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Instruction manual Isothermal Carousel freezer

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3. 5191043402 M q uone iunululo JONUOWOAID 685 64 101 5 LNO S8r SY S19 O1JUOD 7 UdIMJ9q JOUUOWOAID 685 63 JO P SN NI 68 58 woisuedxe 40J 19449433 94 340d 3 3 3NVd ACIS 43 TIOULNOD LOEC 531435 tN 4 C_ EBS www custombiogenics com V SERIES CAROUSEL MANUAL FILL INSTRUCTIONS The unique design of the new Isothermal Storage Unit is such that it is no longer necessary to fill the storage chamber with liquid nitrogen Instead the inner walls of the storage vessel are filled with liquid nitrogen and the sample storage chamber holds no liquid at all Because of this unique design the manual filling of this equipment is very different from storage units of the past Use the following instructions to manually fill the Isothermal Carousel Storage unit in the event of a power outage or if the auto fill is not working properly For the correct manual filling of the Isothermal unit perform the following steps 1 Turn off the liquid nitrogen supply valve at the supply tank 2 Disconnect the supply line from the Auto fill port on the back of the unit AUTO FILL CONNECTION MANUAL FILL CONNECTION 4 Place the cap on the auto fill port where the supply line was connected and tighten down 5 Connect the liquid nitrogen supply line to the manual fill port and tighten down www c
4. Clockwise adjusts temp display down Counter clockwise adjusts temp display up Continue adjusting back and forth between steps 2 and 3 until temperature is correct on both ends Zero and Span without further adjustment Calibrate to 1 degree BYPASS TEMP NO UJ JI Satisfy alarm conditions so that the controller is in its normal operating state Examples Connect valves and thermocouples set up low high set points and temperature alarm set point Place BYPASS thermocouple probe in an Ice Bath and adjust trimmer P6 until display reads 0 deg C Clock wise adjusts temp display down Counter clockwise adjusts temp display up Place BYPASS thermocouple probe in Liquid Nitrogen and adjust trimmer P7 until display reads 196 deg C Clockwise adjusts temp display down Counter clockwise adjusts temp display up Continue adjusting back and forth between steps 2 and 3 until temperature is correct on both ends Zero and Span without further adjustment Calibrate to 1 degree TEMP B TEMP A VENT TRIMMERS iu j PRA UL LL z AE 2 m Eur era gt 4 i i i T IN HIT it ine L Go to CustomBioGenics com technicalguides html for more information www custombiogenics com 97 eS Ss V SERIES CAROUSEL LIQUID LEVEL DISPLAY VERIFICATION amp CALIBRATION Since the V Series storage un
5. Back Panel Connections 8 Cleaning amp Maintenance 39 Intended Use 40 www custombiogenics com CES pe RECOMMENDED BEST PRACTICES Custom BioGenic Systems Recommended Best Practices for safe product storage Secondary or backup alarm It is strongly recommended to have at a minimum an Independent Temperature Alarm for each LN2 freezer Remote alarm connection It is strongly recommended to have at a minimum a Remote Auto Dialer connected to your Delta Room Facility Monitoring Station or Remote Auto Dialer for each LN2 freezer Split Samples Separate samples in to two different freezers Keep a daily log Track temperature levels daily Record fill intervals and amount of LN2 filled into vessel manual or pour fill freezer or dewar This information should be documented daily and reviewed monthly to foresee and prevent future problems such as temperature fluctuations and varying liquid levels Prepare a contact list Have at least 3 people on your contact list with Home Cell and Pager Number Review the list regularly for accuracy and changes Contingency plan What to do if no one returns a call from your call list What to do if we run out of LN2 and it is a weekend or holiday What to do if you have a High Temp Alarm What to do if you have a Low Level Alarm What to do if you have a High Level Alarm What to do if you lose power What to do if you get a Source Alarm www custombiogenics com C
6. E L5 QUESTIONS and ANSWERS Keep your most important irreplaceable product safe You can plan for the future you can t change the past Back up or secondary Temp Alarm Why do need a Secondary or Back Up Alarm A Your freezer is a mechanical device and could malfunction By having a secondary or back up alarm you can minimize the possibility of a loss due to a malfunction Q Why a Temperature Alarm and not a Liquid Level Alarm A Your LN2 freezer and specimens depend on the temperature of the freezer Even though you are using the liquid level to control the temperature of your freezer and keep your specimens safe it is the more efficient to detect changes the temperature In order to prevent cross contamination vapor or dry storage is becoming the preferred method of storing samples Therefore the samples are not immersed in to liquid nitrogen and monitoring the temperature is the most important variable An independent temperature verification may be required Remote Alarm Connection to your Delta Room Facility Monitoring Station or Remote Auto Dialer Why should use my remote alarm feature or connection A If an alarm occurs after hours on a weekend or holiday the remote alarm connection will alert you to a problem and let you address it quickly Q lam not sure how to connect the alarm or what to connect the alarm to A First contact your lab manager supervisor or in house biotech representative If
7. check to see that the valve is plugged in and or that there is power to the outlet which the valve is plugged into If power is present at the valve but the vessel is still not filling with liquid nitrogen the valve may be defective An automatic fill will begin and when the liquid level rises above the LOW level setpoint and the pressure port the alarm will subside Check pressure hose at all connection points a white clamp should be in place at each end of the hose inside the controller and at the hose connection on the back of the controller If hose appears loose disconnect it remove an inch off the end by cutting it with scissors and reconnect it If the vessel is holding liquid it may take some time to read the corrected level gt E lt gt TROUBLESHOOTING GUIDE ALARMS CONDITIONS SOURCE text is flashing and audible alarm is present ALARM SOURCE ALARM This indicates a weak or empty liquid supply Liquid level is above the LOW level set point but isn t rising fast enough to satisfy the system TEMP A text is flashing and audible alarm is present ALARM TEMP A NOTE Text may display Temp B OPEN FILL text is flashing and audible alarm is present ALARM OPEN FILL www custombiogenics com CAUSES Supply tank is empty Supply tank is shut off Supply tank pressure is too low or to high 3 or more storage units are filling at one t
8. or 15 selected on display 19 Use this menu to select a temperature for the Temp A Alarm to be activated This display will appear when F 15 selected on display 19 Use this menu to select a temperature for the Temp B Alarm to be activated This ED This display will appear will appear when REPORT is selected on display 5 Use this menu to select the time interval between temperature reports NOTE A value of means temp report printing is disabled For use with thermal printer only This LL ese This display will appear when will appear when ENTER is selected on display 22 Use this menu to select number of days between temperature reports NOTE A value of means temp report printing is disabled For use with thermal printer only gt E lt gt LCD DISPLAY FUNCTIONS Program Mode Display Windows This display will appear when BYPASS is selected on display 5 This menu is a prompt to enable or disable the Bypass Option Press this button to Press this button to change current setting maintain current setting and return to display and return to display 4 MAIN MENU 4 MAIN MENU see page 16 see page 16 This display will appear when 40 VALIDATION is selected on display 6 This menu begins the testing of the overlay buttons Select BEGIN to proceed or SKIP to advance to the next
