LineGene 9600 Clinical Operational Software（20，40，60，80）
Contents
1. a a a a a El 116341 TW 2 JU C 1 TU C C C C CT CA V C a laala 1 are anc 1 aTaYa a a WA CAM O C CATO 52 C C O 52 I C 11 a f Y a G de a a laala aia E LA VA LA LA A hd LA VA TA HA LA DAY E a a E a a Carr a JU To C ETC O OO CT a a 00000606 Left Module Temp C Right Module Temp fs EN Apply A F3 F4 Fs FB 10 1 Best Gain 2 a a a a a aaa 1116 3021 a a CU AC w ww 7 C v CATO C w III CAM DU v a a a ind a am wA C CATO wA J C C 7 CU C ES TAIATA a CON a a v J TO v C v v C C w v CATO DW 40 LineGene9600 Absolute Quantification a QC n a CM Aralia Lone a MI v C Y Di U v C C IZ v CA WV C C IZ w a a a atan alallala a a O C2. a Ma i LA A LA LA LA A LA Y LA A NA LA el Qul V LA V A LA LA A y aYa aln a n arala LA ww v A VAT A V V V LA 45 aldallala OY dafina wow LA hd LA LA uw el A hd w v Mt M LA V A V LA v el V A Y LA LineGene9600 Absolute Quantification ce Fluorescen BAMA AA AA O NN gre a a en C Y X C Fitted Data Fj Raw Data R Grad Temp Calculation C Select Block Scan Method M Select System ParametersiS Y Axis Calibration Y Baseline Measurement B Fluorescence Calibration D 51 LineGene9600 Absolute Quantification 2 Fluorescence Calibration Cycle Start Fluorescence Calibration Cancel C D ry 4 atan a C C TO 1 P C C C v C y 3 2 ry 4 a v v JU a P gt que 2 J NA an MA O nina NA C N an nina a Mate a mate NACA NO adm man
3. 2 AM ATAN 3 7 VM MC 5 d gt VJ M NM Mt u u a a 2 a ee LU LE Without the baseline parameters the Fluorescence data will not calibrated Continue da a atan D ry 4 LJ a nan a CAN CAT a 816814 E v INT LA M Y LA LA ees VA LA LA ww v LA VS LA a a aja car a a if Ad Y a h_ 44 LineGene9600 Absolute Quantification Look in G 1 Fae Files of type Crosstalk Para Files 2 Cancel Files of type Crosstalk Para Files m Cancel Gain600001 Files of type Gain Para Files Im 45 LineGene9600 Absolute Quantification ee CY TA mi a an a a QU D a a AY 2 22 a ee ang D Ma a D 27 men har E NO aa OA 8 Q an a a ala a a ALNA Y 2k 2K 2K ok ok a 1 men 7313 2 a 9 U y e y 9 y U y U VAT pa Files of type Cal Para Files m Cancel aa ay un L7 L4 NO
4. 52 LineGene9600 Absolute Quantification adaition Oou wantd4o use these function ou must enter password assword has Deen se 2 7 7 la Inn a hi tam anTala LA LA ww LA ww J TA v LA LA LA A LA LA y 3 LA v LA A LA Jnning 4An agaition Oou want to use th Jnction you must enter passworda Password na 7 7 2 2 are OH i aw a a alanEs HH 7 7 alibrate baseline automatica vill be saved automatica nfile baseline 696 gt Jnaer 1nstati directa eiecting substra pasenne omy 2 out_order_ot _cutime 7 Qtherwise om wat canceling background 18 om can be usec nstrumen 2 Jnnming 4An adaition ou Awant to use th Jnction you must enter passworda Password na 7 7 7 Insert Cal Para Export Raw Data and Export Cal Para are used to deal with data 7 company and user need notuse these function Analysis Options A Data Export E Capture Curv m Fitted Data F Raw DatafR Grad Temp Calculation C Gain Add Customized Dyes Select Block Scan Method M Select System Parameters sS Y Axis Calibration Y Baseline Measurement B Fluorescence Calibration D Best Gain Measurement G Best Gain Dyes Measured M Crosstalk Measurement R Crosstalk Dyes Me
5. NIA3M HOOG N3dO 402901 NOW f 401044 N3S WOO 9 ser _ S ul WOO e ser 10281 3ivadn 2 WOO 1 81 L SEr OO WOO e vzr WOO _ L vzr gaar _ WOO 1 22 4729344 d c944 112 Ler d 9144 DILAO 2 mE NIA Av 478934 SI SO SL vf EL OL vr aivadn 6 vr AS 9 pr AS vf _9 WOO S y vr 1i pr alvadn cef axl 2 Lor 0000071078050 Ag ldod AH NIA Ave Le 0000072078069 AY 64594 88 Note Note Note Note Note Note Note Note Note
6. 8000 00 6000 00 4 4000 00 L Fluorescence 2000 00 L 4 10 55 2616 00 Dye Group 1 24 50 0 00 Look in Deskop EF E Documents X Computer Network Places New Folder Please input password cancel Files of type Customized Para Files v _ Cancel 2 5 47 LineGene9600 Absolute Quantification nputand confirm password Caution password ove digit anc OK to popup Please input password Confirm password Save in Desktop de i E My Documents My Network Places New Folder Save as type Customized Para Files m Cad a a aim a a a alarm a AS E OPPO AO O 2o ueorescepce opmensg cha nns aTaYa 11 111 1 a in a YO ENT a LA Vso LA 5 LA v A LA LA LA LA v AO CcIem occucs ooeaseep oanunec oaaernaereye apc nQe ewmers sc OST te gt He on Same gt E oweitation Bas bu CAJA fh mmm emm Leper m 10 400 FX 8245 ta 4 7 A Ce ew s Amr emn MOR corpo Ada ra 48 Flo gt 1525 1504 1612 1
7. a 1 LA LA LA LA A A LA LA A A LA w VA ww LA A VA LA A t 7 t 3 t 0 7 A a a a n n aire LA LA LA A LA LA LA LA LA A LA A LA VA WI LA A aia E a a a a a a LA w VA LA ww LA LA LA A LA LA A t 7 fyi ria a a a t 7 LUL 1 Aire a a a a LLULL Ga aire Are a el ww LA w v LA uw hd A A v ww t 3 LELEL Ga alla ala ava w LA w LA A LA NA LA A esf VA A LA w LA A 3 t 7 5 t 7 3 a LULO Ga a a LA el LA A LA w LA LA Aj LA LA LA w LA LA LA w LA 5 35 Absolute Quantification LineGene9600 Y ww wp wr uw EE Se 36 LineGene9600 Absolute Quantificatio
8. Target Time Rate 2nd Temp Step Size Step Delay Grad Temp Sample Mode 00 00 10 60 0 00 00 00 4 0 None vl 30 0 00 00 00 4 0 None vl Cycles add 2 Delete Wee ala AIO na aldaYa QONmAan Mada A LA M v LAM KJ LA v w VA LA VA LA LA VA 113 5 113 5 ES Amn CY A AA nQrec M mania A arar Y LA LA LA v LA LA VA A JE J VA A LA Cycles add 3 O delete 4 405 Time H dio od Absolute Quantification LineGene9600 00 00 01 8 gt 30 0 00 00 01 4 0 Single Ternperature C Target 00 00 10 00 00 00 30 0 00 00 00 4 0 LineGene9600 Absolute Quantification a a a LY Avr a v w w TOUT C 3 DIU C w LA C C a a a atantaYa atantaYa a AU O v To C C w CATO a ala atan Avr Mn v JU ID w w C v C Tie C v w I V7 KJ LAM ky Cycles 99 lay Cycles 99 i 600 00 asc 00 00 00 00 00 10 31 LineGene9600 Target a ALCO ALN v v gt v DIN DIN a AQ MOTO SE
9. 831V3H ely ely Qn 9 NO MOTI3A xXovi8 ENO 3LIHM C ENO 3LIHM anid L ENO ang 349 Z LNO L LNO IL ZL ZNO ALL LL ZNO L4JOGOV 0 2 2 11009V 6 ZN9 8 2N9 89 2 9 ZNO OMV_S ZNO ORIGIN 614 2 S1d L ZNO 0 72deg 25 12500 0 0288deg DATA1 DATA2 8 RUN STOP CURRENT SET S NO das 30198 L EN9 FUC DIPSW 1P OFF 2P 3P 4P 0N 9 MOTI3A NO MOTI3A t NO H9V14 3LIHM C ENO 3LIHM anid L NO ania 349 LNZIAND dOLS LNFIAND NAAI H9014 00000060 8050 89d Z LNO L LNO LLON OOOO Z LNO L LNO WIL_ZL NO LL ZNO LHJOQOV _ ddOGOV 6 4919141 59 89 9 _ y 40 044 1026 91 T JL 440 8 814 _ NO ZMS L ZNO d8 O y UY y 1 440 5 a 1 IM EL 401044 6 d8 dSid 8zr
10. NT a witha di LA LA I LA I AI LA ww LA LA 3 v 1 TaYa 1 1 Ll EJ lo AY Y a with LA LA LA LA LA A y M LA wld LA LA LA LA LA 2 22 Romeve Programe 51 gs ed 1 eat bre tana re Qn o 5 UneGane aran be In m ata E To verrez Fez ge zur JON De emen e tuo Maru oft olt dues 2311 on Ira GARA TIA Add or Remove Programs w chee A 233 2 4 D 2741 prey tv GUN vrbe Se Prost 42 2 A gt Dr a a lt a PAT ANY aa a aja Y gt LA LA v 2 2 LA M A VA ww w LJ VA LA LA LA VA VAW Ninan ina nreac ile de Lan CAY LA A w VA LA LA 5 VA gt LA ww LA LA LA gt LA LA LA VLA ww LA LA anf rm aja ara aja a ile 1 6 41 6 41 6 3 CAY i LA v v LA 7 ANS v LA J LA LA LA LA v LA LA LineGene9600 Start Chapter 3 Start Caution Before initial use of this instrument correctly take out the
11. aw cal Ca 8 A alan aw Het 2 hen saving detection program le ne detau aving folde user name and the awing te netest name case of ne usernameorest name the cetautsavingiolde Save As dddddddddddddddd FFFFFFFFFFFFFFFsssss ee File Name 409 48 02 05 20 2009 Cancel C E de ntifi Lan a ime D D Lan LA A LA LA LA v LA hu LA v 21 lt lt LA v v VS LA AN y unhningand detection result of the detection program the entire detection em con LJ ren a MIO MALO Setup Program his part main pts up the detection orogram program section and 24 LineGene9600 Absolute Quantification Otley Mode i203 a oc 25 LineGene9600 26 Absolute Quantification LineGene9600 Absolute Quantification Liquid Quant ul 27 LineGene9600 Absolute Quantification SN Programs Notes SN Programs Notes rer LITE a 2 MoveDown D Remove R EE Y Copy C Paste P End End 28 LineGene9600 Absolute Quantification
12. 20 4 2 Sa 5 zh 2 gt 95 hat usc LineGene9600 Absolute Quantification 10000 00 T P j PE E t 1 7 VA 7 6000 00 fife a 2000 00 Y 114 1224 0 5 10 15 20 25 30 35 40 10000 00 p B4 33 00 10614 00 8000 00 HEX n Start 19 56 6000 00 ES E fy 7 gt 0 5 10 15 20 25 30 35 40 experiment Prepare for the reaction Run the experiment Experiment analysis Experiment report 3 Run the Experiment This section describes how to run operate the experiment after loadin the reaction plate and covers the operating of florescence curve the operating of temperature curve and prog Data export 49 End LineGene9600 Absolute Quantification 5 ARE d CO y Start system Ut or ore running program e module De 5 e green run _ Switch snail Il on_an e system us sena lOCKIAQ SOUNA alarm IS not on ana ea running State whose sampe software will show prompt and the alarm lamp will light on mode is none and At this case temperature program can run but the ihe creagentxelume fluorescence scanning data would become invalid
