OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR
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1. WAIT GPCTR1_OUT Walt for Trigger signal only in PULSE mode LOW 0 V by default Gets HIGH 5 V if CellWorks waits for a trigger and remains high until STARTSCAN has been started see Figure 2 Note OUT is set by software and therefore not very precise a few ms version 3 6 page 7 INT 20X User Manual 12 ISYNC Synchronization signal LOW 0 V by default Gets STARTSCAN HIGH 5 V for 500 ns before each scan i e during data acquisition this signal has the same frequency as the sample rate set in CellWorks see Figure 2 Note STARTSCAN is set by hardware and therefore precise Table 1 front panel elements of the INT 20X breakout box The signals at the front panel can be divided into 5 groups Ai Ao Do TRIG IN TRIG OUT Each group is indicated at the front panel by a surrounding white line Analog input Ai0 Ai15 The 16 analog input lines from the PCI M Series board are linked to BNC connectors at the front panel These channels have a maximum voltage range of 10 V and cab be configured by software The maximum sample rate and the resolution is dependent on the PCI board that is installed A resolution of up to 16 bit and sample rate up to 1 25 MS s Mega Samples per second is possible We recommend to use sampling rates of 10 20 times the maximum measured frequency to avoid aliasing the sampling frequency must be 22 x fmax The shields2. for controlling valves and a npi amplifier contact npi for detailed information on additional hardware Important You must install and configure the computer board s first before you connect the other hardware components Please read the NI user manual of the appropriate board for instructions on installing and configuring the boards version 3 6 page 4 INT 20X User Manual 2 2 Parts Shipped with the Breakout Box Q Breakout box INT 20X Breakout box User Manual Optional accessories 68 pole SCSI cable External power supply 4 or 8 Channel Bath Perfusion System ALA Filter modules to avoid aliasing or to reduce noise Amplifiers CellWorks E Hard and Software from National Instruments L LI version 3 6 5 INT 20X User Manual 2 3 Description of the Front Panel Figure 1 shows the INT 20X front panel Table 1 includes a short description of all front panel elements The names brackets the signal denotations used in the PCI 5 User Manuals from National Instruments 018 40 000 e e n prb 6 4 Q oo 3 1 J Figure 1 front panel of INT 20X breakout box version 3 6 page 6 INT 20X User Manual Front panel Number Short name Signal name Description
3. 1 Ai0 Ail5 ACH0 ACH15 Analog input channels Analog Input 16 BNC connectors providing the 16 analog input channels of the B M or E series board AGND AISENSE Analog input ground Analog Input Sense This connector supplies the reference point for all analog input channels GND Ground Chassis potential 0 1 DAC0 DAC1 Analog output channels Analog Outputs These BNC connectors provide the 2 analog output channels of the B M or E series board DoO Do7 Digital output ports Digital I O These output ports provide the 8 digital lines from the E Series board GND Ground Compare to number 3 DGND DGND Digital ground Digital Ground This connector supplies the reference point for all digital signals TRIG Manual trigger This push button triggers the B M or E series board manually TRIG Trigger input This BNC connector provides the timing signal from the E Series board for connecting an external trigger device with active low logic 10 PC TRIG PWR PC connection Trigger Power supply connection LED PC indicates the connection between the computer and the breakout box computer must be switched on LED TRIG indicates the Trigger status green trigger active red waiting for trigger LED PWR indicates that an external power supply is plugged in 11
4. of all Ai BNC connectors are linked to the AISENSE signal and the AISENSE is connected to the AIGRND signal from the PCI Series board The INT 20X supports two modes of data acquisition NRSE Non Referenced Single Ended and DIFF Differential For further information please read the PCI Series user manual Usually NRSE is used Analog output A00 A01 The two analog outputs from the PCI board are available at the front panel Each channel has two BNC connectors With these ports it is possible to generate analog voltage signals in a range of 10V The resolution and the maximum update rate is dependent on the PCI board For further information please read the technical data of the PCI board that is connected Digital output Do0 Do7 The 8 digital I O Input Output lines of the PCI board are linked to the front panel The signals are fixed in output direction and buffered Driving several TTL inputs of external devices is possible version 3 6 page 8 INT 20X User Manual Control inputs and control outputs TRIG IN TRIG OUT The PCI board provides a lot of timing signals and PFI signals Programmable Function Input to control the PCI board by external devices or vice versa 2 input signals and 2 output signals are linked to BNC connectors at the front panel The following table shows the default configuration Note The OUT signal is linked to the BNC WAIT connector only in PULSE mode operation of CellWorks a
