Polaroid DCM 2 Online User's Manual
Contents
1. size and time required to transfer the final image from the camera to your computer See Image File Sizes 30 Image File Sizes The size of the saved TIFF image depends on image type and size as shown in the fol lowing table File sizes are approximate Image Size Pixels 48 Bit Color 24 Bit Color 16 Bit Grayscale 8 Bit Grayscale 1600 x 1200 11 300 kbyte 5 650 kbyte 3 800 kbyte 1 900 kbyte 800 x 600 2 900 kbyte 1 400 kbyte 1 000 kbyte 500 kbyte 400 x 300 700 kbyte 350 kbyte 240 kbyte 120 kbyte Windows only 31 Verifying Sharp Focus Digitally If necessary you can fine tune your image focus digitally with the DMC 2 software 1 2 3 4 Click the image to display the zoom and focus box Drag the zoom and focus box to the area of interest Zoom into the area of interest by clicking A Click Focus Tool on the Tools menu The Focus Tool box appears Adjust the microscope focus knob until the two colored indicator bars are farthest to the right The red bar indicates the maximum sharpness achieved so far The other bar indi cates the current sharpness Close the Focus Tool box by clicking the Close button in the upper right corner 32 Capturing the Image After you have selected the capture settings in the DMC 2 main window you are ready to capture the image and transfer it to the computer You have the following three options for capturing the image Capture the imag2. Polaroid Italia S p A Via Piave 11 21051 Arcisate Varese Tel 0269633253 Japan Nippon Polaroid Kabushiki Kaisha Mori Building No 30 2 2 Toranomon 3 chome Minato ku Tokyo 105 Tel 81 3 3438 8811 Fax 81 3 3433 3537 127 Nederland Polaroid Nederland B V Zonnebaan 45 3606 CH Maarssen P O Box 1219 3600 BE Maarssen Tel 0204874534 New Zealand Polaroid New Zealand Ltd Camera Services 24 26 Anzac Avenue Auckland Tel 09 377 3773 Next Luxembourg Tel 08002542 M xico Polaroid de M xico S A de C V Paseo de la Reforma 195 Piso 17 C P 06500 M xico DE Tel 703 1111 Fax 566 0505 Del interior al 91 800 70 727 Ventas 91 800 70 747 128 Norge Polaroid Norge A S Industriveien 8B 1473 Skarer Postboks 80 1471 Skarer Tlf 80011887 Osterreich Polaroid Ges m b H Eitnergasse 5A 1231 Wein Tel 06607433 Next Schweiz Suisse Svizzera Polaroid AG Hardturmstrasse 133 8037 Ziirich Tel 018385329 Deutsch Tel 018385348 Francais Tel 018385349 Italiano Suomi Polaroid Oy Sinikalliontie 10 02630 Esposo Puh 0800117008 129 Portugal Polaroid Espafia S A Sucursal em Portugal Edificio Monumental Avenue Praia da Vitoria 71 A 4 B 1050 Lisboa Tel 0505446017 Puerto Rico Polaroid Caribbean Corporation Centro de Seguros Ave Ponce de Le n 701 Miramar Santurce 00907 Tel 809 725 6240
3. camera body cleaning 98 camera optics cleaning 90 camera preferences 85 camera attaching to microscope 108 camera connecting to computer 110 Next 141 Index Continued camera DMC selecting 15 camera selecting 14 camera turning on 13 capture area choosing 17 capturing the image 33 Cast found on auto color balance 73 color balance adjusting automatically 44 color balance adjusting by selecting gray point 46 color balance adjusting by selecting whitepoint 45 color balance adjusting manually 48 color balance adusting by selecting blackpoint 47 Color Channel Mixture 80 Color Output Scale 81 color preview choosing 19 color stacker utility using 86 color temperature custom creating 27 Next 142 Index Continued color temperature setting 26 communication problems SCSI solving 133 copyright notice 124 D dark levels adjusting manually 49 dark subtract using to reduce grain 56 device not recognized solving 134 DMC Ie cameras software compatibility 10 Do auto color balance 72 E EMI notice Canada 122 EMI notice Europe 123 enhancing the capture image 43 enlarging the preview 21 Next 143 Index Continued exposure automatic calibrating 23 exposure setting automatically 22 exposure setting manually 24 F FCC notice 120 features DMC 2 camera 9 file sizes based on type and image sizes 31 flash using 74 fluorochromes combining with Col
4. Fax 809 725 5462 Next Sverige Polaroid AB Ekholmsvagen 36 Box 204 127 27 Sk rholmen Tel 084451231 U K Polaroid U K Ltd amp Polaroid Export Operations Wheathampstead House Codicote Road Wheathampstead Hertfordshire AL4 8SF Tel 0181 308 6508 U K 44 181 308 6593 Export 130 U S A Polaroid Corporation Technical Support Toll free 1 800 432 5355 131 Technical Support If you need additional help with your Polaroid equipment call your nearest Polaroid office at the number listed in Polaroid Offices and Service Centers Polaroid offers a variety of customer support services Call for details and applicable fees You may also write to Digital Imaging Technical Support 201 Burlington Road 1 2A Bedford MA 01730 Internet Support Technical support is also available over the Internet Connect to the Polaroid Web site at www polaroid com Application Support If you need help with your application software please contact the manufacturer of the software 132 Solving SCSI Communication Problems SCSI device not recognized Horizontal lines sections missing from image System stops responding during image transfer 133 Problem Device not recognized Failure of your computer to recognize a SCSI device could be caused by any of the fol lowing Device not turned on Turn the power switch on See instructions for turning the device on C
5. Fluorescent Optimizes the output color for fluorescent microscopy soft ware programs Next 80 Output Preferences Continued Color Channel Mixture continued User Use the custom color channel mixture in MATRIX2PUSER MTX for DMC 2 cameras or MATRIXIEUSER MTX for DMC Ie cameras Color Output Scale Tone Scale Optimizes the output image tone values for display on a monitor Linear Sets the tone value of each output pixel in proportion to the actual transmittance or reflectance measured by the camera Logarithmic Sets the tone value of each output pixel in proportion to the logarithm of the actual transmittance or reflectance measured by the camera Next 81 Output Preferences Continued Grayscale Channel Mixture The Grayscale Channel Mixture uses the following RGB channels to produce a grayscale image Luminance Digitally provides the effect of a luminance filter for gray scale imaging Red Provides the effect of a red filter Green Provides the effect of a green filter Blue Provides the effect of a blue filter Next 82 Output Preferences Continued Grayscale Channel Mixture continued Cyan DMC Ie cameras only Provides the effect of a cyan filter Magenta DMC Ie cameras only Provides the effect of a magenta filter Yellow DMC Ie cameras only Provides the effect of a yellow filter Next 83 Output Preferences Continued Grayscale Output Scale Tone Sca
6. Saving and Reusing Program Settings continued To load enhancement settings you saved earlier Macintosh Windows 1 In the Enhancement window click 1 In the main DMC 2 window click Settings Settings on the File menu to display 2 Select the name of the settings to be the Settings window applied 2 Click the name of the settings you want to load 3 Click OK Next 66 Saving and Reusing Program Settings continued To delete a settings file Macintosh Windows 1 In the Enhancement window click 1 In the main DMC 2 window click Settings Settings on the File menu to display 2 Select the name of the settings to be the Settings window applied 2 Click the name of the settings you 3 Click Delete want to delete 3 Click Delete 4 Click OK 67 Program Settings You Can Save When you save DMC 2 program settings for reuse see Saving and Reusing Program Settings the following settings are saved Main DMC Window Settings Color checkbox state Color Temperature e Exposure Image Type e Sensitivity e Image Size Enhance Window Settings Red e Brightness Green Gamma e Blue e Sharpness e Dark Levels Next 68 Program Settings You Can Save Continued Preferences Box General Settings On each image capture e Overwrite images during Rapid Fire Cast found on color balance Number of Light Frames e Show preview on startup e Number of Dark Frames e Use flash e L