13. 34 Z CUSTOMIZE COLUMN iuto Rs nine 35 D COLUMN SELECTION i mtu M NEM 36 COLUMN EIBRAR Y 36 7 INSTRUMENT CALIBRATION 8 44 00004410 sss aset et 37 8 MEASURE CROSSTALK CALIBRATION 5 37 CROSSTALK GAIN PARAMETER MEASUREMENT sanierte 38 LOS YSTEMM INTENANCE ee seen 39 UPGRADING sn ee 39 12 CONVERT OLD FIRE INTO NEW EE 40 CHAPTER 6 OTHER FUNCTIONS irent aan 41 I ANSTRUMENT OPERATION Gera ee a enema 4 Id onec tns tune i 5022 tuu 4 A E 41 13 Ans ea dt ide 41 22021100 22 12 41 OPEN ANDES AVE FIE NA E 42 ee ee 42 DNO Tier 42 CHAPTER 7 MAINTENANCE 4 2 22 eei i ra dae A DU br 43 REGULAR CLEANING ESS LE LI 43 2 ANALYSIS AND ELIMINATION OF TROUBLES aan eek 43 11 LineGene9600 General Description Chapter 1 General description This chapter mainly describes the applications features specification model performance parameters and software fun
14. 89 1 gt 2 meting L Movebownt D Remove R 33 LineGene9600 Absolute Quantification e See ace h setting uo range 0 1 40 Target Rate SOAK 4 0 Target Rate 8 0 4 0 34 LineGene9600 Absolute Quantification 2 Untitled fqdper ARTES File F Sample Data S System Parameters P Analysis Options amp Data Capture Curves C Help H E 0738000 Run Programs Quantitative Analysis Melting Curve Analysis Data Query Setup Programs Prop 5 Unk Pos Blk 1 2 3 H 5 6 7 8 9 10 11 12 TevR ed ose rade Group No Concentration Note that all channels are allowed with LineGene 9680 80001 aja a aja a a aire aja A LA A w LA LA LA P el LA LA A LA A LA w a aire dh hd LA hd v A ww LA el el LA WA Ql V V A VA t 3 a a ala NN C a a NO C C AILIO a alfa ON a LA w A LA LA LA A LA LA A el t 7
15. Thermal sensitive fuse is damaged Loose plug pieces The hot lid is Contact the supplier or 11 Heating elements of hot lid is impossible to heat manufacturer damaged Eliminating contamination Each instrument shall Under no test tube state the Temperature sensor of hot lid 5 damaged correspond to baseline96 The test tube well or hot lid is document contaminated or After perennial use offset baseline b96 background would occur in the optical fluorescence value difference between wells increases or the parameters are mis used elements In this case contact background value 15 the manufacturer to very high re calibrate the background value There is the matter with quality To select applicable Reagent evaporation of test tube whose sealing is consumptive material which not tight has more tight capability You can measure by using Signal crosstalk Dye signal among channels ee Crosstalk Measurement and among channels exists crosstalk objectively save parameters to modify 84 LineGene9600 Maintenance Fluorescence detection value Irradiation by external strong Close external light source light During running of program the Close hot lid detection result hot lid is opened unreliable abnormal The photo electric system is Contact the supplier or damaged manufacturer Caution During the warranty period arbit
16. caused BIOHAZARD biohazard during use PROTECTIVE Protective earth is near to the place pasted EARTH this mark in instrument e Warning mark HOT SURFACE Warning The instrument is adhered with mark SURFACE During operation of instrument or a period immediately after completion of running of a program any portion of body shall not be contacted with metallic portion module nearby the said mark otherwise skin would be burnt Warning Operator may touch matter which is baneful to organism or is contagious during using instrument Operate should realize the harmfulness and operate in the condition which must accord with rules of PCR lab And operator must be IH LineGene9600 Important Notes trained or be provide with qualification j Sign of external packing _ Correct position of transport package is vertical upwards Be treated with care when transportation Breakables there is breakables in transport package The limit of stacking layer Transport package 15 not rained Maximum stacking layer of the same package is 2 24 m Temperature limit that transport package Temperature limit should keep is form 20C to 55C 3 EMC Consideration Note This is a class equipment only suitable for use in establishments other than domestic and those directly connected to a low voltage power supply network which supplies buildings used for domestic purpose 4 Maintenance
17. power plug from the power socket contact the supplier or ask qualified repair personnel for treatment e Liquid drops into inside of the instrument e he instrument is rained or watered e instrument works abnormally especially generates abnormal sound or odor Il LineGene9600 Important Notes e he instrument is dropped or its casing is damaged e The function of instrument obviously changes Caution When you deal with potential contagious matter such as flesh sample or reagent which is likely to touch skin protecting glove or other protecting measures are need to be used h Transportation again If transporting the instrument again the instrument and its detection wells need be cleared wholely before transportation and be disinfected by UV light After clearing the fixing pin shall be tightened to transportation well when the instrument stops running and power off so that motion componet of the instrumnt interior can be fixed and motion componet is avoided to be demaged during transportation Introduce of the location from transportation well is seen in chapter of fixing pin backout i Warning Signmark e Warning identification Place pasted this mark in instrument is DANGER danger if the instrument is used irrelevancy Place pasted this mark in instrument causes SCALDING high temperature and is scalding during use Place pasted this mark in instrument
18. 3 t BD a uv VV an nn alo a MIQ uraman ara DU Baseline Measurement B Fluorescence Calibration D Best Gain Measurement G Best Gain Dyes MeasurediM Crosstalk Measurement R Crosstalk Dyes MeasurediT oinput dye and _suitable qain for channel in the following dialogbox and select Measure en 7 7 Dye of Ch 1 Gain of Ch 1 ET Dye of Ch 2 Gain of Ch 2 Cance C Dye of Ch 3 MM Gain of Ch 3 Dye of Ch 4 Gain of Ch 4 DyeofchS E Gamofchs Dye of Ch 6 E Gain of Ch 6 Dye of Ch 7 D Gain of Ch 7 Dye of 8 Gain of Ch 8 E Measure Crosstalk Coefficient After running 5 1n meant numbe Orrespondence a n interface of run pop up the uppe ndow afterrun user ms ale Cancel to aqaut measure and mayresumethe upper produce to measure Againtinorder te assure correct 4 equired to measure o User can see dye in Crossta nnm a a a a a a a a 3ution 2 6 5 Ot Donc HERVE 2 cat 5 spectrum overrap eye pe oon cnanne and channe UCh ara aara eratara a a EE ey GEE 2 251 L Hd a MA a Ima ara
19. ACAM A Y LC ACAM A O NM NO Y LA VA LA Y v LA LA LA E UA LA v hd un 33 a ca NO ACAM AO v v LA LA LA w VA ICA TAS U 2 2 w hd J LA A LAI LA LA O MALO AAT NO O a a MALO a a a nfarm a I CS ANA NO v 3 v LA v w v w Au wel v LA LA MM LA LA LA LA INA v LA hi DI Vel O QM YT Xn IY a AO MIN O a I CS Ci T LA NICA LA LA hd LA hd v I LA v LA LA LA LA v LA J O S A O a A man a NILO a CAI a A AO A CAI 63 LA VA LA ICA LA hd 2 LA v LA hi LA LA t 5 t 7 a an Ad AM MATO Aman a Y 7 v v LA LA LA w v LAI LA was ANS LA v 11 M 11 6699 6655 O a I CS NUNAN a AO ala ANO a Na A Y v LA 7 5 v LA NA LA v LA v LA 11 6655 6637 O AI a AO ala ANO a CIA IY TO NO LA ILA v LA NA LA VA LA hd LA LA INA 46 LineGene9600 Absolute Quantification T 5 5 ElTips For Curves Cancel Alarm Alarm duration s 10000 00
20. Dimensions 430 395 352 mm Weight 28kg Caution F7 and F8 are the preserved dummy channels and may be customized as per actual requirements of customers 5 General description of functions of the necessary software a Parameters setting up function including temperature time cycles heating cooling rate selection of detection channel and yield of photo electric amplification tube b Note function of text contents c Sample material record function sample No sample name and sample data d Document running display function PCR heat cycle data display fluorescence detection data display and real time display of each data during running of instrument e Detection data analysis function The analysis function may be independently used under no connection of instrument f Analysis result output function It may output the analysis result to other type document e g EXCEL TXT document it can inquiry and print out analysis result modify printing format and select printing items g Document storage function setting up data running data and analysis result h Trouble protection and alarm function Caution The above mentioned software function is merely for reference The software function would be modified without notice LineGene9600 Preparations Chapter 2 Preparations This chapter mainly describes use transport and storage condition structural composition removal of fixing pin installation unloadin
21. VIS v t 3 ala a a w LA A LA A Start temperature 60 C Absolute Quantification Start Temp Time Step Sample Mode 00 00 00 C a a ara a m a CON FAG AV V 2 v w waw WTO v a an nn 4 v w VAT v v C w v CU a alallara E a a a v v VAT v v C C v Step temperature 0 5 Sampling m a ala a zar v DU gt gt IT OT C C 1 IT OT C C 5 17 5 ala 7 E V V C v SIT Iu C COYOTE OGC v w C C 5 17 5 aYalaaTala E 4 COLL a w w 2I V7 V v SIT Iu ZI VV 32 LineGene9600 Absolute Quantification oO Jo yum Hoi est RD nn ae a mt i 34 4 1 min eed cds Lem m BC Programs Notes D Eccer 0 Mkn ALE SN Programs Notes 1
