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Lambda 25, 35, 45 User's Guide

1. In the event of damage or missing parts file an immediate claim with the authorized carrier and inform your PerkinElmer office or representative NOTE PerkinElmer offers an installation service for your spectrometer Contact your local PerkinElmer office or representative for further information 58 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Installation Connecting to the Electrical Supply Electrical Hazard To prevent potential injury to yourself and damage to the instrument first make the electrical connections between the instruments in the system before connecting to the line power supply WARNING The spectrometer automatically adjusts to the correct operating voltage Before starting the instrument for the first time make sure that the correct fuse is fitted to your line power supply If you have a different fuse change it for the correct one Do not connect the spectrometer to the line power supply if the wrong fuse is fitted 1 Make sure that the correct fuses are fitted in the holder at the rear of the spectrometer see Changing Fuses on page 102 100 V 120 V 6 3 A slow blow 210 V 240 V 3 15 A slow blow NOTE The module has two fuses 2 Make sure that the plug fitted to the line power cord provided with the spectrometer is suitable for your local electrical outlets 3 After all connections have been made between the various compo
2. Responsible body individual or group responsible for the use and maintenance of equipment and for ensuring that operators are adequately trained Operator person operating equipment for its intended purpose Notes cautions and warnings Three terms in the following standard formats are also used to highlight special circumstances and warnings A note indicates additional significant information that is provided with some procedures Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Introduction CAUTION We use the term CAUTION to inform you about situations that could result in serious damage to the instrument or other equipment Details about these circumstances are in a box like this one o 8 O o 6 Caution Achtung Bedeutet daf die genannte Anleitung genau befolgt werden muf um einen Geriiteschaden zu vermeiden Caution Bem rk Dette betyder at den nevnte vejledning skal overholdes naje for at undg en beskadigelse af apparatet Caution Advertencia Utilizamos el t rmino CAUTION ADVERTENCIA para advertir sobre situaciones que pueden provocar aver as graves en este equipo o en otros En recuadros ste se proporciona informaci n sobre este tipo de circunstancias Caution Attention Nous utilisons le terme CAUTION ATTENTION pour signaler les situations susceptibles de provoquer de graves d t riorations de l instrument ou d aut
3. Insert the matching cell with the same low absorbing solvent into the reference cell holder The first cell remains in the sample cell holder Repeat steps 5 to 9 with the reference cell holder but this time obtain the lowest possible transmittance reading on the display This completes the fine alignment procedure NOTE When the cell holder has been aligned once you can take it out and reinstall it without aligning it again Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Installation Minimum Volume Applications To measure minimum sample volumes use microcells offered by PerkinElmer The minimum sample volume required is a function of the cell internal width or volume and is specified below Cell Type Cell Pathlength Minimum Part Internal Volume Number Width Required Height of 2mm 1 200 uL B0631071 liquid slightly pair 1 Cell Pathlength Minimum Part Volume Volume Number Required completely liquid uL 5 uL 10 uL B0631081 30 uL 50 uL B063 1079 NOTE You should align microcells very carefully in the radiation beam by following the procedures in Aligning the Single Cell Holder on page 65 When aligning microcells fill each cell with the minimum volume of liquid specified in the above table to make sure that the liquid meniscus is not in the radiation beam more than height of 4mm cm 400 uL B063 1064 beam pair 69 Artisan Scientific Quality
4. Right side view K t_ 56 cm Figure 7 Space Requirements 56 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Installation Equipment Provided Spectrometer Lambda 25 Spectrometer Double beam OR Lambda 35 Spectrometer Double beam with four selectable slit widths OR Lambda 45 Spectrometer Double beam with four selectable slit widths with pre monochromator according to order Components The components listed below are provided with the spectrometer Quantity Part Number Line power cord with suitable connector varies for electrical outlets Lambda 25 35 45 manuals kit 09934419 9 pin RS232 cable 09410022 ER rot ee Ee reem o CCOO CT Ee ES Es UV WinLab software kit L6100013 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com 57 Lambda 25 35 45 User s Guide Unpacking your Instrument Unpacking and Inspection 1 Unpack the components carefully Keep the packing materials for possible future storage or reshipment 2 Examine the components for any signs of damage in shipment e Check the entire outer casing of the spectrometer for damage e Make sure that terminals fuse holders etc are not damaged e Open and close the sample compartment cover checking that it moves freely without binding e The compartment must be free of dust or other foreign matter
5. Startup 1 Open the sample compartment cover 2 Make sure that the beam paths are free that is e No objects for example cables project into the beam paths e No samples are in the sample compartment e Accessories are properly installed NOTE Jr the sample compartment is obstructed during the startup procedure the spectrometer will not initialize correctly 3 Close the sample compartment cover 4 Switch on the power switch 5 Wait for approximately 3 minutes until all initialization is complete 6 Switch on the accessories 7 Ensure the PC is correctly connected 8 Start UV WinLab 61 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 62 Shutdown 1 Close down UV WinLab 2 Switch off the accessories 3 Open the sample compartment cover 4 Remove samples and cells from the sample compartment 5 If accessories for example flowcell are installed in the sample compartment clean them thoroughly 6 Close the sample compartment cover 7 Switch off the spectrometer Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Installation Installing the Single Cell Holder Description Locking screw for horizontal alignment Vertical adjustment screw Milled post Lifter LAMBDA Locking screw for horizontal alignment 9 Figure 9 Sing
6. 1 Open the lamp compartment 2 Remove the lamp baffle by slackening the thumbscrews and lifting the lamp baffle vertically upward 99 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Thumbscrew Thumbscrew Figure 24 Lamp baffle 3 Loosen the thumbscrew holding the attenuator in place Deuterium lamp S J Ma assembly a amp energy attenuator Figure 25 Deuterium lamp assembly 100 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Maintenance 4 Slide the attenuator downwards into the beam 5 Tighten the thumbscrew Follow the above procedure in reverse to slide the attenuator out of the beam 6 Replace the lamp baffle using the reverse of the procedure described in step 2 7 Close the lamp compartment Lamp Alignment Procedure Due to the prealigned mounts the alignment of lamps after installation is generally so good that further alignment is not required 101 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Changing Fuses Electrical hazard To prevent potential injury to yourself and damage to the instrument switch OFF the instrument and disconnect it from the line power supply before you change the fuses WARNING The fuses are located in a
7. replacement UV Radiation The lamps emit intense radiation which can damage your eyes Do not open the lamp compartment when the lamps are on Do not gaze into a lighted lamp 93 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 1 Switch off the spectrometer and unplug the line power cord 2 Remove the lamp compartment cover by pressing down the catch and pushing the cover to the left 3 Remove the lamp baffle by slackening the thumbscrew for the deuterium lamp and lifting the lamp baffle vertically upward Thumbscrew Thumbscrew Figure 19 Lamp baffle 4 Carefully pull the white ceramic connector from the rear of the halogen lamp 94 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Maintenance White ceramic connector Thumbscrew Halogen lamp assembly Figure 20 White ceramic connector 5 Remove the lamp assembly from the bracket by slackening the thumbscrew and pulling the lamp mount vertically upward Save the thumbscrew for use with the new lamp assembly 6 Unpack the new lamp assembly taking care to hold it only by the metal mount to prevent fingermarks on the bulb 7 Slip the slot at the base of the lamp mount over the stud on the bracket in the lamp compartment and then secure with the thumbscrew 8 Carefully push the ceramic connecto
8. Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 70 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Accessories 5 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com 1 Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Accessories Accessory Installation To operate the spectrometer with some accessories for example the Peltier Temperature sensor an accessory panel kit L6000500 is required Procedures for installing the accessories in the spectrometer are described in the directions provided with the respective accessory To install certain accessories you need to remove the sample compartment cover The required procedures are described below In the directions provided with some sample handling accessories reference is made to earlier models in the Lambda Series of spectrometers These directions are generally applicable to the current series of instruments since the sample compartment is standardized 73 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Accessory Connector Panel To facilitate manufacturing procedures a common connector panel is used for the Lambda Series of instruments TEMPERATUR
