Hybex Microarray Incubation System - User Manual
Contents
1. SciGene Corporation All rights reserved SciGene and Hybex are trademarks of SciGene Corporation Sunnyvale CA All other trademarks used in this manual are the property of their respective owners Version 1 1E August 2012 1 408 733 7337 techserv scigene com SCIGEME mec microarray Incubation System USER MANUAL Table of Contents L SAFER NOTICES ri airs cca eco sce naseanosanceecee cet vwcenec anes cosa sacsesseceacezsasseesaepeeccamnbavwauuwssacesecaheatiaceeaees 3 A Jatene WR E 3 WSU LVS i ee eegen 3 Ge SVM OlS aNd CONVENTIONS EE 3 DE WSS E 4 E CUON eee eee ee eee ene eee ee ee eee ee ee ee ee eee eee 4 F e ei ee e EE 4 i UNPACKING AND SETUP ee 5 As I ACKING BG Zenter ere 5 B Pore PRO WIG CO DEE 5 SCAN OI EE 5 IL USING YOUR HYBEX MICROARRAY INCUBATION SYSTEM ccsscccsssccssscccsscccsssccsscscssscessesneses 6 A Components Controls ANd ACcessorles eseseseesseesererssrrrssrersrrrssrresrerssrresreresrerssrresreesrersreresrers 6 B Handling the ale Da ge Cha al 7 C Optional Tube Blocks and Waterbath Insert cccccssscccssecccesceeescecaeeceeeeeceeeueceesescessuecesseceeenees ed D Using the Temperature Controller erreser 7 E Calibrating the EZ Zone Temperature Controller ccccccssecccsseccccesccceescecenececeeeeeeeessencesseeeeesens 8 IV MAINTAINING YOUR HYBEX SYSTEM csccsccscsscsscsscssssssesccsccessessessessccsecescessessassucsecescessussasencese 9 A Checking and Re2. existing offset value between the controller and digital thermometer is displayed Set Button Infinity Return Key Up Down Arrows Press and hold the SET key and use the up down arrows to adjust the offset value to the temperature difference calculated in Step 4 For example if the controller shows a temperature 1 5 C lower than the thermometer adjust the offset by adding 1 5 to the value shown Press the Infinity Key ee to exit calibration and return to the operation display Verify that the temperature on the thermometer matches the display Your incubator is now calibrated to provide accurate temperatures between 35 and 99 C Version 1 1E August 2012 12 408 733 7337 techserv scigene com SCIGEME mec microarray Incubation System USER MANUAL IX DECLARATION OF CONFORMITY Hybex Microarray and Microsample Incubation System SciGene 470 Lakeside Drive Suite F Sunnyvale CA 94085 4720 USA Declares that the above referenced product s meets the essential requirements of the following European Union Directives by using the relevant standards shown below to indicate compliance EMC Directive 89 336 EEC Standards EN 61326 1 1997 Electrical equipment for measurement control and laboratory use to include EN 61000 4 2 2001 A1 1998 A2 2001 A3 2003 EN 61000 4 3 2001 EN 61000 4 4 2001 EN 61000 4 6 2003 LVD Directive 2006 95 EC EN 61010 1 2004 Safety requirements for measurement control and laboratory u
3. temperature While holding down the SET button press the up or down arrow button until the desired set temperature is shown Release the SET button to display the actual temperature The instrument will adjust block heating until the SET temperature is attained B Calibrating the Watlow SD31 Temperature Controller Re calibrating the SD31 controller is recommended only if the display varies by more than one degree 1 C to a connected NIST certified digital thermometer SciGene cat 1051 52 0 To adjust the controller to achieve accurate temperatures L Insert a block into the Hybex incubator turn ON the instrument and set the controller to 65 C Allow 15 minutes to stabilize Using the cable provided with the digital thermometer plug one end into the blue receptacle found on the back panel of the Hybex incubator and the other end into the digital thermometer Turn on the thermometer Allow 1 minute for thermometer to stabilize The actual temperature of the block will be displayed Calculate the difference in the temperature shown on the controller and the digital thermometer For example if the controller displays 65 0 C and the digital thermometer displays 66 5 C the difference offset is 1 5 C On the temperature controller press the Infinity Key ee for Actual Temperature three seconds until OPEr appears Press the down arrow four times until Cal appears Press and hold the SET key The
4. Hybex Microarray Incubation System USER MANUAL Cat 1057 36 1 1057 36 2 FOR RESEARCH USE ONLY 470 Lakeside Dr Ste F Sunnyvale CA 94085 USA e Tel 408 733 7337 Fax 408 733 7336 techserv scigene com SCIGEME mec microarray Incubation System USER MANUAL Serial Number The following serial number identifies the specific instrument you have purchased and must be referenced when requesting service A copy is affixed to the instrument Technical Service 408 733 7337 techserv scigene com Warranty SciGene warrants that the heating unit described in this manual shall be free of defects in materials and workmanship for a period of 12 months from date of delivery This warranty does not cover removable blocks or accessories In the event of a defect during the warranty period SciGene s limit of liability will be to provide replacement parts at no charge or at its sole discretion replace the product The foregoing warranty is void in the event the unit was abused or modified or used in a manner inconsistent with its intended purpose SciGene makes no other warranty expressed or implied including warranties of merchantability and fitness for a particular purpose In no event shall SciGene be liable for any direct indirect special incidental or consequential damages or for any damages resulting from loss arising out of or in connection with the sale use or performance of the product Copyright Copyright 2004 2012
