User's Guide Phast Blue - Ecologie Microbienne Lyon
Contents
1. activation process PhAST Blue STOP Stops the photo activation process at any time Between this two buttons there is a window with the indication of the elapsed working time The second row allows to program the parameters 5 1 2 Parameter configuration Intensity Set the photo activation LEDs power percentage Minimum value 10 Maximum value 100 Total time Set the total photo activation process time Minimum value 00 30 maximum value 59 59 Format minutes seconds 10 PhAST Blue 5 2 Section 2 Repeat cycles The use of these controls allows repeating the light cycle During photo activation process total time a working cycle can be repeated as many times as the total time allows LJ PhAST Program v1 0 Gem ee il pa PhotaActivation System for Tubes BET a default Ph In order to activate this Section 2 set a value different than 0 in Time OFF is the only parameter available in this situation PhAST Blue Repeat cycle Time ON Determines the Photo activate LEDs start time in a working cycle according to the set frequency and intensity Minimum value 00 00 maximum value 59 58 Format minutes seconds Repeat cycle Time OFF Determine the Photo activation LEDs time off in the working cycle Minimum value 00 00 maximum value 59 58 Format minutes seconds The addition of the two values Time ON and Time OFF must2. be equal or lower than Total Time PhAST Blue 5 3 Section 3 Frequency When the photo activation LEDs are lighting in a working cycle not only the intensity can be set but also the light can flash in shorter cycles Geniun IITTI PhAST Rs scala PhotoAzkivation System for Tubes T7 default Ph In order to activate this Section 3 set a value different than 0 in Pulse OFF is the only parameter available in this situation The use of these buttons allows to set the length and frequency of the flashing program PhAST Blue Frequency Pulse ON Determine the LED switch on pulse Minimum value 0 maximum value 100 Format 1 unit 100 milliseconds Freguency Pulse OFF Determine the LED switch off pulse Minimum value 0 maximum value 100 Format 1 unit 100 milliseconds The addition of the two values Pulse ON and Pulse OFF must be equal or lower than Time ON PhAST Blue 6 Section 4 Program transfer from to PC READ Read the program stored in the equipment and transfer it to the PC In the PC screen the stored parameters can be seen SAVE Store the PC active program in the internal memory of the equipment The active program is the one which is running while the equipment is on Once stored in the internal memory this will remain as the instrument autonomous program The active program working in the unit must be saved to
3. when the USB cable is connected In both cases the equipment will work accordingly to the previously established photo activation program Always install the micro tubs in a holder Never put the tubes in the hole without the holder The design of unit requires it to put the tube in its correct position WARNING Always keep the fan air inlet as well as the slots situated both sides of the equipment for right refrigeration cleared PhAST Blue 3 1 Autonomous mode Once switch ON the indicator LED is flashing green introduce the tubes with the holder in the proper position cover the tubes with the protective shield and push the pushbutton The unit will start timing accordingly to the program stored in the internal memory the default value is 15 minutes During this period the photo activation process of test tubes will be carried out the indicator LED will be red along the period During the process any time that the shield is open the photo activation LEDs will switch OFF automatically Closing the shield the process will continue When the photo activation time is over and after 30 seconds the indicator LED will change from red to flashing green Take the holder with test tubes out From this moment it is advisable to keep the eguipment ON while the fan is ON around 30 seconds to refrigerate the eguipment The instrument is ready for a new photo activation process 3 2 PC connected mode PC Recommended Requirements fo
4. PhAST Blue USER S GUIDE 50007606 00 PhAST Blue 1 Description PhAST Blue equipment has been developed to neutralize the DNA of dead organisms through the use of photo activation on intercalating agents The samples treated with this system only allow the detection of DNA of living cells by conventional PCR or Real Time PCR The PhAST Blue is a thermally stable equipment with a constant and uniform light dose and very easy to use The PhAST Blue allows simultaneous photo activation of 12 samples in a simple and efficient manner The PhAST Blue minimizes intra and inter laboratory variability while the negative effect of overheating on the samples is avoided 1 Indicator LED 2 Test micro tubs zone 3 Eyes protective shield 4 Air inlet 5 Air output 6 Push button PhAST Blue Main Switch Power plug USB connector 10 Test micro tubes holder 11 Position test tube PhAST Blue 2 Connection The PhAST Blue equipment is equipped with an IEC 60320 C13 plug to be connected to following power cord according to the country destination Europe 1 Li Schuko CEE 7 7 IEC 60320 C13 ir i a a UK nat BS 1363 IEC60320 C13 Apes USA Canada Japan i er NEMA 5 15P IEC60320 C13 tiit 1 Australia D AS3112 IEC60320 C13 al boom 3 Running The PhAST Blue equipment has been designed to work in autonomous mode when the USB cable is not connected or PC connection mode
