OPERATING INSTRUCTIONS
Contents
1. Drying insufficient Extraneous particulates Impurities in tap water Blisters on film 3 Water droplets left on film before drying Reticulation e Tap water temperature too high2. to PUMP ce ect ligne Cy Er ey i Pull out until the holder stops and turn it completely Wait for 30 seconds and 3 remove the holder Align guide pin and push in amp Fig 5 2 11 Specimen holder motion diagrams 5 2 6 Electron Beam Generation 1 Confirm every three months that the reading of the pressure meter on the HT tank is 1 7 or more If less replenisl the tank with gas see the maintenance manual 2 Confirm that the column pressure is lower than 10 exp 4 Pa Note The column pressure is indicated on the meter L2 1O when the vacuum gauge selector L2 7 1s set at PEG Use the lower inner Pa scale when the L lamp on the left side of the meter is lit and the upper outer Pa scale when the H lamp is lit 3 Make sure that the ACCEL VOLTAGE READY lamp L1 3 is lit and the FILAMENT L1 8 is set at OFF 4 Make sure that the dispensing and receiving magazines are in the camera chamber see Sect 5 2 2b and the specimen holder is in the goniometer see Sect 5 2 4 Note When the specimen holder is not in the goniometer election beam generation 1s impossible Specimen n holder Fig 5 2 12 Specimen holder installed in the goniometer 5 Set the accelerating voltage 5a Obtain PAGE 1 on the CRT Note PAGE 1 2 or 61s successively appears on the CRT every time the PAGE key 1s depressed 5b Set the ACCEL VOLTAGE value on the CRT to the desired value with the ACCEL VOLTAGES switch L
3. JEM 3010 HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPE OPERATING INSTRUCTIONS JEM 3010 a 300KV JEOL make High resolution analytical TEM has been born out of the ultra high resolution TEM techniques One can directly carry out Ultra micro area X ray analysis micro area analysis electron diffraction convergent beam electron diffraction while observing structure images which show atomic arrays Thus permitting more accurate structural analysis Operating Panel The operating panel has a minimum number of switches and controls placed in an easy to use layout This is combined with a personal file scheme which together provides much easier operating environment The operating panel is divided into 6 modules 3 on either side of the main column to understand key locations during description of operating procedures namely 1 L1 top left panel Column opearator 2 R1 Top right panel 3 L2 middle left panel Column opearator 4 R2 bottom right panel 5 L3 Bottom Left panel Column opearator 6 R3 Bottom right panel Please see the above figure During description each button switch will be referred according to the panel in which it is located For example Lens power supply switches L3 2 and L3 3 means switches 2 and 3 on left bottom panel 3 Name of these switches are also written on the panels Panel L 1 i Ed DTE STEP SELECTOR SAFETY PRECAUTIONS For the proper use of the instrument please read fol
4. 18 deg C 5 While waiting make a note of number of Unused films on the CRT R 1 If unused films are 0 then load fresh set of films from the desiccator 5 2 2 Film Loading 5 2 2a Loading films into the dispensing magazine 1 Remove the lid from the dispensing magazine and fully depress the bottom plate until it is clamped Note This procedure should be carried out under a safelight in a dark room 2 Insert an unexposed film into each cassette with the emulsion side facing up Fig 5 2 1 3 Place the loaded cassettes 1n the dispensing magazine and replace the magazine lid Up to 50 cassettes can be loaded in one magazine Note Do not mistake the dispensing magazine for the receiving magazine see Fig 5 2 2 Emulsion Dispensing magazine Fig 5 2 1 Loading films into the dispensing magazine A Rvs Syne um SE S EE E T V MASS oe M mee 5 MEUS p Dispensing magazine Receiving magazine Fig 5 2 2 Magazines 5 2 2b Inserting or removing the magazines into or from the camera chamber 1 Make sure there is no unused film in the camera chamber The number of unused films is indicated after UNUSED on PAGE 1 Fig 5 2 3 displayed on the CRT R1 14 That is 1f the number decreases to 2 1 and then returns to 50 there is no unused film in the camera chamber If PAGE 1 is not being displayed on the CRT make the CRT display PAGE 1 in accordance with Sect 5 2 9 P A NO PA SmE sec A We OSO