5. rear panel of the INT 20X breakout box version 3 6 page 10 INT 20X User Manual Rear panel Number Description 1 B or ESERIES BOARD 5 5168 pole male connector to connect the B M or E series board 2 POWER OUPUT SUBD 9 female connector to control directly valves of a perfusion system etc 3 PWR SUP Connector for the external power supply Table 2 rear panel elements of the INT 20X breakout box Three elements located at the rear panel of the INT 20X are necessary to interface the breakout box to the PCI board to external devices e g to a perfusion system and to connect an external power supply to the INT 20X PWR SUP The external power supply must be connected to the round connector PWR SUP Power supplies up to a maximum voltage of 36V can be plugged in Important Attention to the right polarity The core pine is positive and the coat is negative POWER OUTPUT Some applications need more power more current and higher voltage than a ordinary TTL output can supply The SUBD 9 female connector POWER OUTPUT supplies 8 digital power output channels The voltage value depends on the external power supply you have plugged in usually 12 V Thus direct valve switching is possible The digital port A B of the PCI board is linked and powered up to this output The pinout of this connector is shown in Table 3 and Table 4 Pins 1 to 8 are linked directly to the SUBD 9 female connect
6. Electronic Instruments t A for the Life Sciences ease wade OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE INT 20X BREAKOUT BOX FOR RECORDING AND GENERATING ELECTRICAL SIGNALS 200 XN NX BH RJ VERSION 3 6 npi 2014 npi electronic GmbH Bauhofring 16 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 support npielectronic com http www npielectronic com INT 20X User Manual Table of Contents T sSatet y Regulations oo ore deoa y kas 3 2 INT 20X Breakout Boka sas 4 2 1 System 4 2 2 Parts Shipped with the Breakout 5 2 3 Description of the Fr nt Panel uie iare ore aos 6 2 Description of the Rear Panel a i petens edet packed 10 3 Hardware Connections eei rid de ee Ai Lose ter pecu aet 14 4 Technical Data uen oath or etie aun anan sus 15 Inde X uote tanan l apama n mte e ur n 16 version 3 6 page 2 INT 20X User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic are NOT intended for clinical use or medical purposes e g for diagnosis or treatment of humans or for any other
7. GND 26 ACH13 60 27 AIGND 61 12 28 4 62 AISENSE 29 AIGND 63 ACHII 30 64 AIGND 31 ACH10 65 AIGND 32 AIGND 66 ACH9 33 ACHI 67 AIGND 34 ACHS 68 ACHO Table 5 pinout of the SCSI 68 pole male connector PCI M or B SERIES BOARD version 3 6 page 13 INT 20X User Manual 3 Hardware Connections Usually this universal breakout box is used in electrophysiological and pharmacological experiments Figure 4 shows how to connect the INT 20X breakout box to the computer with the boards and optionally a perfusion system with 8 valves Important It is necessary first to install and configure the NI data acquisition board s and then to connect the other hardware components Please read the NI user manual of the appropriate board information on installing and configuring the boards Computer with I O Boards and Ports M e m amp 2 ae Li PCI E Series Board e g 6024E SCSI 68 pole dongle cable ooo Power Supply INT 20X Rear Panel electronic GmbH TYPE INT 20X SERIAL N 0030092 D 71732 Tamm Germany DATE 06 18 2003 www nplelectronic com i 5 9 pole male male cable Perfusion System Figure 4 sample hardware connection of the INT 20X version 3 6 page 14 INT 20X User Manual 4 Technical Data Analog Input Number of cha
8. atic discharge can be avoided by touching a grounded metal surface when changing or adjusting sensors Always turn power off when adding or removing modules connecting or disconnecting sensors headstages or other components from the instrument or 19 cabinet TEMPERATURE DRIFT WARM UP TIME All analog electronic systems are sensitive to temperature changes Therefore all electronic instruments containing analog circuits should be used only in a warmed up condition i e after internal temperature has reached steady state values In most cases a warm up period of 20 30 minutes is sufficient HANDLING Please protect the device from moisture heat radiation and corrosive chemicals I O BOARDS This breakout box can be used only with computer boards from National Instruments These I O boards must be installed and configured first For the correct installation and configuration read the user manual of the appropriate board shipped with the board VERY IMPORTANT Always turn power off when connecting or disconnecting components at the rear panel of the breakout box to avoid any damage version 3 6 page 3 INT 20X User Manual 2 INT 20X Breakout Box 2 1 System Description The INT 20X is a universal breakout box providing access to the PCI B series and PCI M series multifunction I O boards from National Instruments with a 68 pole SCSI connector male like 6221 low cost 16 bit M series board PCI 6014 low
9. cost 16 bit B series board Windows only The standard system consists of the INT 20X breakout box B M or E series board and software to record up to 16 analog signals and store the data on hard disk 2 analog output channels to generate analog signals and a digital port with 8 digital lines to write digital signals TTL are available as well Usually this breakout box is used with the software package CellWorks E but it is also possible to write own programs for example with the program development application LabVIEW from National Instruments CellWorks is a modular program especially developed for electrophysiological and pharmacological experiments It is used to automate experimental protocols under computer control Contact npi for more information on CellWorks The free WinWCP and Win EDR software from the University of Strathclyde works also well with these NI boards and the INT 20X A digital POWER OUTPUT port with 8 lines e g to control the magnetic valves of perfusion systems is also available The INT 20X also provides a TRIG IN connector for triggering CellWorks from an external device and two TRIG OUT signals WAIT and SYNC WAIT indicates that the software is waiting for a trigger only in PULSE mode and SYNC can be used to synchronize external devices with the data acquisition rate Note The INT 20X breakout box does not include the I O connector for the PCI 6503 board If you intend to use more than 8 digital lines e g