5. Repeat with second chamber Remove the handles from the chamber turn on power and set desired temperature C Optional Tube Blocks and Waterbath Insert Tube heating blocks and the waterbath insert are placed and removed from the heating unit with the aid of two bolts provided that act as handles To properly install a block or insert Thread the two bolts several turns into the block Grasp the ends of the bolts lift the block and then carefully lower it into the unit guiding it over the temperature probe The opening in the bottom of the block should fit over the probe 3 Remove the threaded bolts before using the system Do not operate the instrument without a block roperly inserted into the heating unit D Using the Temperature Controller Note For instruments manufactured prior to July 2012 instructions for using the SD 31 controller can be found in the Appendix of this document The Hybex Microarray Incubation System is equipped with an EZ Zone temperature controller The controller ae oa pill vient has two LED displays and four push buttons The left display shows ACTUAL block temperature in red The right display shows the SET or programmed temperature in green To enter the SET temperature simply push the up and Advance Button Up Down Arrows down arrows until the desired temperature is shown in Infinity Return Key the green display on the right The unit will adjust the heat of the block unti
6. e Hybex Microsample Incubator e Power cord e 2x Microarray incubation chambers e 2x Screw handle for chambers black e T handle Allen wrench for assembling chambers e Absorbent pads for inside chamber cover 25 pk e 2x Microarray slide racks shipped inside chamber e Handle for 4 position slide racks e Hybex Microarray Incubation System User Manual e Hybex Microarray Incubation Chamber User Guide C Installation Place the heating base on a level surface within a few feet of the power source The unit should be positioned to have clearance along the top so that the lid can be opened completely without interference Leave at least 3 inches of clearance from the back panel for air circulation Assemble and insert both incubation chambers into the heating base See Section III B for how to handle chambers Both incubation chambers a tube block or a waterbath insert must be placed inside the heating unit before turning on power Plug the provided power cord into the back of the unit and then to a properly grounded outlet Use only the power cord provided Turn on power to the system using the switch on the front of the unit Version 1 1E August 2012 5 408 733 7337 techserv scigene com SCIGEME mec microarray Incubation System USER MANUAL Ill USING YOUR HYBEX MICROARRAY INCUBATION SYSTEM A Components Controls and Accessories Note A heat block waterbath insert or microarray chambers are required for operat
7. he power switch to the OFF position and unplug the power cord before performing any service procedure There are two fuses located in a removable fuse block below the power cord receptacle on the back of the unit Digital Thermometer Jack E a ia Back of uni NS Use small screwdriver behind tab to open Fuse Block Fuse Block SE S Push clip up to release Fuse Holder To remove fuses 1 Insert a small flat blade screwdriver into the tab recess just below the plug receptacle Push down to release the fuse block Slide out the fuse holder from the fuse block while holding the retaining tab out of the way 4 Gently pry out the fuses A blown fuse appears dark Always replace fuses with those of the same amperage and voltage as shown on the label below the fuse block Version 1 1E August 2012 9 408 733 7337 techserv scigene com SCIGEME mec microarray Incubation System USER MANUAL G Cleaning d Any liquid or condensation in the block cavity of the instrument should be removed as soon as possible Clean the block cavity and temperature probe frequently to avoid build up of residue that can interfere with the heat control system The bottom of the cavity is sealed to provide temporary protection from spills and condensation Clean the metal surfaces and heated lid using a mild detergent based cleaner and wipe with a soft cloth Avoid abrasive cleaners