9. charges meme si l instrument a t d connect de toutes les sources de tension Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Introduction W02 02 A Lethal voltages are present in the instrument e Even with the power switch OFF line power voltages can still be present within the instrument WARNING e When the instrument is connected to line power terminals may be live and opening covers or removing parts except those to which access can be gained by hana is likely to expose live parts e Capacitors inside the instrument may still be charged even if the instrument has been disconnected from all voltage sources Nello strumento sono presenti tensioni mortali j CD e Anche se l interruttore di alimentazione in posizione OFF la tensione di linea pu essere ancora presente all interno dello strumento e Quando lo strumento collegato alla rete di alimentazione i terminali possono essere sotto tensione aprendo le calotte di protezione o rimuovendo alcune parti ad eccezione di quelle raggiungibili con la mano e possibile esporre altre parti sotto tensione e Anche se lo strumento stato scollegato da tutte le fonti di tensione i condensatori al suo interno possono essere ancora carichi Gevaarlijke spanningen in het instrument gevaar van letsel NL e Ook in uitgeschakelde toestand kan wanneer het instrument op de netvoeding is aanges
10. make any new electrical connections WARNING Installation Overview Connector on Spectrometer Sample Compartment Cover TEMPERATURE J11 J10 SPHERE J88 goto B0167808 Figure 14 Multisipper electrical connection 80 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Accessories Autosampler Electrical hazard To prevent potential injury to yourself and damage to the instrument switch OFF all instruments in the system and disconnect them from the line power supply before you alter or make any new electrical connections WARNING Installation Overview Spectrometer PELTIER 430 J11 440 o 00 J14 AS 93plus Autosampler External P S U Figure 15 Autosampler electrical connection 81 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Operating with Accessories General Accessories are components or instruments that are installed or connected in the sample compartment or otherwise connected to the spectrometer For some of these accessories parameters have to be taken into account in the UV WinLab methods When the accessories described below are connected to the instrument they will be detected automatically and the appropriate windo
11. provide an efficient laboratory ventilation system to remove vapors that may be produced when you are performing analyses 15 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Waste Disposal Waste containers may contain corrosive or organic solutions and small amounts of the substances that were analyzed If these materials are toxic you may have to treat the collected effluent as hazardous waste Refer to your local safety regulations for proper disposal procedures Deuterium lamps and other spectral lamps are maintained under reduced pressure When you dispose of lamps that are defective or otherwise unusable handle them correctly to minimize the implosion risk UV Radiation You should be aware of the health hazards presented by ultraviolet radiation e When the deuterium UV lamp is illuminated do not open the spectrophotometer covers unless specifically instructed to do so in the manual e Always wear UV absorbing eye protection when the deuterium lamp is exposed e Never gaze into the deuterium lamp 16 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Warnings and Safety Information Lifting the Lambda 25 35 45 Consult local codes or practice issued by safety advisors before attempting to lift the instrument WARNING As the Lambda 25 35 45 weighs approximate
12. rede antes de estabelecer ou modificar qualquer liga o el ctrica 0 000 32 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Introduction 1 WO03 02 UV Radiation Risk of Eye Damage j The lamp emits intense UV radiation which can damage your eyes Do not gaze into a lighted lamp Always wear UV absorbing safety glasses when looking at the radiation from the lamp 5 UV Strahlung Gefahrdung der Augen Die Strahlungsquelle Lampe emittiert intensive UV Strahlung und kann dadurch Augensch den verursachen Tragen Sie eine Schutzbrille die die emittierte UV Strahlung ausreichend absorbiert wenn Sie in die leuchtende Strahlungsquelle Lampe schauen Ultraviolet str ling farligt for jnene Str lingskilden lampen emitterer ultraviolet str ling og kan derfor for rsage ajenskader B r beskyttelsesbriller som absorberer den emitterede ultraviolette str ling tilstreekkeligt nar De ser ind i den lysende stralingskilde Radiaci n de rayos ultravioleta La l mpara emite una intensa radiaci n de rayos ultravioleta que puede ser perjudicial para los ojos No mire fijamente a la l mpara Al examinar la radiaci n de las l mparas utilice siempre gafas de seguridad que absorban este tipo de rayos 0000000 Rayonnement UV La lampe met un rayonnement UV intense susceptible de provoquer des lesions oculaires Ne pas regard
13. stability drift System Description Transmission 0 to 100 Absorbance 6 000 to 6 000 display range 1 to 9999 concentration units Absorbance 0 003 measured at Absorbance 1 at 440 nm 546 1 nm and 635 nm with NIST 930 filters Absorbance 0 015 measured at Absorbance 1 at 257 nm and 350 nm with potassium dichromate solution Transmission lt 0 02 at 220 nm 340 nm and 370 nm Absorbance gt 2 measured at 200 nm with potassium chloride solution against distilled water Absorbance 0 0005 2 nm slit corrected 200 nm to 1050 nm scan speed 240 nm min smooth 2 2 nm slit Absorbance lt 0 00006 RMS Absorbance lt 0 0002 peak to peak 3 min at Absorbance 0 wavelength 500 nm and response 2 s Absorbance lt 0 0003 per hour 500 nm after warmup B K2C120 7 60 06 pg mL 0 5 in sulfuric acid c H SO4 0 005 mol L B KC1 12 mg mL 4 0 5 47 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Data Output Digital port One RS 232 C interface serial for connecting a PC 48 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com System Description Lambda 45 Specification General Type Scanning double beam spectrometer for the UV Vis range operation by PC Dimensions Width 650 nm Height 260 nm De
14. terminale di terra massa del conduttore di protezione possono rendere pericoloso lo strumento E vietato provocare volontariamente queste interruzioni Risico s door elektriciteit Het instrument moet voor de werking altijd geaard zijn In geen geval mag de aarding van het instrument of de netvoeding worden onderbroken of worden verwijderd Perigo por electricidade Para a opera o o aparelho tem de estar sempre ligado terra De forma alguma separar ou retirar os condutores de protec o a terra no aparelho ou no cabo de alimenta o da rede oe 96 oan 29 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 30 A WARNING W02 02 Lethal voltages are present in the instrument e Even with the power switch OFF line power voltages can still be present within the instrument e When the instrument is connected to line power terminals may be live and opening covers or removing parts except those to which access can be gained by hana is likely to expose live parts e Capacitors inside the instrument may still be charged even if the instrument has been disconnected from all voltage sources R Q Gef hrliche Spannung im Ger t e Auch in ausgeschaltetem Zustand kann an einigen Stellen im Ger t Netzspannung anliegen wenn das Ger t am Stromnetz angeschlossen ist e Auch bei ausgeschaltete
15. the ultraviolet UV range mirror M1 is raised to permit radiation from the deuterium lamp to strike source mirror M2 Source change is automatic during monochromator slewing Radiation from the source lamp is reflected from source mirror M2 through an optical filter on the filter wheel assembly A stepping motor drives the filter wheel to be in sychronization with the monochromator Depending on the wavelength being produced the appropriate optical filter is located in the beam path to prefilter the radiation before it enters the monochromator Filter change is automatic during monochromator slewing Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com System Description From the optical filter the radiation passes through the entrance slit Slit 1 of the monochromator The radiation is dispersed at the grating to produce a spectrum The rotational position of the grating effectively selects a segment of the spectrum reflecting this segment through the exit slit Slit 2 to mirror M3 Halogen lamp M Mirror M1 M4 and M5 Plane Mirror D M2 Toroidal Mirror M2 M3 Spherical Mirror Deuterium lamp NG Ow Filter wheel Reference baa Detector VA Slit 1 M5 Ce D 4 0 M3 Lens Beam Splitter Grating fa Monochromator Lens Detector Sample Figure 6 Optical Path for Lambda 25 35 45 replaced with a pre monochromator in the Lambda
16. utilisons la formule WARNING DANGER pour avertir des situations pouvant occasionner des dommages corporels a l utilisateur ou a d autres personnes Les d tails sur ces circonstances sont donn es dans un encadr semblable a celui ci y Warning Pericolo Con il termine WARNING PERICOLO vengono segnalate situazioni che potrebbero provocare incidenti alle persone Troverete informazioni su tali circostanze in un riquadro come questo Warning Waarschuwing Betekent dat wanneer de genoemde aanwijzing niet in acht wordt genomen dit kan leiden tot verwondingen van de gebruiker o 6 Warning Aviso Significa que a n o observ ncia da instru o referida poder causar um ferimento ao usu rio 26 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Introduction Other Warnings used in this Manual W01 02 Unauthorized Adjustments and Servicing Do not attempt to make adjustments replacements or repairs to this instrument except as described in the accompanying User Documentation Only a PerkinElmer service representative or similarly trained and authorized person should be permitted to service the instrument 0000008 0p Unzul ssige Arbeiten am Gerat Wartungs oder Reparaturarbeiten oder Justierungen die in der Benutzer dokumentation zum Ger t nicht beschrieben sind d rfen nur vom PerkinElmer Kundendienst oder von entsprechend ausg
17. yellow lead of the line power cord is connected to true electrical earth ground If any part of the instrument is not installed by a PerkinElmer service representative make sure that the line power plug is wired correctly Protective Conductor Green Yellow Green earth ground 10 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Warnings and Safety Information Electrical Hazard Any interruption of the protective conductor inside or outside the instrument or disconnection of the protective conductor earth ground terminal is likely to make the instrument dangerous Intentional interruption is prohibited N WARNING Lethal voltages are present in the instrument Even with the power switch OFF line power voltages can still be present within the instrument When the instrument is connected to line power terminals may be live and opening covers or removing parts except those to which access can be gained without the use of a tool is likely to expose live parts Capacitors inside the instrument may still be charged even if the instrument has been disconnected from all voltage sources Connect the instrument to a correctly installed line power outlet that has a protective conductor earth ground Do not attempt to make internal adjustments or replacements except as directed in this handbook Do not operate the instrument with any covers or parts remove