5. Do not allow the illumination to become too bright with the PROJ switch L3 1 off otherwise the fluorescent screen may burn due to excessive electron bombardment 19 Perform the condenser lens alignment as follows a Set the magnification to 50 000 times and obtain the smallest beam spot with the BRIGHTNESS knob b Set the spot size value to TEMI 3 c Turn on the DEFLECTOR GUN switch R2 1 and center the beam spot with the SHIFT knobs R2 2 d Set the spot size value to TEM4 3 e Center the beam spot with the left and right SHIFT knobs L1 17 R1 1 f Repeat Steps 19b through 19e until the beam spots with the spot sizes of TEM 1 3 and 4 3 remain at the screen center 5 2 7 Inserting the Condenser Aperture into the Beam Path 1 Depress the MAG2 switch R1 3 manipulate the BRIGHTNESS knob L1 14 so as to obtain the smallest illumination spot and bring the converged illumination spot to the screen center with the SHIFT X and Y knobs L1 17 R1 1 2 Select the desired aperture size with knob 1 Fig 5 2 14 3 Note Decreasing the aperture size improves the image quality but darkens the image Dot Knob 1 Knob 2 Knob 3 Fig 5 2 14 Condenser aperture assembly Gradually turn the BRIGHTNESS knob L1 14 clockwise If the illumination spot center deviates from the screen center Fig 5 2 15a return the spot to the screen center by manipulating knobs 2 and 3 Manipulate knobs 2 and 3 so that the ill
6. decreases to 150 j1A or less if it 1s higher than 150uA The valve functioning is displayed on the diagram L2 13 3 The PUMP AIR switch setting cannot be performed unless the switch 1s pulled 3 When the green lamp lights up 1 e the goniometer evacuation 1s complete slightly turn the holder clockwise and push it in and further turn it clockwise and push it in completely n ESE SSR DOS ESSEC ERE SSSI ERE A CAUTION mm Be careful not to pinch your fingers between the specimen holder and goniometer e Always hold the specimen holder while the holder is being inserted into the goniometer If you do not do so the specimen holder may hit the goniometer causing damage to the goniometer due to the force of yacuum Guide groove Guide hole Goniometer Guide Pin eS Specimen holder Guide rod Fig 5 2 10 Specimen holder insertion 5 2 5 Removing the Specimen Holder from the Microscope Column 1 Set the FILAMENT L1 7 to OFF 2 Open the nitrogen gas source valve 3 Pull the specimen holder turn it counterclockwise pull it turn it again counterclockwise completely 4 Set the goniometer PUMP AIR switch Fig 4 1 2 to AIR wait for 30 seconds and remove the holder Holder insertion Holder removal Push in completely Pull out completely puncompeeyc c oe T ee lan ye m urn completely CEN Set the switch Set the switch 7 PARC Tu ompletely m completely cea
7. placed correctly the specimen grid will not be secured 6 Raise the cartridge securing jaw with the cartridge removing tool Fig 5 2 7 7 Place the specimen cartridge end under the cartridge securing jaw so that the guide pin under the jaw is inserted in the cartridge guide hole Fig 5 2 7 And make sure that the cartridge 1s tightly secured with the jaw there should be no gap between the jaw and cartridge Specimen securing nut BE oO Guide hole Washer 4 7 Specimen cartridge Fig 5 2 9 Specimen loading 5 2 4 Inserting the Specimen Holder in the Microscope Column 1 Make sure that there is no dust and or lint on the specimen holder O ring 2 Align the specimen holder guide pin with the guide groove on the microscope column Or goniometer push the holder in the goniometer until it stops and set the goniometer PUMP AIR switch Fig 4 1 3 to PUMP The yellow lamp lights up and the goniometer evacuation commences Notes 1 Do not turn the holder while carrying out this step 2 The goniometer evacuation will not be performed in the fallowing cases When values Vi and V2 are closed When the P15 reading the reading on the meter L2 1O when the vacuum gauge selector L2 7 is set at P15 is higher than 150 aA with valve V13 open To perform the goniometer evacuation wait until valves Vi and V2 are open if closed and wait until the P15 reading