7. continued compliance any changes or modifications not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment Product The Polaroid DMC 2 Microscope Camera Marketed by Polaroid Corporation Cambridge MA 02139 U S A Telephone 1 800 432 5355 Next 121 FCC Notice continued This device complies with Part 15 of the FCC Rules Operation is subject to the follow ing two considerations 1 This device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation EMI Notice Canada This equipment is a Class A digital apparatus which complies with the Radio Interfer ence Regulations C108 8 M1983 Cet quipment est un appareil num rique de la classe A qui se conforme aux R gle ments sur la brouillage radio lectrique C108 8 M1983 Next 122 Notice Europe Warning This is a Class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate mea sures Polaroid Corporation certifies that this product conforms to the following standards Low Voltage Directive 73 23 EEC EN60950 IEC 950 EMC directive 89 336 EEC EN 55022 EN 50082 1 IEC 1000 4 2 EN 61000 4 2 IEC 1000 4 3 EN 61000 4 3 IEC 1000 4 4 EN 61000 4 4 123 Copyright and Trademark Notices Cop
8. dimensions of features in the image To add a micron marker to the image 1 Ifyou have not already done so create a calibrated micron marker for the micro scope objective you are using Creating a calibrated micron marker Click the Add Micron Marker box Choose the marker appropriate for the microscope objective from the box that appears next to the Add Micron Marker box 58 Creating a Calibrated Micron Marker To create a calibrated micron marker you can add to the bottom of images for deter mining dimensions of features in the image Macintosh Windows 1 Place a micrometer horizontally 1 Select Calibrate Objectives from the under the microscope objective and Tools menu focus the microscope to obtain a sharp image of the scale in the DMC 2 main window 2 Click the Add Micron Marker box The Calibrate Objectives Tips box opens 2 Place a micrometer horizontally under the microscope objective and 3 Click the popup menu next to Add focus the microscope to obtain a Micron Marker box and select Cali sharp image of the scale in the DMC 2 brate Objective main window Next 59 Creating a Calibrated Micron Marker continued Macintosh Windows The Micron Marker Calibration win dow opens and displays the microme ter image 4 Define a measured distance on the micrometer image by dragging a line from one micrometer scale mark to another The measured distance appears as a gre
9. view a color preview image select Color in the main DMC 2 window To view a grayscale preview image deselect Color 19 Rotating the Image To correct picture orientation you can rotate the preview image 180 degrees by clicking the rotate button When you rotate the preview image the final image is also rotated 20 Enlarging the Preview You can enlarge the view of the preview image for focusing and closely inspecting detail while framing Enlarging the view does not affect the final image To enlarge the preview image 1 Click the image to display the zoom and focus box 2 Drag the zoom and focus box to the area of interest 3 To zoom in click EI To zoom out click al 21 Setting Exposure Automatically 1 Ifyou have not done so calibrate automatic exposure for the current lighting and specimen conditions See Calibrating Auto Exposure 2 Click the Auto button in the main DMC 2 window 3 Wait a few seconds for the DMC 2 software to optimize the exposure 22 Calibrating Auto Exposure 1 Manually set exposure to the optimum position for the current setup See Setting Exposure Manually Click Calibrate Auto Exposure on the Tools menu Click the Set button in the Auto Exposure Calibration box 23 Setting Exposure Manually Drag the Exposure slider right or left to adjust the camera shutter speed for brighten ing or darkening the image Range 20 millis
10. Apple System Profiles usually on Look at the SCSI address switch on each the Apple menu If it is not it may be SCSI device connected to your system If available from www apple com necessary see the instructions provided swupdates with the devices 2 Click Devices and Volumes to view the SCSI device addresses Next 111 Setting the Camera SCSI Address Continued Turn the camera power switch to off 0 and set the SCSI address switch to a value not used by any other device SCSI address switch Do not use 7 8or9 not valid addresses Power switch off 0 Note The address is preset to 4 Do not change it unless another device is using 4 Other addresses typically available are 2 5 and 6 Next 112 Connecting the SCSI Cables Place the DMC 2 camera at the end of the SCSI chain as shown or between the com puter and other SCSI devices Use the shortest possible cables Note Turn the computer camera and all other SCSI devices off before connecting or disconnecting any cables Do not connect the camera to the parallel connector on your computer Doing so could damage the camera or computer DMC camera termination on 1 ae f i i Computer U Other SCSI device not terminated Next 113 Setting Termination The last device in a SCSI chain must be terminated or have termination on while all other devices must be unterminated or hav
11. CD ROM Click OK Next 107 5 Follow instructions on the screen 6 Remove the CD from the drive Attaching the Camera to the Microscope You need a 0 63 or 1 1 c mount adapter not provided compatible with your micro scope Do not use a 0 5 c mount adapter Refer to your microscope manual for more information about c mounts Caution Verify that the threaded end of the c mount adapter does not exceed 0 25 in 6 3mm Longer adapters can damage the camera 0 25 in 6 3mm maximum Next 108 To attach the camera to the microscope 1 Screw the c mount adapter into the camera Note Finger tighten the adapter Do not use tools 2 Insert the c mount adapter into the microscope The camera indicator LEDs should face the front of the microscope Indicator LEDs Next 109 Connecting the Camera to the Computer The DMC 2 camera requires a SCSI host adapter in your computer If your computer does not have an adapter you must install one see Installing a SCSI Host Adapter If your computer is a PC compatible with a SCSI adapter but the adapter is connected to a hard drive installing a second adapter is recommended Next 110 Setting the Camera SCSI Address Each device connected to a SCSI host adapter must have a unique address from 0 6 To assure a unique address for the camera determine the addresses of any other SCSI devices Macintosh Windows 1 Run
12. Color Temperature Setting continued Macintosh Windows 4 Click Calibrate 5 Wait for the Calibrate Color Tempera ture box to close 6 To use the custom setting select Cus tom from the Color Temp popup menu 4 Click Begin 5 Wait for the Calibrate Custom Color Temperature box to close 6 To use the custom setting select Cus tom from the Color Temperature box 28 Selecting Image Type You can select one of these image types for your final image from the Image Type box e 48 Bit Color 68 7 billion colors e 24 Bit Color 16 7 million colors e 16 Bit Grayscale 65 000 gray shades e 8 Bit Grayscale 256 gray shades The image type you choose affects the size of the image file See Image File Sizes Note Before saving images as 48 bit color or 16 bit grayscale verify that other appli cations you intend to use support TIFF images in those formats For example Adobe Photoshop version 5 supports 48 bit color images but earlier versions do not 29 Selecting Image Size Choose a final image size from the Image Size box 1600 x 1200 pixels 800 x 600 pixels 400 x 300 pixels Windows systems only These sizes are available if your selected region of interest includes the entire frame If your region of interest is smaller the available image sizes correspond to the size of the selected region Larger image sizes usually improve image quality but they also increase the image file