18. 190 210 230 250 270 m 290 310 330 350 370 Figure 28 Lower Wavelength Limits of Solvent 110 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Index Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com 1 Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Index A Accessories 82 Accessory Installation Autosampler 81 General accessory installation 73 Linear Transporter 78 Multi sippers 80 Temperature Sensor 79 B Basic Installation Connecting to the Electrical Supply 59 Laboratory Requirements 55 Single Cell Holder 63 Unpacking and Inspection 58 I Introduction 37 M Maintenance 87 Changing Fuses 103 Cleaning the Sample Compartment 88 Daily Care 87 Obtaining Service 87 Replacing a Lamp 92 Use and Care of Cells 90 O Operating with Accessories 82 General 82 Index R Replacement Parts 105 Requirements for Operation with Accessories 83 A Safety information Electromagnetic Compatibility EMC 13 Safety Information in the Handbook 9 Safety Information 9 CSA Compliance 9 Electrical Protection 9 Electrical Safety 10 Environment 14 IEC 1010 Compliance 9 Solvent Properties 111 Startup and Shutdown 6 Shutdown 62 Startup 6 System Description Features 37 Optical System Lambda 20 38 T Technical
19. 45 Y _ selectable on the Lambda 35 45 39 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide The exit slit restricts the spectrum segment to a near monochromatic radiation beam The slits provide a spectral bandpass of 1 nm on the Lambda 25 and are selectable to be 0 5 1 2 or 4 nm on the Lambda 35 or 45 From mirror M3 the radiation is reflected onto a beam splitter which allows 50 of the radiation to pass onto plane mirror M4 and reflects 50 of the radiation onto plane mirror MS Mirror M4 focuses the radiation beam in the sample cell The beam then passes through a convex lens onto the photodiode detector Mirror M5 focuses the radiation beam in the reference cell The beam then passes through a convex lens onto the photodiode detector 40 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com System Description Lambda 25 Specification General Type Scanning double beam spectrometer for the UV Vis range operation by PC Dimensions Width 650 nm Height 260 nm Depth 560 nm Ambient operating temperature 15 C to 35 C Humidity range 20 to 80 relative humidity without condensation Technical Standard In compliance with the requirements for technical instruments stipulated by IEC 61010 1 1990 and amendments A1 1992 and A2 1995 Radio interference suppression In co
20. Data Lambda 20 41 Lambda 40 45 U Unusual Samples 109 113 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 114 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com
21. E PELTIER J30 Accessory O CS O panel coooao J1 6010000600000 0000600000000 O O AUTOSAMPLER Sandra Ol configuration ACCY SPHERE J88 P C O 00000 SADO OO 00000 OI ooooo Dodo 0000 eee Figure 12 Accessory Connector Panel 74 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Accessories Designation Connector for Remarks Accessory Port Accy Linear transporter multi sipper PC bus interface PC PC RS 232 C interface J14 13 Cell Changer 13 Cell autochanger Active J10 J11 Temp Temperature sensor J10 2 pin socket J11 4 pin socket J30 Peltier Peltier cell changer Active and Peltier cell holder J88 Sphere Integrating Sphere Active J1 Pump for dissolution Active Autosampler AS 93plus RS 232 C Autosampler Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com 75 Lambda 25 35 45 User s Guide Accessory Connections Overview S Peltier Cell Changer OA Temperature Sensor AS 93plus Autosampler Peltier Cell Holder AUTOSAMPLER OCO SPHERE J88 oo T O Integrating t Linear Transporter Multi sipper Figure 13 Accessories for the Lambda 25 35 45 Sample Compartment Cover Some of the accessories used with the spectrometer require removal
22. KET og stremforbindeiseme skal v re skilt inden De opretter eller ndrer noget i de elektriske forbindelser Peligro el ctrico Para evitar posibles da os personales y al equipo apague todos los m dulos del sistema y descon ctelos de la toma de corriente antes de establecer nuevas conexiones el ctricas o de modificar conexiones existentes Risque d lectrocution Afin d viter des blessures ventuelles et des d t riorations de l instrument mettre tous les instruments du syst me hors tension et les d connecter de l alimentation par le secteur avant de modifier des connexions lectriques ou d en r aliser de nouvelles Pericolo elettricit Per evitare potenziali incidenti alle persone e danni allo strumento mettere in posizione OFF l interruttore di tutti gli strumenti del sistema e scollegarli dalla fonte di alimentazione prima di modificare i collegamenti elettrici o di creare nuovi collegamenti Risico s door elektriciteit Om het gevaar van letsel door elektrische spanning en het gevaar voor beschadiging van het instrument te voorkomen schakelt u voordat u welke elektrische verbinding dan ook wilt maken of wilt veranderen alle instrumenten van de meetplaats UIT en ontkoppelt u de netvoeding Perigo por electricidade A fim de evitar o perigo de ferimento por tens o el ctrica e o perigo de danifica o dos aparelhos DESLIGUE todos os aparelhos do posto de medi o e interrompa as liga es
23. Lambda 25 35 45 User s Guide Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Release History Part Number Publication Date 09934422 August 2000 B September 2000 User Assistance PerkinElmer Ltd Post Office Lane Beaconsfield Buckinghamshire HP9 1QA Printed in the United Kingdom Notices The information contained in this document is subject to change without notice PerkinElmer makes no warranty of any kind with regard to the material including but not limited to the implied warranties of merchantability and fitness for a particular purpose PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing performance or use of this material Copyright Information This document contains proprietary information that is protected by copyright All rights are reserved No part of this publication may be reproduced in any form whatsoever or translated into any language without the prior written permission of PerkinElmer Inc Copyright O 2000 PerkinElmer Inc Trademarks Registered names trademarks etc used in this document even when not specifically marked as such are protected by law UV WinLab is a trademark of PerkinElmer Inc PerkinElmer is a registered trademark of PerkinElmer Inc Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientifi
24. RCE www artisan scientific com Maintenance Maintenance Obtaining Service All internal servicing of the instrument should be performed by a PerkinElmer service representative or similarly authorized person Please contact your local PerkinElmer sales or service office to obtain service Maintenance procedures that you can perform yourself are described in this chapter Unauthorized Adjustments and Servicing Do not attempt to make adjustments replacements or repairs to this instrument except as described in the accompanying User Documentation Only a PerkinElmer service representative or similarly trained and authorized person should be permitted to service the instrument Daily Care Switch off the instrument before cleaning any spilled materials The instrument is constructed with high quality components and requires little maintenance other than to keep it clean and free of dust To protect the optical system from dust and fumes you should keep the sample compartment cover closed except for when you are carrying out work in the compartment 87 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 88 You should observe the following care routine to maintain your instrument in good condition CAUTION Immediately clean all spilled materials from the affected area and wipe it dry with lintless paper or cloth If the s
25. ample compartment windows have to be wiped make sure scratches are not introduced Sample windows are optical components and you should handle them in the same way as high quality cells Do not leave samples particularly those given to fuming or evaporation in the sample compartment for longer than necessary If any type of sample handling system is installed and portions of it are left in the sample compartment such as a sipper and flowcell make certain that the system is cleaned at the end of the working day Generally such systems should be filled with deionized water when left overnight Risk of damage to Optics or Electronics Take care not to spill liquids onto the spectrometer Expensive damage can result to the optics or electronics if liquids are spilled and run inside the instrument or onto the keyboard Cleaning the Sample Compartment You must clean the sample compartment every time anything is spilled into it This preserves the matt black finish and prevents corrosion and contamination The standard sample compartment baseplates have drain holes in them to run off spilled liquids to the benchtop underneath the instrument You can place a sheet of thick filter paper under the instrument if required First remove the cell holder or other sample handling accessory from the sample compartment Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Maintenanc
26. arning To avoid electrical shock disconnect power cord before servicing Warning Hot Lamp Warning UV Light Source Wear Protective Glasses When Working With Lid Open Figure 4 Warnings on the inside of the lamp compartment 19 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 20 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Introduction 2 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com 1 Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Introduction About this Manual This manual contains the following sections e Warnings and Safety Information e Introduction e System Description e Installation e Accessories e Maintenance e Analytical Notes 23 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 24 NOTE Conventions Used in this Manual Normal text is used to provide information and instructions Bold text refers to text that is displayed on the screen UPPERCASE text for example ENTER or ALT refers to keys on the PC keyboard is used to show that you have to press two keys at the same time for example ALT F Definitions