22. d Clear all query conditions Query Result File Name Sample Id Sample Name Test Item Name Sex Age Case No Outpatient No Bed No Hospital Nationality Sampling Time Diagnosis Doctor Dept Test Result 1 3 Open and Save File e Click File Open the Open File dialog box will pop up The user can open experiment file fqd experiment template file fqt experiment filing file fqh and quantitative experiment file fqj in old version e Save file Click File Save Save As Save As Template 4 System Help Click Help Help Topics 5 About Click Help gt About gt the following window will pop up About Linecene9600 PR LineGene9600 version 1 0 Copyright Bioer 2011 80 LineGene9600 Maintenance Chapter 7 Maintenance 1 Regular cleaning In order to ensure normal operation detection and use the instrument shall be regularly cleaned e To clean of outer surface It shall be cleaned with soft cloth and if necessary soft cloth may be soaked with alcohol distilled water or cleaning paste e clean of module well It may be cleaned with nail wipes which does not bring dust accompanied brush and clean seft cleth and if necessary they may be soaked with 95 percentadittle of absolute ethyl alcohol used in medicine aleeheLor distilled water Warning 1 Before cleaning this instrument the power supply shall be cut off 2 At cleaning conic
23. files d Upgrade Upgrade faj fas file Upgrading Files Add uparading Files Remove selected Files Output directory 78 LineGene9600 Other Functions Chapter 6 Other Functions 1 Instrument Operation Instrument operations include connecting the instrument disconnecting the instrument and instrument information 1 1 Connect Instrument Click Instrument gt Connect select port number or select automatic port matching Connecting to instruzent mac ma Connecting to instruzent Flease wait When the instrument is connected the icon on the status bar will be if the instrument is disconnected the icon on the status bar will 4 1 2 Disconnect Click Instrument Disconnect disconnect currently connected instrument 1 3 Instrument Information When the instrument is connected the user can check the instrument information Click Instrument gt Instrument Information the following dialog box will pop up Instrument information includes instrument serial number runtime currently connected ports whether the experiment is in operation Instrument Information 600187 lihoursi3Minutes C na 2 2 Data Query Data Query is used to query the data already exported to the database Click Data Summary Data Query the following window will pop up The user can a Select database files 79 LineGene9600 Other Functions b Set up query condition c Query
24. of instrument The stained surface of this instrument if any shall be cleaned with soft cloth soaked with cleaning paste Heat conductive oil medium shall not be used in module holes of this instrument Module should be pushed in in time to prevent dust during normal conservation or after use Warning At cleaning of instrument its power supply shall be cut off e The instrument surface shall not be cleaned with corrosive cleaning agent e Instrument module includes precise optics dust foreign matter and residue should be avoided 5 After sales services The warranty content and scope are shown in the warranty sheet LineGene9600 Important Notes Note gt After unpacking immediately check the goods against the packing list In case of damaged or missed goods please immediately contact the supplier e After qualification of acceptance properly fill the product acceptance sheet and send or fax the copied sheet to the supplier for filing and maintenance e Before use of the product the user shall feedback the information registration table to Hangzhou Bioer Technology Co Ltd and obtain correct operation password e After unpacking the packing box and packing materials shall be well kept for future repair e The instrument should be disinfected before sent to the repair department e Fixing pin must be fixed correctly as manual before transporting instrument e Maintainer must disinfect the instrum
25. the current stage b Plus cycle 55 LineGene9600 Absolute Quantification c Minus cycle d Stop run Run Status Skip Current Stage Serial No 600187 Plus Cycle Minus Cycle e Plot display setting a Assay item b Plot color Plot Settings Assay Item Color 3 3 Monitor Operation e Monitor florescence curve click Florescence Curve Fluorescence o Curve Temperature Curve 2 Run Status Skip Current Stage 600187 Start Time 2011 12 16 09 22 42 Total Time 0 58 28 Leave Time Stage Hotlid Temp Cycle Target Temp Minus Cycle Stop Run End Time Segment Actual Temp Plus Cycle Run Status Running Fluorescence Curve Plot Settings Assay Item Color mm B Plate Setup oom Zoom Out Fluorescence 16 20 24 Sampling Count e Monitor temperature curve click Temperature Curve 56 LineGene9600 Absolute Quantification Hotlid Temp 7 Block Temp Temperature 00 25 00 00 26 40 00 28 20 00 30 00 00 31 40 Time 3 4 Program Setting The user can only check the program setting but cannot make modification Working state indication lamps on instrument The panel at upper left of instrument is fixed with 3 state indication lamps and they are related with system state during running of program e Running The indication lamp lights green which denotes that the entire machine is under program running state Alarm The indication lam
26. 0 72 74 76 78 80 82 84 86 88 90 92 94 96 Temperature e Check the reaction plate a Select relation plate hole site and check corresponding hole site curve x The default is all hole sites are selected b Zoom in zoom out and reset the reaction plate c Check well table information 64 LineGene9600 Absolute Quantification 57 Well Table e Setup assay a Set up assay b Set up color Melting Curve LEE target 1 v Color v 4 2 Adjust Parameter Reanalysis e Click Analysis Settings the Analysis Settings dialog box will pop up a Adjust the start cycle and end cycle of the baseline b Adjust Ct analysis algorithm c Set up the use of S fitting d Set up the stage to use for Ct analysis e Set up the automatic threshold value f Advanced setting g Standard curve setting 65 LineGene9600 Absolute Quantification Analysis Settings 4 Settings Advanced Settings Standard Curve Settings S Fitting Assay Item Threshold Start Cycle End Cycle target STER targetl SYBR Auto Auto Auto Y tuto Threshold Threshold 293 41 Auto Daseiine Start Cycle 3 End Cycle Apply Analysis Settings 66 LineGene9600 Absolute Quantification 5 Experiment Report This section describes how to print experiment report and covers report a template designing and print setting Design experiment Prepare for the reaction Run the exp
27. 37 1 1 d zr 47 9q WOO AH ON LS AH 9 AS Nd9 1 17 0000055078050 saod 0000125078050 0000125078050 0000165078050 A8 0000125078050 NVI cms 401044 492244 119 dv O4d4 SO 87 iagram D iring LineGene9600 LLINd 478944 f NI3QONV 9 1NO9A GEF LJ3HA v er WOO E Er cer LER WOO AZL dvr Oodd vf OlLdO 0000212078090 Ag WOOD 8 2 114 ON 9 2 11 ZLNOON WOOD Zf LINd AZL 2 2 dreds ir WOOD 8 1 114 WOO LNISGONV 011 ON 9 Lf LINd 431 NO LLNOON S Lf LNd 2031 NO 011 LJd3HA 1047 NO WOOD Lf LINd AZL L LP LINd 081 031 2031 11 031 2031 11 1031 11 031 SQ31 0008112078050 Ag vldod SINd 0005115078059 401944 NISY ASIA 0000 2E078050 01994 AND 8 1 NOM Lf an oir SOA 9n Linvs vie elf AND lt 9 12 na N3dO ANMLOH 9 V amp fOLOG I 9142 SIF aan S ver 2 4 22 na dOLOGI 97190
28. AH_8 rer AH 47 9 27 AH_S vzr bzr AH vZf IN3S AIT daLSEW 9 5 02 AGEN 6 0 AH AH 931540 9 91 S 8Lf 81 8Lf AH 20 807 daLSLN 9 9Lf AS NdD S 9Lf 9Lf AH 9 E rir ON AH cz AH 1 21 110 NO 6 9 ON 2 97 L9f YALVSH AH ON 2 AH 491944 NJS Ser 496044 119 MOd 6r 496044 119 MOd 8f GEZr 9 va Scar tier pst 2 Lzr Za 61 2 6 LO E 037 AH ELLE ld 01 AH 1 00 AH 2 2 AH 1 20 492044 rer val zr 631 ter zn 4 S r 47044 SO ndO vf AH t f AH E L f AH MOd 982d 9 t Lf GAH Lf d vir zr vr SE aai zr 047 3 vir d31 0
29. ARTING SOFTWARE INTERFACE de 6 CHAPTER 4 ABSOLUTE QUANTIFICATION co 8 DESIGN EXPERIMEN T 555650 088524 dci c os ne 8 Create New Absolute Quantitative Experiment 9 22 0 111 er 9 L3 Sample Infottinatton Seine ue 2 10 14 Reaction Plate Sen de 11 1 2 ling ee nee ee 13 2 PREPARE FOR REACTION il ico 15 D RUN TE EXPERIMEN Tune ld 16 east erlebe 17 325 Start ad 17 e ia WO cs 18 A ee ehe 19 Fe EXPERIMENTANATLYSIS 15 5 65 iio 21 MAA 22 42 Adjust Re ANAL 15525256 21 EXPERIMENT 29 521 Report Template DESIRE laci n 30 SAPNE 30 LineGene9600 Contents 3 9 Report PTN INE a 31 Oi ATA ea 32 Gell Export Aa M eta 33 6 2 Experiment 9 22 Rah 33 6 3 Export Experiment 1 ile An At 33 CHAPTERS USAGE a 34 de 34 2 TARGET LIBRAR an o a M 34 CUSTOMIZED DYES KP EUM
30. C HU O SE O C DU aYa C a ala a a ala NA AQ 6 a Cy a t AQ IZ O CAM C O CAM 7 C Jw wu C 3 a a aa a Mn AU AU O C IZ AU C O w AU C C Mn a a aarala aYaYala Araia 1 D Tis O C w C w v w v gt OO Ou C C O V V T NO a ala NM a a a Ma a a ANA AQ a MOK a MQ CA VA v v v C v C v CA WV IZ C w FB 02 5 Baseline Measurement B Fluorescence Calibration D Best Gain Measurement G Best Gain Dyes Measured M Crosstalk Measurement R Crosstalk Dyes Measured T Please input password ZELLEN 41 LineGene9600 Absolute Quantification 5 Gain Setup Channel Gain Best Gain un A bremA 5 5 5 42 LineGene9600 Absolute Quantification E C n gt E t AIO Block Scan Method Line Scan Setup MIB MID MH whole Block Scan 1 Dye Mame clear Customized Dyes Prop Std Unk Meg Pos Bk Group No Concentration Groups Unit a aida a aya C v N
