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Digital Sonifier® Models 250 & 450 User's Manual

1. Select keys LCD Display L and e Arrow keys ere Baigrapli Front eee D Amplitude Control Panel Sele Keypad M Power Switch 100 214 239 Rev A 2 1 Introduction to the Digital Sonifier BRANSON 2 2 The 250 and 450 Digital Sonifiers differ only in their output power ratings measured when the output control 15 at maximum setting The 250 maximum available output power is up to 200 watts the 450 s maximum available output power 1s up to 400 watts The digital controls on the Digital Sonifier allow for accuracy and repeatability of control set tings You enter application parameters into the system either through the keypad on the front panel of the unit or remotely through a computer keyboard and can save up to twenty 20 sets of preset control parameters You can view operating parameters through the twenty char acter four line LCD display on the unit control panel and on a remote terminal if one is con nected At System Setup you can select a language for the display English German French Italian or Spanish The Digital Sonifier s power supply converts AC line voltage to 20kHz electrical energy This high frequency electrical energy is fed to a converter where 1t is converted to mechanical vibra tions The heart of the converter 15 a lead zirconate titanate electrostrictive element which when subjected to an alternating voltage expands and contracts The converter vibr
2. 1 2 4 1 setup procedure nenas 4 6 ShipiTiGnit osi erecti utente RE EA 1 3 solids disrupting ER A 3 solution using glass powders with A 4 soundproof box eene eene 4 5 specifications performance ecce ciere ne es 5 2 requiremetit 5 e emere 5 1 standard 4 2 start and stop 4 10 5 De 6 2 sterlizing oet ee dtt tte ete tee idis A 3 SUPPOR 1 1 intended use 1 1 system block 4 2 System component 4 2 system modes entere nnne 6 1 System setup baud rate Saisie t een ats 6 17 date e e ed c ep tiie E ed 6 15 F laTe UC Lo ERE EN 6 9 model eye enr ee tcx eas 6 21 jg p EE 6 5 ipo ES 6 7 EE 6 19 tempetat re rete 6 11 time of day eie ete Cette lere eet 6 13 system setup 6 3 SyStemrstales 6 2 100 214 2
3. 7 Digital Sonifier unit Horn Converter and any special equipment Special information about the Horns is etched into the horn 8 Place the filled in form in a safe place for future reference If you ate using the Printer option you may wish to print a report of the results and keep it on file with the form 100 214 239 Rev A 5 3 Technical Specifications BRANSON Branson Digital Sonifier Setup Form Make a copy of this form and use it to record a benchmark for your system s setup Date Operator Experiment Solution Digital Sonifier Model 250 450 117 Volt 200 245 Volt Unit Serial Number Converter Serial Number Horn Type Parameters Mode Preset Amplitude setting LCD read out Bargraph reading Power Reading watts Other Setup Notes 5 4 100 214 239 Rev A Digital Sonifier Setup and Operation 6 Operation 61 Setup and Operation 6 2 6 2 Front Panel Controls 6 5 6 3 Entering System Setup Parameters 6 9 System Setup Language 6 10 System Setup Date 6 12 System Setup Time of Day 6 14 System Setup Temperature Units 6 16 System Setup Printer 6 18 System Setup Printer Model 6 20 System Setup Reports 6 22 6 4 Entering Operati
4. 100 Press the Setup key to exit the System Setup menu The Setup LED extinguishes 100 214 239 Rev A 6 17 BRANSON Operation System Setup Printer The Printer parameter enables or disables the ability to support a Printer Take this action To get this result PRINTER OFF REMOTE OFF Press the Setup key to display the System Setup menu Its LED lights LANGUAGE ENGLISH TEMPERATURE F 100 PRINTER OFF Use the Up and Down arrow keys if REMOTE OFF necessary to display the line showing LANGUAGE ENGLISH the Printer parameter TEMPERATURE F 100 PRINTER OFF Press the Line Select key that corresponds REMOTE OFF to Printer LANGUAGE ENGLISH TEMPERATURE 100 6 18 100 214 239 Rev A Digital Sonifier Entering System Setup Parameters Press the same Line Select key to toggle between the two possible values for Printer On if a printer will be enabled or Off if it will be disabled PRINTER OFF REMOTE OFF LANGUAGE ENGLISH TEMPERATURE 100 Press the Enter key to store the value for Printer PRINTER OFF REMOTE OFF LANGUAGE ENGLISH TEMPERATURE 100 Press the Setup key to exit the System Setup menu The Setup LED extinguishes 100 214 239 Rev A 6 19 BRANSON Operation System Setup Printer Model The Model parameter selects the printer model from several c
5. Section 6 8 Displays During tbe Experiment on page 6 44 6 6 100 214 239 Rev A Digital Sonifier Front Panel Controls Figure 6 1 Digital Sonifier front panel showing a Ready mode display including Amplitude BRANSON Digital Sonifier PRESET ID __ hr m s MAX TEMP C AMPLITUDE 17 96 s 5 Print Start CAUTION Do not use a sharp or pointed object to press the front panel controls The soft touch mem brane front panel can be permanently damaged 100 214 239 Rev A 6 7 Operation BRANSON 6 2 5 Menu Navigation When the parameter selection menu 15 displayed you can use the Page Up and Page Down arrows to navigate up and down between the pages of the menu to locate the parameters you want to change If there are more menu options above the page currently displayed the Page Up arrow LED 1s active If there are menu options below the current page the Page Down arrow LED 1s active Select buttons when their parameters are available will have their LED lit The Feature buttons and Arrow keys will have their LEDs lit when those Features or options are active An error or tone will sound if a key that is unavailable is pressed or if a parameter that is out of range has been keyed in The system will not accept out of range parameters CAUTION J The Start Stop button LED is illuminated while the experiment cycle is active even if ultra sonics is not currently On i e
6. 1 3 2nd Review copyfilename C Documents and Settings St phanie My Documents CD ROM Sonifier Manuals Digital Englishindex fm modified Thursday August 23 Index BRANSON K keypad entering parameters 6 23 keypad description 2 3 L language Setup eire ete rte tarte ger ED ea Denuo 6 9 limitation of remedy and liability 1 6 limited warranty reper eee tent bert 1 4 limiting temperature rise A 1 M malHtenarce in n ee t eines 7 1 maximum temperature parameter Cnty eene anii 6 27 MESSAGES 6 41 micro reete a e D a 4 3 minimizing undesirable factors A 2 model SetUp ieiiee etie lax see 6 21 MOOS tS 6 1 O operating considerations A 1 Operation eere ei ae 6 1 operation parameters ucnnee 6 23 optional items enne 4 3 optional items parts 1 B 1 OVOLVIGW 11 1 a eai decedit tete ferte Got fuot 2 1 l 4 CONFIDENTIAL For Review Only 100 214 239 filename C Documents and Settings St phanie My Documents CD ROM Sonifier Manuals Digital English Index fm modified Thur
7. Fan does not operate when system is turned on Display screen appears Fan motor has failed Return for repair Fuse fails when system is turned on Fuse is under rated Mains Voltage is incorrect Fan motor has failed Power supply module has failed Unit failed due to connection to incorrect input voltage Verify voltage source is correct Damage may occur if connected to wrong voltage source Replace fuse with cor rect value and retry or return for repair Ultrasonic power is not delivered to the horn Ultrasonic power supply module has failed Digital controls have failed Return for repair Unusual noise from Horn when ultrasonics are on Horn or Tip is loose or it is contacting a solid object Reposition horn Remove examine and clean tip reinstall tip Horn or tip has failed Replace horn or tip 100 214 239 Rev A Digital Sonifier Table 1 Troubleshooting Charts System Trouble Analysis Chart Symptom Probable Cause Corrective Action Ultrasonic power is absent or inconsistent Foreign material is between horn surface and replaceable tip If horn is hot to the touch problem may exist with cor roded tip to horn interface Remove examine and clean tip reinstall tip Replace tip if corrosion is excessive If used with a Treatment Chamber horn tip is not immersed adequately Verify th
8. Print Print Area Off Newline Print Term Off None Send Send Area Send Term None Xfer Term Auto Answerback Keys Typewriter Do not make changes to the F9 F10 or F11 keys Doing so can affect the operation of the Wyse terminal with the Digital Sonifier Use the default settings 100 214 239 Rev A D 3 Wyse 55 Terminal Settings BRANSON For Your Notes D 4 100 214 239 Rev A Digital Sonifier Index Index A accessorie S ied m 4 3 accessories parts list C 1 aerosoling cnet tee ideis A 3 amplitude parameter i 6 29 assembling the equipment 4 6 B back panel 5 2 6 baud rate SQUUP E 6 17 biological materials effects of ultrasonic irradiation on A 4 block diagram iniecit lere EE ERREA 4 2 Branson howto cOhtaGlt cae ite n p eese 1 6 100 214 239 CONFIDENTIAL For Review Only 1 1 2nd Review copyfilename C Documents and Settings St phanie My Documents CD ROM Sonifier Manuals Digital Englishndex fm modified Thursday August 23 Index l 2 caution warning and error messages 6 41 compliance with RFI 1 3 component description 4
9. set the values and enable the Printer and Report modes The parameters available at System Setup and their possible values are described in the table below Detailed steps for each parameter are described in the pages that follow Table 6 1 System Setup Parameters Parameter Default User Selectable Options Printer Off Off On Remote Terminal Off Off On Language English English Spanish German Italian French Temperature F F C Time of Day 12 00 00 00 23 59 24 hour format only When the Language parameter is set to English the date format Date 01 01 1998 is MM DD YYYY For any other Language parameter the date for mat is DD MM YYYY Reports Disabled Disabled Enabled 9600 9600 1200 2400 19200 ee 9 Terminal Mone Model printer Panasonic Epson The system s Date clock 1s Year 2000 and Leap Year compatible and may be set up through the year 2069 100 214 239 Rev A 6 9 BRANSON Operation System Setup Language The Language parameter affects the displays and the printed reports 1 The Language choice also affects the Date format and the Reports sent to the Printer and or the Remote Terminal See page 6 12 for additional information on Setting the Date Take this action To get this result PRINTER OFF REMOTE OFF Press the Setup key to display the System Setup menu Its LED li
10. time and does not include the Pulse Off time 100 214 239 Rev A 6 27 Operation BRANSON Parameter Entry Maximum Temperature The Maximum Temperature parameter 1f used identifies the maximum temperature allowed in the experiment Maximum Temperature may be used as a Limit for all modes except Pulse Pause Mode CAUTION J Setting the MAX TEMP and TIME parameters together can cause the system to operate ina temperature limiting fashion which can cause ultrasonics to be suspended briefly and then reactivate to continue a process cycle without warning Do not handle the Horn or Converter if using MAX TEMP and TIME in the Temperature Mode and ensure the process cycle is Stopped before touching the Horn or injury could occur If used with the Pulse Pause mode or using Maximum Temperature and Time parameters together Maximum Temperature sets the upper temperature parameter of these modes at which point the experiment will pause to allow cooling but does not cause the experiment to Stop Take this action To get this result Use the Up and Down arrow keys if necessary to display the line showing the Max Temp parameter 0 100 PRESET 1 TIME hr m s MAX TEMP AMPLITUDE ___ Press the Line Select key that corresponds to Max Temp PRESET 10 __ TIME _ __ 1__ hr m s TEMP AMPLITUDE ___ 6 28 100 214
11. 9 Pack carefully in original packing material to avoid damage in shipment 10 Return general repairs by any desired method Send priority repairs by air freight 11 Prepay the transportation charges FOB Danbury Connecticut U S A Notes 1 6 100 214 239 Digital Sonifier How to Contact Branson 100 214 239 Returning Equipment for Repair to Geneva facility NOTE To return equipment to Branson you must first obtain an RGA number from a Branson representative or the shipment may be delayed or refused If you are returning equipment to Branson for repair you must first call the Danbury Repair department to obtain a Returned Goods Authorization RGA number If you request it the repair department will fax a Returned Goods Authorization form to fill out and return with your equipment Branson Ultrasonic SA 9 chemin Faubourg de Cruseilles P O Box 1031 CH 1227 Carouge Geneva Phone 41 22 304 83 58 Fax 41 22 304 83 59 Provide as much information as possible that will help identify the need for repair Carefully pack the equipment in original packing cartons Clearly label all shipping cartons with the RGA number on the outside of cartons as well as on your packing slip along with the reason for return Return general repairs by any convenient method Send priority repairs by air freight You must prepay the transportation charges FOB Danbury Connecticut U S A Obtaining Replacement Parts You can
12. The Tissue Disruptor may also be used alone The Tapered Microtip attaches directly to a standard 1 2 inch tapped Disruptor horn The amplitude at the end of a tapered tip is three and one half times greater than that of the stan dard horn The tapered tip is recommended for difficult applications such as spores fungi yeast muscle and connective tissue Excellent results can be achieved on volumes ranging from 3 to 20 ml in a comparatively short period of time The diameters of the tapered micro tips are 1 8 inch 3 2 mm 3 16 inch 4 8 mm and 1 4 inch 6 4 mm The stepped microtip is a two piece unit consisting of a Coupling Section and a lower double step tip Because the coupling section 1s attached directly to the converter the standard disrup tor horn must be removed prior to using the stepped microtip Recommended for use on extremely small volumes the stepped microtip can be used to treat volumes ranging from 0 5 to 20 ml Applications for this tip include ted and white blood cells tissue culture cells HeLa cells and the complete range of cells that have low to medium resistance to breakage To prevent foaming or aerosoling while processing small quantities with the tapered or stepped mictottps the use of a conical shaped tube such as a reaction vial or a cut down centrifuge tube is tecommended CAUTION J When using microtips do not exceed a maximum Amplitude Control setting of 70 The microtip will break if driven at hi
13. This will cause improper operation but will not damage the unit Pins 3 and 4 must be tied to pin 6 for operation of the Temperature Limit and Test Run ning LED s respectively as shown Power Amplitude and Temperature must be referenced to pin 14 for proper signal measurement 4 16 100 214 239 Rev A Digital Sonifier Guards and Safety Equipment 4 7 Guards and Safety Equipment Although the Digital Sonifier operates outside the normal range of human hearing some appli cations can create audible noise above 85dB If an uncomfortable level of noise is present the operator should wear ear protection for safe operation Appropriate eye protection should be worn when operating the Digital Sonifier to prevent possible splash injury originating in the solution WARNING The Ultrasonic Horn can cause injury and or equipment damage during oper ation To avoid injury or accident never touch the Ultrasonic Horn while the System is turned on and do not allow the Horn to come in contact with solid vessels or supports The User I O may be used to remotely control the System If this is the case you must design in whatever safety precautions are appropriate to your User I O circuit design to prevent unex pected start up which can cause personal injury and can cause equipment damage 100 214 239 Rev A 4 17 Installation and Setup 4 18 4 8 Ultrasonic Test BRANSON The Test button on the front panel of the Digital Sonif
14. To Save a set of parameters as a Preset follow this procedure Step Action to Save Presets Press the Save key on the keypad or type an SV command from the remote computer 1 or terminal The system displays the Save To entry screen Using the keypad enter a value for the preset ID number You can use values from 01 to 20 Press Enter to save the value The system displays a message asking you to confirm that you want to overwrite the existing preset even if you chose an unused Preset ID 3 Press the appropriate Select key for Yes to replace the previous preset with the one you are setting and Save your Setup or No to return to the previous screen and give the current preset a different ID number 1 The system will always confirm the overwrite procedure as a level of security for all presets even if the user had not previously used that Preset ID To Recall a saved Preset follow this procedure Step Action to Recall a Saved Preset 1 Press the Recall key or type an R command from the remote computer or terminal The system displays the Recall entry screen 2 Using the keypad or the number keys on your Remote Terminal or Computer enter in the desired preset number 3 Press Enter The system reloads the preset information to memory and is ready to run with those parameters The system returns to the previous page of the screen 6 40 100 214 239 Rev A Digital
15. and the amount of use Periodically inspecting the tip will help you recognize erosion early As erosion progresses the color of the tip changes from its original polished appearance first to light grey and then to dark grey Concentric rings begin to appear and finally the tip becomes rough and pitted resulting in loss of power output As it erodes the tip can also introduce metal particles into the solution causing it to darken or discolor Eventually erosion may become significant at which point the tip will require replacement 100 214 239 Rev A 7 1 Maintenance BRANSON 7 2 Power Output Loss There are several conditions that can cause a decrease in or loss of power output including operating with a faulty power supply or poor electrical connection operating with a loose horn converter connection and operating with a cracked or corroded horn tip assembly If your unit indicates a decrease in power output first check the Converter Cable connections then take the following steps to ensure that the horn tip assembly is not loose or cracked or corroded Fretting corrosion refers to a black crusty build up resulting from friction between metal parts that appears on the mating metal surfaces Corrosion can reduce or alter system performance Examine all mating surfaces microtip to converter disruptor to converter tip to horn and wipe the surfaces clean with a clean cloth or paper towel The 250 450 Sonifier does not
16. minutes Streptomyces monocellular elements from surface grown colonies obtained 1 minute Complete disruption 5 minutes 50 disruption in 2 minutes Subcellular particles may be separated or broken depending on power selection and length of time Sulfanilamide excellent dispersion in less than 1 minute Continued ultrasonic treatment will produce complete disruption Sulfapyridine excellent dispersion in less than 1 minute Continued ultrasonic treatment will produce complete disruption Synovial fluid ultrasonic treatment 15 an excellent means of reducing fluid viscosity It is sim pler and faster than using hyaluronidase T pyriformis excellent disruption 8 enzymes released Tablets complete disruption in 2 40 seconds depending on type Tea excellent extraction Tetrahymena disrupts in a few seconds Enzymes which have been monitored include succinate lactate B hydroxy butyrate glutamate and DPNH oxidases DPNH cytochromeC reductase and ribonuclease Activity of DPNH oxidase was twice that of the best previous experiments Themoactinomyces disruption of hyphae Homogenization of protein complex without denaturation Thermophile negative good disruption within 2 minutes 100 214 239 Rev A A 13 Application Information BRANSON Thermophilic bacillus isocitrate lyase was extracted from a spore forming bacillus similar to Stearothermophilus A washed cell paste suspended in a phosphate buffer was
