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SERVICE MANUAL

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2. n 2 JP2 VALVE13 13 BLAST 1 RAE Pci k D 51 25 BLAST rum 3 gt 2 G4 VALVE14 12 STRT 2 y ES m 24V 3 10 24V G2 24 STIR2 15 VALVE2 4 9 VALVEIS ls as 315 VALVE3 G2 52 8 6 G3 2 16 i VALVE4 64 lt i VALVE16 10 Ed VALVES 4 7 T VALVES 22 17 YZ 8 VALVE6 5 MO 5 9 2 VALVE7 lt S1 RED 10 VALVES o Si BLU eu 9 pineut2 lt lt 51 MS 7 ii ON JUMPER BLOCK 12 20 AUXILLIARY Gi Can 19 cont cnt com CONI VALVES 7 o VALVES amp MOTOR 1 3 VALVEIO inputs lt lt S1 YEL x19 12 VALVETI str VALVES 8 9 VALVEIZ lt neo gro Ome VALVEIS HNV VALVE9 2 M VALVE14 oc 22 t 1 VALVEIS
3. 8 7 6 5 4 3 2 1 XMC 0 2 RS4 U56 ust H2 10 ET n be n P X Pis H3 9 XED e pr NED pea 315 o DE X CW CCW g A 6 7SLED avo 01 AA SLED a X_AWOFF Hs 7 SEED Ha 5 BB Y PLS H6 6 6 n BS2 PLS 6 M 14 Y Cw CCw 7 5 S2 PLS 7 m a BS2_AWOFF ia LED YMC2 7 5 5 Y AWOFF 4 52 AWOFF 8 22 BS3 PLS D2 AA XLED Ye vc 8126 v be 51_ 15 3 S3 PLS 941 MM 853 AWOFF 51 9 n BS1 AWOFF m 2 53 AWOFF 8 LED S1_AWOFF 1 e Xo a 19 p3 AA YLED G2 asv jm 47K 5 74HC540 74HC540 GND GND XSWI0 1 YSWI0 41 u52 RDI xswo wl 2242 1 16 xwi 17 i P 3 E 2 15 7 JXHIUM wo 3 14 DINPUTI E Yswo BA y 5 4 13 n NOUM we y 14 b ne 6 n 5 12 3 ANHUM i 13 7 gt 6 n DINPUT2 n RXD BDINPUTO 12 6 8 7 10 DINPUTS 7 A 4 4 BDINPUTI Dou 5 8 9 DINPUT4 BDINPUT2 2 6 BDINPUTS din 470 BS1_PLS gt a 219 BS1_AWOFF 8 b cas on BS2 PLS 74 540 _ b 52 AWOFF BDINPUT O 3 10
4. 8 7 6 5 4 3 2 1 54 15 5000 7 SDI0 7 u61 L 2 ewe mo 5 Pod 42 501 u62 u63 U64 n 502 507 2 19 5 7 sao 10 11 50 sao 10 11 5 0 n me 503 506 ES Be 91 i 56 SAT 9 240 00 1 sb SAT 9 9 m s 3 89 504 505 Bali 92 sas 5 2 D 1 s SAZ 9 s s 20 505 504 98 5 02 i5 553 92 i 506 503 Df 94 i 5 Cas 03 16 504 SM 5 03 i 554 5 Fa 507 502 7 5 95 a 5 SAS s 04 77 95 5 5 57 AM W 10 Per 501 8 06 96 3 w SA6 5 05 18 506 546 95 506 passar RESET 24 5 8 500 97 Du so SAT 3 A5 06 9 507 5 7 s 246 96 9 sor mo a D8 2s 07 07 TOF 14 5410 BSALE n 9 2 74 SAS 8 Ei rmm ae mis xs 12 SAM zs SATI 21 m usn ms 19 SAIS 74HC573 5412 Pu SA12 ms 5414 V SA13 26 SA13 26 SLAWOFF lt 2 m EA GND 52 3 pumx den 8 ais gt 4 19 SRD SAIS 20 SSLAWOFF BDINPUTO 5 E 18 WR TWR 27 ais 25 BDINPUTI 32 7SPSEN 75RD 22 SPSEN q 22 BDINPUT2 7 54 SALE GE 9E
5. 5 o ud 24 k P4 USB Bus COPAS DN 1 EXPANDER 4 2 433 Powered Powered HEARTBEAT A 7 lt lt RI CGRID AM PEI0 7 74LCX16244 7 yy 62081 499R ps RP1 18KI8 1 TO VALVES D oo 2 5 1 LS 0 2 or L3 7 2 21 LE Ov var i P2 5 5 6 3 o 5 POWER IN PBI0 4 6 5 4 4 15 v 7 24 PB lt lt 3 9 5 97 6 6 5 B O 6 B vi 0 4 S e 2 E 4 s 7 2 vor 1 3 5 os 08 9 2 PB3 PET 38 n 6 3 PEO 37 6 2 5 4 10 24 m VALVENF 7 07 lt com CGRID 10M 7 CGRID 6M 5R PBO 36 8 PBI 35 48 5 o 4 9 24 PB2 38 Le 5R 17 1200F 50V 2 m LL 35 25 us SR 18 2250 RP3 1 8K 18 g MAHTIA x TO STEPPER 30 ng 19 8 1 gt C23 22uF 35V 29 20 7 2 1 2 PCS gri s vv 3 3 4 k J 267 144 04 5 4 5 6 sus 018 5 2 H 2 Pct2 5 1 ore n 10 o TE ok 2 PCD 5 Bel 6 13 12 4 11 PHASEB 5 lt rs lt lt E PHASE 5 PB 6 6 8 E Ale DIR 5 oa 7 5 D 8
6. 8 7 6 5 4 3 2 1 YA 0 15 D YDI0 7 ust 35 43 2 GND lt Poo a H 861 YDS 4 Di 17 YA2 8 5 YD4 5 6 AN YD3 m os 5 YA4 40 a YD2 oe L4 YAS l es Wi YDI 5 YA6 41 be YDO 8 os 12 Yo YAT ei LAU Y YAS YA8 BRESET 3 BYALE YA9 34 YA9 2 25 YAS Te YATO a YATO 14 s 26 YATO YATI B a YATI 23 MH 15 27 YATI 2 2 M 2 2 d P33 INTI TRR as u 28 YA YAIS 26 YAIS 26 0 21 T 5 29 GND YAM 1 14 27 ism Hs m M c 26 a YA o 4998 15 20 teo YSWIO 1 P1 0 T2 20 amp E NRD 22 s JNPSEN e 22 5 8 Ha 082 JYPSEN 7 199 276512 P15 38 E q woo L3 mo lt 4 9 RXD we lt PLZ RXDO lt 80C320PLCC 5V 33 33 E GND lt inion Blometrica Inc 5 Medford Street Suite 101 MA 02143 Title Sorter Controller Stage Y Driver ize Document Number 350 5001 5 0 01 Date Tuesday April 29 2003 heet 10 of 10 8 7 6 5 4 3 2 1
7. 7 n s D 3 z T enno APPROVAL RIVERS ma S sac vaves LLesm o usr sisi vavo L ow XMOTOR PROCESSOR Y MOTOR PROCESSOR eris F swa 23 1 wa MULTIPLEXOR amp ADC m xpo moo on p o Lm woo o Fuss sus ADCSEL ES NICYPROC me PRESS2 YLED PRESS3 eS XLED FRESE PRESSE di sup sur mar as ass SORTING CONTROL nasi pud nasa dus ae ae sins suras sins LASERMON le usos ORE mast oer BLR GAIN amp INTEGRATION vamo sm maco Puni m JADCSTART mo ose moo ud m ANTEGRATE JNTEGRATE mume PRESSI m musst faGANSELY messa mcanse 2 sue messa
8. O N ass 1 300 1063 000 1 1 350 1022 000 ROTOR TOP LEVEL MATERIAL NONE APPLICATION WHERE USED FINISH NONE ADDITIONAL COMMENTS x WASTE PUMP ASSEMBLY PART NUMBER 2000 SCALE NONE DO SCALE THIS DRAWING SHEET 1 OF 1 ap sees ez x lt e mar per acus 7098 s ume wen naw ur 1770 a Revisen conPanen ________ e ano mask MOVE BACK COVER mes zx ooo om manas e missing ms ier ann Far sme wase NOTE ier ars o sss review wewmG was mmm fie mm 20s ee rs 2 sme 1 HOLE CHART 6X 266 THRU LI 4 438 X 260 N S 8X 48 38 UNC 2B THRU C 8X 206 08 375 X 5 D 2X 10 32 UNF 2B THRU E 2X 84 40 UNC 2B THRU F 1 4 20 UNC 2B X SOV G 10X 9 266 THRU LI 438 X 260 5 H 1X 6 606 THRU 9 375 X 250 V N S J 8 201 THRU 15X 6 32 UNC 2B THRU MASK 5 X 5 5 F S Me MASK 8 X 4 0 F S MASK 38 X 9 88 F S M4 MASK ANODIZE 5 X 17 89 6X 9 34 THRU 8X MIN RAD 00 3
9. 00 002 020010 0 0000000 27 Daily Maintenance 27 VasualT bing Che he etii ERU LU PUR 28 ANNUAL PREVENTATIVE MAINTENANCE PROCEDURE enn 29 SYSTEM INTERCONNECT DRAWINGS 31 GENERAL PARTS LIST aa e EA EEA a aT E SEA aR EN EaR 32 PRINTED CIRCUIT BOARDS 33 MAJOR CABLE ASSEMBLIBS uu 33 CONTACT INFORMATION NORTE ___ 34 NOTES 34 Union Biometrica Inc Confidential 2 www unionbio com Introduction The COPAS BIOSORT automates the analysis sorting and dispensing of objects ranging from 40 125 microns including particles beads seeds and small model organisms Using the physical parameters of object length optical density and the intensity of fluorescent markers objects are sorted according to user selectable criteria and then may be dispensed into stationary bulk receptacles or multi well microtiter plates This COPAS BIOSORT Service Manual details those procedures pertinent to the maintenance of the COPAS instrument and all top level assemblies that are used in the manufacture of the instrument Requests for more detailed lower level parts and materials will be addressed by Union
10. aux an m Eod m m st wore 6 sa wore icio AWOFF sONPUTO 3 ssa BONPUTO 3 soon moon NIONTG BLR GAIN amp NTEGRATIONO Pes 559 2 ii R2 INTEGRATE Hac 2 F act mume E s sins EL sc mar sins BINTSEL3 BINTSEL3 AUX2SIG AUGSIG wa NM_SPROC TM 8500 7 T RAWN BY nion Biometrica Inc NM_INTG2 5 Medford Street Suite 101 ATE somerville MA 02143 Sorter Controller Module Interconnects WE Document Number c 350 5001 SCO DI Tuesday Api 29 2003 Fret 7 s x D 3 2 1 5 BIOSORT PAGE 1 8 7 6 5 4 3 2 1 C21 0 47uF removed from 2 of U16 u12 Ts EXTINT BI s e X 20 T FLINT s2 U14
11. oly 4 YR m 5 o wa m ws a PE Id GND2 GND2 KD GND1 GND2 1 ARE as xd 3 TO DIGITAL s 4 d 2381 PANEL METER m has ora 7 gt veer LEO E TNI oW 2 7 m m w em con 5 DVM 5 gt CAL vec E v E 253904 ron 200k E ps enor 1 ow Ou SETGAIN vci ESI Curtis 701 RR 601 005120 2 BLK p R26 w 2 vx See ECO Note Hour Meter s 4 4 5 uw 5 E SHOWN FOR REFERENCE ONLY nor __ E 75 Om a 2 pn 2 os E vei P7 17805 E 1 TOFANS wa ia 82 veer 2 um 7 a GREEN E 75 33 2 ms he IMPORTANT FUSE NOTE E x x 10K me compliance requires that the correct fuse be used at 5 s H E F1 For 230V systems the correct amperage rating is GNDI RSID Ps 2 x 3 m gt 100 mA For 120V systems the correct amperage rating far DIS_OFF ENT Qu queue IRLDO24 is 200 mA In both cases the fuses must be Type T mis Slo Blo with appropriate
12. q s o q ooo Oo o o to 40 to 65 65 CD e 25 co x G Z 5o Co x 2215 5 4 0 15 UNION BIOMETRICA INC COPAS 5 D 55 0 5000 000 NNN g S VLL C 51 8 7 SENE m 54 a THIS DRAWING IS SOLE PROPERTY REV REVISIONS amp DESCRIPTION DATE MADE BY APPROVED UNION BIOMETRICA INC AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER THE CONTENTS ARE FOR THE PURPOSES ONLY 1 INITIAL WORKING DRAWING 06 12 02 OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC AND SHALLNOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC WHICHEVER COMES FIRST DIMENSIONS ARE IN INCHES PRAN MESSINA ME UNION
13. 02143 24 6 gt Sorter Controller Ext amp Flu1 Chans 33 33 33 33 33 33 33 33 33 33 33 33 Document Number lev GND 1 GND 1 350 5001 SCO D1 Date Tuesday April 29 2003 heet 4 of 10 8 7 6 4 3 2 1 COPAS BIOSORT PAGE 4 01048 5 lt 6 1 412 a FL2SIG m P 15 ax 20K 8104 PEE 5 U1018 BSDO gt AF42 4 5 06 8507 10 1 gt U103 BSD2 9 ay er 7 15 1N52468 BSD3 8 2 5 16 8504 7 x om M 8500 o gt UA 8505 6 BSD1 10 1 2 gt 100K 0105 GND 8506 5 a 8502 9 RH om 1 aur 8507 4 3 8503 8 2 07 GND GND D3 8504 eR ed and e n 0 E 5 BGAINSEL3 ud 8506 5 bs 9 nsw 8557 07 GND GND 7524 12 14 JEINTSES O c w A BSWR2 L 7524 INTEGRATE DUMP ADG417 E
14. COPAS BIOSORT PAGE 10 8 7 6 5 4 3 2 1 Rev ECNNO APPROVAL DATE REASON 4 EXT 3 33 3 2 R2 24V c _ 220 5 FEE 2 7 16 8 1 0 BS2 PLS amp 9 LASERMON NET 6 n 52 WAL _ 19 PRESS o1 15 2 S2 DIR 6 12 BT n PRESS2 2319 597 PB 1 m 12 PRESS3 5 2 Q 52 3 Been 52 WA2 n PRESS4 lt m 9 5 u 22 BS2_AWOFF 7 5 15 AUX1 JEN Es Aro Won 5 45V 19 52 WBl a O R2 17 AUX3 i 2 5 1 21 20K 20 V 3 MO
15. UNION BIOMETRICA A Harvard Bioscience Company COPAS BIOSORT INSTRUMENT Automated Analysis Sorting and Dispensing of small model organisms beads and particles SERVICE MANUAL Rev 1 00 Last Updated On May 8 2003 Table Contents TABLE OF CONTENTS a EN 2 INTRODUCTION uuu 3 WARRANTY d ce 3 WARNINGS AND PRECAUTIONS 4 SHIPPING CHECKLIST FORM 5 For Customers located in North America nne enne nre 5 For Customers located in Europe except United Kingdom sese 6 For Customers located in United 1 7 REAGENTS co e 8 Shear tieu de es Cleamim e us 8 s Sterilization Soliifi TER SEERE 8 MATERIALS REQUIRED FOR SERVICE NOT PROVIDED 9 WORK AREA AND FACILITIES SPECIFICATIONS enne nenne n naa 9 Work
16. MAIN INSTRUMENT MICROINCHES UI D 350 1002 000 BLACK ANODIZE SCALE 1 2 DO NOT SCALE THIS DRAWING SHEET 1 OF 1 UNION BIOMETRICA INC SOMERVILLE MA 617 54 705 1252 ASSEMBLY FINAL OP E THIS DRAWING SOLE PROPERTY UNION BIOMETRICA INC AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER THE CONTENTS ARE FOR THE PURPOSES ONLY OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC AND SHALLNOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC WHICHEVER COMES FIRST REV REVISIONS amp DESCRIPTION DATE MADE BY APPROVED INITIAL WORKING DRAWING 04 22 03 J M M TERED em wem UNION BIOMETRICA INC TOLERANCE ON ENGINEER SOMERVILLE 617 591 1211 DECIMALS FRAC ANGLES TITLE O APPROVED 005 PROJECT QUALITY ASSEMBLY MES ALL FINISH SURFACES TO 32 ADDITIONAL COMMENTS SYSTEM ELECTRONICS PANEL MICRO INCHES UNLESS NOTED X MATERIAL NONE SIZE PART NUMBER REV 350 5000 000
17. V m EN 4 0 5 PB S 7 B 5 7 19 cv 15 71 53 m 10 0K us CGRID 10M U10F R3 499K lt t lt Cx 1 x O ts BLIMSW LMSW 2 Vsetpoint 1 RI6 45 t ladj UI0A 499K 20 0K xi N 10 PB6 PB7 2 ISPOWERED 2 1 R14 R100 LIMSW D fo fo 499K gt T 2 23 2378 e e 1004 mz 022 50V PEE 433 a ja 1B462H V 433 21 __ cu 5R 5 V a 5R 5R 5R 022 50V 022 50V 22uF 35V i a Vsetpoint 170mV c30 Ca V 340mA winding 5R oT P3 5 ci 4 04 1 Thou 12 13 i 5 tat 4 CAVRO PUMPS 10 7 g Tin Hg 6 Rlout 7 33 JP100 S 5 PB3 a 2 PB2 34 CGRID 8M e Y R106 R107 ALT FUNC lt 5R 33 022 50V 0 22 50V 0 22 50V gt 499R 499R P100 ASH U 1 LTC1386CS MAX3232 5R 5R ho gan 3 P101 CGRID AM 5R 4 1 0 i s 2p MNTI MNI3 MNT4 058 8103 478 6 0 7 4 Dp 6 6 6 IB462H 478 8 SFA A CGRID 8M 5R litle a 47R gt a ReFlx Sampler Board Peripheral Drivers DO NOT POPULATE Document Number lev 5R B 340 5005 SCO B1 pate Tuesday April 29 2003 heet 1 of 2 5 4 3 2 1 REFLEX SAMPLER OPTIONAL MODULE PAGE 1