31. IVD medical equipment Fluorescent Quantitative Detection system LineGene 9600 Series User s Manual Im Hi Attention Users are recommended to carefully read the contents of this manual before operating the Bioer Fluorescent Quantitative PCR Detection System e To carefully observe all special Warnings and Cautions outlined in this manual This manual should be maintained properly in good condition for reference Caution Copyright reserved The Bioer Co reserves the right to modify this manual at any time without notice The manual contains copyright protected and patented material Without prior written consent from Hangzhou Bioer Technology Ltd any part of the manual shall not be duplicated reproduced or translated into any other language Thank you for your purchase of this product Before initial use of this instrument please carefully read this manual File No BYQ60840000000ESM File Version January August AugustMarch May 20120 Version 1 4860 LineGene9600 Important Notes Important Notes 1 Usual practice Announce Very important information is contained in any item and should be carefully read Failure to operate instrument according to the instruction would result in damage or abnormal function of instrument Warning The warning message requires extremely careful operation of a certain step Failure to observe the instruction would result in serious per
32. J LA 1n nm m LA LA NS am M VA v gt na WNhala Ria La 4 X w LA LA a a LA LA 3 ON gt LA gt LA M LA hd LJ KJ ala alfa LA A LA A lt Cancel C 43 m Group 1 1 Group 2 2 Group 3 3 Group 4 4 SYBR S Cy3 C TAMRAT NED N ROX R TexRed T cys LCRed L FTA F9 Cr c an nn nn Mana an LA Vu LA w VA ALO ah Ria Mathad LA MJ 2 LA WA Y 14 w M D Mate a man vin LAE LA LA LA Vul AA LA M na Ok na man A DANO A LA V w A A d _ Nhala Bla n NO A V V ww gt gt LA LA LA LA LA m aida ala nn 7 A alallaYa LA AS w LA Line Scan Setup Hs Cancel C whole Block Scan LineGene9600 Absolute Quantification U a a v I 7 E IJI V O gt 3 me Files of type Baseline Para Files Cancel S Look in e Er Chinese baseline600003 b96 2 baseline b96 ES a cal 4
33. al holes of the module cares shall be take to prevent cleaning agent from dropping into the well 3 The surface of this instrument shall not be cleaned with corrosive cleaning agent 4 order to avoid to scratch or damage optics in the wells sharp and hard things are prevented to reach to wells of block Diary R 81 LineGene9600 Maintenance 2 FirmWare Choose Port COM The power switch at rear of instrument is set ON but the instrument makes no response The module temperature runs normal but the fluorescence detection makes no scanning Display of system parameters menu requires input of Password The run switch at front of instrument is not pressed which shall be set On during running otherwise the inside switching power will have no voltage output The fixing clamp is not removed or is not inserted and tightened to rear UNLOCKER well The system parameters are for instrument manufacturer s inside calibration and require special accession password 82 For working of instrument its run switch shall be pressed and the green loop lamp shall light on to indicate energizing of the control system Remove fixing clamp and tighten to UNLOCKER well For transport the instrument shall normally shutdown and fixing clamp shall be removed and then screwed into side well The function is unnecessary for the user and for calibration it shall contact m
34. anufacturer s service personnel LineGene9600 After turning on power switch the power lamp fails to light on At detecting sample position the step motor fails to work and the communication fails After detecting sample position the actual temperature displays 0 C or 100 C Module temperature raising lo wering rate obviously decreases or temperature control is incorrect The run switch is not pressed Maintenance This switch is used for temporary turning on off output power and is equivalent to standby function For prolonged shutdown the rear power switch shall be set Off Power 15 not switched on Burnt fuse Damaged switch other Poor contact or damage of interface wire The power switch is not turned on or is turned on only after Check and switch on power Replace fuse 250V 8A 5 20 Replace switch Contact the supplier or manufacturer Check connect or replace interface wire Turn on power switch and restart program program running The step motor or the drive is damaged The fixing clamp is not fully inserted The module temperature sensor is damaged lt accompanies panel red lamp alarm and software prompt and instrument automatically stops running The power switch is turned on only after program running The program is searching communication port and during this period data would be not sent Contact the s
35. asured Options for System Alarm and Tips 0 Load Customized Parameters P Save Customized Parameters U Copy Baseline Parameters to Install Directory L Copy Crosstalk Parameters to Install Directory S Options for System Alarm and Tips O Copy Best Gain Parameters to Install Directory B Copy Fluorescence Calibration Parameters to Install Directory C Load Customized Parameters P Save Customized Parameters U File Info N Insert Cal 0 Copy Baseline Parameters to Install Directory L Crosstalk Parameters to Install Directory S Export Raw Data Q Copy Best Gain Parameters to Install Directory B Export Cal Para P Copy Fluorescence Calibration Parameters to Install Directory C A Firmware Upgrade F Firmware UpgradefF Diary R Diary R j System Parameters pull down menu 53 LineGene9600 Absolute Quantification 3 1 Preparation for reagent sample e Preparation for reagent LineGene 9600 series fluorescent quantitative PCR detection system adopts 0 2ml centrifugal test tube to place reagent sample and 10 50 is suggested for sample optimal reaction system e Optic lucid tube on bottom such as normative single valve calandria full apron plate and so on are allowed in strument e Centrifugal operation Before placing into the instrument test tube with reagent sampl
36. ation Folder Look in LineGene 9600 v Folder name Program FilesibioeriLineGene 96001 InstallShield s LineGene 9600 InstallShield Wizard Custom Setup Select the program Features you want installed Click on an icon in the list below to change how a Feature is installed inssene 9620 Feature Description E3 LineGene 9640 E y LineGene 9660 E y LineGene 9680 This feature requires OKB on your hard drive InstallShield LineGene LineGene LineGene 9620 9640 9680 oo LineGene9600 Preparations Selection oftware installation depends on detection channe ofn mer Alhen Inn 1 ja CY n rine f in Inn in ar LA LA J 5 waw waw LA LA J LA J LA LA LA I LA Ta Alalare AI QUAM A in Inn a YLT Ga Ta I uL LA I LA v gt v LA ww gt v te Stet HaAmsnusah e telsshor stemtelschs seaee Cation tA le B modification oaramete are n olde 7 1 a Ta 6 4 Ej a MAYA f_ 1 a A 1 4 arava a NT 1 LA LA LA v LA y y LA LA I LA 7 LA ila NN a Cel ANION AF Y 1 Ta Ta NN Aira NA LA AS LA LA SIS LA LA LA LA LA LA LA LA AS Caution2 Afte Ta IND
37. by a Clicking File New on the menu bar b Clicking New on the toolbar 15 LineGene9600 Absolute Quantification File Instrument Tools Report Data Summary Help New m Save Y Export New Experiment 1 2 Detector Setting e Click Setup Detector Input experiment properties Input the experiment name user name and comment at the experiment properties column netu 20111117 Experiment remark Comment EM CS Detector Setting Set up the Detector Assay Dye and Color x If necessary the user can also a Add detector b Add assay c Delete detector d Delete assay Add the detector in the Detector Library click Add Detector Form Library the Detector Library window will pop select the Detector on the window to be added X The user can also conduct Add Modify and Delete operations in the item library 16 LineGene9600 Absolute Quantification Detector Library Detector Target3 Target4 f Set up the detector set up the assay set up the dye name and set up the color Detectors Detector Assay _ Dye 22 Color Target __ Set up reference dye Reference Dye VIC 1 3 Sample Information Setting Click Setup Sample e sample information a ltemized addition input ID in Sample ID gt press Enter add one piece of sample information b Batch addition click Batch Add the Batch Add wind
38. ck Data Summary Export to Database the save file dialog box will pop save the exported database file 6 2 Experiment Filing e Click Data Summary gt Archived Experiment Diretory gt the Experimental archive storage directory window will pop up set up the storage path of file Experimental archive storage directory Experizents in the following archive directory D LineGene9600 Change e Experiment filing Click Data Summary Archived Experiment export the filed experiment file X The suffix of filed experiment file is 6 3 Export Experiment Data to EXCEL Click Data Summary Export Experiment to EXCEL set up Well Horizontal set up Well Vertically gt the exported experiment data will generate EXCEL file 71 LineGene9600 Tool Usage Chapter 5 Tool Usage 1 Gain Setting Gain Setting tools are used to set up gain modes Click Tools Gain Setting the following window will pop up Gain setting can be set up as reference gain custom gain and auto gain In the mode of Custom Gain the user can modify the gain value Gain Setting 2 Target Library Target Library tools are used to set up the inspection libraries of absolute quantitative Click Tools Target Library open the following window The user can a Add Detector b Modify Detector c Delete Detector Detector Target3 Target4 3 Customized Dyes Customized Dyes tools are used