17. 3 degrees below the MAX TEMP setting at which point the timer and the ultrasonic vibrations resume The front panel display shows the proper ultrasonics active displays Pulse Pause Mode Used for holding an experiment within a desired temperature range Ultrasonics are applied to the sample while keeping the sample s temperature between the Pulse Temperature value and the Maximum Temperature values that you specify This allows you to limit temperature buildup in the sample while continuing ultrasonic treatment until you obtain the process results that you want 6 2 100 214 239 Rev A Digital Sonifier Setup and Operation Pulse Pause Process Cycle In Pulse Pause Mode the system applies ultrasonics either Pulsed or not to the sample until it reaches the Pulse Temperature The system then adjusts or sets the Pulse On Pulse Off times for this continuing process in order to slow the heating process of the sample When the experiment reaches the Maximum Temperature ultrasonics are turned off temporarily until the sample s temperature drops three degrees either Celsius or Fahr enheit at which point ultrasonic treatment resumes using the Pulse On time Pulse Off time ratio calculated by the system until the Maximum Temperature 1s reached and ultrasonics are once again turned off This pro cess 1s repeated until you manually stop the experiment or until the Time that you specified as a limit has elapsed While the experiment is running the
18. DISP 80 Cursor Blink BIk BRANSON Scrn Saver Lines 25 Display Reverse Char Cell Page 1xLines Autopage 80 132 Clr F2 F2 Key Setup GENRL Personality 220 8 Enhance On Status Line Off Scrl Jump Autoscrl On Wrap EOL Off Rcv CR CR Monitor Off jud Off F3 F4 Key Setup KEYBD Keyclick On Repeat On Keycode ASCII Keylock Caps Language US Corner Key Funct F4 F4 Key Setup COMM Comm FDX Xmt Lim None Ewa Or Mode Mdm Rc Hsk None Monnet 192 5 Aux Rc Hsk None Send ACK On Aux Xmt Hsk None F5 F5 Key Setup PORTS Mdm Baud Mdm Data Mdm Stop Rate 3600 Parity S None Bits Aux 9600 DUX Data 8 None Aux Stop Bits 1 Rate Parity Printer Nulls Host Port Modem Port Attached On Suppress On D 2 100 214 239 Rev A Digital Sonifier F6 F6 Key Setup MISC WPRT Intensity Normal WPRT Rev Appendix D WPRT Undrin BIk End CRLF ETX Attribute Multiple Page Margin Bell Off Bell Volume F7 F7 Key Setup ANSI1 Char Set Multinational Char Mode Multinational Rest Act Times ANSI ID VT100 Cursor Keys Normal Keypad Numeric DEL DEL CAN Feature Lock Off Fkey Lock F8 F8 Key Setup ANSI2
19. OFF period the power goes to zero Keypad Disabled If the keypad is disabled the LCD display on the front panel shows one of the following items KEYPAD DISABLED message EXPERIMENT RUNNING message Power display bar graph During the experiment only the Start Stop and Pause keys are active When the experiment is finished the EXPERIMENT RUNNING message changes to EXPERIMENT COMPLETED Remote Terminal Displays Ifa remote computer or terminal is connected and active the system displays the EXPERIMENT RUNNING message on the screen every five seconds while the experiment is running See page 6 35 for more information about the Remote Terminal option and its commands 100 214 239 Rev A 6 43 Operation 6 9 Warning and Error Messages BRANSON When the system encounters an error condition a message 1s displayed on the front panel of the Digital Sonifier unit and on the remote computer or terminal 1f one is enabled If the keypad 1s in lockout mode messages will appear only on the remote computer or terminal The following table describes the possible error and warning messages the conditions that cause them and the actions you should take when they are displayed Table 6 5 Warning and Error Messages Message Cause Take This Action Value out of range You entered a parameter value that was outside the parameter s limits Press Enter The parameter becomes available so that you can re enter a cor
20. Select key that corresponds to Reports BAUD RATE 9600 REPORTS DISABLED DATA BITS 8 PARITY 0 100 6 22 100 214 239 Rev A Digital Sonifier Entering System Setup Parameters BAUD RATE 9600 Press the same Line Select key again REPORISEDISODEED to toggle between the possible values for DATA BITS 8 Reports Enabled or Disabled PARITY NONE BAUD RATE 9600 Press the Enter key to store the value REPORTS DISABLED for Reports DATA BITS 8 PARITY 0 100 Press the Setup key to exit the System Setup menu The Setup LED extinguishes 100 214 239 Rev A 6 23 Operation BRANSON 6 4 Entering Operation Parameters You can enter Operation parameters through the keypad or through the remote terminal if the terminal is enabled in the System Setup menu Only the Amplitude value using the Amplitude knob on the side of the chassis may be changed when an experiment 1s running When the System 1s in the Ready mode e g after power up or after a test or experiment is performed it displays the first page of the Parameter Entry menu In the Parameter Entry menu you can enter parameters for your test or experiment When a Line Select key for a parameter is available and may be selected its LED 1s lit Entering Parameters from the Keypad When in the Ready mode you can press any available Line Select key on the front panel to select a parameter for modification Ther
21. Sonifier 6 7 Printing Sending Reports Printing Sending Reports After an experiment the system can produce a report showing the date and time of the experiment the param eters in effect and statistics for energy power amplitude and temperature The report data remains available until you start a new experiment or until you change any System Setup parameters Reports must be turned on in the Setup menu If you want to run a report you must do so before the next experiment is started and before you change any System Setup parameters You can send the report to a printer or to the remote computer or terminal The report has the following format TIME XX XX DATE XX XX XXXX PRESET ID value TIME value Z D x lt value ON value PULSE OFF value P ULSE TEMP value ENERGY energy delivered in Joules PEAK POWER power delivered in watts AMPLITUDE last amplitude LAST EMPERATURE READING temperature HIGHEST TEMPERATURE temperature TIM FJ 100 214 239 Rev A TIME elapsed time elapsed time 6 41 Operation BRANSON 6 8 Displays During the Experiment Runtime Displays While the experiment is 1n progress the system shows certain runtime data on the LCD display and on the remote computer or terminal if one is enabled The data is refreshed on the
22. and 200 245 Volt export models available factory set Multiple languages for display messages English Spanish Italian German or French Front panel LCD display showing parameter settings alarms and messages Membrane front panel with numeric keypad for parameter entry and function selection Digital parameter setting with valid parameter range checking Twenty 20 presets for different parameter Setups Digital Wattmeter Front panel Bargraph display of relative power while running Amplitude control analog adjustable while running Pulse or Continuous operation Temperature option Sample Hold using Pulse Pause operation Digital Timer 9 hours 59 minutes 59 seconds Automatic end of cycle using Limits Factory tuned 20 kHz ultrasonic design requires no user adjustment Parallel Printer interface Printed Report capability with date stamp and parameter information Serial interface for remote computer or terminal User I O offers remote start stop control For additional feature information please contact your Branson representative 100 214 239 Rev A 2 9 Introduction to the Digital Sonifier For Your Notes 2 10 BRANSON 100 214 239 Rev A Digital Sonifier Delivery and Handling 3 Delivery and Handling 3 1 Delivery and Handling The Digital Sonifier has no special handling constraints On receipt of your Digital Sonifier sys tem take the following steps Inspect the carton for signs of damage Ope
23. display every second The Start Stop LED on the front panel also indicates that an experiment is running The following front panel displays will be seen when the system is running an experiment RUN TIME 0 43 27 PROBE TEMP z 67 6 F The first page of the runtime display Note the bargraph display on the bottom line PULSE ON If you wish you see the second page press the Down Arrow button MAX TEMP 104 7 F The second page of the runtime display PULSE TEMP F If you wish to see the first page press the hr m s pan ON Outen POWER 146 Watts Start Stop key and LED The Start Stop key is used to manually Stop an experiment at any time even during Remote mode with the keypad locked While the experiment cycle is running or active the LED next to the Start Stop button is lit Normally the Start Stop key is used to Start or Stop an experiment from the front panel but the front panel controls may be locked if using the Keypad Lock parameter from the remote computer or terminal Pressing the Start Stop key or OFF command while an experiment is paused will end or stop the paused experiment Pause key and LED The Pause key is used to manually Pause an experiment at any time even during Remote mode with the keypad locked When you pause operation by pressing the Pause key on the keypad or by typing an H command through the remote computer or terminal the display shows a message stating that op
24. during an OFF period in a Pulsed mode Be careful to not touch the Horn when a cycle is active as it may start without warning and could cause injury Each menu line in the display shows a parameter whose value you can change if tts associated Select key LED is lit The parameter can have either a numeric value that you enter with the numeric keys or a choice that you select by toggling between a set of possible selections You select a parameter for entry by pressing the Line Select key next to it Then you key in the desired numeric value using the numeric keys or the appropriate choice from a sub menu using the Line Select keys When you press a Line Select key for a numeric parameter the least significant digit of the parameter value flashes and the numeric keys become active You can Key in anew value for the parameter replacing the old value Correct the value you are entering by pressing the Clear key to erase the value You also use Clear to delete a parameter value to turn a parameter OFF Press a different Line Select key to select a different parameter for entry cancelling any changes you have made to the first parameter Press an active Page Up or Page Down arrow key to view a different page of the Parameter Entry menu Press the Enter key to store the new value or to store a blank or an OFF value if you pressed Clear When you begin entering a value the old value 1s replaced the display by the new on
25. glass surface areas plus circulation through the arms pro vide an effective means of heat exchange Model 25 8 25 ml 201 123 001 Model 50 25 180 ml 201 123 002 Model 250 35 300 ml 201 123 003 Tissue Disruptor Designed for disintegration of difficult tissues Stainless steel construction Cell bottom holds 6g of tissue A water jacket is provided for cooling 101 021 007 Soundproof Enclosure Reduces mechanical noise generated during liquid processing to a normal level Especially useful when using a cell disruptor for extended periods 101 063 275 Glass Beads For cell homogenization Waterproof 1 pound packages 1 2 mm diameter 201 002 004 25 micron diameter 201 002 003 35 micron diameter 201 002 005 100 214 239 Rev A C 3 Accessories Parts List BRANSON For Your Notes C 4 100 214 239 Rev A Digital Sonifier Appendix D APPENDIX D Wyse 55 Settings Wyse 55 Terminal Setup Parameters The following setup 1s for the Wyse model WY 55 Terminal with the extended PC style key board available as a kit from Branson using EDP 200 103 228 All Wyse terminal Set Up is done directly from the Wyse terminal s PC style keyboard once it is set up and plugged in Refer to the Wyse terminal box for manufacturer s instructions Programming the Wyse 55 Terminal Before the Wyse 55 can be used with the Digital Sonifier the communications
26. is 4 Press the Test button on the front E a sound The n a ay anel Observe the front panel display WE SOOW SOIS P test will run for 2 seconds then stop If the system showed readings on the display during the Test Verification that the Digital Sonifier is 5 you may either proceed with your operating and is ready to be set up experiments or Turn the unit off for your experiment 100 214 239 Rev A Digital Sonifier Technical Specifications 5 Technical Specifications 5 1 Technical Specifications 5 1 5 2 Physical Description 5 2 5 3 System Performance Benchmark 5 3 5 1 Technical Specifications The following table outlines the technical specifications for the Digital Sonifier Digital keypad entry for parameters 4 line x 20 character LCD display rotary Amplitude output control remote terminal con trol printer output main power switch temperature probe input Controls and Displays Adjusts amplitude of power supply output voltage Pmplitugo enirel Range 1096 10096 of nominal converter amplitude Intermittent pulse duration adjustable 0 1 seconds 59 9 seconds or continuous processing time Horn Frequency 19 850 20 050 kHz 115V 10 50 60 Hz North American unit nine Voltage 200 245 10 50 60 Hz Export only Po
27. key to store the value PULSE OFF SEC for Pulse On or press the Clear key to re enter the value PULSETEMP PROBE TEMP D EB G 9 As soon as you have pressed Enter PULSE ON SEC to store the Pulse On value the system moves to the Pulse Off parameter line PURSE OEE uL SEC PULSETEMP SEA OMe 806 Use the numeric keys to enter the Pulse Off time in tenths of seconds PROBE TEMP C PULSEON SEC Press the Enter key to store the value PULSE OFF SEC for Pulse Off or press the Clear key to re enter the value o A PULSETEMP PROBE TEMP Bc lear EB NOTE 1 A shortcut tip If you Clear either Pulse time parameter and press Enter both values will be cleared You do not need to Clear both parameters 100 214 239 Rev A 6 31 Operation BRANSON Parameter Entry Pulse Temperature The Pulse Temperature is the lower temperature parameter of the Pulse Pause mode temperature range Entering a value for the Pulse Temperature enables the Pulse Pause mode clearing this value disables this mode CAUTION J Setting the PULSE TEMP parameter to a numeric value will activate the Pulse Pause Mode process This can cause the system to operate in an On and Off fashion which can cause the ultrasonics to be suspended for a period of time and then reactivate to continue a process cycle without warning Do not handle the Horn or Converter if the Puls
28. liquid surface level Foaming can be detected by a change the sound level and a fluctuating reading on the power bar graph When aerosoling occurs little or no energy couples reliably to the solution and excessive top layer heating results Remedy this problem by placing the probe as deep as possible and setting the Amplitude control to 10 or 20 for a few seconds Then gradually increase the Ampli tude control to the level required Discoloration of the Processed Sample If the tip touches the side of a glass tube or beaker small glass particles are released which gradually changes the sample to a greyish color Excessive tip corrosion can also cause a grey ing or darkening condition Sterilizing and Preventing Cross Contamination You can sterilize horns and tips by removing them from the converter and autoclaving them It is faster easier and equally effective however to sterilize horns by immersing them a bea ker of alcohol or other disinfectant and then turning the power on for a few seconds This tech nique also removes unwanted residue from the horn and tip 100 214 239 Rev A A 3 Application Information BRANSON A 4 Disrupting Tissues and Solids You can effectively homogenize or disrupt many kinds of tissue and other solids Energy radi ates only from the horn s tip The energy is most concentrated within 1 2 inch 12 7 mm of the face of the tip When you treat tissue solids in solution the free
29. of Digital Sonifieron page 2 8 for the RS 232 connector location The RS 232 connector pin out is provided below the connection only supports three data leads Ground RxD and TxD and does not support handshaking see Remote Computer or Terminal on page 6 34 for other Setup information The serial connection requires a straight through cable with a 9 pin DB9 male connector with the following leads supported the remote end of the cable should match your Remote Terminal device RS 232 Connector Pin out pr rbi Pin Designation Common Signal Name 2 RxD Received Data at unit 3 TxD Transmitted Data from unit 5 Gnd Signal Ground The other leads of the RS 232 connector DB9F are not supported The remote terminal connection should not exceed a 50 foot 15 meters cable length using conventional data cabling 4 6 3 Terminal Connection A Terminal is a display option that is available through Branson The terminal is used as a remote terminal device and connects to the unit as such In order for the terminal to operate with the RS 232c serial port of the Digital Sonifier its configuration options must be set to match those of the Digital Sonifier s output 4 6 4 Printer Connection The printer is connected to the back panel of the Digital Sonifier female DB 25 connector to a conventional 25 pin parallel printer cable Your printer cable must have the male DB 25 connector Plug the printer cable into the conn
30. of the Digital Sonifier 15 equipped with An IEC type power cord connection for connecting the power supply to a grounded electrical outlet A fuse holder for access to a replaceable 5x20mm protective fuse The fuse is a glass slow blow type refer to the data tag for the fuse rating A converter cable with connector to connect the power supply to the Converter An RS 232 connector female DB9 for connecting a remote computer terminal to the unit This connection requires a cable with a 9 pin male connector Serial ASCII data is transferred through this connection at a user selectable baud rate of 1200 2400 9600 or 19200 bps with 8 data bits 1 stop bit and no parity RX TX and Signal Ground are supported A parallel printer connector female DB25 requiring a 25 pin male connector During System Setup you can select the dot matrix printer choices from Pana sonic Epson or IBM A User I O connector female DB15 requires a cable with a 15 pin male DB15 connector The User I O provides proprietary system control signals A 1 4 inch phone jack style connector for an optional temperature probe 100 214 239 Rev A Digital Sonifier System Features 2 3 System Features The Digital Sonifier includes the following features Front panel system On Off switch Separate front panel Stop and Pause buttons for experiment cycles Power output of up to 200 watts Model 250 and up to 400 watts Model 450 Nominal 117 Volt North American
31. protocol infor mation of the Wyse 55 terminal must be set to match it Information on how to program the Wyse terminal is included 1n the instruction sheet packed with each terminal The following parameters are displayed on the Wyse terminal during its setup The options listed in the following tables are important for correct operation A very few ate critical and they are highlighted below Other parameters within the Wyse terminal should not be changed from the values listed The printing capability of the Wyse terminal is not supported at this time Wyse 55 Terminal Parameters Set the function key parameters on the Wyse 55 terminal for correct operation with the Digital Sonifier Parameters that are highlighted 1n the following lists are important to its functioning with the Digital Sonifier Step Operation 1 Connect the Wyse 55 terminal and turn it on 2 Press the SHIFT and SELECT keys together The terminal is placed in its Setup mode 3 Press a function key F1 thru F8 to change the Wyse set up When done press the F12 key to access the Save screen 4 Press the Spacebar to toggle the Save options All Yes No until you have Yes showing if you wish to save your changes or No if you wish to ignore your recent changes 5 Press F12 again to exit the Wyse Setup mode 100 214 239 Rev A D 1 Wyse 55 Terminal Settings Wyse 55 Function Key Setup Parameters F1 Columns F1 Key Setup
32. terminal IPOF PULSE OFF Type the time period when power will be off and press Enter on your remote computer or terminal IPPT PULSE TEMP Type the Pulse Temperature and press Enter on your remote computer or terminal Type the identifying number under which you want to ISV PRESET ID save the preset parameter values and press Enter on your remote computer or terminal Type the preset ID number for the saved set of param IR PRESET ID eters you want to use and press Enter on your remote computer or terminal IRP List of parameters The system displays all the current parameters on the remote computer or terminal IS Buon The system sends a report to the remote computer or terminal IP N A The system sends a report to the printer The experiment is currently paused IH EXPERIMENT PAUSED Issuing another pause command from the remote terminal will cause the experiment to resume if the experiment is currently paused TUNING TEST ITS PLEASE WAIT 2 SEC Ultrasonics are activated for a two second test IKL KEYPAD DISABLED The keypad is disabled or enabled if it was already dis abled EXPERIMENT RUNNING ION The experiment is running from a remote command displayed every 5 seconds IOFF EXPERIMENT STOPPED The experiment has been stopped 100 214 239 Rev A 6 35 BRANSON Operation System Setup Remote Computer Terminal The Remote parameter enables or disables support for a cu