18. X BLUE 20 5 X RED 8 5 BS1_PLS DA X GREEN 21 BS1_AWOFF X BLACK 9 BS2 PLS lt X WHITE 22 m y BS2_AWOFF S1_DIR X_YELLOW 10 RXD 1 10 853 PLS Y_BLUE 23 TO BULKHEAD DB9 BS3_AWOFF RED GND lt q 3 15 5 5 5 Y_GREEN 24 lt DINPUT3 P mm Qe m 5 Y_WHITE 25 1 X PLS Y YELLOW 13 16 X PLS 1 BS1_PLS 17 X_CW CCW m 2 2 2 2 18 X_AWOFF X CW CCW 3 Y_cw ccw 3 S1 DIR DES E x 19 4 4 4 C 20 lt X 5 5 BS1_AWOFF E XLOLIM 2 d 2 KHU GND lt 7 GND lt 7 GND lt 7 A ANLOLIM Shes 8 l a 24 GND lt 9 GND lt 9 GND lt ai 7 4 7 4 25 Mv 10 V RE MVC 8 26 Y Pis X RED B Y RED 51 BLU 12 27 X GREEN Y GREEN 15 51 RED Y_Cw ccw X_BLACK Y_BLACK 5 GRN 44 PRAWN BY Inion Biometrica Inc 2 Y_AWOFF X WHITE is i S1_BLK 5 Medford Street 30 X YELLOW Y YELLOW 2 51 MA 02143 31 lt DINPUT2 16 i 51 YEL Pate 2 Ed 25 HDR16 HDR16 HDR16 34 Bulkhead Connector Detectors amp Steppers CON34 V ize Document Number GND 8 350 5002 SCO A3 Date Tuesday April 29 2003 heet 1 of 2 8 7 6 5 4 3 2 1 COPAS BIOSORT BULKHEAD CONNECTOR PAGE 1
19. gt wese AUX3 151 152 gt 33 GND E gt 0 0 255 299 11 7 mV per level or 43 V of HV per level 0 0 255 299 11 7 mV per level 43 V of HV per level En GND 6177 33 520 536 R151 amp R153 deleted at 0150 inputs Annotations corrected R159 amp R160 ref designations uncrossed R157 and R160 were 7 5 Rev D R159 amp R160 again uncrossed 5 R158 499K gt RS104A U152 2 in lt 3 8 Fout 100K 1 6 et Rs104C Thrsh gt 2 5 gt 8 RS1048 LM331 100K E 100K gt R157 001 200 GND cde 33 J GND V GND GND 5 R160 499K RS105A vis 2 n lt 3 Fout 100K 1 n ss pis 6 lout RS105C 1 Thrsh NE 2 2 l gt ref 165 1 cies 51058 LM331 100K 100K 1 RIS9 1 45 GND 200 GND V GND 33 GND V GND AV 15v o p 33 33 inion Biometrica Inc 7 GND GND 5 Medford Street Suite 101 02143 Title Sorter Controller Misc Buffers amp VFCs ize Document Number 350 5001 5 0 01 Date Tuesday April 29 2003 heet of 10 COPAS BIOSORT PAGE 9
20. p COR 3 36 VALVES Inion Biometrica Inc 2 ow 538 xcn2 4 9 5 Medford Street O 15v 54 548 c o 5 5 ssa s 8 1 omerville 02143 O S VALVETZ i ai VALVEIS i c VALVET4 4 Bulkhead Connector Valves amp Red Laser CONS x VALVETS i VALVEI Document Number P 350 5002 SCO A3 Date Tuesday April 29 2003 heet 2 of 2 COPAS BIOSORT BULKHEAD CONNECTOR PAGE 2 8 6 5 4 3 2 1 Rv _ APPROVAL DATE lt i 33pf 5 4 XCTL2 100K 15 xcu wi 6 SMB JACK 2 2 2 GND gt 21 AD8400AR1 u N JUMPER ie R2 499 y 6 8 4 5 DIN T gt n t ADS 1 5 499K DIN EE 357K x Di Xf 3 2 Hamamatsu 7510 e 4 s y jv RIS 1 499K TS AD8131 gt 11145758 a RI a x J 8 2 2 10K 5 11 pz RB 499 5 13 C 4 2 17145758 v V V JUMPER 15
21. o1 o oi 1 853 PLS ii A 2 853 AWOFF 11 L C82 8 3 GND 4 2 o o 7 X Pis 1 16 GND CON4 X i 7 bi X AWOFF gt RSI DINPUTI s 47K ps 20 1NS711 XLOLIM 2 2 A THOM VALVES N 20 25 5 1 2 RXD 18 12 ain gt RxD0 m is Y_cwiccw 1 5 29 TxD 14 DINPUT2 5 SALE 31 T20UT lt 52 C58 P RST gt BRESET 33 lt p 34 71 wr 45V 33 33 TPS TP6 TP8 CON34 6 2 c d 051232 EN V GND 202 C56 V V 33 33 u54 GND GND GND GND 8 5 c m L gt m inion Biometrica Inc c54 cio JK 7 5 Medford Street Suite 101 20MHZ OSC 33 33 33 33 33 100 100 somerville MA 02143 4 Title Sorter Controller Misc ize Document Number B 350 5001 SCO D1 Date Wednesday September 11 2002 heet 6 of 10 8 7 6 5 4 3 2 1 COPAS BIOSORT PAGE 6
22. o Argon Laser System 100 120V AC 20 Amp 50 60 HZ single phase on a dedicated line with a protective earth ground o Air Compressor optional 100 120V AC 13 Amp 50 60 HZ single phase on a dedicated line with protective earth ground May be placed on instrument line if necessary if surge protection is provided for the instrument PC and monitor e Circuit Requirements for 230 VAC Countries o COPAS Instrument PC amp monitor 220 240V AC 10 Amp 50 60 HZ on separate dedicated lines with protective earth ground o Laser 220 240V AC 16 Amp 50 60 HZ on separate dedicated lines with protective earth ground o Air Compressor optional 220 240VAC 10 Amp 50 60 HZ single phase on a dedicated line with protective earth ground May be placed on instrument line if necessary if surge protection is provided for the instrument PC and monitor Union Biometrica Inc Confidential 10 www unionbio com Unpacking the COPAS Instrument General recommended guidelines for unpacking a COPAS instrument and laser assembly 1 Confirm work area and facilities meet specifications outlined by Union Biometrica in this Service Manual Inspect all containers for damage If there is any damage contact Union Biometrica immediately to report the damage Inventory all containers It is recommended that if storage area is available customers keep the COPAS instrument containers to be used for long term instrument storage o
23. 2o 24 8 1 Vout wwe Gs Gs p 37 c39 2 22 22 22 20 GND L 7 GND lt 78L12 sm VA vR7 1 2 1 E Vin Vout vn e in 7 Via 5 GND 79L12 sm ca p 22 22 22 m 2 Union Biometrica Inc 35 Medford Street x Somerville 02143 Title PMT Preamp HV Flu2 PMT Document Number lev E 350 5009 SCO A1 Date Wednesday April 30 2003 heet 6 of COPAS BIOSORT PMT PAGE 6 R81 1K 3 PMT3 10 0 DY8 DY7 DY6 DYS DY4 DY3 DY2 DY1 0 DY7 DY6 DYs DY4 DY3 DY2 R88 332K R89 332K R90 332K R91 332K R92 332K R93 332K R94 332K R95 332K R96 332K R97 332K 91 650 m 17145758 68pf cas 7 100K AS v R87 15711 U12A 8 17145758 V be WB 3 o 2 22 22 22 2 c p p ce m 22 22 22 2 R85 10K J4 SMB J 41 JP5 JUMPER 13 ull DIN OUT R82 4 99 OUT voc 4 O R86 4 99 AD8131 R107 49 9 gt FL3 gt FL3 R108 49 9 J
24. 335 5064 000 300 5055 000 Blaster Valve 300 5048 000 Valve Assy s 310 5018 000 STAGE ASSEMBLY INCLUDES 300 5019 000 C A 350 5008 001 EXTINCTION DETECTOR COPAS BIOSORT SYSTEM INTERCONNECT INTERNAL UNION BIOMETRICA INC LAST UPDATED 1 21 03 Motor 2 Motor 3 Reflex Power Aux 25 pin J18 pin J16 pin pin J15 Mixers Sample Valve J14 EXT Laser Diode Laser Stage 15 pin Valve Panel PMT Assembly COPAS BIOSORT CONNECTOR PANEL FRONT VIEW HOUSE AIR DIVERTER OM MANIFOLD REGULATOR MAIN INPUT REGULATOR SAMPLE PRESSURE SAMPLE CLEAN BOTTLE SAMPLE REGULATOR BOTILE 2 LITER SAMPLE CUP AIR VALVE CLEAN BOTTLE REGULATOR FLOW CELL SOLUTION DIVERTER AIR VALVE VALVE DIVERTER VALVE UNION BIOMETRICA INC SOMERVILLE MA 617 547 7703 PNEUMATIC DIAGRAM COPAS 550 5000 General Parts List The following general parts list includes all assemblies that comprise the COPAS BIOSORT part number 350 5000 000 For a specific bill of material for an assembly please contact Union Biometrica Inc directly Part No 350 5000 000 300 5036 000 300 5037 000 300 5059 000 300 5061 000 300 5065 000 350 1001 000 350 1002 000 35
25. 9 PME JINTSEL3 5 6 Raz 24926 75RD 32 3 7NTSELA 5 15 BITS 4 2 DUMP gt pump sro XIRQ Tm BINTSELA 5 T vino 5 5 gt 6 Hi TRE omm 6 Ye ocu 7 5 8 TRCH1 5 s v 8 sawa m Ls Ya RS7 ACQUIRE M C GRID 8P 100RSIP6 BC eo SORT 16 and R161 can be pinswapped 74HC541 and restructured as required A inion Biometrica Inc SA 0 2 is sao 5 Medford Street Suite 101 SAT 02143 5 2 cei c65 c66 c67 ces cio ean te ae D Sorter Controller Sorter Processor 33 33 33 33 33 33 33 33 33 33 33 33 33 580 WEE ize Document Number ev 350 5001 SCO D1 Pate Tuesday April 29 2003 heet 7 of 10 8 7 6 5 4 3 2 1 COPAS BIOSORT PAGE 7 D XDI0 71 5 2 GND lt Poo Ho a 5 204 e Hs XD5 4 bi EE 17 XA5 8 D 20MHZ XD4 ERES 16 T A b 6 o Q 3s KAS 4 5 5 25 X2 7 pa 95 Da x 5 5 c
26. Di oum LL 77 LF412 FL2 8502 9 mE 1 52468 8503 8 2 7 15 3 8504 7 Qui 5 ne 16 V BSD5 6 BSD0 n gt uA 2 GND BSD6 5 BSD1 20 1 R2 100K us 1 BSD3 8 2 3 n PRESS2 L gt TN D3 our LF412 12 14 BSD4 7 12 PRESS3 BGAINSEL 6 1 412 PRESS4 L gt wr p SEDE 2 x 8507 4 3 15 AUXI D7 GND gt GND 06417 o 16 AUX2 n M AN lt 7 AUXS BINTSEL1 gt 9 1 BSWR1 1 2 At INTEGRATE C gt 21 22 sv JDUMP 32171 24 2b ADG417 26 4 asv M CON26 5 Lal ao 7 6 LF412 GND x gt FLISIG u7 15 ca 15pf 12V RB 20K R9 10 0K t RE ws 06 ae BSDO gt AF42 05 BSD1 10 1 us Di oun BSD2 9 7 15 ci 15pf D2 REF 1452468 oun 2 5 ws LS BSD4 A 8500 LS em T dk V BSDS 6 BSD1 10 1 R7 100 GND 8506 5 5 8502 9 ar eum D s gt ruin BSD7 4 3 BSD3 8 2 D7 GND GND D3 our 8504 T RS2 21 6 15 y BSDS LF412 P 10 BGAINSELI ONSE BO w 8506 5 9 BGAINSEL2 BSWR1 4 BSD7 357 NS ADGA417 8 BSWRI 7524 3 7 BINTSELI ud vig 44 4 q 6 BINTSEL2 w 5 BSWR1 AD7524 3 2 DUMP s INTEGRATE gt 6417 5 0 7 inion Biometrica Inc l v A 5 Medford Street Suite 101
27. 110 Install COPAS System Software using Installation Disk Install Source Disk and open Key Maker Enter System ID number and options The system will issue a new registration code Highlight and Ctrl C the new registration code Open the system software Open About MC menu and select Registration Place the cursor in the registration code box and hit Ctrl V Ensure the new registration code has been inserted Verify the System ID number matches the number in Key Maker Right click on the COPAS icon Select Property select Find Target and Select mc ini Verify that the correct values are set in the ini file according to the attached examples Open the file menu and select save Perform the following set up on computer Initialize Network Port Enable Select System Printer Install WINMDI POWERWARE Registration Code and Communications Port Software from UnioBio system folder gt B 3 Argon Laser a Plug Argon Laser into correct voltage b Move yellow wire on panel meter to top right hand position 2 decimal points of connector Turn ON AC power d Verify 2 decimal points on LCD e Power up the computer and open the program by double clicking on the COPAS Icon Open TOOLS Menu and select Run External Laser Turn laser on in Run Mode f Setlaser power to 10 2 mW on the laser power supply by placing a laser meter set to 488nm between the laser and cylindrical lens and adjusting th
28. 38 33 33 33 33 33 C92 REX cor GND lt 0158 33 33 10 amp 33 R2 ama 5 ax LASERMON 7 LMSIG 10 0K 6 39 5 es c 176 GND GND 33 5 33 33 33 33 33 2 79105 79 12 GND 4 4 P 1 GND 2 3 3 nm O ovr N o 5 P 2 LF412 inion Biometrica inc REF 3 C94 e r Cos 5 Medford Street Suite 101 38 10 33 omerville 02143 Title Sorter Controller ADC Subsystem Document Number GND GND 350 5001 SCO 01 Date Tuesday April 29 2003 heet 2 of 10 8 7 6 5 4 3 2 1 COPAS BIOSORT PAGE 2 8 7 6 5 4 3 2 1 R97 was 7 5K 92 parallel to C110 deleted HV1 Grn HV2 Yel HV3 Red R83 20K Annotations corrected and added U71 pin 1 was 5 5A 0 2 R82 vou 8 snm Al VoU 5 gt THRESHOLD 3 ea x vous GND lt mv DACLD s 255 120V 5000 71 m P 47mV
29. APPLICATION FINISH C 350 5027 000 WHERE SED NONE SCALE 1 2 DO NOT SCALE THIS DRAWING SHEET 1 OF 1 THIS DRAWING IS SOLE PROPERTY UNION BIOMETRICA INC AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER THE CONTENTS ARE FOR THE PURPOSES OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC AND SHALL NOT BE USED ASA BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC WHICHEVER COMES FIRST REV REVISIONS amp DESCRIPTION DATE BY APPROVED 1 INITIAL WORKING DRAWING 07 24 02 J M M UNLESS OTHERWISE SPECIFIED DIMENSIONS ARE IN INCHES TOLERANCE ON DECIMALS FRAC ANGLES 010 4 XXX 005 560 5000 000 ALL FINISH SURFACES TO BE 32 MICROINCHES UNLESS NOTED ITEM NO QTY PART NO DESCRIPTION 1 300 1042 000 RING SAMPLE BOTTLE SUPPORT 2 1 350 1003 000 PLATE SAMPLE BOTTLE 3 2 300 1044 000 BRACKET SUPPORT 550 5000 000 APPLICATION WHERE USED MATERIAL NONE FINISH NONE DRAWN J MESSINA 07 24 02 UNION BIOMETRICA INC PAINEET s SOMERVILLE 617 547 7703 APPROVED TITLE m QUALITY SUPPORT ASSEMBLY ENG ASSUR ADDITIONAL COMMENTS SAMPLE BOTTLE SIZE PART NUMBE
30. Add 40ml of COPAS Cleaning Solution to the primary sample cup and cap firmly Select Sample Valve for approximately 5 minutes Rinse primary sample cup with distilled water twice Fill primary sample cup with distilled water and cap firmly Select Sample Valve for approximately 5 minutes Fill sample with distilled water and close cap Close software and turn off power to system and components Union Biometrica Inc Confidential www unionbio com 25 Instrument Test Worksheets Sort Delay 1 2 3 4 10 11 12 Value A B C D E F G H 1 2 3 4 10 11 12 A B C D E F G H 1 2 3 4 10 11 12 A B Union Biometrica Inc Confidential www unionbio com Sort Delay Date Technician 1 Well Date Technician 5 Well Date Technician 26 Daily Short Term COPAS Instrument Maintenance The following Daily Maintenance procedure also found in the Operator s Manual should be performed by the Operator at least once daily when the instrument in use If the COPAS BIOSORT will be idle for one to two weeks follow the short term maintenance schedule outlined in the Operator s Manual If the COPAS BIOSORT will be idle for greater than two weeks it is recommended that the short term maintenance schedule also be followed The following m