8. 0 88 10 105 10 7 Set the largest condenser aperture into the electron beam path and retract the high contrast objective aperture and field limiting aperture from the path as follows Condenser aperture Set the 2nd largest dot on knob 1 to the dot on the assembly refer to Sect 4 1 High contrast objective and field limiting apertures Knob 3 Knob Fig 5 2 13 Aperture assembly 8 Confirm that viewing chamber isolation valve V2 is open If the large fluorescent screen is raised lower it by depressing the SCREEN switch R1 11 A diagram indicating which valve 1s open on control panel L2 Note If V2 1s closed the electron beam cannot be generated 9 Gradually turn the FILAMENT knob L1 8 to the knob stopper and adjust the BIAS MODE COARSE and FINE switches L1 1U so that the beam current is about 5 uA Note The beam current is the difference between the BEAM CURRENT reading when the FILAMENT knob LI 8 is at the stopper position and that when the knob is at OFF 10 Darken the room and adjust the control panel illumination with the PANEL LIGHT knob LI 9 11 Obtain the spot size value of TEM2 3 lla Let the CRT display PAGE 1 11b Turn on the TEM LI 19 1 le set the SPOT SIZE value on PAGE 1 to TEM2 3 with the SPOT SIZE switch L1 12 and a SELECTOR knob LI 23 12 Make sure that all the LENS switches L3 1 are set at ON and depress the MAG2 switch R1 3 The magnification indicated on t
9. I 4 6 Turn on built in lamp lights up the HT switch L1 5 and confirm that the BEAM CURRENT meter Li 6 reading detecting current becomes stable in the current range shown in Table 5 1 If the BEAM CURRENT meter reading does not become stable carry out the following Steps Note The ACCEL VOLTAGE READY lamp LI 3 flickers during accelerating voltage increment 6a Make sure that the FILAMENT READY lamp L1 7 1s lit and depress the accelerating voltage to the lowest level and slowly turn approximately 1 graduation per 30 seconds the FLLAMENT knob L1 8 to the stopper position The BEAM CURRENT meter reading increases Note The FILAMENT READY lamp L1 7 does not light up when the specimen holder is out of the microscope column goniometer 6b After the BEAM CURRENT meter reading becomes stable set the FILAMENT knob to OFF increase the accelerating voltage by 20 kV and set the FILAMENT knob L1 8 to the stopper position in the same way 6c Repeat Step 6b above until the original accelerating voltage 1s obtained Note If the BEAM CURRENT reading becomes excessively high the supply of high voltage power is automatically turned off and the BEAM CURRENT reading be comes 0 In such case reduce the accelerating voltage to the next lower value and turn on the HT switch Table 5 1 High voltage and related detecting current values High voltage kV Detecting current LA 100 35 10 150 53 1096 200 70 10 25
10. M NAG Unused Text No sShecitmen Tilt Focus DU n a7 A NX i B dd Fig 5 2 3 PAGE 1 2 Make sure the FILAMENT knob L1 8 is set at OFF and the PHOTO switch lamp R1 8 is not lit If the PHOTO switch lamp is on turn off the FILM ADVANCE AUTO switch R1 11 and depress the PHOTO switch 3 Open the nitrogen gas source valve and turn the camera chamber door handle Fig 5 2 4 clockwise until it stops about 90deg Camera chamber door Door handle Fig 5 2 4 Camera chamber 4 Open the camera chamber door and close the nitrogen source valve If the door does not open in five minutes after turning the door handle clockwise turn the handle fully counterclockwise then turn the handle clockwise again and wait for another five minutes Repeat this turning and waiting until the door opens Notes 1 If the gas source valve is not open and or no nitrogen gas is stored the camera Chamber door does not open 2 Carry out Steps 5 to 9 as quickly as possible so as to avoid exposing the camera Chamber to the atmosphere longer than absolutely necessary 5 Draw out the magazine stand by pulling the handle Fig 5 2 5 CAUTION Do not touch the camera drive mechanism in the camera chamber 6 If there is an empty dispensing magazine in the magazine stand remove it by lifting it out Then place the dispensing magazine loaded with unexposed films squarely in the magazine stand Notes 1 If the magazine is not p