27. avelength range Scan speeds 7 5 15 30 60 120 240 480 960 1920 and 2880 nm min 50 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Ordinate Photometric range Photometric accuracy Stray radiation Baseline linearity Baseline noise Baseline stability drift System Description Transmission 0 to 100 Absorbance 6 000 to 6 000 display range 1 to 9999 concentration units Absorbance 0 003 measured at Absorbance 1 at 440 nm 546 1 nm and 635 nm with NIST 930 filters Absorbance 0 015 measured at Absorbance 1 at 257 nm and 350 nm with potassium dichromate solution Transmission lt 0 02 at 220 nm 340 nm and 370 nm Absorbance gt 2 measured at 200 nm with potassium chloride solution against distilled water Absorbance 0 0005 2 nm slit corrected 200 nm to 1100 nm scan speed 240 nm min smooth 2 2 nm slit Absorbance lt 0 00006 RMS Absorbance lt 0 0002 peak to peak 3 min at Absorbance 0 wavelength 500 nm and response 2 s Absorbance lt 0 0003 per hour 500 nm after warmup B K2C120 7 60 06 pg mL 0 5 in sulfuric acid c H SO4 0 005 mol L B KC1 12 mg mL 4 0 5 51 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Data Output Digital port One RS 232 C in
28. c com Contents Contents A NO 3 Warnings and Safety Information oomcoonconncoonnsnnsonssss 7 SE Ve LE 9 Safety Information in the Handbook oooonocinociniconoconocnnonnonnnonanonannonnnonoos 9 TEC 61010 Compliance ooooocccoccnococonoconoconoconnnonnnnnnnnnn nono nono ncon ccoo nncnnncnnnnns 9 AM A sea sdnestbneadadensasdeaannavans 9 Electrical Protection iii ia 9 tee HE bi scaas 10 EMC CompldtiCS viii ida t GANG 13 EC Enter cit ii NANANA GAD NHA Aap AD 13 FCC rules and regulations ccccccccssecesecesecesecseeceeeeeneeeeeeseeeseeeeeneesaes 13 ENVIO ME ii ld R 14 Lifting the Lambda 2 5 diia 17 Dada iii 18 Introduction oomcooncooncooncnoncnonconnnonaconaconanonnnonnnconaconaconnccnncconoconccono cono cocconnoos 21 About this Mania vincia 23 Conventions Used in this Manual 24 IR dee EE 24 Notes cautions and warnings ccccccccesccesecessceeeceeeeeeeeeseeeseeeeeneeeneeaes 24 Other Warnings used in this Manual 27 System Description ccscccccsscscsecssccecsscccscccsesccesssccessccsescecsescessscseeseees 35 reet e E 37 ECM ri a ear 37 Optical System of the Lambda 25 35 A5 38 Lambda 25 Specifications 1 4a GATA GARA NGANN 41 General eier 41 OD ia an 42 ABSCISSA tido OR dt nao 42 Ordinarias 43 Data Output aida 44 Lambda 35 Specrti Cation iva ccscsvesisidees ss saaacete NAGANA 45 Generala pA Ee 45 AA 46 ADSCIS aaa IR IBINUKA E 46 ONG ALES as RO 47 Artisan Scientific Quality Instrum
29. connections WARNING Installation Overview Installation See linear transporter manual Cables Lead through bottom of spectrometer housing Tubes Lead through the tube ports Sample Compartment Cover Unchanged Alignment See linear transporter manual 78 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Accessories Temperature Sensor Electrical hazard To prevent potential injury to yourself and damage to the instrument switch OFF all instruments in the system and disconnect them from the line power supply before you alter or make any new electrical connections WARNING Installation Overview NOTE When connecting the temperature sensor align the red mark on the plug with the red mark on the socket Pull the collar on the plug back to connect disconnect the plug Release the collar to secure the plug see Figure 2 page 74 Connector on Spectrometer Temp J10 Installation See temperature sensor description Sample Compartment Cover Unchanged Cables Lead through the tube ports 79 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Multisippers Electrical hazard To prevent potential injury to yourself and damage to the instrument switch OFF all instruments in the system and disconnect them from the line power supply before you alter or
30. cscssssssscssscssssssscssscssssessessnesssessssssesssessscssssoesses 105 Unusual Samples coria DAANAN NA cadavers detr s 107 Volatile Samples ia 107 Samples not Governed by the Beer Lambert Lawin 107 Chemically Reactive Samples 108 Photoactive Samples 108 Other Sample Properti8S ooonoconncnnnnononncoonononconnconnonnononocon ccoo nono noconanos 108 Solvent Properties ed bet Eeer 109 Index AA ssss reis 111 Indek ei a a EE EEE E E O E EE EOE 113 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Warnings and 4 Safety Information Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com 1 Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Warnings and Safety Information Safety Information Safety Information in the Handbook This handbook contains information and warnings that must be followed by the user to ensure safe operation and to maintain the instrument in a safe condition Possible hazards that could harm the user or result in damage to the instrument are clearly stated at appropriate places throughout this handbook Before using the instrument it is essential to read the manual carefully and to pay particular attention to a
31. d Servicing should be carried out only by a PerkinElmer service representative or similarly authorized and trained person Disconnect the instrument from all voltage sources before opening it for any adjustment replacement maintenance or repair If afterwards the opened instrument must be operated for further adjustment maintenance or repair this must only be done by a skilled person who is aware of the hazard involved 11 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Use only fuses with the required current rating and of the specified type for replacement Do not use makeshift fuses or short circuit the fuse holders Whenever it is likely that the instrument is no longer electrically safe for use make the instrument inoperative and secure it against any unauthorized or unintentional operation The instrument is likely to be electrically unsafe when it Shows visible damage Fails to perform the intended measurement Has been subjected to prolonged storage under unfavorable conditions e Has been subjected to severe transport stresses If the equipment is used in a manner not specified herein the protection provided by the equipment may be impaired WARNING 12 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Warnings and Safety Information EMC Com
32. dling rules to prevent analytical errors and to achieve utmost precision e Only hold cells by non optical surfaces such as the matt finish surfaces e Protect cells from scratches and never permit them to rub against one another or against other hard surfaces e Avoid abrasive corrosive or stain producing cleaning agents and make certain that the exposed surfaces of cells are optically clean e Always wipe the optical surfaces of cells dry and free of fingermarks using a soft cloth or cleaning tissue just before placing them in the cell holder e When measuring cold solutions always bear in mind that condensation can form on the optical surfaces e Make certain no bubbles cling to the inner surfaces of the cell particularly when handling cold solutions e For maximum precision and accuracy calibrate and test with cells of the same type and always insert cells into the holders with the same orientation Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Maintenance Pressure Buildup in Cells If you are using stoppered cells observe the following rules to prevent the buildup of internal pressure that could cause the stopper to pop out of the cell e Only fill the cell so full that the liquid meniscus is just above the radiation beam The remaining air space in the cell is then adequate to compensate for any slight increase in pressure in the cell during routine o
33. do em atmosferas explodiveis Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Introduction W02 01 Electrical Hazard Any interruption of the protective conductor inside or outside the instrument or disconnection of the protective conductor earth ground terminal is likely to make the instrument dangerous Intentional interruption is prohibited Gef hrdung durch Elektrizitat Das Ger t muB zum Betrieb immer geerdet sein Auf keinen Fall die Schutzleiter im Ger t oder in der Netzzuleitung trennen oder entfernen Fare p grund af elektricitet Apparatet skal altid veere jordet Man m under ingen omst ndigheder skille eller fjerne jordlederen inde i apparatet eller i str mledningen Peligro el ctrico Cualquier interrupci n del conductor de protecci n dentro o fuera del aparato o la desconexi n del terminal del mismo toma de tierra podr an ocasionar serios peligros al usar el equipo Prohibida la interrupci n intencionada Risque d lectrocution Toute interruption du conducteur de protection a l int rieur ou a l ext rieur de l instrument ou d connexion du raccord du conducteur de protection terre peut rendre l instrument dangereux H est interdit d interrompre volontairement ce conducteur Pericolo elettricita Qualsiasi interruzione della protezione del conduttore all interno o all esterno dello strumento o lo scollegamento del
34. e 2 Using a soft cloth and mild laboratory detergent solution lightly scrub away all foreign material 3 Using a clean cloth dampened with water rinse the cleaned surfaces thoroughly 4 Dry with lint free cloth or tissue Sample Compartment Window Windows are provided with the spectrometer The window is made of silica and may be used in the entire spectral range of the spectrometer The window seals the sample compartment and thus protects the instrument s optics from dust and fuming or aggressive samples e Generally the window should be installed at all times e The window is an optical component and requires the same care and handling as cells e You can remove the window to clean it The frame is magnetic and can be removed by hand Windows are most suitably cleaned by wiping them with a soft cloth moistened with ethanol 89 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 90 Use and Care of Cells Cell Handling A good spectrometer cell is an optical device forming a part of the optical system of the instrument with which it is used It must be accorded the same careful treatment applied to any optical component Optical faults of a minor nature for example scratches lint fingermarks on the optical surfaces can easily introduce substantial analytical errors You should observe the following list of cell han
35. e blank reduces the reference energy thus increasing noise e Evaporation should be fairly low at ambient temperature In general aromatic compounds exhibit high absorption in the UV region and hence are not suitable as solvents for measurements in this region Water is virtually the only useful solvent below 195 nm but it must be freed from oxygen to attain best transmission Whenever you are going to use a solvent with unknown absorption characteristics scan its spectrum first to determine whether it is suitable The lower wavelength limits of a number of commonly used solvents are presented in the following table The lower limit has been defined as that wavelength at which 10 mm of pure solvent has a transmission of 10 109 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Acetone samme Tetrachloroethylene m Xylene Toluene Benzene N N Dimethylformamide Ethyl Propionate Carbon Tetrachloride Ethyl Formate Buty Acetate Methyl Form Chloroform 1 2 Dichloroethane Dichloromethane Glycerol Dioxan cmt a A SA H exane SITTITTT TA ee iOctane coy O ee ee 2 2 4 Trimethylpentane CN AN NY Acetonitrile kd tee TTE EET Cyclohexane EXA TNA ANNA Methanol manerer ee IAN Ethanol Esser Methylcyclohexane i Propanol Water