31. BDINPUTS aa Ps AEP STXDO 7 GA 5 5 H PLT Ro gt SALE 8501 7 BDINPUT O 3 u67 Sp 2 18 _ BSDO 54 15 EES 41 uo 502 4 42 Y 6 8552 X 5 BSD3 s 6 Yt a BSD4 ss 7 Ys 1 s 6 6 2 BSD6 sy 9 n OB 7 1 m i 1 apcstaRT gt ADCSTART SAN ADGE ADCSEL ed SAI2 ADCHBEN ADCHBEN EVE 74HC541 3 ADCCHO sao 4 21 14 ADCCHO CLK MHZ s d AEn 5 wa Siz De BLAST BGAINSELI 6 ADCCHS SATB 37 JGAINSELZ 3 17 apa ADCCH3 E 557 9A BOOST BOOST Am Ha v Hs BGAINSEL2 501 500 62 BENABLE 5415 26 5 Im BIS qs cB mmu 503 27 26 GAINSEL2 5 7 E 8SWR1 gp 457 503 GANSE2 ADCCH O 3 45 vaven D gt cu 507 GAMES 055 GAINSEL4 501 TE 500 4 5 X m 35 GANSEA 7 Sm D n nPAcup 8 Ye 7 7SWR NI JINTSEL2 503 39 2502 BENABLE 1 75RD 580 WR 27 INTSELS 504 190 s erm Hiep INTSELA 505 17 Kos 15 E T pe SD6 40 9 14 507 Res Mera Dm YO 57 THRESHOLD D MCOATHRESH 5 soo D 856 u69 100RSIP6 13 31 TOF dual 19 GAINSEL3 2 18 3 gt T 2 Ds 4 Daama ANTEGRATE 20MHZ gt ax gt 90 ae 3 n M v mE 1 3 4 5 PEE 588
32. CLKOUT 34 46 SLRD SR 3M 20 CLKOUT 4 13 WAKEUP ut A 33 5 12 WAKEUP V 101 9 H2 6 5R Dd 96 BINFLAG 5R 7 10 433 433 6 97 P 74 3 P r d 5 SDA 2 PB g o PG 8 N i 7 PDS 11 x 19 9 PDs V o 2992222229222 9 y G8 m m 5 2999990909222 2 298 02 5R RIB RIS 2 2282555552358 9 24 14 15 9 1K 1K SLRD 6 5 PDT 433 sao 1 8 SWR 18 17 PDO omes 2 19 5 glelely 3 tt Eg 1 16 POWER sa T gt sR 2 15 Ps SDA 3 T4 SLWR R21 NDA XCLKSEL 5R 3M 20 4 1 24LC00 V 5 AAA 12 DISCONF 390R 5R 5R 5R 5 nom 05 01 7 10 EA EA DISCON PC2 NE must be pulldowns gt nee ReFlx Sampler Board USB Control 10kn6 ize Document Number 340 5005 SCO B1 Date Tuesday April 29 2003 heet 2 of 2 5 4 3 2 1 REFLEX SAMPLER OPTIONAL MODULE PAGE 2
33. FS input s3 D x Hase 22 S range range ignored um PIB 26 EXTSIG 2 24 FLISIG 5 U13B P3B 25 500 7 FL2SIG 6 40826 26 x 7135 E 5 np 12 d 7 THRESHSIG D AUX3SIG 10 E 9 10 FLINT 2 5 di DBO 5 5 FL3INT 71 512 DB1 TRCH O 1 ss x FL2SIG 53 REF 19 DB2 TRCHO 1 FL3SIG s 514 REF DB3 TRCHT 16 AO 3 AUXISIG 55 48 DB4 B ELMO AUX2SIG s GND lt _ AGND 085 i 086 1 2 A3 087 Np A2 ADCSEL q s 12 23 Al GND GND ADCSTART g CONV T 7 R25 1580 5 pos vss AN ADCHBEN gt 5 EN 20 Busy vss O ADCCH O 3 ADGADG GND lt 128 12 n sva DGND gt GND ad 18 E ax AD7876 LF444 85106 04 7 P4 3 FL3SIG 7 8 d 1 au TR FSG 5 6 x 2 FLISIG 3 4 5 c38 3 EXTSIG 1 2 33 2 4 1 TOOR ax GND RSS 1 2 PRESS1 3 PRESS2 A via PRESS3 7 as R21 1 0K PRESS4 gt THRESHOLD 2 12 17444 14 2 P3 5 THRESHSIG 822 gt MCOATHRESH 13 zi 7 P3B Ge m 6 2555 c37 a 33 o 33 E ET gt AF44 GND P 10 GND 5 8 PIB B c35 w vie OC 78105 LM341 12 3 1 1 3 e eus 179 i Oe N 5VA N oa V n u GND 2 2 m A
34. Inspected by Part Number 066 0007 000 066 0007 100 066 0007 200 066 0007 300 066 0007 400 066 0009 010 066 0009 020 100 0010 001 111 0250 002 119 0003 000 145 0020 000 300 5044 000 300 5062 000 300 5068 000 300 5070 000 300 5072 000 300 5080 310 5018 000 310 5071 000 340 5014 000 350 5074 000 Date For Customers located United Kingdom Part Number Quantity 1 N e hL Packaged by 350 5000 000 COPAS BIOSORT Description Power Strip Jumper Cables Power Strip Laser Wall Cable Laser Compressor Wall Cable RS 232 Data Cable CSM Cable ReFLX ONLY Laser Power Cord Tubing Clamp PVC Air Tubing Threaded Barbed Fitting Slotted Screwdriver Waste Bottle Vent Tubing Waste Pump Tubing GP Sheath Reagent and MSDS Cleaning Reagent and MSDS Installation Disc Laser System Cable GP Control Particles and MSDS Bubble Trap Filter only with ReFLx Reference Manual Information Packet Union Biometrica Inc Confidential www unionbio com Serial Number Date Inspected by Part Number 066 0007 000 066 0007 100 066 0007 310 066 0007 410 066 0009 010 066 0009 020 067 0020 003 100 0010 001 111 0250 002 119 0003 000 145 0020 000 300 5044 000 300 5062 000 300 5068 000 300 5070 000 300 5072 000 300 5080 310 5018 000 310 5071 000 340 6000 000 350 5
35. Prior to Installation of the COPAS BIOSORT instrument confirm that the work area and facilities comply with the below specifications Work Area Requirements e The COPAS system requires a permanent open level vibration free working space with the dimensions of 3 feet 1 meter deep x 7 feet 2 3 meters wide x 4 75 feet 1 4 meters high No other equipment should be placed over the COPAS system There also should be user access on both sides of the working area e ACOPAS system with the optional Zymark Twister Robot will need an additional space to the left of the COPAS of 3 feet 1 meter deep x 2 feet 0 6 meters wide x 2 feet 0 6 meters high e The Union Biometrica supplied optional Air Compressor requires approximately 14 inches 36 centimeters deep x 18 inches 46 centimeters wide x 20 inches 51 centimeters high of stable floor space Environmental Requirements e This instrument is designed for use at an altitude of up to 2000 meters in an ambient operating temperature between 15 and 30 C 60 to 85 F with a relative humidity of 0 to 85 non condensing decreasingly linearly to 50 relative humidity at 40 C 104 F Temperature should not fluctuate more than 1 5 C from the time of experimental setup through completion or adjustments will need to be made e Ensure that adequate ventilation of the system components is provided It is important that ventilation openings not be blocked while the system is powered
36. 16 www unionbio com Waste Alignment 1 Turn the waste tray adjuster screw counterclockwise and push the tray toward the right as far as it will go 2 Turn the sheath valve ON and Sorter Valve OFF 3 Turn the screw clockwise until a stream of sheath just flows through the opening Ensure the stream is flowing straight 4 Turn the Sorter Valve ON and check that no dripping occurs 5 Verify that the stream hits the waste tray just after the bend in the metal plate Stage Alignment 1 Open TOOLS menu and select Align Stage 2 Placea 96 well plate onto the stage 3 Click the WELL button and then click the TEST DROP button to verify that the test drop has been deposited neatly into the correct well 4 Adjust the alignment by changing the OUT IN gt and LEFT RIGHT gt positions by increasing or decreasing amount of steps if the stage positioning is incorrect This may be done using the arrow buttons next to each of these fields Repeat the TEST DROP procedure until the drop falls in the center of the well 5 Click the NEXT COL or NEXT ROW buttons to allow for additional positions to be checked Continue through at least of the first row of the 96 well plate 6 Click the LAST WELL button and then click the TEST DROP button again to verify correct stage alignment 7 Return to WELL AT and verify drop is still in center of well 8 Repeat the above alignment procedure using LOAD B and WELL for align
37. 22 GND R59 94 78L12 sm 332K v v DY2 2 Vin Vout R60 3 332K 7 DY1 Vin 5 GND 861 79L12 sm 332K Ble detis T oe 22 22 22 22 Union Biometrica Inc 35 Medford Street Somerville MA 02143 Title PMT Preamp HV ize Document Number lev E 350 5009 SCO A1 Date Wednesday April 30 2003 heet of COPAS BIOSORT PMT PAGE 5 PMT2 10 DYS DY8 DY7 DY6 DY5 DY4 DY3 DY2 DYI 0 DY7 DY6 DYs DY4 DY3 DY2 RO 21 332K R 332K R72 332K R73 332K R74 332K R75 332K R76 332K R77 332K R78 332K R79 332K c32 c33 c34 HV2 SMB Ji Y 1 10pf gt R62 100K 2 Y V2 n JUMPER S 2 BEP R63 N 8 4 t DIN e R64 1 5 499K DIN 3 2 m gw VOCM 4 R67 6 10k R68 499K Uk AD8131 17145758 8 RI 499 gt FL2 17145758 H lt gt 816 499 2 JPA
38. 5 CN 5 6 1 412 a 4 FL3SIG ur gt LEN 122 ax 8108 20K pet 16 D TP102 8500 cee 15pf 1412 2 07 8507 10 1 N U108 BSD2 9 kh M 7 15 i 8503 8 2 5 16 1852468 D3 our M RFB BSD4 7 8500 V 8505 04 BSD1 1 2 8107 10 0K GND BSD6 5 amp BSD2 9 a SM 1 8 t e gt aint EM 5 o evo GND SUN ow 2 BSD4 apes 6 s E 5505 are L412 BGAINSEL4 13 8506 5 lt 2 8507 4 3 07 GND GND 7524 12 14 6 85102 10 _ BGAINSELS BSWR2 S 5 SGANSELA AD7524 7 BNE 6 BINTSEL4 5 4 ADG417 3 2 INTEGRATE 1 I 47K 8500 7 inion Biometrica Inc 2 Xv ag 5 Medford Street Suite 101 02143 cian C133 c139 ca 143 145 C134 6138 m S C 102 amp Ch 33 33 33 33 33 33 33 33 33 33 33 33 orter Controller Flu2 amp Flu3 Chans ize Document Number GND lt GND 350 5001 5 0 01 Date Tuesday April 29 2003 heet 5 of 10 COPAS BIOSORT PAGE 5
39. 500 JEEP E TYP 220 TYP l 405 600 DETAIL esas fesnem UNION BIDMETRICA INC SOMERVILLE 617 547 7703 84 32 MICRUINCHES UNLESS NOTED MATERIAL 172 THK ALUMINUM d D 350 1001 000 13 __ APPLICATIDN SCALE 11 1 DO SCALE THIS DRAWING SHEET 1 OF 2 REVISIONS amp DESCRIPTION INITIAL WORKING DRAWING MOVE STAGE HOLES 1 50 12 20 01 ADD HOLES FOR BACK COVER 02 02 01 REVISED COMPARED TO ACTUAL 02 13 01 UPDATE PER MARK UP G M 03 01 01 CHG SUPPORT HOLE PATTERN TO 3 15 03 06 01 ADD FOR 05 25 01 55 05 28 01 ADD FAR SIDE NOTE 05 14 01 REVIEW DRAWING CHANGES 07 10 01 CORP WHICHEVER IS FIRST 4X PRESS 9 25 X 1 00 LG SST PIN FLUSH TO FAR SIDE MARKED C 2X 1 X 45 CHAMFER Slololviolalalulw 3 11X 9 266 THRU CBORE 9 438 X 260 DP 1789 16 500 8X 10 32 2 THRU 15 69 gt 4 6 249 THRU 1X 6 52 0 THRU 2X 8 32 UNC X 50 DP BOTTOM M1 MASK ANODIZE 5 X 6 0 FAR SIDE M2 MASK ANODIZE 5 X 17 89 FAR SIDE M3 MASK ANODIZE ON SURFACE INDICATED UNLESS OTHERWISE SPECITED DRAWN T FMULUNS UNION BIOMETRICA INC SOMERVILLE MA 617 547 7703 5
40. BIOMETRICA INC TOLERANCE ON ENGINEER KASSEY 06 12 002 SOMERVILLE 617 547 7703 DECIMALS FRAC ANGLES o per ITEM PART NO DESCRIPTION 005 PROEST T a AIR REGULATOR 2 1 119 0002 001 FITTING THREADED ELBOW REMOVE BURRS amp SHARP EDGES DUET FILTER ASSEMBLY ALL FINISH SU I 1 119 0003 000 FITING THREADED BARB ALL FINISH SURFACES TO BE 32 3 1 140 0002 000 FILTER REGULATER AIR PRESSURE EET 4 1 140 0002 001 BRACKET AIR FILTER NONE FSI AMETS 5 TOP LEVEL APPLICATION FINISH C 500 5056 000 1 WHERE USED NONE SCALE NONE DO NOT SCALE THIS DRAWINQ SHEET 1 OF 1 40 0002 002 PANEL NUT AIR FILTER THIS DRAWING IS SOLE PROPERTY UNION BIOMETRICA INC AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER THE CONTENTS ARE FOR THE PURPOSES OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC AND SHALL NOT BE USED ASA BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC WHICHEVER COMES FIRST REV REVISIONS amp DESCRIPTION DATE BY APPROVED INITIAL WORKING DRAWING 04 24 03 J M M UNLESS OTHERWISE SPECIFIED DIMENSIONS ARE IN INCHES DRAWN _ J MESSINA 04 24 03 UNION BIOMETRICA INC TOLERANCE ON ENGINE
41. Biometrica Inc on an individual basis Warranty The Union Biometrica COPAS instrument is warranted to be free from defects in material and workmanship for a period of one 1 year from the date of installation and acceptance Union Biometrica or authorized personnel will repair at our election by replacement any part of the instrument which fails within the warranty period As a part of this warranty period the customer will receive unlimited telephone assistance support Monday through Friday during normal working hours from 9 00 AM 5 00 PM Eastern Standard Time up to four 4 emergency on site visits per year all replacement parts labor and travel and one 1 preventive maintenance visit per year This warranty is extended to the original buyer purchasing the equipment from Union Biometrica or authorized distributor This warranty does not include routine cleaning adjustment or normal wear and tear from use unless designated by buyer as its annual preventative maintenance visit and excludes A Service or parts required due to damage caused by accident neglect breakage misuse alteration to the equipment electrical current fluctuations unfavorable environmental conditions work performed by an unauthorized agent or forces of nature B work which in the sole exclusive opinion of Union Biometrica is impractical to perform because of the platform s location or proximity to any other device C modifications to the instrument f