11. a few films are to be processed developing trays may be used However if many films are to be processed at one time it is recommended to use suitable tanks and hangers stainless steel tanks polyvinyl chloride tanks etc are the best Immerse the film in a stop bath 2 to 3 glacial acetic acid solution 18 to 21 C and leave it in for approx 30 seconds This is to suspend development so as to prevent the film from becoming blotchy and to prolong the effectiveness of the fixer Immerse the film in a rapid acid hardening fixer 18 to 21 C and leave it in for approximately ten minutes the fixing time should be at least two or three times the time it takes for the negative to clear This 1s to dissolve the photosensitive silver halide white part of the film and thereby make the unexposed part of the film transparent Thereafter processing can be carried out under an ordinary light T 8 9 Hanger Film Develop gt Stop c Fix c Wash gt Finalnnse c Dry wet Fig 5 2 25 Film processing Wash the film in running water 15 to 2000 for 30 to 60 minutes This removes the complex salt and fixing solution The washing time can be considerably reduced by immersing the film in a rinse accelerator prior to washing it in running water Immerse the film in a final rinse bath weak solution of anionic surfactant 18 to 21 C and leave it in for approx 30 seconds or wipe both surfaces of the film carefully with a so
12. age adjust the Z CONT switch Fig 3 3 1 so as to obtain a single stationary image If the image is underfocused or overfocused it will appear as a double 1mage as shown in Fig 5 2 22 f Turn off the WOBBLER IMAGE X or IMAGE Y switch R1 5 IMAGE X or Y switch im u D L SERM m ipe Peat NAA aa a NM eut wt vn Under focus iiec IN SSSA A X SENSORS SN SN RAS NM hs ROMAN A a E EAD rd PANAN N SRS x NS 1 ASA NN EE S REO RC SISO RIS In focus nOn k Rater aea NA GA dagte e ANA RN AA INN PRU MAE v QE SES RO UNES 3 ones SAS SECS RONEN SE SENS NUS ie AN ej TA S O V I S 3 NS XN S N s Ni e Nd E US e 0O cus 3 A AS SES M SNAM RENS S SONUS aE PN A RANE AW NS UR ah y me ii AN Ah EN a A a AE ARAS T s uev x ANN DS APPIAN i Rea Ay m ARTS aes ERA NY SO LAN AVES E ERN SA E ane Seat M Salata Qa Pii SET MAN ES eT ERN b enn SEE eR EMEN RV NEAR 2 Rae SERERE ADR T a Aone eat at Fig 5 2 22 Focusing with the image wobbler 5 Adjust the image brightness a Obtain PAGE 4 on the CRT b Adjust the BRIGHTNESS knob L1 14 so that the exposure time displayed on the EXPTime line becomes the desired value usually 2 to 4 sec Exposure time Shecinen Tilt Focus TO Fig 5 2 23 Exposure time 6 Depress the PHOTO switch R1 8 with the small screen in the beam path and after the built in lamp of the switch lights up depr
13. e number of unused films see Sect 5 2 11 d Depress the 4 key Receiving magazine Dispensing magazine Magazine stand Magazine stand handle Fig 5 2 5 Magazine stand Acme sec r1 Fig 5 2 6 Writing the UNUSED number 5 2 3 Loading the Specimen Holder with a Specimen 1 Remove the specimen holder from the microscope column see Sect 5 2 5 2 Insert the cartridge removing tool into the cartridge securing jaw raise the jaw with the tool and remove the specimen cartridge see Fig 5 2 7 3 Use the specimen loading tool to unscrew the specimen securing nut remove the washer and specimen see Fig 5 2 8 Unscrew the nut after aligning the claws on the tool with the cuts on the nut Cartridge removing tool OC7WX Specimen holder 4 RE mE MEE securing Jaw SS Guide pin ee Guide hole e Specimen cartridge Fig 5 2 7 Cartridge removing Specimen loading tool Specimen securing nut Specimen securing Qc D S NWasher t e i CD Cartridge Specimen Fig 5 2 8 Specimen loading 4 Place a specimen grid with the specimen facing upward in the specimen cartridge and the washer on the specimen Fig 5 2 9 l Secure the specimen grid with the specimen securing nut using this specimen loading tool That 1s place the nut on the washer insert tool claws in the nut and screw the nut with the tool Note If the specimen grid and washer are not
