1. a oN Molecular Cloning Laboratories User Manual Version 1 0 Revision Date 08 30 2013 Product name DnaK HSP70 E Coli Recombinant Cat DNAK 100 DNAK 200 DNAK 300 Description Dnak originally identified for its DNA replication by bacteriophage in E coli is the bacterial hsp70 chap erone This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress denatured proteins kDa 99 68 24 33 Dnak photo page Concentration 1 2 mg ml 1 1 Loading Buffer Sample Application Highest transformation efficiency General cloning Blue white selection Plasmid isolation Recommended Storage Conditions This product should be stored at 80 C Thaw on ice only before use Do not re freeze Genotype F 80dlacZ M15 lacZYA argF U169 recAl endAthsdR17 rk mk phoAsupE44 thi 1 gyrA96 relA1 Reference Woodcock D M Crowther D M Doherty J Jefferson S DeCruz E Noyer Weidner M Smith S S Mi chael M Z and Graham M W Nucl Acids Res 1989 17 3469 3478 1 650 872 0245 www mclab com 1