39. ck corresponding hole site curve X The default is all hole sites are selected b Zoom In Zoom Out and Reset the reaction plate c Check well table information d Check results summary Plate Setup Well Table e Setup assay a Set up Assay b Set up Threshold c Set up automatic baseline When the threshold value is not automatic the user cannot set up the automatic baseline Target RETE 2o LE 0 5 61 LineGene9600 Absolute Quantification 4 1 2 Check Standard Curve e Click Analysis Standard Curve Amplification m Plot 2 Standard Curve Melting Curve e Check standard curve a Set up assay Concentration Assay Targetl FAM Intercept 46 510 3 24 Error 0 009 Correlation 0_ 998 E ficiency 103 54 e Check the reaction plate a Select relation plate hole site and check corresponding hole site curve X The default is all hole sites are selected b Zoom In Zoom Out and Reset the reaction plate c Check well table information d Check results summary 62 LineGene9600 Absolute Quantification Plate Setup Well Table 4 1 3 Check Melting Curve e Click Analysis Melting Curve Melting Curve e Check the melting curve a Check the fluorescence curve b Check the derivative curve c Set up color 63 LineGene9600 Absolute Quantification Caer o o v o 3 60 62 64 66 68 7
40. ction of 96 well Fluorescent Quantitative Detection System 1 Applications 96 well fluorescent quantitative PCR detection system adopts amplification of fluorescent real time detection mode analysis PCR module and is suitable for polymerase chain reaction fluorescent quantitative detection in research fields of human genome engineering forensic cancer tissue population biology paleontology zoology and botany and in clinical diagnosis of virus cancer and genie diseases PCR detection system belongs to IVD medical equipment which is to use polymerase chain reaction means to quantitative analysis of different genes in clinic lab 2 Features Novel and human orientated running and operation interface make operation much smooth he adopted fluorescent real time detection mode realizes simultaneous amplification and detection in a same tube without need of post treatment Advanced thermoelectric refrigeration technology ensures fast and steady heating and refrigerating of ultra fast heat cycles system Multi point temperature control ensures much even temperature of 96 sample holes e t can control the temperature gradient of 4 areas through setting e Stable and correct 1 36 C gradient function makes PCR condition optimal he constant temperature function of SOAK realizes low temperature storage of PCR reagent e t uses maintenance free LED excitation light source of long service life he advanced optic fiber transmi
41. e shall be subjected to centrifugal operation to ensure that the reagent is at test tube bottom and the inside is free of bubble 54 LineGene9600 Absolute Quantification e insert test tube If the sample quantity is less than well quantity of module the sample test tubes shall be evenly distributed in module well as far as possible so as to make the hot lid steadily compress on the top of test tube during running The load of module shall be even so as to ensure even and consistent temperature change of each test tube The sample is at reaction The sample test tube requires test tube bottom sufficient speed At centrifugal operation it requires sufficient centrifugal time Correct position of sample in test tube 3 2 Start Run e Click Start Run The default running interface is florescence curve Run Status e Operating confirmation a Modify hotlid temperature and liquid quantity b Gain parameter setting c Target florescence value setting x If necessary the user can modify above parameters directly in this window FOR Confirm 2220 Warning Without the baseline parameters the fluorescence data will not be calibrated Reference gain of Dye does not exist has been replaced with the default gain 105 Liquid Quantity 40 Gain Auto Gain Used Gain F1 7 Use Reference Gain e After it starts running the user can a Skip
42. e click short cut icon on desktop p LE p LineGene 4 a 0 LineGene LineGene LineGene 3620 9640 9660 3 Starting software interface 10 LineGene9600 Start 11 LineGene9600 Start Power Options Properties Power Options Properties f Power Schemes Advanced Hibemate UPS Power Schemes Advanced Hibernate UPS Select the power scheme with the most appropriate settings for 4 j When pour computer hibernates it stores whatever it has in this computer Note that changing the settings below will modify memory on your hard disk and then shuts down When your the selected scheme computer comes out of hibernation it returns to its previous state Power schemes n Hibernate Home Office Desk v 4 Enable hibernation Settings for Home Office Desk power scheme Disk space for hibernation Free disk space 13 285 MB Disk space required to hibernate 760 MB Turn off monitor After 1 min off hard disks Never System standby Never Run the program and it will display the system window 12 LineGene9600 Start LineGene9600 20111209 New save e Export New Experiment FA Open Recently Used Templates Categorized Templates The system window consists of the menu bar the toolbar and the main form 13 Lin
43. eGene9600 Absolute Quantification Chapter 4 Absolute Quantification 1 Design Experiment his section describes how to design absolute quantitative Start experiment and covers new absolute quantitative experiment inspection item setting sample information setting reaction plate setting and program setting Design experiment Prepare for the reaction Run the experiment Experiment analysis Experiment report Data export LineGene9600 Absolute Quantification he methods to build a new absolute auan ation detection proaram file mair nelude the Afte Absolute Quar ation button of Startina so Are interface refer to Fic e Untitled fgdpcr IM File F Sample Data S System Parameters P Analysis Options amp Data Capture Curves C Help H 7 Sample Data Run Programs Quantitative Analysis Melting Curve Analysis Data Query EdHotlid C Liquid Quant ul Sample Mode Rate 2nd Temp Step Size Step Delay Grad Temp SN Programs Notes gt Cantina End gt lt p 2 Note that all channels are allowed with LineGene 9680 800001 I j He1 1 Create New Absolute Quantitative Experiment Click build New Experiment on Home interface and create absolute quantitative experiment window X Absolute quantitative experiment can be also created
44. ent immediately after unpacking when instrument is sent to repair department e For the damage of instrument occurring during transporting to the repair department due to improper package or incorrect place of fixing pin Hangzhou Bioer Technology Co Ltd shall bear no liability LineGene9600 Contents Contents CHAPTER 1 GENERAL DESCRIPTION ee cee eatin ea 1 APPLICA TIONS D c 1 PR 118 22250150 81105041 uM I dM MI ni iu 1 3 JINOTBS TO MODEL AND DESCRIP TION os id 2 PERFORMANCE PARAMETER SA 2 5 GENERAL DESCRIPTION OF FUNCTIONS OF THE NECESSARY 3 CHAPTER 2 PREPARATIONS ien keds eme tado 4 1 TRANSPORT AND STORAGE CONDITION OF eene seen enun 4 2 INORMAL WORKING CONDITION 2 ie 4 3 PREPARATIONS BEFORE START OF 2 enhn nennen 4 SER Mova LOr Di Sn 4 3 2 Connection of power wire and communication Wire 5 4 SYSTEM INSTALLATION AND UNLOADING ie ee 5 ES ODE denti terio 5 ASI ra 5 CHAPTERS START eun duet iniu nt 6 l CHECKS BEFORE START Na 6 PAP Uu p ende 6 Js ST
45. eriment Experiment analysis Experiment report Data export End 67 LineGene9600 Absolute Quantification 5 1 Report Template Designing Click Report Report Template Editor the Report Designer window will pop up The report consists of controls and the user can add modify and delete controls Available controls include Static Text Dynamic Text Line Static Image Aamplification Curve and Quantification Aanalysis Result Report Designer D XLineGene960 XScientificXconfigXreportXAbsoluteXdefault rpt TER New Open Save Preview view Delete Selected Controls E Common Controls gt Static Text Hospita 1 Dynamic Text Static Image 5 Report Line Amplification Curve Name Name Sex Sex Age Age Hospital No HospitalNo Quantification Analysis Result Known Controls amp Static Text Controls E Dynamic Text Controls Test Item Test Result Reference Conclusion Amplification Curve E Appearance Alignment MiddleRight BackColor White Solid 1 False False False Color Black Font Tahoma 8 25pt M r 5 PM DesignVisible True Layout Submitting Date Report Date ReportDate Location Padding Size Text text of the element 5 2 Print Setting Click Report Print Template Setting gt the Print Template Setting window will pop up The user can set up the hospital name re