42. DATE MADE BY APPROVED C INITIAL WORKING DRAWING 06 03 02 ITEM QTY PARTNO DESCRIPTION 8 2 300 1001 000 BRACKET LEFT SIDE MOUNTING 9 2 300 1002 000 BRACKET RIGHT SIDE MOUNTING 10 2 300 1003 000 ROD Y STAGE TRAVERSE 11 2 300 1004 000 ROD Y STAGE TRAVERSE 12 1 300 1005 000 BRACKET Y STAGE TOP MOUNTING 13 1 300 1006 000 BRACKET X STAGE TOP MOUNTING 14 2 300 1002 000 BRACKET MOTOR MOUNTING 16 4 300 1011 000 BEARING LARGE MOTOR MOUNTING 17 4 300 1012 000 BEARING SMALL MOTOR MOUNTING 18 4 300 1013 000 VANE HALL EFFECT 19 1 300 1014 000 BASEPLATE X STAGE 20 1 300 1046 000 COVER X STAGE CONNECTOR 32 2 300 1100 000 PLATE LOCATOR 51 1 300 1103 000 COVER PCB X Y STAGE 2 4 300 5011 000 ASSEMBLY PCB LIMIT SENSOR 31 1 300 5024 000 ASSEMBLY MOTOR Y STAGE 35 300 5025 000 ASSEMBLY MOTOR X STAGE 6 UNLESS OTHERWISE SPECIFIED DRAWN J MESS 5 2 DIMENSIONS ARE IN INCHES MESSINA 06 03 02 UNION BIOMETRICA INC TOLERANCE ON ENGINEER KASSEY 06 03 02 SOMERVILLE MA 617 547 7703 DECIMALS FRAC ANGLES i XX 00000 APPROVED ITLE 005 PROJEC T QUALITY CONC ENTRICITY 005 TIR i ENGR ASSUR X Y STAGE REMOVE BURRS amp SHARP EDGES ALL FINISH SURFACES TO 22 COMMENTS ASSEMBLY MICRO INCHES UN
43. Ifconcentric rings multiple patterns or any other distortions are still observed the fiber is damaged and a new fiber must be installed 4 Cleaning Procedure Set the laser to STAND BY mode Close laser shutter Soak a piece of lens tissues with 100 methanol Clean the fiber face with soaked lens tissue Dry the fiber face with a second piece of lens tissue Open the laser shutter Perform appropriate Inspection pre installation or installation If a uniform circular beam is observed return to appropriate step If concentric rings multiple patterns or any other distortions are still observed perform steps 2 4 1 through 2 4 5 one time 1 Ifconcentric rings multiple patterns or any other distortions are still observed the fiber is damaged and a new fiber must be inspected Union Biometrica Inc Confidential 12 www unionbio com Final Set Up Adjustment Procedures According to UBI Operational Instruction OI 109 Final Set Up Adjustment Procedures Instructions Revision No 3 Last revision date 11 09 01 Purpose The purpose of this Ol is to list and describe the steps required to prepare a system for Final Test Before each COPAS BIOSORT instrument is delivered to a customer the system is tested according to the following procedures and should never need to be performed on site by a customer Some references to internal Union Biometrica documents are made for a copy of the referenced document p
44. ON e The system generates approximately 3800 BTU Hr e This instrument is rated IEC 60529 for installation in an IP00 environment It is intended that it be installed in a laboratory environment protected from dust and spray Air Requirements The input air pressure should be at least 50 psi but no more than 100 psi of filtered at 2 CFM of non condensing water and oil free air The optional COPAS Air Compressor is available for laboratories that cannot meet these requirements Union Biometrica Inc Confidential 9 www unionbio com Electrical Requirements e Place the COPAS system and components within 6 feet 2 meters to the power outlets e COPAS systems and the Laser should each be connected to separate dedicated electrical supply circuits using the supplied detachable line cords or CE7 VII approved equivalent detachable cords for Europe The supply circuits must be compliant with local electrical codes and must include a dedicated protective ground connection to protect the system The main supply voltage is not to exceed 1096 of the nominal voltage e Place the instrument so that the detachable line cords remain readily accessible This is essential to permit use of the line cords as the emergency disconnection devices in case of a fault Circuit Requirements for 120 VAC Countries o COPAS Instrument PC amp monitor 100 120VAC 15 Amp 50 60 HZ single phase on a dedicated line with protective earth ground
45. PER LEVEL E am voute c HV3C M 2 wo wm 2 ss 501 E R85 2 0K 502 18 1 12V _ a 17 082 7 504 16 083 10 RIS 4998 ig 084 vss gt an 506 4 085 12 086 GND S 507 0 0mv 499K 5 2551209 lt GND 47mV PER LEVEL MX7228KN n 1 072 als 499K spo 2 VALVE 3 501 4125 n 19 VALVEZ 1 0 0 502 5 xA 18 VALVES 2 299 503 VALVE4 cuo z LF412 11 7mV per level SD4 7 DA 16 VALVES H or 4 3 V of HV per level 505 8100 ce VALVES 2 33 gt 506 9 14 VALVE 557 10 13 VALVES 10 07 7 4249 12 1 GND EEY BRESET 13 STROBE 14 VALVLD 2j 57 e is et 4 E 16 5 7 UCN5801A GND i 20 R80 u79 5V 3i 499K spo sae om 20 VALVE9 7 zd RS103A u74 2 507 lon VALVETO E 1 2 i i lt 502 5 18 VALVETT STI 3 8 503 oUm cy VALVEI2 STR 25 100K 1 Fee E 2 504 7 eA 16 VALVEIS 6 85103 505 8 Ea 15 VALVE14 2 155 6 5 506 5 e pus 14 VALVEIS 2 id 2 507 10 13 VALVET6 07 OUT Sch 30 LM331 sie 100K O 24 31 4249 t gt us 1 R97 901 22 C80 GND OE 34 w 5 BLAST 20 0K GND UCN5801A 10 BLAST 7 33 36 V sac GND i
46. Tubing a 2 GP Sheath Reagent and MSDS 1 Cleaning Reagent and MSDS 1 Installation Disc a 1 Laser System Cable a 1 GP Control Particles and MSDS a 1 Bubble Trap Filter only with ReFLx a 1 Reference Manual 1 Information Packet Packaged by Date Inspected by Union Biometrica Inc Confidential www unionbio com Part Number 066 0004 000 066 0007 500 066 0009 010 066 0009 020 067 0020 004 067 0030 001 100 0010 001 111 0250 002 119 0003 000 145 0020 000 300 5044 000 300 5062 000 300 5068 000 300 5070 000 300 5072 000 300 5080 310 5018 000 310 5071 000 340 5014 000 350 5074 000 Date For Customers located Europe except United Kingdom Part Number Quantity 1 N RR hL Packaged by 350 5000 000 COPAS BIOSORT Description Power Strip Jumper Cables Power Strip Wall Cable Laser Wall Cable Compressor Wall Cable RS 232 Data Cable CAM Cable ReFLX ONLY Tubing Clamp PVC Air Tubing Threaded Barbed Air Fitting Slotted Screwdriver Waste Bottle Vent Tubing Waste Pump Tubing GP Sheath Reagent and MSDS Cleaning Reagent and MSDS Installation Disc Laser System Cable GP Control Particles and MSDS Bubble Trap Filter only with ReFLx Reference Manual Information Packet Union Biometrica Inc Confidential www unionbio com Serial Number Date
47. a LF444CM s gt R37 12 14 13 10K 732 R38 100K 4 1 c12 91 15v HV3_CTL Union Biometrica Inc 35 Medford Street Somerville 02143 Title PMT Preamp HV 3 Document Number lev E 350 5009 SCO A1 Date Wednesday April 30 2003 heet 4 of 7 COPAS BIOSORT PAGE 4 n cs 10pf Raa 100K 4 s JUMPER us NA m E R45 1K 21 6 8 DIN R47 R46 1 5 499K DIN P 357K N Di 3 2 R52 og vw vocm EMEN 332K gt R49 _ 91 145711 10 DY9 9 10K R50 EN OW DP 4 9 DY8 es 499K 8 DY 7 016 6 DY5 s vi gt 17145758 5 0 DY8 x m 4 DY3 RI 499 02 R54 z gt 2 cia 5 1 332 01 3 100K gt 20 17145758 72 T R104 499 R55 e 332K i 2 2 JUMPER R56 332K 0 5 24 R57 332K yu DY4 Co 15V 8 Vout 22 22 22 2 R58 22 ut 2 cw 332K ex F GND DY3 7 E
48. agency recognition Variation from these values will result in either unreliable operation E or unsafe operation FS ES m zn The silkscreen text is in error er E ES EI ECO Not An ECO of May 01 effectively disabled the SET 1 2 AA os oret 2 on vea drive signal by removing the driven end and shorting was al it to ground at the J9 connector on the Bulkhead To ssa ipa Panel Conflict with sheath pump motor control AA STANDBY RUNI AC MAIN ET AMBER inion Biometrica Inc E 3 our RSIC 5 Medford Street Suite 101 w 5 6 CUR PWRE SELT merville MA 02143 VPP10 3000 ATE en E AMBER TECRED EY Laser Interface uos cm m AA Document Number 310 5010 SCO B m AMBER Tuesday Apr129 2005 1 91 D 7 5 D 3 z T COPAS BIOSORT LASER INTERFACE
49. as 10 NU 19 VALVETG DINPUTI _ 20 Aud lt lt DINPUT2 n 2 lt lt Ax lt lt 12 2 A lt INTERLOCK 19 5 STI 13 25 STIRZ s 26 Existing Rework Not Showi 27 V16 becomes Optest old XCTL2 which HV2_CTL 2 Rs3 47 is pulled up to 15V 1 5 si 3 24V 32 T 34 lt 22 BLAST 37 28 gt LASERSET 19 INTERLOCK i VALVE2 7 a 5 VALVES E e 1 8 2 VALVE4 DIR 2 CON40 p 7 10 P VALVE 3 1 V 3 lt 6 VALVEI1 VALVE GND 4 5 11 5 lt gt VALVE12 TOLASER m 12 TOVALVE 2 ASSEMBLY i 5 ASSEMBLY 5 JUMPERBLOCK 8 5 3 TT 5V 6 PRESS1 ww Q9 E GND LASERMON lt lt PRESS2 E C D A LASERSET T 7 o PRESS 15 PRESS4 T 8 9 we 45V 7 Lo RI R2 R3 R4 0815 V pels 332 130K 221K 130K C 4 10 ain GND V GND 33 r ssp Di D2 D3 D4 LED LED LED LED ns 151 4249 gt gt 1 O 45V 2 9 4 ES END 47K Voc A 315 AUX POWER V V V 5 2 T 2 GND GND GND 157 P A Lo 7 4 1 8 5 46 GND DB9 swi V n2 1 12 E 1 GND 2s mE mE
50. laser to STAND BY mode b Close laser shutter c Remove the fiber from the instrument d Position the fiber approximately 12 30cm from a diffuse white target e Open the laser shutter f Examine the beam pattern for a uniform circular beam Union Biometrica Inc Confidential 11 www unionbio com g Ifa uniform circular beam is observed proceed to Installation Step 2 2 If concentric rings multiple patterns or any other distortions are observed go to Cleaning Procedure Step 2 4 and then repeat Steps 2 1 1 through 2 1 6 one time h Ifconcentric rings multiple patterns or any other distortions are still observed the fiber 15 damaged and a new fiber must be inspected 2 Installation Set the laser to STAND BY mode Close laser shutter Soak a cotton swab with 100 Methanol Clean the fiber receptacle with the soaked cotton swab Insert the fiber into the receptacle in the optical assembly ensuring the fiber face does not come in contact with the flat surface in between the threaded ring and the receptacle bore 3 Installation Inspection a Open the laser shutter b Place beam blocking device into the beam path between the spherical lens and the cylindrical lens mount c Ifa uniform circular beam is observed installation is complete If concentric rings multiple patterns or any other distortions are observed go to Cleaning Procedure Step 2 4 and then repeat Steps 2 3 1 through 2 3 2 one time d
51. sample container while pressure is on Foaming will occur NOTE The secondary sample container may also be washed in a dishwasher or autoclaved if preferred Union Biometrica Inc Confidential 27 www unionbio com 6 Attach sample tubing to the primary sample cup 7 Re check the SAMPLE VALVE checkbox The warning message Caution this Operation will contaminate the Flow Cell if Sample is Present will appear Click OK 8 Process the Cleaning Reagent until the sample cup is empty 9 Click OFF the SAMPLE VALVE checkbox 10 Detach the sample tubing and remove the cap from primary sample cup 11 Fill the primary sample cup with 40 mL of deionized distilled water 12 Aspirate deionized distilled water and discard 13 Fill primary sample cup again with 40 mL of deionized distilled water and firmly tighten the cap 14 Attach sample tubing to the primary sample cup 15 Select Acquire and process the deionized distilled water for approximately 10 minutes Do not allow the sample cup to run dry 16 Click the STOP button twice 17 Clean external surfaces of any spillage using a water dampened lint free cloth NOTE Do not remove or clean any of the electrical connectors or cables of the COPAS BIOSORT Visual Tubing Check Depending on the frequency of instrument use it is recommended that a visual inspection of the system and tubing be made If the instrument is used daily it is recommended that the external tubing be in
52. 0 5020 000 350 5027 000 350 5041 000 350 5039 000 350 5021 000 350 5023 000 300 5018 000 340 5000 000 340 5001 000 Description COPAS BIOSORT Air Regulator Filter Assembly Valve Panel Assembly Sample Bottle Assembly Waste Tray Assembly Waste Pump Assembly Back Plate Base Plate Final Optics Assembly System Electronics Assembly Sample Bottle Support Assembly Sample Cup Assembly Argon Laser System Assembly Laser Cover Assembly XY Stage Assembly ReFlex Sampler Assembly Optional ReFlex Sampler Valve Panel Assembly Optional Drawings matching these part numbers descriptions immediately following this page Union Biometrica Inc Confidential 32 www unionbio com 2 lt 36 iud lt gt 210 Z gt 2 Ola gt lt al gt 2 Ola oa MIO alee lt gt lt gt x e 2 Oa 2 gt gt lt ue gt ez Qoo lt 5 4 lt lt gt mug au e 5 gt 2 2 5 lt 4 5 lt lt gt Peo ture H 6 max s gt E usu z gt gt 5 55 75 lt lt gt 5 2 5 4 3 gt lt o uq uq aq Suq lt 2 0 0