13. DMC 2 Camera Specifications Continued Dimensions 2 1x7 2x5 1 in 5 3x18 3x13 0cm Power supply 100 250V 0 38A 50 60Hz surge protected input 6 VDC 2 0A output Safety approvals SA CE DVE Limited warranty 1 year parts and labor Note Specifications subject to change without notice 101 System Requirements Using the DMC 2 camera and software to preview capture and enhance pictures requires a minimum level of computer hardware and software If your system does not meet these requirements contact your Polaroid dealer or your computer dealer PC system requirements Macintosh system requirements 102 PC Minimum System Requirements 166 MHz Pentium processor and 64 megabytes MB of RAM 96MB of RAM for a TWAIN compliant image processing program such as Adobe Photoshop or Image Pro e Microsoft Windows 95 98 Me 2000 or NT 4 0 or later operating system e ASPI compliant SCSI adapter and the appropriate Windows drivers 24 bit color display adapter e Light microscope with a 63x 1 1 or larger C mount adapter 103 Macintosh Minimum System Requirements PowerPC Macintosh computer or compatible with a SCSI 2 interface and 64 mega bytes MB of RAM 96MB of RAM for a Photoshop Plug In compliant image pro cessing program such as Adobe Photoshop or Image Pro e Apple system software version 8 6 or later e QuickTime software version 4 0 3 or later e 80MB of availa
14. PDF and its associated Acrobat Reader software You should familiarize yourself with the controls of the Acrobat reader Access Help Reader Online Guide Viewing PDF Documents Navigating Pages for more information Polaroid electronic documents provide additional navigation tools at the top of the screen The icons have the following meanings Click to go to the table of contents Click to return to the previous page Next 7 Click to display these help pages Click T to open the index DMC 2 Camera Features The Polaroid DMC 2 Digital Microscope Camera captures high quality digital color images from light microscopes and other optical instruments The camera uses a 2 million pixel 11mm CCD charge coupled device to capture 14 bit linear RGB images It transfers these images in seconds to an IBM PC or Power Macintosh com patible computer in TIFF format with 1600x1200 or 800x600 pixels On IBM PC com patible systems an additional image size of 400x300 is available PT w Next 9 Using the software provided with the camera you can e Display a color viewfinder image on your computer screen Choose resolution exposure sensitivity color temperature and other options Automatically adjust exposure Manually adjust color balance brightness contrast and sharpness Verify focus digitally Add a calibrated scale to the image e Add a title or other text to the im
15. Polaroid Contents Navigating in This Document DMC 2 Camera Features Taking Pictures Typical Picture Taking Sequence Turning the Camera On Starting the DMC 2 Software Selecting a DMC Camera Framing and Focusing the Preview Choosing a Capture Area Choosing Color or Grayscale piin Rotating the Image Enlarging the Preview Setting Exposure Autamati ally Next e 7 e 9 12 D a JS 14 15 16 17 x ES 20 TA dl Calibrating Auto Exposure Setting Exposure Manually Setting Sensitivity Selecting Color Temperature Creating a Custom Color Temperature setting Selecting Image Type Selecting Image Size Image File Sizes Verifying Sharp Focus Digitally Capturing the Image Capturing a Rapid Sequence Printing the Image Enhancing the Image with DMC 2 Software Automatically Adjusting Color Balance Adjusting Color Balance by Selecting a Wihitepaint Adjusting Color Balance by Selecting a Neutral Gray Point Next Adjusting Color Balance by Selecting a Blackpoint Manually Adjusting Color Balance Manually Adjusting Dark Levels Manually Adjusting Brightness Adjusting Gamma Viewing a Histogram Adjusting Sharpness Viewing the Full Image Reducing Grain in Long Exposures Adding a Label i Adding a Calibrated Micron Mates Creating a Calibrated Micron Marker Deleting a Calibrated Micron Marker Saving a
16. Windows 2 Open the Color Stacker utility dou ble click Polaroid Color Stacker 3 Select an image for each color channel by clicking each color button Red Green and Blue and selecting the appropriate TIFF file 4 Click Create Composite select a folder and file name and save the composite image 2 Open the Color Stacker utility click the Windows Start button point to Polaroid Digital Microscope Camera 2 on the Programs menu and click Polaroid Color Stacker 3 Select an image for each color channel by clicking each color button Red Green and Blue and selecting the appropriate TIFF file 4 Click Create Composite select a folder and file name and save the composite image 87 Color Stacker Preview Options The Color Stacker utility displays a preview of the composite image as you select files for each color channel By clicking the Options button at the top of the Color Stacker window you can select from one of these composite preview options e Show Original Image e Show Relevant Channel e Add Channel Progressively 88 Camera Maintenance DMC 2 camera maintenance consists of cleaning the camera when necessary Click on either topic below for more information Cleaning the camera optics Cleaning the camera body 89 Cleaning the Camera Optics Important Protect the DMC 2 camera from dirt dust rain and moisture Damage caused by these conditions may not be cov
17. ables loose or disconnected Check all cable connections See instructions for connecting the SCSI cables Device turned on after computer Make sure the device is turned on then restart the computer Incorrect SCSI address Make sure the device has a unique SCSI address See instructions for setting the SCSI address of the device Next 134 Device not recognized continued Incorrect termination Check for proper SCSI chain termination See instructions for setting termination Outdated miniport and ASPI layer drivers Windows systems only Update the SCSI host adapter Windows miniport driver MPD files for your SCSI host adapter according to instructions provided with the adapter For Windows NT miniport drivers have the extension SYS Also make sure your system has the latest ASPI layer files in the WINDOWS SYSTEMS folder Updated drivers are often available from your adapter manufacturer s site on the World Wide Web DOS drivers in CONFIG SYS Windows systems only Edit the CONFIG SYS file to disable any SCSI drivers Add REM to beginning of any SCSI driver lines Incompatible SCSI host adapter Install an additional or different SCSI host adapter The adapter must be ASPI compliant 135 Symptom Horizontal lines sections missing from image If your images display horizontal lines or bands check the following Incorrect termination Check for proper SCSI chain termination See instruct