14. ecial films Fluorescent screen Photographing area This length corresponds to the length of 5 cm on the film Fig 5 2 19 Photographing area 3 Write the information to be recorded on the film on PAGE 1 2 characters can be written on the FILM NO line and 18 characters on the TEXT line Fig 5 2 20 PAGE 1 eS OK lt e ie aR KAY ce am sec m 3016 4 information writing area E t o 0u01 Sh ecimen Ye Tilt Focus DU Fig 5 2 20 Information writing area 4 Focus the image a Push the fluorescent screen lever Fig 5 2 21 until it stops The small Haar Patras tite 7 P 25 is EAT dip a D z T 7 261175471 s ria 2 f f SS s Eyepieces Binoculars Focusing knob Bodytubes Fluorescent screen lever b e Fig 5 2 21 Binoculars c Focus the binoculars on the small fluorescent screen Note First focus the whole binoculars with the focusing knobs and adjust if necessary the intraocular distance by changing the distance between the bodytubes and focus the eyepieces by turning the knurled ring Fig 5 2 21 d Turn on the WOBBLER IMAGE X or IMAGE Y switch R1 5 If the illumination spot oscillates or divides into two spots manipulate the OBJ FOCUS R1 4 to obtain a single stable illumination spot e Observe an image with clear contours on the small fluorescent screen through the binoculars If the image appears as a double im
15. en into the beam path see Sects 5 2 3 and 5 2 4 and depress the DIFF switch R1 3 Keep the electron beam sufficiently spread with the BRIGHTNBSS knob L1 14 2 Obtain a caustic spot zero magnification spot with the DIFF FOCUS knob R1 6 as shown in Fig 5 2 17 If the caustic spot is off the screen center turn on the DEFLECTOR PROJ switch R2 1 and center the spot with the SHIFT knobs R2 2 Turn off the DEFLECTOR PROJswitch R2 1 after centering Fig 5 2 17 Caustic spot 3 Obtain the smallest illumination spot with the BRIGHTNESS knob L1 14 and make the illumination spot coincide with the screen center by manipulating the SH FT X and Y knobs L1 17 R1 1 4 Manipulate aperture assembly knob 1 Fig 4 1 3 to insert a desired objective aperture into the beam path Focus the aperture image shadow with the DIFF FOCUS knob R1 6 6 Manipulate assembly s knob 2 and 3 so that the caustic spot lies in the center of the aperture hole image as shown in Fig 5 2 18 A Fig 5 2 18 Objective aperture alignment 5 2 10 Image Recording by Automatic Exposure 1 Turn on the SHUTTER AUTO switch R1 12 and turn off the FILM ADVANCE AUTO switch R1 11 2 Bring the field of view to be photographed into a photographing area frame on the fluorescent screen with the trackball as shown in Fig 5 2 19 Note A large frame and a small frame are drawn on the fluorescent screen The large frame is used for photography using sp
16. ess the PHOTO switch again Photographing is carried out If the built in lamp is already on depress the PHOTO switch only once Notes 1 The EXP lamp R1 9 lights up and remains lit while the shutter 1s open The built in lamp of the PHOTO switch R1 8 goes out when the exposed film is advanced from the exposing position 2 The PHOTO switch lamp does not light when all loaded films have been exposed 3 The exposure time is determined by an amount of entire electrons either on the small or large fluorescent screen Therefore removing the small screen out of beam path when the large screen is entirely illuminated does not affect the correct exposure time determination 5 2 11 Film Processing 1 2 3 4 5 6 Unload the receiving magazine from the camera chamber see Sect 5 2 2b Adjourn to a darkroom and remove the lid from the receiving magazine and the cassettes from the magazine under a safelight red lamp Carefully remove the film from the cassette Fig 5 2 24 arm HH AA M A M Cassette Se a ye en a Fig 5 2 24 Removing the film from the cassette Immerse the film in a developer 20 0 5 C and leave it in until the exposed latent image becomes sufficiently visible usually three or four minutes Fig 5 2 25 During the developing process agitate the developer or move the film in order to avoid developing marks Note If only