33. the converter and tighten using spanner wrenches The recommended torque is 220 inch pounds 24 85 Nm A standard flat tip recommended for processing liquids 1s supplied with tapped horns Other tip configurations are available for experimental work on applications where the ultrasonic vibrations are transmitted directly into the solution The shape of the horn influences the direc tion which the ultrasonic vibrations are delivered from the horn Connecting the Tip to the Horn attach the tip to the horn take the following steps Step Action Clean contacting surfaces of the horn and tip and remove any foreign matter from the threaded stud and threaded hole CAUTION The tip must be installed clean and dry or the power supply may not tune and operate correctly Hand assemble the tip to the horn Using a spanner wrench on the horn and an open end wrench on the tip tighten the tip See Figure 4 3 Torque specifications for the various threaded tips follow 1 4 20 tighten at 90 inch Ibs 10 16 Newton meters 3 8 24 tighten at 180 inch lbs 20 33 Newton meters 100 214 239 Rev A Digital Sonifier Assembling the Equipment Tightening a tip on a horn using spanner wrench and open end wrench Converter Spanner Horn Tip 4 Tip Wrench 100 214 239 Rev A 4 11 Installation and Setup BRANSON 4 4 3 Horn Amplitudes Use the following charts to determine the co
34. their respective owners EDP 100 214 239 Rev A February 2001 Digital Sonifier User s Manual Forward Congratulations on your choice of a Branson Ultrasonics Corporation system The Branson Digital Sonifier system is the newest generation of product using this sophisti cated technology for a variety of customer applications This manual should be kept with the system Thank you for choosing a Branson product Introduction to this manual This manual is arranged into seven structured chapters which will help you find the informa tion you may need to know to safely handle install set up program operate and or maintain this product Additional information 1s provided in several Appendix chapters In the event you require additional assistance or information please contact our Product Sup port department see How to Contact Branson on page 1 5 for information on how to contact us ot your local Branson representative BRANSON For Your Notes 100 214 239 Rev A Digital Sonifier 100 214 239 Rev A Digital Sonifier Safety Considerations 100 214 239 1 Safety and Support 1 1 Safety Considerations 1 1 12 General Precautions 1 2 1 3 Regulatory Compliance 1 3 1 4 Warranty 1 4 1 5 How to Contact Branson 1 5 1 1 Safety Considerations CAUTION The following safety considera
35. to the Digital Sonifier BRANSON Operation of the Digital Sonifier can also be controlled through a remote computer terminal or User I O device You always have the ability to halt operation whether control is direct or remote Each key on the front panel of the unit except for the numeric keys Print and Test has an LED indicator near it When the key 1s active the LED 1s turned on Some of the keys on the Digital Sonifier keypad have equivalent commands preceded by that can be entered through the remote terminal s keyboard Section 2 2 1 Digital Sonifier Keypad Description beginning on page 2 4 describes each key on the Digital Sonifier keypad and identifies its equivalent remote terminal command preceded by an excla mation point if applicable Section 2 2 2 Remote Terminal Commands beginning on page 2 7 provides a detailed description of all remote terminal commands Section 2 2 3 Back Panel Connections on page 2 8 describes the back panel of the unit 2 2 1 Digital Sonifier Keypad Description LCD Display The front panel is equipped with a four line 20 character display screen where menus parameter options mes sages and warnings ate displayed If the data being displayed has more than one page you can navigate up and down in the display by pressing the Page Up and Page Down arrow keys PRESET ID 16 TIME 0 12 52 hr m s If you wish to see the second page press MAX TEMP 104 3 F he D A the Down
36. 01 021 005 25 50 101 021 006 Flat Tip Replacement for 1 2 horn 1 4 20 thread 101 148 013 Tapered Microtips For processing small volumes Attaches to standard tapped disruptor horn Tip amplitude is 3 1 2 times greater than that of standard horn Recommended for difficult applications such as spores fungi yeast muscle and connective tissue Excellent results on volumes ranging from 3 to 20ml in a comparatively short time 1 8 diameter 101 148 062 3 16 diameter 101 148 069 1 4 diameter 101 148 070 100 214 239 Rev A Digital Sonifier Appendix C Item Description Branson EDP Number Double step Micro Tip Assembly A two piece horn consisting of a coupling sec tion and a lower tip The standard disruptor horn must be removed prior to using this tip Recommended for use on extremely small volumes 0 5 20 ml Applications include red and white blood cells tissue culture cells Hela cells Overall length is 9 1 8 with 1 8 diame ter in the lower 2 1 8 NOTE The double step microtip is to be used only with coupler 109 067 683 Double step Micro Tips Coupler section only 101 147 050 Micro Tip section only 101 148 063 Rosett Cooling Cells Borosilicate glass cell has conical shape with three arms to allow circulation of substance being processed When the cell is immersed in a cooling bath the enlarged
37. 2 components uy 4 2 connecting the tip to the 4 8 connecting tips horns and converters 4 7 connection itio t 4 11 remote terminal sss 4 10 connections back panel eee eae 2 6 continuous flow 4 5 eic 2 2 front panel iei 6 3 converters connecting n E RE RR REEL des 4 7 cross contamination CUP i 4 4 date SGtup iie 6 15 description pliysiGal iste piede nens 5 3 Digital Sonifier introdBlctlon scie is ct ILIA 2 1 keypad description 2 3 OVENI EW deat thereat 2 1 technical 5 1 discoloration oni A 3 displays during the experiment 6 40 disrupting tissues and solids A 3 CONFIDENTIAL For Review Only BRANSON 100 214 239 filename C Documents and Settings St phanie My Documents CD ROM Sonifier Manuals Digital Englishundex fm modif
38. 239 Rev A Digital Sonifier Entering Operation Parameters PRESET IDz TIME _ __ __ hr m s Use the numeric keys to enter the value for Max Temp MAX TEMP AMPLITUDE ___ See moe melet 0 100 PRESET 10 __ Press the Enter key to store the value TIME _ __ ___ hr m is for Max Temp or press the Clear key to MAX TEMP or correct and re enter the temperature AMPLITUDE ___ lear E 0 100 100 214 239 Rev A 6 29 Operation BRANSON Parameter Entry Pulse On and Pulse Off The Pulse On and Pulse Off parameters identify the duration of the repeating Ultrasonics On Pulse and of the period in between On Pulses respectively used in the Pulse mode and Pulse Pause mode Take this action To get this result PULSE ON SEC Use the Up and Down arrow keys if PULSE SEC PULSE TEMP PROBE TEMP us necessary to display the line showing the Pulse On parameter PULSEON SEC Press the Line Select key that corresponds PULSE nr to Pulse On PULSE C PROBE TEMP E PULSE ON __ _SEC Use the numeric keys to enter the PULSE OFF __ _ SEC Pulse On time in tenths of seconds that the ultrasonic pulse will be on PULSETEMP __ PROBE TEMP iu S te ir Bote OMS 6 30 100 214 239 Rev A Digital Sonifier Entering Operation Parameters PULSEON SEC Press the Enter
39. 39 CONFIDENTIAL For Review Only 1 7 2nd Review copyfilename C Documents and Settings St phanie My Documents CD ROM Sonifier Manuals Digital Englishindex fm modified Thursday August 23 Index 1 8 technical specifications for the Digital Sonifier 5 1 temperature 10 o etd LETT 6 11 temperature 4 11 temperature rise and vessel A 2 EE A 1 terminal eee D ea etuer 6 37 terms and conditions of 1 3 4 11 time parameter 6 25 time of day i o 6 13 Up Eroso Pee 7 1 tips CONNECHNG enan ROGER RR 4 7 tissue disruptor opsioni a ea 4 5 tissues disr pting A 3 troubleshooting siiin ee 7 1 ultrasonic irradiation effects on biological materials A 4 ultrasonic 4 11 undesirable factors minimizilg cesset e Sena ah eee ats A 2 user VO 4 10 using glass powders with solution A 4 vessel capacity and speed of temperature rise A 2 CONFIDENTIAL For Review Only BRANSON 100 214 239 filename C Documents and Settings St phanie My Documents CD ROM Sonifier Manuals Digita
40. 4 4 Assembling the Equipment beginning on page 4 9 4 Use a spanner wrench on the horn and an open end wrench on the tip to install the tip using the following torque specifications 1 4 20 tighten at 90 inch lbs 10 16 Newton meters 3 8 24 tighten at 180 inch lbs 20 33 Newton meters 7 4 100 214 239 Rev A Digital Sonifier Troubleshooting Charts 7 2 3 Stud Reinsertion The Stud is intended to be use only once part as it has knurls on its end which bite into the relatively softer horn material l he studs are also specially designed to withstand ultrasonic stresses If you must temporarily re use a used stud especially with an Aluminum horn it is very important to clean the threads and the horn of the previous shavings 1 Using a file card or wire brush clean any chips from the knurled end of the stud 2 Using a clean cloth or towel clean the threaded hole 3 Examine the knurled end of the stud If worn replace the stud Examine the stud and threaded hole for stripped threads Do not use a damaged ultrasonic horn or converter 1 Threaded studs cannot be re used in titanium horns 4 Clean the stud and the threaded hole before reinserting it 5 Apply one drop of Loctite to the stud and insert it into the horn 6 Retighten the stud Use the following torque specifications Stud Size Torque Specification Stud EDP number 3 8 24 x 11 4 in 290 Ibs 33 Nm 100 098 121 3 8 24 x 11 2
41. Arrow button AMPLITUDE 43 The first page of a typical Ready display PULSE ON 2 1 SEC PULSE OFF 1 6 SEC If you wish to see the first page press the PULSE TEMP The second page of a Ready display Up Arrow button PROBE TEMP 67 6 C 2 4 100 214 239 Rev A Digital Sonifier Controls and Commands Line Select Keys There are four Line Select keys to the right ofthe display When a Line Select key is active the LED next to it is turned on You can press a Line Select key to Selecta line in the Parameter Entry menu display for a parameter entry Toggle values in a parameter or System Setup submenus for example Yes or No Select an item from the options available when the system issues a warning error message Once a Line Select key 1s pressed its LED 1s turned off When you begin entering a value for a parameter you selected with a Line Select key all the other Line Select keys become inactive and their LEDs are also turned off until you complete the parameter entry If you have selected a line but have not begun to enter a value the other Line Select keys remain active and you can select another line on which to enter a parameter value Page Up and Page Down Keys The Page Up and Page Down arrow keys are active when you are displaying a menu that has more than one page You can use the Page Up and Page Down keys to move up and down in the menu If there ate more menu items above the page you are viewin
42. Digital Sonifier Models 250 amp 450 User s Manual EDP 100 214 239 Rev A BRANSON Ultrasonics Corporation 41 Eagle Road Danbury Connecticut 06813 1961 U S A 203 796 0400 BRANSON Manual Change Information At Branson we strive to maintain our position as the leader in ultrasonics plastics joining cleaning and related technologies by continually improving the designs circuits and compo nents in our equipment These improvements are incorporated as soon as they are developed and thoroughly tested Information concerning any improvements will be added to the appropriate technical docu mentation at its next revision and printing Therefore when requesting service assistance for specific units note the Release information found on the cover of this document and refer to the printing date which appears in the lower right corner of this page Patents and Copyright Copyright 1998 by Branson Ultrasonics Corporation Danbury Connecticut U S A All rights reserved Manual part number EDP 100 214 239 Rev A printed February 2001 Branson Ultrasonics Corporation equipment is manufactured under one or mote of the fol lowing U S Patents 4 249 986 4 315 181 4 363 992 4 551 690 4 651 043 4 786 356 4 973 876 5 095 188 5 435 863 Note Sonifier is a registered trademark of Branson Ultrasonics Corporation Danbury Connecticut U S A Other trademarks and service marks respectfully mentioned herein are held by
43. OVDC 10 Temperature Limit when the Temperature Limit is reached 11 Test or Running MuR ERE pope 12 Power Signal Output 0 10VDC 0 100 Power Out 13 Temperature Signal Output Go 14 Signal Ground Reference Signal Ground 15 not used no connection 100 214 239 Rev A 4 15 Installation and Setup BRANSON Each uset control application may be different it 1s left to the reader to design and create the customer end portion of a User I O interface Use the signal information provided on the pre vious page An example of one possible application 1s shown 1n the following diagram Example of Customer Provided Application using the User I O User I O DB15F on Digital Sonifier User Provided Interface to User I O 5VDC ea 9 pee Switch 2L _ LED 10 2 Temperature Limit s 2 rp 99 11 Test Running uo 4 500 12 e Power Signal 0 10V 5 Amplitude Signal 0 10V 13 Temperature Signal 0 10V 5VDC GND 6 14 e Ground Reference 7Z nc for Signals 151 Start switch can be tied to pins 1 and 9 as shown this will provide 5 volts in case the customer does not have a supply to operate user I O signals Pins 2 and 6 must be tied together for proper Start signal operation Note These must NOT be tied to earth ground Noise due to ground loops could be injected onto the signals
44. Parity parameters are non adjustable Take this action To get this result Press the Setup key to display the System Setup menu Its LED lights PRINTER OFF REMOTE OFF LANGUAGE ENGLISH TEMPERATURE F 0 100 Use Up and Down arrow keys if neces sary to display the line showing the Baud Rate parameter BAUD RATE 9600 REPORTS DISABLED DATA BITS 8 PARITY Press the Line Select key that corresponds to Baud Rate BAUD RATE 9600 REPORTS DISABLED DATA BITS 8 PARITY 6 38 100 214 239 Rev A Digital Sonifier Remote Computer or Terminal Press the same Line Select key again to toggle among the possible values for Baud Rate 1200 2400 9600 or 19200 BAUD RATE 9600 REPORTS DISABLED DATA BITS 8 PARITY Press the Enter key to store the value for Baud Rate BAUD RATE 9600 REPORTS DISABLED DATA BITS 8 PARITY 100 Press the Setup key to exit the System Setup menu The Setup LED extinguishes 100 214 239 Rev A 6 39 Operation BRANSON 6 6 Save and Recall Presets The Save and Recall functions allow you to enter a set of Operation parameters and save them under a unique ID number To repeat an experiment or test using the same set of parameters without having to re enter them with the exception of the Amplitude setting you can use the ID number to recall the saved parameters
45. The following steps are used only to record system performance not your experiment results NOTE 1 Make copies of the following page and keep it on file for future reference Use the following steps to create and record a benchmark for yout exact setup Step Action to Save a Benchmark 1 Make a copy of the Digital Sonifier Setup Form provided on page 5 4 Identify your experiment type on the form your you own reference 2 Set up the Digital Sonifier be ready to run and prepare your experiment Turn the Digital Sonifier system On Set your desired Amplitude control setting the value is displayed on the bot tom line of the LCD display and write the fill in the value on the form With the Horn submersed in your solution press the Start button located on 3 the bottom right hand side of the Sonifier s keypad Note any special immersion comments depth how it is supported etc Note the Bargraph reading the value is displayed on the bottom line of the LCD display on the form Press the Down Arrow key on the keypad and view the POWER reading the 5 value is displayed on the bottom line of the LCD display Record the POWER reading on the form Note any special adjustments settings operating modes or other system 6 variables that will be helpful at a later time in re creating your setup If you use a Preset note the Preset ID Turn the system off Record the serial numbers and horn information of the
46. a cooling bath of approx imately 35 C to maintain the sample at or below 5 C In comparison the processing of 200 ml would require a cooling bath of only 0 C to maintain the sample temperature Table 2 shows typical temperature rises for sample sizes of 25 ml and 100 ml using a 250 Dig ital Sonifier A polyethylene container was used with a 1 2 inch 12 7 mm diameter probe with a probe depth of 1 2 inch 12 7 mm anda starting temperature of 25 C Average Difference was the average difference among duplicate runs Table 1 Temperature Rise Variations Sample Size 25 ml 100 ml ger E 30 70 100 30 70 100 Seconds 30 60 Average Difference 0 7 0 4 0 4 0 0 2 1 6 100 214 239 Rev A Digital Sonifier Appendix A Minimizing Undesirable Factors Some factots may be detrimental to enzyme or biological activity and can reduce the effective ness of ultrasonic processing Minimize undesirable factors as follows Foaming or Aerosoling Always place the horn deep enough below the surface of the liquid to prevent violent motion agitation on the surface This problem is more critical when processing small volumes for example 0 3 to 5 ml A conical shaped tube or vial such as a cut down Eppendorf tube is recommended The shape of this type of container raises the liquid level without increasing the volume thereby permitting the horn to be inserted more deeply below the
47. a data entry field for the parameter 2 Enter the new value on the terminal following the format described on page 6 26 Press Enter on your remote computer or terminal The system checks the value for the proper format and range for the parameter If the value you entered is valid the system 3 accepts it displays OK on the remote computer or terminal and updates the LCD dis play on the unit s control panel if the active page contains that parameter If the value is invalid the system displays an error message Clear a value using the remote computer or terminal enter the parameter command as described in Step 3 above except when prompted to enter a value do NOT enter 4 any value but instead simply press the Enter or Return key on your terminal the exist ing value will then be cleared the system displays OK on the remote computer or ter minal and updates the LCD display to show that parameter as OFF or no value 6 34 100 214 239 Rev A Digital Sonifier Remote Computer or Terminal Table 6 3 Remote Terminal Commands Command Terminal Displays User System Action Type a value for the time and press Enter on your IT TIME remote computer or terminal IMT MAX TEMP Type the maximum temperature and press Enter on your remote computer or terminal IPO PULSE ON Type the time period when power will be on and press Enter on your remote computer or
48. art to recondition place the interface surface on the emery cloth Grasp the part at the lower end with your thumb over the spanner wrench hole and lap the part in a straight line across the emery cloth NOTE 1 Do not apply downward pressure The weight of the part alone provides suf ficient pressure Rotate the part 120 degrees 1 3 to the next hole Stroke the part an equal number of times at each rotation 2 or 3 Pick up the part and lap it once or twice in the same direction Rotate the part 120 degrees placing your thumb over the spanner wrench hole and lap the part the same number of times as described above 10 Rotate the part another 120 degrees to the next spanner wrench hole and repeat the lapping procedure OND Re examine the mating surface If necessary repeat steps 5 through 10 until you remove most of the contaminant This should not require more than two to three complete rotations for an aluminum horn or booster a titanium component may require more rotations 7 2 2 Horn Tip Cleaning Take the following steps to clean the horn s tip threads 1 If the horn has a replaceable tip remove it and clean its threads in alcohol 2 Swab out the end of the horn threads with a cotton swab and alcohol 3 Make sure both the horn and the tip are clean and completely dry before you reas semble them Refer to the Tip installation procedures for information on tightening the Tips This 15 found in Section