18. age Transfer the image to your image editing application or hard drive e Capture a rapid sequence of images and select one for transfer to your computer Note The DMC 2 software is compatible with DMC 2 and DMC Ie cameras Next 10 The camera has a SCSI connection requiring no frame grabber or other special hard ware or software The camera requires a SCSI host adapter usually built into Macin tosh computers SCSI host adapters can also be easily added to PC compatible computers Compatible with standard 63 1 1 or larger c mount adapters the camera attaches easily to microscopes for most types of transmitted light and reflected light micros copy Multiple DMC 2 cameras can be connected in a SCSI chain to a single computer simul taneously for capturing images from multiple instruments without moving the cam era or reconnecting cables The camera also features a tripod mount x sync connection and remote shutter con nection for use with standard c mount lenses on a copystand or tripod Detailed camera specifications 11 Taking Pictures Typical Picture Taking Sequence 1 Turn on the camera 9 Set sensitivity 2 Start the DMC 2 software 10 Select color temperature 3 Frame and focus the preview 11 Select image type 4 Choose a capture area 12 Select image size 5 Choose color or grayscale preview 13 Verify sharp focus digitally 6 Rotate the image 14 Capture the image 7 Zoom the prev
19. ame of the program file to which you want to transfer the DMC images You can also click the Browse but ton and select the program file Next 38 5 Click OK When you click Capture amp Save or the save button in the DMC 2 program the image appears in the selected application for further enhancement and saving If you are running the TWAIN version of DMC 2 software If you are in the DMC 2 main window click Capture amp Transfer Wait for the image to appear in your program If you are in the DMC 2 Enhance window or the Full Image window click the transfer button Wait for the image to appear in your program 8 pp your prog After the image appears in your program you can edit print or save it according to instructions provided with the program 39 Capturing a Rapid Sequence The DMC camera and software allows you to capture a rapid sequence of images in the camera memory After the images are stored you can select one of the captured images for transfer to the DMC 2 Enhance window or for saving immediately To capture a rapid sequence 1 Set up the camera and obtain a properly exposed image in the DMC 2 main window Connect the remote trigger cable to the camera Click the Rapid Fire button to display the Thumbnail Gallery Press the remote trigger button to capture an image and display its thumbnail in the Thumbnail Gallery Next 40 Repeat step 4 as needed to capture and display add
20. aunch External Application e Save Viewfinder ROI settings on exit External Application Path ing the application Note The state of the Multi Frame Averaging Dark Subtract checkbox and the num ber of dark and light frames are not saved Next 69 Program Settings You Can Save Continued Preferences Box Remote Settings e Use Remote Capture e Transfer to Enhance Window e Transfer to Application e Save Directly to File Preferences Box Output Settings e Highlight property Specular or Non Specular e Color Channel Mixture Preferences Box Cameras Settings No Cameras settings are saved Remote Capture Files Folder Remote Capture Files Base Name Remote Capture Files Image Type Color Output Scale Grayscale Channel Mixture Grayscale Output Scale 70 Setting DMC 2 Software Preferences The DMC 2 Preferences dialog box Macintosh and the Set Preferences dialog box Windows let you choose these categories of settings for the DMC 2 software General e Output e Remote e Cameras To display the Preferences box for viewing or changing preferences Macintosh Windows Click the Preferences button inthe Click Set Preferences on the Tools main DMC 2 window menu 71 General Preferences Use the General tab to select general DMC 2 software operation preferences On each image capture Reset to defaults Resets all controls in the Enhance window Color Dark Lev e
21. ble hard disk space e 24 bit color display adapter e Light microscope with a 63x 1 1 or larger C mount adapter 104 Installing Software and Setting Up the Camera 1 2 3 4 5 6 Unpack Install the DMC 2 software Attach the camera to the microscope Connect the camera to the computer Connect the camera to AC power and turn on Take a test picture 105 Unpacking Make sure you have all the following components Contact your dealer if any is miss ing or damaged DMC 2 camera power supply and cord may differ from illustration for some locations SCSI host adapter installation kit for PCs optional con tact your dealer SCSI cable adapter HD50 to DB25 SCSI cable 2 meter Polaroid DMC 2 Software CD with TWAIN driver and Polaroid DMC 2 software for Windows 95 98 Me 2000 and NT Adobe Photoshop plug in and Polaroid DMC 2 software for Macintosh systems online user s guide Optional image reporting software available in some regions 106 Installing the DMC 2 Software 1 Turn the computer on On Windows systems close any applications that may be running 2 3 Insert the Polaroid DMC 2 software CD into the drive 4 Start the installation program Macintosh Windows Double click the Polaroid DMC 2 Wait for the DMC 2 install program to Installer start If it does not select Run from the Windows Start menu Click Browse and select SETUP EXE on the
22. djust color balance Adjust color balance by selecting a point that should be white Adjust color balance by selecting a point that should be neutral gray Adjust color balance by selecting a point that should be black Manually adjust color balance Manually adjust dark levels Manually adjust brightness Adjust gamma View a histogram Adjust sharpness Reduce grain in long exposures Add a label Add a calibrated micron marker Save and reuse program settings 43 Automatically Adjusting Color Balance ti To have the DMC 2 program analyze the current image and automatically set the color sliders to their optimum position click the automatic balance but ton This feature is not recommended for fluorescent imaging 44 Adjusting Color Balance by Selecting a Whitepoint Z To adjust overall color balance by identifying a whitepoint in the picture click the white eyedropper button Then click on an area of the picture that should be pure white The area you clicked becomes white and all other colors in the image are adjusted accordingly You may need to repeat the procedure to achieve an optimum white balance 45 Adjusting Color Balance by Selecting a Neutral Gray Point Z To adjust overall color balance by identifying a neutral gray point in the pic ture click the gray eyedropper button Then click on an area of the picture that should be neutral gray such as a metallic object concr
23. e enhance it with DMC 2 software then save it as a TIFF file Capture and save the image immediately as a TIFF file Capture and transfer the image to a plug in compatible application Macintosh or Capture and transfer to a TWAIN compatible application Windows 33 Capturing and Enhancing with DMC 2 Software Use this procedure to capture the image currently displayed in the DMC 2 main win dow for enhancement with DMC 2 software 1 Click Capture amp Enhance to capture the image transfer it to computer memory and display it in the DMC 2 Enhance window 2 Use the controls in the Enhance window to enhance the image characteristics add a text label or add a calibrated micron marker Instructions for enhancing the image Next 34 3 Do one of the following Macintosh Windows e Save the image to disk by clicking WM Save the image to disk by click Save Image not available when ing the Save button running the DMC 2 plug in Si Transfer the image to a TWAIN Transfer the image to a plug in compatible application by click compatible application by clicking ing the Transfer button avail Transfer Image available only able only when running the when running the DMC 2 plug in DMC 2 TWAIN source within the application 35 Capturing and Saving Immediately to Disk To capture the image and save it to disk without further enhancement 1 Click the Capture amp Save button N