46. ex Sex Case No CaseNo Outpatient No OutpatientNo Bed No BedNo Hospital No HospitalNo Nationality Nationality Sampling Time SamplingTime Doctor Doctor Dept Department Submitting Date SubmittingTime E 6 Sample Column Library Sample Column Library tools are used in the experiment design phase The user can selection the definition of contents in the drop down box when setting up sample information Click Tools Sample Column Library the following window will pop up The user can a Add columns b Delete columns c Edit the columns content 74 LineGene9600 Tool Usage Sample Column Library Auto Complete Columns Management Auto Complete Columns Current Values e China Doctor Doctor Dept Department Tester Tester Notes Notes Delete Value per Line ED e 7 Instrument Calibration Parameters Instrument Calibration Parameters tools are used to calibrate instrument parameters Click Tools gt Instrument Calibration Parameters the following window will pop up HR Instrument Calibration Parameters Select Instrument 600254 mm Baseline Parameters Measured Reference Gain 5 Proportion Parameters Measured CrossskComecionPa OSFAMHEXROXTAMRA Crosstalk Gain Paramet FLEZFSFAES 8 Measure Crosstalk Calibration Para
47. fixing pin on its side and insert and tighten the lock into unlocker hole at its back otherwise the instrument would be unable to make fluorescence scanning For removal of fixing pin refer to chapter 1 3 1 Removal of fixing pin 1 Checks before start After inserting the power plug and energizing this detection system the followings shall be checked e Check the voltage of power supply for consistency with system required one e Check the power wire plug for correct and reliable insertion to power socket e Check the communication converter for correct insertion into the host and tightening and the lead plug for correct and reliable connection to the computer e Check the update shift switch MODE of communication converter for being at normal state e Check the environmental working environment and the storage condition for meeting requirements 2 Start In order to ensure effective communication connection between the instrument and the computer system the system shall be started in the following sequences 1 step Start computer display and host 2 step Turn on power switch on instrument s rear bottom board 39 step Press Run Switch on front cover to set the system at running allowable state 4 step After entering operation system start LineGene 9600 fluorescent quantitative detection system For starting software click LineGene96200 LineGene9640 LineGene9660 LineGene9680 from Start program menu or doubl
48. g of software and preparations before start of LineGene 9600 series fluorescent quantitative detection system 1 Transport and storage condition of instrument Environmental temperature 20 C 55 C Relative Humidity lt 80 2 2 Normal working condition Environmental temperature 10 30 Environmental RH lt 70 Power supply 100 240V 50 60 2 600W Caution Before use the instrument please make sure the working condition meets the above requirements The power socket shall be 3 holesocket and reliably earthed 3 Preparations before start of instrument 3 1 Removal of fixing pin In order to prevent the inner moving parts from shift and collision during transport before leaving the factory they are fixed with fixing pin After the instrument is placed on the desired location the fixing pin shall be taken out through counterclockwise rotation inserted to unlocker hole at its back and fully tightened to release protection of the inside protective switch Only after correct installation of the fixing pin may the instrument be energized otherwise the instruments temperature control system is able to run but X Y axis scanning system doesn t run Fixing pin Can be removed through rotation Please keep the fixing pin tightened Before moving instrument turn the power pending 4 LineGene9600 Preparations completion of self test action then shut down instrument normally and take out the fi
49. g running of program the PCR detection system running program shall be terminated the instrument shall be switched off and then re started 58 LineGene9600 Absolute Quantification 4 Experiment Analysis This section describes how to view the experiment analysis result after Start the operating experiment and adjust parameter reanalysis This section covers the analysis of amplification curve and standard curve adjusting parameter reanalysis and parameter importing Designed experiment Prepare for the reaction Run the experiment Experiment analysis Experiment report Data export End 59 LineGene9600 Absolute Quantification 4 1 Check Results 4 1 1 Check the Amplification Plot e Click Analysis Amplification Plot Amplification O Plot Standard Curve Melting Curve e Check the amplification curve a Set up color b Set up plottype c Set up show dye gt When the background color of a dye name is blue it means to be displayed while white indicates not to be displayed Amplification Plot Color Well PlotType Linear Show HEX ROX 5 4500 4400 4000 3600 Fluorescence 3200 2800 2400 2000 1600 1200 800 400 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 Cycle 60 LineGene9600 Absolute Quantification e Check the reaction plate a Select relation plate hole site and che
50. l maintenance personnel Replacement of elements under energizing state shall be prohibited c Use of power supply Before turning on AC power make the power voltage consistent with the instrument required one 100 240V 50 60Hz and make the rated load power socket not below instrument s maximum load 600W d Power wire LineGene9600 Important Notes Generally this instrument uses accompanied power wire The damaged power wire shall be replaced by one of the same type and same specification It can t be repaired The power wire shall neither be compressed nor be laid in gathered personnel location e Insertion and withdrawal of power wire At insertion and withdrawal of power wire the operation portion of the plug shall be correctly held with hand At insertion the plug shall be completely and tightly inserted into the socket and at withdrawal heavily pulling the wire shall not be allowed f Placement of instrument This instrument should not be laid in the place where it is different to cut off the power supply This instrument shall be fixed in a low RH and low dust place apart from water source e g water pool and water pipeline and the room shall be well ventilated and free of corrosive gas or and interference of strong magnetic field The instrument shall not be placed in a wet or dusty location and its working table shall be level and steady The openings on this instrument are for ventilation circulation and in order to av
51. meters Measure Crosstalk Calibration Parameters tools are used to measure crosstalk correction parameters Click Tools gt Measure Crosstalk Calibration Parameters the following window will pop up The user can add and modify the channels to be tested and dyes according to his needs 5 LineGene9600 Tool Usage upload corresponding reaction plates and operate the experiment when the experiment is over the system will automatically save the crosstalk correction parameters Experiment Type Absolute Experiment Name Crosstalk Parameter Measurement Experiment Properties 2222277 Crosstalk Parameter Measurement Comment 9 Crosstalk Gain Parameter Measurement Crosstalk Gain Parameter Measurement tools are used to measure crosstalk gain parameters Click Tools gt Measure Crosstalk Gain Parameters following window will pop up The user can add and modify the channels to be tested and dyes according to his needs upload corresponding reaction plates and operate the experiment when the experiment is over the system will automatically save the crosstalk gain parameters 76 LineGene9600 Tool Usage Experiment Type Absolute Experiment Name Crosstalk Gain Measurement Experiment Properties 10 System Maintenance System Maintenance tools are used for system maintenance Click Tool Menu System Maintenance the Password Input box will up input the correct passwords cond
52. n 5 attribute exos areAn e same chanmaeL anea heyre dnmesoessan e re select JOE HEX TaxBad LCRed 4 mu mm mm m mim un rv 548144 ier Jus age Lone A a a an Lia a ala a LA w A LA ues 1 LA P hd LA A NA LA i LA LA a a an n M aire v w LA w VA LA Y Y 19 vA w LA LA LA LA A A LA VA 5 J OO AOS SOAP DOCS CH OG LO HG 2568526 1 5 2c 2138 37 LineGene9600 Absolute Quantification i x y Esus ggg TE al Green 128 Define Custom Colors gt gt 5 olid Lum Blue fdo Custom Colors 38 LineGene9600 Absolute Quantification 5 39 7 C L2 v v I a Ar a JU To v 7 OO Na OUT SIT ATOUT VAS ala v JU anta tu LineGene9600 Absolute Quantification a a f E 116341 dh
53. oid over head of the instrument body they shall not be blocked or covered When a single set or several sets of instrument are used the interval between its surrounding ventilation opening and its nearest object shall not be below 30cm or 50cm Excessive environmental temperature would impair the test performance or result in fault of instrument This instrument shall not be used in location subjected to direct sunshine or strong radiation of light source otherwise its fluorescence detection would be impaired The instrument shall be kept away from hot gas strove and all other heat source At shutdown the power shall be cut off If the instrument is to be set aside for a long time the power shall be cut off the power plug withdrawn and the instrument covered with soft cloth or plastic film to avoid entrance of dust and foreign matters g Notes during operation During test cares shall be taken to prevent liquid from dropping onto the instrument The castoff used in test such as consumables reagent and so on should be treated as require and should not be thrown away or poured During test if there is hazardous substances user must be trained before using Hazardous substances which has been used should be coped with and saved according to derection for use User who operates the instrument must be trained and has relevant quantification Caution In any of the following cases immediately cut off the power supply withdraw the
