Sinar ™ GermPro USER MANUAL
Contents
1. Sinar GermPro USER MANUAL Serial no Purchase Date Date registration card mailed Rev 4 30 11 09 1 0 2 0 3 0 4 0 5 0 6 0 CONTENTS Introduction Reagent GermPro Set up To commence a test Determination General use and maintenance 1 0 2 0 Introduction The purpose of the Sinar GermPro is to measure the ENZYMIC activity in the germs or embryos of grain kernels and seeds This manual details the actions required to determine the germination capacity of samples using a Sinar GermPro In the Sinar GermPro a solution containing Tetrazolium salt is used as a reagent This liquid is sensitive to Hydrogen Creating enzymes The hydrogen produced by the action of these enzymes will reduce Tetrazolium to a light red colour RED FORMALAZIN when a germ capable of germination is in contact with the solution If the seed is incapable of germination no colour is produced Please note if pale stains are present with a slight brown colour this may indicate heat damage and the 4mIGE test may give a low result for the first 2 days as the vigour of the seed may be reduced as it deteriorates It is important that when the reaction takes place a vacuum is created to accelerate the process hence the hand pump supplied with each GermPro As a result tests take only a few minutes instead of several hours normally required by conventional methods Reagent For testing the germinative capacity of malt 235 trip2. a sharp blade carefully split the individual grains lengthways to expose the germ embryo When 100 grains have been split discard half the split grains Place the remaining grains into the incubation slides provided Locate the slides onto the hook on the underside of the stopper Lower the slides into the chamber and secure the stopper Using the hand vacuum pump press the handle several times to evacuate to desired vacuum Normally 20 30 cm Hg Press the RUN STOP key The GermPro will automatically use the stored elapse time The count down time is displayed in minutes and seconds On completion the unit beeps and the word END is displayed To interrupt a test press the RUN STOP key again The unit beeps and the word END is displayed Release the vacuum by squeezing the release lever on the hand pump and remove the stopper with slides for visual inspection The level of liquid with the GermPro should be checked and topped up if necessary Change the Tetrazolium solution on a regular basis to ensure that it remains clean and effective A dirty solution will become tired and less active resulting in poor results The GermPro remains on in standby mode until the next test is to be performed Repeat stages 4 1 4 9 5 0 5 1 5 2 5 3 6 0 6 1 6 2 6 3 6 4 Determination Determine the germination percentage by close observation of the germs in the half grains This should be done within 10
3. henyl tetrazolium chloride is required You will need to use a 0 5 to 1 solution for testing purposes i e 0 5gm 1 0gm to 100ml of distilled water When preparing the solution warm the distilled water without heating This will make the preparation of the solution easier Once prepared store the solution in a dark bottle in the absence of light Shelf life is 2 to 3 months when stored correctly 3 0 3 1 3 2 3 3 3 4 3 5 3 6 3 7 3 8 GermPro Setup Connect the GermPro to a suitable power supply using the power cord supplied Switch on using the switch on rear panel the word HI will appear on the display To set the test temperature press the TEMP key The current temperature setting is displayed Use the UP and DOWN keys to select the required temperature within the range of ambient to 60 C The temperature indexes in 1 intervals Typically a temperature 40 C is required for repeatable accurate results Press the SET key to store the selected temperature The display will flash and the unit will beep to indicate the value has been stored It is not necessary to repeat this procedure again unless a change of temperature is required Use a digital temperature probe to confirm the solution temperature Pour the solution into the glass flask up to the max fill line Place the flask in the heating chamber within the instrument Allow the fluid to warm for approx 20mins Place the temperatu
4. minutes of taking the sample out of the GermPro leaving the sample too long may invalidate the test It is important to remove the sample holder cover and closely examine each half grain with tweezers and use a strong magnifying glass if pre germination is suspected The germination percentage is determined by counting the number of dead pre germinated and heat damaged grains and subtracting it from 100 Live Grains are acceptable when the embryo is stained pink report as Germ Capacity Ensure that cutting damage is not misinterpreted as being a fail General use and maintenance Take care not to overfill the incubation chamber It is recommended that the Tetrazolium solution be changed on a regular basis To clean the chamber rinse with distilled water Leave the GermPro switched on between tests The keeps the dry block heater permanently at the required test temperature Periodically lubricate the orange stopper O ring with silicone grease
5. re probe in the solution Increase or decrease the instrument set temperature as described above to achieve the required solution temperature To set the test time period press the TIME key The current time setting is displayed Use the UP and DOWN keys to select the required time The time indexes in 10 second intervals Press the SET key to store the selected time The display will flash and the unit will beep to indicate the value has been stored It is not necessary to repeat this procedure again unless a change of time is required Remove the vessel stopper it has a domed top with grain slides Unscrew and remove the fluted ring securing the incubation chamber Withdrawer the glass incubation chamber and stand to one side Pour the required Tetrazolium solution into the incubation chamber Fill to the black fill line marked on the side of the vessel Put the incubation chamber back into the GermPro Screw down the fluted ring securing the incubation chamber Replace the stopper with slides A pre heat period is required for the incubation chamber to reach equilibrium This will vary with the selected temperature Allow 30 minutes for a set temperature of 40 C 4 0 4 1 4 2 4 3 4 4 4 5 4 6 4 7 4 8 4 9 5 0 To commence a test Ensure the time period and temperature settings are correct Prepare the grain sample to be tested Select a small quantity of well blended grain Using
Download Pdf Manuals
Related Search