9. window of the validation process Press this button to Press this button to begin testing the skip test and overlay buttons and advance to display advance to display 41 see below 41 and begin the final test see below This This display will appear when SKIP is will appear when SKIP is selected on display 40 or upon completion of testing the overlay buttons This menu tests the audible alarm This is the first in a series of test menus which will appear sequence when PASS or SKIP is selected Press this button to Press this button if Press this button if Press this button to test audible alarm test passes to test fails to advance skip test advance for each function being tested Buzzer should sound advance to display to display to display 441A 141A and next test and next test and next test below below below This display will appear when PASS FAIL or SKIP is selected on display 40 This menu tests remote alarm contacts Controller advances to next test when PASS FAIL or SKIP is selected Press this button to Press this button if Press this button if Press this button to toggle remote alarm test passes to test passes to skip test advance contacts advance to display advance to display to display 41 41B and next test 41B and next test and next test see page 21 see page 2
10. SHOULD ALWAYS WEAR A FACE SHIELD AND PROTECTIVE CLOTHING LIQUID NITROGEN SHOULD NEVER BEUSED IN COMBINATIONWITH OTHERSUBSTANCES WITHOUT KNOWING WHAT THE RESULT WILL BE WHEN IN DOUBT CONTACT A COMPETENT AUTHORITY www custombiogenics com 1 C E L5 LIQUID NITROGEN SAFETY HANDLING LIQUID NITROGEN Personnel handling liquid nitrogen should be thoroughly instructed as to the nature of these materials Proper training is essential to safety and will ensure the accident free use of this equipment Because of its low temperature liquid nitrogen will cause frostbite to the skin much in the same way as hot liquids can burn For this reason always wear the proper protective clothing when handling these materials It is advised that during use handlers of liquid nitrogen should protect themselves by wearing goggles or face shields cryogenic gloves large enough to allow quick removal and a cryogenic apron It is preferable that shoes worn at these times have high tops as to not permit accidently spilled liquid from entering as well as pant legs which come down over the tops of shoes for further protection Also because of the extremely low temperatures liquid nitrogen should only be handled and transported in approved containers Many materials become brittle and may shatter when put into contact with liquid nitrogen FIRST AID In the event a person has a frostbite from liquid nitrogen the following first aid treatment should
11. Timer setting Lid Temperature Display Lid Temperature Thermocouple is located approximately 11 from the top of the tank Lid Temperature Thermocouple is located approximately 20 from the top of the tank 0 5Vdc amp 4 20mA Outputs Easy screw terminal output connections for existing alarm and monitoring systems This feature is available through the Tank ID menu See page 16 screen 4 Select either 0 5 with connections to Temp A Temp B and Level 4 20mA with connections to Temp A Temp B and Level Fill Vent In Progress Signal to activate the Custom Biogenic Systems TS 1B LN2 supply tank switcher or an additional 24Vdc valve to control the LN2 supply Report Log Feature See page 15 screen 99 Alarms Records Sequence of events such as POWER ON etc Data The LOG function is enabled for use with either Cryomonitor or Reporting This will Date Time stamp Temp A Temp B and Level at the programmable rate of 1 90 hours on the hour This information is selected by date range to either be displayed or printed for easy review Optional Features Overflow Sensor Not included as standard equipment can be purchased separately This feature uses a thermister set at the bottom of the storage space along the probe holder tube If an overflow occurs it will cause the LN2 to make contact with the thermister An audible and visual alarm will occur and SYSTEM STATUS ALARM LN2 OVERFLOW will a
12. no one is sure what to do contact CBS customer service for help and direction What is an Auto Dialer and why would 1 use one A An Auto Dialer is used when a central connection is not available for the remote alarm When connected to a telephone land line the Auto Dialer will dial up to 8 different contacts If you have any question comments or concerns please contact Custom BioGenic Systems Customer Service Phone 586 331 2600 Toll free US only 800 523 0072 Email sales custombiogenics com www custombiogenics com C E L5 LIQUID NITROGEN SAFETY WARNING LIQUID NITROGEN IS AN EXTREMELY COLD LIQUID LIQUID NITROGEN EXISTS AT 196 C BECAUSE OF THESE COLD TEMPERATURES LIQUID NITROGEN WILL CREATE A FROSTBITE IF IT COMES INTO CONTACT WITH SKIN NEVER ALLOW DIRECT SKIN CONTACT WITH LIQUID NITROGEN OR SERIOUS FROSTBITE WILL RESULT ALTHOUGH LIQUID NITROGEN ITSELF IS NON TOXIC WHEN RELEASED INTO A CONFINED SPACE IT CAN DISPLACE OXYGEN CAUSING ASPHYXIATION ENTERING AN OXYGEN DEFICIENT ROOM CAN CAUSE UNCONSCIOUSNESS WITHOUT WARNING ALWAYS CHECK AIR QUALITY UPON ENTERING A ROOM WHERE CRYOGENIC LIQUIDS ARE BEING USED AND IF POSSIBLE HAVE AIR RESPIRATORS AVAILABLE INTRODUCING EQUIPMENT WHICH IS AT ROOM TEMPERATURE INTO LIQUID NITROGEN IS ALWAYS SOMEWHAT HAZARDOUS BEWARE OF SPLASHING AND BOILING WHICH MAY OCCUR ALL PERSONNEL PERFORMING THESE OPERATIONS SHOULD BE FULLY INFORMED OF PROPER HANDLING PROCEDURES AND
13. of some kind and the alarm MUTE is activated Thermistor plug is disconnected Thermistor is damaged 32 SOLUTIONS Connect bypass valve power cord correctly using diagram on page 7 Replace bypass valve Connect probe plug properly using diagram on page 7 Replace damaged probe Connect probe plug properly using diagram on page 7 Replace damaged probe Correct the alarm condition and then push the SOUND switch so the audible alarm will be activated at the next alarm occurrence Connect Thermistor properly using diagram on page 7 Replace damaged Thermistor gt E lt gt TROUBLESHOOTING GUIDE ALARMS CONDITIONS LN2 OVERFLOW test is flashing and audible alarm is present NOTE This is an optional function additional equipment is required If overflow alarm is activated power to the solenoid valve is disabled ALARM LN2 OVERFLOW Liquid Nitrogen has flooded vessel through internal vents V Series units only No lights Liquid level display showing incorrect reading Remote alarm working improperly or not at all www custombiogenics com CAUSES Liquid level has become too high inside the vessel walls because of stuck solenoid user error etc Disconnected sensor tube see 7 on page 7 EH settings are enable on a non EH unit High pressure supply Liquid level has become too high inside the vessel walls because of stuck solenoid user error
