ENGLISH User's Guide
Contents
1. Battery block Sample DIAMOND Tips Tipack rack or for C5000 and C10ml a bag of tips Guarantee and quality certificate User s Guide amp Quick Guide Safety bag Tip holder reassembly tool Multichannel models only Tube of lubricant Identification tags Tip ejector extension C10 model only Accessories The following item may be supplied either in the box or separately by your Gilson distributor e AC adaptor four region types are available Europe UK USA Japan Australia The following items are not supplied in the box e Rapid charging and storage stand AC adaptor not included e PC Connection kit includes PC cable plus programming and maintenance software e Additional battery block gt Please contact your Gilson Distributor for these accessories Order with the reference numbers given in Chapter 15 MN PIPETMAN Conce RS 3 GETTING STARTED 1 2 Slide the battery block into the pipette see fig 1 Your Gilson distributor supplies an AC adaptor that is suitable for your country either in the pipette box or separately You must only use an original Gilson AC adaptor specific to this product Connect the AC adaptor to a suitable AC power supply and plug the adaptor into the pipette see figure 2 If you have a charging stand then you must first place the pipette on the stand and plug the adaptor into the stand It takes two to three hours to fully charge the battery or one hour2. 0 70 0 90 1 05 2 5 2 4 3 6 6 0 10 15 25 25 30 50 0 04 0 08 0 10 0 25 0 50 I oo I Na KOO Gilson Systematic Random error uL lt 0 013 lt 0 012 lt 0 020 lt 0 025 lt 0 10 lt 0 10 lt 0 12 lt 0 15 lt 0 16 lt 0 20 lt 0 23 lt 0 30 lt 0 4 lt 0 4 lt 0 8 1 2 lt 2 lt 4 lt lt 4 lt 8 lt 12 lt 0 02 lt 0 04 lt 0 06 lt 0 14 lt 0 20 lt 0 25 lt 0 18 lt 0 38 lt 0 60 The data conform to ISO 8655 2 Standard ISO 8655 Systematic Random error uL error uL 0 120 lt 0 080 0 120 lt 0 080 0 120 lt 0 080 0 120 lt 0 080 0 80 lt 0 30 0 80 lt 0 30 0 80 lt 0 30 0 80 lt 0 30 4 00 lt 1 50 4 00 lt 1 50 4 00 lt 1 50 4 00 lt 1 50 16 0 lt 6 0 16 0 lt 6 0 16 0 lt 6 0 16 0 lt 6 0 40 lt 15 40 lt 15 40 lt 15 60 lt 30 60 lt 30 60 lt 30 0 24 lt 0 16 0 24 lt 0 16 0 24 lt 0 16 1 60 lt 0 60 1 60 lt 0 60 1 60 lt 0 60 8 0 lt 3 0 8 0 3 0 8 0 3 0 LS Recommended tip for establishing maximum permissible errors ISO 8655 requirement A large range of suitable Gilson DIAMOND tip is available Please contact your local Gilson distributor for further information a7 3 MN PIPETMAN Conce RS nina nnn OO OOOO OOOO OOOO OOOO OOOO OOOO OOOO OOOO OOOO OO
3. Figure 24 Reassembling the Piston Assemblies Tip holders and Plastic Ejector cover A Tip holders 1 Usealubricated piston to transfer a small quantity of lubricant to the O ring Seal holder 2 With reference to the figure 26 place the components on the Spring reassembly tool in the following order locking clip tip holder spring seal holder O ring take care that the spring is the correct way up and the flat surface of seals rings and washers faces the spring 3 Insert the components into the tip holder with the aid of the reassembly tool 4 Push on the locking clip until it snaps into place Seal O ring Locking clip Figure 26 is MN PIPETMAN Conce RS B Piston Assemblies 1 Lubricate the piston as described in the section below 2 Gently slip a lubricated piston into the tip holder with a slight twisting motion Avoid using too much force as this could dislodge the O ring C Closing up 1 Refit each tip holder piston assembly so that the top of the piston and the tip holder snap into the upper and lower rakes respectively To facilitate fitting a piston assembly to the upper rake you should incline the piston assembly and compress the piston spring against the upper rake before pushing the assembly into place 2 Close the piston tray cover after checking that all assemblies are correctly installed 3 Refit the wire clips wire guides for 12 channel models 4 Slide the ejector cover back
4. MN PIPETMAN Conce CS ENGLISH Other languages available on our website www gilson com MN PIPETMAN Conce RS 10 INTRODUCTION Accessories GETTING STARTED Battery charging Setting up Initializing Standby Screen DESCRIPTION Pipette Keypad Screen LCD Tip ejector Extension OPERATION Switching On Thumbwheel Start Button Speed Buttons Direction Button Mode Buttons Reset Button and Connectors Tip Ejector Button PIPETTING MODES Overview Selecting Aborting the Active Mode Mode Description AUTO AUTO MIX MANUAL REPETITIVE REVERSE Programming Modes DILUTE DILUTE AND MIX MULTIPLE ASPIRATES DISTRIBUTION WITH TIMER GILSON DIAMOND TIPS PIPETTING TECHNIQUES Guidelines for Good Pipetting Aspirate and Dispense GLP FEATURES General Cycle counters Alarms SYSTEM MENU Configuration Beeper Screen contrast Volume limit PARTS CHECK LIST AND ACCESSORIES oooO o ooo oso al oooO aos aida oo 11 12 13 14 15 16 17 18 SYSTEM MENU Calibration Standard Readjustment Quick Adjustment Factory Settings Maintenance Disassembly Service Info Service Settings Maintenance Alarm Pipette Info Personalization USER MAINTENANCE Maintenance Warnings Maintenance Operations Maintenance Operations for Single channel Tip holder and Tip ejector Changing the Tip ejector Changing the Tip holder Changing the O ring or seal for C10 Servicing the Piston Removi
5. Nr Nr To aspirate keep the START button pressed You can stop by releasing the START button and restart by pressing it again You can aspirate until Vmax or the nominal volume is reached Press the DIRECTION button to change the direction in which the piston moves from up to down see direction arrow on the dis play You can change direction at any time during an aspirate or a dispense cycle AUTO AUTO MIX MANUAL REPETITIVE REVERSE PIPETMAN Concept Indicates the volume Indicates the aspirated or remaining volume limit to dispense MANUAL 100 uL a 0 pL Speed 3 M PIPETMAN Cn Indicates piston direction in real time START button ORelease START Start Purge Dispense keep the START button pressed At any time you can stop by releasing the START button and restart by pressing it again When the volume reaches 0 0 uL dispensing stops Purge click on START to purge and reset the piston BLOW OUT appears on the screen until the piston has reset to zero IS The volume of liquid present in the tip is permanently displayed in real time As a consequence you can use the MANUAL mode to measure unknown volumes However take care to reset the selected volume Vmax to the nominal volume e g 1200 uL for a C1200 REPETITIVE a Description This mode distributes a number N of equal aliquots You AUTO MIX supply the aliquot volume AV and the pipette automatical
6. F2070701 F2070719 F3070902 F161280 F161240 Reference F30750 F3075502 F3071107 F30701 F30702 F30703 F30704 C10ml F3071165 F3071110 F3071103 F2070859 F3071752 F2070801 F2070819 F3070902 F161280 F161240 IS 1 Theairtightness kit include the following items Locking clip Spring Seal holder Seal O ring 2 The tip holder assembly 3 includes the above Figure 28 items fitted into the tip holder 33 MN PIPETMAN Conce RS Replacement Parts for PIPETMAN Concept Multichannel Item 8x10 8x100 8x300 12x10 12x100 12x300 Battery block O F3071165 F3071165 F3071165 F30 1169 F3071165 F3071165 Adjustable hook F3071110 F3071110 F3071110 F3071110 F3071110 F3071110 Personal window O F3071103 F3071103 F3071103 F3071103 F3071103 F3071103 Screws and wire clips kit 2 F2073261 F2073261 F2073261 F2073361 F2073361 F2073361 Tip holder assembly G FI T system 3 F3073255 F3073355 F307326306 F3073255 F3073355 F307326306 Ejector cover F307325051 F307335051 F2073259 F307425051 F307435051 F2073359 Piston assembly 5 F3073254 F3073354 F3073454 F3073204 F3073354 F3073454 Air tightness kit F3073360 F2073265 F3073360 F2073265 Reassembly tool F3073201 F3073301 F2073201 F3073201 F3073301 F2073201 Lubricant tube 3 5 g F3070902 F3070902 F3070902 F3070902 F3070902 1 6 SAFETY PRECAUTIONS AND LIMITATIONS OF USE For safety reasons it is important to observe the following instructions 1 Battery and e
