User Guide - Instrumart
Contents
1. 8256B PerpHecT ROSS combination pH electrode with epoxy body and BNC connector 8272B PerpHecT ROSS Sure Flow combination pH electrode with glass body and BNC connector e All electrodes with a waterproof BNC BNC and U S standard connector have one meter cable lengths e All electrodes with a screw cap connector require a separate cable e Electrodes with a waterproof BNC connector can be used on any pH meter with a BNC connection e The 800500U ROSS Ultra and 800300 ROSS reference half cells can be used with the 8101BNWP 8101SC ROSS pH half cell or 8411BN ROSS sodium half cell ROSS Electrode User Guide ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc Required Equipment 1 Thermo Scientific Orion pH meter such as the 3 Star pH meter 4 Star pH ISE meter 4 Star pH DO meter 4 Star pH conductivity meter or 5 Star pH ISE DO conductivity meter ROSS electrodes can be used on any pH meter with a BNC or U S standard connection The electrodes can also be used on meters with a variety of inputs when an adapter cable is used Visit www thermo com water for details 2 Thermo Scientific Orion ROSS Ultra ROSS ROSS Sure Flow or PerpHecT ROSS electrode 3 ROSS filling solution Cat No 810007 Do not use any filling solution that contains silver as silver will damage the electrode 4 ROSS pH electrode storage solution Cat No 810001 or standard pH electrode storage so2. BNC connector U S standard 8155SC connector screw cap connector 8162SC ROSS combination pH electrode with glass body 14 15 standard taper and screw cap connector 8163BNWP ROSS combination pH electrode with 8163SC glass body spear tip and waterproof BNC connector screw cap connector 2 ROSS Electrode User Guide ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc Cat No Description 8101BNWP ROSS pH half cell electrode with glass body 8101SC and waterproof BNC connector screw cap connector 800300 ROSS Sure Flow reference half cell electrode with glass body and pin tip connector 8165BNWP ROSS Sure Flow combination pH electrode 8165DN with epoxy body and waterproof BNC connector E DIN waterproof connector 8172BNWP ROSS Sure Flow combination pH electrode 8172DN with glass body and waterproof BNC connector E DIN waterproof connector 8175BNWP ROSS Sure Flow combination pH electrode with epoxy body semi micro tip and waterproof BNC connector 8202B PerpHecT ROSS combination pH electrode with glass body and BNC connector 8203B PerpHecT ROSS combination pH electrode with glass body semi micro tip and BNC connector 8220BNWP PerpHecT ROSS combination pH electrode with glass body micro tip and waterproof BNC connector 8235B PerpHecT ROSS combination pH electrode with epoxy body flat surface tip and BNC connector
3. Guide ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc Two Buffer Calibration This procedure is recommended for precise measurements 1 Select two buffers that bracket the expected sample pH The first buffer should be near the electrode isopotential point pH 7 and the second should be near the expected sample pH pH 4 or pH 10 2 The buffers should be at same temperature as the sample If the buffers and samples are at varying temperatures temperature compensation is recommended 3 Rinse the electrode first with distilled water and then with the first buffer 4 Place the electrode into the first buffer When the reading is stable set the meter to the pH value of the buffer at the measured temperature Refer to the meter user guide for a detailed procedure Table 1 provides pH values at various temperatures 5 Rinse the electrode first with distilled water and then with the second buffer 6 Place the electrode into the second buffer When the reading is stable set the meter to the pH value of the buffer at the measured temperature Refer to the meter user guide for a detailed procedure Table 1 provides pH values at various temperatures 7 Proceed to the pH Measurement section Temperature 40 50 60 70 80 90 1 69 1 71 1 72 1 74 1527 1 79 3 75 3 75 4 04 4 06 4 09 4 13 4 16 4 21 6 84 6 83 6 84 6 85 6 86 6 88 6 97 6 97 6 97 6 99 7 03 7 08 738 737 9 07 9 01 8 96 8 92
