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SOFTWARE USER MANUAL

1. a Wwriteprotect V Show only active methods Uvikon Method Themosystem Method pem Tm C i i Gradient Set Temp 20 Ramp 100 1 13 810 80 00 20 40 Time min Created from Uvikon Last change l Comment Date 18 02 2005 Date 18 02 2005 from Uvikon Method polydAdT The actionlist contains the parameter Set Temp With the right mousebutton you can insert delete and append lines Action set temp the ThermoSystem is set to the temperature without any gradient Action ramp the ThermoSystem ramps from the actual temperature to the new temperature with a given gradient Reading interval is the time of measurement ticks Lagtime indicates the additional time for reading when the ramp is finished Wait wait time for settling temperature Note A set temp and a ramp action is always required LIFEPOWER Software for Manual Version 2 01 April 2006 Page 25 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL 7 Duso Teo Softee Technik Cimahi The system prompts for a name for the method to be stored whenever a new method is to be created Once the parameters for the method have been entered the method is saved by clicking on the green tick in the option tool bar write protect LIFEPOWER Software for Manual Version 2 01 April 2006 Page 26 of 43 UV
2. lec GmbH ahii Unies Technik Cin 4 4 Query You can filter the database with the query function Select an aquisition 01 12 2000 06 12 2000 4 5 Data conversion to EXCEL format Data files can be converted directly to Excel XLS format to be read from Excel spreadsheet programs Excel must be installed for this function to be used The data will be transferred directly to Excel and can then only be saved and processed within Excel Caution This data cannot be re imported into the Lifepower software and is not subject to any further controls by the Lifepower software with respect to security considerations for electronic documentation Click on the icon to transfer data to Excel Excel is opened automatically and the data entered in a worksheet 4 6 Export to Clipboard Data files in graphical format can be exported to the Windows Clipboard to be immediately imported into other running programs via the copy paste mechanism Click on the clipboard icon in the graphics toolbar and the curve is exported automatically to the clipboard for further processing LIFEPOWER Software for Manual Version 2 01 April 2006 Page 20 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Tee GmbH 5 3D Data Acquisition new feature in the nucleic acid thermal analysis is the 30 SCAN where the experiment is executed in spectrum scan mode generally between 220 nm and 3
3. 7 6 Export to Clipboard Data files in graph format can be exported to the Windows Clipboard to be immediately imported into other running programs via the copy paste mechanism 3 Click on the clipboard icon in the graphics toolbar and the curve is exported automatically to the clipboard for further processing LIFEPOWER Software for Manual Version 2 01 April 2006 Page 32 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Teo 8 Options The optionwindow contains some additional information for documentation like serialnumber of UVIKON and ThermoSystem company labor and department Database path shows where the database is located Autosave forces to open the save dialog at the beginning and automatic save at the end of a measurement If you logged in with administrator rights the checkbox View all measurements enables you to see all measurements of all users otherwise only your measurements are shown If you are logged in with user rights only your measurements are shown Waiting conditions are shown in the section ThermoSystem The start message Wait for temperature reached in cell indicates that the program waits until the temperature of the sample cell has the correct value The start message Wait for temperature reached in ThermoSystem indicates that the program waits until the temperature of both thermocells has the correct value The temperature is calculated w
4. lings Softens Fesfwik cmi 9 Direct Printing of Curves Clicking on the Chart tab in the Report screens of modes such as Wavelength Scan gives the user a direct full screen display of the curves corresponding to the selected report The appearance of the curve may be modified using the Edit function which is described in a separate section below E Prints the curve as a single sheet report Click on this icon to obtain the formatting window below This method will only print the curve itself TeeChart Print Preview 2 OF x Printer Defaut Frinter Print Orientation Portrait Landscape Reset v View Margins Proportional The upper bar in this dialogue box controls current operation and selection of the printer parameters using the standard printer installation windows The left hand section of the window contains formatting functions which directly influence the print out itself e g margins detail etc Changes to these parameters directly influence the appearance of the print out preview in the dialog window These functions are explained briefly below Orientation Selection of either landscape horizontal or portrait vertical format for the print out Margins opin selection boxes allow direct setting of the individual page margins Reset Margins Changing the preset margins automatically activates the Reset Margins button click on this