49. ates in a longitudinal direction and transmits this motion to the horn tip immersed in the solution which causes cavitation The implosion of microscopic cavities 1n the solution results causing the molecules in the medium to become intensely agitated The ultrasonic vibrations transmit ted through the horn can also be applied directly to a solid workpiece such as tissue through a vatiety of different tips that can be attached to the horn The Digital Sonifier is a constant amplitude device As the load or pressure the horn face increases the power supply develops more power to maintain the amplitude for any given out put control setting When the horn 1s operated in air it 15 subjected to minimum pressure and minimum power is required to maintain amplitude The load increases when the horn is immersed in a liquid the more viscous the liquid the higher the load and the more power developed If a flow through cell that can be pressurized is used thereby increasing pressure on the horn even more power 1s developed For any given application mote power results when a horn of higher amplitude or larger radiating surface 1s used when any horn 15 driven at higher amplitude by increasing the amplitude By setting various operation parameters you can precisely control the way in which ultrasonics ate applied to the sample You can Specify the time duration of the experiment Adjust the amplitude setting between 1096 and 10096 o
50. ced to less than micron each case slightly different Oil in water water in oil phases can be obtained tn same vessel Oyster shell small clean hole can be drilled with micro tip in 3 minutes No cracking is produced Paracolon excellent disruption Parasites separated from red blood cells in a few seconds Pasteurella pestis complete disruption 1n 30 minutes using high power Penicillium complete disruption 1n 5 minutes Pesticides ultrasonic treatment resulted in a 16 fold improvement in the potency of the antigen used with Microcrystalline Cellulose as a thin layer absorbent for chromatographic separation 100 214 239 Rev A Digital Sonifier Appendix A Phosphatidate phosphohydrolase the most potent inhibitors for this enzyme were obtained by making five dispersions with the Sonifier Cell Disruptor Phospholipid micelles produced stable preparations for an indefinite period Plant cells 30 packed plant cells W V and distilled water depending on type can be com pletely disrupted 1 15 minutes Plant tissue 1 gm dried tissue suspended alcohol disintegrates in about 5 minutes Platelets complete disruption according to size from 20 seconds to 4 minutes Pneumococci preserved in formalin for several years completely disrupts in 6 minutes Polio virus excellent disruption of this most difficult virus Powders broken down to a small relatively uniform particle size PPLO complete disruption in 2 minu
51. correctly customer provided User I O components have failed or are no longer functioning Verify and correct con nections See Section 4 6 6 User I O Connec tion beginning on page 4 15 Outputs of User I O failed Return unit for repair 100 214 239 Rev A Digital Sonifier Interconnect Diagram 7 4 Interconnect Diagram T1 Tins ta ROS IR Rl Export models only r Cordset Variac 5 Line i ex Connector Filter Temperature if m Probe Jack done ay oto onverter Cito al a Ultrasonic Cable is 522 4 fe Power connection Digital Controls Board Hee i i Ez i Sonics J8 12507 pe a rc Display P5 User VO 15 pin Printer 25 pin 100 214 239 Rev A 7 9 Maintenance 7 10 For Your Notes BRANSON 100 214 239 Rev A Digital Sonifier Appendix A APPENDIX A Application Information Operating Considerations The following sections discuss operating techniques under varying conditions Limiting Temperature Rise An important objective in ultrasonic emulsification is to keep processed samples cool Selec tion of the proper processing vessel and cooling bath resolves most heating problems While any type of vessel can be used to hold the sample the shape of the vessel is determined prima cily by the volume to be processed For small volumes choose the smallest diameter vesse
52. cron homogenize immiscible liquids polymerize some materials and depolymerize others 1 2 2 Safety Measures and Guards This manual contains operation instructions for the Digital Sonifier cell disruptor Models 250 and 450 It contains information essential to the proper use and care of this equipment The manual contains notes warnings and cautions These are described as follows NOTE Provides information that the reader should follow to prevent inconvenience CAUTION N Advises the user of a hazard that can cause equipment damage or personal injury WARNING N Alerts the reader to a hazard that can result in severe personal injury Do NOT disregard a WARNING 1 2 3 Safe Operation Setup and Operation instructions are found in Chapter 6 of this manual For safe operation please ensure that all people using this equipment follow those instructions and observe all CAUTION and WARNING notices 1 Make sure that the equipment is properly grounded DO NOT operate if it is not 100 214 239 Digital Sonifier General Precautions 100 214 239 2 The unit is equipped with a three conductor cord and three prong grounding type plug and must be plugged into a three prong grounded receptacle DO NOT under any circumstances remove the power cord ground prong or use a plug Adapter 3 DO NOT operate equipment with the cover removed High voltage is present within the equipment when connected to plant wiring 4 Do not a
53. cs ate applied to your sample by setting the unit to operate in one of several different modes You determine the mode which to operate by specifying parameters for your exper iment There are five system modes as described below Continuous Mode Ultrasonics are applied to the sample until you Stop or Pause the experiment Timed Mode Ultrasonics are applied to the sample for a period of time that you specify by setting the Time parameter Pulse Mode Ultrasonics are turned on and off repeatedly during the experiment You specify the length of the intervals in which ultrasonics are on and off For example you can specify 0 5 seconds for the Pulse On parameter and 2 1 seconds for the Pulse Off parameter You can also specify a time limit using the Time parameter and or a temperature limit using the Temperature parameter Temperature Mode Ultrasonics are applied until the sample reaches a temperature that you specify by setting the Temperature parameter The Temperature Mode requires the optional Temperature Probe and operates as follows when no TIME or PULSE TEMP parameter is entered the experiment stops when the MAX TEMP value has been reached WhenaTIME and MAX TEMP value have been entered the experiment runs until the TIME has elapsed If the MAX TEMP is reached before the TIME parameter has elapsed ultrasonic vibra tions will temporarily stop and the timer will stop counting until the Probe Temperature has dropped by
54. current and Maximum Temperatures are displayed and updated every sec ond Limits You may wish to set a Time or Temperature value as a Limit for some experiments This will cause the experi ment cycle to Stop if either the indicated Time has elapsed or the maximum Temperature was reached Ifa Limit is not desired the parameter should be set to OFF 100 214 239 Rev A 6 3 Operation The following chart summarizes the System Mode options including the use of Limits Continuous Mode Timed Mode Pulse Mode Temperature Mode Pulse Pause Mode Time setting OFF Your Time parameter OFF or Limit OFF or Limit OFF or Limit NOTE 1 You must have a Temperature Probe attached to the unit to use the Temperature Mode the Pulse Pause Mode or to use Temperature as a Limit Pulse On and Pulse Off settings Both OFF Both OFF Pulse On and Pulse Off times Both OFF Optional The system willrecalculate while running the process Max Temp setting OFF OFF or Limit OFF or Limit maximum Temperature parameter Max Temp set to upper temperature for process BRANSON Pulse Temp setting Pulse Temp set to lower temperature for process Cycle Ends upon Stop button or remote command Time parameter elapses Stop button or remote command Maximum Temperature reached Stop button or remote command System States Availability of the Digital Soni
55. e Once you have started entering a value the other Line Select keys and the Page Up and Page Down keys become inactive The Enter key must be pressed to leave the selected parameter When you press a Line Select key for a parameter that is a choice such as Yes No or On Off the available values will be displayed in the menu You select the desired value by pressing the Line Select key next to it and then pressing Enter to accept the value The display then returns to the menu 6 8 100 214 239 Rev A Digital Sonifier Entering System Setup Parameters 6 3 Entering System Setup Parameters At System Setup you can select and store system parameters such as time date language remote operating parameters and printer capabilities The System Setup parameters can only be accessed from the membrane key pad not from the remote terminal and they are not available when a test or experiment is progress To enter System Setup press the Setup function key on the keypad to display the System Setup menu On the menu you will see the default values for each of the System Setup parameters When you are finished with Sys tem Setup exit the Setup menu by pressing the Setup key again The Setup key becomes inactive and the display returns to the Parameter Entry menu The Enter key must be pressed before leaving the Setup menu to store any new parameter value If you change any system parameter you must run at least a portion of a cycle order to
56. e Action Printer does not print a report upon request A Setup parameter has been changed No report data stored in buffer since last report Run atleast one cycle to capture report data Reports not enabled in Setup enable Reports in Setup run at least one cycle to capture new report data Printer is not set to On in Setup Parameters enable Printer in Setup run at least one cycle to capture new report data Printer cable is not con nected or printer is offline Correct printer connec tions and ready status Printer has failed Fix or replace printer Digital Controls have failed Return unit for repair Printer is not compatible Use a compatible dot matrix printer Remote Terminal is not communicating Remote Terminal is not set to On in Setup enable Remote Terminal in Setup run at least one cycle to capture new report data incorrect or faulty RS 232 cable is used verify see Section 4 6 2 Remote Terminal Con nection on page 4 14 Baud Rates do not match between the system and the Remote Terminal set Baud Rate in Setup run at least one cycle to capture new report data Terminal or computer s com munication settings are not set up properly set up the communica tion settings to match the Sonifier s parameters User I O signals are not working correctly User I O is not configured
57. e Pause Mode is activated and running and ensure the process cycle is Stopped before touching the Horn or injury could occur Take this action To get this result PULSEON SEC Use the Up and Down arrow keys if PULSE OFF SEC necessary to display the line showing the Pulse Temp parameter PULSETEMP C PROBE TEMP C PULSE N SEC Press the Line Select key that corresponds BOL SE OLS o4 EE to Pulse Temp PULSE c PROBE TEMP c 6 32 100 214 239 Rev A Digital Sonifier Entering Operation Parameters PULSEON SEC PULSEOFF SEC Use the numeric keys to enter the Pulse Temp PULSE TEMP c See moe melet PROBE TEMP C PULSEON SEC Press the Enter key to store the value PULSE OFF SEC for Pulse Temp or press the Clear key to correct and re enter the value o PULSETEMP __ PROBE TEMP C EB o NOTE 1 The PROBE TEMP value is a display of the temperature of the optional Temperature Probe If the Temperature Probe is not connected the display will show TEMP OFF instead 100 214 239 Rev A 6 33 Operation BRANSON 6 5 Remote Computer or Terminal For yout remote computer terminal to be active you must enable it in the System Setup menu as described in System Setup Remote Computer Terminal on page 6 38 If you want to use only the remote device to control operation of the Digital Sonifi
58. e Treatment Chamber setup adjust as needed Tip is loose or worn out Horn is loose or has failed Tighten or replace defective tip or horn Horn stud is loose or has failed Loose or broken studs must be replaced Replace defective horn Converter cable connection is loose or has failed Tighten connector to Converter Return unit for repair if cable has failed Converter has failed Replace defective Con verter return for repair Microtip is loose or missing Clean horn and install microtip Power supply or controls have failed Return for repair Digital Sonifier operates continuously but does not operate in Pulsed mode Digital controls have failed Return for repair No Temperature reading or inaccurate Tempera ture value displayed Temperature Probe is not connected Plug Probe into unit Temperature Probe has failed Replace Temperature Probe Digital Controls have failed Return unit for repair Slight electric shock when touching a metal part of the system or lab equip ment contacting the sys tem System is not properly grounded Correctelectrical ground to system Cordset has failed or had Ground lead removed replace cordset 100 214 239 Rev A 7 7 Maintenance 7 8 Table 1 System Trouble Analysis Chart BRANSON Symptom Probable Cause Correctiv
59. e are a total of two 2 pages of parameters use the Arrow Up or Arrow Down keys to see more parameter choices on the display Use the number keys to key in a parameter value and press Enter to store the value or accept the choice or press Clear to clear a stored numeric entry or to disable a choice and then press Enter to store the cleared or disabled parameter value The System will not accept parameter values that are out of the range of the system Once the parameters have been changed and stored the experiment is ready to run with the new values 6 4 1 Presets Once you have a set of parameters stored for a given experiment you can save that setup as a Preset Up to 20 Presets may be saved using the Save and Recall functions described in the following sections Presets are saved and recalled using a number 01 to 20 Presets save all parameters for the setup except for the Amplitude param eter Presets ate saved until they are over written or cleared and are maintained memory even if the system is turned off or unplugged 6 4 2 Remote Terminal entry The Remote Terminal may be used to change any of the operation parameters except for Amplitude Instruc tions and commands that you use to enter parameters from the remote terminal are described in Section 6 5 Remote Computer or Terminal beginning on page 6 35 Reviewing Parameters on the Remote Terminal If you have disabled the front panel keypad by sending a KL from the r
60. e probe Refer to Appendix B Optional Items Parts List for specific parts required for the optional items and to Appendix C Accessories Parts List for parts needed for the available accessories 100 214 239 Rev A 4 1 Installation and Setup BRANSON 4 2 System Block Diagram The block diagram shows the relative interaction of the components of a Digital Sonifier sys tem The items inside the bold outline are found in the Digital Sonifier unit Some elements shown ate optional Ultrasonic Power Supply Module Remote Terminal Converter and Horn 4 3 System Component Description 4 3 1 Standard Components The standard system components are Sonifier unit Power cord Converter Horn and tips 4 2 100 214 239 Rev A Digital Sonifier System Component Description 4 3 2 Optional Items A complete list of optional items 1s provided in Appendix C of this Guide Printer Epson FX compatible dot matrix printer with a parallel port interface with a DB25M cable connector only Remote Terminal or Computer Any user provided computer or terminal that allows ASCII data to be transferred over an RS 232 serial interface The pin out of the serial connector is listed in Hemote Terminal Connection on page 4 14 The computer interface software and setup are for the reader to determine Temperature Probe Omega Technologies model OL 703 PP 0 125 inches in diameter and 4 5 inches in le
61. ector and turn the printer on The Printer must be Set Up in order to function see page 6 18 4 14 100 214 239 Rev A Digital Sonifier Electrical Connections to Equipment 4 6 5 Temperature Probe Connection The temperature probe is connected to the Digital Sonifier using a 1 4 inch RCA type phone jack connector The Omega Temperature Probe that 1s specified matches properly and is the only temperature device for use with the Digital Sonifier The Probe temperature is displayed example on page 6 32 or the display will shown TEMP OFF if no probe is connected 4 6 6 User I O Connection The Digital Sonifier is equipped with a standard external connection to allow you to design and connect your own custom interface for controlling the unit The User I O interface can be use ful when you need to activate the Sonifier remotely for example when the operator must start and stop the unit from another room for safety reasons User I O Pin Out DB15F provided for customer designed interface DB15F Pin Function Signal or Use E eS SM OT 2 Signal Ground for Amplitude Signal Ground 3 Signal Ground for Power Signal Ground 4 Signal Ground for Temperature Signal Ground 5 Amplitude Signal Output 0 10VDC 0 100 Amplitude 6 5 VDC Return 5 VDC Ground 7 not used no connection 8 not used no connection 9 Conto ian oben itt loop 5VDC signal drops to
62. ed in 15 minutes and a good yield of the enzyme Xanthine dehydrogenase extracted Mineral rock excellent for cleaning surfaces between polishing stages Mitochondria separate from cells without injury Mitochondria themselves can be broken with longer ultrasonic treatment Inner membrane subunits also isolated Muscle tissue 1 homogenized in 4 minutes heart muscle in 6 minutes Mycobacteria a 20 ml growing media completely disrupts in 14 minutes Clumps break quickly An immunological compound prepared Mycoplasma antibody a suspension of Campo W cells treated for 5 minutes gave 12 lines with the sera in a gel diffusion test The extract was estimated to contain 12 75 mg protein per ml by Blaret reaction Myeloma tumor cells complete disruption in 10 minutes 30 disruption in 2 minutes Myleran made colloidal suspension and dissolved in approximately 1 minute N crassa nuclease was isolated and purified from conidial extracts after 5 minutes treatment Naegleri gruberi this free living soil amoeba was treated ultrasonically to release subcellular infectious material Neurospora 40 ml 4 minutes produced more protein than freeze thawing for study of enzymatic synthesis of cystathionine Nocardia ostenodes breaks clumps and disintegrates in less than 10 minutes Nucleoprotein extracted from tissue May be degraded selectively Oil and water emulsions permanent stable emulsions in a few seconds Particle size redu
63. emote terminal only the Start Stop and Pause keys are active If both the keypad and the remote computer or terminal are active the parameters displayed on the front panel will be similar to those described in this section but any values you enter on the keypad will be updated at the remote computer or terminal only when you request them by issuing the Recall Parameters IRP command See Section 6 5 Remote Computer or Terminal beginning on page 6 35 for more infor mation 6 24 100 214 239 Rev A Digital Sonifier Entering Operation Parameters The Operation parameters that you can set from the keypad are described in the following table Detailed steps for each parameter are described in the pages that follow Table 6 2 Operation Parameters Parameter Description Valid Entries When you view a set of parameters that was saved using the Save function Preset ID contains the preset identification number under which the parameters were saved The number is not available for entry The Preset ID will be followed by an asterisk if you change any of the parameters Preset ID 01 20 The length of time the experiment should run in hours 0 00 01 9 59 59 minutes and seconds Time This parameter is available only if a temperature probe is connected to the system The temperature units Fahrenheit or Celsius are determined by the setting of 32 0 212 0 F the Temperature parameter selected during System