7. and if required autoclave the lower part of the tip holder 4 If required lubricate the piston see How to Lubricate the Piston and fit a new O ring 5 Screw the two parts together making sure that the two parts are fully tightened by hand using latex gloves 6 Refit the tip ejector Changing the O ring or seal for C10 The O ring is contained by the two halves of the tip holder if worn or damaged in any way it must be replaced To access the O ring remove the tip ejector and unscrew the lower part of the tip holder if the O ring is not immediately visible on the piston select the AUTO mode then set the volume to the nominal and press the ACTION button You should now be able to remove the O ring from the piston Sometimes the O ring may be found in the recess at the top end of the lower part of the tip holder 24 If required lubricate the piston see How to Lubricate the Piston then fit a new O ring by sliding it onto the piston Reassemble the pipette Servicing the Piston A You may remove the piston assembly to clean lubricate or change the piston The specifications of the pipette must be checked after changing the piston Removing the Piston A Use the stand by procedure Disassembly see Chapter 10 Maintenance 1 Selectthe Disassembly option inthe System menu System menu gt Maintenance gt Disassembly 2 Remove the tip ejector and optionally the lower part of the tip
8. autoclavable Only the following parts may be autoclaved in dividually tip ejector tip holder both parts connecting nut piston including holder return spring spring guide O ring warning can be damaged when autoclaved and airtightness kit Multichannel models 1 Clean the parts to be autoclaved especially the tip holder 2 3 4 5 Put the parts in an autoclaving sack Autoclave at 0 1 MPa at 121 C for 20 minutes Check that the parts are dry before reassembling the pipette Set the pipette aside to stabilize at room temperature 290 MN PIPETMAN Conce RS A The specifications of the pipette must be checked after autoclaving Chemical Decontamination You may choose to decontaminate your pipette chemically in accordance with your own procedures Whatever decontaminant you use check that it is compatible with the plastics used in the construction of the pipette see previous page e Parts that may not be immersed 1 Wipe the handle of the pipette with a soft cloth or lint free tissue impregnated with the chosen decontaminant 2 Wipe the handle of the pipette with a soft cloth or lint free tissue impregnated with distilled water e Parts that may be immersed The following components only can be immersed in a decontaminant solution tip ejector tip holder both parts connecting nut piston including holder return spring and spring guide 1 Disassemble the pipette as described in Main
9. for 80 charge We strongly recommend you fully charge the battery before using the pipette Battery Charging When charging the pipette you see the following screen Get a second battery block that way one can be charged while the pipette is in use you can plug the battery block into the charging stand Setting up 1 Contacts for charging the battery from the stand Power Supply Figure 2 Volume range 20 300 uL Pipetman Concept Charging V2 10 PIPETMAN C cep Version number of the firmware Set the position of the adjustable hook to the position of maximum comfort for you size of hand see figure 3 Loosen the retaining screw do not undo completely slide the hook into the most comfortable position and then retighten the screw Fit Gilson DIAMOND Tips appropriate for the model of PIPETMAN Concept that you are using see Chapter 7 A You must always fit a tip before using any pipette Rotate the tip adjustment force regulator until you reach the optimum force for you and the type of tip being used see figure 3 Optionally you may personalize the name of your pipette see Chapter 10 Initializing Press any button to activate the pipette You then see the start up screen Select your mode see Chapter 6 and enjoy using your PIPETMAN Concept IS To get used to the pipette we recommend you select the AUTO mode Standby Screen PIPETMAN Concept goes to stand by af
10. obtained using mode AUTO speed 5 These figures are only guaranteed using Genuine Gilson DIAMOND Tips Each pipette is inspected and validated by qualified technicians according to the Gilson Quality System Gilson declares that its manufactured pipettes comply with the requirements of the ISO 8655 Standard by type testing The adjustment is carried out under strictly defined and monitored conditions ISO 8655 6 Basis of adjustment Ex Reference temperature 20 C Relative humidity 50 Barometric pressure 101 kPa Use of distilled water grade 3 ISO 3696 Ten measurements for each test volume which are Nominal Volume 50 of Nominal Volume and 10 of Nominal Volume 36 1 8 GILSON MAXIMUM PERMISSIBLE ERRORS Tested mode AUTO Speed 5 Model Color coding Reference Range C10 Red F31012 05 10 uL C100 Peach F31013 5 100 pL C300 Green F31014 20 300uL C1200 Blue F31015 100 1200pL C5000 Purple F31016 500 5000uL C10ml Light blue F31017 1 10mL 8x10 12x10 Red F31032 F31042 0 5 TOUL 8x100 12x100 Peach F31033 F31043 OO 8x300 12x300 Green F31034 F31044 20 300uL DIAMOND Tips D10 DL10 D200 D300 D1200 D5000 D10ml DL10 D200 D300 Volume uL 0 5 1 9 10 10 10 50 100 30 150 300 error uL 0 040 0 025 0 060 0 080 0 35 0 30 0 38 0 40 0 80
11. on the thumbwheel rotate the thumbwheel to select a volume default nominal then click again to confirm the value gt Configuration Calibration Maintenance Personalization Quit PIPETMAN Concep LS asterisk means that the system menu is locked gt Configuration Calibration Maintenance Personalization Quit PIPETMAN Concept Y gt Beeper OFF Contrast 3 Volume limit 300 0 Quit PIPETMAN Concep To return to the currently active screen select Quit and click to confirm MN PIPETMAN Conce RS Calibration As part of your quality system you may have standard operating procedures for pipette cali bration In accordance with ISO 8655 Gilson recommends a gravimetric procedure for pipette calibration Please refer to Verification Procedure for Accuracy and Precision reference LT802292 which you can download from the Gilson website This gravimetric method is used to establish the mean mass of a given volume of water tak ing into account evaporation losses where necessary After converting the mean mass to a volume using the Z factor you enter the measured volumes s into the pipettes memory and the software readjusts the pipette accordingly PIPETMAN Concept allows the user to adjust the pipette quickly with maximum accuracy and with the flexibility to use one three or four calibration points see below The calibration menu allows you to access the following items Standard O gt
12. over the piston tray and reattach using the two screws How to Lubricate the Piston all models except C10 and Multichannel x10 Use only Gilson lubricant ref F3070902 as supplied Squeeze a small quantity from the tube onto a clean nonabrasive cloth Use the cloth to transfer the lubricant to the piston Ensure that the piston is evenly lubricated and that you wipe away any excess remember only a fine film of lubricant is required over the entire piston 1 2 CLEANING AND DECONTAMINATION PIPETMAN Concept is designed so that the parts normally in contact with liquid contaminants can easily be cleaned and decontaminated As part of your quality system you may have procedures for pipette decontamination We recommend that you refer to Decontamination Procedures for Gilson Pipettes reference LT802288 which you can download from Gilson s website A Liquid must not enter the handle of the pipette If you use chemical solutions for decontamination or detergents for cleaning other than specified below you should check with your supplier that the solution or detergent used does not attack any of the following materials stainless steel POM Polyoxymethylene PVDF Polyvinylidene fluoride PC Polycarbonate and PBT Polybutylene Terephthalate 290 Cleaning The pipette must be cleaned as described below before it is decontaminated You may use a simple soap solution for cleaning PIPETMAN Concept or any of the soluti