8. ion For a list of available accessories see section VII Ordering Information Name Puncgieon Fees t Tums on main powertounit For calibrating system temperature Optional accessories P Heated Lid Microarray Incubation Chamber Cat 1057 38 0 Kit 7 Temperature Probe BACK OF UNIT Heat Block 0 2 ml Cat 1057 31 0 0 6 ml Cat 1057 33 0 1 5 ml Cat 1057 34 0 Digital Power Cord Thermometer Receptacle Jack Waterbath Insert Cat 1057 35 0 Temperature Controller Power Switch Digital Thermometer Cat 1051 52 0 Heat Block for Flat Bottom Microplate Cat 1057 39 0 Version 1 1E August 2012 6 408 733 7337 techserv scigene com SCIGEME mec microarray Incubation System USER MANUAL B Handling the Incubation Chambers See the Hybex Microarray Incubation User Guide included with each chamber set for details on how to use the slide racks and assemble the chamber The following information is limited to how to insert and remove the chambers from the heating unit Chambers are inserted and removed with the aid of a single four pronged black plastic handle provided as follows Thread handle several turns into the chamber cover Lower the assembled chamber into the unit aligning the cutout on the side of the chamber with the temperature probe in the base 3
9. l the SET temperature is attained The controller is calibrated at the factory to provide accurate block temperatures from 35 to 99 C See section D Calibrating the Temperature Controller for details Version 1 1E August 2012 7 408 733 7337 techserv scigene com SCIGEME mec microarray Incubation System USER MANUAL E Calibrating the EZ Zone Temperature Controller Note For instruments manufactured prior to July 2012 instructions for calibrating the SD 31 controller can be found in the Appendix of this document The temperature controller comes calibrated from the factory to provide accurate block temperatures from 35 to 99 C The temperature controller will require calibration only if When checking the block temperature with a calibrated digital thermometer the thermometer temperature differs by more than one degree 1 C from the temperature shown on the controller display A NIST calibrated digital thermometer sold separately SciGene Cat 1051 52 0 is required to calibrate the unit Follow these steps to adjust the controller to achieve accurate temperatures Insert a block into the Hybex incubator turn ON the BACK OF UNIT instrument and set the controller to 65 C Allow 15 minutes for temperature to stabilize Using the cable provided with the digital thermometer plug one end into the blue receptacle found on the back panel of the Hybex incubator and the other end into the digital thermometer Tur
10. n on the thermometer Allow 1 minute for thermometer to stabilize The actual temperature of the block will be displayed Calculate the difference between the temperature shown on the EZ Zone controller and on the digital Digital Power Cord Thermometer Receptacle thermometer For example if the controller displays Jeck 65 0 C and the thermometer displays 66 5 C the difference offset is 1 5 C On the controller press the up and down arrows simultaneously for 3 seconds The left display shows A1 and the right display shows oPEr Press the Advance Button green circle 3 times until the right display shows i CA The left display will show the offset value between the controller and thermometer when the unit was last calibrated Use the up or down arrow to adjust the offset value to the temperature difference calculated in Step 4 For example if the controller shows a temperature 1 5 C lower than the thermometer adjust the offset by adding 1 5 to the value shown Press the Infinity Key ce twice to exit calibration and return to the operation display Verify that the temperature on the thermometer matches the display Your incubator is now calibrated to provide accurate temperatures between 35 and 99 C Version 1 1E August 2012 8 408 733 7337 techserv scigene com SCIGEME mec microarray Incubation System USER MANUAL IV MAINTAINING YOUR HYBEX SYSTEM F Checking and Replacing Fuses Turn t
11. placing ET 9 Be er TE 10 Vs TROUBLESHOOTING rssecce cereisc sa tcapasescecwnucciossacauseemnacnesisecseacceutesetcunessurceswsatetqstiensscesserecssmersesencses 11 Vi eeh gei KE 11 VIl ORDERING INFORMA TIO IN sossssrsrsnsnirs cents acecesaecen seme aaa 11 Vill APPENDIX A Using and Calibrating a Watlow SD31 Controller cscsccsccscsccsccsceccecesceees 12 A Using the Watlow SD31 Temperature Controller 12 B Calibrating the Watlow SD31 Temperature Controller cccccccssscccesececesececeeseeeeenceseeeceeseeeeens 12 IX DECLARATION OF CONFORMITY E 13 Version 1 1E August 2012 2 408 733 7337 techserv scigene com SCIGEME mec microarray Incubation System USER MANUAL A SAFETY NOTICES Intended Use The Hybex Microarray Incubation System is intended for the heating and incubation of laboratory samples The instrument should only be used according to the instructions provided in this manual If the equipment is used in a manner not specified by the manufacturer SciGene the protection provided by the equipment may be impaired Instrument Safety Before operating the system read the information in this section concerning hazards and potential hazards Ensure that anyone involved with the instrument s operation is instructed in both general safety practices for laboratories and specific safety practices for the instrument Symbols and Conventions The following chart is an illustrated glos