17. ft sponge By so doing film drying time is reduced and film blisters are prevented Dry the film in a drying cabinet the cabinet need not be large or dry it naturally by hanging it in a well ventilated dust free place away from direct sunlight Store the dried film in a negative bag polyethylene cellophane etc and keep the bag in a dry place away from direct sunlight Note Consequences of faulty film processing etc are listed in Table 5 2 assuming that the exposure 1s in order Table 5 2 Consequence of faulty film processing Appearance Possible cause Film inserted into cassette No picture upside down Complete blackening Magazine lid inadvertently opened while being carried e Fogging Faulty safelight Film processing date has expired gt High a IU C MES Developer temperature too high e Low density EINEN CS C EE Developer temperatiir too low Too grainy N Developing time too long Uneven density NNNM MN Developing time too short Loo Effete developer e Spotty staining mottled n Developer agitation insufficient Film electrostatically charged before developing e Scratches streaks e Film emulsion surface rubbed Discoloration of film Fixing and rinsing insufficient during storage Moldy film Method and place of storage unsuitable
18. he MAG line on PAGE 1 is now set at the specific magnification 5 000 times 13 Turn the BRIGHTNESS knob Li 14 When the screen is illuminated by the electron beam proceed to Step 19 If the fluorescent screen is not illuminated carry out the following steps 14 Turn on the DEFLECTOR COND switch R2 1 push the N switch R2 10 then turn off the PROJ switch L3 1 and turn the BRIGHTNESS knob L1 14 If no illumination is observed on the screen proceed to Step 15 When illumination appears on the screen manipulate the SHIFT X and Y knobs L1 17 R1 1 and BRIGHTNESS knob for brighter illumination the turn on the PROJ switch and achieve brightest illumination with the above mentioned knobs Then proceed to Step 19 15 Turn on the DEFLECTOR GUN and WOBBLER GUN switches R2 1 9 and manipulate the BRIGHTNESS knob L1 14 so that the fluorescent screen 1s illuminated by the electron beam 16 Manipulate the SHIFT Y and DEF Y knobs R2 2 3 and the BRIGHTNESS knob L1 14 so as to obtain the brightest illumination at the screen center 17 Turn off the WOBBLER GUN and DEFLECTOR GUN switches R2 1 9 18 Manipulate the SHIFT X and DEF X knobs R2 2 3 and the BRIGHTNESS knob L1 14 So as to obtain the brightest illumination If the illumination becomes gloomy in the course of this operation stop manipulating the knobs turn on the PROJ switch L3 1 and manipulate the knobs again so as to obtain the brightest illumination Note
19. laced squarely in the magazine stand it will be impossible to insert the magazine stand smoothly 2 Two dispensing magazines and two receiving magazines Fig 5 2 2 are provided in order to enhance throughput That is to say while one dispensing magazine is being used the other can be kept in the desiccator optionally available so that the loaded films are ready and demoisturized when needed Also as one receiving magazine is being filled during the course of photography the second one can be Icept handily ready for the next sequence of filming 3 When handling the magazines hold them so as to prevent the magazine lid from dropping out 7 If there is a receiving magazine loaded with exposed films in the magazine stand remove it by lifting it out and replace it with an empty receiving magazine Note Never remove the lid from a loaded magazine outside a dark room 8 Push the magazine stand fully in 9 Close the camera chamber door and while holding it closed turn the door handle counter clockwise as far as it will go Note Before closing the camera chamber door check the O ring and its contact surfaces for dust lint etc A dirty O ring may adversely affect the camera chamber vacuum 10 Write the number of unused films the number of films loaded in the dispensing magazine on the CRT a Let the CRT display PAGE i see Sect 5 2 11 b Enter FUN 4 through the keyboard c Set the UNUSED number Fig 5 2 6 to accord with th