14. on display 5 Use this menu to select temperature preferences i e F or C Press this button to Press this button to Press this button to select degrees select degrees discard changes and Fahrenheit and Celsius and advance return to display 4 advance to display 20 to display 20 MAIN MENU see page 19 see page 19 see page 16 www custombiogenics com 18 CBS LCD DISPLAY FUNCTIONS Program Mode Display Windows These arrows cs value Press button to Press button to save changes discard changes and advance to display return to display 4 21 see below MAIN MENU see page 16 These arrows adjust settings 7 _ Press this button to Press this button to save the setting and discard changes and Return to display return to display 4 4 Main Menu MAIN MENU see page 16 see page 16 These arrows adj zS value Press this button Press this button to save changes discard changes advance to display return to display 5 23 below MAIN MENU 2 see page 16 These arrows adj v3 value ee Press this button to Press this button to Press this button to print report instantly save changesand discard changes and return to display 5 return to display 5 MAIN MENU 2 MAIN MENU 2 see page 16 see page 16 www custombiogenics com 19 This display will appear when F
15. setting and return to display 4 MAIN MENU see page 16 These arrows 7 jJ Press this button to Press this button to savethesetting and discard changes and go to display 13 return to display OUTPUT SIGNALTYPE 13B Output Signal see below Type see below These arrows adjust settings 7 Press button to save changes and return to display 4 MAIN MENU see page 16 Press this CJ to discard changes and return to display 4 MAIN MENU see page 16 Press this button to Press this button to save the setting and discard changes and Return to display 5 return to display 5 Main Menu 2 MAIN MENU 2 see page 16 see page 16 These arrows www custombiogenics com 23 adjust settings adjust settings This display will appear when you hit the REV button on display 6 on PAGE 16 Use this menu to view your revision number and date This display will appear after a selection is made on screen 13 see page 18 NOTE If this controller is connected to the PC it is to be set to master if it is in the chain leading to the master it is to be set to slave This display will appear after a selection is made on screen 13A see above Use this menu to activate either the 4 20mA outputs or the 0 5V outputs NOTE Outputs are for use with an external monitoring system through the 16 port connector This display wil
16. when PASS FAIL or SKIP is selected This display will appear when PASS FAIL or SKIP is selected on display 41B This menu is for testing the lid temperature Controller advances to next test when PASS FAIL or SKIP is selected This display will appear when PASS FAIL or SKIP is selected on display 41B 2 This menu displays current vent temperature Controller advances to next test when PASS FAIL or SKIP is selected This display will appear when PASS FAIL or SKIP is selected on display 41C This menu tests printer Controller advances to next test when PASS FAIL or SKIP is selected gt E lt gt LCD DISPLAY FUNCTIONS Program Mode Display Windows Press this button to Press this button if Press this button if Press this button to activate the Bypass test passes to test fails to advance skip test advance Valve for as long as advance to display to display 41F and to display 41F the button is held 41 and next test next test and next test below below below 41F Press this button to Press this button if Press this button if Press this button to activate Fill Valve test passes to test fails to advance skip test advance for as long as the advance to display to display 416 and to display 41G button is held 41G and next test next test and next test below below below 41G Press this button if Press this button if Pr
17. 1 see page 21 www custombiogenics com 20 CBS LCD DISPLAY FUNCTIONS Program Mode Display Windows u ur Press this button if Press this button if Press this button to test passes test fails to advance skip test advance advance to display to display 41B 2 to display 41B 2 41B 2 and next test next test and next test below below below Press this button if Press this button if Press this button to test passes to test fails to advance test advance advance to display to display 41C and to display 41C 41C and next test next test and next test below below below hm Press this button if Press this button if Press this button to test passes to test fails to advance skip test advance advance to display to display 410 and to display 410 410 and next test next test and next test below below below BEEN Press button to send TESTING PRINTER to the Press button if Press amu buttonif Press button to test passes to test fails to advance skip test advance advance to display to display 41 to display 41E printer 41E and next test next test and next test see page 22 see page 22 see page 22 www custombiogenics com 21 This display will appear when PASS FAIL or SKIP is selected on display 41A This menu is for testing the lid temperature Controller advances to next test
18. 8 This display will appear when NEXT is 5 selected display 4 Press this button to Press this button to Press this button to Press this button to advance to display advance to display advance to display advance to display 19 LID TEMP 22 REPORT 24 BYPASS 6 MAIN MENU 3 settings settings settings see below see page 18 see page 19 see page 19 This display will appear when NEXT is 6 selected on display 5 Press this button to Press this button to Press this button to Press this button to advance to display advance to display advance to display return to display 40 VALIDATION 74 FILL TIMER 42 REVISION 4 MAIN MENU settings settings parameters see above see page 19 see below see page 22 This display will appear when NEXT is selected on display 6 7 ss this button to Press this button to Press this button to advance to advance to screen operation and Display 44 45 see page 25 return to display 4 see page 24 MAIN MENU see above www custombiogenics com 16 CB Ss LCD DISPLAY FUNCTIONS Program Mode Display Windows Press this button to Press this button to ENABLE Fill Timer DISABLE Fill Timer this willadvance this will return you you to display to display 6 7 below Main Menu 3 see page 16 7 Press this button to Press this button to Press this button to Press this button to select a 24 hour time selecta 48 hour time s
19. CUSTOM BIOGENIC SYSTEMS ISOTHERMAL CAROUSEL FREEZER 2301 OPERATORS MANUAL CBS 3000 48 Custom www custombiogenics com BioGenic 150 Shafer Drive Romeo 48065 USA GB Systems 500 523 0072 tel 1 586 331 2600 Fax 1 586 331 2588 Leading the World with Innovative Cryopreservation Technology Solutions European Authorized Representative EMERGO GROUP Molenstraat 15 2513 BH The Hague The Netherlands IMPORTANT INFORMATION We at Custom Biogenic Systems are proud of our work and appreciate your purchase of this product With proper care this equipment will be trouble free for many years to come Before setting up and using your new cryogenic storage unit first check to see that all parts are accounted for and that no damage has occurred during shipping Also read this manual completely before proceeding to set up If at any time you are unsure of the procedures for set up and use of this product please contact CBS or your CBS sales representative NOTE If equipment is used in a manner not specified by Custom Biogenic Systems the protection provided by the equipment will be impaired PRODUCT WARRANTY Custom Biogenic Systems warrants all manufactured cryogenic equipment to be free from defects in workmanship or materials for a period of three years Custom Biogenic Systems liabilities under the warranty shall be limited to correcting or replacing defective workmanship or materials A claimant under the warr