5. methods and curve data Each window contains its own Printer icon which gives a preview of the print out Within the preview page the user also has the possibility to export reports as PDF or Word zn wm ie im Printer BAET n 48434 C 1 e L 1 i fo mr 0 1 1 1 1 1 1 1 1 1 233 Irii mA caci dy bii mi Mac 1 rm pH Enn LIFEPOWER Software for Manual Version 2 01 April 2006 Page 37 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Tee Softee Technik Cali i 11 Editing and Formatting of Charts The software uses a built in editor which offers comprehensive formatting capabilities for both 2D and 3D reports Access to this editor is obtained by clicking on the Edit icon This icon is available in all screens including the Acquisition screen Use of the Edit facility when programming a method allows immediate control of the appearance of the result without the need for time consuming manual manipulations A Help function is provided within the Editor and therefore this document will only describe the more common needs with respect to formatting of charts the user is referred to the Help function for additional information 11 1 Changing the
6. 3 4 STS CO EE 16 4 3 5 mcis 019 f ses NOCT NN ace sie E 19 o DEN ed 20 4 5 Data conversion to EXCEL 20 s eo cox cosi RN 20 SMEMEC B Bir 21 5 1 Prepare an aquisition pee 21 5 1 1 vere 1 111 6 1o E E RR 21 I NO E 23 6 1 Create new siio aene uina duin cu SUN o atin Du ER Ea UR aH 24 6 1 1 liaec e e S 25 ANE Dr gt TE E 27 eg cok gtd a oe te ere es A 28 l2 Chancommander 28 OU 30 TA 20 22 2 m 31 7 4 1 Scan by given temp 31 742 Temperature by given 31 75 Data conversion to EXCEL 32 EPO
7. at any time to reset all margins to their default values The button is not active if the margins are already set to their default values LIFEPOWER Software for Manual Version 2 01 April 2006 Page 35 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL AT Se Tes GmbH vae Tomik Cali d J View Margins This checkbox allows margin display in preview to be switched on or off as required Detail Moving the slider between normal and more increases the degree of detail shown in the curve display print out For example for greater detail on the axis display move the cursor towards more until the required detail is displayed Proportional This returns the print out to its original default settings without affecting the detail selected in the detail scroll bar LIFEPOWER Software for Manual Version 2 01 April 2006 Page 36 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Tee Softee 10 REPORTING This section deals only with the possibilities for formatting and printing reports in the Lifepower software The main focus is on the treatment of curves since all table format printouts are pre defined 10 1 Printing The Lifepower software offers several possibilities for printing and formatting the various reports and data available from the system Individual print outs are possible for method parameters individual results e g gradient data
8. for Manual Version 2 01 April 2006 Page 28 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Tee Softee Click on this icon to move complete curve display using mouse cursor Clicking on this d icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Click on this icon to rotate the complete curve display using the mouse cursor Clicking on this icon automatically converts the display format to 3D return to the 2D display the 3D icon button Clicking on this icon will return the function of the mouse cursor to normal thus allowing use of the 4 normal zooming functions etc in 3D displays LIFEPOWER Software for Manual Version 2 01 April 2006 Page 29 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL 7 3 Query You can filter the database with the query function Select an aquisition LIFEPOWER Software for Manual Version 2 01 April 2006 Page 30 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL 7 Duso Tee ings Sofas Fesfui d 7 4 2D View of 3D experiment With the button an additional windows opens This window contains 2d scan curve at a given temperature or a 2d temperature curve at a given wavelength Note If in option the z axis is set to sequential the selected scan or temperature is shown in the original 3 D graphic 7 4 1 Scan b
9. the right while keeping the left button pressed A rectangle will be shown on the screen indicating the area that will be magnified When you reach the lower right corner of your area release the button and the area selected is zoomed to fill the graph area You can repeat the zooming function up to the maximum resolution by repeating the selection of the zoomed area To unzoom simply position the cursor on the graph press the left mouse key move the cursor to the left and release the left mouse key or double click with left mouse key 7 2 Chartcommander Debie The chart commander allows to change the view of the charts Copy to clipboard print 3D view zooming and panning are possible Exports a curve to the Windows clipboard for further processing Prints the curve as a single sheet report Formatting of the report is described in a separate section of this document dealing with curve format Opens the edit dialogue for formatting functions See the separate section of this document dealing with curve format for more details 3p Converts chart format to a 3D display The icon functions as a toggle switch between 2D and 3D display Click on this icon to control the depth of the 3D display using the mouse cursor Clicking on this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button function icon allowing zooming of the complete curve LIFEPOWER Software