20. lay PAGE 3 and confirm that the name of the objective polepiece being used is displayed on PAGE 3 If the displayed name is different from that of the pole piece being used carry out Sect 5 2 12 Note If the objective polepiece name displayed on PAGE 3 is different from the name of the polepiece being used the correct magnification is not displayed Select the desired magnification with the SELECTOR switch R1 7 a suitable magnification less than 50 000 times and adjust the image brightness width the BRIGHTNESS knob L1 14 If the illumination spot center does not coincide with the screen center center the illumination spot with the SHIFT X and Y knobs L1 17 R1 1 Note If a magnification is selected with the MAG2 switch R1 3 depressed the selected magnification is not stored in the memory However if a magnification 1s selected with the MAGI switch R1 3 depressed the selected magnification is not stored in the memory That is to say the magnification is automatically set to the stored value by depressing the MAGI switch On the other hand by depressing the MAG2 switch the magnification is always set to a specific magnification Sect 5 2 12 User s Comment PAGE 3 Magnification MAG SeK TEM 360 0KU P URP3B Objective F Filament Lag Film Fuji polepiece name SPecimen OQ O RUN Comment Fiq 5 2 16 PAGE 3 5 2 9 Inserting the High Contrast Objective Aperture into the Beam Path 1 Insert a specim
21. lowing safety precautions prior to starting operation e Do not remove the grounding wire Improper grounding may cause electrical shock e Do not mount on the operating table and or console Do not dismount and or reassemble the microscope column The column dismounting and reassembling is allowed to only Jeol Engineer Do not touch the parts with safety labels on them Be careful not to pinch your fingers between the specimen holder and goniometer when inserting the holder into the goniometer Do not put your hand between the upper and lower electron gun chambers while the electron gun lift is functioning Please read the JEM 3010 instruction manual available with EMU completely before actually operating the instrument 5 2 1 START UP PROCEDURE l Make sure that a The Lens Power supply switches L3 2 and Lens L3 1 are set at ON b The Gun air and column air switches L2 8 and 9 are off the built lamps are dim The compressed air pressure is 0 35 to 0 45 MPa gauge pressure The Nitrogen cylinder main valve is tuned On and pressure is around 1 bar e The UNA switch is set at OFF eo 2 Turn ON the main power switch on the distribution board 3 Turn the POWER switch Key clockwise to START via ON hold it at START position for five seconds then release the key and wait for the ACCEL VOLTAGE READY lamp L1 3 to light up 4 Make sure water chiller is running and the temperatures are same as set temperatures
22. umination spot concentrically converges on and spreads from the screen center Fig 5 2 15b when the BRIGHTNESS knob L1 14 1s turned clockwise and counterclockwise around the position for the smallest illumination spot a Incorrectly aligned b Correctly aligned Fig 5 2 15 Condenser aperture alignment 5 2 8 oS Sn Image Observation Turn on the TEM switch L1 19 to obtain an illumination mode of TEM and set the spot size value displayed on the second line on PAGE 1 to TEM2 3 with the SPOT SIZE switch L1 12 and a SELECTOR knob L1 23 Set the magnification to 50 000 times and converge the electron beam with the BRIGHTNESS knob L1 14 Turn on the WOBBLER IMAGE X or Y switch R1 5 The electron beam may be divided into two Make the divided beam single with the OBJ FOCUS knob R1 4 Diverge the electron beam with the BRIGHTNESS knob L1 14 and obtain a single stationary beam if the image has been doubled with the Z CONT switch Fig 3 3 1 Turn off the WOBBLER IMAGE X and Y switches R1 5 Select the desired field of view with the trackball SC 4 or X Y switches SC 3 Notes 1 The selected field of view is indicated by a square mark on PAGE 2 on the CRT see Sect 4 3 2 If LIMIT OVER appears on the CRT bottom with a weak peep sound and specimen shifting 1s halted move the specimen in the opposite direction depress the ESC key KB 1 to erase the LIMIT OVER on the CRT Let the CRT disp
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