4. Inc Electrode Maintenance 1 Inspect the electrode for scratches cracks salt crystal buildup or membrane junction deposits Rinse off any salt buildup with distilled water Remove any membrane junction deposits as directed in the General Cleaning section Drain the reference chamber flush it with fresh ROSS filling solution and refill the chamber with fresh ROSS filling solution Electrode Cleaning Procedures General Cleaning 1 Soak the electrode in 0 1 M HCI or HNO for half an hour The electrode can also be soaked for 15 minutes in a 1 10 dilution of household laundry bleach or a 0 1 to 0 5 liquid detergent solution mixed with hot water The solution should be stirrer at a moderate to fast rate Drain the reference chamber and refill it with fresh ROSS filling solution Soak the electrode in ROSS pH electrode storage solution for at least one hour Cleaning Solutions Cat No 900021 pH cleaning solu contaminants ion A for removing protein Cat No 900022 pH clean contaminants Cat No 900023 pH clean Cat No 900024 pH clean grease contaminants Cat No 900020 pH clean solutions A B C and D ing so ing so ing so ing so ROSS Electrode User Guide ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc u u u u ion B for removing bacterial ion C for general cleaning ion D for removing oil and ion kit includes c
5. 001 or standard pH electrode storage solution Cat No 910001 for 30 to 60 minutes For enhanced electrode performance use ROSS pH electrode storage solution Connect the electrode to the meter filling hole filling ROSS solution internal chamber reference ceramic reference pH junction sensing bulb ROSS Combination pH Electrode ROSS Electrode User Guide ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc Electrode Preparation for Sure Flow Models 1 Remove the protective shipping cap or shipping sleeve from the sensing element and save the cap for storage 2 Clean any salt deposits from the exterior of the electrode by rinsing with distilled water 3 Uncover the filling hole by removing the tape and then add ROSS filling solution Cat No 810007 to the electrode To maintain an adequate flow rate the level of filling solution must always be above the reference junction and at least one inch above the sample level The filling hole should be open whenever the electrode is in use 4 Wet the junction by pressing down on the electrode cap to let a few drops of the ROSS filling solution flow out of the electrode and then replenish any lost solution 5 Shake the electrode downward similar to a clinical thermometer to remove air bubbles 6 Soak electrode in ROSS pH electrode storage solution Cat No 810001 or standard pH electrode storage solution Cat No 910001 for 30 to
6. 60 minutes For enhanced electrode performance use ROSS pH electrode storage solution 7 Connect the electrode to the meter Q Orion 8175BN i fill hole NIJ 2 iS Ss cS S 5 fit reference ROSS filling solution internal chamber reference Sure Flow pH Fi junction sensing bulb ROSS Sure Flow Combination pH Electrode ROSS Electrode User Guide 7 ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc Measuring Hints Always use fresh buffers for calibration Choose buffers that are one to three pH units apart Check the electrode slope daily by performing a two buffer calibration The slope should be 92 to 102 Except as noted in the Sample Requirements section only use ROSS filling solution Cat No 810007 Do not use any filling solution that may contain silver Remove the filling hole cover during measurements to ensure a uniform flow of filling solution Between measurements rinse electrodes with distilled water and then with the next solution to be measured Stir all buffers and samples at a uniform rate Place a piece of insulating material such as Styrofoam or cardboard between the magnetic stirrer and beaker to prevent measurement errors from the transfer of heat to the sample Since ROSS electrodes respond faster to temperature changes pH changes due to temperature variations will be noticed To reduce the chance of error due to polarization avoid rubbing
7. 8 89 8 85 9 89 9 83 ROSS Electrode User Guide 11 ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc pH Measurement 1 Calibrate the electrode as described in the Electrode Calibration section 2 Rinse the electrode with distilled water and then with the sample 3 Place the electrode into the sample 4 When the reading is stable record the pH and temperature of the sample Electrode Storage To ensure a quick response and free flowing junction the sensing element and reference junction must not dry out Short term Storage up to one week Soak the electrode in ROSS pH electrode storage solution Cat No 810001 or standard pH electrode storage solution Cat No 910001 For enhanced electrode performance use ROSS pH electrode storage solution To minimize the chance of breakage the 8220BNWP PerpHecT ROSS micro pH electrode should be attached to an electrode stand and suspended in a beaker that contains storage solution The electrode should not touch the sides or bottom of the beaker Long term Storage more than one week Fill the reference chamber and securely cover the filling hole Cover the sensing element and reference junction with the protective cap sleeve containing a few drops of storage solution Before returning the electrode to use prepare it as a new electrode 12 ROSS Electrode User Guide ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific
8. Drift can occur when the internal reference elements inside the pH and reference portions of the electrode are reaching thermal equilibrium after a temperature change Long term drift or slow response can last until the sample and electrode are at the same temperature ROSS electrodes use a patented internal reference to minimize the equilibration time 14 ROSS Electrode User Guide ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc Temperature Sensor Errors When a pH and temperature probe are placed into a sample that varies significantly in temperature the readings can drift for two reasons First the temperature response of the electrode and temperature probe may not be similar which prolongs equilibration and drift Second a sample may not have a uniform temperature Therefore the pH electrode and temperature probe are responding to different environments Using LogR technology PerpHecT meters sense the temperature directly from the PerpHecT ROSS pH electrodes The pH and temperature response is identical and both measurements occur at the sensing bulb Drift is minimized and errors due to environmental differences are eliminated Sure Flow Electrode Leak Rate The junction of the ROSS Sure Flow electrodes has been designed to leak at a rate of approximately 1 mL per 24 hours To keep the junction from clogging the leak rate is slightly higher than conventional electrodes During the average pH m
9. Series A meters and 900A printer are protected by U S patents 5 198 093 D334 208 and D346 753 ionplus electrodes and Optimum Results solutions are protected by US Patent 5 830 338 ROSS Ultra electrodes are protected by US patents 6 793 787 Orion ORP Standard is protected by US Patent 6 350 367 Orion NoCal electrodes with stabilized potential patent pending 2007 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries The specifications descriptions drawings ordering information and part numbers within this document are subject to change without notice This publication supersedes all previous publications on this subject Introduction This user guide contains information on the preparation operation and maintenance of the Thermo Scientific Orion ROSS Ultra ROSS ROSS Sure Flow and PerpHecT ROSS electrodes All ROSS electrodes have a pH range of 0 to 14 pH precision of 0 01 and temperature range of 0 to 100 C ROSS electrodes can be used in samples that contain TRIS sulfides and proteins since they do not contain silver or mercury ROSS electrodes incorporate the patented ROSS internal reference system that provides superior measurement stability faster response greater accuracy and more reproducible results than conventional electrodes ROSS electrodes provide readings stable to 0 01 pH in less than 30 seconds even in the extrem
10. User Guide ROSS Ultra ROSSE ROSSE Sure Flow and PerpHecT ROSSE Electrodes ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc AQUAfast Cahn ionplus KNIpHE No Cal ORION perpHect PerpHecT PerpHecTion pHISA pHuture Pure Water Sage Sensing the Future SensorLink ROSS ROSS Ultra Sure Flow Titrator PLUS and TURBO2 are registered trademarks of Thermo Fisher 1 888 pHAX ION A All in One Aplus AQUAsnap AssuredAccuracy AUTO BAR AUTO CAL AUTO DISPENSER Auto ID AUTO LOG AUTO READ AUTO STIR Auto Test BOD AutoEZ Cable Free CERTI CAL CISA DataCOLLECT DataPLUS digital LogR DirectCal DuraProbe Environmental Product Authority Extra Easy Extra Value FAST QC GAP GLPcal GLPcheck GLPdoc ISEasy KAP LabConnect LogR Low Maintenance Triode Minimum Stir Requirement MSR NISS One Touch One Touch Calibration One Touch Measurement Optimum Results Orion Star Pentrode pHuture MMS pHuture Pentrode pHuture Quatrode pHuture Triode Quatrode QuiKcheK rf link ROSS Resolution SAOB SMART AVERAGING Smart CheK SMART STABILITY Stacked Star Navigator 21 Stat Face The Enhanced Lab ThermaSense Triode TRIUMpH Unbreakable pH Universal Access are trademarks of Thermo Fisher Guaranteed Success and The Technical Edge are service marks of Thermo Fisher PerpHecT meters are protected by U S patent 6 168 707 PerpHecT ROSS are protected by U S patent 6 168 707 ORION