54. ow will pop up 17 LineGene9600 Absolute Quantification Batch Add Start Sazple Id e Delete sample information a ltemized deletion select one sample click Delete delete the selected sample information b Delete all click Clear All gt delete all sample information e Import Export sample information a Click Import Sample Info the File Import window will pop up gt import sample information file in CSV format b Click Export Sample Info the Save As window will pop up the sample information will be exported in CSV file format Sample ID E Batch Add Delete Clear 11 Import Samples Info Export Samples Info e Setup sample information Sample Id Color Sample Name Name Sex Age Case Outpatient No Bed No Hospital Nationality Sampling Time Doctor Dept Submitting Dat al Samplel Hu M 25 C1 01 Bl Hi China 2011 12 14 Doctorl Dept 1 2011 12 14 a2 gt Sample2 Wang 30 China 2011 12 14 Doctor2 Dept 2 2011 12 14 Sample4 China 2011 12 14 Doctor4 Dept 4 2011 12 14 a5 Samples Zhao 45 C5 05 B5 H5 China 2011 12 14 Doctor5 Dept 5 2011 12 14 Reaction Plate Setting e Click Setup Plate Setup Detector e Setup the inspection items of reaction plate __ a Select reaction plate hole site click Reaction Plate Hole Site Apart from reaction plate hole site the user can also right click the reaction plate hole site to Co Paste and Add New Detec
55. p lights yellow which denotes that the module is not fully pushed in and the hook is not fastened e Error The indication lamp lights red which denotes that the instrument is in troubles Caution For prolonged shutdown after end of program running set the power switch at instrument s rear bottom board at OFF and cut off power supply After turning on power the hot lid and module will run as per default state The front cover of instrument is fixed with a self locking key to control energizing of its inside control system e Run Switch running standby switch e After pressing this key the green loop lamp lights on instrument inside system is energized and the user is allowed to run the program e After pressing this key again the key will spring out the green loop lamp lights off instrument inside system is de energized and the system is under standby Caution The run switch is for user s easy operation and is merely used for temporary or 57 LineGene9600 Absolute Quantification short time closing control system When the system is under standby state the instrument inside AC circuit is live 3 4 1 Prompts likely occurring during running Caution Hot lid temperature sensor alarm prompt Sink temperature sensor alarm prompt Environmental temperature sensor alarm prompt Module temperature sensor alarm prompt Module sensor short circuit or short circuit alarm prompt In case of temperature alarm display durin
56. port name reference value tester checker amplification plot setup default report template and paper size Print Template Settings Absolute Template Se Hospital Report Reference Tester Checker Amplification Plot Setup Legend 9 Color LineStyle Frint Setup Default Report Tezplate default same Paper Size Ai Printer Use Default Printer O Use Custom Printer 68 LineGene9600 Absolute Quantification 5 3 Report Printing e Click Report Report Print o Report Print e Report print setting a Set up report template b Print setting please refer to Section 5 2 c Select print items d Print preview e Print the report Report Template M Print Preview Print One PerReport Print Sample Id Sample Name Test Item Name Sex Age Case No Outpatient No Bed No Hospital No Nationality Sampling Time Diagnosis Notes Mi Assay Item SYBR Detect Concentration 7 82e 06 Conclusion Positive 69 LineGene9600 Absolute Quantification 6 Data Export This section describes how to export data and covers exporting to the Start database experiment filing and exporting the experiment data to EXCEL Design experiment Prepare for the reaction Run the experiment Experiment analvsis Experiment report Data export End 70 LineGene9600 Absolute Quantification 6 1 Export to Database Cli
57. rary opening amplification instrument casing and inspection shall not be allowed In case of above troubles requiring opening amplification instrument casing for inspection please timely contact the supplier or manufacturer 85 Wiring Diagram LineGene9600 Appendix LineGene 9600 series wiring AH 2 pee LA NO W 99d 8 Od NO ON 9 NO S ENO LOS ENO NO S S LENS 0L ZNO 224 6 ZNO S WAL 89 9 ZNO S ZNO LNO S WN ZNO LNO 5 L ZNO LNO 994 OLLNO Dans 6149 S 8 1N9 Wal ZENO 49 9 LNO SLND V LNO N LNO S _c NO W b LNO gt AH AH 4009 AVGOd gt LMS 009 2109 08 076 001 1 4 44104 35 25 0 9 VEAOSTIV 15 4 JSN us SO wWWOZG VEAOSTIV 13SN3 Inlet with fuse 86 iagram D iring LineGene9600 WIL_ZL NO LL ZNO LHJOQOV ddOGOV 6 59 8 2 0 89 9 ZNO S ZNO t ZNO ZNO 614 2 S1d L ZNO 00477 410 5 410 5 410 2 5 440 1 5 4045 HalvaH 8 1 A17 HalvaH 94r 831V3H 6 0001517078059 84594
58. sonal injury 2 Safety During operation maintenance and repair of this instrument the following basic safety notes shall be observed In case of failure to follow these measures or the warnings or notes indicated herein the basic protection provided by the instrument its safety criteria of design and manufacture and its predicted use range would be impaired Hangzhou Bioer Technology Co Ltd shall be irresponsible for the consequence resulted from user s failure to observe the following requirements Note The instrument complying with the Standard GB4793 1 IEC61010 1 is a general instrument of class the protection degree is IP20 It is intended for indoor use Note The instrument complying with the Standard 0648 61010 2 101 is used for IVD medical equipment a Instrument earth In order to avoid electrical shock accident the input power wire of instrument shall be reliably earthed This instrument uses 10A 3 core earth plug and this plug is provided with a third earth leg is merely used for earth type power socket and is a safety unit If the plug is impossible to insert into the socket the socket shall be correctly fixed by electrician otherwise the earth plug would lose safe protection function b Keeping apart from the live circuit The operator shall not arbitrarily remove instrument protector replace elements or make inside adjustment and the task if necessary shall be made by qualified professiona
59. ssion technology makes photo electric detection system much sensitive and reliable Precise optical path system and ultra sensitive PMT system render best correctness and sensitivity of fluorescent detection e t can make real time dynamic monitor of the entire PCR amplification process e t has high linear range up to 10 orders of start DNA copies without gradient dilution e PCR reaction tube is unnecessary to open which protects products during and after PCR from contamination and ensures correct results he multi color fluorescence monitor may acquire much information from a single reaction e The hot lid technology realizes oil free operation of PCR Automatic hot lid technology needs no manual opening closing and ensures constant pressure of hot lid used in different high test tube e t has full Chinese interface and the functions of flexible setting of program and overall analysis and report and can store all parameters e t can print out several or a single sample report 1 LineGene9600 General Description Automatic correct and timely remote networked services provide 96 well quantitative detection system the latest technical support e The advanced module bottom fluorescent detection technology is protected by invention patent 3 Notes to model and description Model 96 Design series number Specification code 96 denoting maximum sample quantity 96 Abbreviation for Fluorescent quantitati
60. tion Double click LineGene9600 installation LineGene9600DiagnosisSetup exe display the installation interface select installation language set up installation path install Operating system e Double click the LineGene9600 shortcut on the desktop e Click the start menu Program LineGene9600 4 2 System Unloading Control Panel Add Delete Program LineGene9600 Unload LineGene9600 Preparations Choose Setup Language Select the language for this installation from the choices below Engish United States Welcome to the InstallShield Wizard for LineGene 9600 The InstallShield R Wizard will install LineGene 9600 on your computer To continue click Next WARNING This program is protected copyright law and international treaties 5 LineGene9600 Preparations fis LineGene 9600 InstallShield Wizard Customer Information Please enter your information User Name Organization Serial Number Install this app 9 Anyone who uses this computer all users O Only For me 13 1 9600 InstallShield Wizard Destination Folder Click Next to install to this folder or click Change to install to different folder Install LineGene 9600 to D Program Files bioer LineGene 96001 LineGene9600 Preparations s LineGene 9600 InstallShield Wizard Change Current Destination Folder Browse to the destin