64. ent breaks particles down to almost molecular size Rhodopseudomonis palustris complete disruption 1n 4 minutes Rhodospirillum rubrum excellent disruption in a few seconds Rimosus monocellular elements from surface grown colonies obtained in 1 minute Complete disruption in 5 minutes 50 disruption in 2 minutes RNA rapid and thorough re suspension of 9 T CA pellets during extractions Rocks excellent for disaggregation of sedimentary rock and for cleaning material rock sur faces between polishing stages S faecalis excellent disruption in 1 minute S fragilis 5 minutes yielded excellent release of galactokinase more than any other method Subcellular particles may be extracted or disrupted Saliva glands complete disruption Salmonella various culture media or phosphate buffered saline disintegrated between 40 and 50 in 10 20 minutes Sonifying was one step in an improved assay for enzyme thiogalactosize transacetylase Salmonella typhimurium and enteritidis bacteria were suspended in 1 300 volume of orig imal culture sonified for 4 minutes and centrifuged for 20 minutes at 20 000 g Extracts were found to catalyze the synthesis of cytidine diphosphate 3 6 dideozyhexoses Scholasticism mansion complete disruption Sedimentary rock completely disperses flocs with the release of all bound silt and clay particles Sediments ultrasonic treatment disperses fine material permitting quick neat separation of sand f
65. er you can lock out the Sonifier s keypad by issuing a command from the terminal as described in Table 6 3 Remote Terminal Commands on page 6 36 You can enter Operation parameters through the remote computer or terminal but you must use the keypad to enter System Setup parameters You can use your terminal or computer to direct the system to start stop ot pause the experiment to send a report to the printer or terminal or to display the current parameters NOTE 1 To quickly exit from the Remote Terminal Mode turn the Sonifier s power switch off and then back on The system defaults to the front panel control mode when it is first powered up The commands that you can issue from the remote computer or terminal are described in Table 6 3 Remote Terminal Commands on page 6 36 Use your remote computer or terminal s keypad to key in alphanumeric values and use your remote computer or terminal s Enter or Return key to store the values All remote terminal com mands begin with an exclamation point Refer to Table 6 2 Operation Parameters on page 6 26 for valid formats and limits for the parameters To Enter a parameter value using a Remote Computer or Terminal Step Action Type the remote terminal command that corresponds to the parameter whose value 1 you want to change Remote terminal commands are described in Table 6 3 Remote Terminal Commands on page 6 36 The remote computer or terminal displays
66. eration has been paused and the front panel Pause LED will be lit The Start Stop LED also remains lit while an experiment is paused To resume operation press the Pause key again or send another command 6 42 100 214 239 Rev A Digital Sonifier Displays During the Experiment Keypad Enabled If the keypad is enabled the Up and Down arrows Start Stop and Pause keys are active and the display shows the information described 1n the following table Table 6 4 Data Displayed During the Experiment when the Keypad is Enabled Display Description RUN TIME The elapsed time of the experiment increasing from 0 00 00 PROBE TEMP The temperature currently being read by the temperature probe if one is connected If you entered Pulse On and Pulse Off times shows PULSE ON when PULSE ON and PULSE OFF ultrasonics are active and PULSE OFF when ultrasonics are inactive The current percentage of maximum power being used represented as Power display bargraph a bar graph Displayed values are approximate using 5 segments The maximum temperature that will be reached during the experiment MATEM if you specified a Maximum Temperature parameter PULSE TEMP In Pulse Pause mode the temperature at which the system will begin calculating the Pulse Pause ratio TIME The time limit for the experiment if one was entered POWER The power in watts being consumed by the Horn at any moment Dur ing a PULSE
67. f maximum amplitude microtip 7096 maximum Prevent excessive temperature increase in the sample by setting ultrasonics to operate in Pulse mode or in Pulse Pause mode Bringa sample to a desired temperature and hold it there varying by only a few degrees for a desired duration using the Pulse Pause mode Set the maximum allowable temperature in the sample so that ultrasonics will stop automatically when the specified temperature is reached 100 214 239 Rev A Digital Sonifier Controls and Commands 2 2 Controls and Commands This section describes the controls and commands that you use to operate the Digital Sonifier A detailed description of how and when to use each front panel control the valid formats for the data that you enter and the response you receive from the system when you use each of these controls 1s provided in Chapter 6 Operations The Digital Sonifier is equipped with a keypad and LCD display on the front panel of the unit With the keypad you can set functional modes of operation and input digital parameters Avail ability of the various functions depends on the mode or state of the system If you attempt to use a function that 1s not available you will be alerted by a beep Figure 2 2 Digital Sonifier front panel Controls BRANSON Digital Sonifier MODEL 450 SONIFIER 5 COPYRIGHT 1997 m 0 10 20 30 40 50 60 70 80 90100 s 5 Print Start 100 214 239 Rev A 2 3 Introduction
68. fier s functions depends on the system state The normal state of the system for most users 1s the Run or Ready mode Some remote terminal commands are noted where appropriate com mands have the format of an followed by upper case letter or letters i e TS for the Test command These commands are also summarized in the Remote Terminal section found on page 6 35 T he possible system states eu include Power Up The system enters the Power Up state when the power switch is turned on The fan starts to run and the system then enters the Self Diagnostics state Self Diagnostics In this state the system performs hardware and software checks on itself The front panel display shows several diagnostic displays indicating which step in the self diagnostics it is in and the front panel LEDs will illuminate as a visible test After it successfully completes this system check it enters the Ready state Ready In the Ready state the system is waiting to begin an experiment and it can accept parameter settings that you enter through the keypad or the remote computer or terminal The front panel display 6 4 100 214 239 Rev A Digital Sonifier Front Panel Controls shows the Amplitude setting and some parameters and the appropriate LEDs are illuminated The sys tem must be in the Ready state for you to start a new experiment or make changes to any parameters Run After setting parameters for your application pressing the Sta
69. g the Page Up arrow 15 active and its LED is turned on If there are menu items below the page you are viewing the Page Down arrow 1s active and its LED 1s turned on Start Stop Key ON and OFF Commands The Start Stop key and ION command are active during Parameter Entry mode except when you are changing a parameter value and during the Sonics Active displays When you press the Start Stop key or type in the ON Command the experiment is executed and the LED next to the key is turned on When you press the Start Stop key again or type in the OFF com mand the experiment 1s stopped and the LED 1s turned off Setup Key The Setup key is active during Parameter Entry mode except while you are changing a param etet value and while you are viewing the Setup Menu When you press the Setup key the first page of the Setup menu is displayed and the Setup key s LED 1s turned on When you press the Setup key again the Setup menu is closed and its LED 1s turned off Test Key TS Command The Test key and the TS command are active when the system is in the Ready state When you press the Test key or type in the TS command ultrasonics are enabled for two seconds turn on ultrasonics for another two seconds you can press the Test key or type in the TS com mand again 100 214 239 Rev A 2 5 Introduction to the Digital Sonifier BRANSON 2 6 Pause Key Command The Pause key and the H command are active only when the u
70. g this attachment will disrupt Continuous most cells with the exception of the more diffi Flow cult types Materials being treated may be 100 146 171 Attachment passed through the attachment more than once to obtain the desired results A water jacket and input output and overflow connec tions are provided For use with horns having outside threads C 1 100 214 239 Rev A Accessories Parts List BRANSON Item Description Branson EDP Number Continuous Flow Glass Rosett Cooling Cell Cooling cell for continuous circulation of the substance being processed The cell is equipped with intake and output connections for continuous processing and a double cham ber for cooling Normally adequate cooling is achieved by connection to the cold water tap or by using a closed circuit system An ice salt water solution will maintain a temperature below 0 Borosilicate glass construction allows observation during treatment Not suited for difficult cells 201 123 004 Sealed Atmosphere Treatment Chambers Used for batch treatment of infectious materials Input and output connections allow filling and emptying without breaking the air tight seal and permit processing with an inert gas Stainless steel 9 10 ml 101 021 001 e 6 15 ml 101 021 002 25 50 ml 101 021 003 Same as above 101 021 001 series but with cooling water jacket 3 10 ml 101 021 004 e 6 15 ml 1
71. gether can cause the system to operate ina temperature limiting fashion which can cause ultrasonics to be suspended briefly and then reactivate to continue a process cycle without warning Do not handle the Horn or Converter if using MAX TEMP and TIME in the Temperature Mode and ensure the process cycle is Stopped before touching the Horn or injury could occur Take this action To get this result necessary to display the line showing the MAX TEMP __ Time parameter AMPLITUDE ___ PRESET 1 __ TIME _ __ __ hr m s Use the Up and Down arrow keys if n 100 PRESET 10 __ TIME hr m s Press the Line Select key that corresponds MAX TEMP to Time C AMPLITUDE ___ 6 26 100 214 239 Rev A Digital Sonifier Entering Operation Parameters PRESET 1 _ 7 8 9 Use the numeric keys to enter a value TIME hrm 6 for Time how long you want the experi a ment to run PESE AMPLITUDE ___ 0 0 100 PRESET 10 __ Press the Enter key to store the value TIME _ __ __ hrim s for Time or press the Clear key to re enter __ _ or the time AMPLITUDE ___ Clear 0 100 NOTE 1 If using the Time parameter as a limit in Pulsed Mode the elapsed time will be greater than the Time parameter value since the Time value is the duration that ultrasonic vibrations are active the Pulse On
72. gher amplitudes Tissue Disruptor Designed for disintegration of difficult tissues this stainless steel accessory has a specially con structed cell bottom that holds up to six grams of tissue A water jacket may be provided for cooling 100 214 239 Rev A Digital Sonifier System Component Description Cup Horn Cup Horn Cavity pco E Inlet Outlet Cup Horns allow materials to be treated in small vials or test tubes without immersing the ultrasonic horn or micro tip in the material thereby providing completely sterile conditions A Cup Horn 1s attached directly to the converter and the assembly 1s mounted upside down on the lab stand with the Cup Horn at the top With chilled water the Cup Horn test tubes are suspended in the Cup with the contents of the tubes just below the water level Ultrasonic energy is then conducted from the surface of the horn through the water and test tube walls to the contents of the tube Some energy loss may occur when applying ultrasonics in this indirect way and processing can take longer than if the ultrasonic horn were immersed directly in the solution There are two types of Cup Horn a high intensity unit that will accommodate a single test tube and a larger unit for multiple test tubes The high intensity Cup Horn has a concave bottom that focuses the ultrasonic energy on the bottom of the tube The larger unit with a dia
73. ghts LANGUAGE ENGLISH TEMPERATURE F 0 100 PRINTER OFF Use the Up and Down arrow keys if REMOTE OFF necessary to display the line showing the LANGUAGE ENGLISH Language parameter TEMPERATURE F 0 100 PRINTER OFF Press the Line Select key that corresponds REMOTE OFF to Language LANGUAGE ENGLISH TEMPERATURE F 6 10 100 214 239 Rev A Digital Sonifier Entering System Setup Parameters Press the same Line Select key again to toggle among the possible values for Language English Spanish German Italian or French PRINTER OFF REMOTE OFF LANGUAGE ENGLISH TEMPERATURE F Press the Enter key to store the choice for Language PRINTER OFF REMOTE OFF LANGUAGE ENGLISH TEMPERATURE F 100 Press the Setup key to exit the System Setup menu The Setup LED extinguishes 100 214 239 Rev A 6 11 BRANSON Operation System Setup Date The Date parameter affects the date shown on the display and printed on the Reports Take this action To get this result PRINTER OFF REMOTE OFF Press the Setup key to display the System Setup menu Its LED lights LANGUAGE ENGLISH TEMPERATURE F 100 TIME OF DAY 17 00 Use the Up and Down arrow keys if DATE 01 01 1997 necessary to display the line showing the Date parameter TIME OF DAY 17 00 DATE 01 01 1997 Press the Line Select key that correspo
74. hoices when the Printer parameter 1s enabled Take this action To get this result PRINTER OFF REMOTE OFF Press the Setup key to display the System Setup menu Its LED lights LANGUAGE ENGLISH TEMPERATURE F 100 MODEL PANASONIC Use the Up and Down arrow keys if necessary to display the line showing the Model parameter 100 MODEL PANASONIC Press the Line Select key that corresponds to Model 6 20 100 214 239 Rev A Digital Sonifier Entering System Setup Parameters MODEL PANASONIC Press the same Line Select key again to toggle among the possible values for your printer Model Panasonic Epson or IBM MODEL PANASONIC Press the Enter key to store the value for Model 0 100 Press the Setup key to exit the System Setup menu The Setup LED extinguishes 100 214 239 Rev A 6 21 Operation BRANSON System Setup Reports The Reports parameter enables or disables the Reports option to your Printer and or your Remote Computer ot Terminal Take this action To get this result Press the Setup key to display the System Setup menu Its LED lights PRINTER OFF REMOTE OFF LANGUAGE ENGLISH TEMPERATURE F 0 100 Use the Up and Down arrow keys if necessary to display the line showing the Reports parameter BAUD RATE 9600 REPORTS DISABLED DATA BITS 8 PARITY HOHE 0 100 Press the Line
75. ich 1s screwed onto the disruptor horn permits continuous processing of low viscosity materials at rates of up to 38 liters per hour Designed primarily for emulsifying dispersing and homogenizing the attachment will disrupt most cells with the exception of the more difficult species The materials being treated can be passed through the attachment more than once to obtain desired results For cooling a water jacket with input output and overflow connections 1s provided Rosett Cell The Rosett Cell made of borosilicate glass has a conical shape with three side arms through which the solution 1s driven by pressure produced from vibrations from the ultrasonic horn thereby exposing the substance to ultrasonic energy repeatedly during circulation When the Rosett Cell is immersed 1n a cooling bath the enlarged glass surface area and circulation through the side arms provide an efficient means of heat exchange The Rosett Cell is available in three sizes 8 25 ml 25 180 ml and 35 300 ml Flow Thru Rosett Cell The Flow Thru Rosett Cell is equipped with its own water cooling Jacket with intake and out put connections for continuous processing and a double chamber for cooling Normally ade quate cooling can be achieved by connection to the cold water tap or by using a closed circuit system An ice salt water solution will maintain a temperature below 0 C Since the double chamber is made of glass the substance can be easily observed du
76. id A limited warranty as specified may apply to certain components of the equipment WARRANTY EXCEPTIONS This warranty shall not apply to equipment subjected to misuse improper installa tion alteration neglect accident or improper repair This warranty is limited to the original purchaser and is not transferable Horns and tips fabricated by Branson for use in equipment described in this manual are manufactured to exacting parameters Using altered or modified horns and tips or horns and tips otherwise unqualified by Branson can produce undue stresses that may damage the equipment Equipment failures resulting from using unquali fied horns and tips are not covered by the Branson warranty Microtips are designed to give maximum mechanical energy output Since they operate close to the stress limits of titanium Branson cannot guarantee microtips against failure CONTACT YOUR BRANSON REPRESENTATIVE OR BRANSON ULTRASON ICS CORPORATION DANBURY CONNECTICUT SHOULD YOU HAVE ANY QUESTIONS CONCERNING HORN QUALIFICATION 100 214 239 Digital Sonifier How to Contact Branson 1 4 How to Contact Branson The mailing address and telephone information for Branson is as follows Branson Ultrasonic SA 9 chemin Faubourg de Cruseilles P O Box 1031 CH 1227 Carouge Geneva Phone 41 22 304 83 58 Fax 41 22 304 83 59 Tell the operator which product you have and which person or department you need If after hours please leave a voice
77. ied Thursday August 23 2001 10 52 AM Digital Sonifier 100 214 239 E effects of ultrasonic irradiation on biological materials A 4 electrical connections to equipment 4 10 entering operation parameters 6 23 entering parameters from the keypad 6 23 entering system setup parameters 6 3 equipment assembling 4 6 loge E 6 41 experiment displays during 6 40 feat t s E 2 7 flowsthiu s iii erii erai e ERR ERE inert 4 4 flow thru Rosett 4 5 taming o ton eI ere A 3 front panel Contios esaten Ega ie bete been 6 3 G glass powdoer ies ini ep e A 4 gloSsalty coe eene ee Plenus 2 7 guards and safety 4 11 H horn connecting tip to 4 8 horns CONNMOCTING EE 4 7 how to contact 1 6 input power 4 10 installation and 4 1 installation checklist sese 4 1 Intended use ener 1 1 Introduction iecore noe ARA cre oe tendra 2 1 CONFIDENTIAL For Review Only Index
78. ier is used to verify that the unit is func tioning providing ultrasonic energy to the Converter and Horn Later you can run another test on the system for your particular experiment described in System Performance Benchmark on page 5 3 Before testing the Sonifier always make sure that the horn is not touching anything The Sys tem also performs several self tests when 1t 1s first turned on Step Do this To obtain this result Set up the Digital Sonifier following the instructions in this manual If no horn pe ok Prepare the Sonifier to operate if it l IS currently installed was not previously assembled Mount a 1 2 Disruptor horn with a P y flat tip if tapped to the Converter After you have connected the Con Nery thatthe System passos alts self tests observing that there are verter Horn to the Converter Cable x no error messages the front panel 2 verified all other connections are as display Gaited The Digital Sonifier advances to the Turn the Unit On and observe the A h h self test displays eady mode and shows the norma Ready display see page 2 4 Adjust the Amplitude control to Ensures that ultrasonic energy will be at some mid range value and will 3 approximately 50 observe the if value on the front panel display not Cana Gamage it wera Using a microtip must be less than 70 Verify that the Horn is not touching VENES bs ioc d pe anything T ou id eara
