OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR
Contents
1. On request can be implemented using 1 mm or 2 mm banana jacks or using SMC connectors Very Important EXT 02F headstages are always labeled 02 see Figure 3 and must not be exchanged with headstages from other npi electronic EXT amplifiers e g the modular EXT 10 2F or EXT 10C which are labeled EXT Also Important The shield of the BNC connector of the headstage is connected to driven shield and must not be connected to ground version 4 1 page 9 EXT 02F User Manual 3 Operation The EXT 02F can be operated either using the inputs of the front panel 16 Figure 1 or using a headstage Very Important You must not use inputs at the front panel and a headstage for one channel simultaneously If the BNC connectors are used the headstage must be disconnected Also Important you encounter noise when operating the EXT 02F try to place the amplifier as far away as possible from the power supply This will avoid magnetic coupling of the power supply to the amplifier Extracellular measurements are mostly done in slices or in vivo where distortions of the signal caused by other instruments and the animal itself are very common Additionally extracellular signals are very small and have to be amplified enormously The drawback 15 that noise 1s amplified as well Therefore each channel of the EXT 02F 15 equipped with differential input that minimizes noise pick up Differential means that the s2. 1 POWER ON switch Switch for turning the EXT amplifier ON upper position or OFF lower position 2 POWER SUPPLY connector Connector for an external power supply 12V to 18V AC min 1A Important If you encounter noise when operating the EXT 02F try to place the amplifier as far away as possible from the power supply This will avoid magnetic coupling of the power supply to the amplifier version 4 1 page 8 EXT 02F User Manual 3 GROUND connector Banana plug providing ground The plug is connected internally to the shields of the BNC connectors 4 AUX A connector BNC connector for a user defined signal Standard is not connected 5 DC OUT connector BNC connector providing the measured DC signal with a fixed amplification of 10 This signal is not filtered Use of this connector allows for instance measuring the signal AC signal is provided at the front panel while watching the offset simultaneously 6 NOTCH ON A switch Switch that enables upper position or disables lower position the NOTCH filter optional 2 5 Headstage Option heodstage electrode holder REF 1 1 il Tow AMPLIFIER HEADSTAGE E RFE Figure 3 headstage optional and electrode holder optional and of the 02 Headstage Elements BNC connector for the electrode holder measuring electrode REF SMC connector for the reference electrode GND Ground connector holding bar gt U
3. AC HIGH PASS filtered or DC coupled with variable gain Two LEDs indicate whether the amplifier is running out of its linear range and an analog balance monitor makes the control of OFFSETS easy A built in audio speaker monitors the output signal of one channel A A THRESHOLD function in NOISE mode can be used for masking the noise of the baseline A phone jack allows connection of an external speaker HEAD PHONES or further audio amplification e g computer sound card active speakers power amplifier etc 2 3 Description of the Front Panel Q O 2 P 19 9 4 OY 19 Figure 1 EXT 02F 1 front panel view one channel version version 4 1 page 4 EXT 02F User Manual In the following description of the front panel elements each element has a number that 1s related to that in Figure 1 The number is followed by the name in uppercase letters written on the front panel and the type of the element in lowercase letters Then a short description of the element is given Since the front panel elements for the two channel EXT 02F are identical for each channel A and B with identical functions and labels these elements are numbered and described only once for channel A 1 BALANCE x10mV meter wuer Analog BALANCE monitor that displays the OFFSET in the range of 30 mV and is used for optimal cancellation of the OFFSET 2 HIGHPASS Hz switch 12 position switch f