20. ENTER button was pressed Note Factory settings should be kept any questions please contact Custom BioGenic Systems This display will appear after screen 46 see above Note Factory settings should be kept any questions please contact Custom BioGenic Systems COC BS HIGH amp LOW LEVEL ALARM AND TEMPERATURE DISPLAY VERIFICATION The following section includes verification procedures for the High and Low Level Alarms and the Temperature Display on the Isothermal Carousel Liquid Nitrogen Storage Units Low Level Alarm Verification 1 Using the manual valve on the liquid nitrogen supply tank turn off the liquid supply 2 Create low level alarm condition This is done by adjusting the level set points above the actual liquid level If necessary refer to page 18 display 15 and 16 Example The liquid level is 6 inches Adjust the high level set point to 10 and the low level set point to 8 This will cause the controller to go into an auto fill In 7 to 10 minutes low alarm should occur This will be indicated by the LOW ALARM text flashing and the audible alarm sounding The remote alarm output will activate also 3 Return set points to the desired settings 4 Open manual valve on supply tank to resume normal operation High Level Alarm Verification 1 Create a high level alarm condition This is done by adjusting the level set points at lease one inch below the actual liquid level If necessary r
21. V3000EH AB C LN2 Capacity Lites Static Holding Time Days _ 8 _ __ 2 _ External Dimensions Width ifm A PIT tnn 48 1 _ 48 1 219 Height 44 5 1 130 54 5 1 384 Usable Interior Height CY 27 686 35 889 Usable Interior Diameter Dub 29 737 29 737 Weight Empty UZLE 600 272 636 288 Weight Full 5 720 327 795 361 Maximum Vial Capacity 2m 5 18000 22500 Maximum Blood Capacity 852 1136 Operating levels for LN Extra High Series 0 to 28 inches higher fill heights may be used call CBS for more info V Series Models 23 inches www custombiogenics com 12 gt gt SS LCD DISPLAY FUNCTIONS This section shows each display window and gives important information necessary for understanding and operating the LCD text functions of the 2301 Controller To start the index below shows where in this section to find each and every function of the controller This will enable the user to correctly program all functions of the storage unit to meet their individual needs The overall operation consists of two basic modes the Standard Operating Mode and the Program Mode The unit is in the Standard Operating Mode when the Program Lock key switch on the front panel Page 6 number 9 is turned to Lock To enter the Program Mode the key must be inserted and the switch turned to Program In the Standard Operating Mode there are 6 different disp
22. W IS A GENERAL 1 IDENTIFICATION NUMBER USED TO ORGANIZE THE DISPLAYS FOR THIS MANUAL THIS NUMBER WILL NOT Press this button to Press this button to begi fill advance to display 99 CONTROLERORTHEACTUAL WHYITIS OCCURRING DISPLAY WINDOWS page 13 THE TEXT BELOW THE BUTTONS EXPLAIN THE FUNCTION OF EACH OF THE BUTTONS ALSO TELLS WHICH DISPLAY THE BUTTON WILL ADVANCE TO AND THE PAGE NUMBER WHERE IT CAN BE FOUND IN THE INSTRUCTION MANUAL Standard Operating Mode Display Windows This is the standard display 1 which will appear during normal operation under most circumstances NOTE Use Up Down arrows to toggle display between Temp A and Temp B This display will appear during normal operation when either a manual or automatic fill is occurring www custombiogenics com 14 lt gt es LCD DISPLAY FUNCTIONS Standard Operating Mode Display Windows This display will appear during normal operation when the lid is opened Cf JC JC Press this button to Press this button begin a manual fill to stop filling This display will appear when an alarm condition is occurring The specific nature of the alarm will be shown in line 90 of the display text The ALARM will flash and off second intervals LOW ALARM is shown only
23. anty must notify Custom Biogenic Systems within 10 days after the discovery of the defect Custom Biogenic Systems reserves the right at their discretion to correct the defect s in the field without return shipment to the factory This warranty does not cover defects on cryogenic equipment resulting from abusive handling and subsequent structural failure Serial Number Model number For Technical Assistance Call 1 800 523 0072 U S Only Phone 586 331 2600 Fax 586 331 2588 www custombiogenics com amp t www custombiogenics com gt RB Ss lt TABLE OF CONTENTS Safety Pagel V Series Carousel Vessel Descriptions 3 Initial Set Up 4 Operation Cryomonitor Software 5 Front Panel Controls ALL 6 2301 Carousel Side Panel 7 V Series Manual Fill Instructions 9 Feature Details 1 Remote Alarm Wiring 12 Storage Unit 12 LCD Display Functions 13 Standard Operating 14 Programming Mode 6 Verification amp Calibration High amp Low Alarm Set Point Verification 26 Temperature Display Verification 26 2301 Temperature 27 V Series Level Display Verification amp Calibration 28 Trouble Shooting 1500 Manifold amp Back Panel Connections 3 3000 Manifold amp Back Panel Connections 38 5000 Manifold amp
24. as an example of reset alarm If it re mute audible alarm the alarm display window See page 13 fora occurs repeat until all and advance to complete list of alarms alarms are corrected display 91 see below This display will appear when an 91 alarm condition is occurring AND the audible alarm is muted LOW ALARM is shown only as an example of the alarm display window See 13 for a complete list of alarms Press this button to Press this button to reset alarm If it re enable audible alarm occurs repeat until all and return to display alarms are corrected 90 see above This display will appear during normal 99 operation when REPORT is selected from displays 1 2 or 3 Press this C Press C button Press button and to view alarms toviewdataand the display will go back advance to display advance to display to whatever screen was 99 page 24 499A page 24 showing when the report was requested www custombiogenics com 15 CB Ss LCD DISPLAY FUNCTIONS Program Mode Display Windows This display will appear when the 4 PROGRAM LOCK switch is turned to PROGRAM Press this button to Press this button to Press this button to Press this button to advance to display advance to display advance to display advance to display 8 DATE TIME 13 TANK ID 15 LIQUID LEVEL 5 MAIN MENU 2 settings settings settings see below see page 17 see page 17 see page 1
25. be given while awaiting the arrival of medics or a doctor 1 If the material has contacted skin or eyes flood those areas with large quantities of unheated water and protect frozen areas with loose bulky dry and sterile dressings 2 If the skin is blistered or there is a chance that the eyes have been affected seek medical help immediately www custombiogenics com 9 L5 V SERIES CAROUSEL DESCRIPTION V SERIES ISOTHERMAL VESSEL Throughout this manual references are made to the V LIQUID NITROGEN IS CONTAINED INSIDE Series Carousel storage unit vessel In order to give a complete THE VESSEL WALLS AND DOES NOT understanding of the carousel model the diagram at the right is CONTACT THE INVENTORY SYSTEM provided With the V Series Carousel model the liquid nitrogen is contained inside the vessel walls and the stored samples inside the carousel never come into contact with the liquid nitrogen except in the case of an accidental overfill To understand the way the system operates see the OPERATION section on the next page for a basic explanation of the mechanics of the system Also be sure to read this manual completely before attempting to use this equipment If you are still not sure how to use this equipment properly contact a competent authority rather than risking your safety or the safety of the stored samples When the unit is placed into service check daily to ensure proper operation and safety o