10. with sampling interval 0 25 nm the max scan speed is 500 nm min with sampling interval 0 5 nm the max scan speed is 1000 nm min with sampling interval 1 nm the max scan speed is 2000 nm min with sampling interval 2 nm the max scan speed is 2000 nm min with sampling interval 2 5 nm the max scan speed is 5000 nm min with sampling interval 5 nm the max scan speed is 5000 nm min with sampling interval 10 nm the max scan speed is 5000 nm min See UVIKON manual for further information Important Note Lifepower acquires temperature data directly in your sample using the unique UVIKON temperature sensor This setup allows very accurate collection of temperature data of your actual sample When setting your ramp temperature in the software package please remember that the temperature control of the ThermoSystem is done by the ThermoSystem controller The ThermoSystem controller does not get feedback from the cell temperature sensor Therefore there will be a difference between the measured temperature in the sample and the indicated temperature of the Thermosystem controller The temperature of the Thermosystem controller is given by the temperature gradient setting in your software package shown in the field ThermoSystem Sample Reference The temperature of the cell content is recorded in the diagrams and shown in the field UVIKON Cell Temp For best results we recommend to set a higher or a lower temperature in the Thermosystem method of your software package
11. 30 nm at regular user preset time or temperature interval until the final temperature has been reached The raw or baseline corrected data are displayed in 3D mode Abs wavelength temperature It is also possible to obtain a 2D Abs temperature thermal transition curve at any wavelength 3D Scan is particularly useful at the beginning of a new research project since it helps to detect whether the melting process involves only two or even more components For the complicated reactions where there are more than one equilibrium it is possible to identify the component by spectral deconvolution and separate different thermal transitions by setting the appropriate wavelength isobestic point 5 1 Prepare an aquisition Select a method selcting the method combo box 3D Acquisition Gradient Uvikon Sample 1 Cell Temp C Cell Temperature Absorbance Wavelength Absorbance Wavelength nm Thermosystem Sample C Reference C Te m pe ratu re of thermosystem Edit Sample Reference C Settings of thermosystem 220 225 230 235 240 245 250 255 260 265 270 275 280 285 290 295 300 305 310 315 320 325 330 Aquisition control Method LNA3D2 gt Start Run Baseline User Samples 1 Sample Time 0 00 06 Remaining Time 0 00 Action Finished Method combo box Acquisition control Fill the samples into the sampleholder Choose your method by selcting the method combo box Choose the number
12. 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Tee Softee Technik Cali i 2 2D Data Acquisition The basic feature in the nucleic acid thermal analysis is the 2D Data Acquisition where the experiment is executed in fixed wavelength wavelength program mode generally between 220 nm and 330 nm and up to 6 selectable wavelengths at regular user preset time or temperature interval until the final temperature has been reached The raw or baseline corrected data are displayed in 2D mode Abs temperature with different color curves for each wavelength in one diagram 2 1 Prepare an aquisition Select a method by selecting the method combo box 2D Acquisition 3 Values Result Description Gradient um Absorbance Absorbance wavelength Wavelength Thermosystem Sample C Temperature of Reference C the rm osystem Edit Sample 00 Reference C _ Settings of thermosystem iiss Aquisition control Basepoint gt start Run Baseline User Samples Sample T ining Ti Action Method combo box Basepoint field Acquisition control 2 1 2 Basepoint If you want to measure a basepoint insert the blank sample in sampleholder 1 and select Measure Basepoint in the basepoint field Start the aquisition with the start button at the aquisit
13. IKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso ings Todi 7 3D Report The Report shows all previous stored data Select the data in the database tabsheet Note The view of the database depends on the login name of windows Only those data are shown which has the same username as the login name of windows In the scan display the scans are shown in graphical format In multi window mode one sample is displayed in one chart with all temperature curves With double clicking on one chart this chart will be expanded to full window Second double click will reverse this function Report 30 v Protected pi e Database Gradient ap Sample 1 Sample 2 Sample 3 29R 36716 SOR Y Mus T15 p o 4 sample 4 29R Y 5 ZA pepeg LT i Sample 7 ZUR 1B5TO Bos m s co za a Bu Hae DEMO LIFEPOWER Software for Manual Version 2 01 April 2006 Page 27 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Tee GmbH Softee Technik Cali i 7 1 Zooming Simply move the cursor on the graph to the upper left corner of the rectangle to be magnified press the left button of the mouse and move it to
14. LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL NH AT Duso Teo lings Softee Fesfwik cmi SOFTWARE USER MANUAL 1 1 1 1 1 1 1 1 1 T 1 1 tT 1 Lifepower Software for UVIKON XL LIFEPOWER Software for Manual Version 2 01 April 2006 Page 1 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL 3 2 2D 0 re an ene A T M m 4 2 1 Prepare ACQUISIMION REDDE mE 4 ZR eae 4 2 1 2 610 g a eee eee eee ee eee ee eee eee 4 As ACU ON e 5 peo Pen ee 6 3 1 Create a new 7 3 1 1 TM TUNA SSO ETT 8 A Do T 10 AE 200 m 11 A2 Given eH EE Om Oe 11 4 3 Lifepower 13 4 3 1 BIST 14 4 3 2 itio E NETTE m 14 MEME 16 15 4
15. N CPOO 6 CR E 32 ee ee ee 33 S or eee eee ee eee ee 33 ec 34 9 Direct Printing ee ee eee ee 35 10 Bi 37 10 1 ne er eee E E E 37 11 Editing and Formatting Of ERE m 38 11 1 Changing TAS Scalig AMIS 2 38 e 38 n FOMA EET E 39 INE NEU ce ances 39 INT TUOS ea eE A dice greene ue cnn ence ee dest uote eee E 40 FERS CTI 40 41 12 CANA E 42 13 IT POUDIO S NOOU 43 LIFEPOWER Software for Manual Version 2 01 April 2006 Page 2 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL YH AT Duso Tee 1 introduction Melting Temperature Tm of Nucleic Acids Lifepower is a software program designed to acquire and store data for the evaluation of the meltpoint of a nucleic acid sample Data generated with this softw