11. ations The accuracy values are valid only when the temperature calibration is performed within the stated temperature range The higher temperature range data will apply to measurements made above that temperature range provided that calibration points are within 20 C of each other When highly accurate pH results are desired a separate ATC probe is recommended One Point Temperature Calibration Average Average Average Average temp error temp error pH error pH error Electrode 20 30 C 30 50 C 20 30 C 30 50 C 8202BN 0 13 0 07 0 002 0 001 8203BN 0 24 0 17 0 003 0 002 8235BN 0 91 0 95 0 011 0 011 8256BN 0 09 0 10 0 001 0 001 Two Point Temperature Calibration Average Average Average Average temp error temp error pH error pH error Electrode 0 25 C 20 40 C 0 25 C 20 40 C 8202BN 0 36 0 10 0 004 0 001 8203BN 0 13 0 02 0 002 0 000 8235BN 0 04 0 14 0 000 0 002 8256BN 0 22 0 06 0 003 0 001 Three Point Temperature Calibration Average Average Average pH Average pH temp error temp error error error Electrode 0 25 C 20 40 C 0 25 C 20 40 C 8202BN 0 04 0 03 0 000 0 000 8203BN 0 04 0 01 0 000 0 000 8235BN 0 07 0 06 0 001 0 001 8256BN 0 04 0 06 0 000 0 001 16 ROSS Electrode User Guide ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc Interferences Sodium ions are the principal interference of the pH electrode and cause increasing error at higher pH lower hydrogen ion ac
12. c Inc Electrode Calibration General Calibration Procedure For detailed instructions on pH calibration manual pH calibration and temperature compensation consult your meter user guide When using PerpHecT ROSS electrodes with a PerpHecT pH meter refer to the PerpHecT meter user guide for instructions on temperature calibration and LogR temperature compensated pH measurements One Buffer Calibration 1 10 Choose a buffer near expected sample pH The buffer should be at same temperature as the sample If the buffer and samples are at varying temperatures temperature compensation is recommended Prepare the meter according to the meter user guide Rinse the electrode first with distilled water and then with the buffer being used for calibration Place the electrode into the buffer When the reading is stable set the meter to the pH value of the buffer at the measured temperature Refer to the meter user guide for a detailed procedure Table 1 provides pH values at various temperatures Proceed to the pH Measurement section Table 1 pH Values of Buffers at Various Temperatures Nominal Temperature Buffer Value EE eee at 25 C 0 D9 10 20 30 1 68 1 67 1 67 1 67 1 67 1 68 3 78 3 86 3 84 3 82 3 79 3 77 4 01 4 00 4 00 4 00 4 00 4 02 6 86 6 98 6 95 6 92 6 87 6 85 7 00 7 11 7 08 7 06 7 01 6 98 7 41 7 53 7 50 747 743 740 9 18 9 46 9 40 9 33 9 23 9 14 10 01 10 32 10 25 10 18 10 006 9 97 ROSS Electrode User
13. e 3 Rinse the electrode with distilled water and then insert the electrode into fresh pH 7 buffer 4 When the reading is stable record the mV value of the pH 7 buffer The mV value should be 30 to 30 mV 5 Rinse the electrode with distilled water and then insert the electrode into fresh pH 4 buffer 6 When the reading is stable record the mV value of the pH 4 buffer The mV value should be 150 to 210 mV 7 Calculate the absolute mV difference between the two buffers The mV difference should be 160 to 180 mV The actual mV values will change as the electrode ages but the mV difference between the two buffers should always be 160 to 180 mV If the electrode fails this procedure clean the electrode thoroughly as directed in the Electrode Maintenance section If the electrode response is slow or drifting drain and refill the electrode with fresh ROSS filling solution Cat No 810007 Replace the electrode if cleaning and maintenance fail to rejuvenate it 18 ROSS Electrode User Guide ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc Sample Application The electrode and meter may operate with buffers but not with the sample In this case check the sample composition for interferences incompatibilities or temperature effects Refer to the Interferences section Technique If trouble persists review operating procedures Review calibration and measurement sections to be sure pro