4. Electronic Instruments for the Life Sciences ade OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE EXT 02F EXTRACELLULAR AMPLIFIER WITH FILTERS One or Two Channel Recordings VERSION 4 1 2013 npi electronic GmbH Bauhofring 16 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 support npielectronic com http www npielectronic com EXT 02F User Manual Table of Contents ts T 3 De 0 0 MM M 4 2010 C OIDOHE i oe sette Iota 4 2 25 29 y stem cda idis iude cu Wyse iine ad 4 2 5 Descrpuoncor the Front Panel iio eoe i etui unio Gba sd tite etd 4 24 Description the 8 2 3 Headstage Option oases eaa E E a E 9 Headstape EIMON ee 9 J OpPera 10 12 SM c 13 version 4 1 page 2 EXT 02F User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic are NOT intended for clinical use or medical purposes e g for diagnosis or treatment of humans or for any other life supporting system
5. J Neurosci 16 2175 2185 _I Kettenmann amp Grantyn R eds 1992 Practical Electrophysiological Methods Wiley Liss New York 1 Kowski A B Veh W amp Weiss T 2009 Dopaminergic activation excites rat lateral habenular neurons in vivo Neuroscience 161 1154 1165 1 Lalley P M Moschovakis and U Windhorst 1999 Electrical Activity of Individual Neurons In Situ Extra and Intracellular Recording in U Windhorst and H Johansson eds Modern Techniques in Neuroscience Research Springer Berlin New York Lambert M Beck J C Baker R amp Straka H 2008 Semicircular canal size determines the developmental onset of angular vestibuloocular reflexes in larval Xenopus Journal of Neuroscience 28 8086 8095 1 Meuth S G Aller M I Munsch T Schuhmacher T Seidenbecher T Meuth P Kleinschnitz C Pape H C Wiendl H Wisden W amp Budde T 2006 The contribution of TASK 1 containing channels to the function of dorsal lateral geniculate thalamocortical relay neurons Molecular Pharmacology LJ M ller Ch M 1992 Extra and Intracellular Recording in the Slice in Kettenmann amp Grantyn R eds Practical Electro physiological Methods Wiley Liss New York L Ogden D ed 1992 Microelectrode Techniques The Plymouth Workshop Handbook Second Edition The Company of Biologists Ltd Cambridge L Schneider L amp Stengl M 2006 Ga
6. also ground the headstage inputs REF and if set to OFF 15 HEADSTAGE connector Connector for the optional headstage see also chapter 3 version 4 1 page 7 EXT 02F User Manual 16 IN connectors BNC connectors for connecting the INPUT signal if no headstage 1s used input 7 resistance 1 The inputs be used in single ended or differential configuration In differential configuration the signal which is connected to IN is subtracted from the signal that is connected to IN Usually the reference electrode is connected to IN connector If only one INPUT is used single ended configuration the other INPUT must be grounded using switch 14 see also 14 and chapter 3 Very Important You must not use inputs at the front panel and a headstage for one channel simultaneously If the BNC connectors are used the headstage must be disconnected Also very Important Setting both switches to OFF means that both inputs are grounded i e measurement is impossible This applies also if a headstage is used since these switches will also ground the headstage inputs REF and Perr if set to OFF 2 4 Description of the Rear Panel PLANER SUPPLY Figure 2 EXT 02F rear panel view Since the rear panel elements for the two channel EXT 02F are identical for each channel A and B with identical functions and labels these elements are numbered and described only once for channel A
7. as well as positive OFFSETS can be cancelled Audio Monitor 7 THRESHOLD potentiometer Potentiometer for setting the THRESHOLD in of the value set by INPUT MODE switch 8 0 5 V 1 V 2 V For example 1f the INPUT MODE switch 8 is set to 2 V and the THRESHOLD potentiometer 15 set to 50 the resulting THRESHOLD for monitoring the OUTPUT signal will be at 1 V 1 only signals greater than V will be converted into a sound NOISE With an amplification of 10000 this correspondents to a signal of 100 uV at the electrode THRESHOLD 8 INPUT MODE switch Switch for selecting the INPUT MODE PITCH The voltage of the INPUT signal 1s converted into a tone with a frequency equivalent to the amplitude of the INPUT voltage OFF The audio monitor 15 switched OFF NOISE voltage of INPUT signal is high pass filtered amplified and transduced to a sound 2V 1V 0 5 V The audio monitor works in NOISE MODE with THRESHOLD function 100 THRESHOLD 15 set to the selected value 2 V 1 V or 0 5 V see also 7 9 PHONES connector Stereo jack connector for PHONES an external amplifier e g active speakers PHONES 10 VOLUME potentiometer z Potentiometer for setting the VOLUME of the internal speaker or PHONES linked to connector 9 Turning clockwise will turn up the sound VOLUME For 2 2 two channel amplifier SOURCE switch Switch for selectin