26. eezing Syste tro cks Lines Transfer solenoid Valy perat vm port Tempe Contro tors rder Moni pee accessories Y 2301ISOLN2 TM Rev Org O 2001 2006 Custom BioGenic Systems All designs and materials contained herein are protected under Federal copyright law Unauthorized distribution or use will be subject to prosecution to the fullest extent of the law
27. efer to page 18 display 15 and 16 Example The liquid level is 6 inches Adjust the low set point to 2 and the high set point to 4 In 3 to 5 minutes a high alarm should occur This will be indicated by the HIGH ALARM text flashing and the audible alarm sounding The remote alarm output will activate also 2 Return set points to the desired settings Unit is now ready for normal operation If the alarm functions aren t occurring as described above repeat the verification procedure If one or both of the alarms are still not functioning properly refer to the troubleshooting guide on page 36 for further instruction Temperature Display Verification 1 Open Carousel storage unit Access Panel and locate temperature sensing probes A and B Access Panel c 2 Remove probe from protective sleeve LL 5 3 Submerge the sensor probe into liquid nitrogen The digital temperature display should read 195 to 196 degrees celsius 4 Remove probe from liquid nitrogen and place tip of probe in an ice bath mixture of ice and water and wait 30 seconds While the probe is submerged the digital display should read 1 to 1 degrees celsius 5 Return probe into its protective sleeve at the desired depth 6 Close the Carousel storage unit Access Panel and resume normal operation If the temperature display doesn t operate as described there may be a problem with the probe or the probe connections See the Troubles
28. electa 72 discard changes and interval and advance interval advance time interval and return to display 6 to display 7 below to display 7 below advance to display MAIN MENU 3 7 below see page 16 Press this buttonto Press this button to Press this button to advance to display move the curser advance to display 16 Main Menu 3 between 6 MAIN MENU 3 see page 16 Hours and Minutes see page 16 8 RN Press this button to Press this button to Press this button to move cursor left move cursor right return to display 4 MAIN MENU see page 16 www custombiogenics com 17 These arrows adj value This display will appear when FILL is selected on display 6 This is a prompt to ENABLE or DISABLE the Automatic Fill Timer The Fill Timer gives the option of an automatic fill every 24 48 or 72 hours at a specified time of day These settings are programmed in the 2 menus that follow This display will appear when ENABLE is selected on display Use this menu to set the time interval that the automatic fill will occur This 25 This display will appear will appear when any time interval is selected on display 78 Use this menu to set the time of day that the automatic fill will occur These arrows adj cs value This display will appear when DATE TIME is selected on display 4 Use this menu to change the da
29. ess this button to test passes to test fails to advance skip test advance advance to display to display 41H and to display 41H 41H and next test next test and next test below below below 41H Press this button to Press this button to print test results and return to display 6 MAIN MENU 3 see page 16 return to display 6 MAIN MENU 3 see page 16 www custombiogenics com 22 This display will appear when PASS FAIL or SKIP is selected on display 410 This menu tests the fill valve Controller advances to next test when PASS FAIL or SKIP is selected This display will appear when PASS FAIL or SKIP is selected on display 41E This menu tests the fill valve Controller advances to next test when PASS FAIL or SKIP is selected This display will appear when PASS FAIL or SKIP is selected on display 41F This menu tests the lid switch With the lid closed the display should read LID IS NOW CLOSED with the lid open it should read LID IS NOW OPEN Controller advances to next test when PASS FAIL or SKIP is selected This display will appear when the final test button is selected on display 41G and when all tests have been completed or skipped Use this menu to print test results if desired gt E lt gt LCD DISPLAY FUNCTIONS Program Mode Display Windows Press this button to maintain current
30. etc Disconnected sensor tube see 7 on page 7 EH settings are enable on a non EH unit High pressure supply Blown fuse No power at wall outlet AC power cord not plugged in ON OFF switch in OFF position Obstructed pressure port Loose sensor hose Unit out of calibration Remote alarm wires connected to the wrong terminals 33 SOLUTIONS Shut off manual valve on liquid supply wait for the liquid to settle and do one of the following Carefully bail liquid out of vessel using approved cryogenic container and proper personal safety equipment or Wait for liquid to evaporate from vessel on it s own Shut off manual valve on liquid supply wait for the liquid to settle and do one of the following Carefully bail liquid out of vessel using approved cryogenic container and proper personal safety equipment or Wait for liquid to evaporate from vessel on it s own Replace with two amp fuse Use live power source Plug the AC power cord into a wall socket Move power key switch into the ON position Clear blockage from pressure port in bottom of vessel Check sensor hose connection Calibrate unit using the calibration guide on page 29 30 Reconnect wiring using the guide on page 12 Remote Alarm Wiring gt E lt gt Fa P E piouajos gt 10549 MO JJ8AQ ZNI Jeuondo 4 III
31. f the stored samples For the V Series carousel units it is essential to lift the lid each day and check for vapor and signs of proper freezing www custombiogenics com 3 CBS INITIAL SET UP This equipment is designed to operate in the following environmental conditions Temperature 5 C 41 F to 40 C 104 F Humidity 80 at lt 31 C decreasing linearly to 50 at 40 C Altitude lt 2 000 meters 6 650 feet As a first step in the set up procedure be sure to read this manual completely to become more familiar with this equipment Next remove all packing materials and wipe unit with a clean cloth inside and out to remove any debris left from shipping Once the unit is wiped down complete the following Steps Note The Temp A Temp B alarm is set at 30 C from the factory Thus it will not alarm during the first fill and must be set by the user once the freezer cools 150 C 1 Connect the LN stainless steel flexible hose from the supply tank to the storage freezer but do not open the manual supply valve until instructed to do so Connect Remote Alarm or Auto Dialer if desired Plug unit into an appropriate power source Turn the power key switch to the ON position and the program lock to program Adjust the Liquid Level Set points See display 4 on page 16 for instructions press LIQ D level Adjust the High Temperature Alarm Set point after filling is complete See display 5 on page 16 for inst