14. e case of samples varying from one another by 50 C or more Results are three to five times more precise than those obtained with conventional electrodes Electrode drift is less than 0 002 pH per day so restandardization is minimized ROSS electrodes have a double junction reference so the filling solution can be modified to more closely match the sample composition in high pH low pH or non aqueous solutions ROSS Ultra Electrodes ROSS Ultra electrodes offer the ultimate measurement stability and the least long term drift plus a two year warranty ROSS Electrodes ROSS electrodes provide precise pH measurements and measurement stability regardless of temperature changes or sample composition ROSS Sure Flow Electrodes ROSS Sure Flow electrodes incorporate a free flowing junction that is easy to clean and never clogs These electrodes are ideal for dirty or viscous samples that clog traditional electrodes PerpHecT ROSS Electrodes PerpHecT ROSS electrodes are designed to be used with PerpHecT meters to provide both pH and temperature measurements The PerpHecT meters use patented LogR technology that allows temperature to be measured using the resistance of the PerpHecT ROSS electrode ROSS Electrode User Guide 1 ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc Cat No Descrip
15. easurement time the loss of filling solution is minimal and the filling solution does not contaminate the sample PerpHecT ROSS Micro pH Electrode The 8220BNWP PerpHecT ROSS micro pH electrode has been designed to measure extremely small samples The measuring tip is 3 mm in diameter and 40 mm long The minimum depth of immersion is 4 5 mm and the minimum sample size is 15 uL in 384 well plates ROSS Electrode User Guide 15 ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc PerpHecT ROSS Electrode Operation with PerpHecT pH Meters When PerpHecT ROSS electrodes are used with a PerpHecT pH meter enhanced temperature compensation is achieved without the need of a separate ATC probe Using LogR technology the temperature of the solution is measured through the resistance of the pH electrode PerpHecT ROSS pH electrodes are manufactured to meet the PerpHecT meter specifications so optimum performance and accuracy are achieved in LogR mode Each PerpHecT ROSS pH electrode must be calibrated for temperature before a pH measurement is performed using LogR technology Refer to the PerpHecT meter user guide for details For maximum precision a three point temperature calibration is recommended Do not perform a one point temperature calibration if measured solutions will be below 20 C The following tables illustrate the expected pH compensation error for one two and three point temperature calibr
16. leaning 13 Electrode Characteristics Temperature Effects The most common cause of error in pH measurements is temperature There are at least five ways that temperature variations can affect pH electrode slope buffers samples reference element drift and temperature sensor errors Electrode Slope Changes The electrode slope will change with variations in temperature Slope changes may be compensated manually automatically with an automatic temperature compensation ATC probe or with LogR technology when using a PerpHecT meter and electrode Thermo Scientific Orion pH meters calculate the slope based on the measured temperature and automatically adjust the pH value based on the temperature Buffer and Sample pH Changes Buffer and sample pH values change with temperature because of their temperature dependent chemical equilibria The pH electrode should be calibrated with buffers that have known pH values at different temperatures Buffer values at different temperatures are given in Table 1 Thermo Scientific Orion pH meters automatically calibrate with the correct pH buffer values based on the measured temperature All pH meters are unable to correct pH values back to a reference temperature because every sample has a unique pH value vs temperature relationship Therefore calibration and measurements should be performed at the same temperature and pH values should be reported with temperature Reference Element Drift