36. ebildeten und autorisierten Fachkr ften ausgef hrt werden Ikke tilladte arbejder p apparatet Vedligeholdelses eller reparationsarbejder eller justeringer som ikke er beskrevet i dokumenterne til brugeren m kun udf res af PerkinElmers kundeservice eller af tilsvarende uddannede og autoriserede fagfolk Ajustes y servicios sin autorizaci n No intente realizar ning n tipo de ajuste sustituci n o reparaci n en este aparato a excepci n de lo descrito en la Documentaci n del Usuario que se adjunta Tan s lo un ingeniero de Servicio T cnico de PerkinElmer o una persona de formaci n y autorizaci n similares podr n realizar trabajos de revisi n y mantenimiento delinstrumento R glages et entretien non autoris s Ne pas essayer d effectuer des r glages des remplacements ou des r parations sur cet instrument d une mani re autre que celle d crite dans la Documentation Utilisateur jointe Seul un repr sentant du service apres vente PerkinElmer ou une personne autoris e et de m me formation devraient tre autoris s intervenir sur l instrument Regolazione e manutenzione non autorizzate Non tentare di regolare sostituire o riparare questo strumento se non come descritto nell allegata Documentazione Utente Solo un rappresentante dell assistenza PerkinElmer o una persona dalla analoga esperienza pu eseguire se autorizzata la manutenzione dello strumento Niet toegestane werkzaamheden aan het instrument Onder
37. entation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Lambda 45 Specification ccccccccssecsceceseceseceeeceeseceseceeeseeeseeeeeseeeeeeneeeaes 49 MONS CG 49 Bel 50 E 50 Or imate ET 51 Data Output ia mna NN KANG GANAN AN AA 52 usteet GN DANARIIDA KITAKITS 53 Preparing the Working Aren 55 Equipment Provided rierien rn E E R EA EEE RE 57 e EE EE 57 ele AA E ETRE 57 Unpacking your Instrument 58 Unpacking and Inspection cccccccesecessceseceseceeeeeeeeeeeeeeneesaeenseeseneeeaes 58 Connecting to the Electrical Supply nono nono nonnnnonnnos 59 Setting up the PC ass 60 Connecting the PC to the spectrometer oooooonnconoconocccnoncnononnnonnnnnonanannnos 60 Installing UV Wm bhabi a AGAIN KAKAYANIN 60 Startup and Sbutdouwm maana snan ccoo nocnnoconoconn nn rnnnnrnnnnonnnonnnrannos 61 A BE 61 SUI idad 62 Installing the Single Cell Holder 63 Description sas 63 ACCESSOTI S ciicccssecisadescesssussescessassecesesvoccsesscacsecstnsesdsacsses cosccesuecsucessecsctesouress s 71 Accessory Installaton 73 Accessory Connector Panel 74 Accessory Connections Uverview nananana saaan anna 76 Sample Compartment Cover u s 76 Sample Compartment Front Cover 77 Sample Compartment Window oooconoccnocnnocnnonnnonnnono nono nono nono ncnno nono ncnnnnos 77 Linear Transporter 78 Installation Overview ccsccesccessceseceeeeeeeceeseceseceeeeseeeseeeseseesaeeseeeseneena
38. er fixement une lampe allum e Toujours porter des lunettes de protection absorbant les UV pour regarder le rayonnement des lampes Radiazioni ultraviolette La lampada emette intense radiazioni ultraviolette dannose per gli occhi Non fissare le lampade accese indossare sempre occhiali protettivi ad assorbimento degli ultra violetti quando si guardano le radiazioni emesse dalla lampade UV straling Risico s voor de ogen De stralingsbron lamp emitteert intensieve UV straling en kan daardoor letsel aan de ogen veroorzaken Draag een veiligheidsbril die de ge mitteerde UV straling voldoende absorbeert wanneer u in de brandende stralingsbron moet kijken Radia o UV perigo para os olhos A fonte de radia o l mpada emite uma radia o UV intensapodendo assim provocar ferimentos aos olhos Use culos de protec o que absorvam suficientemente a radia o UV emitida ao olhar directamente para a fonte de radia o luminosa 33 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 34 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com System Description 3 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com 1 Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scien
39. es 78 Temp rat re DENSO D3 ADA LIGA 79 Installation OVETVIEW 0 cccceeseeesceseseeeeceseceneeseceeeeaeesecaesaeeeeceaeeeeeeaeenee 79 Wi Dt E 80 Installation Overview 0 ecceceeseeeeceseeseeeeceseceneeseceeeeaeesecaeeaeeeeeaeeeneeaeenee 80 A tosamplet EE 81 Operating with Accessories oooooccoocnoonnoococonnnnononononononnn nono nono ronn crono none nana nnonnnos 82 CSM STA os eas O 82 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Contents EERSTEN Naa eege ee ege 82 Requirements for Operation with Accessories ccceseeseeeeeteeeeeeeenee 83 Maintenance sccccscsssccsscsssesssessscessesssssscscssscsssssssssssssssssecssssssesessoessoeese 85 Maintenant AA ERER 87 Obtaining Sevi dise 87 IR 87 Cleaning the Sample Compartment nono nconnconnnos 88 Use and Care of Cells 0n NABANG KIA NIBAMAAAAR E 90 Cell Handling e 90 Pressure Buildup in Cells ooooonconinocinccnonanonnconaconnnonncnnnnnnn oran ron ronnronnno 91 Replacing a LAMP ana KUGITA IANG EEN 92 Halogen Lamp Replacement cccccescccsseceseceeeeeseeeeeeeeeseeeseeeeeneeaes 93 Deuterium Lamp Replacement crono nonnnnnrannnannno 96 Lamp Energy Attenuator cccccecsscceesceceseceeseeceeaeeceeneeceaeeceeateceeneessas 99 Lamp Alignment Procedure ooooonocccoconocnnonnnonnnonnnonnnonononancnnn nono noanncinnnos 101 Changing Fusil iii 102 Replacement E EE 104 Analytical Notes sccccc
40. fuse holder at the rear of the instrument Fuse Holder a Figure 26 Rear view fuse holder 1 Switch off the instrument and remove the line power cord from the electrical supply 2 Squeeze the two lugs at each side of the fuse holder and gently pull out Fuse Holder Figure 27 Removing the fuse holder 102 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Maintenance 3 Replace the spent fuse with a new one of the same type and rating 100 V 120 V 63AT 210 V 240 V 3 15 AT NOTE The module has two fuses Although only one fuse may have blown both fuses should be replaced at the same time 4 Replace the fuse holder Align the lug at the bottom of the fuse holder with the slot in the socket A click is heard as each lug snaps into place NOTE f you use the correct fuses but the instrument still does not work correctly or the fuses blow repeatedly contact your PerkinElmer office or representative 103 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Replacement Parts Supplies accessories and replacement parts can be ordered directly from PerkinElmer e ssentials PerkinElmer s catalog service offers a full selection of high quality ultraviolet fluorescence and polarimetry supplies through the Supplies Catalog for Ultraviolet Visible and Fluo
41. h the legal requirements of the EMC directive 89 336 EEC EN 61326 45 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Optics Beam center height 15 mm above cell holder bottom Beam cross section 0 5 nm slit ca 0 25 mm x 7 mm width x height 1 nm slit ca 0 5 mm x 7 5 mm width x height 2 nm slit ca 1 mm x 7 5 mm width x height 4 nm slit ca 2 mm x 7 5 mm width x height at focal point of sample and reference beam in sample compartment Optical pathlength in 121 mm sample compartment Grating Holographic concave grating with 1053 lines mm in the Monochromator center Prealigned deuterium and halogen lamps Detector Photodiodes One for the sample beam and one for the reference beam Abscissa Wavelength range 190 nm to 1100 nm 0 nm for alignment purposes E accuracy 0 1 nm Wavelength reproducibility reproducibility 0 1nm 0am 1 nm Spectral bandwidth 0 5 nm 1 nm 2 nm 4 nm selectable fixed slit widths Lamp change Automatically at 326 nm software selectable over the whole wavelength range Scan speeds 7 5 15 30 60 120 240 480 960 1920 and 2880 nm min 46 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Ordinate Photometric range Photometric accuracy Stray radiation Baseline linearity Baseline noise Baseline
42. he single cell holder 3 Move the milled posts a little to locate the threaded holes in the baseplate and then tighten the milled posts The tube ports located at the front of the sample compartment allow you to lead tubes from flowcells water thermsotatted cell holders etc in and out of the sample compartment When not in use you should always insert the caps into the ports Aligning the Single Cell Holder Coarse alignment of the single cell holder is carried out as follows 1 Start the system as described in Startup on page 61 2 Open the sample compartment cover 3 Fill matching cells with a low absorbing solvent deionized water or ethanol 4 Insert one cell into the sample cell holder and one into the reference cell holder Make certain that the cell is pushed down fully NOTE The alignment procedure is for a given cell in a given holder After alignment the cell should always be used in the same holder 65 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 66 3 10 11 12 Block the sample and reference beam window on the right hand side of the sample compartment with a card to prevent white light from saturating the detector Return to standby display From the Application menu in UV WinLab select Manual The lambda Manual Control dialog is displayed Set the Wavelength to 0 nm and click Setup By holdi
43. houds reparatie of afstelwerkzaamheden die niet in de gebruikersdocumentatie van het instrument worden omschreven mogen alleen door personeel van de klantenservice of door overeenkomstig geschoolde en geautoriseerde vakmensen worden uitgevoerd Trabalhos n o permitidos no aparelho Trabalhos de manuten o ou de repara o ou ajustamentos nao descritos na documenta o do usu rio para o aparelho apenas podem ser executados pelo Servi o T cnico da PerkinElmer ou por pessoal especializado devidamente formado e autorizado Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com 27 Lambda 25 35 45 User s Guide 28 W01 03 i Explosive Atmosphere This instrument is not designed for operation in an explosive atmosphere 30000808 Explosionsf hige Atmospharen Das Ger t darf nicht in explosionsfahigen Atmosph ren betrieben werden Eksplosive omgivelser Apparatet m ikke anvendes i eksplosive omgivelser Atm sfera explosiva Este aparato no ha sido dise ado para utilizarlo en atm sferas explosivas Atmosph re explosive Cet instrument n est pas con u pour fonctionner dans une atmosph re explosive Atmosfera esplosiva Questo strumento non adatto per l uso in atmosfera esplosiva Explosiegevaarlijke omgevingen Het instrument mag niet in een explosiegevaarlijke omgeving worden gebruikt Atmosferas explodiveis O aparelho nao pode ser utiliza