13. ri peut i i ibrati ui ju Readjustment Quick Re adjustment Maintenance Factory settings or Factory setting Personalization Quit Quit ES Following readjustment by the user Fn FY BD ote is ieplayedontre WPIPEIMANC PIPETMAN Cccp active screen to indicate the num ber of times the pipette has been readjusted since manufacture The value of n is increased by 1 when you use Quit to leave the screen after a modification or confirmation of the values You select the operation that you wish to perform using the thumbwheel and then click the thumbwheel to access the corresponding parameters After this select the volume that you wish to enter click the thumbwheel turn the thumbwheel until you see the measured volume and click to confirm the value Continue this process for the other volumes if any then select Quit and click to confirm Standard Readjustment This menu allows you to readjust gt Std readjustment pr Measured volume Quick adjustment gt 10 30 24 the pipette using 3 calibration points Factory serines 50 301 05 10 50 and 100 of nominal Quit dl ui volume nominal according the recommandation ofISO8655 These PIPETMAN Concept PIPETMAN Concep three values are stored inthe firmware Ms Quick Adjustment Measured volume ES Quick adjustment volume This menu allows you to adjust the gt Quick adjustment gt Target 20 00 i Factory settings Measured 20 57 pipette using 1 cal
14. N Conce RS IS The seal for C10 is fragile and can only be used once So after unscrewing the lower part of the tip holder you must fit a new seal 9 Quit the Disassembly screen Quit gt Yes Maintenance Operations for Multichannel models Removing and reassembling the Head A Use the stand by procedure Disassembly see Figure 18 Chapter 10 Maintenance A 1 Select the Disassembly option in the System menu System menu gt Maintenance gt Disassembly 2 Keep the tip ejector button depressed and grip the ejector clip 3 Unlock the ejector clip and separate it from the activating rod figure 18 iN Unscrew the connecting nut Extract the head After reassembling the head quit the Disassembly screen Quit gt Yes al O Removing the Plastic Cover Piston Assemblies and Tip holders 1 Remove the two screws which are located at either side of the head us ing a miniature screwdriver figure 19 2 Gently pull the ejector cover away from the head figure 20 3 Removethe wire clips wire guides for 12 channels models from the piston tray cover figure 21 4 Lay the pipette flat and push awards with your thumbs on the catches at either side of the piston tray cover figure 22 The cover will flip upwards to reveal piston and tip holder assemblies 5 Lift the tip holder upwards away from the lower rake INS thenpushthetip holderback Pisto
15. OO OOOO OOOO OOOO OOO EC DECLARATION OF CONFORMITY The company GILSON S A S 19 avenue des Entrepreneurs BP 145 95400 Villiers le Bel France Tel 33 0 1 34 29 50 00 Fax 33 0 134 29 50 20 Hereby certifies on its sole responsibility that the products listed below PIPETMAN Concept C10 C100 C300 C1200 C5000 C10ml 8x10 8x100 8x300 12x10 12x100x 12x300 98 79 EC comply with the requir ees of In Vitro Diagnostic Medical Devices the following European Directives 2004 108 EC Electromagnetic Compatibility EMC 2006 95 EC Low Voltage Directive LVD Annex III self declared Villiers le Bel August 5th 2009 Atika El Sayed Herv Ledorze General Manager Quality Manager PGS ona OOOOOOOOOOCOOOOOOCOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOWODOD QOOOOOOOOOOOOOOOOOOOOOOPOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOMOOOOOOOOOOOOOOOOOHE OODD DODDO OOOO ODODO OOOO OOOO OOOO OOO OOOO OOO OCO OOOO OOOO OOOO OOO OOO OOOO OOOO OOOO OCOC OOOO OOOO OOOO OOO OOOO OOO OOO OOOO OOOO OOOO OOOO OOOO OO OOOO OOO OOOO OO OOOO OOO OOOO OOOO OOO Q 38 Nores 39 LT801489 D 02009 Gilson SAS All rights reserved November 2009 English Printed in France Specifications subject to change without notifications errors omitted www gilson com sales gilson com service gilson com training gilson com Gi
16. QUES Guidelines for Good Pipetting 1 Make sure that you fit new tips 2 Each new tip s should be pre rinsed with the liquid to be pipetted 3 When aspirating keep the tip s at a constant depth below the surface of the liquid refer to Table 1 4 Change the tip s before aspirating a different liquid sample or reagent 5 Change the tip s if a droplet remains at the end of the tip s from the previous pipetting operation 6 Liquid should never enter the tip holder To prevent this Never turn the pipette upside down Never lay the pipette on its side when there is liquid in the tip s Refer to the specific mode for details of the functionality 7 The use of the Gilson Charging Stand see Chapter 15 is recommended to store your PIPETMAN Concept pipette in the vertical position 8 When pipetting liquids with temperatures different to the ambient temperature pre rinse the tip s several times before use in order to reach an equilibrium between the temperatures of the liquid and the pipette s air cushion 9 For volatile liquids you should saturate the aircushion of your pipette by aspirating and dispensing the liquid repeatedly before aspirating the sample 16 10 After pipetting acids or other corrosive liquids that emit vapors clean the pipette as de scribed in Chapter 12 11 The pipette can be used between 4 C and 40 C but the specifications may vary 12 Do not pipette liquids having temper
17. T button to complete the current mixing cycle v Purge click on START to purge and reset the piston BLOW OUT appears on the screen until the piston has reset to zero ES During the normal aspirate and dispense phase the word AUTO appears the volume displayed is the sample volume During the mixing phase the word MIX appears the volume displayed is the mix volume In this mode the user has access to a cycle counter which is incremented after each pipetting cycle It counts up to 999 before starting again at zero The counter is reset to zero each time the volume is changed MN PIPETMAN Conce RS MANUAL a Description This mode simulates the way in which a manual pipette is operated The user can stop and start the aspirate and dispense phases at will This enables you to load a gel or measure a sample volume You can also set a volume limit before aspirating a liquid phase b Protocol i Set the volume limit optional click on the thumb wheel than turn it until you see the selected volume limit Vmax displayed on the screen then press the thumbwheel to lock the volume you cannot dispense until this is done If you do not set a vol ume the default volume is the nominal volume of the pipette li Set the speed optional press the keys to in crease or reduce the speed default is 5 The speed can be changed before or during a pipetting cycle c Functionality i