17. lution Cat No 910001 For enhanced electrode performance use ROSS pH electrode storage solution 5 pH buffers at least two pH buffers are recommended for precise measurements One buffer should be near pH 7 and buffers should be one to three pH units apart 6 Beakers plastic or glass 7 Magnetic stirrer or Orion stirrer probe Cat No 096019 The stirrer probe can be used with 3 Star 4 Star and 5 Star benchtop meters 8 Distilled or deionized water 4 ROSS Electrode User Guide ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc Sample Requirements A benefit of the ROSS pH electrodes is that the filling solution composition may be changed depending on sample requirements The ROSS pH electrode filling solution Cat No 810007 is 3M KCl For solutions that precipitate in the presence of chloride ions the electrode can be filled with 10 KNOs Cat No 900003 Electrodes with an epoxy body should only be used in aqueous solutions Electrodes with a glass body may be used in non aqueous solutions and solutions that contain organic solvents A minimum of 20 water must be present in the sample for the best measurement results If there is a great deal of drift when using the ROSS electrode filled with ROSS filling solution try filling the electrode with a mixture of methanol and water saturated with KCI The ratio of methanol to water should be similar to the sample composition If u
18. or wiping the electrode bulb Use a lint free tissue and gently blot the electrode bulb If the electrode is used in dirty or viscous samples or the electrode response becomes sluggish empty the electrode completely and hold the junction under running water Empty any water from the electrode and refill it with fresh ROSS filling solution If a Sure Flow electrode is used in dirty or viscous samples or the electrode response becomes sluggish empty the electrode completely and hold the junction open under running water Empty any water from the electrode and refill it with fresh ROSS filling solution Press down on the electrode cap to let a few drops of the ROSS filling solution flow out of the electrode and then replenish any lost solution Flat surface ROSS electrodes may be used on any moist surface or in liquids ROSS Electrode User Guide ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc e Handle Orion 8220BNWP with care Do not touch the pH bulb and stem against the bottom or walls of the sample containers Filling hole should be uncovered Filling solution level must be higher than sample level Reference junction must be immersed Fresh pH buffer Place insulation between stirrer and beaker Stir all samples and buffers Measuring Hints Using a Flat Surface Electrode ROSS Electrode User Guide ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientifi
19. per technique has been followed Assistance After troubleshooting all components of your measurement system contact Technical Support Within the United States call 1 800 225 1480 and outside the United States call 978 232 6000 or fax 978 232 6031 In Europe the Middle East and Africa contact your local authorized dealer For the most current contact information visit www thermo com water Warranty For the most current warranty information visit www thermo com water ROSS Electrode User Guide 19 ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc Ordering Information Electrodes Refer to the Introduction section for a complete list of ROSS Ultra ROSS ROSS Sure Flow and PerpHecT ROSS electrodes Accessories Cat No Description 810007 ROSS filling solution 5 x 60 mL bottles 810001 ROSS pH electrode storage solution 475 mL bottle 910001 pH electrode storage solution 475 mL bottle 900020 pH cleaning solution kit includes 1 x 15 mL bottle each of cleaning solutions A B C and D pipette and beaker 900021 pH cleaning solution A includes 4 x 15 mL bottles pipette and beaker 900022 pH cleaning solution B includes 4 x 15 mL bottles pipette and beaker 900023 pH cleaning solution C includes 4 x 15 mL bottles pipette and beaker 900024 pH cleaning solution D includes 4 x 15 mL bottle
20. s pipette and beaker 910199 All in One pH buffer kit includes 475 mL bottle each of pH 4 01 7 00 and 10 01 buffers and pH electrode storage solution and pH electrode storage bottle 910168 pH 1 68 buffer 475 mL bottle 910104 pH 4 01 buffer 475 mL bottle 910105 pH 5 00 buffer 475 mL bottle 910686 pH 6 86 buffer 475 mL bottle 910107 pH 7 00 buffer 475 mL bottle 910918 pH 9 18 buffer 475 mL bottle 910110 pH 10 01 buffer 475 mL bottle 910112 pH 12 46 buffer 475 mL bottle Visit www thermo com water for additional buffers and buffer sizes 20 ROSS Electrode User Guide ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc Thermo Fisher Scientific Environmental Instruments Water Analysis Instruments 166 Cummings Center Beverly MA 01915 USA Tel 978 232 6000 Toll Free 800 225 1480 Dom Fax 978 232 6015 Int l Fax 978 232 6031 254786 001 Rev A www thermo com water
