SYBR Green qPCR Master Mix User Guide
Contents
1. 0 ee Li Ct Ct 4 40 72 C PAGE amp Tel 400 668 6834 Fax 021 68591981 Email order biotool cn2. amp lt biotoolcn Save time Save funding 2x SYBR Green qPCR Master MiIx a eS tek 2x SYBR Green 5 ml 25 ml 25 mlx2 qPCR Master 200 rxn 1000 rxn 2000 rxn 20kl 50hl MiXa 50Xx ROX Reference 200 Hl 1000 Hl 2000 ul Dye 1 2x SYBR Green qPCR pe 10 je Master Mix 1 100ng 5O0x ROX Reference 200 Hl 1000 Hl 2000 Hl Dye 2 5uM 02 36 50 00nM a DNA dNTPS Mg2 SYBR Green I dye 5 IE hs hm az 0 2 5 10HI 20 C 2 a Hot Start Real time PCR ROX Step DNA PCR Melt Curve Polymerase Bio Rad CFX96 CFX384 iCycleriQ Activation iQ 5 MyiQYOpticon Opticon 2 CYCLE 40 cycles Chromo4 MiniOpticon HOLD FT CYCLE 1 cycle Cepheid SmartCycler Denatur Extend Temp 950 C 950 C 600 C 950 C 950 C Reference realplex 2s lllumina Eco qPCR Dye Qiagen Corbett Rotor Gene Q Time en 15 secs be 15 secs 60secs 15secs Rotor Gene 3000 Rotor Gene 6000 er Bo El Roche Applied Science LightCycler 480 Thermo Scientific PikoReal Cycl
3. er RS 3 Applied Biosystems 5700 7000 7300 7700 1 2 3 Dye 1 7900 7900HT 7900HT Fast StepOne et StepOne Plus Ste DNA R Polymerase PCR Melt Curve Activation ee Applied Biosystems 7500 7500 Fast Dye 2 ViiA 7 Stratagene MX4000 MX3005P CYCLE 40 cycles MX3000P HOLD CYCLE 1 cyde Deretur el Temp 95 0 C 95 0 C 60 0 C 95 0 C 60 0 C 95 0 C Time a 3 secs Rs 15 secs 60secs 15 secs Volume 50 HL 50 HL 95 C 5 10min DNA GC 10min 10HI 20HI amp Tel 400 668 6834 Fax 021 68591981 Email order biotool cn amp lt biotoolLcn Save time Save funding _ J Ns ee 35 Le Ct yy
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