61. to set up existing dyes and newly added dyes Click Tools Customize Dyes open the following window The user can a Create dye b Modify dye name and channel c Delete dye 72 LineGene9600 Tool Usage d Move dye upward e Move dye downward x After adding new dyes or modifying dyes the user should conduct crosstalk parameter measurement Custonized Dyes 3 FAM Channel 1 470nz 525nm mn 1 Bin e Dye after you add or modify parameters for crosstalk measurement E 4 Customize Columns Click Tools Customize Columns the following window will pop up The user can a Add columns b Delete columns Modify column name Customize Coluans MCE Delete 73 LineGene9600 Tool Usage 5 Column Selection Select Columns tools are used to add the new columns in above section into current existing columns or remove existing columns in current column Click Tools Select Columns the following window will pop up Current existing column items include sample absolute report absolute report setting query and query condition b Double click column can add or remove a column c Column with indicates it cannot be removed Select Columns 3 Unselected Columns Current Columns Item i ItemOO Query Condition Absolute Report Setting Sazple Absolute Report Query Sample Id Sampleld Color Color 3 Sample SampleName Name Name S
62. tor Adding new detector will open the Edit Detector Library window 18 LineGene9600 Absolute Quantification Edit Detector Library Detector Name Target b Select Assay item and modify the property concentration and concentration unit Concentration Concentration unit Unknown Standard c Select a sample and the list TUM will change d Zoom In Zoom Out and reset the reaction plate e Sample Auto Arrange f Check Well Table Detectors Plate Setup Well Table Zoom In Zoom Out Reset Sample Auto rrange B Target 1 FAM G m M 2 3 4 5 6 7 8 9 Concentration Unit v 4 Samples Show Columns y E ID y Name CC CNN E pm e m e _ m 101 19 LineGene9600 Absolute Quantification Flate Setup Well Table Well Assay Item Property Std Con Sample Name T tt 1 5 Program Setting e Click Setup Program e Run Programs Setup a Create new stage the user can create new Hold Stage Cycling Stage or Melting Stage The user can also click Add Stage directly and the default will be creating new Cyclin Stage b Create new step the user can create new step Before or After the currently selected ste The user can also click Add Step and the default will be adding new Step at the end of currently selected stage or after currently selected step C Delete the user can delete c
63. uct the following setting 11 a Y axis commissioning Password b X axis origin calibration Flease input password Machine serial number setting aca OK Cancel d Photomultiplier setting e Runtime zero clearing f Background measurement g Reference gain measurement h Fluorescence incremental calibration Firmware Upgrading Firmware Upgrading tools are used to upgrade the firmware The instrument by connection of RS232 communication wire with computer realizes its software update as follows Set its rear MODE update switch of communication box on the back of the instrument to right side Update turn on power supply and connect serial port line The instrument is in update status In the panel indicator light and trouble light are lighted in the same time which is normal Click Tool Menu gt Firmware Upgrading gt following window will pop up The user can 17 LineGene9600 Tool Usage a Select serial ports b Select the BIN file to be upgraded c Upgrade Firmware Upgrade Choose Port 1 Upgrade File E PCR bin 12 Convert Old File into New File Convert Old File into New File tools are used to convert old files with the suffix of fqj or fqs into new files with the suffix of fqd Click Tool Menu Convert Old File into New File the following window will pop up The user can a Add files to be upgraded b Delete selected files c Select the output directory of new
64. upplier or manufacturer Tighten it to UNLOCKER well and re turn on power after shutdown Contact the supplier or manufacturer Turn on power switch and restart program If the trouble still exists after researching contact the supplier or manufacturer The ventilation opening is Clear the stem of the blocked ventilation opening Loose connection wire The refrigerating sheet is damaged Fan is damaged or fails to run The temperature sensor is damaged 83 Contact the supplier or manufacturer LineGene9600 Maintenance Inside of instrument is damaged Contact the supplier or The refrigerating sheet is manufacturer damaged Waiti il hot li The module fails to u temperature comes to target heat and refrigerate Au 9 value During hot lid heating up When stopping running module temperature holds down 30 C automatically The running program is After virus killing re install Abnormal infected by virus application software temperature or Computer configuration fails to meet requirements or the Configurating as per require setup of communication port is ments unreasonable fluorescence curve straight line or lose of partial data The module is not fully pushed In case of failure after Yellow lamp on panel NM in and the optic coupler fails to re pushing in contact the lights on detect the module supplier or manufacturer
65. urrently selected step or stage d Display form click Display With Table gt new window will pop gt the details of current experiment will be displayed in table e Set up the experimental data of the hold stage cycling stage and melting stage melting section f Set up hotlid temperature and liquid quantity 20 LineGene9600 Absolute Quantification 105 MAC CET CN 0 5 Run Programs Setup Add Stage Add Step Delete 777 Cycles 40 m Sampling 21 LineGene9600 Absolute Quantification 2 Prepare for Reaction The user should make full preparations prior to the experiment e Ensure appropriate materials are used Start 1 e Ensure the arrangement of PCR reaction plate is consistent with the setting layout of reaction plate in Section 1 4 Design experiment Prepare for the reaction Run the experiment Experiment analysis Experiment report Data export End 22 LineGene9600 Absolute Quantification dddddddddddddddd default FFFFFFFFFFFFFFFSsssss ala a ala a a a a A VA A v w VA V 7 w w LineGene9600 Absolute Quantification 17 5 17 5 Sa EE atan BL ae D gt X BL IH a
66. ve polymerase chain reaction PCR detection system Description LineGene 96 xx Code of ray filter pack 20 with ray filter of F1 F2 40 with ray filter of F1 F2 F4 60 with ray filter of F1 F2 F4 F5 80 with ray filter of F1 F2 F4 F5 F7 F8 Specification code 96 denoting maximum sample quantity 96 English name of description of Bioer Fluorescent quantitative polymerase chain reaction PCR analysis system series 4 Performance parameters opecification FQD 96A model Sample i 96x0 2ml suitable for single tube 8 row tubes and 96 well full apron plate capacity Detection channel M Light Applicable dye SYBR 5 TAMRA TEXAS R Cycler Red Green Quasar705 Temperature range of block 4 105 C Minimum division 0 1 C working Heating cooling 4 0 C s max LineGene9600 General Description lt 0 1 full range 55 typical value lt 0 1 lt 0 ull ical value lt 0 fluctuation da Temperature lt 0 2 full range 55 C typical value lt 0 1 C Temperature lt 0 4 full range 55 C typical value lt 0 3 uniformity Temperature range of hot lid 30 110 C adjustable default 105 C working Repeatability of fluorescent 5 Intensity detection Running mode Continuous running Operati Ra Windows2000 XP Windows Vista Windows7 system Power supply 100 240V 50 60Hz 600W
67. xing pin insert it into right hole of instrument and tighten it 3 2 Connection of power wire and communication wire Connection of power wire The instrument accompanied power wire should be used At connection the instrument power switch shall be at OFF state and after connection the power wire shall be checked for tight contact with the instrument socket otherwise it shall be replaced Connection of communication wire The accompanied communication wire and communication conversion box shall be used One end of the communication conversion box shall be connected with DB15 communication interface at instrument back and another end connected with computer USB interface or RS232C interface or Bluetooth interface After connection screws shall be surely tightened Caution The accompanied power wire is reliable but after several times of insertion and withdrawal the connection would likely become loose In this case the power wire shall be replaced with the one of same type and same specification The communication conversion box is built with special circuits and shall not be arbitrarily opened without permit 4 System Installation and Unloading efnesessary software 4 1 System Installation System Environment e Operating system Windows XP Windows VISTA Windows7 e Runtime environment Net Framework 4 0 e Other software PDF reader Minimum configuration e Processor Intel Core i3 e Memory 2GB e Hard Disc 10GB System installa
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