18. arts if required To help you to keep up a regular schedule and in the interests of GLP you can configure your pipette to display an alarm before a service is due refer to Chapter 10 Setting maintenance alarm to Or results in the following messages Maintenance Warnings e Date of nextservice warning about 1 week before and if overdue e If you exceed a service interval that was set relative to the number of pipetting cycles the system flashes the following message place of the volume indicator Push any button and the following is displayed Select Maintenance to go to the system menu or Remind me later to delay by one day e If you exceed a service interval that was set relative to a time limit the system flashes the following message place of the volume indicator Select Maintenance to go to the system menu or Remind me later to delay by one day Maintenance Operations You may perform the following operations yourself Cycles 20000 gt Weeks 36 Date 18 JAN 07 Alarm ON Quit PIPETMAN Concept SERVICE PIPETTE NOW Time limit passed gt Maintenance Remind me later PIPETMAN Concept Clean or autoclave the parts specified under Cleaning and Decontamination Chapter 12 Change the parts specified under Replacement Parts Chapter 15 Lubricate the piston Change the battery lt gt Please contact your Gilson distributor if you require the
19. atures above 70 C or below 4 C Extreme temperatures can affect accuracy and precision Aspirate and Dispense 1 Fitnew Gilson DIAMOND Tip s for the best results see Specifications Chapter 17 Push the tip holder into the tip using a slight twisting motion to ensure a firm and airtight seal 2 Pre rinse the tip s Pre rinsing consists of aspirating the first volume of liquid and then dispensing it back into the same vessel or to waste Subsequent volumes that you pipette will have levels of accuracy and precision within specifications Some liquids e g protein containing solutions and organic solvents can leave a film of liquid on the inside the wall of the tip pre rinsing the tip minimizes any errors that may be related to this phenomenon 3 Hold the pipette vertically and immerse the ion Depth Wait Ti ld Geo et Table 1 Immersion Depth and Wait Time Press START to aspirate the set volume of Model Immersion Depth Wait Time liquid Wait a few seconds time depends mm Seconds on model see Table 1 then withdraw the 0 8x10 12x10 1 1 pipette tip from the liquid C100 8x100 12x100 2 4 1 You may wipe any droplets away from the C300 8x300 12x300 2 4 1 outside of the tip s using a medical wipe ae q pe however if you do so take care to avoid ci0mi 57 4 5 touching the tip s orifice 4 Place the end of the tip s against the inside wall of the recipient vessel at an angle of 10 to 40 Press th
20. e START button Wait for at least a few seconds before releasing the START button to expel any residual liquid from the tip s While removing the pipette draw the tip along the inside surface of the vessel 5 Eject the tip s by pressing firmly on the tip ejector button MN PIPETMAN Conce RS 9 GLP FEATURES Your PIPETMAN Concept is fully compliant to IS08655 standard and is CE marked for IVD and EMC directives PIPETMAN Concept enables error free manipulations and includes the following GLP features General Lockable volume Name of model is marked on the color coded START button Volume range is displayed on the screen and on the ejector cover Multichannel models Serial number is engraved on the handle of the pipette and encoded in the firmware Bar code on the box and with the certificate can be transferred Personalization by nametag for marking application or user name and by encoding in the firmware The name appears on the screen when the pipette goes to standby after 3 minutes of inactivity System menu can be locked from a PC thanks to the Utility Software see Chapter 10 Cycle counters From last volume setting counts the number of cycles in the current run 0 to 999 Appears on the main screen From manufacture and since the last service see Chapter 10 Indicators R1 R2 R3 Rx each time pipette is readjusted see Chapters 10 amp 17 Maintenance intervals can be specifi
21. e after you release the START button again excess volume LS the needed volume plus an automatically selected gt Also you can abort the cycle and purge by pressing one of the Mode buttons in every modes The REVERSE mode may replaced by a pre programmed mode uploaded from your computer see below Programming Modes You may replace REVERSE with your own custom built mode which you have programmed on a Personal Computer PC with the PIPETMAN Concept Utility Software You have total flexibility and an almost unlimited number of combinations are possible see program guide To operate in the program mode you need a PC and a kit reference F30755 containing the necessary software and connectors The kit is available from your usual Gilson distributor see Chapter 15 Typically you can do the following and combine protocol tasks at will DILUTE Aspirate volumes V1 and V2 with an air gap between the two volumes then distribute the ensemble DILUTE AND MIX Aspirate volumes V1 and V2 with an air gap between the two volumes mix as above then distribute the mixture MULTIPLE ASPIRATES Aspirate N times a volume and then distribute the ensemble DISTRIBUTION WITH TIMER Distribute multiple aliquots with a user defined time interval between each F GILSON DIAMOND TIPS Gilson DIAMOND Tips are made to the highest specifications strict quality control is maintained throughout the manufacturing process F
22. ed by weeks or number of cycles elapsed These and other parameters may be locked by the using the PIPETMAN Concept Utility Software Alarms Low battery warning flashes in the place of the volume indicator Push any button and the message disappears this gives a few minutes of use enabling you to finish the current manipulation and then to recharge the battery or replace with another charged battery Volume out of specifications warning for Program mode Service is overdue warnings see Chapter 10 Beeper can be set On or Off 1 0 SYSTEM MENU Press both Mode buttons at the same time to accede to the System Menu This menu lets you choose between 4 sub menus Configuration Calibration Maintenance and Personalization Select a menu by rotating the thumbwheel then click on the required item to enter the sub menu select an item from the sub menu in the same way You can modify the parameters associated with each one provided that the sub menu was not locked by computer based software using the PC Connection Kit see Chapter 15 Configuration This menu allows you to configure the following items Beeper Click on the thumbwheel to set the beeper ON or OFF the beeper will not operate except in the case of an error or f set in Program mode Screen contrast Click on the thumbwheel rotate the thumbwheel to select a value from 1 to 5 then click again to confirm the value Volume limit Click
23. holder If you remove the lower part take care to remove the piston seal as described in Changing the O ring 3 Unscrew the connecting nut turn by hand coun terclockwise figure 14 Tip holder lower Figure 15 4 Pull on the spring guide to remove the piston as Connecting sembly from the handle of the pipette separate nut the parts figure 15 For C5000 and C10 ml the Le Spring guide connecting nut is combined with the upper part of sil the tip holder see Spare Parts de 5 Clean and autoclave if required the piston and LE holder together with any other parts that may need Piston Spring tapered to be treated in the same way see Cleaning and Piston holder Decontamination 6 Lubricate the piston except C10 see at the end of chapter 7 Reassemble the piston spring and spring guide then carefully insert the assembly into the handle of the pipette figure 16 The spring guide should hold the piston assembly inside the handle of the pipette Take care to position the small diameter end of the spring as shown innermost and that the flanged end of the spring guide is outermost 8 Reassemble the upper part of the tip holder and the connecting nut and then refit to the handle of the pi pette by rotating the connecting nut clockwise until it is finger tight figure 17 Fitthe seal and reassemble the lower part of the tip holder Refit the tip ejector 95 MN PIPETMA