24. e termination off If the camera is the only SCSI device connected to your computer or the last device in a SCSI chain set camera termination to on 1 Otherwise set termination to off 0 Termination instructions for your other SCSI devices are provided with the devices Note Do not use an external passive terminator to terminate the camera If another device is at the end of the SCSI chain that device must be terminated with an active terminator for proper camera operation Termination switch Next 114 Connecting the Camera to AC Power and Turning On 1 Connect the camera to AC power and turn the power switch on ay I I To AC power ES Power switch on 1 2 Verify that the green and yellow LEDs are on indicating AC and termination power are on Green LED Yellow LED Next 115 3 Turn the computer on 4 Wait for the system to install the camera driver and display the normal desktop Next 116 Taking a Test Picture 1 Start the DMC 2 program Macintosh Windows Double click Polaroid DMC 2 1 Select Programs from the Start menu 2 Point to Polaroid Digital Micro scope Camera 2 and select Polaroid DMC 2 2 Place a specimen under the microscope and focus it through the microscope eye piece 3 Click Preview in the DMC 2 main window If necessary adjust the c mount on the microscope to focus the image on the computer screen Refer to your micr
25. econds far left to 8 seconds far right 24 Setting Sensitivity Choose a sensitivity camera gain setting from the Sensitivity list e Low ISO 25 equivalent e Medium ISO 50 equivalent e High ISO 100 equivalent The default setting is Medium ISO 50 equivalent 25 Selecting Color Temperature You can select one of the standard color temperatures to match your lighting Tungsten 3200K Daylight 5500K These color temperatures are appropriate for many lighting situations but best results are often obtained by creating and using a custom color temperature This cus tom temperature appears as Custom in the Color Temperature list See Creating a Custom Color Temperature 26 Creating a Custom Color Temperature Setting If the standard color temperature settings are inappropriate for your lighting you can create a custom color temperature setting based on the actual lighting To create a custom color temperature Macintosh Windows 1 Select Calibrate Custom Color Tem 1 Click Calibrate Custom Color Tem perature from the Color Temp popup perature on the Tools menu MENU 2 Obtain a properly exposed and 2 Obtain a properly exposed and focused image in the DMC 2 preview focused image in the DMC 2 preview window window 3 Move the specimen to view a clear 3 Move the specimen to view a clear area of the slide area of the slide Next 27 Creating a Custom
26. en line To correct the distance draw a new line 5 Type the distance represented by the line in the Distance box 3 In the Calibrate Objectives Tips box click the Snap button The Calibrate Objective window opens and displays the micrometer image 4 Define a measured distance in the image by clicking on one micrometer mark then clicking on a second micrometer mark An x appears at each point you click Next 60 Creating a Calibrated Micron Marker continued Macintosh Windows 6 Enter or select the units of measure in To remove the second mark click the the Units popup Clear button To remove the second 7 Type a name for the marker in the and the first marks click the Clear Marker Name box button twice 8 Click Save Marker 6 Type the distance between the two marks in the Distance box and choose the units of measure for the distance End of Macintosh procedure from the Measurement Unit box 7 Type a description for this objective 30 characters max in the objective description box Next 61 Creating a Calibrated Micron Marker continued Macintosh Windows no further steps for Macintosh systems 8 If you want this calibrated marker to be the default marker in the Enhance window click the Use as default objective box 9 Click OK 62 Deleting a Calibrated Micron Marker If necessary you can delete a calibrated micron marker from the list box
27. ered by the warranty Always protect the shutter the anti aliasing filter behind the shutter and the CCD sensor by placing the cap on the camera c mount opening whenever the camera is removed from the micro scope This will also minimize the need to clean the camera optics Caution The DMC 2 camera is a precision optical instrument Carefully follow the instructions in this section when you clean it Failure to do so could damage the shut ter or the anti aliasing filter and void your warranty Next 90 Cleaning the Camera Optics Continued Verifying the Location of Dust or Dirt Avoid needless cleaning of the DMC 2 camera optics by determining the location of dust or dirt before proceeding You can determine whether the dust or dirt is in the camera optics or the microscope optics by comparing two images as follows 1 Take a picture with the camera in its normal position 2 Rotate the camera 30 or 40 degrees and take a second picture of the same speci men If the spot moves in relation to other elements in the picture it is caused by dust on the camera anti aliasing filter or the c mount adapter lens In this case skip to step 3 to determine the location If the dust remains in the same location relative to other elements in the picture it is caused by dust on the microscope optics Discontinue this procedure and refer to the cleaning instructions provided with your microscope Next 91 Cleaning the Came
28. erwrite Images During Rapid Fire 75 P package contents 106 picture taking general steps 12 picture taking sequence typical 12 power switch camera turning on 13 preferences general 72 preferences setting 71 preview choosing color or grayscale 19 preview framing and focusing 16 printing the image 42 Next 148 Index Continued R rapid fire feature using 40 rapid sequence of images capturing 40 Remote Capture Files 78 remote preferences 77 Reset to defaults 72 responding system stops solving 137 rotating the image 20 S Save Directly to a File 78 Save Viewfinder ROI settings 75 scale calibrated adding to image 58 SCSI address camera setting 111 SCSI cables connecting 113 SCSI communication problems solving 133 Next 149 Index Continued SCSI host adapter SCSI card installing 140 sections missing from image solving 136 selecting a DMC 2 camera 15 sensitivity setting 25 sequence of images capturing 40 sequence picture taking 12 service centers and offices Polaroid 125 settings program saved in settings file 68 settings program saving and reusing 64 sharpness adjusting 54 Show preview on startup 74 size in pixels image selecting 30 software updates obtaining 99 software DMC 2 starting 14 software installing 107 specifications camera 100 Next 150 Index Continued Specular 79 starting DMC 2 software 14 sync transfer rate chan
29. ete gray brick ora shadow falling on a white object The area you clicked becomes neutral gray and all other colors in the image are adjusted accordingly 46 Adjusting Color Balance by Selecting a Blackpoint E To adjust overall color balance by identifying a blackpoint in the picture click the black eyedropper button Then click on an area of the picture that should be pure black The area you clicked becomes black and all other colors in the image are adjusted accordingly 47 Manually Adjusting Color Balance Drag the three Color sliders left or right to adjust color balance The effect of your settings appears in the preview image to the left 48 Manually Adjusting Dark Levels Drag the Dark Levels slider left or right to decrease or increase dark levels The effect of your settings appears in the preview image to the left 49 Manually Adjusting Brightness Drag the Brightness slider right or left to increase or decrease brightness The effect of your setting appears in the preview image to the left 50 Adjusting Gamma Drag the Gamma slider right or left to increase or decrease gamma the apparent con trast between the highlights and the shadows The effect of your setting appears in the preview image to the left 51 Viewing a Histogram Use the histogram display to judge the distribution of brightness values in the current image The horiz