53. 0 5072 000 is shipped with each COPAS BIOSORT instrument Bleach Bleach is used on the COPAS instrument for troubleshooting procedures including breaking up proteins potentially causing a blockage in the flow cell or fluidics Bleach is also used to clean the internal flow cell The manufacturer does not supply ethanol NOTE DO NOT use on external surfaces of optical components UNION BIOMETRICA INC recommends that Clorox brand bleach be used due to its observed low particulate count Ifa different brand of bleach is used filtering is required When using bleach use 5 sodium calcium hypochlorite at a 50 dilution Sterilization Solution 70 Ethanol is used on the COPAS instrument for sterilization procedures and in certain troubleshooting procedures The manufacturer does not supply ethanol Union Biometrica Inc Confidential 8 www unionbio com Materials Required for Service Not Provided Screwdrivers 10 length and Jewelers size Oscilloscope amp wires Needle nose pliers Paper towels Cable ties to secure tubing and cables 50 mL syringes for removing liquid from the sample cup 96 well plates and lids e Argon Laser Alignment Glasses 2 3 488 515nm amber lens 70 VLT Only required if service will be performed on the Argon Laser by the customer Union Biometrica highly recommends that any laser service be performed by a Union Biometrica trained service person Work Area and Facilities Specifications
54. 074 000 Date Reagents Used Do not use unapproved solutions in the COPAS BIOSORT The use of unapproved solutions can cause damage to the device and will void the warranty The specialized reagents discussed below are available from Union Biometrica Inc Material Safety Data Sheets are enclosed in every shipment of Union Biometrica Inc supplied reagents and are also available upon request NOTE Performance Specifications are only valid with Union Biometrica Inc reagents Sheath Reagent Sheath reagent is an aqueous based reagent containing surfactant The sheath reagent must be compatible with the control particles used and the sample to be analyzed For most applications COPAS GP Sheath PN 300 5070 000 is recommended Two 20 Liter containers of PN 300 5070 000 are shipped with each COPAS BIOSORT instrument Control Particles Control particles are latex beads that are uniform in size suspended in a reagent compatible with the sheath reagent and sample to be analyzed For most applications GP 42 micron HF Control Particles PN 310 5071 000 are recommended One Liter container of PN 310 5071 000 is shipped with each COPAS BIOSORT instrument Cleaning Reagent Cleaning reagent is an aqueous based reagent containing surfactant specially formulated for use with all COPAS platforms This solution should be used for daily cleaning procedures Cleaning reagent should be used for daily maintenance One Liter container of PN 30
55. 10 Open TOOLS Menu and select SELECT PMT FILTERS Change to Filter Set 1 11 Adjust Green PMT on the computer main screen to obtain a voltage output of 1 5 volts on the oscilloscope Union Biometrica Inc Confidential 18 www unionbio com COPAS Instrument Test According to UBI Procedure 350 5000 TPR COPAS BIOSORT Test Procedure Revision No 2 Last revision date 9 26 01 Instrument Set Up 1 Ensure air supply is connected to the instrument and turned ON Set pressure to 20 25 psi 2 Turn ON instrument main power and laser power supply 3 Turn ON computer and record the following E Prom Number Instrument Serial Number Registration Number Software Rev Firmware Rev 4 Measure laser power Start the instrument and document the laser power from the main screen and the laser power supply Specification Blue Laser Power mW 10 1 0 mW Main Screen Laser Power mW t 1 0 mW from Blue Laser Power Supply Laser Power mW t 1 0 mW from Blue Laser Red Laser Power mW 0 8 1 05 mW 5 Setup pressures Place approximately 4 L of BIOSORT Sheath Reagent P N 350 5071 000 into the sheath container and cap firmly a Place approximately 4 L of BIOSORT Sheath Reagent P N 350 5071 000 into the sheath container and cap firmly b Place approximately 200mL of distilled water into the secondary sample container and cap firmly c Place approximately 500mL of BIOSORT Sheath Reagent P N 350 5071 000 into the cleanout bottle d Sele
56. 2_CTL 21 2 FL2 Oa ao 0a 24 44 12 2 FL2 gt 20 2 7 d 11 OT XCTLSEL 1 EXT 10 2 PMT HV2 Preamp2 V High Voltage 4 High Voltage 5 EN up Cm FL3 gt 4 4 FL3 15 10 D6 94 gu PMT P gt l agp Preamp3 14 PMT_HV3 of C ak 1 ey Z R102 A XCTL2 TOEXTINCTION 12 e 7 732 DETECTOR 04 7 24 e 11 XCTLSEL ots EXT 1 EXT 6 PMTTest 2 HDB15 NA x XCTL2 xc 522 Union Biometrica Inc 35 Medford Street Somerville 02143 Title PMT Preamp HV Top Level ize Document Number lev 5 350 5009 SCO Al Tuesday April 29 2003 heet 1 of 1 COPAS BIOSORT PMT PAGE 1 HVPS1 ADVANCE GM121K5NS HV1_CTL R9 1000 R15 3 NO a 1 2 Ay Vin Vout Vin Vout gt LM317EMP R2 22 lt 2 243 w E 15V_HV x 9 a m mn LF444CM 100M i 4 p a LF444CM UID 5 50 d gt LFAMCM 24 R7 10 R8 2N3904 10K R9 12 m 14 d 13 10K 732 R10 100K 10K 1 15V_HV 15V a 91 6 PM RIS
57. 300 5019 000 300 5048 000 300 5055 000 310 5018 000 335 5064 000 340 5006 000 340 5007 000 350 5010 000 350 5011 000 350 5012 000 350 5013 000 Description XY Internal C A Stage C A Valve Panel C A Laser Control Blaster C A Frontside C A Backside C A PMT C A Detector C A Stirrer C A Sheath C A Union Biometrica Inc Confidential www unionbio com Board Revision No RevA RevA RevA RevA RevA Rev 5 Rev 7 Rev 1 Rev 2 Rev 2 Rev 2 Parts List Revision No RevA RevA RevA RevA RevA Rev 5 Rev 5 Rev 1 Rev 2 Rev 2 Rev 2 Notes ReFlx option ReFlx option 33 D 7 5 s D 3 z T enno APP
58. 5 Adjust the sort parameters to the following Sort Delay Sort Width Per Well Co 6 Ensure 10 4 rate incidence Sort Parameters 8 1 0 5 7 1 7 Print screens of dot plots showing TOF vs EXT and EXT vs FLU Union Biometrica Inc Confidential www unionbio com 2 events worms sec If rate is above this target dilute sample to achieve this 22 8 Adjust markers EXT to gate approximately 60 of the worms Record actual value Gate Value Record the Sort Region Low igh of Events TOF EXT FLU1 FLU2 9 Place 96 well tray onto stage and Select LOAD PLATE 10 Select FILL PLATE and start timer Upon completion of plate filling record the time in seconds Specification Time S lt 105 Seconds 11 Print the display screen and attach to this document 12 View full tray with a microscope using a 4X or 10X objective Record results on Worksheet Specification of wells with lt 1 worm lt 2 of wells with gt 1 worm lt 3 13 Change Per Well to 5 and select ACQUIRE to begin particle flow 14 Place 96 well tray onto stage and Select LOAD PLATE 15 Select FILL PLATE and start timer Upon completion of plate filling record the time in seconds Specification Time S lt 180 Seconds 16 Print the display screen and attach to this document 17 View first 24 wells with a microscope using a 4X or 10X objective Record results on
59. 665 22 22 gt gt Union Biometrica Inc 35 Medford Street Somerville MA 02143 Title PMT Preamp HV HV1 ize Document Number lev 350 5009 5 0 1 Date Wednesday April 30 2003 sheet rs of 7 COPAS BIOSORT PMT PAGE 2 HVPS2 ADVANCE GMI21KSNS VR2 2 5 Vout 2 3 5 Oo vin Vout 2 Lu 15V_HV amp LM317EMP G 8 8 2 2 W 243 R23 HV2_CTL R23 1000 R29 V 15V HV U2A gt LF444CM u28 mr LF444CM 2 m RI9 gt 100M LF444CM wn is bd LFAMACM 2 7 R21 10 R22 2N3904 R23 12 m M 13 10K 732 R24 15V 100K a 827 10K 4 1 R29 15V_HV asv ma L 2 gt c ce 22 22 Union Biometrica Inc 35 Medford Street Somerville 02143 Title PMT Preamp HV HV2 ize Document Number lev E 350 5009 SCO A1 Date Wednesday April 30 2003 sheet 5 of 7 COPAS BIOSORT PAGE 3 HVPS3 ADVANCE GM121KSNS VR3 Vout 3 4 1 2 Vin Vout T 15V_HV gt LM317EMP R30 a a HV3_CTL R37 1000 R43 22 22 243 w gt NS 15 HV Gi gt LFAMACM U3B ud 100M 174K a0
60. ACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC WHICHEVER COMES FIRST REV REVISIONS amp DESCRIPTION DATE BY APPROVED INITIAL WORKING DRAWING 02 05 03 J M M UNLESS OTHERWISE SPECIFIED DIMENSIONS ARE IN INCHES DRAWN JMESSINA 02 05 03 TOLERANCE ON DECIMALS ANGLES ENGINEER i XX 005 30 APPROVED UNION BIOMETRICA INC SOMERVILLE MA 617 591 1211 CONCENTRICITY 005 TIR REMOVE BURRS amp SHARP EDGES PROJ QUALITY ALL FINISH SURFACES BE32 ASSUR TITLE MICROINCHES UNLESS NOTED ADDITIONAL COMMENTS 550 5000 000 APPLICATION WHERE USED MATERIAL NONE FINISH NONE ASSEMBLY LASER COVER SIZE PART NUMBER 350 5023 000 REV SCALE 1 2 DO NOT SCALE THIS DRAWING SHEET 1 OF 1 THIS DRAWING IS SOLE PROPERTY UNION BIOMETRICA INC AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER THE CONTENTS ARE FOR THE PURPOSES ONLY OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC AND SHALLNOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC WHICHEVER COMES FIRST REVISIONS amp DESCRI PTION
61. Area Requirements nn nee ee e e E ode 9 Environmental Requirements nenne e Fee 9 Air ete D i IR E rud 9 El ctrical Requirements rt t eeu a nee ede p 10 UNPACKING THE COPAS INSTRUMENT uo da datus 11 OPTICAL FIBER INSPECTION INSTALLATION AND CLEANING cerent 11 TEES 1 FINAL SET UP ADJUSTMENT PROCEDURES 13 Set UP ee a ee 13 Software Verification RE TR PER ERRARE 14 Fl idics Check ere 15 Laser Power Adjustment s a u d re 16 Waste Tray 17 PMT Set 18 COPAS INSTRUMENT BST 19 Instrument e II upa qaku has 19 Performance Set Up 20 Performance Verification 22 Sorter Operation VertficatiOn asnasan Ee Pe e EH EH ERE PES 24 Instrument Preparation ore nieder ES 25 Instrument Test Worksheets insonni uneni tie heri 26 DAILY SHORT TERM COPAS INSTRUMENT MAINTENANCE
62. ER PK AC SOMERVILLE 617 591 1211 005 30 TR aes PRON a QUALITY ASSEMBLY ALL FINISH SURFACES BE 32 ASSUR MICROINCHES UNLESS NOTED DDITIONAL COMMENTS VALVE PANEL 350 5000 000 MATERIAL NONE SIZE PART NUMBER REV APPLICATION FNS 300 5037 0000 1 WHERE USED SCALE 1 2 DO NOT SCALE THIS DRAWING SHEET 1 OF 1 THIS DRAWING SOLE PROPERTY UNION BIOMETRICA INC AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER THE CONTENTS ARE FOR THE PURPOSES ONLY OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC AND SHALLNOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC WHICHEVER COMES FIRST REV REVISIONS amp DESCRIPTION DATE MADE BY APPROVED INITIAL WORKING DRAWING 07 18 02 J M M UNLESS OTHERWISE SPECIFIED DRAWN J MESSINA 07 18 02 DIMENSIONS ARE IN INCHES UNION BIOMETRICA INC TOLERANCE ON ENGINEER KASSEY 07 18 02 SOMERVILLE MA 617 547 7703 DECIMALS FRAC ANGLES E 1010 30 APPROVED 005 PROJECT QUALITY ASS EM B LY CONCENTRICITY 005 TIR ENGR ASSUR REMOVE BURRS amp SHARP EDGES SAMPLE BOTTLE ALL FINISH SURFACES 2 APPI
63. EV 300 5061 000 B SCALE 1 1 DO NOT SCALE THIS DRAWING SHEET 1 OF 1 THIS DRAWING IS SOLE PROPERTY UNION BIOMETRICA INC AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER THE CONTENTS ARE FOR THE PURPOSES ONLY OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC AND SHALLNOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC WHICHEVER COMES FIRST ITEM NO QTY PART NO DESCRIPTION 070 0020 002 MOTOR STEPPER 131 0440 009 SCREW 4 40 X 7 16 131 0832 001 SCREW PH 8 32 X 1 2 139 0003 001 MOUNT ISOLATION 300 1061 000 ROLLAR UNLESS OTHERWISE SPECIFIED DIMENSIONS ARE IN INCHES TOLERANCE ON DECIMALS FRAC ANGLES 010 005 30 REV REVISIONS amp DESCRIPTION DATE MADE BY APPROVED 1 INITIAL WORKING DRAWING DRAWN J MESSINA 06 11 02 ENGINEER KASSEY 06 11 002 06 11 02 UNION BIOMETRICA INC SOMERVILLE MA 617 547 7703 APPROVED 300 1062 000 MOUNT PUMP MOTOR PLATE PUMP SUPPORT 300 1064 000 ROLLER PUMP CONCENTRICITY 005 TIR REMOVE BURRS amp SHARP EDGES ALL FINISH SURFACES TO BE 32 MICRO INCHES UNLESS NOTED PROJECT z ENGR A NAME QUALITY ASSUR A NAME