44. l e Mains supply fluctuations not exceeding 10 of the nominal voltage 14 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Warnings and Safety Information When you remove the instrument from storage before putting it into operation allow it to stand for at least a day under the approved operating conditions Chemicals Use store and dispose of chemicals that you require for your analyses in accordance with the manufacturer s recommendations and local safety regulations Hazardous Chemicals A Some chemicals used with this instrument may be hazardous or may 4 become hazardous after completion of an analysis WARNING The responsible body for example Laboratory Manager must take the necessary precautions to ensure that the surrounding workplace and instrument operators are not exposed to hazardous levels of toxic substances chemical or biological as defined in the applicable Material Safety Data Sheets MSDS or OSHA ACGIH or COSHH documents Venting for fumes and disposal of waste must be in accordance with all national state and local health and safety regulations and laws OSHA Occupational Safety and Health Administration U S A ACGIH American Conference of Governmental Industrial Hygienists U S A COSHH Control of Substances Hazardous to Health U K Toxic Fumes If you are working with volatile solvents or toxic substances you must
45. le Cell Holder B0505071 63 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Inscription legible Use in Spectrometer on Holder LAMBDA In this position the cell holder can be used with all Lambda Series Spectrometers BIO LAMBDA 2 The cell holder should be used in this position with Lambda 25 35 45 Series Spectrometers The smallest beam diameter is exactly in the middle of the cell This is useful especially for operation with micro and semi micro cells Installing the Cell Holder NOTE Depending on the spectrometer the single cell holder can be installed in two different positions in the sample compartment Always install the holder such that the arrow on the cell holder lines up with the center point on the baseplate Install the single cell holder in the sample compartment as follows 1 Orientate the holder so that the lifter is toward the rear of the sample compartment 2 Lower the holder so that the two alignment holes slip onto the two studs on the baseplate at the bottom of the sample compartment The arrow on the cell holder must line up with the center point of the baseplate and BIO LAMBDA 2 must be legible 64 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Installation Center Point Tube Ports Leg els Figure 10 Installing t
46. loten op sommige plaatsen in het instrument netspanning staan e Ook bij een uitgeschakeld instrument en een onderbroken netvoeding kunnen condensatoren in het instrument nog een gevaarlijke lading bevatten Tens o perigosa no aparelho perigo de ferimento e Mesmo desligado o aparelho poder ainda ter tens o de rede em alguns pontos enquanto estiver ligado a rede de corrente e Mesmo com o aparelho desligado e a ligagao a rede de corrente interrompida os condensadores dentro do aparelho ainda poder o ter ums tens o perigosa aplicada H Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com 31 Lambda 25 35 45 User s Guide W02 03 Electrical Hazard To prevent potential injury to yourself and damage to the instrument switch OFF all instruments in the system and disconnect them from the WARNING line power supply before you alter or make any new electrical connections gt Gefahrdung durch Elektrizitat Um Verletzungsgefahr durch elektrische Spannung und Beschadigungsge fahr der Ger te zu vermeiden schalten Sie alle Ger te des MeBplatzes AUS und trennen Sie die Netzverbindungen bevor Sie irgendwelche elektrischen Verbindungen herstellen oder ndern Fare p grund af elektricitet For at undg faren for kveestelser fra den elektriske speending og faren for beskadigelse af apparaterne skal alle apparater p m lepladsen veere SLUK
47. ly 26 Kg we recommend that the instrument is lifted by 2 adults and that it is lifted by the base of the instrument 17 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Labels Labels are fixed to the Lambda 25 35 45 in the locations shown in Figure 1 and Figure 3 Figure 1 Labels on the spectrometer housing Serial number Electrical warnings Serial number inside the sample compartment The following electrical warnings are shown on the rear of the instrument Warning To avoid electrical shock disconnect power supply before changing fuse Disconnect supply when ever cover is removed Warning Grounding circuit continuity is vital for safe operation of equipment Never operate equipment with grounding conductor disconnected FUSE S 100 240 VAC 50 60 Hz 3 15 AMP TYPE T 250V MAX POWER 250 VA Warning For protection against fire hazard replace only with same type and rating of fuse Figure 2 Warning labels on the rear of the instrument 18 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Warnings and Safety Information Warning labels shown on the inside of the lamp compartment Figure 3 Lambda 25 35 45 Spectrometers warning labels inside of lamp compartment The following warnings are shown on the inside of the lamp compartment W
48. m Ger t und getrennter Netzverbindung k nnen Kondensatoren im Ger t noch mit gef hrlicher Spannung geladen sein Farlig sp nding i apparatet fare for kv stelser e Ogs i slukket tilstand kan der v re netsp nding nogle steder i apparatet hvis apparatet er tilsluttet til str mnettet Selv n r apparatet er slukket og str mforbindelsen er afbrudt kan kondensatorerne i apparatet v re ladet med farlig sp nding En el aparato existen voltajes letales e Incluso con el interruptor desconectado puede haber voltaje dentro del equipo e Cuando el instrumento se encuentre conectado a la red el ctrica los terminales pueden estar bajo corriente y stos quedar expuestos al abrir las cubiertas o al extraer componentes exceptuando aquellos a los cuales se puede acceder con la mano e Los condensadores internos del aparato pueden permanecer cargados incluso cuando el aparato haya sido desconectado del voltaje de la l nea Pr sence de tensions mortelles dans l instrument e Meme lorsque l interrupteur de puissance est sur ARRET des tensions de secteur peuvent encore tre pr sentes dans l instrument e Lorsque l instrument est reli au secteur les raccords peuvent tre sous tension et des parties sous tension peuvent tre d couvertes en ouvrant des capots ou en retirant des pieces a exception de celles auxquelles il est possible d acc der manuellement e Les condensateurs contenus dans l instrument peuvent encore tre
49. mpliance with the legal requirements of the EMC directive 89 336 EEC EN 61326 41 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Optics Beam center height 15 mm above cell holder bottom Beam cross section 1 nm slit ca 0 6 mm x 9 mm width x height at focal point of sample and reference beam in sample compartment Optical pathlength in 121 mm sample compartment Grating Monochromator Holographic concave grating with 1053 lines mm in the center Prealigned deuterium and halogen lamps Detector Photodiodes One for the sample beam and one for the reference beam Abscissa 0 nm for alignment purposes Lamp change Automatically at 326 nm software selectable over the whole wavelength range Scan speeds 7 5 15 30 60 120 240 480 960 1920 and 2880 nm min 42 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Ordinate Photometric range Photometric accuracy Stray radiation Baseline linearity Baseline noise Baseline stability drift System Description Transmission 0 to 100 Absorbance 6 000 to 6 000 display range 1 to 9999 concentration units Absorbance 0 003 measured at Absorbance 1 at 440 nm 546 1 nm and 635 nm with NIST 930 filters Absorbance 0 015 measured at Absorbance 1 at 257 nm and 350 nm with po
50. nents of the system make certain that all power switches are set to off then connect the line cords to the electrical power supply The power switch is located at the top right hand rear of the spectrometer NOTE To prevent interferences caused by earth loops when operating with ancillary instruments autosamplers etc connect all components of the system to the same phase of the electrical supply via a multisocket distributor 59 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Setting up the PC The Lambda 25 35 45 range requires a PC running UV WinLab version 2 85 or above Connecting the PC to the spectrometer Connect the PC to the spectrometer using the cable provided 09410022 as shown in Figure 8 PC RS232 COM1 2 09410022 supplied Figure 8 Connecting the PC to the spectrometer Installing UV WinLab To install UV WinLab on the PC follow the instructions in the UV WinLab Software User s Guide 60 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Installation Startup and Shutdown Before starting analysis leave the spectrometer switched on for approximately 10 minutes to allow the lamps to warm up and stabilize Wait until all initialization is complete before starting UV WinLab This will take approximately 3 minutes from switch on
51. ng a piece of matt white paper behind each cell holder visually examine the light spot to see that the radiation beam is passing through the cell sample area Diffraction patterns become apparent if the radiation beam impinges on the cell wall If the radiation beam is not centered exactly loosen the two locking screws and the two milled posts on the relevant cell holder and shift the cell holder plate to center the radiation beam horizontally Then retighten the two milled posts and the two locking screws Now visually check the vertical alignment of the radiation beam in the cell sample area Alignment is correct when the radiation beam is just above the floor of the cell sample area min 2 mm or covers the cell window NOTE The center of the window for micro flowcells should be ideally approximately 15 mm above the base of the cell Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Installation Min 2 mm Figure 11 Correct Alignment of the Radiation Beam in the Cell Sample Area 13 If vertical alignment is required turn the vertical adjustment screw on the lifter either clockwise to raise the cell or counterclockwise to lower the cell 14 Recheck the horizontal alignment of the radiation beam through the cell and correct if necessary 15 From the Application menu in UV WinLab select Manual The Lambda Manual Control dialog is displayed 16 Set the Wavele