8. g the channel to be monitored channel A or channel B 7 B SOURCE version 4 1 page 6 EXT 02F User Manual 11 GAIN switch 10 position switch for selecting the GAIN of the output signal at the OUTPUT connector 12 Amplification factors x10 x20 x50 x100 x200 x500 x1k X2k x5k x10k 12 OUTPUT connector BNC connector providing the recorded and conditioned signal 13 ZERO ADJ trimpot Trimpot for compensating for amplifier offsets If the baseline is not zero even 1040 if the input is grounded INPUT switches 14 set to OFF this offset can be compensated for Compensation procedure L Set the input switches 14 to OFF to ground the inputs and set the HIGH PASS FILTER to 400 Hz L Set the GAIN range switch to 1k and adjust the baseline to zero using the trimpot L Set the GAIN range switch to and adjust the baseline again if necessary 14 INPUT switches Switches for grounding the INPUT connectors 16 or the headstage connector 15 One switch for the IN and one for the IN Important In differential measurements both switches for IN and IN have to be set to ON In single ended measurements the switch for the INPUT that is not used must be set to OFF see also chapter 3 Also very Important Setting both switches to OFF means that both inputs are grounded 1 e measurement is impossible This applies also headstage is used since these switches will
9. hamber Slice Ag AgCl pellet Electrode holder BMC Jack EL REF ND SUBCLICK Ground connector jack REF Holding bar single ended measurement configuration Figure 4 headstage connections A differential measurement B single ended measurement Hint The REF connector of the headstage can also be set to GND by grounding the IN connector at the front panel using 14 Figure 1 version 4 1 page 11 EXT 02F User Manual 4 Literature I Barmashenko G Eysel U T amp Mittmann T 2003 Changes in intracellular calcium transients and LTP in the surround of visual cortex lesions in rats Brain Res 990 120 128 1 Boulton A A Baker B amp Vanderwolf C H eds 1990 Neurophysiological Techniques Basic Methods and Concepts Humana Press Clifton New Jersey L Huemmeke M Eysel U T amp Mittmann T 2002 Metabotropic glutamate receptors mediate expression of LTP in slices of rat visual cortex Eur J Neurosci 15 1641 1645 Ld Huemmeke M Eysel U T amp Mittmann 2004 Lesion induced enhancement of LTP in rat visual cortex is mediated by NMDA receptors containing the NR2B subunit J Physiol 559 875 882 1 Schulz D Huston J P Jezek K Haas L Roth Harer A Selbach O amp Luhmann H J 2002 Water maze performance exploratory activity inhibitory avoidance and hippocampal plasticity in aged superior and inferior learners Eur
10. ignal for the amplifier 15 the difference between the positive at the headstage and negative REF at the headstage input of the amplifier This results 1n canceling of all signals which both electrodes record e g noise For differential measurements both inputs are connected to electrodes using cables with grounded enclosure or electrode holders is connected to the measuring electrode and REF to the reference electrode If no headstage 15 used the switches 14 Figure have 10 be in the same position 1 e both set to ON The experimental chamber is grounded by an Ag AgCI pellet or an AGAR bridge connected to GND of the headstage see Figure 4 Important If differential measurement is not required single ended measurement configuration the REF input must be connected to ground GND at the headstage see Figure 4 or IN switch 14 Figure 1 must be set to OFF If headstage is used the switch corresponding to the unused INPUT has to be set to OFF see 14 Figure 1 The amplifier is in an undefined state if the REF is left open and can go into saturation making reliable measurements impossible version 4 1 page 10 EXT 02F User Manual Experimental chamber slice REF Ag AgCI electrode pellet Electrode holder BNC jack EL REF GND SUBCLICK Ground connector jack REF Holding bar differential rrecasurnerrent configuration Experimental c
11. npi electronic disclaims any warranties for such purpose Equipment supplied by npi electronic must be operated only by selected trained and adequately instructed personnel For details please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic D 71732 Tamm Germany 1 2 3 4 5 GENERAL This system is designed for use in scientific laboratories must operated by trained staff only General safety regulations for operating electrical devices should be followed AC MAINS CONNECTION While working with the npi systems always adhere to the appropriate safety measures for handling electronic devices Before using any device please read manuals and instructions carefully The device is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the instrument when replacing the fuse or changing line voltage Replace fuse only with an appropriate specified type STATIC ELECTRICITY Electronic equipment 15 sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic charge and must therefore be handled with care Electrostatic discharge can be avoided b