5. he wrong port the programme will not communicate with the equipment and then it will be necessary to choose another port through the Configure USB button Driver installation and control software The software can be found in the website www geniul com Going to SUPPORT and DOWNLOAD After registration you can access to the program to be installed in the computer This installation must be done working as administrator Follow the steps in the installation program supplied PhAST Blue 5 Complete screen PC controlled The screen is divided in 6 separated sections as follow ali nl AT A PhAST_ PhotoActivation System for Tubes default Ph PhAST Blue 5 1 Section 1 Basic work Section 1 refers to the basic operation with the PhAST blue It allows to program the Intensity and Time process other than the 100 intensity and 15 minutes value of parameters by default in no PC mode LJ PhAST Program v1 0 Gnu GCoCOBEG Ce E CE g BE lo0 00 default Ph PhAST Blue The first row refers to the working functions This automatic information icon shows if the eyes protective shield is open or closed The unit can only work with the warning triangle erased The Basic operation accepts to change two parameters Intensity power intensity of the light and the Total working time Section 2 and 3 are disabled grey mode 5 1 1 Working functions START Starts the photo
6. maintain it If the parameters have been modified but they have not been saved they will be lost when switching OFF PhAST Blue 7 Section 5 Graphic display of program The programmed graph shows usually the total timing parameters working cycle frequency and intensity set with reference to the working program This graphic is dynamic and auto scaled automatically 8 Section 6 General functions t Configure USB Select the assigned Serial equipment Port which to be controlled Manual Allows opening this manual in the PC screen PhAST Blue Default parameters Allows returning to the initial default parameters Program READ Reads the directory profiles stored program files and transfers the selected program to the PC and shows it Program SAVE Save the active program in a programs file in the profiles directory EXIT Exit from the application PhAST Blue 5 Cleaning Disconnect the equipment from the power supply for cleaning Use a lightly moistened cloth or an antistatic cloth Do not use cleaning agents or abrasive dissolving products 6 Technical specifications Dimensions 355mm x 90mm x 83mm Weight 1 2 Kg Working temperature 5 C a 25 C Power range 100 240 VAC Frequency range 50 60 Hz Maximum power 60 W PC connectivity USB 7 Safety advice Please do not switch on the equipment if the power cord or the equipment is broken Do not open the in
7. r PhAST Blue Software 500Mhz Processor Intel Pentium Celeron AMD K6 Athlon Duron Windows XP Vista 7 384MB RAM 20MB of free disk space 800x600 pixels resolution VGA Video Adapter Once switch ON the equipment is waiting to receive the configuration orders start or stop from the PC through the control programme previously installed see PC control software section When the START button is pushed it will start time delay accordingly to the visualised program on the screen During this period the photo activation process of test tubes indicator LED red will be carried out During delay time we will be able to monitor the remaining process time and in any case to stop the above mentioned process through STOP push button 5 PhAST Blue After finishing the refrigeration time and passed 30 seconds the red sign will turn to flashing green colour and it you will be able to take the holder with test tubes out From this moment it is advisable to keep the equipment ON while the fan is ON around 30 sec The unit is ready again for receiving new configuration orders or start 4 PC control software When USB cable is connected the PC takes control and the PhAST Blue equipment follows the installed software When running the control software the first time it is required to choose the Serial Port to control the equipment usually it corresponds to the last port shown in the active list In case of choosing t
8. strument cover electrical danger inside Do not drop liquid in the equipment Switch off furthest end of the equipment from the power point immediately Do not look at internal LEDs used for test tubes photo activation There is a potential risk for your eyes The unit has a protection shield that only permits the LEDs lighting when closed Do not use the equipment in extremely damped environment Do not cover the equipment or the fan zone if it is working PhAST Blue 8 Warranty The PhAST Blue equipment has a 12 months warranty period which starts when the final customer purchase it The warranty is void when misuse of the equipment can be proved There are no included damages or manufacturing faults caused by nocks chemist or corrosives products liquids damp or other external factors like ray fire inadequate transport In addition the warranty will not apply if the equipment has been handled repaired modified by not qualified or specifically designated personal GenlUL S L www geniul com
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