16. are package can be saved printed or exported in ASCII or EXCEL format to be used for further calculations Abs versus temperature data with autoscaled graphs and real time data storage are provided User defined comments are stored within the data file and all entered parameters are saved as a customized command file or as default parameters for the next experiment Once the thermal experiment starts Lifepower continuously acquires the Abs and the temperatures at a regular user preset time or temperature interval until the final temperature has been reached Regular time interval should be preferred because it allows further kinetic analysis Real time graphics are automatically displayed and updated during data acquisition At the end of the experiment the displayed raw data by default can be proceeded and normalized The melting temperature Tm value and the shape of the thermal transition depends on the sequence and length of the nucleic acids as well as experimental conditions ionic strength pH etc The Tm value is a transition point where the complex is half dissociated or half formed This transition point corresponds approximately to the inflection point of the melting curve where the slope becomes steepest DNA thermal melting curve will provide with not only the melting temperature Tm but also the free energy and its associated enthalpy and entropy contributions LIFEPOWER Software for Manual Version 2 01 April 2006 Page 3 of
17. display format to 3D return to the 2D display via the 3D icon button Clicking on this icon will return the function of the mouse cursor to normal thus allowing use of the normal zooming functions etc in 3D displays LIFEPOWER Software for Manual Version 2 01 April 2006 Page 12 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Teo Limes Softee Technik Cimahi 4 3 Lifepower calculations In the chart view you can calculate the TM Point by pressing the calculate button Click on this icon to open the calculation dialogue window The edit calculation paramter screen allows definition of the calculation modes Edit Calculation Parameter Function j Curve function Show arrow 1 1 2 Show derivative ET ug Detect Show label Detection scheme Peakdetection Smooth None Average Basepoint Load Basepaint Clear Bezier The required number calculations to be used must first be defined using the spin box at the top of the window This definition will open the appropriate number of lines where the actual values can be entered directly Calculation Wavelengths are defined for calculation purposes as W n or w n respectively where corresponds to actual number for a given wavelength as defined in the method In the example above the following calculations will be performed Wavelength number 1 e g at 400
18. e Lifepower acquires temperature data directly in your sample using the unique UVIKON temperature sensor This setup allows very accurate collection of temperature data of your actual sample When setting your ramp temperature in the software package please remember that the temperature control of the ThermoSystem is done by the ThermoSystem controller The ThermoSystem controller does not get feedback from the cell temperature sensor Therefore there will be a difference between the measured temperature in the sample and the indicated temperature of the Thermosystem controller The temperature of the Thermosystem controller is given by the temperature gradient setting in your software package shown in the field ThermoSystem Sample Reference The temperature of the cell content is recorded in the diagrams and shown in the field UVIKON Cell Temp For best results we recommend to set a higher or a lower temperature in the Thermosystem method of your software package than you actually want to achieve in your sample The current temperature in the sample will always be lower than the Thermosystem temperature display when increasing and higher when decreasing the temperature of the hardware LIFEPOWER Software for Manual Version 2 01 April 2006 Page 6 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Camb I ings Softee Technik Chibi 3 1 Create a new method Disable the write protect checkbox and press the button fo
19. e of Windows Only those data are shown that has the same username as the login name of Windows All raw values will be shown in the value grid If you load a basepoint the results will be shown in the result grid With right mouse button you can copy the values to the clipboard In the chart display the results are shown in graphical format with different colors for each sample You can choose between multi window and overlay window In multi window mode one sample is displayed in one chart with all temperature curves With double clicking on one chart this chart will be expanded to full window Second double click will reverse this function In the scan snapshot display the scans are shown in graphical format with different colors for each sample You can choose between multi window and overlay window In multi window mode one sample is displayed in one chart with all temperature curves With double clicking on one chart this chart will be expanded to full window Second double click will reverse this function Report 2D S 4 gt i v Protected rib B Database Gradient Values Result Chart Scan Snapshot Username Parameter 20 09 2005 Method 20 09 2005 rosaliet 20 09 2005 19 12 2000 20 07 2004 Administrator 21 07 2004 21 07 2004 27 07 2004 27 07 2004 27 07 2004 28 07 2004 28 07 2004 29 07 2004 19 09 2005 01 07 2005 Wilma TestVorlauf 20 07 2004 Mame DEMO Administra