32. from this sanitizing procedure cover all inner surfaces with the solution let stand for 30 minutes and remove Spraying the solution into the inner vessel is preferred although agitation of the solution inside the inner will sufice Rinse the decontaminated surfaces with clean water and make sure all cleaner residues have been removed Allow the tank to dry completely before putting back into service If you have any questions concerning decontaimination process please contact Custom BioGenic Systems Technical Support 1 800 523 0072 System Check 1 Testall alarm functions for proper operation 2 Checkany connected Remote Alarms or Automatic Dialing Systems to ensure proper operation See page 26 for detailed instructions on how to manually cause a HIGH ALARM or LOW ALARM to test the remote alarm contacts 3 Checkfor leaks at all connection points of the liquid nitrogen lines 4 Besurethat all electrical wires are free of damage and plugs are firmly in place SOLENOID VALVE MAINTENANCE The solenoid valves may be opened and cleaned if they are not working smoothly If this is done use the diagram below to reassembly the valves properly If the valve is found to be defective it must be replaced as replacement parts are not available individually 1 Valve Body 2 Plunger 3 Flange 4 Valve Coil Locknut 5 Coil 6 Spec Plate 7 Valve Cap www custombiogenics com 40 including Liquid Nitrogen Equ ms un Fr
33. hooting Guide on page 36 for further information if a problem is detected www custombiogenics com ea SS 2301 TEMPERTATURE CALIBRATION TEMP PROBE NO UJ JI Satisfy alarm conditions so that the controller is in its normal operating state Examples Connect valves and thermocouples set up low high set points and temperature alarm set point Place Temp A thermocouple probe in an Ice Bath and adjust trimmer P8 until display reads 0 deg C Clock wise adjusts temp display down Counter clockwise adjusts temp display up Place Temp A thermocouple probe in Liquid Nitrogen and adjust trimmer P9 until display reads 196 deg C Clockwise adjusts temp display down Counter clockwise adjusts temp display up Continue adjusting back and forth between steps 2 and 3 until temperature is correct on both ends Zero and Span without further adjustment Calibrate to 1 degree TEMP PROBE B NO UJ 4 Satisfy alarm conditions so that the controller is its normal operating state Examples Connect valves and thermocouples set up low high set points and temperature alarm set point Place Temp B thermocouple probe in an Ice Bath and adjust trimmer P10 until display reads 0 deg C Clock wise adjusts temp display down Counter clockwise adjusts temp display up Place Temp thermocouple probe in Liquid Nitrogen and adjust trimmer P11 until display reads 196 deg
34. ime from a single supply line Temperature has risen above set point Probe has been inadvertently moved Fill valve disconnected from power source Fill valve defective 31 SOLUTIONS Replace supply tank Open manual valve on supply tank if closed Check pressure in supply tank 25 p s i maximum 20 p s i minimum 22 p s i recommended Source alarms are not self correcting Lid has been open too long Low liquid nitrogen level Move probe to original location Connect fill valve power cord correctly using diagram on page 7 Replace fill valve gt E lt gt TROUBLESHOOTING GUIDE ALARMS CONDITIONS BYPASS text is flashing and audible alarm is present ALARM OPEN BYPASS TEMP A text is flashing and audible alarm is present ALARM TEMP A PROBE NOTE Text may display Temp B OPEN VENT PROBE text is flashing and audible alarm is present ALARM OPEN BP PROBE Any ALARM text is flashing and an audible alarm is NOT present SOUND THERMISTOR text is flashing and audible alarm is present ALARM THERMISTOR OPEN www custombiogenics com CAUSES Bypass valve disconnected from power source Bypass valve defective Thermocouple probe plug is disconnected Thermocouple probe is damaged Thermocouple probe plug is disconnected Thermocouple probe is damaged There is an alarm condition
35. is menu to select the end date that you want the alarm data to end on the report display or printout 99C jJ Press this button to return to whatever screen was showing when the report was requested Press this button to save the setting and Return to display 4 Main Menu see page 16 www custombiogenics com This display will appear when enter is selected on display 99B see above Use this menu to choose weatehr you want the report to be printed or displayed on the screen COC BS LCD DISPLAY FUNCTIONS Program Mode Display Windows Press this button to return to whatever screen was showing when the report was requested Press this button to scroll to the previous recorded event Press this button to scoll to the next recorded even __ f this to save the setting and Return to display 4 www custombiogenics com Press this button to save changes and advance to screen 47 below Main Menu see page 16 Press this button to advance to screen 47 below Press this to discard changes and return to display 4 MAIN MENU see page 16 This display will appear when DISPLAY is selected on display 99C see page 24 Use this menu to view either the ALARMS or DATA that was selected on screen 99 see 15 This display will appear when OFAF function is set to SIMULTANEOUS or SEQUENTIAL see page 24 display 44 and the
36. its hold LN2 in a jacketed space between the vacuum insulated space and storage chamber it cannot be calibrated the same as S Series storage units Customers have multiple options to choose from in how they want to go about calibrating their Isothermal storage unit For all options below please call for pricing and further details N I S T traceable options Purchase Calibration Equipment Rent Calibration Equipment Have a qualified technician sent to your facility to perform the calibration Return the controller to Custom Biogenic Systems to be calibrated gt 9 t www custombiogenics com 28 CB Ss t TROUBLESHOOTING GUIDE ALARMS CONDITIONS HIGH ALARM text is flashing and audible alarm is present ALARM HIGH ALARM Liquid level in the vessel has risen above the HIGH level set point LOW ALARM text is flashing and audible alarm is present ALARM LOW ALARM Liquid level in the vessel has fallen below the LOW level set point www custombiogenics com CAUSES Check to see if unit is filling If unit is filling If unit is not filling Supply tank is empty Supply tank is shut off Supply tank pressure is too low 3 or more storage units are filling at one time from a single supply line 29 SOLUTIONS Push the STOP button if fill continues the solenoid valve may be frozen open push the FILL and STOP buttons 15 to 20 times to un stick the valve If the fill con
37. jquessy 35 logenics com www customb 2301 Controller Wiring Diagram Part 1 0 2 A C J 4 COM PORT HT OUTPUT INPUT LCD PROGRAM SWITCH S ETHERNET 5 BLUE RED T C LID B P BLUE RED T CLIDA BLUE RED T C VENT IN PROGRESS REMOTE ALARM FILL VENT FILLVALVE BYPASSVALVE COM NC 2AVDC INPU BI SEE eas VENT LID LID ETHERNET RS 485 RS 485 SENSOR FILL VENT TEMPA TEMPB PORT IN OUT PORT IN PROGRESS VALVE OUTLET SOLENOID VENTSOLENOID GLOBAL 24 2 VALVE OUTLET REMOTEALARM VDC WC GND RX CTB TX WV BACKLIGHT EQUENTIAL INPUT SWITCH THERMISTOR INPUT LID LID LN2 LID LN2 LEVEL TEMPB TEMPA LEVEL TEMP m m LE 3 SINE Ss EN NE EE SEQUENTIAL FILL OUTPUT AUX USB PORT 16 PIN CONNECTOR LID SWITCH THERMAL OVERFLOW RS 485 PRINTER PORT SENSOR www custom biogenics com 36 gt 1 H 4 Alquassy 14014 1052 6 8 0 100477777 2 peauueg SS X c 9 8 i 8100 1007 peeuued 5 5 02 7 1 Z i i v103 LO9g7777777777 eseg 19 o 3u0 000 9 i i o i 6600 100377777777777 sHopuels G 0 6 g