64. FORM WHATSOEVER THE CONTENTS ARE FOR THE PURPOSES ONLY OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC AND SHALLNOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC WHICHEVER COMES FIRST REV REVISIONS amp DESCRIPTION DATE MADE BY APPROVED INITIAL WORKING DRAWING 04 22 02 J M M UNLESS OTHERWISE SPECIFIED DRAWN J MESSINA 04 22 02 DIMENSIONS ARE IN INCHES UNION BIOMETRICA INC TOLERANCE ON ENGINEER KASSEY 04 22 02 SOMERVILLE MA 617 591 1211 DECIMALS FRAC ANGLES TIE XX EOI APPROVED XXX 005 PROJECT QUALITY ASSEMBLY SOUND OR ML NE ALL FINISH SURFACES APPITIONAL COMMENTS ARGON LASER SYSTEM MICRO INCHES UNLESS NOTED x MATERIAL NONE SIZE PART NUMBER REV ALL APPLICATION FINISH C 350 502 1 000 WHERE USED NONE SCALE 1 3 DO NOT SCALE THIS DRAWING SHEET OF 1 THIS DRAWING IS SOLE PROPERTY UNION BIOMETRICA INC AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER THE CONTENTS ARE FOR THE PURPOSES OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC AND SHALL NOT BE USED ASA BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUF
65. LESS NOTED MATERIAL NONE SIZE PART NUMBER REV TOP LEVEL C hee C APPLIC ATION FINISH NONE WHERE USED SCALE NONE DO NOT SCALE THIS DRAWING SHEET 1 OF 1 69 20 23 QTY PART NO 29 9 11300 1013 000 18 1 340 1006 000 19 11340 1001 000 20 11340 1002 000 21 11340 1004 000 22 1 340 1007 000 23 11340 1003 000 24 1 340 1012 000 26 _ 1 300 5011 000 NLESS OTHERWISE SPEC IFIED UNION BIOMETRICA INC 27 21340 1010 000 ASSES SOMERVILLE MA 617 547 7703 28 1 340 1005 000 29 11070 0040 002 5 30 11199 0006 002 22 SAMPLER ASSEMBLY 31 1 199 0006 001 32 1 120 0003 003 1 340 1008 000 50000 00 Printed Circuit Boards Assembly 310 5010 000 340 5005 000 350 5001 000 350 5002 000 350 5008 000 350 5009 000 Description Laser Interface ReFlx Sampler Sorter Controller Bulkhead Connector Extinction Detector PMT Preamp HV Board Revision No Rev B Rev B Rev D Rev A Rev Rev A Parts List Revision No Rev C2 Rev 4 Rev DI Rev A3 Rev A3 Rev Notes ReFlx option Drawings matching these part numbers descriptions immediately following this page Major Cable Assemblies Assembly
66. P6 JUMPER k VR8 15V 8 Vout 1 V3 2 3 GND GND 6 H m 7 GND 78L12 sm VR9 2 1 12 Vout 3 6 7 5 GND 79L12 sm Union Biometrica Inc 35 Medford Street Somerville MA 02143 Title PMT Preamp HV Flu3 PMT ize Document Number lev 350 5009 5 0 1 Date Tuesday April 29 2003 heet 2 of 7 COPAS BIOSORT PAGE 7 Contact Information Union Biometrica Inc has offices in the United States and Europe US Global Headquarters Union Biometrica Inc 35 Medford Street Suite 101 Somerville Massachusetts 02143 Tel 617 591 1211 Fax 617 591 8388 Email sales unionbio com Europe Union Biometrica N V European Scientific Operations Cipalstraat 3 B 2440 Geel BELGIUM Tel 32 0 14 570 620 Fax 32 0 14 570 621 Email sales unionbio com COPAS is a registered trade mark of Union Biometrica Inc Twister is a registered trade mark of Zymark Corporation Union Biometrica Inc Confidential www unionbio com UNION BIOMETRICA A Harvard Bioscience Company 34 Notes Union Biometrica Inc Confidential www unionbio com 35 Union Biometrica Inc Confidential www unionbio com 36
67. R REV 350 504 1 Q00 1 SCALE NONE DO NOT SCALE THIS DRAWING SHEET 1 OF 1 THIS DRAWING IS SOLE PROPERTY UNION BIOMETRICA INC AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER THE CONTENTS ARE FOR THE PURPOSES OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC AND SHALL NOT BE USED ASA BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC WHICHEVER COMES FIRST REV REVISIONS amp DESCRIPTION DATE BY APPROVED INITIAL WORKING DRAWING 07 15 02 J M M UNLESS OTHERWISE SPECIFIED DRAWN J MESSINA 07 15 02 DIMENSIONS ARE IN INCHES DECIMALS FRAC ANGLES ENGINEER KASSEY 07 15 02 10 4 XX XXX 005 S APPROVED CONCENTRICITY 005 TIR REMOVE BURRS amp SHARP EDGES PROJ QUALITY ALL FINISH SURFACES 32 ENG ASSUR UNION BIOMETRICA INC SOMERVILLE MA 617 591 1211 MICROINCHES UNLESS NOTED ADDITIONAL COMMENTS 550 5000 000 APPLICATION WHERE USED MATERIAL NONE FINISH NONE ud ASSEMBLY SAMPLE CUP SIZE PART NUMBER 350 5039 000 REV SCALE 1 2 DO NOT SCALE THIS DRAWING SHEET 1 OF 1 THIS DRAWING SOLE PROPERTY UNION BIOMETRICA INC AND SHALL NOT BE REPRODUCED IN ANY
68. ROVAL DATE STANDARD JUMPER CONFIGURATION owa JP Not installed NAE 1 External Interlock Switch Active NOT installed JP2 Installed 2 2 Ignore Interlock INSTALLED s TO INTERLOCK 1 oci JP3 Force Local Mode INSTALLED 2 2 SWITCH ZN wa Leg coa se ee 511 5 E on 8 7 HCPL2211 b REED RELAY INTUK INA 2 1 Tle 2 5 Force Local Mode np 218 gps ya viven Punicum Installed D 5 TO J9 ON BULKHEAD PANEL s w c varven STANDBY RUN2 3 E HCPL 2211 Ku Via 310 5018 000 C A 1 1 ao o e 9 vieta os ortos 7159 E r 1 3 B ep d LASERMON 09 o1 adi 5 7 2 SUNSTANDEYI w 3 n 519 TOLASER Te I 1 T lt H ST BTANDBY RUNIE MSOF Deis oF 1 POWERSUPPLY PSI CURRENT MONITOR d os Tle us 2 s zi Via 310 5017 000 Cable F 4 3 TIN V T PWRCTL La or GND2 z HCPL2211 15 bs oris Te6 o g I ons MONITOR E E E GAIN ocs me E Radded 499 4 5 558 A99Kadded 6 7 01 3 5 Go jpo to desensitize 0 12 s adjustment range er 5 200K He if 245 31
69. TIONAL COMMENTS MICRO INCHES UNLESS NOTED x MATERIAL NONE SIZE PART NUMBER REV 350 5000 0000 APPLICATION FINISH C 300 5059 000 WHERE USED NONE SCALE 1 1 DO NOT SCALE THIS DRAWING SHEET 1 OF 1 THIS DRAWING IS SOLE PROPERTY UNION BIOMETRICA INC AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER THE CONTENTS ARE FOR THE PURPOSES OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC AND SHALL NOT BE USED ASA BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC WHICHEVER COMES FIRST REV REVISIONS amp DESCRIPTION DATE BY APPROVED B INITIAL WORKING DRAWING 07 19 02 J M M DIMENSIONS ARE IN INCHES UNLESS OTHERWISE SPECIFIED DRAWN J MESSINA 07 19 02 TOLERANCE ON DECIMALS FRAC ANGLES ENGINEER KASSEY 07 19 02 010 4 ed XXX 005 APPROVED UNION BIOMETRICA INC SOMERVILLE MA 617 547 7703 CONCENTRICITY 005 TIR 370 5000 000 REMOVE BURRS amp SHARP EDGES PROJ QUALITY ALL FINISH SURFACES BE 32 _ ENG ASSUR 350 5000 000 MICROINCHES UNLESS NOTED 335 5000 000 MATERIAE APPLICATION WHERE USED NONE ADDITIONAL COMMENTS WASTE TRAY ASSEMBLY SIZE PART NUMBER R
70. TOR2 gt 52 5 19 0135 xen 12 1 S2_wB2 2 i 20 2 lt ladj 5 AU xci 2 s r gt 2 xm Pon LASERMON ales LL xe 1 5 e e gt lt 1 500 25 7 1 AUXI 15V EXT 10 IB462H i 26 TP6 a la n7 c6 M CON26 V 33 53 5 57 AUX2 S S3 WA2 5 GND HD26 lt 10 gt S3 WB1 LEES 3 W82 AUG 1 NZ ov c lt P DINPUT3 yer as MOTOR3 woe 220 2 gt PRESS1 RS2 2 1 u2 DINPUT4 6 10 52 DIR 1 12 BS3_PLS 4 zy J 5 S2 HFS 2 n 53 WAT RE PRESS2 E 8 ZR 3 10 DINPUT2 53 DIR LE pt m 12 DB9 7 53 DIR 4 J ten E 6 53_ 5 5 8 S3_HFS aw eae Lo 53 WA2 PRESS3 V sei ce SAP 5 6 7 DINPUT3 m 19 4 BS3_AWOFF T GND 3 JUMPER BLOCK 12 n6 PRESS4 3 5V m DR 53 WBl 7 I O 20 1 x 20K Wk xm 14 mA 0 53 WB2 215 t M E pa 518 PXHILIM 15 S3_R 2 3 4 610 UPEA 16 C GND lt 1 2 amp 5 500 17 x 4 5 Existing Rework Not Shown s IB462H ilo ab ep a a 5 1 c oe I O 2 V16 becomes Optest old XCTL2 which cn ca O ae 5 RXD GND lt 2 1 Te7 is pulled up to 15 via 1 5K 10 33 715 STAGE
71. V E o NX D3 5 V NAA 2 XCTL2 wv i 7 1 gt LED 0 n 12 R12 R13 CON12 499K 499K m SHIELD co as 8 25 618 UN Vout c c gt 22 22 22 22 2 GND 4 4 4 eu 2 GND JP1 JP2 amp JP3 are installed 3s t GND V GND U4 amp U5 not installed at v SZ 78L12 iei 7 m current revision 22 22 2 m 22 31 Vm Vout vin 7 inion Biometrica Inc 5 aD BY 22 5 Medford Street Suite 101 79L12 sm omerville 02143 V Title HECKED EY Extinction Detector amp Preamplfier ize Document Number pate B 350 5008 SCO A3 Date Tuesday April 29 2003 heet 1 of 8 6 5 3 2 1 COPAS BIOSORT EXTINCTION DETECTOR High Voltage 1 High Voltage 0 49 5 SS FU 9 FL3 e Pam FB 26 FL2 9 8 FL2 17 25 16 15 PMT_Preamp1 6 _ 15 1 gt 23 o gt 2415V HV High Voltage 2 High Voltage 3 TO BULKHEAD yale Or CONNECTOR 4 Hv lt 3 HV
72. Worksheet Refer to Section 7 13 Sort Parameters in the Operator s Manual to learn more and if results are not within specifications Specification of wells with lt 5 worms lt 2 of wells with gt 5 worms lt 3 Testing Performed By Date Performed Union Biometrica Inc Confidential 23 www unionbio com Sorter Operation Verification 1 2 Turn Sheath and Sample Valves ON Set SORTER Pressure to 3 0 Place a microscope slide under the sort hole on the waste tray and run for a minimum of minute Inspect the microscope slide for spray Spray Visible Yes No If No proceed to step 6 If yes follow the below troubleshooting steps h Check sheath and sample containers Verify they are filled appropriately Check sheath and sample pressures Verify they have reached the specified levels Check the waste container Verify it is draining properly Check the waste tray Verify it is pushed all the way to the right Check the outlet nozzle Verify that liquid has not accumulated around the nozzle If it has perform the following e Remove the waste tray e Soak up excess liquid around the nozzle with the tip of a paper tissue e Return the waste tray to position observe if spray is still present Check the flow cell for a clog Clear the flow cell by performing the following Click the CLEAN button several times e Place a finger over the vent on the sheath bottle cap and then rem
73. aintenance worksheets are found in the APPENDIX section of the Operator s Manual e Daily Performance Log Sheet e Maintenance Log Sheets Daily Short Long Term Shutdown Sterilization e Troubleshooting Log Sheet Union Biometrica recommends that these be used to store important instrument information Recording performance data regularly can aid in the identification of subtle shifts in readings which may indicate system problems Please make copies of these maintenance worksheet pages as needed Daily Maintenance Procedure NOTE Do NOT leave sample or Cleaning Reagent in the instrument over night 1 Click OFF the SAMPLE VALVE checkbox 2 Detach the sample tubing and remove cap from primary sample cup 3 Discard any remaining sample 4 Fill primary sample cup with approximately 25 mL of Cleaning Reagent Replace the cap and firmly tighten 5 Clean the secondary sample container if used as follows a Add 200 mL of Cleaning Reagent to the secondary sample container b Detach the aerators and re cap the container c Shake the container to clean the interior surfaces d Empty the Cleaning Reagent from container and rinse the container thoroughly with deionized distilled water Visually inspect the container for any remaining sample residue Add 200 mL of deionized distilled water to the container f Clean and reattach the aerators Replace the cap and firmly tighten e NOTE Do not put Cleaning Reagent into the secondary
74. based on the size of the flow cell 250 500 1000 Sheath 4 0 5 0 Sheath 2 5 3 5 Sheath 4 0 5 0 Sample 5 0 7 0 Sample 1 2 1 8 Sample 2 0 3 0 Sorter 3 0 4 0 Sorter 3 0 4 0 Sorter 3 0 4 0 Cleaning 10 0 11 0 Cleaning 10 0 11 0 Cleaning 10 0 11 0 5 Select Pressure OK 6 Select Sample Valve and run until cup is empty 7 Rinse Sample Cup with distilled water three times 8 Fillsample Cup with distilled water and run until cup is empty 9 Refill Sample Cup with distilled water 10 Turn Sheath Valve ON and check for flow 11 Turn Sample Valve ON and check that the valve opens and closes Ensure that there are no leaks 12 Turn Sample Valve OFF 13 Select Clean and verify flow increases through sheath tubing 14 Turn Sheath Valve OFF 15 Verify mixers are spinning 16 Turn Sheath Valve ON and Measure the flow rate Adjust the sheath pressure to obtain a flow rate as follows 250 500 1000 9 10 ml min 20 25 ml min 30 35 ml min Union Biometrica Inc Confidential 15 www unionbio com Laser Power Adjustment 1 Using a Laser Power Meter set to 635nm place the power paddle between the laser and the cylindrical lens until the maximum meter reading is located 2 Adjust the screw on the POT located on the laser supply board to obtain EXT voltages as follows 250 500u 1000 2 5 3 0 volts 1 0 1 5 volts 2 5 3 0 volts within an approximate las