20. ge 14 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL N Duso Teo GmbH Softee Technik Cab d Sample 2 400 400 nm Absorbance c n 54 5B 58 60 B2 54 Ba T0 Temp C 4 3 3 Smoothing A filter can be introduced in the data acquisition in order to reduce noise Smoothing can be selected as an averaging or a Bezier function High smoothing will reduce noise however high smoothing will also reduce the amplitude of the peaks obtained and can also result in distortion of peak shape Average This is a rolling average function based on 3 calculated points Only Y values are calculated Bezier A Bezier curve is an approximation to a curve which would pass through all the control points The method used here for defining such a curve approximation is called the Bezier Bernstein approximation LIFEPOWER Software for Manual Version 2 01 April 2006 Page 15 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL 4 3 4 Detection of TM Duso Tee Softee Technik Cali i This calculation mode can be used to show the TM point The detection is made through the derivate Curve Threshold Detection scheme Peakdetection Max Value Left bound Right bound fz Edit Calculation Parameter Curve function Show arrow Show derivative Expand derivative Detect TM Show label Treshold Left b
21. he parameter Uvikon method Themosystem Method No Action Temp Time Gradient min With the right mousebutton you can insert delete and append actions Action set temp the ThermoSystem is set to the temperature without any gradient Action ramp the ThermoSystem ramps from the actual temperature to the new temperature with a given gradient Reading interval is the time of measurement ticks Lagtime indicates the additional time for reading when the ramp is finished Snapshot scan a scan will be measured with the parameter of UVIKON method Wait wait time for settling temperature Note A set temp and a ramp action is always required LIFEPOWER Software for Manual Version 2 01 April 2006 Page 8 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL N Duso Tee Limes Softee Technik Cimahi The system prompts for a name for the method to be stored whenever a new method is to be created Pe Once the parameters for the method have been entered the method is saved by clicking on the green tick in the option tool bar write protect LIFEPOWER Software for Manual Version 2 01 April 2006 Page 9 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Tee x oleh 4 2D Report The Report shows all previous stored data Select the data in the database tabsheet Note The view of the database depends on the login nam
22. ion control field The values are shown in the basepoint grid LIFEPOWER Software for Manual Version 2 01 April 2006 Page 4 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Gmbh vae Tomik J Save the values with save button window shows prior saved basepoint names Note only those names are shown which has the same method If you select Load Basepoint a prior saved basepoint will be shown in the basepoint grid All the measured values will be substracted by the basepoint With No Basepoint no calculation will be made 2 1 3 Acquisition Fill the samples into the sampleholder Choose your method by selecting the method combo boxand your number of samples Start the acquisition with the start button All raw values will be shown in the value grid If you choose a basepoint the results will be shown in the result grid Using the right mouse button you can copy the values to the clipboard In the chart display the results are shown in graphical format with different colors for each sample You can choose between multi window and overlay window In multi window mode one sample is displayed in one chart with all temperature curves With double clicking on a chart this chart will be expanded to full window Second double click will reverse this function If the measurement was crashed down the Load last measurement is enabled after starti
23. ith the first temperature in the actionlist the tolerance 8 1 3D With the Z axis button you can select between temperature or sequential Z axis in the 3D chart Sequential means the z axis shows the number of scans in ascending order Select the view between lines and surface The line view allocates less memory and is faster than the surface view Display scan You can set the resolution to a lower value by increasing the number for display every points of scan and the number of every scan Example every points of scan 2 every second point will be displayed every scan 2 every second scan will be displayed means the resolution was 4 time lower as normal But all points will be saved Setting the resolution of the 3d view to a lower value increases the speed and less memory is allocated Important Note If you have trouble with speed and low memory please use line view and set the resolution to a lower value LIFEPOWER Software for Manual Version 2 01 April 2006 Page 33 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Nu Duso Teo 8 2 Chart Control Chart color Color table for curve colors in the order in which the curves are shown The colors may be selected individually by clicking on the grid Option General Thermosystem aD Chart Color LIFEPOWER Software for Manual Version 2 01 April 2006 Page 34 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL 7 Duso Tee
24. llation Connect the UVIKON with the serial data cable to com1 and the ThermoSystem with the serial cable to com2 Install the software with the setup procedure stored on CD Follow the instructions of the setup LIFEPOWER Software for Manual Version 2 01 April 2006 Page 42 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL AT Gmbh vae Tomik Cali d UO 13 Trouble shooting e Message UVIKON not found Please check the serial connection Uvikon has to be connected to com1 Message ThermoSystem not found Please check the serial connection The thermosystem has to be connected to com2 Please check also the options for com settings LIFEPOWER Software for Manual Version 2 01 April 2006 Page 43 of 43 UVIKON XL