24. ibration point Quit Quit Effectively by adding a fourth point to the standard calibration values By PIPETMAN C pt PIPETMAN C pt once once default the value stored in the firmware is zero in which case itis not taking into account as a fourth calibration point LS f the value entered for Calibration volume is the same as one of the values used for the Standard re adjustment then the Measured volume of Quick readjustment will replace the Measured volume of Standard readjustment when calculating the correction to be used Factory Settings This option allows you to reset the volumes to the three factory calibration values Std Adjust ment which are permanently stored in the pipette s firmware mes If you choose this option you will erase the fourth calibration point from the Quick Readjustment procedure Maintenance This menu allows you to check and set parameters relating to pipette maintenance Select a sub no menu by rotating the thumbwheel then click on gt Maintenance the required item to enter the sub menu select oo A ul an item from the sub menu in the same way Routine maintenance procedures are described PIPETMAN Concept in the next Chapter Disassembly This option allows yon to gt Disassembly gt It is now safe to disassemble the lower part Service info disassemble the safely protection of the Service settings Lower part i Pipette info piston and the motor If you Quit gt Quit select Disasse
25. ich may be set from 1 to 5 of a pipetting cycle to increase the speed to decrease the speed LS The speed can be changed before or during a pipetting cycle Direction Button O Press this button to change the direction in which the piston is moving The arrow on the screen will change accordingly ES This button is only active in the MANUAL mode see Chapter 6 Mode Buttons There are two buttons located at either side of the pipette one each for left or right handed operators Press either of these buttons to display the mode menu on the screen see Chapter 6 Pressing both atthe same time will switch the pipette to System menu see Chapter 10 PC Connector Serial Port Interface Reset Button and Connectors figure 7 The reset button is recessed at the back of the pipette between the power supply and the PC connector Insert a small plastic Reset Button i r reinitialize the pi Power Suppl probe or a tip and press to reinitialize the pipette Figure 7 pply Tip Ejector Button figure 8 Tip ejector E Button This is a mechanical button that can be set for left or right YU handed users Figure 8 6 PIPETTING MODES Overview PIPETMAN Concept has five pre programmed modes The AUTO mode is the recommended for the new user to become familiar with the operation of PIPETMAN Concept Selecting Press either of the Mode buttons The Mode menu shows on the LCD screen The active m
26. igure 9 DIAMOND Tips are used to calibrate PIPETMAN Concept therefore i ID Number for optimum performance you are strongly advised to use Gilson s DIAMOND Tips with your PIPETMAN Concept DIAMOND Tips have the Gilson logo engraved on their collar ensuring that you have a genuine Gilson product To ensure accuracy and precision Gilson s Quality Assurance System focuses on the following critical parameters e DIAMOND Tips are made from pure polypropylene virgin metal free to avoid the possibility of contamination They are available sterilized and with filters MN PIPETMAN Conce RS Gilson s sterilized DIAMOND Tips are certified free of detectable RNases DNases DNA RNA and proteases Optimized shape revised collar for optimum sealing thin walls and fine point making them easier to mount more flexible with no vortexing and improved precision DIAMOND Tips are free from defects especially at the orifice All surfaces are smooth and hydrophobic thereby avoiding the excessive retention of liquids that causes poor accuracy and a lack of precision Mold and cavity references are marked on the collar ensuring the traceability for quality assurance purposes batch numbers appear on all packages bags and boxes They form an air tight seal with the tip holder preventing the leaks that cause poor accuracy and a lack of precision They may be autoclaved at 121 C for 20 minutes at 0 1 MPa 8 PIPETTING TECHNI
27. ion with the other b Gently twist the extension either direction and pull it away from the pipette 5 OPERATION Switching On figure 6 Press any button to activate the pipette After pressing a button if nothing shows on the screen check that the battery is charged see Chapter 3 Thumbwheel There are two ways of using the thumbwheel by rotating in either direction or by pressing inward sharply clicking For example to set the volume 1 Click the thumbwheel to unlock the volume 2 Rotate the thumbwheel to set the volume 3 Click the thumbwheel to lock the volume Figure 5 Thumbwheel Mode Direction Start Mode Button Button Button Button Figure 6 MN PIPETMAN Conce RS The thumbwheel is also used to select parameters and to choose from the System menu How ever it always operates in the same manner click then turn to select and click again to confirm your choice These options are explained in the following text at the appropriate point LES When setting the volume use a sharp motion on the thumbwheel to move rapidly through the volume range or use a slow and deliberate motion to fine tune the volume Start Button This button is the equivalent of the plunger on a manual pipette However very little force is needed to operate it A simple click is all that is needed to aspirate and dispense or to purge the pipette Speed Buttons These buttons are used to change the speed wh
28. ir assistance in the form of training or if you would like to enter into a service contract with them A Go to Maintenance gt Disassembly and confirm to disassemble the lower part safely see page 21 08 MN PIPETMAN Conce RS Maintenance Operations for Single channel models Tip holder and Tip ejector These parts must be changed if they are accidentally damaged or attacked chemically You should also remove these parts for cleaning or decontamination purposes Changing the Tip ejector DN Figure 10 1 Keep the tip ejector button depressed and grip the top of the tip ejector with the other hand 2 Gently rotate the tip ejector counterclockwise and separate its connector from the activating rod figure 10 3 Pull the tip ejector away from the handle of the pipette 4 Clean or autoclave the tip ejector and refit it or a new one by reversing the procedure Changing the Tip holder lower part After removing the tip ejector you may remove the lower part of the tip holder which is more likely to become contaminated or damaged than the upper part Removal of the lower part is shown below for the upper part refer to Removing the Piston special precautions are necessary 1 Gently rotate the lower part of the tip holder counterclock Figure 11 wise to unscrew it from the upper part figure 11 2 Separate the parts and remove the O ring see Changing the O ring figures 12 and 13 3 Clean