12. sary of the electrical symbols that are used on the Hybex Microsample Incubator heating base Whenever such symbols appear on instruments please observe appropriate safety measures Electrical Symbols This symbol indicates that this is a protected ground terminal that must be connected to earth ground before any other electrical connections are made to the instrument CAUTION This symbol alerts you to consult this Operator s Manual for further information and to proceed with caution This symbol indicates the ON position of the main POWER switch Non Electrical Symbols CAUTION This symbol illustrates a heat hazard Proceed with caution when working around these areas to avoid being burned by hot components CAUTION This symbol alerts you to consult this Operator s Manual for further information and to proceed with caution 1 C This symbol indicates the OFF position of the main POWER switch 2 Version 1 1E August 2012 3 408 733 7337 techserv scigene com SCIGEME mec microarray Incubation System USER MANUAL D Warnings Failure to comply with the following warnings that are affixed to the product can lead to possible personal injury or death This symbol on the rear of the instrument indicates the presence of the fuse box Warning For Continued Protection Against Fire Replace Only with Same Type Rating of Fuse Always disconnect the power cord before attempting to replace the fuse E Cautions Fail
13. se 2 Edition Part 1 General requirements Terry Gill Sunnyvale California USA Name of Authorized Representative Place of Issue Director of Product Manufacturing January 01 2012 Title of Authorized Representative Date of Issue ag Signature of Authorized Representative Version 1 1E August 2012 13 408 733 7337 techserv scigene com
14. that can scratch surfaces Version 1 1E August 2012 10 408 733 7337 techserv scigene com SCIGEME mec microarray Incubation System USER MANUAL V TROUBLESHOOTING Power switch light does not turn on Blown fuse s Replace fuse s on back of unit beneath power cord receptacle VI SPECIFICATIONS Cat 1057 36 1 115V AC 50 60 Hz 3A Cat 1057 36 2 230V AC 50 60 Hz 1 6A Outside cover closed H x W x D 10x6x12 inches 21x15x30 cm Instrument without block 6 Ibs 3 kg Performance and Controls Temperature Range Ambient 5 C to 99 C Temperature Controller Digital PID single loop Temperature Display Actual or Set single LED Digital Thermometer Output Thermocouple Vil ORDERING INFORMATION ewe Cpt um 1051 52 0 Digital thermometer Includes cable and NIST certificate Version 1 1E August 2012 11 408 733 7337 techserv scigene com SCIGEME mec microarray Incubation System USER MANUAL Vill APPENDIX A Using and Calibrating a Watlow SD31 Controller For selected Hybex incubators manufactured between 2005 and 2012 A Using the Watlow SD31 Temperature Controller The SD31 controller has a single LED panel with four buttons The set temperature is displayed if the SET key is pressed and held otherwise the ACTUAL block temperature is displayed To set the block temperature 1 2 3 Insert a block into the instrument and power on Hold in the SET button to view the previous set
15. ure to comply with the following cautionary statement affixed to the product may lead to possible personal injury A This symbol indicates the potential presence of a Hot Surface Use care when working in this area to avoid being burned F Compliance 1 European Community C s All instruments shipped to the European Union EU formerly known as the European Community have the CE label on the back of the instrument signifying that these instruments comply with the Electromagnetic Compatibility and Low Voltage Directives 2 US and Canadian Listings All instruments meet Laboratory Equipment standards UL 61010 1 2004 R7 05 and CAN CSA C22 2 61010 1 2004 Version 1 1E August 2012 4 408 733 7337 techserv scigene com SCIGEME mec microarray Incubation System USER MANUAL ll UNPACKING AND SET UP A Unpacking the System The Hybex Microsample Incubator heating base is shipped on its side within the interior carton Remove any packing material around the unit and lift the unit out taking care not to damage the hinged cover Microarray chambers tools and accessories are packaged separately from the heating base Carefully inspect the heating unit for damage If there is evidence of damage do not discard the shipping materials since they may be needed to return the unit Contact techserv scigene com for assistance B Parts Provided The following items are included with the Hybex Microarray Incubation System
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