30. ge appears in the Enhance window 2 Enhance the image as needed 3 Click the full image button DN to display the image in the Full Image window 4 Use the controls in the window to zoom pan print or save the image 55 Reducing Grain in Long Exposures You can reduce image grain in long exposures longer than 500 milliseconds by enabling the DMC 2 multi frame averaging dark subtract feature To enable or disable multi frame averaging dark subtract Click Set Preferences on the Tools menu Click the General tab Click the Multi Frame Averaging Dark Subtract box to enable or disable it SA W N If you are enabling the feature select the number of light frames and dark frames Higher light frame and dark frame values usually provide more grain reduction 5 Click OK 56 Adding a Label Macintosh Windows 1 Click the Add Text Label box 1 Click the white Label strip below the 2 Type a label or other descriptive infor image mation in the text box 2 Type a label or other descriptive infor 3 Select a font and a font size mation 4 If necessary click the Bold box to change the font to bold This label appears at the bottom of the image when it is saved transferred to another application or printed 57 Adding a Calibrated Micron Marker DMC 2 software can add a calibrated scale or micron marker to the lower right corner of your image providing an accurate tool for determining
31. ging on SCSI adapters 138 system requirements 102 T technical support obtaining 132 termination SCSI setting 114 text label adding to image 57 trademark notice 124 Transfer to Application 77 Transfer to Enhance Window 77 Next 151 Index Continued U updates software 99 Use existing settings 72 Use flash 74 Use Remote Capture 77 WwW warranty camera limited 119 whitepoint selecting to adjust color balance 45 World Wide Web obtaining technical support via 132 Z zooming the preview 21 152
32. iew 15 Enhance the image 8 Set exposure 12 Turning the Camera On 1 Turn off the computer 2 Turn the camera power switch to on 1 3 Verify that the green and yellow LEDs are on indicating AC and termination power are on Green LED Yellow LED 4 Turn on the computer 13 Starting the DMC 2 Software Macintosh Windows Double click Polaroid DMC 2 1 Select Programs from the Start menu The main DMC 2 window appears 2 Point to Polaroid Digital Microscope Camera 2 and select Polaroid DMC 2 The main DMC 2 window appears If more than one DMC camera is connected to the computer you must select the cam era to be used in this session To select a camera see Selecting a DMC Camera 14 Selecting a DMC Camera If more than one DMC camera is connected to the computer use these steps to select the camera to be used in the current session Macintosh Windows 1 Click the Preferences button in 2 the main DMC 2 window 2 Click Choose a DMC 2 in the DMC 2 Preferences box 3 Choose the SCSI address of the camera to be used 4 Click OK to activate the camera 5 Click Save to close the DMC 2 Preferences box 1 Click Set Preferences on the Tools menu 2 Click the Cameras tab 3 Click the SCSI address of the camera to be used 4 Click OK 15 Framing and Focusing the Preview 1 View the subject through the microscope eyepiece 2 Adjust
33. ions for setting termination Low quality cables Use only high quality SCSI cables Cables are too long Use shorter SCSI cables Standard SCSI chains are limited to a total length of 6 meters Fast SCSI chains are limited to 3 meters Faulty device termination power fuse Return device for service see technical support 136 Symptom System stops responding during image transfer If your system locks and stops responding to the mouse and keyboard during image transfer check the following Incorrect maximum sync transfer rate or other settings for Adaptec 2940 and 3940 host adapters only Change the maximum sync transfer rate to its lowest set ting 5 0 or 10 0 depending on the adapter model See Changing Sync Transfer Rate on Some Adaptec SCSI Adapters Device on same SCSI bus with hard drive or CD ROM drive Install a second SCSI host adapter for connecting the device See Installing a SCSI Host Adapter 137 Changing Sync Transfer Rate on Some Adaptec SCSI Adapters If your system has an Adaptec 2940 or 3940 SCSI host adapter it may not recognize a SCSI device if the minimum sync transfer rate or other values are incorrectly set To change the minimum sync transfer rate and other important values for these SCSI adapters 1 Restart your computer and wait for the following message to appear Press lt Crtl gt lt A gt for SCSISelect TM Utility 2 Immediately press the Control key and A t
34. itional thumbnails until the camera buffer is full Note Change the Rapid Fire setting on the General tab of the Set Preferences box to stop capturing images when the buffer is full or to begin overwriting the first images captured Right click a thumbnail you want to transfer A shortcut menu appears Click Image Output Size and select an image size To display the image in the Enhance window for image enhancement before sav ing right click the thumbnail again and click Enhance Then adjust color bright ness gamma etc as usual and save the image To save the image immediately right click the thumbnail and click Save 41 Printing the Image To print the current image from the DMC 2 Enhance window Macintosh Windows Select Print from the File menu Click the print button to open your printer dialog box Choose the appropriate printer settings and click OK The image prints at 300 dpi and sized to fill the page Note To print at different resolutions or sizes save the image and open it with an image editing application such as Adobe Photoshop 42 Enhancing the Image with DMC 2 Software After you capture an image by clicking Capture amp Enhance in the DMC 2 main win dow the captured image is stored in memory and displayed in the DMC 2 Enhance window Before printing saving or transferring this image to another application you can apply the following enhancements Automatically a
35. le Optimizes the output image tone values for display on a monitor Linear Sets the tone value of each output pixel in proportion to the actual transmittance or reflectance measured by the camera Logarithmic Sets the tone value of each output pixel in proportion to the logarithm of the actual transmittance or reflectance measured by the camera 84 Camera Preferences Use the Select Camera tab to select the active DMC camera when multiple DMC cam eras are connected to the computer Cameras are identified by their Device IDs SCSI addresses and their Adapter IDs To select a camera click the appropriate line in the window and then click OK 85 Using the Color Stacker Utility The Color Stacker utility provided with DMC 2 software provides a way to combine three images This is useful when imaging a single sample with multiple fluoro chromes Instead of viewing separate images you can process the fluorochromes in red green and blue channels and view the result in a single composite image Creating a Composite Image Macintosh Windows 1 Use the DMC 2 camera and softwareto 1 Use the DMC 2 camera and software to capture and save an image for each of capture and save an image for each of the three color channels You must the three color channels You must save the images in the TIFF format save the images in the TIFF format Next 86 Creating a Composite Image Continued Macintosh