79. igital Sonifier is to be used for remote operation ensure that the unit is situated within full view of the operator to prevent injury or equipment damage through an accidental or automatic start up 1 3 Regulatory Compliance The Digital Sonifier is designed for compliance with the following regulatory guide lines European norms EN 61010 1 EN55011 EN50082 1 ENV50140 ENV50141 EC 529 IP 65 water resistant for Membrane Keypad The Digital Sonifier is CE compliant 1 3 Safety and Support BRANSON 1 3 Warranty Refer to the Terms and Conditions of Sale found on the back of your Invoice for information about the product Warranty issued of your Branson products If you have any questions please contact your Branson representative The product war ranty information is summarized below WARRANTY When used in accordance with written instructions and under normal operating conditions Branson Ultrasonics Corp equipment is guaranteed to be free of defects in MATERIAL and WORKMANSHIP for two 2 years from the date of orig inal delivery by BRANSON or by an authorized representative Any unit which proves defective during the stated period will be repaired free of charge or replaced at the sole discretion of Branson Ultrasonic S A 9 ch Faubourg de Cruseilles CH 1227 Carouge Geneva or an authorized repair station as advised by BRAN SON provided the defective unit is returned properly packed with all transportation charges prepa
80. in 290 Ibs 33 Nm 100 098 120 1 2 20 x 11 4 in 450 Ibs 51 Nm 100 098 370 1 2 20 x 11 2 in 450 105 51 Nm 100 098 123 After the Stud has been reinserted you can reassemble the Horn to the Converter Follow the same procedure as found in the Installation section of this manual See Section 4 4 Assembling the Equipment beginning on page 4 9 7 3 Troubleshooting Charts Use the following troubleshooting charts for possible problem conditions and resolutions The charts are based on the assumption that the proper setup and operation instructions have been followed and or the system was working and then developed a problem 100 214 239 Rev A 7 5 Maintenance Table 1 System Trouble Analysis Chart BRANSON Symptom Probable Cause Corrective Action Main power fuse fails or circuit breaker trips when System is plugged into electrical outlet Cordset has failed Replace cordset Power Switch has failed Line filter has failed Return for repair Display screen does not appear when unit is turned on Fan does not operate System is unplugged or main power is missing Correct power problem Unit fuse has blown it should not under normal con ditions Replace fuse Cordset has failed Replace cordset Power Switch has failed Line filter has failed Unit failed due to connection to incorrect input voltage Return for repair
81. isruption in 3 minutes Vibrio comma excellent disruption Vibrio extraction excellent for experimental vaccines Evidence of breakage of virus anti body bonds Virus can be extracted without damage at low power or broken at high power Vitamin E 30 seconds of ultrasonic treatment put material in solution with a resultant permanent suspension W138 virus cell free V 2 virus obtained 30 seconds using 6 ml of Veronal buffer with W138 cells containing V 2 virus Yeast complete disruption in 3 10 minutes Complete disruption of 9 grams pressed yeast 18 ml buffer in 8 minutes Protein release 52 mg ml from an aged sample 100 214 239 Rev A Digital Sonifier Appendix B APPENDIX B Parts Lists Replacement Parts Item Description EDP Number 3 15 Amp for Model 250 117V or 200 245V Replacement Fuses 5 00 Amp for Model 450 117V 200 049 121 2 00 Amp for Model 450 200 245V North American 117VAC 5 15R 200 030 012 Cordsets F Harmonized European 200 245 000 087 062 Silicon Grease Stopcock Grease silicone 101 053 002 Stud Replacement adaptor Stud 100 098 249 horn to converter Converter 102C Converter CE compliant 101 135 022 For use with Branson Sonifier Cell Dis ruptors Wrenches gt Spanner 201 118 019 Open end for 1 2 disruptor tip 201 118 010 Extends 1 2 diameter disruptor horn 1 2 Wave HornExtension an additional 1 2 wavele
82. l Englishundex fm modified Thursday August 23 2001 10 52 AM Digital Sonifier Index W WAMING E eR qu 6 41 ai aa Rc e Ee dd 1 4 warranty 1 3 workplace setting UP uei eR Hee Cete cer bd 1 2 100 214 239 CONFIDENTIAL For Review Only 1 9 2nd Review copyfilename C Documents and Settings St phanie My Documents CD ROM Sonifier Manuals Digital Englishindex fm modified Thursday August 23 Index BRANSON 1 10 CONFIDENTIAL For Review Only 100 214 239 filename C Documents and Settings St phanie My Documents CD ROM Sonifier Manuals Digital Englishundex fm modified Thursday August 23 2001 10 52 AM
83. l that allows the probe to be inserted without touching the sides of the vessel This minimized diam etet raises the height of the liquid exposing more surface area to the cooling bath for more effective heat transfer Based on heat transfer characteristics the following vessel materials are recommended listed in decreasing order of heat conductivity 1 Aluminum Stainless steel Thin wall glass Thick wall glass Plastic NOTE 1 Plastic vessels are not recommended unless the sample being processed will be unaffected by heat or unless ultrasonic treatment is pulsed Immersing the processing vessel in a simple ice water bath 0 C provides sufficient cooling for larger sample volumes if required treatment times are short If temperature rise is too great with this method consider using the following alternative baths lce salt 6 C lce alcohol 14 C Dry ice alcohol water 30 to 40 C 100 214 239 Rev A A 1 Application Information BRANSON A 2 NOTE 1 All baths need a magnetic stirrer For smaller volumes with less than 30 seconds treatment time an ice water bath is sufficient For longer periods especially when high power is required a lower temperature bath is required Vessel Capacity and Speed of Temperature Rise The smaller the volume the more difficult the cooling procedure becomes For example using any given power input to treat 5 ml for a long period would require
84. llow the horn or microtip to contact lab stands beakers etc or horn microtip failure may result 5 Establish a standard of operation and periodically test the equipment as described in Ultrasonic Test on page 4 17 Although the Digital Sonifier operates outside the normal range of human hearing some applications can create audible noise above 85dB Noise can occur for example when the device touches a solid material or when its operation causes nearby objects in the lab to vibrate If an uncomfortable level of noise is present the operator should wear ear protection for safe operation Appropriate eye protection should be worn when operating the Digital Sonifier to prevent possible splash injury originating in the solution WARNING N Never touch the horn or tip when ultrasonics are active Touching the horn or tip while the unit is on can result in serious injury When you handle remove or attach a horn or tip always make sure that the ON OFF switch is OFF 1 2 4 Setting Up the Workplace The unit should be positioned away from radiators and heating vents A fan inside the unit maintains a safe operating temperature in the power supply by circulating air over the components Therefore place the unit so that the air intake on the bot tom of the power supply is not blocked Periodically unplug the unit and clean the air intake underneath the power supply to ensure that dust or dirt is not restricting the flow of air If the D
85. lt including 1 2 horn 101909996 Kit Digital Sonifier Model Digital Sonifier 450 system kit 101 063 591 450 200 245V 1 2 Horn 200 245 volt including 1 2 horn Kit Digital Sonifier Model Digital Sonifier 450 system kit 101 063 590 450 117V 3 4 Horn 117 volt including 3 4 horn Kit Digital Sonifier Model Digital Sonifier 450 system kit 101 063 593 450 200 245V 3 4 Horn 200 245 volt including 3 4 horn 100 214 239 Rev A Digital Sonifier Appendix C APPENDIX C Accessories Parts List uus Branson EDP Item Description Number 1 2 diameter stepped w grad scale 101 147 036 1 2 diameter stepped tapped 101 147 037 1 2 diameter stepped solid 101 147 038 3 8 diameter stepped solid 101 147 039 Disruptor 1 2 diameter exponential tapped 101 147 040 Horns 1 2 diameter exponential solid 101 147 041 1 2 diameter catenoidal solid 101 147 042 3 4 diameter stepped solid 101 147 043 1 diameter stepped solid 101 147 044 9 4 diameter solid high gain 101 147 035 Permits material to be treated while isolated in smalltesttubes Cups have transparent plastic bodies Horn is attached to converter and Cup Horns mounted upside down 2 diameter 101 147 047 9 diameter 101 147 048 Permits continuous processing of low viscosity materials with rates up to 38 liters hour Designed primarily for emulsifying dispersing and homogenizin
86. ly moving cells or parti cles pass the face of the tip many times during the process When you treat a solid piece how ever the energy pattern from the tip of the horn has a tendency to repel the solid away from the tip The solid does not receive treatment but simply spins or circulates around the con tainer You can effectively treat all but the most difficult materials by following these two steps 1 Homogenize the tissues or solids by placing them in a high speed blender with the solution 2 Insert the horn in the liquid sample for complete disruption If you must disrupt solid pieces especially those that are extremely resistant to breakage with out homogenizing them place the horn directly over the tissue ot right against it Using Glass Powders with Solution To disrupt difficult cells and tissues adding glass powders 5 microns to 0 5 mm will materially decrease treatment times especially when used in conjunction with the standard high intensity A ratio of 1 part glass powder to 2 parts liquid 1s recommended 100 214 239 Rev A Digital Sonifier Appendix A The Effects of Ultrasonic Irradiation on Various Biological Materials Actinomyces 3 minutes of sonifying produces excellent disruption with 50 protein release and excellent enzyme activity Actinomycin D suspended in 3 minutes Aerobacter aerogenes excellent breakage with better enzyme release than any other method A low power setting ca
87. message with your name and return tele phone number 1 4 1 Before Calling Branson for Assistance This manual provides information for troubleshooting and resolving problems that could occur with the equipment see Chapter 7 If you still require assistance Branson Product Support is here to help you The following questionnaire lists the common questions you will be asked when you contact the Product Support department to help identify the problem Before calling determine the following information 1 Your company name and location 2 Your return telephone number 3 Have your manual with you If troubleshooting a problem refer to Chapter 7 4 Know your equipment model and serial numbers found on a gray data label on the units Information about the Horn part number gain etc or other tooling may be etched into the tooling Software or firmware based systems may provide a BOS or software ver sion number which may be required The Digital Sonifier provides the firmware information on the start up screen 5 What tooling horn and booster are being used 6 What are the setup parameters and mode 7 ls your equipment in an automated system If so what is supplying the start signal 8 Describe the problem provide as much detail as possible For example is the problem intermittent How often does it occur How long before it occurs if you are just powering up If an error is occurring which error give error number o
88. meter of two or three inches allows the immersion of multiple test tubes The larger Cup Horns have clear plastic walls which permit easy viewing of the activity in the tubes during processing Both types of Cup Horn are designed to allow chilled water to circulate through the cup to prevent heating of the solution as a result of the ultrasonic activity CAUTION The bottom of the test tubes should not be in contact with the surface of the ultrasonic horn Such contact could cause breakage and loss of sample 100 214 239 Rev A 4 5 Installation and Setup BRANSON Flow Thru Horn Stud Flow Thru Inlet outlet hollow core The Flow Thru Horn has two inputs or orifices at the non vibrating nodal point of the horn A pre mixed substance is fed through one of the inputs while the horn 1s ultrasonically acti vated Because two inputs are available two different types of material can be treated simulta neously for mixing or emulsifying The processed solution exits at the tip of the horn The horn can be used as a continuous flow device to collect the solution in one large vessel Continuous Flow Attachment Opening at Top is internally threaded C EE Inlets Outlets Flow Through Attachment ee Attachment is iE hollowed for use with Disruptor horn 4 6 100 214 239 Rev A Digital Sonifier System Component Description This attachment wh
89. n release sulfatase activity into the supernate with no obvious disruption of the majority of cells Aerobacter suboxydans excellent breakage but requires higher power than A aerogenes Algae secendesmus 10 ml concentrated solution completely disrupts in 1 minute Alkaloids total amount and speed of extraction are greater than with standard methods Extraction from ipecac root in 30 seconds yielded more alkaloid than Soxhlet extraction in 5 houts Antibioticus monocellular elements from surface grown colonies obtained in 1 minute Complete disruption 5 minutes 50 disruption in 2 minutes Antigen the Sonifer Cell Disruptor is used extensively to produce antigens and vaccines It can increase yield or expose otherwise unobtainable sites Aorta 1 gram disintegrates in 2 minutes Aphanomyces after blending complete disruption in 3 minutes Arthobacter tumescens 10 gm in 40 ml in 5 minutes for O coumaric reductose Ascaris eggs 8 ml concentrated solution completely disrupts in 4 minutes Asperigillus complete disruption in 4 minutes Aurefaciens monocellular elements from surface grown colonies obtained in 1 minute Com plete disruption in 5 minutes 50 disruption in 2 minutes Azotobacter vinelandii 15 ml buffered solution 200 mg wet wt ml completely disintegrates in 2 minutes B anthracis 80 disruption of anthracis in 4 minutes Complete disruption of 10 ml of eryisipelothrix rhusipathiae in 10 minutes B cere
90. n the carton and locate the packing list Carefully unpack the components and check them against the packing list Save all packing materials in case the equipment needs to be shipped Inspect the components for any damage that may have occurred during shipping Toce Report all shipping damage to your carrier 100 214 239 Rev A 3 1 Delivery and Handling 3 2 For Your Notes BRANSON 100 214 239 Rev A Digital Sonifier 4 Installation Checklist Installation and Setup 4 1 4 2 4 3 4 4 4 5 4 6 4 7 4 8 Installation Checklist 4 1 System Block Diagram 4 2 System Component Description 4 2 Assembling the Equipment 4 9 Input Power Requirements 4 13 Electrical Connections to Equipment 4 13 Guards Safety Equipment 4 17 Ultrasonic Test o 4 18 4 1 Installation Checklist To install the Digital Sonifier you will need these items Digital Sonifier unit Power cordset Converter Horn and horn tips if applicable Spanner wrench can be ordered from Branson refer to Appendix C Open ended wrench can be ordered from Branson refer to Appendix C Any accessories shipped with the unit Any customer supplied items i e computer printer and temperatur
91. nd Stop Abort When you press the Start Stop button or issue an OFF command the system enters the Stop Abort state The experiment or test is stopped 6 2 Front Panel Controls The membrane keypad on the front panel of the Digital Sonifier allows you to enter parameters for both System Setup and Operation of the unit during a test or experiment You can also use a remote computer or terminal to enter operation parameters If a remote computer or terminal is enabled you can lock out the keypad on the front panel of the unit if you want to operate the unit only from the remote computer or terminal When the keypad is disabled through the remote computer or terminal all keys except the Start Stop key and the Pause key are disabled There are two additional controls Amplitude Control and Power Switch which work with the front panel con trols but are not actually part of the front panel The remote computer or terminal may be used to control the system as well 6 2 1 Power Switch The Power switch for the system is located on the front of the unit underneath the front panel controls It is a simple rocker switch on off operation When turned on the front panel LEDs will light during the power up sequence and the unit s fan will run 100 214 239 Rev A 6 5 Operation BRANSON 6 2 2 Temperature Probe The optional Temperature Probe is required for the Temperature Mode the Pulse Pause Mode ot to use Tem perature as a Limit for
92. ndex fm modified Thursday August 23 Index l 6 recall ees 6 36 remedy and liability limitation OF 1 6 remote 6 7 remote termirial manae Ilis 6 37 remote terminal 2 6 remote terminal connection 4 10 reports printing and sending eee 6 39 o T EHE 6 19 requirement 5 1 Ud 1 3 RFI guidelines compliance a 1 3 MES fol Bor 1 3 Rose tt cell oe ite mte oe teen ru tete 4 5 CONFIDENTIAL For Review Only BRANSON 100 214 239 filename C Documents and Settings St phanie My Documents CD ROM Sonifier Manuals Digital Englishundex fm modified Thursday August 23 2001 10 52 AM Digital Sonifier Index S Safety ciet t m RR 1 1 safety 1 1 safety equipment 4 11 safety measures ssssesssssseeeeeeeen mener 1 2 sample discoloration uiui idee creer A 3 save and recall presets 6 36 SAVE etae edes 6 36 6 39 setting up the
93. nds to Date 6 12 100 214 239 Rev A Digital Sonifier Entering System Setup Parameters Use the numeric keys to enter the current date using an American or European date format as appropriate If you set English as the Language parameter the system uses the American date format MM DD Y YY Y Otherwise it uses the European format DD MMy Y Y Y Y TIME OF DAY 12 00 DATE 01 01 1997 SEA moe melet Press the Enter key to store the value for Date or press the Clear key to re enter the date TIME OF DAY 12 00 DATE 01 01 1997 Press the Setup key to exit the System Setup menu The Setup LED extinguishes O 9 100 214 239 Rev A 6 13 BRANSON Operation System Setup Time of Day The Time of Day parameter affects the Time shown on the display and printed on the Reports 24 hour format only Take this action To get this result PRINTER OFF REMOTE OFF Press the Setup key to display the System Setup menu Its LED lights LANGUAGE ENGLISH TEMPERATURE F 100 TIME OF DAY 12 00 DATE 01 01 1997 Use the Up and Down arrow keys if necessary to display the line showing the Time of Day parameter TIME OF DAY 12 00 DATE 01 01 1997 Press the Line Select key that corresponds to Time of Day 6 14 100 214 239 Rev A Digital Sonifier Entering System Setup Parameters Use the numeric keys to ente
94. ngth This item is sold separately by Branson Refer to the Appendix B Optional Items Parts List for information about specific parts needed for the optional items 4 3 3 Accessories Disruptor horns various horn tips and a variety of chambers or vessels for batch or continu ous processing ate available for use with Branson Digital Sonifiers For a list of Accessories refer to Appendix C Accessories Parts List Microtips Particularly useful for processing small volumes microtips are available in several designs tapered and stepped to meet your application requirements A Coupling Section may be used with a microtip horn for certain applications Microtips Coupling Section and Disruptor Horn Double Step n E Microtip Note Do Not Use with Disruptor Horn Coupling Tapered Microtip Section Disruptor horn 100 214 239 Rev A 4 3 Installation and Setup BRANSON 4 4 CAUTION J DO NOT allow the horn or microtip to contact lab stands beakers test tubes or similar objects Microtip failure may result Breakage of glassware may result in the loss of a specimen Disruptor horns are the base for many microtip applications Disrupter horns are threaded so that they may also be inserted into a Flow Thru chamber attachment see page 4 6 When threaded together a closed chamber is formed between the Tissue Disruptor and the cavity of the Flow Thru Attachment