38. l appear when LOG is selected on display 7 see page 16 This menu is used to choose how often you wish the data Temp A Temp B and Level to be recorded The recorded info can be displayed printed when the report function is selected from screens 1 2 or 3 COC BS LCD DISPLAY FUNCTIONS Program Mode Display Windows Press this button to Press this button to Press this button to Press this button to disable OFAF and select Simultaneous select Sequential discard changes return to display 74 MAIN MENU see page 16 Press this button to return to whatever screen was showing when the report was requested 99B Press this button to return to whatever screen was showing when the report was requested and Advance to screen 46 see page 25 Cf JC JC JC and Advance to return to display 7A MAIN MENU see page 16 Press this button to discard changes and return to display 4 MAIN MENU see page 16 Press this button to discard changes and return to display 4 MAIN MENU see page 16 This display will appear when OFAF is selected on display 7 see page 16 This display will appear when either alarms or data is selected on display 99 see page 15 Use this menu to select the start date that you want the alarm data to begin on the report display or printout This display will appear when enter is selected on display 99A see above Use th
39. lays possible They are as follows Standard Display Display number 1 page 14 Standard Display While Unit is Filling Display number 2 page 14 Standard Display With Lid Open Display number 3 page 15 Alarm Condition Display Display number 90 page 15 Alarm Condition Display With Audible Alarm Muted Display number 91 page 15 Printing Report Display number 99 page 15 The Alarm Condition display will give the specific cause of each alarm condition that may be occurring The following is a list of the possible alarm conditions Low Alarm Open Fill High Alarm Open Bypass Source Alarm Temp A Probe Temp A High Temp B Probe Temp B High Open BP Probe Thermistor Open LN2 Overflow Optional Additional Equipment Required In the Program Mode there are various displays possible The following is a list of programming functions and the corresponding display windows For each of these programming functions there may be sub menus that follow the primary display window The sub menus are not listed here but are shown and explained following the primary windows The primary programming functions are as follows Main Menu Display number 4 page 16 Provides access to Date Time Settings Display number 8 page 17 Tank ID Settings Display number 13 page 18 Liquid Level Settings Display number 15 page 18 Main Menu 2 Display number 5 page 16 Provides access to Temp Settings Display number 19 page 18 P
40. nd to make changes in programming as shown by the text which appears above the button 4 Second Menu Select Button This is used to select various control menus and to make changes in programming as shown by the text which appears above the button 5 Third Menu Select Button This is used to select various control menus and to make changes in programming as shown by the text which appears above the button 6 Fourth Menu Select Button This is used to select various control menus and to make changes in programming as shown by the text which appears above the button 7 Scroll Down Button Scrolls to lower numbers when programming electronics 8 Scroll Up Button Scrolls to higher numbers when programming electronics 9 Program Lock Key Switch Switches the controller between the Standard Operat ing Mode and the Program Mode Also protects electronic programming from being changed by unauthorized personnel www custombiogenics com 6 C E L5 5 Ol 6 8 ayy He 152 5 ai 0097 1 989 ED v s s90gr anug iajeus 051 3 LL 15 Nua bog aAnpqussqudal ueadoin3 Wav TV 1LOW3MH 1v8019 LALO Lik vusf p 5 0 31 84 WAT 8 dW3 35 035 ENI ZNI
41. ppear on the display and power to all solenoid valves will be disabled www custombiogenics com 11 REMOTE ALARM WIRING Locate the Remote Alarm Connector on the controller 10 on page 7 amp 8 This is where the remote alarm or auto dialer will plug into the control unit REMOTE ALARM CONNECTOR Diagram shows connections for wiring arrangement Normally Normally Closed Open First wires from the remote alarm or auto dialer must be connected to the plug end which plugs into the Remote Alarm Connector Use the diagram to connect wires from the remote unit Remote Alarm connector jack on unit back panel Push into place only goes one way En Remote Alarm connector be sure to push inside pin after wiring When unit goes into an alarm condition or loses power the contacts will switch their Open or Closed state to be the opposite of when there was not an alarm condition thereby sending a signal to the remote alarm or auto dialer Wiring it to common and normally open will trigger the remote contact if a power failure occurs STORAGE UNIT PARAMETERS Cooling medium Liquid nitrogen only supplied by a pressurized cryogenic transport vessel Operating Pressure 22 p s i Power Supply Voltage 120 Volts AC 60 Hz standard 220 Volts AC 60 50 Hz optional Amperage 2 amp max Fuse VAC 2 amp Buss fuse 220 volts slow blow T2A 250V automotive glass tube type fuse Specification 3000
42. r more information When the line reaches this temperature the vessel will begin to fill When the liquid level reaches the high level set point a signal is sent to the controller which deactivates the solenoid valve and stops the flow of liquid into the vessel As long as the unit is powered up this fill sequence repeats as necessary maintaining the desired liquid level Check unit daily to ensure proper operation and safety of the stored samples For the V Series Carousel units it is essential to lift the lid each day and check for vapor and signs of proper freezing www custombiogenics com 4 C E 5 __ OPTIONAL CRYOMONITOR SOFTWARE CryoMonitor software is available to connect your storage unit directly to a PC for full access to the setup and monitoring of the system electronics Pictured below are the SETUP and DATABASE menus of the software Contact Custom BioGenic Systems or your sales representative for more information about the CryoMonitor software 2301 CRYOMONITOR SOFTWARE VERSION 2 0 COMING SOON www custombiogenics com 5 CEB S e FRONT PANEL CONTROLS OFF ON www CustomBioGenics com 1 Key Switch Power Control Main power control for the unit 2 LCD Display Window Text interface for the storage unit electronic settings See page 13 for complete listing of all display menus and functions 3 First Menu Select Button This is used to select various control menus a
43. rinter Functions Display number 22 page 19 Bypass Settings Display number 24 page 19 Main Menu 3 Display number 6 page 16 Provides access to Control Validation Display number 40 page 20 Fill Timer Settings Display number 7A page 17 Software Revision level Display number 42 page 23 Main Menu 4 Display number 7 Page 16 Provides access to One Fill All Fill Display 44 on page 24 Log Display 45 on Page 23 Return to Main Menu Display 4 on page 16 www custombiogenics com 13 gt Se LCD DISPLAY FUNCTIONS Using The Diagrams to Understand the Display Functions WARNING NEVER USE AN OBJECT OTHER THAN YOUR FINGER TO PRESS THESE BUTTONS DOING SO WILL RESULT IN PERMANENT DAMAGE TO THE CONTROLLER OVERLAY Below is a sample display window as they re shown in the following section Use the diagram to become familiar with the way this section is organized and how the information is presented in reference to each display window and it s functions NOTE Tolerance on Liquid Level Display is 1 2 54cm and The Tolerance on the Temperature display is 1 C SAMPLE DISPLAY WINDOW X s ARE USED IN PLACE OF NUMBERS IN THE DISPLAY WINDOW SINCE THE ACTUAL NUMBERS WILL VARY This is the standard display which will appear during normal operation under most circumstances THE TEXT TO THE RIGHT OF THE DISPLAY EXPLAINS THE SPECIFICS OF THE DISPLAY MESSAGE AND THIS NUMBER TO THE LEFT OF EACH WINDO