36. located below the Add micro marker box 1 Click Add micron marker at the bottom of the Enhance window Click Delete Micron Marker from the list of micron markers In the Micron Marker box click the name of the micron marker you want to delete Click Delete 5 To remove additional micron markers repeat steps 3 and 4 Click Close 63 Saving and Reusing Program Settings If you develop settings in the Enhancement window that you expect to use frequently you can save them to a file then reload them when necessary If necessary see Program settings you can save To save the current program settings in the current settings file Macintosh Windows 1 In the Enhancement window click In the Enhancement window click Save Settings Settings on the File menu 2 Select Save Settings Next 64 Saving and Reusing Program Settings continued To save the current settings in a new settings file Macintosh Windows 1 In the Enhancement window click 1 If the main DMC 2 window is open click Settings Settings on the File menu to open the 2 Type a name for the new settings Settings window Then click New to dis file play the Add New Settings box 3 Select Save If the Enhance window is open click Save Settings As on the File menu to display the Add New Settings box 2 In the Settings Name box type a descriptive name for the new settings you are saving 3 Click OK Next 65
37. lower Distilled water or methanol 3 Detach the camera from the microscope and remove the c mount adapter from the camera If you are using the camera with a lens remove the lens 4 Ifthe camera is not connected to the computer turn off the camera and the com puter Then connect the camera to the computer 5 Turn on the camera then turn on the computer and wait for it to start Next 94 Cleaning the Camera Optics Continued 6 Run the shutter opening software Macintosh Windows a Double click DMC 2 Shutter a Select Programs from the Windows Opener Start menu b Click Open DMC 2 Shutter b Point to Polaroid Digital Micro c Click OK scope Camera 2 and select Shutter Cleaning Tool 7 Power down the computer and turn the camera power off The shutter remains open Next 95 Cleaning the Camera Optics Continued 8 Clean the anti aliasing filter behind the shutter with air If necessary gently dis lodge any dust with the camel hair brush Caution Use care when inserting anything into the camera lens opening The shutter blades and filter are easily damaged If using canned air be sure to hold the can upright to avoid spraying liquid propellant into the camera Also avoid spraying canned air directly at the shutter 9 Ifthe filter is clean skip to step 10 If dust or dirt remains after you use the air and the brush follow these steps a Moisten a lint free wipe with distilled water or
38. ls Brightness Gamma Sharpness to their factory defaults after you capture a picture Use existing settings Leaves all controls in the Enhance window unchanged after you capture a picture Do auto color balance Automatically analyzes the image and sets the Dark Levels and Brightness sliders in the Enhance window to their optimum values each time you click the Capture amp Enhance button or the Capture amp Transfer button in the Main window Also sets the Enhance window color sliders to 0 sets the Gamma slider to 50 and applies the color cast setting selected under Cast found on auto color balance described below Next 72 General Preferences Continued Cast found on auto color balance available only if Do auto color balance is selected under On each image capture Remove it Removes any overall color cast tint from the image when per forming auto color balance Keep it Retains any overall color cast in the image when performing auto color balance Always ask If an overall color cast is detected during auto color balance dis plays a dialog box for removing or keeping it Next 73 General Preferences Continued Show preview on startup Expands the Main window to show a preview image when the DMC 2 program starts Use flash Activates the x sync flash cable connector on the DMC camera when the image is captured The default setting is off disabled X sync flash cable connector Ne
39. me jurisdictions do not allow the exclusion or limitation of consequential damages so the foregoing exclusion may not apply to you 119 Radio and Television Interference FCC Notice U S A Note This equipment has been tested and found to comply with the limits for a Class B digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference in a residential installa tion This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful inter ference to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful inter ference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the following measures e Reorient relocate the receiving antenna e Increase the separation between the equipment and receiver Next 120 FCC Notice continued e Connect the equipment into an outlet on a circuit different from that to which the receiver is connected Consult the dealer or an experienced radio TV technician for help Note If your camera SCSI cable has a ferrite core on one end that end must be con nected to the camera FCC Caution To assure
40. methanol b Lightly wipe across the surface of the filter in a single direction only Note Do not go over a previously wiped area with the same wipe and do not reuse a wipe Dirt collected on the wipe can damage the filter Next 96 Cleaning the Camera Optics Continued c If dust or dirt remains after the first attempt to wipe it away repeat the proce dure with a new clean wipe 10 After the filter is clean close the shutter by turning the camera power switch on and then off 11 Reattach the camera to the microscope 97 Cleaning the Camera Body If the DMC 2 camera body requires cleaning use a clean soft cloth dampened with water Do not use cleaners of any kind and do not spray water or other liquids directly onto the camera 98 Obtaining Software Updates For the latest information about DMC 2 software updates visit the Polaroid Web site www polaroid com 99 DMC 2 Camera Specifications Sensor Output resolution Image formats Image transfer speed Computer interface Weight Next 2 1 megapixel 11mm CCD Super high resolution 1600x1200 pixels High resolution 800x600 Normal resolution 400x300 pixels Windows version only 48 bit color 68 7 billion colors 24 bit color 16 million colors 16 bit grayscale 65 000 gray shades 8 bit grayscale 256 gray shades Greater than 2 MB second SCSI data transfer SCSI 2 2 1 Ib 963g 100
41. nd Reusing Program Settings Program Settings You Can Save Next 47 48 49 50 io 152 54 ao 56 ioe 58 59 63 64 68 Setting DMC 2 Software Preferences General Preferences Remote Preferences Output Preferences Camera Preferences Using the Color Stacker Utility Creating a Composite Image Color Stacker Preview Options Camera Maintenance Cleaning the Camera Optics Cleaning the Camera Body Obtaining Software Updates DMC 2 Camera Specifications Next 71 TE Dee 79 a D 86 86 88 89 90 98 100 System Requirements PC Minimum System Requirements Macintosh Minimum System Requirements Installing Software and SE Up the Camera Unpacking Installing the DMC 2 sofware e Attaching the Camera to the Micrekiape Connecting the Camera to the Computer Connecting the Camera to AC Power and Turning On Taking a Test Picture Camera Limited Warranty Radio and Television Interference Copyright and Trademark Notices Next 120 124 Polaroid Offices and Service Centers Technical Support Solving SCSI Communication Problems Changing Sync Transfer Rate on Some Adaptec SCSI Adapters Installing a SCSI Host Adapter Index 125 132 133 138 140 141 Navigating in This Document This Polaroid electronic document uses the Adobe Portable Document Format