29. lectrical specifications We strongly recommend you fully charge the battery before using the pipette Your Gilson distributor supplies an AC adaptor that is suitable for your country either in the pipette box or separately You must only use an original Gilson AC adaptor specific to this product Charge the battery in the pipette using the AC adapter or the charging stand or in the charging stand slot for additional battery Use AC adaptor and charging stand indoors When handling the battery do not allow the battery to come into contact with any metallic surfaces Do not short circuit Dispose of used batteries in accordance with legal regulations Batteries may not be dis posed of with household waste and may explode if disposed in fire e Li ion battery pack 1 Ah 3 6 V Charging time approx 2 hours for a fully discharged battery 80 in one hour Symbol means Caution risk of danger e AC adaptor may be not included see above Input voltage country specific 100 240 V 50 60 Hz 0 5A max Output voltage 5 V DC 3 5A 17 5W max Sl 2 Do not use PIPETMAN Concept in a potentially explosive environment or with potentially explosive chemicals 3 When pipetting infectious radioactive toxic and other hazardous solutions please observe all the safety precautions e g wear protective clothing goggles and gloves and regulations appropriate for your country 4 Do not allow the liquid to enter the body of the pi
30. lson Inc World Headquarters 3000 Parmenter Street PO Box 620027 Middleton WI 53562 0027 USA Tel 1 800 445 7661 or 1 608 836 1551 Fax 1 608 831 4451 Gilson S A S fr LU GILSO 19 avenue des Entrepreneurs BP 145 F 95400 Villiers le Bel France Tel 33 1 34 29 50 00 Fax 33 1 34 29 50 20
31. ly oe calculates the number from the nominal volume NV of the REVERSE pipette as follows N NV AV e g 120 uL x 10 for C1200 an PIPETMAN Concep i Set the aliquot volume click the thumbwheel the default volume flashes To change the volume turn the thumbwheel until you see the required volume displayed on the screen AS ii Then press the thumbwheel to lock the volume and cal H 120 x10 culate the number of aliquots The number of aliquots flashes You can change the num ber of aliquots using the thumbwheel or simply press the PIPETMAN Concept thumbwheel to accept the number displayed Set the speed optional press the keys to increase or reduce the speed default is 5 The speed can be changed before or during a pipetting cycle c Functionality i Immersethetipinthe liquid V extra vol to be aspirated ii To aspirate click press and release the START button and wait until the motor stops check piston position indicator lil To dispense click press and release the START button N times to sequen tially dispense the aliquots START button Optional STOP 3 Start Purge The number of aliquots left Nr o ES the needed volume plus an automatically selected excess to dispense is displayed on volume the screen iv iv BLOW OUT appears on the screen when no aliquots are left to dispense Click on START to purge and reset the piston However if you keep the button pres
32. mbly the following screen appears PIPETMAN Concep PIPETMAN Concep To close the Disassembly menu the only possibility is to choose Quit If you select Quit a confirmation message appears If you select No the display will return to the Disassembly menu If you select Yes you must first ensure that the pipette has been correctly reassembled see Chapter 11 The software then resets the piston and the display returns to the last mode used is MN PIPETMAN Conce RS Service Info Disassembly gt Last Service This screen is read only you cannot gt Service info gt Cycles 20537 Service settings Date 18 JAN 07 modify any of the items which are Pipette info the number of cycles and the date Quit Quit since the pipette was serviced PIPETMAN Concep PIPETMAN Concep Service Settings Select an item by rotating the thumbwheel then click on the TTT required item to select it rotate the thumbwheel to selecta value Service info c gt Service settings e g Weeks and then click on the thumbwheel to confirm o You can change four variables Cycles to next service bs Weeks to next service Date of the service and Alarm PIPETMAN G ncept see below When it comes to setting the date you have three fields to modify one after the other day month year dd mm yy in the same way as the other parameters mentioned above LS The 3 letter
33. ng the Piston Maintenance Operations for Multichannel Removing and reassembling the Head Removing the Plastic Cover Servicing the Ejector cover Reassembling the Piston Assemblies How to Lubricate the Piston except for C10 CLEANING AND DECONTAMINATION Cleaning External Internal Decontamination Autoclaving Chemical Decontamination LEAK TEST C10 to C300 Single and Multichannel C1200 to C10ml TROUBLESHOOTING Accessories Replacement Parts for Concept Single channel Replacement Parts for Concept Multichannel SAFETY PRECAUTIONS SPECIFICATIONS GILSON MAX PERMISSIBLE ERRORS EC DECLARATION OF CONFORMITY NOTES CONTINUED ACCESSORIES amp REPLACEMENT PARTS 1 INTRODUCTION Thank you for choosing Gilson PIPETMAN Concept the latest and most user friendly of motorized pipettes It is another quality product from Gilson which is fully IS08655 compliant CE labeled conforms to the EC directives on in vitro diagnostic medical devices electromag netic compatibility and safety To ensure the best performance from your Gilson pipette you should always use Gilson DIAMOND Tips in accordance with 1508655 to complete your pipetting system because DIAMOND Tips were used to establish the specifications cited in Chapter 17 Please refer to Chapter 16 for Safety precautions and limitations of use 2 PARTS CHECK LIST AND ACCESSORIES The following items are supplied in the box PIPETMAN Concept pipette
34. ns wire guides for Rake upper 12 channel models a along the axis of the piston Tip holders Rake lower Wire clips Wire guides e Now pull the piston away from the upper rake and lift it and the tip holder out of the tray taking care not to lose or damage the piston spring 6 Gently pull the piston assembly out of the tip holder Servicing the Ejector cover Piston Assemblies and Tip holders all models except Multichannel x10 Tip holder 8x300 12x300 The tip holders and piston assemblies must be changed if they are accidentally damaged or attacked chemically figures 23 and 24 You should also remove them for cleaning or decontamination purposes and to lubricate the pistons 1 Examine the ejector cover if it is cracked or badly corroded it should be replaced with a new part Otherwise it should be cleaned and if necessary autoclaved 2 Check the piston for damage or corrosion Lubricate and replace as necessary 3 After removing the locking clip gently pry out each tip holder s internal components lock Seal halides ing clip spring O ring and seal holder figure Y Locking clip 25 this can be done using the reassembly tool Replace or clean and decontaminate these components Replace or clean and decontaminate the tip holder Tip holder spring LS The O ring can get trapped in tip holder Use Figure 25 a fine plastic probe to extract the O ring Piston spring
35. ode is indicated To select another mode scroll the different modes by turning the thumbwheel then press the thumbwheel to confirm In each of the modes listed below the parameters last used are kept in memory until you change them IS After a few seconds the display returns to the last mode used if no new mode is selected The REVERSE mode can be replaced by another programmed mode that can be uploaded by the user with a PC Connection Kit optional The kit which includes a PC cable programming and configuration software can be obtained from your usual Gilson representative the reference number is F30755 Reminder Pressing both mode buttons at the same time switches the pipette to System menu see Chapter 10 Aborting the Active Mode If you press one of the mode buttons during the current cycle the following is displayed If you do not press the START button within a few seconds you are sent back to the previous screen AUTO AUTO MIX MANUAL REPETITIVE REVERSE PIPETMAN Concep WARNING Process aborted Press START to Blow Out PIPETMAN Concep MN PIPETMAN Conce RS Mode Description AUTO a Description This is the simplest mode Aspirate and dispense are initiated using the START button b Protocol i Click on the thumbwheel to unlock the volume setting the current volume will flash li Set the sample volume turn the thumbwheel until you see the selected volume displayed
