird it on with the VU AMS belt if it is more convenient you can also use your own belt Make sure the device remains in a vertical position as much as possible Marking special events A small black button is placed on top of the VU AMS device next to the two lead wire plug connectors To mark a special event push this button for about one second Pushing it will be confirmed by a short beep Stopping the measurement If you want to stop the measurement temporarily e g for taking a shower press the event button for at least 3 seconds until the green light ceases flashing Next unplug the lead wire connector from its socket and disconnect the lead wires from the electrodes The electrodes themselves are waterproof and need not be removed from the skin To restart the measurement simply follow the instructions above starting at Attachment of the lead wires Still working A small indicator light on top of the device will be flashing about once every three seconds as long as the VU AMS is recording Something is going wrong e The green light is flashing very rapidly Diagnosis The Compact Flash card is not properly installed Solution Install the Compact Flash card in the proper way e The green light is flashing rapidly Diagnosis The battery lid is not properly fastened Solution Fasten the battery lid in the proper way e You hear a d
14. ith the latest firmware installed From time to time updates may be mailed to users These need to be installed once from a CompactFlash card Detailed instructions on how to install the update will be sent with the update flashcard AmsConfigure Start and stop measurements AmsMerge merge multiple AMS files of a person AmsPreProcessor Put the various signals in separate files AmsQRS Extract the inter beat interval time IBI series from the ECG AmsRevertFormat Create AMSIMP AMSRES AMSIMP Impedance cardiography PEP scoring AMSRES Respiration signal and RSA scoring input files for VU AMS 5fs short user manual Signal recording Always use an empty CompactFlash Card with all previous files removed from the card before each new measurement Put the flash card bottom up in the VU AMS and then place two completely charged AA batteries in the battery holder Battery clips are vulnerable so do this carefully Successful placement is signaled by a triple beep tone The green indicator light flashes nervously until the battery lid is closed When the lid has been closed shake the VU AMS to make sure the battery contact is solid When the VU AMS is on standby the green light will flash twice every ten seconds This indicates the VU_AMS is ready but not recording When the VU AMS is recording the green light will flash once every three seconds Connect the VU AMS to the PC with the interface cable marked AMS Connect
15. ng lying walking etc Physical activity Social situation etc Work leisure time sleep Angry friendly happy sad anxious tired Mood state Label configuration file Heavy moderate light none Alone with significant other with friends With colleagues etc Location J At home at the office travelling restaurant When you have decided on the type of categories you want to use during labeling you need to summarize these in a label configuration file default name label cfg Example of a VU AMS label configuration file label cfg posture 10 lying 11 sitting 12 standing 13 walking 14 lying sitting 15 sitting standing 16 sitting standing walking 17 standing walking type of activity 29 desk work 30 administrative work 31 household activities 32 recreational activities 33 transportation active 34 transportation passive 35 telephone talking 36 reading internet or other recreative PC use 37 eating drinking 38 watching TV 39 sleep location 50 work 51 home 52 friends house 53 on the road 54 public building 55 physicians dentists or therapists office 56 house of family social situation 60 alone 61 with SO 62 with own children 63 with friends 64 with colleagues 65 with others 66 unknown VU AMS 5fs short user manual You can create your own label The label file really is central to the VU AMS configuration file in two ways All further
16. ouble beep the alert beep which is repeated after increasingly shorter intervals from 30 to 10 seconds Diagnosis The battery voltage is becoming low Solution Replace the batteries with fresh ones e An electrode comes off a lead wire gets disattched or the lead wire connector is pulled out by accident Solution No worries Just attach the electrode again use a spare one if necessary reattach the lead wire or plug the connector back into the socket VU AMS 5fs short user manual Appendix2 VU AMS 5fs software suite VU AMSSts software suite AmsMerge Merging Output _merged 5fs amsinv xxx bch beat AmsReverFormat Reverting Output ams Old AMS suite format AmsPreProcessor AmsORS AmsGraph converting peren peak detection msiny visualization Output Output Output almsinv beat SECG bch Use gt File gt SDZ bch Import ECG SDZDT bch P Z0 bch SSCL bch Etc AmsConfigure Configuring the VU AMSSfs device
17. processing is tailored to it By 1 use the Create configuration file looking at start and end times of the labels all option from the submenu of programs adding variables like PEP and RSA Labels in AMSGRA or will automatically attach the label information 2 use a previous made label cfg from the label data files Time stamps your An example of the text in such a activity and posture codes as well as any label configuration file is given textual label will be attached automatically to below each single value of the parameters that are being scored Although this may appear to be Make sure you copied the label cfg extreme overkill it is very convenient once file into the same directory as the you bring your final data matrix into your ams files that you want to label statistical program Use the Open AMS file command from the File menu to load a data file ams The total registration of the subject is now shown in the upper display of your screen The grey graded box between the two vertical bars indicates which part of this total registration is shown in the lower display By moving the two vertical bars with your mouse the size of the graded box and thus which part of the total registration is displayed enlarged in the lower panel can be changed a double headed arrow is displayed when this option is available You can also move the whole box to the left or the right with your mouse a hand is displaye
18. the infrared end of the interface cable to the VU AMS the electronic end of the interface important to make sure your PC has the cable goes to the serial port or correct time and date The VU AMS will the USB port of the PC verify time and date of the PC against its internal Real Time clock deviations gt 5 minutes configurable in Warnings will be flagged by AmsConfigure NOTE Before you start AmsConfigure make sure to set date and time of the PC correctly All dates and times in the AMS data files will be based on the time and date read from the PC at start up so it is Now start the AmsConfigure program from the new VUAMS5fs package note that the old VUAMS46 package also has an AMSCOM program this will no longer work AmsConfigure tries to automatically detect the VU AMS device on all available COM ports If successful the opening screen see figure below will be displayed in x U AMS device Values Serial number 31 Recording identification 0603001 Hardware revision 41 j Rees Start Device software version 5 Battery voltage 2 56 GiRGan Device state Idle Battery type nimh Time 2007 03 13 11 54 Ls ICG distance mm 200 Sampling Frequencies Timezone i f 10 5 Ohm ECG 1 kHz Thorax impedance 20 Warnings ICG dz jike gt Skin Conductance SCL 9 3 U5 g ICG z0 10 Hz v Motility 0 00g Edit config a Bel y m Compa
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