12. or selecting the corner frequency of the single pole HIGHPASS filter with 6 dB octave In OFF position the HIGHPASS filter is disabled DC recording Corner frequencies Hz 3 30 100 300 400 500 600 800 1k 3k 10k Note combination of both LOWPASS and HIGHPASS FILTER leads to a filter with bandpass characteristics 3 OVERLOAD LEDs LEDs that indicate if the amplifier works at 10 below it s positive or negative limit e The linear range of the amplifier is 12 V 4 LOWPASS Hz switch 12 position switch for selecting the corner frequency of the single pole LOWPASS filter with 6 dB octave Corner frequencies 30 100 300 500 700 900 1 3k 1 5k 1 7k 3k 10k 20k Hz Note A combination of both LOWPASS and HIGHPASS FILTER leads to a filter with bandpass characteristics 5 OFFSET range switch 2 Switch to set range for OFFSET potentiometer 6 1V corresponds to a range from 1000 mV to 1000 mV at the electrode 0 1 corresponds to a range from 100 mV to 100 mV at the electrode version 4 1 page 5 EXT 02F User Manual 6 OFFSET potentiometer OFFSET g Potentiometer for OFFSET compensation for the measured potential in DC mode M The RANGE is set by switch 5 The analog BALANCE monitor 1 displays the OFFSET in range of 30 mV and can be used for optimal cancellation of the OFFSET j Note The middle position corresponds to 0 V OFFSET since negative
13. p junctions between accessory medulla neurons appear to synchronize circadian clock cells of the cockroach Leucophaea maderae J Neurophysiol 95 1996 2002 I Seidenbecher T and H C Pape 2001 Contribution of intralaminar thalamic nuclei to spike and wave discharges during spontaneous seizures in a genetic rat model of absence epilepsy European Journal of Neuroscience Vol 13 1537 1546 L Windhorst U and H Johansson eds 1999 Modern Techniques in Neuroscience Research Springer Berlin Heidelberg New York version 4 1 page 12 5 Technical Data EXT 02F User Manual Input BNC connectors 10 range 1 V Input headstage Differential Input Input Capacitance OFFSET compensation HIGHPASS Filter LOWPASS Filter GAIN Overload LEDs Output Potential Monitor Power Supply Dimensions Headstage optional Input headstage Electrode connector Headstage Size Holding Bar version 4 1 gt 10 range 1 V CMR gt 90 dB at kHz tested with 0 input resistance 30 pF set by potentiometer range 40 1 1 V set by toggle switch single pole attenuation 6 dB octave corner frequencies Hz 3 30 100 300 400 500 600 800 Ik 3k with OFF position single pole attenuation 6 dB octave corner frequencies Hz 30 100 300 500 700 900 1 3k 1 5k 1 7k 3k 10k 20k rotary switch 10 20 50 100 200 500 1k 2k 5k 10k starting to light up at approx 10 V
14. range 10 V into 1 V into 50 Q analog display for the electrode offset range 30 mV 12V 18V AC min 1 A 245 x 200 x 90 mm gt 10 range 1 V BNC shield connected to driven shield 70 x 26 x 26 mm length 150 mm diameter 8 mm page 13
15. y touching a grounded metal surface when changing or adjusting sensors Always turn power off when adding or removing modules connecting or disconnecting sensors headstages or other components from the instrument or 19 cabinet TEMPERATURE DRIFT WARM UP TIME analog electronic systems are sensitive to temperature changes Therefore all electronic instruments containing analog circuits should be used only in a warmed up condition 1 e after internal temperature has reached steady state values In most cases warm up period of 20 30 minutes 15 sufficient HANDLING Please protect the device from moisture heat radiation and corrosive chemicals version 4 1 page 3 EXT 02F User Manual 2 EXT 02F 2 1 Components The following items are shipped with the EXT 02F system Amplifier EXT 02F 2 or EXT 02F 1 X User manual Optional Differential headstage s with connectors 2 2 System Description The 02 is designed for extracellular recordings of small voltage signals The standard model 02 2 comprises of two identical channels but a one channel version EXT 02F 1 is also available In the following description of the system only one channel is mentioned because front panel elements of the two recording channels are identical The 02 has a differential input with high input impedance to avoid noise The output voltage signal can be HIGH PASS or LOW PASS filtered and is available either
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