36. older 23 25 27 Low quality tips 14 15 Damaged tip holder 23 25 27 Damaged tip ejector 24 26 Pipette is not switched on T Battery needs recharging 4 MN PIPETMAN Conce RS 1 5 ACCESSORIES 8 REPLACEMENT PARTS Accessories Item Rapid Charging Stand PC Connection Kit including PIPETMAN Concept Utility Software F30755 PIPETMAN Concept Utility Software Name Tag AC adapter Europe AC adapter USA Japan AC adapter UK AC adapter Australia Replacement Parts for PIPETMAN Concept Single channel Item Battery block Adjustable hook Personalization window Tip ejector assembly Piston assembly Return spring Spring guide Tip holder upper part O ring x 5 or seal for C10 Tip holder lower part Connecting nut Tip ejector extension for C10 Lubricant tube 3 5 g Bag of 10 filters Bag of 100 filters 0 KE KE 8 C10 F3071165 F3071110 F3071103 F2070159 F3071252 F2070113 F307115451 F307115451 F2070117 F161902 F2070218 F2072111 F2070903 C100 F3071165 F3071110 F3071103 F2070459 F3071352 F2070113 F2070417 F2070401 F2070418 F2072111 F3070902 ee C300 F3071165 F3071110 F3071103 F2070559 F3071452 F2070113 F307115451 F307145302 F307145351 F307145301 F2072111 F3070902 C1200 F3071165 F3071110 F3071103 F2070659 F3071552 F2070113 F307115451 F2070617 F2070601 F2070618 F2072111 F3070902 C5000 F3071165 F3071110 F3071103 F2070759 F3071652
37. on the screen then press the thumbwheel to lock the volume you cannot dispense until this is done Set the speed optional press the keys to increase or reduce the speed default is 5 The speed can be changed before or during a pipetting cycle Nr c Functionality i Immerse the tip in the liquid to be aspirated ii To aspirate click press and release the START button and wait until the motor stops check piston position indicator To dispense click press and release the START button Three things happen sample dispense automatic purge and piston reset to zero However if you keep the button pressed until the end of the dispense cycle when the motor stops the piston reset takes place after you release the START button again This feature enables you to remove the tip from the liquid otherwise liquid would be re aspirated when the piston resets Ner AUTO AUTO MIX MANUAL REPETITIVE REVERSE PIPETMAN Concept AUTO i 1200 Speed 3 amp Count 0 PIPETMAN Concep START button O Optional STOP LS In this mode the user has access to a cycle counter which is incremented after each pipetting cycle It counts up to 999 before starting again at zero The counter is reset to zero each time the volume is changed or by pressing twice on the thumbwheel AUTO MIX AUTO a Description ie ms This mode operates in the same way as AUTO but is followed REPETITIVE by a mixing pha
38. ons mentioned in Decontamination Procedures for Gilson Pipettes reference LT802288 External 1 Remove the tip ejector see Chapter 11 2 Wipe the tip ejector with a soft cloth or lint free tissue impregnated with soap solution 3 Wipe the entire pipette with a soft cloth or lint free tissue impregnated with soap solution to re move all dirty marks If the pipette is very dirty a brush with soft plastic bristles may be used 4 Wipe the entire pipette and the tip ejector with a soft cloth or lint free tissue impregnated with distilled water Internal The following components only can be immersed in a cleaning solution tip ejector tip holder both parts connecting nut piston including holder return spring and spring guide 1 Disassemble the pipette as described in Chapter 11 2 Set aside the upper part in a dry and secure location 3 Clean the individual components of the lower part of the pipette using an ultrasonic bath 20 minutes at 50 C or with a soft cloth and brushes Small round brushes with soft plastic bristles may be used to clean the interior of the tip holder 4 Rinse the individual components with distilled water 5 Leave the parts to dry by evaporation or wipe them with a clean soft cloth or lint free tissue 6 Lubricate the piston and reassemble the pipette according to the instructions given earlier in the previous chapter Decontamination Autoclaving The handle of the pipette is not
39. pette 5 Storage conditions Temperature 20 C to 50 C Humidity max 80 6 Temperature of use Between 4 C and 40 C specifications may vary 7 Use only original Gilson s DIAMOND tips accessories and spare parts If the equipment is used in a manner not specified by Gilson in the user s guide the protec tion provided in the equipment may be impaired 8 Equipment disposal This equipment must not be disposed of with unsorted municipal waste Instead it is your responsibility to correctly dispose of your waste equipment by handing it over to an authorised facility for separate collection and recycling It is also your responsibility to decontaminate the equipment in case of biological chemical and or radiological contamination so as to protect from health hazards the persons involved in the disposal and recycling of equipment es For more information about where you can drop off your waste equipment for recycling please contact your local dealer from whom you originally purchased the product or your local council By doing so you will help conserve natural resources and you will ensure that your waste equip ment is recycled in a manner that protects human health and the environment Thank you 35 MN PIPETMAN Conce RS 1 F SPECIFICATIONS PIPETMAN Concept is a high quality pipette that offers excellent accuracy and precision The figures given in the Gilson Maximum Permissible Errors table were
40. s for the months are as follows JAN FEB MAR APR MAY JUN JUL SEP OCT NOV DEC Maintenance Alarm Y Cycles 20000 gt Weeks 36 Date 18 JAN 07 Alarm ON Quit PIPETMAN Concep By default the maintenance alarm is set to OFF rotating the thumbwheel alternates between On and Off confirm by pressing the thumbwheel Warning messages will be displayed depend ing on the maintenance settings that you make see Chapter 11 Pipette info Disassembly This screen is read only you cannot modify any of the items which are ae Service settings The serial number of the pipette da The version of the firmware ae ds The number of cycles since the pipette was manufactured PIPETMAN Concept Personalization This menu allows you to personalize the standby screen Select New ld With the cursor aligned as shown gt A click and Configuration turn the thumbwheel to select a aonb aintenance character and click it to confirm gt Personalization You may enter a string of up to 15 jasi alphanumeric characters Use the asterisk to signal that you have en teredthelastcharacter use to cancel PIPETMAN Concep e S N Z10001A Firmware V2 10 Total cycles 41353 gt Quit PIPETMAN Concep Id PIPETMAN Concept New ld gt A confirm cancel Quit PIPETMAN Concep 1 1 USER MAINTENANCE It is best to inspect your pipette regularly and to routinely clean and change p