25. mouse key 4 2 Chartcommander n amp 5 0 LIFEPOWER Software for Manual Version 2 01 April 2006 Page 11 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL YH AT Se Tes GmbH vae Tomik Cali d J The chart commander allows to change the view of the charts Copy to clipboard print 3D view zooming and panning are possible Exports a curve to the Windows clipboard for further processing Prints the curve as a single sheet report Formatting of the report is described in a separate section of this document dealing with curve format Opens edit dialogue for formatting functions See the separate section of this document a dealing with curve format for more details 3D Converts chart format to a 3D display The icon functions as a toggle switch between a 2D and 3D display Click on this icon to control the depth of the 3D display using the mouse cursor Clicking on this icon automatically converts the display format to 3D return to the 2D display via the 3D icon button Cj function icon allowing zooming of the complete curve Click on this icon to move the complete curve display using the mouse cursor Clicking on this icon automatically converts the display format to 3D return to the 2D display the 3D icon button Click on this icon to rotate the complete curve display using the mouse cursor Clicking on this a gt icon automatically converts the
26. ng the software again All data will be recovered and the system starts at the last point if you press this button and start again the measurement LIFEPOWER Software for Manual Version 2 01 April 2006 Page 5 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL YH Gmbh vae Tomik Cali d J 3 2D Method The method window shows all relevant parameters for instrument and ThermoSystem A method shows the used indicator if you have saved a measurement with the corresponding method You can only delete or edit used methods if there is no associated measurement with this method anymore Parameter interlocks of uvikon instrument Averaging time must be greater than Response time Snapshot scan There is a cross limitation between Sampling interval and Scan speed with sampling interval 0 05 nm the max scan speed is 100 nm min with sampling interval 0 1 nm the max scan speed is 200 nm min with sampling interval 0 25 nm the max scan speed is 500 nm min with sampling interval 0 5 nm the max scan speed is 1000 nm min with sampling interval 1 nm the max scan speed is 2000 nm min with sampling interval 2 nm the max scan speed is 2000 nm min with sampling interval 2 5 nm the max scan speed is 5000 nm min with sampling interval 5 nm the max scan speed is 5000 nm min with sampling interval 10 nm the max scan speed is 5000 nm min See UVIKON manual for further information Important Not
27. nm is added to Wavelength 2 Other operators 4 sqr sqrt exp In may be entered LIFEPOWER Software for UVIKON XL Manual Version 2 01 April 2006 Page 13 of 43 LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL AT Teo Addition W1 W2 Subtraction W1 W2 ERN W1 W2 Wm oir oquare root sqrt W1 mo Logarithmus naturalis Ln W1 Decimals The number of decimal places to be used for the calculation can also be defined This number should generally be consistent with the actual measured result e g by measurements to 4 decimal places selection of a much higher number for the calculation result will not increase the accuracy of a measurement or result and could produce rounding errors Factor Each sample can have their own multiplier and divisior If in the formula is a F added then this factor is used otherwise the absorbance will be multiplied with this factor Example W1 W2 F 4 3 1 Derivative The software allows direct calculation of first derivative order To display a derivative click on the Show derivative checkbox The curve display will automatically change to show the new derivative curve 4 3 2 Show arrow Selecting the Show arrow parameter will display each data point collected on the curve as shown in the zoomed example below This can help to identify a backward experiment LIFEPOWER Software for Manual Version 2 01 April 2006 Pa
28. of samples Start the acquisition with the start button In the chart display the scans are shown in graphical format With double clicking on one chart this chart will be expanded to full window Second double click will reverse this function If the Measurement was crashed down the last measurement is enabled after starting the software LIFEPOWER Software for Manual Version 2 01 April 2006 Page 21 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Tee Limes Softee Technik Cimahi again All data will be recovered and the system starts at the last point if you press this button and start again the measurement LIFEPOWER Software for Manual Version 2 01 April 2006 Page 22 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL YH Soleo GmbH vae Tomik J 6 3D Method The method window shows all relevant parameters for UVIKON instrument and ThermoSystem A method shows the used indicator if you have saved a measurement with the corresponding method You can only delete or edit used methods if there is no measurement with this method anymore Uvikon instrument Parameter interlocks Averaging time must be greater than Response time Snapshot scan There is a cross limitation between Sampling interval and Scan speed with sampling interval 0 05 nm the max scan speed is 100 nm min with sampling interval 0 1 nm the max scan speed is 200 nm min
29. ound Right bound Detection scheme Peakdetection v Smooth 2 None Average Bezier LIFEPOWER Software for UVIKON XL The threshold is the difference in height on the Y axis of the leading and trailing edge If the difference is lower than the set threshold then the TM will not be detected Use this option if a backward experiment is saved and there exists more than one TM point This allows to show more than one TM label If there is only one TM point use this option to identify exact one TM point in the curve Define the bounds within the TM will be calculated Manual Version 2 01 April 2006 Page 16 of 43 LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Tee GmbH Things uc Chi d With this parameters you will get 2 TM points in the example shown below ise Report 2D a Protected pi 3 Chart Select E w Chart Sample 1 55 c c ae mm E Ehara Multivvindoyy 50 55 Temp C O Overlay window All parameters are easy to find except Threshold but how do you get this parameter Please uncheck the checkbox Expand derivate because Lifepower calculates the TM point with the original derivate LIFEPOWER Software for Manual Version 2 01 April 2006 Page 17 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Teo lings Softens Fesfwik Chie i Lifepowe
