Douglas Bags User Guide
Contents
1. UNIVERSITY OF EXETER Douglas Bags User Guide Assembling the mouthpiece e Insert the white valves as shown in the image to ensure that the air flow is correct e Then screw together the mouthpiece and end pieces Vacuuming the bags ami ais Connect the tube to the Douglas bag Open Valve Turn the valve 90 AN clockwise to open the eS valve to the Douglas bag Flick the switch to the on position on the Harvard dry gas meter Turn on the vacuum pump until the counter on the Harvard dry gas meter AR stops increasing A mea You may need to squeeze the bags to ensure all the air is out of them for the best results Connecting breathing tube to Douglas bag Connect breathing tube to the Douglas bag as shown in the image Get the subject to put the mouthpiece in and wear a nose clip To collect air turn the valve 900 and the bag should begin to inflate Once complete close the valve again and if necessary open the valve to the next Douglas bag on the trolley to collect more air Closed valve Open valve Using the Servomex to sample expired alr After completion of the test using the Douglas bags gas collected needs to be analysed for volume and composition The servomex will give you a expired O and CO Connect the sample line from the Servomex to the Douglas bag Turn the red and white valves to the position shown to allow air circulation Star2. t the pump on the Servomex and wait for the O and CO figures to settle Then turn the valves back to their original position Note The colours of the valves correspond to the dot stickers on each side of the trolley Volume analysis Connect the tube from the eme Harvard dry gas meter to the Douglas bag as shown Turn on the Harvard dry gas meter by flicking the switch and press reset to zero the litres screen if not on zero ade _ ey Fa r r y Fi o e as S E E E A Py gt y e i e M E HS a frita A lt S t t E pump and run until the litres reading no longer continues to rise The reading you get is the number of litres of gas used during the test Cleaning All tubes mouthpieces and nose clips need to be sterilised once testing has finished Fill a bowl with warm water and add 2 virkon tablets Soak for 10 minutes and get as much water out of the tube as possible and hang on the rack to dry Put the mouth pieces and nose clips in the drying cabinet
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