44. ructions press TEMP 7 Open the manual valve on the supply tank Check for leaks at the connection points then proceed to normal operation OPERATION The V Series Carousel Storage Units are designed to maintain liquid nitrogen levels within specified parameters inside the walls of a cryogenic storage vessel This is achieved by measuring gravitational weight of the liquid nitrogen with a pressure sensing unit in the controller The controller activates the FILL solenoid valve when the liquid level goes below the LOW level set point and deactivates the solenoid valve stopping the fill when the level reaches the HIGH set point The Bypass Function has an additional solenoid valve to vent vapor from the transfer lines before the FILL solenoid is activated In the description below the part of the sequence which is unique to the Bypass Function is in brackets All other information refers to units with or without the Bypass Function During regular operation the sequence of events is as follows When the liquid level in the vessel falls below the low level set point a signal is sent to the controller At this time the FILL solenoid valve is activated allowing the flow of liquid into the vessel The Bypass Function will activate the VENT solenoid first to release gas or vapor from the transfer lines The venting will continue until the line temperature reaches approximately 150 to 160 C This can be adjusted consult the factory fo
45. t i 0s500 100377777777777777 syopueis S 0 J W 7 i 1063 9195 _ Utew 49 04300 LOEZ 700 10057777 777777 M S v e 2 9 C 1003 109977777777777777 2 11043407 0005 1 J9quunw Hed uondu sed Weg LOEZ gt 37 www custombiogenics com tranta lie mn PS DL quuss Sv KMCERICRIE BL eo S C gt 38 www custombiogenics com Hed yue 000 SES i C EB a 39 www custombiogenics com Meg weibeiq jesnoje 0005 CLEANING amp MAINTENANCE Sanitizing and Decontamination The CBS large LN2 storage units are constructed with an inner fabricated entirely from stainless steel Any cleaning solution that does no react stainless steel can be used in the sanitation process of these tanks A mixture of 30 chlorine bleach with 70 water solution is considered the best method for decontamination However any household detergent or mild soap solution is suitable and can be used This includes bleach detergents and mild soaps Other cleaners and disinfectants that can be safely used inclued hydrogen peroxide and denatured alchohol NOTE DO NOT USE ANY PETROLEUM BASED CLEANING SOLUTIONS To per
46. te and time settings NOTE DAY MONTH YEAR gt E lt gt LCD DISPLAY FUNCTIONS Program Mode Display Windows These arrows adjust value 13 This display will appear when TANK 10 15 selected 7 display 4 The Tank ID is necessary when using the CryoMoriltor coimputer Press this button to Press this button to interface software save the setting and discard changes and return to display 13A return to display MASTER SLAVE 13A Master Slave see page 23 see page 23 15 This display will appear when LIQ D LEVEL 15 selected on display 4 Use this menu to select measurement C C preferences for the liquid level settings i e inches or cm Press this button to Press this button to Press this button to select INCHES and select CENTIMETERS discard changes and advance to display 16 and advance to return to display 4 below display 16 MAIN MENU below see page 16 These arrows i TE i display Wen 16 INCHES or CENTIMETERS is selected display 15 Use this menu to select and LOW liquid level settings Press this button to Press this button to Press this button to Press this button to save settings and move cursor to move cursorto discard changes return to display 4 SET LOW SET return to display 4 MAIN MENU MAIN MENU see page 16 see page 16 This display will appear when TEMP 19 is selected
47. tinues turn off the manual valve on the liquid nitrogen supply tank and let the solenoid valve warm to room temperature then re open the manual valve to see if proper operation returns If not the solenoid valve may need to be disassembled for service see page 39 Cleaning and Maintenance There may be an obstruction in the solenoid valve Remove solenoid valve and disassemble for service see page 39 Cleaning and Maintenance Replace supply tank Open manual valve on the supply tank Check pressure in supply tank 25 p s i maximum 20 p s i minimum 22 p s i recommended Storage vessels will fill very slowly and alarm will correct itself gt continued on next page gt E lt gt TROUBLESHOOTING GUIDE ALARMS CONDITIONS CAUSES LOW ALARM text is flashing and audible alarm is present Solenoid valve operating erratically ALARM LOW ALARM The LN level in the vessel has fallen below the pressure sensing port LOW ALARM text is flashing audible alarm is present and level display reads 00 0 cm or 00 0 in ALARM LOW ALARM www custombiogenics com 30 SOLUTIONS Push STOP button briefly and listen for the fill solenoid valve to click This sound is the valve operating If the click is heard and the supply tank still has liquid and pressure in it there may be an obstruction in the valve or in the line If no click is heard
48. to your LN2 supply source Select Simultaneous mode All freezers will begin to fill at the same time until all linked controllers have reached their High Level Set Point simultaneously Custom Biogenic Systems recommends selecting Sequential This mode allows freezers to fill one at a time with a primed and cold supply system making it easier to maintain proper filling pressure and reducing LN2 transfer loss This is more efficient than a keep cold system For best results with large pipeline LN2 supply systems adjustment to the source alarm timer may be required Please contact Custom Biogenic Systems with any questions regarding the function and setup of the SEQ OFAF feature In the event of an alarm status that stops the freezer from reaching the High Level Set Point the 2301 controller will not trigger the next freezer in line to fill However the auto fill function always takes priority over the SEQ OFAF settings SEQUENTIAL FILL SIMULTANEOUS FILL 2 TANK3 4 2 TANK3 4 fra on ome ra HL HL 2 moe LL LL LL _ _ www custombiogenics com CEB S 10 HL LL HL LL HL LL FEATURE DETAILS Fill Timer See page 16 screen 6 Enables setting the timer to fill the freezer at 24 48 or 72 hour intervals at the time selected Note that the Auto Fill setting will always override the Fill
49. ustombiogenics com 9 CES V SERIES CAROUSEL MANUAL FILL INSTRUCTIONS Without power there will be no digital display to give the liquid level and the liquid cannot be seen visually since it is contained inside the walls of the Isothermal Unit Thus the lid must remain open during the manual fill process With this in mind the best way to gauge the fill level is when the tank is nearing maximum fill liquid nitrogen will begin to spray from the vents located near the top edge of the inner walls of the vessel When this is seen the supply valve should be shut off immediately before excessive amounts of liquid nitrogen begin to spill into the storage space VENTS Check unit daily to ensure proper operation and safety of the stored samples For the V Series units it is essential to lift the lid each day and check for vapor and signs of proper freezing www custombiogenics com C E3 lt gt FEATURE DETAILS SEQ OFAF system See page 16 screen 7 SEQUENTIAL MODE recommended Connect all 2301 controllers to your LN2 supply source Select Sequential mode to maintain optimum fill pressure and significantly reduce LN2 transfer loss Once the first controller reaches its High Level Set Point it will activate the next controller This process will continue until all linked controllers have reached their High Level Set Point sequentially SIMULTANEOUS MODE Connect all 2301 controllers

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