30. r a new method Fill the fields with the values corresponding to your experiment Method BEE wwriteprotect v Show only active methods Uvikon Method Method Method Used Not Active Snapshot Scan Wavelength Common parameter Bs lr 20 100 C ScanHigh No of Wavelength Lampchange Scan Low 220 Scaninterval 1 Fitterchange 1 Scanspeed 500 Ein Filterchange 2 01 Autozero at stat Fitterchange 3 Filterchange 4 Filterchange 5 Bandwidth Delay Average 1 Response Time gt Sampler mode Created from Uvikon Date 1 8 02 2005 Last change from Uvikon Date 1 8 02 2005 Method polydAdT LIFEPOWER Software for Manual Version 2 01 April 2006 Page 7 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Teo ings Teche 3 1 1 Thermosystem Method ERES E gt wwriteprotect V Show only active methods Uvikon Method Themosystem Method Action LU ET i i i i i i SS i i i Gradient W ail Set Temp Ramp Set Temp 40 Time min Created from Uvikon Date 18 02 2005 Last change from Uvikon Date 18 02 2005 Method polydAdT The actionlist contains t
31. r avarage the first 10 points of derivate to eliminate noise and take this as the basepoint for further calulations The red line in the example This basepoint has the value 0 5625 Pratected ti Um 1 1 Database Gradient Values Chart C Cursor Select v Chart Sample 1 Absorbance Charts o Q Overlay window p LIFEPOWER Software for Manual Version 2 01 April 2006 Page 18 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Tee GmbH Cite Sofas The maximum of the derivate curve is around 0 5715 so the differenz is 0 009 C Protected ri E xm Database Gradient values Chart Select Chart Absorbance charts gt Mulitiveindov Overlay indos If you the Threshold to 0 005 Lifepower will find all peaks with a maximum greater than 0 005 In this example Lifepower find two TM points because the maximum of derivate is 0 009 Lifepower will find a TM point if the leading edge is greater than Threshold and the trailing edge is below 50 of Threshold Protected Database Gradient falues Chart C Sample 1 Charts 97 Muttivvindowys window LIFEPOWER Software for Manual Version 2 01 April 2006 Page 19 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL 7
32. rt the chart Maximum If automatic is set to off a fixed signal maximum can be defined here Minimum If automatic is set to off a fixed signal minimum can be defined here Desired increment The desired axis increment is the minimum step between axis labels It must be either a positive number or DateTime value This value will be used as the initial step value If there is not enough space for all labels then a larger increment will be automatically calculated This value defines only the increment used on the scale it is also possible to format the number of decimal places required as described below Logarithmic Check this box to obtain a chart based on the corresponding logarithm 11 1 2Decimal Format The decimal format required can also be set from within the Axis tab Click on Labels and then on the Format tab to obtain the following window A xl Ass Tiles Legend Panel walls 30 v Visible Scales Tile Labele Ticks Minor Position Behind Ames Style Format Text Exponential Values Format 0 0000 v Default Alignment Enter the required decimal format in the Values Format box e g for 4 decimal places enter 0 0000 11 1 3Title This allows formatting of the name to be used for a given axis e g Absorbance The tabs allow definition of the name its alignment relative to the axis and also the font and test appearance to be used LIFEPOWER Sof
33. s Tiles Legend Panel walls 30 Style Position Symbols Format Text Gradient Shadow Visible Legend 5tule Automatic Inverted Text Style Let Value v Check boxes Spacing 0 ind Font Series Calor The Style and Position tabs are most likely to be used The remaining tabs control items such as size and form of the displayed symbol and generally the applied default values will not need changing 11 1 6Style Visible Inverted Checkboxes Legend Style Text style Vert Spacing Dividing Lines LIFEPOWER Software for UVIKON XL Unchecking this checkbox will remove the legend box from the chart Checking this box will cause the legend order to invert i e the last sample to run will be shown at the top of the box Unchecking this box will remove the checkboxes adjacent to the text in the box This is not recommended since the checkbox is very useful if a single curve should be removed from the overlay for any reason It is recommended to maintain this setting as Automatic per default It is recommended to maintain this setting as Left Value per default Controls the line spacing used within the legend box Allows definition of the format of lines used between the individual legend entries within the box Manual Version 2 01 April 2006 Page 41 of 43 LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Gmbh vae Tomik 12 Insta