52. ngth to any value above 200 nm and click Setup 17 Remove the card blocking the sample beam window and close the sample compartment cover This completes the coarse alignment of the cell holder If necessary proceed with the fine alignment as described below Fine Alignment If fine alignment is necessary proceed as follows 1 From the Application menu in UV WinLab select Manual The Lambda Manual Control dialog is displayed 2 Set the Wavelength to your measurement wavelength or to 460 nm and click Setup 67 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 68 3 10 11 In the UV WinLab Manual control dialog select transmission 46T as ordinate and click Setup Open the sample compartment cover Insert the cell with a low absorbing solvent into the sample cell holder Leave the reference cell holder empty Make horizontal fine alignment to the sample cell holder locking screws and milled posts loosened to obtain the highest possible transmittance reading on the display close sample compartment cover while measuring transmittance Make vertical fine adjustment using the vertical adjustment screw again to obtain the highest possible reading close sample compartment cover while measuring transmittance When you are satisfied with the alignment tighten the milled posts and the locking screws on the cell holder
53. ny advice concerning potential hazards that may arise from the use of the instrument The advice is intended to supplement not supercede the normal safety code of behavior prevailing in the user s country IEC 61010 Compliance This instrument has been designed and tested in accordance with IEC 1010 1 Safety requirements for electrical equipment for measurement control and laboratory use and Amendment to this standard CSA Compliance This instrument meets the Canadian Standards Association CSA Standard CAN CSA C22 2 1010 1 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use General Requirements Electrical Protection Insulation Class I as defined in IEC 61010 1 Installation Category The instruments are able to withstand transient overvoltage according to Installation Category II as defined in IEC 61010 1 and IEC 664 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Pollution Degree The equipment will operate safely in environments that contain non conductive foreign matter and condensation up to Pollution Degree 2 usually only non conductive atmospheric pollution of the equipment occurs occasionally however a temporary conductivity caused by condensation must be expected Electrical Safety To ensure satisfactory and safe operation of the instrument it is essential that the green
54. of the sample compartment cover Do this carefully as follows 1 Open the cover only about 90 degrees to the sample compartment 2 Carefully slide the cover straight up off both hinges to remove To install the sample compartment cover or other accessory cover perform this procedure in reverse 76 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Accessories Sample Compartment Front Cover Some of the accessories used with the spectrometer require removal of the sample compartment front cover Do this carefully as follows 1 Open the sample compartment cover 2 Loosen the two screws securing the front cover of the sample compartment 3 Slide the front cover forwards and remove from the spectrometer To install the sample compartment front cover perform this procedure in reverse Sample Compartment Window To remove or install certain accessories you need to remove the sample compartment windows Each window has a magnetic frame and should be carefully removed by hand 77 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Linear Transporter Electrical hazard To prevent potential injury to yourself and damage to the instrument switch OFF all instruments in the system and disconnect them from the line power supply before you alter or make any new electrical
55. ompartment when the lamps are on Do not gaze into a lighted lamp Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Maintenance 1 Switch off the spectrometer and unplug the line power cord 2 Remove the lamp compartment cover by pressing down the catch and pushing the cover to the left 3 Remove the lamp baffle by slackening the thumbscrews and lifting the lamp baffle vertically upward Thumbscrew Thumbscrew Figure 22 Lamp baffle 4 Unplug the deuterium lamp connector from the terminal board by squeezing in the two lugs at each side of the connector and carefully pulling the connector vertically upward 97 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 98 connector Deuterium lamp Deuterium lamp assembly Figure 23 Deuterium lamp connector 8 Remove the lamp assembly from the bracket by slackening the thumbscrew and pulling the lamp mount vertically upward Save the thumbscrew for use with the new lamp assembly Unpack the new lamp assembly taking care to hold it only by the metal mount to prevent fingermarks on the lamp window Slip the slot at the base of the lamp mount over the stud on the bracket in the lamp compartment and then secure with the thumbscrew Plug the deuterium lamp connector into the socket NOTE The socke
56. peration e If for analytical reasons it is necessary to fill the cell completely insert the stopper only lightly so that the liquid in the cell has a chance to expand e Do not insert a stopper forcefully into a completely filled cell since this is likely to cause the stopper to pop out of the cell e When working at higher temperatures use a drilled stopper 0 4 mm hole to allow for expansion in the cell 91 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Replacing a Lamp Lamp compartment connector Halogen lamp IA assembly e White ceramic Deuterium lamp connector Deuterium lamp assembly Lamp energy attenuator Figure 17 Inside the Lamp Compartment Baffle removed 92 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Maintenance Halogen Lamp Replacement If the lamp burns out or if the bulb becomes blackened after prolonged use you should replace the lamp Replacement lamp assemblies are provided complete with prealigned mounts Part Number B0114620 Figure 18 Prealigned Halogen Lamp B0114620 Electrical Hazard Switch off the spectrometer and remove the plug from the electrical supply before starting with the replacement Risk of Burns WARNING If the old lamp was lighted allow it to cool before proceeding with the
57. pliance EC Directive This product complies with the minimum immunity requirements of IEC 61326 and has been tested to the relevant parts of the following standards IEC 61000 4 2 IEC 61000 4 3 IEC 61000 4 4 IEC 61000 4 5 IEC 61000 4 6 TEC 61000 4 11 This product complies with EN 55011 Group 1 Class A and IEC 61000 3 2 FCC rules and regulations This product is classified as a digital device used exclusively as industrial commercial or medical test equipment It is exempt from the technical standards specified in Part 15 of the FCC Rules and Regulations based on Section 15 103 c 13 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Environment Operating Conditions Explosive Atmosphere This instrument is not designed for operation in an explosive atmosphere WARNING The instrument will operate correctly under the following conditions e Indoors e Ambient temperature 15 C to 35 C e Ambient relative humidity 20 to 80 without condensation e Altitude in the range 0 m to 2000 m The instrument has been designed to be safe under the following environmental conditions e Indoor use e Ambient temperatures of 5 C to 40 C e A maximum ambient relative humidity of 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C e Altitude up to 2000 m above mean sea leve
58. problem Samples not Governed by the Beer Lambert Law Quantitative analyses utilizing the absorption of spectral radiation are based on the Beer Lambert law which states that the absorption is proportional to the concentration of the analyte The law can be expressed in the form A ecd Where A is absorbance e is molar absorption coefficient c is molar concentration d is thickness through which the radiation is transmitted This law is mostly true for dilute solutions but at higher concentrations a plot of absorbance against concentration will be non linear for a number of reasons 107 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 108 The absorption characteristics of a sample can be changed during sample preparation depending on the amount of reagent added for color development and so on For details refer to reference books covering these subjects Temperature has an influence to a greater or lesser degree on the absorption characteristics of a sample You should check this effect if non repeatable results are obtained If you are measuring temperature dependent samples either wait until temperature equilibrium has been attained or use a thermostatted cell or cell holder Chemically Reactive Samples If a reaction takes place in the cell between the sample material and the solvent spectral data based on that sample canno
59. pth 560 nm Ambient operating temperature 15 C to 35 C Humidity range 20 to 80 relative humidity without condensation Technical Standard In compliance with the requirements for technical instruments stipulated by TEC 61010 1 1990 and amendments A1 1992 and A2 1995 Radio interference suppression In compliance with the legal requirements of the EMC directive 89 336 EEC EN 61326 49 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Optics Beam center height 15 mm above cell holder bottom Beam cross section 0 5 nm slit ca 0 25 mm x 7 mm width x height 1 nm slit ca 0 5 mm x 7 5 mm width x height 2 nm slit ca 1 mm x 7 5 mm width x height 4 nm slit ca 2 mm x 7 5 mm width x height at focal point of sample and reference beam in sample compartment Optical pathlength in 121 mm sample compartment Grating Holographic concave grating with 1053 lines mm in the Monochromator center Prealigned deuterium and halogen lamps Detector Photodiodes One for the sample beam and one for the reference beam Abscissa Wavelength range 190 nm to 1100 nm 0 nm for alignment purposes E accuracy 0 1 nm Wavelength reproducibility reproducibility 0 1nm 0am 1 nm Spectral bandwidth 0 5 nm 1 nm 2 nm 4 nm selectable fixed slit widths Lamp change Automatically at 326 nm software selectable over the whole w
60. r firmly onto the pins on the base of the lamp 9 Wipe the bulb with a soft cloth moistened with alcohol to remove dirt since this would otherwise be burned in when the lamp is hot 10 Replace the lamp baffle using the reverse of the procedure described in step 3 11 Replace the lamp compartment cover This completes the halogen lamp replacement procedure 95 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 96 NOTE Deuterium Lamp Replacement If the lamp burns out or indicates falling energy after prolonged use you should replace the lamp Replacement lamp assemblies are provided complete with prealigned mounts Part Number B0160917 aa Operating Hours Counter Figure 21 Prealigned Deuterium Lamp Assembly B0160917 An operating hours counter is incorporated in the red deuterium lamp lead By means of a gap between the two display bars it is possible to read off the number of hours that the lamp has been in operation One scale division corresponds to approximately 100 hours Electrical Hazard Switch off the spectrometer and remove the plug from the electrical supply before starting with the replacement Risk of Burns WARNING If the old lamp was lighted allow it to cool before proceeding with the replacement UV Radiation The lamps emit intense radiation which can damage your eyes Do not open the lamp c