10. life supporting system npi electronic disclaims any warranties for such purpose Equipment supplied by npi electronic must be operated only by selected trained and adequately instructed personnel For details please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic D 71732 Tamm Germany 1 2 3 4 5 7 GENERAL This system is designed for use in scientific laboratories and must be operated only by trained staff General safety regulations for operating electrical devices should be followed AC MAINS CONNECTION While working with the npi systems always adhere to the appropriate safety measures for handling electronic devices Before using any device please read manuals and instructions carefully The device is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the instrument when replacing the fuse or changing line voltage Replace fuse only with an appropriate specified type STATIC ELECTRICITY Electronic equipment is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic charge and must therefore be handled with care Electrost
11. nd therefore the trigger status LED is in CHART mode operation always green Signal from PCI board to Front panel BNC PFIO TRIGI Input BNC TRIG PFIO TRIGI Input push button TRIG GPCTR1_OUT only in PULSE mode Processed and linked to output BNC WAIT STARTSCAN Output BNC SYNC Front panel LEDs PC TRIG PWR There are three LEDs PC TRIG and PWR which are used to indicate proper connections to the computer or to external devices and the trigger status If the LED PC is on red the PCI E Series Board in the computer is connected properly to the breakout box the computer must be switched on The LED in the middle TRIG indicates the trigger status green trigger active red waiting for trigger also indicated in CellWorks by the red field in the Execution Module If the LED PWR is red an external power supply is properly connected Start Start Data Acquisition Data Acquisition Begin of Begin of for Trigger for Trigger 5 V WAIT OV 5V SYNC OV Figure 2 time course of trigger out signals version 3 6 page 9 INT 20X User Manual 2 4 Description of the Rear Panel Figure 3 shows the INT 20X rear panel Table 2 gives a short description of the INT 20X rear panel elements nplelectronic GmbH TYPE INT 20X SERIAL N 0030092 DATE 06 18 2003 D 71732 Tamm Germany www npielectronic com 37 3 d Figure 3
12. nnels in NRSE Mode 16 DIFF Mode 8 Input resistance IMO Max Input range bipolar 10V unipolar 0 10V Input coupling DC Transfer characteristics depend on the board type which is used Please read the appropriate user manual from National Instruments shipped with the board as PDF document Analog Output Number of channels 2 Voltage range 10V Output coupling DC Transfer characteristics depend on the board type which is used Please read the appropriate user manual from National Instruments shipped with the board as PDF document Digital Number of channels 8 Compatibility TTL CMOS Power Input External Power Input for valve control 12 36 V Power Output Maximum Voltage Input voltage 0 5V Maximum Current per channel only one channel active 500mA version 3 6 page 15 INT 20X User Manual Index analog input 8 analog output 8 CellWorks 4 connector PCI M series board 13 Data acquisition board 4 digital output 8 front panel 6 front panel elements 8 Optional accessories 5 perfusion system 14 power output 11 power supply 11 rear panel elements 11 SUBD 9 connector 12 technical data 15 trigger 9 version 3 6 page 16
13. or of the BPS system and are going to the respective valves Pin 9 is linked to Pin 9 of the SUBD 9 female connector of the BPS system and provides power usually 12V All are switched open collector Note This power port is configured in active low logic A step from low to high from 0V to 5 Volt causes step from V power supply to OV version 3 6 page 11 INT 20X User Manual 1 2 3 4 5 6 7 8 9 Table 3 pinout of the SUBD 9 female connector POWER OUTPUT 9 pol SUBD Pin numberino Table 4 numbering of SUBD 9 connectors female version 3 6 page 12 INT 20X User Manual PCI B or M SERIES BOARD not PCI 6071E The PCI board is connected to the SCSI 68 pole male connector PCI M SERIES BOARD A 68 pole SCSI pin to pin cable is required Pin Name Pin Name 1 FFREQ OUT 35 DGND 2 OUT 36 DGND 3 PFI9 GPCTRO_GATE 37 PFI8 GPCTRO_SOURCE 4 DGND 38 PFI7T STARTSCAN 5 PFIG WFTRIG 39 DGND 6 PFIS5 UPDATE 40 GPCTRI OUT 7 DGND 41 PFIA GPCTRI 8 45V 42 PFI3 GPCTRI SOURCE 9 DGND 43 PFI2 CONVERT 10 PFII TRIG2 44 DGND 11 PFIO TRIG1 45 EXTSTROBE 12 DGND 46 SCANCLK 13 DGND 47 14 45V 48 DIO7 15 DGND 49 DIO2 16 DIO6 50 DGND 17 DIOI 51 DIOS5 18 DGND 52 DIOO 19 DIO4 53 DGND 20 EXTREF 54 AOGND 21 DACIOUT 55 AOGND 22 DACOOUT 56 AIGND 23 15 57 ACH7 24 AIGND 58 ACH14 25 ACH6 59 AI
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