75. ct START on the main screen and wait approximately 1 minute for pressures to equilibrate e Verify both stir bars are spinning If not turn mixer OFF re position stir bars and turn mixer back ON If stir bars are still not mixing troubleshoot instrument f Make sure that all lines are air free g Turn SORTER Pressure OFF Measure the flow rate of the Sheath The flow rate of the sheath must be 8 10 ml min Adjust pressure to obtain this flow Flow Rate Final Pressure h Adjust sample pressure to obtain minimal counts sec Adjust the following 1f necessary and record final pressures Union Biometrica Inc Confidential 19 www unionbio com NOTE Sample pressure must be at least 1 0 psi greater than sheath pressure Settings Actual Sheath 3 5 5 0 psi Sample 5 0 7 0 psi Sorter 3 0 4 0 psi Clean 9 0 11 0 psi Performance Set Up 1 Select PRESSURE OK 2 Open the FILE Menu and select the 96serp file 3 Clean the Sample Cup Sample Valve and Flow Cell According to UBI Procedure OI 05 Sample Cup Sample Valve and Flow Cell Cleaning Revision No A Last revision date 9 29 00 a b Bu 6 5 mop q Click OFF the SAMPLE VALVE checkbox Detach the sample tubing and remove cap from primary sample cup Discard any remaining sample Fill primary sample cup with 40 mL of deionized distilled water and firmly tighten the cap Reattach sample tubing to the primary sample cup Re c
76. d bi e 5 GND CON40 24V V GND gt ww 4 ED EE 2 5 6 D BLAST IN 4 R75 6 3 7 d min inion Biometrica Inc o 15K P 5 Medford Street Suite 101 T iomerville 02143 R76 UDN2962W R78 m cis cs p 02 Sii 10 33 33 33 33 Title 619K j e 674 Sorter Controller Valve amp Motor Drivers GND 38 33 ize Document Number B 350 5001 SC0 D1 5 GND GND GND GND Date Tuesday April 29 2003 heet 3 of 10 8 7 6 5 4 3 2 1 COPAS BIOSORT PAGE 3 8 7 6 4 3 2 1 RS3 5V 2 8500 2 BSD1 3 BSD2 4 8504 6 gt 8505 7 FL3 BSD6 8 8507 5 5 lt 0 7 D 6 47K 2 LF412 gt n u2 x is gt EXTSIG 1 REF p 16 20K 85 100K 880 vo UB 8501 1 15 D4
77. e free of defects at the time of installation Union Biometrica does not warrant parts and accessories not manufactured by or for Union Biometrica Union Biometrica will assist buyer in obtaining any information or service from any manufacturer for any part not covered by Union Biometrica 2 Freight charges are at the customers expense The warranty is invalid if 1 The COPAS platform is used for purposes outside the scope of supported applications and specifications 2 System modifications not approved by Union Biometrica in writing are performed Warnings and Precautions The COPAS BIOSORT is a Class I laser product containing a Class laser The COPAS Argon Laser System contains a Class III B laser which is fiber coupled through a fiber optic Only qualified service personnel should remove the COPAS Argon Laser System or the optics assembly covers The COPAS BIOSORT is comprised of the Dispenser and the Argon Laser System This term is used in reference to the combination of these two components Use of the COPAS BIOSORT Dispenser and Argon Laser System in a manner not specified by Union Biometrica may impair the protection provided by the equipment The COPAS instrument will be factory shipped to you in protective packaging and will be installed by a trained Union Biometrica Inc representative The Union Biometrica service representative will also verify the system performance If the instrument must be moved or maintenance
78. e Int Adj located in the bottom of the Argon laser g Set the Argon laser readout to within 1mW of the laser power recorded in step 3 by adjusting POT 3 DVM ADJ h Set main screen laser power to within ImW of the laser power recorded in step 3 by adjusting POT 1 MON GAIN On the main computer screen open TOOLS Menu and select Run External Laser Change laser to the Standby Mode With the laser power meter still in place record the laser power Compare the Argon laser readout and the main screen laser power to the laser power meter Adjust the three readings to be as close as possible Set the laser back into Run Mode and verify the three readings in Run Mode still meet the ImW specification If not readjust to be within specification and repeat steps 3 1 through 3 M B Software Verification 1 With computer powered up verify the following a WINMDI software present b Powerware software present Registration Number corresponds with System Type and Serial Number d INI file values set according to attached samples Union Biometrica Inc Confidential 14 www unionbio com Fluidics Check 1 Measure the length of the waste pump tubing 2 Connect and turn ON External Waste Pump if applicable 3 Add approximately 20ml of filtered bleach to Sample Cup 4 Select START and allow the system to pressurize Adjust the pressures to the following
79. er power range of 250 500u 1000 0 75 1 25 mW 1 5 2 5 mW 0 5 1 0 mW Optical Fine Tuning NOTE Use proper laser safety precautions at all times while performing these steps 1 2 8 9 Connect channel 1 on the oscilloscope to the EXT Test Point on the Pre Amplifier Connect channel 2 to the Green PMT Set up the oscilloscope using a 1X probe with settings of 1 00 v on both channels and 25 0 ms Fill Sample Cup with appropriate beads and Turn Sheath and Sample Valves ON Turn the flow cell adjuster screw on the optical assembly to obtain maximum peak height on the oscilloscope Move the Cylinder Lens toward the flow cell to obtain the narrowest 25 5 wide with rounded bell shaped curve Do not allow the top of the peak to flatten Remove the EXT detector and place the mask onto the optical assembly Align the sensor mask by centering the red line on the laser beam Place the rod holder spacer and EXT detector onto the optical assembly 10 Print a copy of the ini file and place into the DHR Diverter Alignment 1 Position the Sorter Valve 1 stream diameter away from the flow stream 2 Turn sheath valve and sorter valve ON 3 Looking directly at the spray of sheath adjust both the front and back diverter adjustment screws until the spray is centered 4 Adjust the Sorter pressure to obtain a stream deflection of 30 from vertical Union Biometrica Inc Confidential
80. heck the SAMPLE VALVE checkbox Process the water until the sample cup is empty Click OFF the SAMPLE VALVE and SHEATH VALVE checkboxes Detach the sample tubing and remove cap from primary sample cup Fill primary sample cup with approximately Bleach solution refer to Section 8 5 5 of the COPAS BIOSORT Operator s Manual for bleach concentration specifications replace the cap and firmly tighten Reattach sample tubing to the primary sample cup Select the Acquire button and process the bleach for approximately 10 minutes Click OFF the SAMPLE and SHEATH VALVE checkboxes Detach the sample tubing and remove cap from primary sample cup Fill the primary sample cup with 40 mL of deionized distilled water Aspirate deionized distilled water and discard Fill primary sample cup again with 40 mL of deionized distilled water and firmly tighten the cap Re attach sample tubing to the primary sample cup Select the Acquire button and process the deionized distilled water for approximately 10 minutes Do not allow the sample cup to run dry Click STOP twice The system is now cleaned Clean external surfaces of any spillage using a water dampened lint free cloth 4 Place approximately 20 mL of BIOSORT CONTROL BEADS 310 5071 000 into the primary sample cup and cap firmly 5 Set PMTs as follows PMT Control Green Yellow Red 500 500 500 6 Connect oscilloscope to the Green PMT and adjust the
81. ing the B side of stage if necessary 9 Observe the droplet formation and verify there is no more than 1 satellite drop If a satellite drop is present it must fall within the well area If there is more than 1 satellite drop troubleshoot and repeat DROP TEST Click the DONE button which will return the view to the COPAS software main screen 10 Save the stage alignment upon exiting the program The alignment will be saved in the COPAS system software Union Biometrica Inc Confidential 17 www unionbio com Set up NOTE Move the oscilloscope lead from to as appropriate to obtain necessary data 1 Set Green and Red PMTSs to 500 on computer main screen Record voltage outputs from channel 2 on the oscilloscope 2 Open TOOLS Menu and select SELECT PMT FILTERS Change to Filter Set 2 3 Set Yellow PMT to 500 on computer main screen Record the voltage output from channel 2 on the oscilloscope 4 Replace the 488nm filter with the 514nm filter 5 Open TOOLS Menu and select SELECT PMT FILTERS Change to Filter Set 3 6 Ensure the Red and Yellow PMTs are set at 500 on the computer main screen Record voltage outputs from channel 2 on the oscilloscope 7 Replace the 514nm filter with the 488nm filter 8 Adjust red PMT on computer main screen to obtain a voltage output of 1 5 volts on the oscilloscope 9 Adjust Yellow PMT on computer main screen to obtain a voltage output of 1 5 volts on the oscilloscope
82. io com System Interconnect Drawings COPAS BIOSORT System Interconnect Top View COPAS BIOSORT System Interconnect Internal COPAS Connector Panel COPAS Fluidic Diagram Drawings matching these part numbers descriptions Immediately following this page Union Biometrica Inc Confidential www unionbio com 31 HOUSE AIR ACCIRCUIT 1 Dedicated Line AC CIRCUIT 2 Dedicated Line IBM PC DISPLAY COPAS SYSTEM RUNNING 066 0009 010 COM WINDOWS SER RS 232 CABLE 1 IEC 320 C14 Inlet LASER KEYBOARD MOUSE B10 5018 000 METAL CLAD FIBER OPTIC CABLE CONTROL ARGON LASER INCLUDES SPECTRA PHYSICS MODEL 160 SERIES AIR COOLED ION LASER INCLUDES 310 5010 001 LASER INTERFACE IEC 320 C 20 Inlet COPAS BIOSORT SYSTEM INTERCONNECT TOP VIEW LINE X STEPPER Y STEPPER WASTE PUMP STEPPER 350 5001 001 SORTER CONTROLLER CONDOR GMP130E POWER SUPPLY CORCOM 3EP1 FILTER IEC 320 C14 Inlet COMPORT CONNECTOR 350 5002 001 BULKHEAD CONNECTOR 350 5010 000 350 5009 001 PMT HV ASSY 350 5011 000 RED Laser Diode
83. l tubing for leaks and wear Run DI water as sample and check back round counts a Run control Beads and record results CVs MEAN EXT TOF GRNFL YELFL REDFL Union Biometrica Inc Confidential 29 www unionbio com Check off box when task is completed a Testsorting and record results Sorted Sample Type Drop Delay ___ Sort Width Coincidence Sort 10 events for 12 wells Record results Sort 1 event for 96 wells Record results Q Check the performance and alignment of the Reflx option Sort 20 Events in every other well for 24 wells Performance Yield Carry over Left in well Q Measure laser output of each laser He NE mw Argon mw other mw Q Measure Laser current at operating power Current amps Q Replace air filter check the performance and oil level on the air compressor Q Computer 1 Remove all old files 2 Run defragment program on the C drive 3 Conform that there is at lease 500 Megs of disk space free Q Remove waste tray and clean Q Check waste tray and diverter value alignment Q Check the performance and alignment of X Y stage Q Check the performance of twister and confirm twister alignment Q Clean outer surfaces of complete system Ifsystem is not performing to final test specifications then repair return to step 2 Q Complete Field Service Report Customer Signature Date FSE Performing PM Date Union Biometrica Inc Confidential 30 www unionb
84. lease contact the factory Set Up Union Biometrica Inc Confidential Instrument a Attach all cables and visually inspect system b Turn ON system Set power supply DC voltage output for 5 10vdc on sorter board as follows d Using voltadj place meter on power connect J12 Black to Black and Red to Orange e Adjust meter reading to 5 11 f Check C171 located below Serial Number Prom Meter must read 5 10 g Setthe mixer stepper motor drive current on the bulkhead board as follows h Place a meter across C5 on bulkhead board i Adjust DC voltage output using POTI for a meter reading of 0 250v 005v j 635nm Laser Verify switch 3 from bottom on the Laser Dipswitch SW1 located on the bulkhead board 15 ON to the left 1 670nm Laser m Verify switch 1 2 3 from bottom on the Laser Dipswitch SW1 located on the bulkhead board is ON to the left n Setthe dipswitches on the waste pump stepper motor to the following BIOSORT SELECT PLUS XL 24 36v ON ON 1P 2P ON ON F H OFF ON ACD ON ON Set the dipswitches on the two stage drivers to the following BIOSORT SELECT PLUS XL 24 36v ON 1P 2P ON F H OFF ACD ON Clean all bottles Fill sheath bottle and backwash bottle with Sheath Reagent Check the sample cup position www unionbio com 13 2 Computer Turn ON computer and install PCI BUS Ether Netcard per