95. ngth approx 101 147 049 5 at a 1 2 diameter 100 214 239 Rev A B 1 Parts Lists B 2 Optional Items BRANSON Item Description EDP Number 15 pin User I O cable 15 conductor cable 15 ft DB15M to DB15M for User I O port 101 240 014 RS 232 cable Serial cable 25 ft DB9M to DB25F for remote terminal or computer use 101 241 249 Temperature Probe Omega Technologies Model OL 703 PP 1 4 plug connector 200 060 022 WYSE Terminal requires RS 232 cable Wyse WY 55 Terminal with enhanced PC style keyboard 120 230V 50 60 Hz 200 103 228 Okidata 520 printer Okidata Microline 520 printer dot matrix 9 pin parallel interface 120V 50 60 Hz North America 200 143 125 Printer cable Parallel printer cable 15 ft DB25M to Centronics type 100 143 043 Digital Sonifier System Kits The following system Kits may be ordered Each Kit contains the Digital Sonifier system for the voltage indicated 102C converter cordset and horn as noted Item Kit Description EDP Number Kit Digital Sonifier Model Digital Sonifier 250 system kit Pr 250 117V 1 2 Horn 117 volt including 1 2 horn IESU Kit Digital Sonifier Model Digital Sonifier 250 system kit 101 063 589 250 200 245V 1 2 Horn 200 245 volt including 1 2 horn Kit Digital Sonifier Model Digital Sonifier 450 system kit PTT 450 117V 1 2 Horn 117 vo
96. nit is operating When you press the Pause key or type in the command operation of the unit halts and the Pause key s LED is turned on When you press the Pause key or type 1n the H command again the unit resumes operation and the LED is turned off Recall Key R Command The Recall key and the IR command are active only during Parameter Entry mode except when you are changing a parameter value When you press the Recall key or type in the IR command a message is displayed and the LED is turned on Refer to Section 6 6 Save and Recall Presets on page 6 40 for information on how to save and recall presets Save Key ISV Command The Save key and the ISV command are active during Parameter Entry mode except when you changing a parameter value When you press the Save key or type in the SV command message is displayed and the LED is turned on Refer to Section Section 6 6 Save and Recall Pre sets on page 6 40 for information on how to save and recall presets Print Send Key P and S Commands The Print Send key and the P and S commands are only active after a test or experiment has been executed and before another test or experiment begins or the Setup menu parameters are changed For this function to operate a printer a remote terminal or both must be enabled When you press the Print Send key report data is sent to the remote terminal and to the printer Refer to Section 6 7 Printing Sending Reports
97. on Parameters 6 24 Parameter Entry Time 6 26 Parameter Entry Maximum Temperature 6 28 Parameter Entry Pulse On and Pulse Off 6 30 Parameter Entry Pulse Temperature 6 32 65 Remote Computer Terminal 6 34 Enter a parameter using the Remote Terminal 6 34 System Setup Remote Terminal 6 36 System Setup Baud Rate 6 38 6 6 Save and Recall Presets 6 40 6 7 Printing Sending Reports 6 41 6 8 Displays During the Experiment 6 42 Runtime Displays 6 42 Remote Terminal Displays 6 43 6 9 Warning and Error Messages 6 44 100 214 239 Rev A 6 1 Operation BRANSON 6 1 Setup and Operation WARNING J Do NOT touch the vibrating horn or place the vibrating horn against solid objects such as beakers and crucibles Touching or holding the horn can result in burns or injury and contact ing solid materials with the vibrating horn or tip can cause breakage CAUTION J DO NOT run the microtip above an Amplitude setting of 70 Doing so can cause damage to the microtip assembly System Modes You can control the way in which ultrasoni
98. on page 6 41 for a description of the data that is sent to the terminal and printer Enter Key You can press the Enter key on the Digital Sonifier or on the computer keyboard to Cause the system to accept a modified parameter value Clear an error warning message Store a Setup parameter value If you have entered a modified parameter value the system checks the value for validity and format If the value you entered is valid the value is accepted and it replaces the old value The original value 1s lost and cannot be recovered unless it has been stored under a preset ID num ber If the modified value is invalid an appropriate error message 1s displayed 100 214 239 Rev A Digital Sonifier Controls and Commands Clear Key The Clear key on the Digital Sonifier is active at any time when a parameter can be changed If you press the Clear key when you ate modifying a parameter value the entry field becomes blank and the cursor 1s moved to the least significant position If you press the Enter key while the entry field 1s blank no value 1s stored for the parameter Numeric Keys The keys for 1 through 9 and 0 are active at any time when a parameter has been selected to be changed 2 2 2 Remote Terminal Commands This table describes the remote terminal commands and their functions All of the remote ter minal commands must be preceded by an exclamation point Refer to Chapter 6 Operations for a description of the system s
99. onchogenic squamous and benign giant Bone can be decalcified without injury to the cells processed for microscopic sections and diagnosed in a short time Other methods requite extensive treatment time Brain stem and adrenal gland ultrasonic treatment dispersed 10 mg samples 10 ml fluid which is usually difficult without substantial loss of material The suspension was analyzed for nucleotides Brain tissue disintegrates instantly Brevi bacterium 25 ml disrupts in 20 seconds Brevi bacterium acetylicum approximately 3 minutes to disrupt large samples and measure TCA enzyme activity Brine shrimp complete disintegration in 1 minute Brucella abortes separates easily from leucocytes At least 9 antigens extracted Bull sperm contractile protein is easier to extract from tails after sonifying C butyricum C cylinrosporum C kluyveri vegetative cells easily disrupted C pasteurianum 3 minutes disruption for hydrogens reducing Ferredoxin with H2 100 214 239 Rev A Digital Sonifier Appendix A Calcium mouse Ehrlich ascites tumor cells were sonified for 1 minute to determine the amount of bound calcium present Cells were labeled with calcium 45 Candida albicans spores 95 disruption of 1 2 gram dry wt in a 15 ml solution in 35 minutes Carbon black excellent small particle suspension Caryophanon latum glucosamine muramic acid alanine glutamic acid and lysine were obtained Catecholamine can be ext
100. or 0 0 100 0 C Setup If no probe is connected the value will display as TEMP OFF The current Amplitude setting manually set using the Amplitude control on the side of the unit The ampli 1096 minimum to tude affects the amount of power applied during the 100 maximum experiment Max Temp Amplitude This parameter is available for entering the Pulse Tem perature if a temperature probe is connected Entering a value for this parameter puts the system in the Pulse Pause mode The temperature units Fahrenheit or Celsius are determined by the setting of the Tem perature parameter selected during System Setup If a probe is not connected the Pulse Temp value will be displayed as TEMP OFF In Pulse mode the length of time in tenths of seconds that ultrasonics will be On In Pulse mode the length of time in tenths of seconds that ultrasonics will be Off 32 0 F or 0 0 to 3 below Max Temp parameter Pulse Temp Pulse On 0 1 to 59 9 seconds Pulse Off 0 1 to 59 9 seconds 100 214 239 Rev A 6 25 Operation BRANSON Parameter Entry Time The Time parameter if used identifies the time duration of an experiment in hours minutes and seconds dur ing which ultrasonic vibrations are active and are putting energy into your experiment The experiment will stop when the Time entry has elapsed Time may also be used as a Limit CAUTION J Setting the MAX TEMP and TIME parameters to
101. r name 9 List the steps you have already taken 10 What is your application including the materials being processed 11 Have a list of service or spare parts you have on hand tips horns etc 100 214 239 1 5 Safety and Support BRANSON Returning Equipment for Repair Before sending equipment for repair provide as much information with the equip ment to help determine the problem with the system Fill in any details below or on a separate sheet 1 Describe the problem provide as much detail as possible For example is this a new problem Is the problem intermittent How often does it occur How long before it occurs if you are just powering up 2 Is your equipment in an automated system If so is the problem related to Start Stop control or interaction with PLC s or other devices etc 3 If the problem is with an external signal or output which one If known include plug pin e g P29 pin 3 4 What are the setup parameters 5 What is your application e g continuous pulse temperature etc 6 Name and phone number of the person most familiar with the problem 7 Notify the Branson office prior to shipping the equipment NOTE To Return Equipment to Branson you must first obtain RGA number from a Branson representative or the shipment may be delayed or refused 8 For equipment not covered by warranty include a purchase order for the repair costs to avoid delay
102. r the current time based on a 24 hour clock TIME OF DAY 12 00 DATE 01 01 1997 100 SEA mohe melet Press the Enter key to store the value for Time of Day or press the Clear key to re enter the time TIME OF DAY 12 00 DATE 01 01 1997 Press the Setup key to exit the System Setup menu The Setup LED extinguishes 9 o 100 214 239 Rev A 6 15 BRANSON Operation System Setup Temperature Units The Temperature parameter affects whether the system uses Celsius or Fahrenheit units Take this action To get this result PRINTER OFF REMOTE OFF Press the Setup key to display the System Setup menu Its LED lights LANGUAGE ENGLISH TEMPERATURE F 0 100 PRINTER OFF Use the Up and Down arrow keys if REMOTE OFF necessary to display the line showing LANGUAGE ENGLISH the Temperature parameter TEMPERATURE 0 100 PRINTER OFF Press the Line Select key that corresponds REMOTE OFF to Temperature E A TEMPERATURE F 6 16 100 214 239 Rev A Digital Sonifier Entering System Setup Parameters Press the same Line Select key again to toggle between the possible values for Temperature units F or C PRINTER OFF REMOTE OFF LANGUAGE ENGLISH TEMPERATURE F Press the Enter key to store the value for Temperature units PRINTER OFF REMOTE OFF LANGUAGE ENGLISH TEMPERATURE F
103. racted from heart muscle Cellumonas biazotea disruption obtained with retention of malate dehydrogenase activity Chicken spermatozoa completely disrupts in 2 minutes Chlorella completely disrupts in 3 minutes Chloroplasts disrupt in a few seconds Cholesterol apparent permanent suspension in 1 minute in water Desullovibrio vulgaris less than 30 seconds of ultrasonic treatment resulted in release of TCA enzymes Diplococcus completely disrupts in 5 minutes DNA breaks chains on low power instantly Controlled degradation may be obtained Dyes excellent rapid dispersion and homogenization E coli 2 gm wet weight in 10 ml solution completely disrupts in 40 seconds The Sonifer Cell Disruptor has been used extensively in research on this organism Egg whites can be reduced to a homogeneous pipettable solution in 15 seconds on low power Ehrlich ascites disrupts in a few seconds Electron microscopy apertures are quickly cleaned Emulsions 10 ml of most light mixtures become semi permanent emulsions in about 1 minute without emulsifiers Average particle size is usually well under 1 micron Sterile emul sions can be prepared by ultrasonic treatment for feeding to germ free animals Enterococcus excellent disruption Erwina cartovara complete disruption in 1 2 minutes depending on cell concentration Erythrocytes disrupts in a few seconds Euglena gracilis completely disrupts in a few seconds to isolate chloroplas
104. reach Branson Parts Store at the following telephone numbers Branson Part Store direct telephone number 41 22 304 83 58 fax number 41 22 304 83 59 Many parts can be shipped the same day if ordered before 2 30 p m Eastern time A parts list is found in Section 7 of this manual listing descriptions and EDP part numbers If you need replacement parts coordinate the following with your pur chasing agent Purchase order number Ship to information Bill to information Shipping instructions air freight truck etc Any special instructions for example Hold at the airport and call Be sure to give a name and phone number Contact name information 1 7 Safety and Support BRANSON For Your Notes 1 8 100 214 239 Digital Sonifier Overview of Digital Sonifier 2 Introduction to the Digital Sonifier 2 1 Overview of Digital Sonifier 2 1 2 2 Controls Commands 2 3 2 3 System Features 2 9 2 1 Overview of Digital Sonifier The 250 450 Digital Sonifier is often used by laboratory personnel in the medical and chemical process fields T he system consists of three core elements the power supply the controls and the converter and horn The system can interface with customer equipment including a remote computer or terminal dot matrix printer temperature probe and User I O Figure 2 1 Digital Sonifier
105. rect value Refer to Table 6 1 System Setup Parameters on page 6 9 and Table 6 2 Operation Parameters on page 6 26 for the limits for specific parameters Are you sure you want to overwrite You tried to save a set of parameter values under a preset ID that is already in use To overwrite the existing values in the Pre set ID press Line Selector key 3 To enter a different preset ID press Line Selector key 4 The previous screen is displayed for you to enter a different pre set ID Printer error You issued a print com mand but there was no response from the printer The system was turned on with the Printer On but with no printer con nected Check that the printer is correctly con nected and turned on Check the Model parameter to see that the correct printer model has been set Check that the Printer parameter is set to On THE DATA FORMAT ENTERED FOR parameter S INCORRECT PLEASE ENTER AGAIN The parameter value that you entered was in the wrong format Enter the value in the correct format Refer to Table 6 1 System Setup Parameters on page 6 9 and Table 6 2 Operation Parameters on page 6 26 for valid param eter formats INVALID COMMAND RECEIVED PLEASE ENTER AGAIN You entered an invalid command through the remote computer or ter minal Enter a valid command Refer to Table 6 2 Operation Parameters on page 6 26 for valid remote
106. require tuning Tuning is done in the factory and cannot be per formed by the operator 7 2 Reconditioning the Stack Interface Ultrasonic system components work most efficiently when the mating surfaces of the Con verter and Horn combination also called a Stack are flat in solid contact and free from fretting corrosion Fretting corrosion refers to black crusty build up resulting from friction between metal parts that appears on the Stack mating surfaces Poor contact between mating sutfaces wastes power output makes tuning difficult increases noise and heat and may cause damage to the converter 100 214 239 Rev A Digital Sonifier Reconditioning the Stack Interface 7 2 1 Refacing the Mating Surfaces NOTE 1 Never clean the Converter or Horn mating surfaces with a buffing wheel 1 Disassemble the Converter Horn Stack and wipe the mating surfaces with a clean cloth or paper towel 2 Examine all mating surfaces If any mating surface shows corrosion or a hard dark deposit recondition it 3 If necessary remove the threaded stud from the part 4 Tape a clean sheet of 400 or finer grit emery cloth to a clean smooth flat sur face such as a sheet of plate glass NOTE 1 Be careful to avoid tilting the part and losing flatness of the surface Doing so may make the system inoperative due to improper mating surfaces 100 214 239 Rev A 7 3 Maintenance BRANSON Lapping Procedure 5 Holding the p
107. response to each of these commands and the actions you should take at each response Table 2 1 Remote Terminal Commands When you want to IT Enter the TIME duration value IMT Enter the MAXIMUM TEMPERATURE value IPO Enter the PULSE ON time value IPOF Enter the PULSE OFF time value IPPT Enter the Pulse Temperature value IS Send all report data to the remote terminal IP Print all report data if a printer is enabled IR Recall a particular preset IH Pause operation of the Digital Sonifier until you send another H command IMT Enter the Maximum Temperature value ITS Test the system for two seconds ION Start the experiment IOFF Stop the experiment Lock out the keypad on the front panel The keypad is enabled IKL again if you send another KL command or if you turn the power off on the Digital Sonifier and then turn it on ISV Save the parameters entered IRP View all the test or experiment parameter values 100 214 239 Rev A 2 7 Introduction to the Digital Sonifier BRANSON 2 8 2 2 3 Back Panel Connections Figure 2 3 Back Panel of Digital Sonifier Fan IEC type power cordset connector Fuse holder AS d Converter cable Cz X Temperature Probe connector User I O connector Printer Terminal Parallel EN RS 232 The back panel
108. ring treatment The Flow Thru Rosett Cell 15 not suited for difficult cells 100 214 239 Rev A 4 7 Installation and Setup BRANSON 4 8 Soundproof Enclosure Although ultrasound is above the normal range of human hearing audible sound sometimes occuts when liquids are treated ultrasonically especially due to cavitation produced by ultra sonic vibration The Soundproof Enclosure can be used to reduce this to an acceptable level It is especially useful when the Sonifier 15 used for extended periods of time The Soundproof Enclosure 15 also useful at minimizing splashing while the experiment is run ning Cooling within the enclosure may be required for certain applications Detail of the enclo sure may vary from those depicted below Converter cable Converter Accessory ae HHH enclosure Horn Digital Sonifier Tip 2 IL 000 000 88 000 005 el Ring stand support Enclosure door 100 214 239 Rev A Digital Sonifier Assembling the Equipment 4 4 Assembling the Equipment The Digital Sonifier unit is pre assembled and requires no special tools however other com ponents must be connected to the unit in order for the system to operate Some assembly of the ultrasonic horn 1s required as described 1n the following sections 4 4 1 Setup Procedure To set up your Digital Sonifier for an experiment take the follo
109. rom silt and clay fractions Serial number restoration used in crime laboratories to restore obliterated serial numbers Serratia marcescens complete breakdown of a concentrated solution in 1 minute Serum quickly homogenized Serum cholinesterase activated by ultrasonic treatment Different cholinesterase isoenzymes may be activated and inactivated selectively Shale excellent disaggregation of all fine grained sedimentary rocks Shellfish by drilling a clean hole with the micro tip various fluids or samples may be withdrawn or injected from living shellfish without destroying the animals Shigella quick disruption 100 214 239 Rev A Digital Sonifier Appendix A Skin 1 gm disintegrates 11 about 4 minutes Epidermal homogenates that respire and utilize substrate can be extracted Soil separates solid particles without use of oxidants acids or peptizing agents and yields stable suspensions Sperm human tails are broken instantly Heads are broken in 20 minutes Sputum cancer cells are more easily detected after ultrasonic treatment due to even dispersion of cells and sputum and complete liberation of the cells from sputum Staphylococcus a concentrated solution disrupts 98 in 10 minutes With 1 gm cells wet wt to 2 gm water 54 5 mg ml of protein was released Starch obtained by extracting from green plant leaf homogenate Streptococcus Group A a 20 suspension in a 15 ml solution completely disrupts in 15
110. rrect system settings for the horns you are using Suggested Amplitude Values for various Horns Ts Amplitude Control Settings Horn EDP No Description n 50 1004 101 147 087 Stepped Disruptor 0 0008 0 0029 0 0057 101 147 042 Catenoidal Disruptor 0 0008 0 0029 0 0087 101 147 041 Esponential Disruptor 00004 0 0013 0 0026 19 457 029 Euros iM PM ond JOEY m qM All measurements in microns unless otherwise specified Approximate Microtip Amplitudes 2 Amplitude Control Settings Horn EDP No Description iQ 4096 xe 101 148 062 1 8 Diameter Tapered AR 101 148 069 3 16 Diameter Tapered eos s Su 101 148 070 1 4 Diameter Tapered eee nut X 101 063 212 Double Step m sores All measurements in microns unless otherwise specified 4 12 100 214 239 Rev A Digital Sonifier Input Power Requirements 4 5 Input Power Requirements The input power requirements for the Digital Sonifier are 117 VAC 50 60 Hz North American model 200 245 VAC 50 60 Hz Export models only The Digital Sonifier 15 equipped with an IEC type power cord connector The unit requires a single phase three wite 50 60 Hz power source WARNING To prevent the possibility of electrical shock always plug the Digital Sonifier unit into a grounded power source The system is fuse protected