41. se which is achieved by repeatedly aspirating dla a de PIPETMAN Cp b Protocol i Set the sample volume with AUTO flashing on the screen click the thumbwheel then turn it until you see the selected volume displayed then press the thumbwheel to lock the volume li Set the mix volume with MIX flashing on the screen turn the thumbwheel until you see the selected volume displayed then press the thumbwheel to lock the volume Then AUTO is displayed again The default is the sample volume previously selected to accept this value for mixing simply press the thumbwheel for the second time iii Set the speed optional press the keys to increase or reduce the speed default is 5 The speed can be changed before or during a pipetting cycle c Functionality i Immerse the tip in the liquid to be aspirated li To aspirate click press and re lease the START button and wait until the motor stops check piston position indicator iii To dispense click press and re lease the START button Three things happen sample dispense automatic purge and piston reset to zero However if you keep the button pressed until the end of the START button c Optional STOP Start Purge dispense cycle when the motor stops the piston reset takes place after you release the START button again iv To mix press the START button again mixing continues for as long as you keep the START button pressed Release the STAR
42. sed until the end of the dispense cycle when the motor stops the reset takes place after you release the START button again gt Also you can abort the cycle and purge by pressing one of the mode buttons MN PIPETMAN Conce RS REVERSE a Description This mode is like reverse mode pipetting using a manual pipette and recommended for viscous liquid Please refer to the Gilson Guide to Pipetting for further information b Protocol i Set the sample volume turn the thumbwheel until you see the selected volume displayed on the screen then press the thumbwheel to lock the volume you cannot dispense until this is done li Set the speed optional press the keys to increase or reduce the speed default is 5 The speed can be changed before or during a pipetting cycle c Functionality i Immerse the tip in the liquid to V extra vol be aspirated li To aspirate press the START button wait until the motor resets and stops at the end of the aspiration phase To dispense press and release the START button iv BLOW OUT appears on the screen click on START to purge Nr AUTO AUTO MIX MANUAL REPETITIVE REVERSE PIPETMAN Concent REVERSE i 1200 Speed 3 amp Count 0 PIPETMAN Concept and reset the piston However START button O Optional STOP Start Purge if you keep the button pressed until the end of the dispense cycle when the motor stops the reset takes plac
43. select the requested mode 6 A button to inverse direction of piston motion Manual mode 7 Start button to aspirate and dispense A button for tip ejection Screen LCD Typically the LCD screen is used to display The available modes and the parameters required for a specific mode Information relating to the current manipulation in real time Battery state 4 levels Piston direction and motion in real time see Chapter 6 Configuration and maintenance information see Chapter 10 Cycle counter see Chapter 9 Warning messages which appear in the place of volume refer to Chapter 14 for the full list Figure 4 Piston Position KK Battery Volume State Mode and Units ATO i 1 200 Speed 3 Count 0 rola Gne Speed Piston Cycle Direction Counter Tip ejector Extension only with C10 model In order to eject D10 tips tip ejector extensions are supplied with C10 pipettes figure 5 The tip ejector extension which is made of PVDF polyvinylidene difluoride is autoclavable Do the following to fit a tip extension 1 Hold the pipette with the LCD uppermost and hold the extension with the slot uppermost 2 Slide the extension over the tip holder 3 Push the extension firmly onto the end of the tip ejector until it clicks into place see opposite 4 To remove a tip ejector extension see opposite a Hold the pipette in one hand and grip the extens
44. tenance 2 Immerse the components in the decontaminant solution or wipe them according the instructions given by the manufacturer or supplier of the decontaminant 3 Rinse the individual components with distilled or sterile water 4 Leave the parts to dry by evaporation or wipe them with a clean lint free tissue or soft cloth 5 Lubricate the piston and reassemble the pipette according to the instructions given in this chapter 1 3 LEAK TEST This test may be performed at any time to check that the pipette does not leak especially after performing a maintenance or decontamination procedure lf a pipette fails this test you should replace the O ring and seal and repeat this test after making sure that the pipette is correctly reassembled C10 to C300 Single and Multichannel fita Gilson DIAMOND Tip set the pipette to the maximum volume given in the specifications and pre rinse aspirate the set volume from a beaker of distilled water maintain the pipette in the vertical position and wait for 20 seconds if a water droplet appears at the end of the tip there is a leak e if you see no droplet re immerse the tip below the surface of water the water level inside the tip should remain constant if the level goes down there is a leak C1200 to C10ml fit a Gilson DIAMOND tip set the pipette to the maximum volume given in the specifications aspirate the set volume from a beaker of distilled wa
45. ter maintain the pipette in the vertical position and wait for 20 seconds if a water droplet appears at the end of the tip there is a leak 1 4 TROUBLESHOOTING In case of malfunction first press the reset button located in the back of the pipette between the power supply and the PC connec tor Insert a small plastic probe like a tip in the hole and press If the problem persists you may consult the following table wich identifies potential problems and their solutions Before returning any pi A pette to your local Gilson Service Center ensure that it is completely free of chemical biological or radioactive contamination Please use the included safety bag Symptom Pipette is leaking sample Pipette won t aspirate Noisy operation Pipette is inaccurate Pipette is not precise Tips fall off or don t fit No LCD display cds Possible Cause Refer to page 23 24 26 Worn O ring 23 24 26 Unscrewed lower part of tip holder 23 Worn O ring Damaged or corroded piston 24 26 Damaged tip holder 23 20 21 Improper repair or assembly 22 27 Connecting nut is loose 29 Piston needs lubricating 21 Improper repair or assembly 22 27 Unscrewed lower part of tip holder 23 Pipette is out of adjustment 18 20 Connecting nut is loose 25 Unscrewed lower part of tip holder 23 Incorrect operator technique 15 16 Worn O ring 23 24 26 Connecting nut is loose 25 Damaged or corroded piston 24 26 Damaged tip h
46. ter 3 minutes of inactivity Press any button to reactivate the pipette If you do not press one of the buttons within 3 minutes of the end of the current cycle you will see the standby screen When you next press a button the software reinitializes the piston 4 DESCRIPTION Pipette figure 3 1 Adjustable tip ejector button can be positioned for left or right handed operation Ergonomic adjustable hook to make the pipette more comfortable to use and to reduce fatigue 3 Identity tag window see GLP features 4 Connecting nut links handle to the lower part 5 Tip ejector stroke adjustment wheel Force Regulator 6 Tip ejector removable to access piston assembly and tip holder Optimized tip holders to reduce tip fitting and ejection forces removable for cleaning and servicing 20 300 uL Pipetman Concept Initializing V2 10 PIPETMAN Concept 20 300 uL Pipetman Concept On stand by V2 10 PIPETMAN Concept Figure 3 MN PIPETMAN Conce RS Keypad figure 4 The following buttons are used to control the pipette Thumbwheel to set the volume and other system parameters setting by rolling and validating by clicking Q Reset button to initialize the pipette see chapter 5 3 Contacts for charging the battery from the stand see chapter 15 4 Two buttons and to adjust the speed 5 Two mode buttons one for right handed and one for left handed to
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