61. re mat riel Les d tails sur ces circonstances figurent dans un encadr semblable celui ci Caution Attenzione Con il termine CAUTION ATTENZIONE vengono segnalate situazioni che potrebbero arrecare gravi danni allo strumento o ad altra apparecchiatura Troverete informazioni su tali circostanze in un riquadro come questo Caution Opgelet Betekent dat de genoemde handleiding nauwkeurig moet worden opgevolgd om beschadiging van het instrument te voorkomen Caution Aten o Significa que a instru o referida tem de ser respeitada para evitar a danifica o do aparelho Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com 25 Lambda 25 35 45 User s Guide We use the term WARNING to inform you about situations that could result in personal injury to yourself or other persons Details about these circumstances are in a box like this one WARNING CD Warning Warnung Bedeutet dap es bei Nichtbeachten der genannten Anweisung zu einer Verletzung des Benutzers kommen kann Warning Advarsel Betyder at brugeren kan blive kvestet hvis anvisningen ikke overholdes CED Warning Peligro Utilizamos el t rmino WARNING PELIGRO para informarle sobre situaciones que pueden provocar da os personales a usted o a otras personas En los recuadros como ste se proporciona informaci n sobre este tipo de circunstancias CF Warning Danger Nous
62. rescence Spectroscopy and Polarimetry To place an order request a free catalog or ask for information If you are located within the U S call toll free 1 800 762 402 8 a m to 8 p m EST Your order will be shipped promptly usually within 24 hours If you are located outside of the U S call your local PerkinElmer sales office Accessories spares and other parts and information are available on line at essentials perkinelmer com Quantity Part Number Thumbscrew for lamp mount B0119371 Sample Compartment Window B0098757 Qu o _ 104 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Analytical Notes Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com 1 Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Analytical Notes Unusual Samples If a sample is chemically stable and undergoes no physical or chemical change other than to absorb incident radiation errors in photometric values should not be caused by the sample Many samples are not stable and special consideration must be given to them Volatile Samples Some liquid samples are so volatile that their concentration can change while recording is in progress If this occurs the resulting data will lack reproducibility If you are analyzing volatile samples use stoppered cells to prevent this
63. t always be expected to have sufficient reliability or repeatability For samples of this type use a quantitative method that takes advantage of the change in transmittance with time at a fixed wavelength For details refer to reference books covering this specific subject Photoactive Samples Some samples are known to be photoactive in that they fluoresce upon absorbing radiation Since a small portion of the fluorescent radiation will be measured by the detector a higher apparent transmittance will often result Samples are also known that undergo photochemical reactions as they absorb radiation With such samples which are mostly biochemical lack of reproducibility will characterize the resultant data Other Sample Properties Samples that are polarizing in nature or have a double index of refraction are often difficult to measure accurately The emerging monochromatic radiation is slightly polarized due to having been refracted Thin film samples also pose a problem since optical interferences may develop causing a regular interference pattern to be superimposed on the spectral curve Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Analytical Notes Solvent Properties The solvent should meet the following requirements e It should dissolve the sample without reacting with it e The radiation absorption in the scanning region should be low High absorption by th
64. t in the lamp compartment is asymmetric the deuterium lamp connector can be inserted in one direction only Make certain that the connector is the right way round before inserting it Never attempt to insert the connector by force 9 10 11 Wipe the lamp window with a soft cloth moistened with alcohol to remove dirt since this would otherwise be burned in when the lamp is hot Replace the lamp baffle using the reverse of the procedure described in step 3 Replace the lamp compartment cover This completes the deuterium lamp replacement procedure Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Maintenance Lamp Energy Attenuator An attenuator is located in the lamp compartment between the deuterium lamp and the beam entrance slit If required you can decrease or increase the energy by placing the attenuator into or taking the attenuator out of the beam The attenuator is set at the factory normally in the out position Electrical Hazard Switch off the spectrometer and remove the plug from the electrical supply before starting with the replacement Risk of Burns WARNING If the old lamp was lighted allow it to cool before proceeding with the replacement UV Radiation The lamps emit intense radiation which can damage your eyes Do not open the lamp compartment when the lamps are on Do not gaze into a lighted lamp Operate the attenuator as follows
65. tassium dichromate solution Transmission lt 0 02 at 220 nm 340 nm and 370 nm Absorbance gt 2 measured at 200 nm with potassium chloride solution against distilled water Absorbance 0 001 1 nm slit corrected 200 nm to 1100 nm scan speed 240 nm min smooth 2 1 nm slit Absorbance lt 0 00008 RMS Absorbance lt 0 0003 peak to peak 3 min at absorbance 0 wavelength 500 nm and response 2 s Absorbance lt 0 0003 per hour 500 nm after warmup B K2C120 7 60 06 pg mL 0 5 in sulfuric acid c H SO4 0 005 mol L B KC1 12 mg mL 4 0 5 43 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Data Output Digital port One RS 232 C interface serial for connecting a PC 44 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com System Description Lambda 35 Specification General Type Scanning double beam spectrometer for the UV Vis range operation by PC Dimensions Width 650 nm Height 260 nm Depth 560 nm Ambient operating temperature 15 C to 35 C Humidity range 20 to 80 relative humidity without condensation Technical Standard In compliance with the requirements for technical instruments stipulated by IEC 61010 1 1990 and amendments A1 1992 and A2 1995 Radio interference suppression In compliance wit
66. terface serial for connecting a PC 52 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Installation 4 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com 1 Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Installation Preparing the Working Area For maximum stability and minimum maintenance observe the following requirements when siting the instrument e A firm base free from vibration e Enough space around and underneath the instrument for efficient air circulation e A constant temperature between 15 C and 35 C e Constant humidity between 20 and 80 relative humidity e Anatmosphere free from dust and corrosive fumes e Keep out of direct sunlight Illumination with diffuse lighting is ideal e A suitable source of electrical power should be located in the vicinity of the instrument Electrical power must be available at a proper earth grounded 3 wire electrical outlet e The standard sample compartment baseplates have drain holes in them to run off spilled liquids to the benchtop underneath the instrument If required a sheet of thick filter paper can be placed under the instrument 55 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide Front view
67. tific com System Description Introduction The Lambda 25 35 45 are versatile spectrometers operating in the ultraviolet UV and visible Vis spectral ranges The spectrometers feature a double beam all reflecting system Lambda 25 35 45 spectrometers are usable in a wide range of applications as indicated by their performance specification Features Sample compartment Ce BE Connector panel Figure 5 Lambda 25 35 45 Spectrometer Features 37 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 38 NOTE Optical System of the Lambda 25 35 45 The Lambda 25 35 45 UV Vis Spectrometers features an all reflecting optical system The optical components are coated with silica for durability A holographic grating is used in the monochromator The optical system is depicted schematically in Figure 6 The monochromator is a holographic concave grating with 1053 lines mm in the center Two radiation sources a deuterium lamp and a halogen lamp cover the working wavelength range of the spectrometer For operation in the visible Vis range mirror M1 reflects the radiation from the halogen lamp onto source mirror M2 At the same time M1 blocks the radiation from the deuterium lamp In the Lambda 45 M2 is substituted by a pre monochromator grating increasing the straylight performance of the instrument For operation in
68. w in UV WinLab Method will be displayed Accessories Samples can be applied either manually or with the help of a number of accessories The following accessories are currently available Changers 9 Cell Changer Peltier thermostatted 13 Cell Changer Linear Transporter With 8 cell holder With 5 cell long pathlength holder With 6 cell holder Gel plate reader continuous or random Vacuum Sipper or Peristaltic Sipper 82 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Accessories Requirements for Operation with Accessories The following preconditions must be fulfilled in order to operate with accessories e The accessory in use must be activated on the accessory page of the UV WinLab software e The connector panel for the accessory in question must be installed in the spectrometer Some accessories such as Peltier cell holder and 9 cell Peltier changer require an upgrade board fitted to the power supply unit 83 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Lambda 25 35 45 User s Guide 84 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com Maintenance Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com 1 Scientific Quality Instrumentation Guaranteed 888 88 SOU

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