111. rt Stop button or sending a ON com mand will Start your setup Ultrasonic power is delivered to the horn whether continuous or pulsed and the front panel display will show the runtime information including the percentage of power used on its bottom line as a bargraph display and the LED next to the Start Stop button remains lit while the appli cation is running If you set Limits your application will Stop automatically upon reaching those limits Test From the Ready state the system enters the Test state when you press the Test key or issue a TS command to enter the Test state causing ultrasonics to be sent to the horn After two seconds the sys tem returns to the Ready state Print If you have a printer connected and after running a cycle or testing the setup you may print a report of your experiment Printing is started by pressing the Print Send key or sending a P command After printing the system returns to the Ready state Report If you are using the Remote Terminal option and after running a cycle or testing the setup you may send a report of your experiment from the system to your terminal After the report is sent the sys tem returns to the Ready state Hold Pause When you press the Hold Pause key or issue an H command during an experiment or test the system stops operating stops all timer functions and enters the Hold Pause state To resume oper ation press the Hold Pause key again or issue another H comma
112. sday August 23 2001 10 52 AM Digital Sonifier 100 214 239 parameter entry amplit de neisitte 6 29 maximum temperature 6 27 pulse 6 33 DUIS ON E 6 31 pulse temperature 6 35 UMNE 6 25 parameters entering from 6 23 pause eR dedo ate aoa 6 37 performance 5 2 physical description 5 3 power output 105 7 2 power requirements E E TE 4 10 precautions inen 1 1 presets save and recall essen 6 36 preventing cross contamination A 3 printer 6 5 printer 4 11 printing and sending 6 39 pulse off parameter entry 6 33 pulse on parameter entry 6 31 pulse temperature parameter eHly ecrit etude 6 35 CONFIDENTIAL For Review Only Index 1 5 2nd Review copyfilename C Documents and Settings St phanie My Documents CD ROM Sonifier Manuals Digital Englishi
113. sonified 1 2 minutes and the supernatent was used for enzyme experiments without further treatment Extracts could be frozen and stored without loss of activity Thiouric acid dissolved in a few seconds Thymus cells complete disruption in 15 seconds Tissue culture cells complete disruption in a few seconds To avoid damage to free organelles and to obtain intact lysosomes use low power at short exposute Toxin and antitoxin one example of many Toxin preparations of whole cell lysate WCL of the Inaba serotype strain 569E of the classic biotype of cholera vibtio were grown on 3 Bacto peptane agar and harvested in distilled water at 18 hours The unwashed suspensions were sol ubilized ultrasonically clarified by centrifugation and the supernate freeze dried for the titra tion of cholare toxin the rabbit ileal loop Toxoplasma gondii can be separated form white blood cells without injury Transplantation antigens were extracted from spleen thymus and lymph nodes Trichomonas foetus complete disruption in a few seconds Triolein complete stable emulsion in 2 minutes Trypanosomes concentrated 10 ml solution completely disrupts in 4 minutes Uterus muscle a 1 5 gm 3 cc solution completely disrupts in 3 minutes for coenzyme Q determination Vaccines numerous advantages such as more antigenic material released than usual and the production of vaccines not obtainable by classified methods Various bacilli complete d
114. stomer provided Remote Computer or Terminal Take this action To get this result PRINTER OFF REMOTE OFF Press the Setup key to display the System Setup menu Its LED lights LANGUAGE ENGLISH TEMPERATURE F 100 PRINTER OFF Use the Up and Down arrow keys if neces REMOTE OFF sary to display the line showing the LANGUAGE ENGLISH Remote parameter TEMPERATURE 100 PRINTER OFF Press the Line Select key that corresponds PEMOTES YEE to Remote LANGUAGE ENGLISH TEMPERATURE F 100 6 36 100 214 239 Rev A Digital Sonifier Remote Computer or Terminal Press the same Line Select key to toggle between the two possible values for Remote On if a remote computer or termi nal will be enabled Off if it will be disabled PRINTER OFF REMOTE OFF LANGUAGE ENGLISH TEMPERATURE F 100 Press the Enter key to store the value for Remote PRINTER OFF REMOTE OFF LANGUAGE ENGLISH TEMPERATURE F 100 Press the Setup key to exit the System Setup menu The Setup LED extinguishes 100 214 239 Rev A 6 37 Operation BRANSON System Setup Baud Rate The Baud Rate parameter identifies the communication rate of the Serial Port used for the remote computer or terminal NOTE 1 The Baud Rate parameter configures the data communications rate for the optional remote computer or terminal The Data Bits format and
115. t release of acetokinase Lenconostoc mesenteroides ultrasonic treatment for 15 minutes using high power for dis ruption Leukocyte lysozyme activity in myelocytic leukemia the cell suspension was ultrasonically treated and samples assayed for lysozyme activity The lysozyme concentration of the leuko cytes ug 1 0 cells was determined Linoleic acid made suspension in water in 30 seconds Liver tissue 1 gm homogenizes in less than 1 minute Lung tissue 1 gm homogenizes in 2 minutes Lymphacytis complete disruption in 15 seconds Lymphocyte nuclei complete disruption in 6 minutes Lymph gland direct injection lymphography with a modified radiopaque emulsion was obtained by ultrasonic treatment in a functional procedure producing lymphatic structure detail Lysossomes released enzymes quickly Malaria prolozoa fast excellent disruption Maple bark spores complete disruption in 14 minutes Measles disruption of virus antigen clumps present in infected cells on low power Ultrasonic treatment increased antigen titer 4 8 fold Methanobacillus omelianskii 1 gm cells wet wt ml completely disintegrates in 2 minutes for assaying methane Microbacterium lacticum ultrasonic treatment used for malate dehydrogenase extraction Micrococci a 13 ml solution completely disrupts 15 minutes 100 214 239 Rev A A 9 Application Information BRANSON A 10 Micrococcus lactiliticus 75 ml of a 20 suspension was disintegrat
116. terminal commands THE SYSTEM COULD NOT INTERPRET THE LAST TRANSMISSION PLEASE TRY SENDING AGAIN The system could not interpret the data that was sent from the remote computer or terminal Send the data again 6 44 100 214 239 Rev A Digital Sonifier Maintenance and Troubleshooting 7 Maintenance 7 1 Maintenance and Troubleshooting 7 1 7 2 Reconditioning the Stack Interface 7 2 7 3 Troubleshooting Charts 7 5 7 4 Interconnect Diagram 7 9 7 1 Maintenance and Troubleshooting The Digital Sonifier is a self contained system that requires no internal servicing except fora protective fuse and there are no user serviceable parts inside the unit The ultrasonic tooling horns and tips may require periodic inspection and maintenance to ensure optimum perfor mance The tooling components are subject to wear and may require replacement after a period of time depending on the applications If you have a problem operating your unit refer to Table 1 System Trouble Analysis Chart on page 7 7 to locate the symptom that most clearly describes your problem Tip Erosion Horn tips can erode Tip erosion is a side effect of the cavitation process that occurs when liq uids are exposed to ultrasonic energy The rate of erosion depends on the intensity of power applied the corrosiveness of the liquid being treated
117. tes Propionobacteria releases sulfhydro groups intact 70 ml of 20 suspension processed for 10 minutes Propionibacterium shermanii 2 minutes for extraction of citrate synthose Proteus excellent disruption Pseudomonas aeruginosa rapid complete disintegration Pseudomonas fluorescens 2 gm wet wt in 10 ml completely disrupts in 1 minute Pulmonary cytodiagnosis the mucous in sputum can be evenly dispersed giving a quick representative sample of cells for cytologic examination Cells are liberated from the mucous of sputum that had been immersed in 50 alcohol or a fixative Ragweed pollen 15 ml dispersion completely disintegrates in 11 minutes Rat bone 1 2 gm disintegrates in 4 minutes Rat liver complete disruption 3 minutes Rat liver mitochondria ultrasonic treatment has been used extensively for the varied research performed on this material Disruption occurs in seconds Rat skin 1 gm completely disintegrates in 4 minutes Red and white blood cells ultrasonic treatment breaks particle size to 100 Angstroms Com plete disruption in 1 minute 25 gms 100 ml saline or plasma sample treated 15 seconds 35 disruption Adenosine triphosphate was shown to be membrane bound by this method Reovirus dissociates cell bound and aggregated virus Maximum titer with 4 ml of virus was achieved in 2 minutes 100 214 239 Rev A A 11 Application Information BRANSON A 12 Retinal outer segments ultrasonic treatm
118. tions should be observed when operating the Digital Sonifier Make sure that the equipment is properly grounded DO NOT operate if it is not The unit is equipped with a three conductor cord and three prong grounding type plug and must be plugged into a three prong grounding type wall receptacle DO NOT under any circumstances remove the power cord ground prong DO NOT operate the equipment with the cover removed High voltage is present within the equipment when connected to plant wiring DO NOT turn on the ultrasonics without the converter and horn attached DO NOT touch the horn or tip when ultrasonics are active When handling removing or attaching a horn or tip be sure that the ON OFF switch is set to OFF Touching the horn or tip while the unit is on can result in serious personal injury DO NOT allow the horn or microtip to contact lab stands beakers or similar objects Horn microtip failure may result DO NOT operate the equipment at more than 70 amplitude when using a microtip horn Establish a standard of operation and test equipment periodically 1 1 Safety and Support BRANSON 1 2 General Precautions 1 2 1 Intended Use of the System The 250 450 Digital Sonifiers can be used to disrupt cells bacteria spores or tis sue and are ideal for initiating and accelerating chemical biochemical and phys ical reactions and for de gassing liquids With the Digital Sonifier you can prepare an emulsion to 0 01 mi
119. ts 6 minutes Haemophilus pertussis preparation of successful immunological complexes Heart muscle 1 gm disintegrates in 6 minutes HeLa cells disruption to free virus in a few seconds without injury Hemophilus pertussis an immunological compound prepared Herpes virus may be quickly released without injury Histoplasma capsulatum ultrasonic treatment for 7 minutes completely ruptures cells prepared by formalin fixation Good enzyme activity 1s obtained Human serum proteins ultrasonic treatment causes a reproducible change in the electro phoretic behavior of normal human serum consisting of an increase in material migrating 1n the x and b globulin zones with a reduction in the albumin and y globulin fractions Hydrocortisone smaller crystals were produced by ultrasonic treatment A 8 100 214 239 Rev A Digital Sonifier Appendix A Hydrophilic vegetable gums disperses and solubilizes hydrophilic vegetable gums in water makes dispersions of added particulate matter Intracellular membrane disruption and particle size reduction obtained 1n 30 60 seconds Isoenzymes selectively activated with respect to time and intensity of treatment Kidney 1 gm disintegrates in 3 minutes Kidney stones easily broken 1n seconds in vitro Klebsiella excellent disruption L arabinosis complete disruption to free virus in 2 minutes without injury Lactobacillus 0 5 gm in 15 ml completely disrupts 11 minutes Excellen
120. ts 100 214 239 Rev A A 7 Application Information BRANSON Eugoena complete disruption in 12 minutes 90 disruption in 8 minutes with pigment released Fat extraction fat can be emulsified without injuring tissue with proper power selection Lipid layer can be stripped from spores and mycobacteria Fibrin complete suspension of 0 125 gm in 30 minutes Fish gill complete disruption of 20 mg in 30 seconds Fish tissue tissue homogenization for extractions and excellent particle size reduction in 8 minutes per 10 gm Fluorocarbons extended treatment time will break down particle size to well under 1 micron and gives a fine homogenate Fossils low power will clean debris from delicate fossils without injury Micro fossils such as pollen can be separated from rocks to help identify the geological age of the strata Removal of rock matrix Gamma globulin the Sonifer Cell Disruptor was used to solubilize protein as one of the steps in the biosynthesis of gamma globulin from rabbit spleen Gangliosides immunochemical and structure studies used ultrasonic treatment as one step Gastric mucosa placing scrapings into a test tube and test tube into new water filled cup horn caps permits these cells to be separated without breakage Graphite molybdenum disulfide an excellent dispersion of this lubricant was made in a silicate binder Guanine produces colloidal suspension in 1 minute Gymnodinium solution completely disrup
121. us veg cells disruption in a few seconds B cereus spores disruption of 10 mg 6 ml in 13 minutes B megaterium spores complete breakage of a concentrated 6 ml solution in 15 minutes B sphaericus major disruption in 1 3 minutes 100 214 239 Rev A A 5 Application Information BRANSON A 6 B stereothermophilis spores complete disruption in 2 minutes B subtilis disruption of 5 gm wet wt 15 ml buffer in 5 minutes B subtilis veg cells heavy suspension clears 1 minute Bacillus stereothermophulus thermophillic spore form 98 disruption of 70 ml of 40 suspension in 15 minutes Bacillus brevis 1 15 W V in 3 minutes Bacteroides Symbiosis 1 phosphorfructokinase a soluble enzyme has been isolated from this anaerobe by ultrasonic treatment A 25 ml suspension was sonified for 10 minutes and centrifuged at 36 000 xg for 10 minutes Baker s yeast saccharomyces cerevisiae see Yeast Blastomyces dermatitidis 95 disruption in 3 minutes Blood cells ted and white cells can be disrupted in a few seconds Boll weevil tissue complete homogenization 1n a few seconds Bone compact bone can be sonified and processed for microscopic sections in minutes Other methods can require up to a week Bone specimens treated 1n this way yielded large numbers of intact cells with little distortion Malignant criteria are easily recognized Tumor types studied were osteosarcoma chondrosarcoma liposarcoma chordoma metastic br
122. w r 250 up to 200 watts 450 up to 400 watts Temperature Operating 41 F 5 C to 104 F 40 C ambient Storage and shipping 20 F 29 C to 158 F 70 C Factory set no tuning necessary No user accessible adjust Tuning ments Amplitude control at maximum setting System settings are covered in Chapter 6 100 214 239 Rev A 5 1 Technical Specifications 5 2 BRANSON 5 2 Physical Description The following table outlines the physical characteristics of the Digital Sonifier Converter Weight 4 lbs 1 8 kg with horn Converter Length 7 inches 180 mm Converter Diameter 2 1 2 inches 60 mm Horn Tip Diameter 1 8 inch 1 0 inch 3 2 25 4 mm depending on process needs and Accessory Tip selected Power Supply Weight 17 lbs 7 7 kg Temperature Probe Omega Technologies model OL 703 PP It is 0 125 inches in diameter and 4 5 inches long 100 214 239 Rev A Digital Sonifier System Performance Benchmark 5 3 System Performance Benchmark Each application and system configuration 1s slightly different System performance will vary when you change setup parameters and if your horn or tips change and can affect the results of yout experiments Creating a benchmark of your initial setup and performance can be useful at a later date in identifying a change in performance and can also help in recreating your exact initial setup
123. wing steps Step Action 1 Connect the tip horn and converter following the procedure in Section 4 4 2 Connecting Tips Horns and Converters on page 4 10 2 Mount the converter horn assembly in a laboratory stand or other suitable support Secure the clamp on the converter housing 3 Set the ON OFF switch on the front of the unit to the O OFF position Plug the line cord into the unit and then into an appropriate electrical 4 power outlet ensuring that the power supply is grounded to prevent elec trical shock remove a horn use spanner wrenches shipped with the system Never attempt to remove a horn by holding the converter housing 1n a vise If necessaty secure the largest portion of the horn in a soft Jawed vise See Section 4 4 2 Connecting Tips Horns and Converters on page 4 10 100 214 239 Rev A 4 9 Installation and Setup BRANSON 4 10 4 4 2 Connecting Tips Horns and Converters Connecting the Horn to the Converter To connect the horn to the converter take the following steps Step Action 1 Clean the contacting surfaces of the converter and horn and remove any foreign matter from the threaded stud and threaded hole Coat one of the mating surfaces completely with a very thin film of silicone 2 grease without additives using an amount about the size of a paper match head Excessive silicone grease can diminish performance 3 Thread the horn stud into
124. with a replaceable glass fuse 5x20mm slow blow type refer to the data tag on the system This fuse should never blow under normal operating conditions The fuse holder is found on the rear of the unit as part of the IEC power connector 4 6 Electrical Connections to Equipment All of the connections to the Digital Sonifier are made to the rear of the unit using industry standard connectors Refer to Section 2 2 3 Back Panel Connections on page 2 8 for connector locations See Appendix B and C for accessory part numbers 4 6 1 Power Cord North American units are shipped with a 3 conductor 117 Volt cordset NEMA 5 15P to IEC jack It connects to an IEC type connector on the rear of the unit The plug end connects to your main voltage receptacle which should be properly fused depending on your site require ments It requires a conventional NEMA 5 15R receptacle for installation Export units are shipped with a standard Harmonized European cordset having an IEC type jack CAUTION J If your cordset does not match your main power receptacle verify that you have the correct voltage available Do not connect the system if the voltage rating of the unit is incorrect for your location as this can damage the unit 100 214 239 Rev A 4 13 Installation and Setup BRANSON 4 6 2 Remote Terminal Connection The remote terminal is connected to the rear of the Digital Sonifier through an industry typical RS 232 connection See Back Panel
125. your experiment 6 2 3 Remote Terminal An optional Remote Terminal device may be used to setup and to run the experiment The remote device can fully control the Digital Sonifier using the built in serial interface Instructions for using the remote computer or terminal and the Remote Terminal commands that you can use to enter parameters from your remote com puter or remote terminal are described in Section 6 5 Remote Computer or Terminal beginning on page 6 35 NOTE 1 Remote Terminal commands apply to a Remote Computer or a Remote Terminal The Setup commands only indicate Remote Terminal for simplicity 6 2 4 Amplitude Control The Amplitude control is found on the right side of the system chassis it is a large circular control protruding through the side of the Front Panel It rotates up and down to regulate the Amplitude value from minimum to maximum The Amplitude value 15 displayed on the Front Panel display The Amplitude is displayed numerically on the bottom line of the Ready display in 1 units to a system max imum of 100 and after an experiment has been run Amplitude may be adjusted while an experiment 1s run ning An example of the Amplitude numerical display is shown in Figure 6 1 on page 6 7 When the system 1s running the Amplitude of the ultrasonic energy is displayed on the bottom line of the first page of the runtime display as a bargraph 5 increments An example of the bargraph display 1s shown

Digital Sonifier® Models 250 & 450 User's Manual

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Digital Sonifier&reg; Models 250 &amp; 450 User's Manual

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Digital Sonifier® Models 250 & 450 User's Manual