85. me r 58 m ah DM5 PB7 T20UT ee 0 5 06 128 8 SRAM 110 RXO 5R 1 105 TO Y X Cie x LN SR 2 4 24 FEE X PC3 INTI NA m PB 5 7 s our 7 a 3 PC4 T0 0 22 50V 1 8 1 13 7 sy 5 E ul 1 6 y A6 25 PC7 RD Y 100K 22uF 35V x 17 8 ji m PCD 5 SR 18 29 120 57 a 5 20 Me CY7C64613 128NC poa 3 a a C pos N 5R A12 17 40 PD4 33 29 A13 128 61 PD5 7 5 Go 16 1 PB4 31 15 2 64 Po 15 2 M wre 12 15 14 207 16 Do 433 13 4 AOE OE RO 221R 28 a 7 Di 9 bi P n 3 9 1 12 5 5 10 02 10 2 1 6 433 RII Ds yr 22 PEZ sa 4 3 spa 10 7 ASEL 08 022 Da 705 Po 6 5 5 8 R22 9 Ds 04 PES 8 7 Vss D5 05 PES me EE be 56 105 es 54 PEG PAS 57 9 Burs 27 D7 16 95 PET 12 16 ci xd PE PAS 14 RD 58 102 ASEL Pae 16 15 33 51 5 6 9 BUFRST ES dd oq BSEL 38 17 O f 55 3 RDYVBSEL or 20 1 16 SAO 222 1004 51 44 BOEF ie 2 15 2218 98 595 SLWR V 3 14 RD 5R
86. must be performed Union Biometrica Inc recommends that a trained Union Biometrica representative install the system and perform most maintenance functions The connection of the Laser fiber optic cable requires special attention and should not be attempted by untrained persons In order to prevent the system from overheating ensure that the installation permits the unrestricted flow of air around the system components and confirm prior to installation that the Work Area and Facilities specifications have been met The COPAS BIOSORT should be used only by trained laboratory personnel Use of this instrument by an untrained operator could result in damage to the product or injury to the operator Other than procedures outlined in the Operator s Manual there are no other operator required electrical optical or mechanical adjustment or serviceable components Please refer to the Operator s Manual for complete Safety Warnings and Precautions Union Biometrica Inc Confidential 4 www unionbio com Shipping Checklist Form For Customers located in North America Japan COPAS BIOSORT Part Number 350 5000 000 Serial Number Quantity Description 1 Power Cord Cable a 1 Power Strip a 1 RS 232 Data Cable 1 CSM Cable ReFLX ONLY a 1 Laser Power Cord a 1 Strait Blade Laser Plug 2 Tubing Clamp a 20 ft PVC Air Tubing a 1 Threaded Barbed Fitting a 1 Slotted Screwdriver a 1 Waste Bottle 1 Vent Tubing a 1 Waste Pump
87. or performance outside of the published performance specifications D Service required to parts in the system that have been exposed to or otherwise affected by solutions or reagents not manufactured by Union Biometrica or another authorized manufacturer which causes poor or erratic performance and or shortened component life E Service performed by an unauthorized third party or F instrument refurbishing for cosmetic purposes All parts replaced under the original warranty will be warranted only until the end of the original instrument warranty Union Biometrica must receive all requests for warranty replacement or authorized distributor within thirty 30 days after the component failure It is the owner s responsibility to maintain the instrument in accordance to Union Biometrica s guidelines Union Biometrica reserves the right to change alter modify or improve any of its instruments without obligation to make corresponding changes to any instrument previously sold OR shipped All service will be rendered during Union Biometrica s normal hours of operation Repairs requested outside of normal business hours will be performed at Union Biometrica s discretion at prevailing after hour rates Such requests must be accompanied by an approved purchase order Union Biometrica Inc Confidential 3 www unionbio com The following exceptions apply 1 Consumable items including reagents and solutions pump tubing tubing are warranted to b
88. ove it this action will temporarily increase sheath pressure Observe pressures while performing this action and be sure not to exceed recommended pressures e Turn sample valve off and click the sheath valve several times opening and closing the valve Often these steps are effective in dislodging the clog and re starting the flow Use the stylus to remove any potential clogs in the flow cell and in the case that previous steps failed to remove the clog NOTE Always inspect stylus prior to inserting into the flow cell as damage may result if the stylus is bent or cut e Remove sample tubing from the flow cell taking care to not pull on the flow cell Insert the stylus into the flow cell while gently twisting If resistance is felt STOP lift the stylus approximately 2mm and insert the stylus again Repeat this as necessary until the stylus exits the flow cell without any resistance Raise and lower the stylus a few times while making small circles with the stylus at the bottom of the flow cell e Remove the stylus and reconnect the sample tubing Select the CLEAN button 2 3 times If this procedure has not eliminated the visible spray please contact factory for assistance 6 Check the drops for evidence of large worms using a microscope Union Biometrica Inc Confidential 24 www unionbio com Instrument Preparation 1 2 7 8 Installation Performed By Date Remove sample from primary sample cup
89. r moving the instrument from one location to another Remove system components from containers and move to laboratory bench where the system will be permanently located Due to the weight of the instrument 40 Kg 88 Ibs a minimum of two people is required to move the COPAS BIOSORT In order to lift the unit safely without tipping one person should be located on either side of the instrument to lift the unit by placing one hand under the front and one under the rear of the base Due to the weight of the external laser 33 6 Kg 74 lbs a minimum of two people is also required to move the unit Lift the Argon Laser System by placing one hand under the front and one under the rear of the base Connect all cables tubing and bottles according to COPAS BIOSORT System Interconnect Drawing and COPAS BIOSORT Fluidic Diagram contained in this Service Manual Optical Fiber Inspection Installation and Cleaning According to UBI Operational Instruction OI 112 Optical Fiber Inspection Installation and Cleaning Instructions Revision No 1 Last revision date 1 22 02 Purpose The purpose of this is to describe the pre installation inspection procedure the installation procedure the installation inspection procedure and the cleaning procedure of an Optical Fiber NOTE Use proper laser safety precautions at all times while performing these steps Always wear argon safety glasses Procedure 1 Pre Installation Inspection a Setthe
90. s m Xpi 8 x 96 3 XA6 4 A ER fs 9 A 12 XA 7 51 o S XAS 25 d 5 BRESET E BXALE Bui XAJ a 9 24 58 XAS bs XATO 21 XQ 14 26 23 n 23 t xo D us E XATI 74HC573 12 2 as 2 2 id e dil i 28 XA12 1 26 XAIS 26 XMCIO 2 iy EET XAT3 GND 203 ms 59 XA14 XATS 31 XATS 499R 15 20 XSWI0 1 PLo T2 27 79 c 2 q 4 6 4 ila MS XRD zc bu 22 22 s PL o1 wa HE 1 32 XPSEN CY7C199 27C512 33 XALE 215 P16 34 XLED 217 RXDO RXDO 80C320PLCC cn 32 cas 33 33 33 GND 1 inion Biometrica Inc 5 Medford Street Suite 101 02143 Sorter Controller Stage X Driver ize Document Number 5 350 5001 SCO D1 Pate Tuesday April 29 2003 heet 8 of 10 8 7 6 5 4 3 2 1 COPAS BIOSORT PAGE 8 HV3C U150A 9150 LF412 6150 38 GND U150B 152 7 cist LF412 33 E GND R155 z 51 gt AUXISIG AUXI 01518 10 0K 153 gt 33 GND LAN 5 LF444 R156 8 gt AUX2SIG AUX2 U151C 10 0K 154 gt 33 GND AN 2 LF444 R154 4
91. spected on a weekly basis as this tubing will wear out through normal usage Check the tubing for leaks specifically around tubing joints If you determine during the inspection that tubing looks worn or brittle carefully remove it and replace it with new tubing Once this has been done perform the Fluidics Check found in the Final Set Up Alignment Procedures section of this Service Manual Union Biometrica Inc Confidential 28 www unionbio com Annual Preventative Maintenance Procedure Revised 12 01 2001 A trained Union Biometrica Inc Service Engineer typically performs this Preventative Maintenance Procedure at the annual preventative maintenance visit As specified by the COPAS instrument warranty agreement this procedure will be performed by Union Biometrica Inc at the end of the one year warranty period and or at the end of a one year service contract period Union Biometrica Inc recommends that this Preventative Maintenance Procedure be performed annually to maintain optimum instrument performance Customer Site Serial numbers of all system components Check off box when task is completed Runcontrol Beads and record results at time of arrival to set base line CVs MEAN EXT TOF GRNFL YELFL REDFL Ifsystem does not meet final test specifications repair and then continue Q Replace all sample tubing Q Replace all o rings on sample cup Q Replace sample air tubing or sample air fittings Q Check al
92. tep number 3 found in the COPAS Instrument Test section of this Service Manual Union Biometrica Inc Confidential 21 www unionbio com Performance Verification 1 Place approximately 40mL of sample C elegans sample is recommended into the primary sample cup and cap firmly 2 Open the FILE Menu and select the 96serp file 3 Set the instrument sort delay and sort width a Select number of events to be sorted per well by entering the desired value between 0 and 50 000 in the Per Well field located on the main software screen b Set the Sort Delay by starting with a recommended value of 8 and adjust the setting up or down in increments of 0 5 until optimal sort results are achieved c Set the Sort Width by starting with a recommended value of 5 and adjust the setting up or down in increments of 1 0 until optimal sort results are achieved NOTE The sort width must be less than the sort delay d Ensure Coincidence Check checkbox is checked indicating it is ON if desired 4 Set the following GAINS and SORT PARAMETERS and record actual values if applicable Full Scale Gains Thresholds Actual Signal TOF 256 Signal Integral 50 EXT 256 or 512 EXT 80 100 FLUI 256 or 512 80 200 TOF Minimum FLU2 2560r512 FLU2 80 200 20 PMT Control Green Actual Yellow Actual Red Actual 500 600 N A N A 600 700
93. voltage to 1 5 volts using the PMT Control Union Biometrica Inc Confidential 20 www unionbio com 7 Connect to the Red and adjust to 1 5 volts using the Control 8 Open the TOOLS Menu and select SELECT PMT FILTERS Choose Filter Set 2 Green and Yellow and restart the external laser 9 Connect the oscilloscope to the Yellow PMT and adjust the voltage to 1 5 volts using the PMT Control 10 Record the final PMT values PMT Control Green Yellow Red 11 Open TOOLS menu and select RUN CONTROL PARTICLES 12 Adjust sample pressure to obtain 10 2 beads sec by selecting ACQUIRE to begin particle flow Collect beads for 1 minute and calculate the mean rate of the beads Total Specification Mean Rate 10 2 sec Total 60 Sample Pressure 13 Select ACQUIRE and process at least 1000 events 14 Record the Means and of the beads If not within specification troubleshoot instrument and repeat steps 2 6 through 2 8 15 Print the display screen and attach to this document Particle Lot No Expiration Date Mean Spec Spec TOF 15 25 59 0 28 54 EXT lt 11 0 FLUI 7 73 FLUI lt 20 0 FLU2 7 73 FLU2 20 0 16 Clean sample cup sample valve and flow cell following the procedure outlined under the Performance Set Up s

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