34. scaling Axis The axis scaling is the first available tab in the Edit dialogue box when opened Separate parameters can be defined for each axis and normally users would only be concerned with the so called left and bottom axes Y and X respectively A1 x Axis Titles Legend Panel walls 30 v Visible Scales Title Labels Ticks Minor Position Behind Axes Automatic v Visible Inverted W Auto Change Masimum 1 545 Auto Change Minimum 0 012 Change Desired Increment 0 001 Logarithmic Right Axis Top Asis Bottom Axis Depth Asis The required axis to be formatted is selected by clicking in the list in the left hand panel Above this panel there is also a checkbox which allows the axes to be removed from the chart visible or not The significance of the other parameters is described briefly below 11 1 1Scales Automatic The software uses dynamic scaling depending on the level of the signal obtained In many cases this can be confusing for the user and generally it is recommended that once known the range should be set manually for routine operation LIFEPOWER Software for Manual Version 2 01 April 2006 Page 38 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL 7 Teo Visible Check this box if you want an axis to be visible uncheck to remove it Invert Checking this box will inve
35. than you actually want to achieve in your sample The actual temperature in the sample will always be lower than the Thermosystem temperature display when increasing and higher when decreasing the temperature of the hardware LIFEPOWER Software for Manual Version 2 01 April 2006 Page 23 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Teo niis Sofas Teslenik Clie 1 6 1 Create a new method Disable the write protect checkbox and press the button for a new method Fill the fields with the values corresponding to your experiment Method ad gt H Show only active methods Livikon Method Themosystem Method Methad Used Not Active gt snapshot Scan parameter scan High 330 Lampchange 338 Scan Lowe 220 Scaninterval 1 b Fiterchange 1 Scanspeed 500 i Fiterchange 2 Autozero at start Fitterchange 3 4 Fiterchange 5 Bandwidth 20 reduce Delay Average Response Time 041 Sampler mode mem Created gue Comment from Ibiophymember Date 15 06 2001 Last change from biophymember Date 15 05 2001 Method 2 LIFEPOWER Software for Manual Version 2 01 April 2006 Page 24 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Teo ings Teche I 6 1 1 Thermosystem Method 3 4 gt
36. tor Administrator Administrator Administrator Administrator Administrator Administrator Administrator Administrator LIFEPOWER Software for UVIKON XL Experiment ID rosalie1ffff SS 26RUI26 Y A 18TCG23 26RU 26y44 18TCS Remarks 10 mM cacodylate 100 mM NaCl 10 mM pH 5 9 Manual Version 2 01 April 2006 Page 10 of 43 LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Duso Teo Chis Limes Seo Pss TU Report 2D u a e n Eme mE Database Gradient Values Chart Select Sample 1 ies v Chart o c G Q i 40 45 50 55 60 Temp C Sample 2 Charts Muttivindow Temp O Overlay window Mame test ctDNA polydAdT 4 1 Zooming Simply move the cursor on the graph to the upper left corner of the rectangle to be magnified press the left button of the mouse and move it to the right while keeping the left button pressed A rectangle will be shown on the screen indicating the area that will be magnified When you reach the lower right corner of your area release the button and the area selected is zoomed to fill the graph area You can repeat the zooming function up to the maximum resolution by repeating the selection of the zoomed area To unzoom simply position the cursor on the graph press the left mouse key move the cursor to the left and release the left mouse key or double click with left
37. tware for Manual Version 2 01 April 2006 Page 39 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL N 11 1 4Titles Normally only the definition of a chart titles in the form of either header or footer text is required This can be done by selecting the required function using the drop down menu and entering the text in the lower panel Anis Titles Legena Panel Walls 3D Tite Style Position Format Test Gradient Shadow 4 Visible AdjustFrame Alignment C Lett Center Right Text WAVELENGTH SCAN DOCUMENTATION Alignment top or bottom is dependent on this selection while the horizontal position can be controlled directly from the Alignment box A custom alignment using coordinates is also possible by clicking on the Position tab The remaining tabs are used to define the font type colors frame and certain special effects such as shadowing etc 11 1 5Legend The legend box is used to show which curve colour corresponds to a particular sample The default text used is Series N where N is the number of the sample in the batch This default text is only used when no sample description is defined otherwise the actual description will be used automatically for the legend LIFEPOWER Software for Manual Version 2 01 April 2006 Page 40 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL Yh dT Duso Teo Softee Technik Cimahi 3 xl Avi
38. y given tem y 9 2D Scan Select the temperature gradient to show scan ezan kd u 2 e u Em SaO o K 0 220 225 230 235 240 245 250 255 260 265 270 275 280 285 290 295 300 305 310 315 320 325 330 nm BH Temp 101 o E Temperature gradient VETE Eu E gt 20 20 28 50 26 0 294 G4 00 05 0 20 2 he 7 4 2 Temperature by given scan Select the slidebar to show the corresponding temperature Hapon ieit Sample 2 29RY J436T16 0 SaL LIFEPOWER Software for Manual Version 2 01 April 2006 Page 31 of 43 UVIKON XL LIFEPOWER SOFTWARE for UVIKON XL USER MANUAL AT Gmbh J Storm Technik Cimahi 7 5 Data conversion to EXCEL format Data files can be converted directly to Excel XLS format to be read from Excel spreadsheet programs Excel must be installed for this function to be used The data will be transferred directly to Excel and can then only be saved and processed within Excel Caution This data cannot be re imported into the Lifepower software and is not subject to any further controls by the Lifepower software with respect to security considerations for electronic documentation Click on the icon to transfer data to Excel Excel is opened automatically and the data entered in a 25 worksheet

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