21. sing the 8220BNWP PerpHecT ROSS micro pH electrode great care must be taken to thoroughly rinse out the reference chamber and junction before changing the reference solutions ROSS Electrode User Guide 5 ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc Electrode Preparation Electrode Preparation for Non Sure Flow Models 1 Remove the protective shipping cap or shipping sleeve from the sensing element and save the cap for storage Clean any salt deposits from the exterior of the electrode by rinsing with distilled water Handle the 8220BNWP PerpHecT ROSS micro pH electrode with great care Uncover the filling hole by removing the tape and then add ROSS filling solution Cat No 810007 to the electrode To maintain an adequate flow rate the level of filling solution must always be above the reference junction and at least one inch above the sample level The filling hole should be open whenever the electrode is in use Place the electrode in an electrode holder and suspend the electrode in air for 5 minutes to thoroughly wet the reference junction The 8220BNWP electrode does not need to be suspended in air Shake the electrode downward similar to a clinical thermometer to remove air bubbles Do not shake the 8220BNWP electrode unless there are air bubbles in the filling solution and then shake gently and with great care Soak electrode in ROSS pH electrode storage solution Cat No 810
22. tion 8102BNUWP ROSS Ultra combination pH electrode with glass body and waterproof BNC connector 8103BNUWP ROSS Ultra combination pH electrode with glass body semi micro tip and waterproof BNC connector 8104BNUWP ROSS Ultra combination pH electrode with glass body rugged bulb and waterproof BNC connector 8115BNUWP ROSS Ultra combination pH electrode with epoxy body semi micro tip and waterproof BNC connector 8135BNUWP ROSS Ultra combination pH electrode with epoxy body flat surface tip and waterproof BNC connector 8156BNUWP ROSS Ultra combination pH electrode with epoxy body and waterproof BNC connector 800500U ROSS Ultra reference half cell electrode with glass body and pin tip connector 8102BN ROSS combination pH electrode with glass 810200 body and BNC connector U S standard 8102SC connector screw cap connector 8103BN ROSS combination pH electrode with glass 810300 body semi micro tip and BNC connector 8103SC U S standard connector screw cap connector 8104BN ROSS combination pH electrode with glass 810400 body rugged bulb and BNC connector U S 8104SC standard connector screw cap connector 8115BN ROSS combination pH electrode with epoxy 8115SC body semi micro tip and BNC connector screw cap connector 8135BN ROSS combination pH electrode with epoxy 8135SC body flat surface tip and BNC connector screw cap connector 815600 ROSS combination pH electrode with epoxy 815500 body and
23. tivities and at higher temperatures Because the pH membrane is composed of special low sodium error glass error due to sodium is negligible when measuring at pH values less than 12 When measuring at pH values greater than 12 add the correction value from the nomograph below to the observed pH reading Sample pH reading pH correction to be added C 20 0 1M 25 30 0 005 sample 13 40 sodium oi 0 01 50 0 02 JA 0 03 12 y 20 C oos 0 05 80 25 0 06 0 1M se 008 0 10 40 11 0 15 50 0 20 0 25 60 0 30 0 40 70 0 50 0 60 10 0 70 80 5M Typical Sodium Error Example pH reading 12 10 Sodium concentration 0 5M Temperature 50 C Correction 0 01 Corrected pH reading 12 11 ROSS Electrode User Guide 17 ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc Troubleshooting Follow a systematic procedure to isolate the problem The pH measuring system can be divided into four components for ease in troubleshooting pH meter electrode sample application and technique pH Meter The meter is the easiest component to eliminate as a possible cause of error Thermo Scientific Orion pH meters include an instrument checkout procedure and shorting cap for convenience in troubleshooting Consult the pH meter user guide for directions Electrode To test electrode operation 1 Connect the electrode to a working meter that has a mV measuring mode 2 Set the meter to the mV measuring mod
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