42. o display the SCSISelect menu 3 Select Configure View Host Adapter Settings Next 138 Make sure the SCSI Device Settings are as follows Setting Optimum Value Initiate Sync Negotiation No Maximum Sync Transfer Rate Minimum 5 0 or 10 0 Enable Disconnection No Initiate Wide Negotiation No Note Change these settings only for the SCSI address of the device not recognized Leave all other settings unchanged Press Esc until you are prompted to save the changes Select Yes then press the space bar to restart the computer 139 Installing a SCSI Host Adapter This device connects to a SCSI host adapter in your computer Most Macintosh com puters have a SCSI adapter built in but most IBM PC compatible computers do not If you must install a SCSI adapter turn the computer off and follow instructions pro vided by the adapter manufacturer The procedure usually includes software installa tion If your PC uses the Windows NT operating system log in as an administrator before installation Note A SCSI host adapter may have been provided as an option with your hardware device If necessary obtain an adapter from your computer dealer Adapters are not available for iMac computers 140 Index A AC power connecting camera to 115 area of interest choosing 17 attaching camera to microscope 108 B blackpoint adjusting color balance by selecting 47 brightness adjusting manually 50 C
43. ontal axis represents all possible brightness values in the image 0 255 and the vertical scale represents the number of pixels at each brightness value The Channel list lets you select a channel Luminosity Red Green Blue RGB for the histogram The histogram changes to reflect adjustments in the Exposure setting in the DMC 2 main window and other controls in the Enhance window How to view a histogram 52 To view a histogram of brightness levels in the image 1 If the DMC 2 main window is visible click Histogram on the Tools menu If the Enhance window is visible click the histogram button Wal Select a channel for display from the Channel list Luminosity Red Green Blue RGB If necessary adjust the Exposure slider in the main DMC 2 window or adjust the controls in the Enhance window while viewing their effect on the histogram 53 Adjusting Sharpness Drag the Sharpness slider left or right to decrease or increase sharpness The effect of your setting appears in the preview image to the left 54 Viewing the Full Image If necessary you can view the full image for close inspection after capturing but before saving When viewing the full image you can zoom and pan to assure you have captured the desired area and detail and that enhancements you applied have the expected results To view the full image 1 Inthe main DMC 2 window click Capture amp Enhance The captured ima
44. or Stacker utility 86 focus verifying digitally 32 focusing and framing preview 16 frame smaller choosing for capture 17 framing and focusing preview 16 full image viewing 55 Next 144 Index Continued G gamma adjusting 51 grain reducing in long exposures 56 gray point selecting to adjust color balance 46 Grayscale Channel Mixture 82 Grayscale Output Scale 84 grayscale preview choosing 19 H Highlight Property 79 histogram viewing 52 horizontal lines solving 136 Next 145 Index Continued l image enhancement 43 image size in pixels selecting 30 image type selecting 29 installing software and setting up camera 105 interference radio and TV 120 Internet obtaining technical support via 132 ISO value setting 25 L label adding to image 57 Launch External Application 76 launching DMC 2 software 14 lines horizontal solving 136 lockup system solving 137 Next 146 Index Continued M maintenance camera 89 marker calibrated adding to image 58 micron marker calibrated adding 58 micron marker calibrated creating 59 micron marker deleting 63 missing image sections solving 136 multi frame averaging using to reduce grain 56 Multi frame Averaging Dark Subtract 75 N navigating in this document 7 Non specular 79 Next 147 Index Continued O offices and service centers Polaroid 125 Output Defaults 80 output preferences 79 Ov
45. oscope manual for instructions Next 117 In the DMC 2 main window choose the appropriate settings for the image being captured Exposure Sensitivity Color Temperature Select 8 Bit Color from the Image Type box for the test Click Capture amp Save to capture and save the image to disk Open the saved image with an image analysis application and verify that it was captured satisfactorily Note The color balance of this test image may not be satisfactory because of vari ations in light source color temperatures You can correct this problem by creating and using a custom color temperature setting See Creating a Custom Color Tem perature Next 118 Camera Limited Warranty Polaroid Corporation warrants the DMC 2 camera against defects in manufacture or work manship for a period of one year from the date of purchase To verify the warranty period you should keep the invoice sales receipt or other proof of the purchase date Should this product or any component or accessory included with it with the exception of software prove to be defective at any time during the warranty period Polaroid Corporation will at its discretion either replace or repair this item without charge This warranty does not cover damage caused by accident incorrect installation unauthorized modification and misuse or abuse A charge will be made for repair of such damage This warranty excludes all consequential damages So
46. ote On Macintosh systems this option is available only when running the DMC 2 software When running the DMC 2 plug in within another application click Capture amp Transfer Then save the image within the other application Choose a folder and filename for the image Click Save 36 Capturing and Transferring to Another Application Macintosh Note This option is available only if you are running the DMC 2 plug in within the other application It is not an option if you are running the DMC 2 program To capture the image and display it in the application click the Capture amp Transfer button and wait for the image to appear in your application After the image appears you can edit print or save it according to instructions pro vided with the application 37 Capturing and Transferring to Another Application Windows Use these steps if you want to transfer the captured image immediately to another image processing or image analysis program The procedure you use depends on whether you opened the DMC 2 program from the Windows Start menu or you opened the DMC TWAIN software by selecting Acquire from an application s File menu If you opened the DMC 2 program from the Windows Start menu 1 2 3 4 In the DMC 2 main window click Set Preferences on the Tools menu Click the General tab Click the Launch External Application box to enable it In the External Application Path box type the path and n
47. ra Optics Continued 3 Follow these steps to determine whether the dust spot is on the camera optics or the c mount adapter lens a Separate the camera from the c mount b Install the lens cap on the DMC 2 camera c Clean the c mount adapter lens according to the manufacturer s instructions d Reattach the c mount and the DMC 2 camera to the microscope and take a pic ture e Ifthe spot remains in the image follow the camera optics cleaning steps If the spot is no longer visible discontinue this procedure Next 92 Cleaning the Camera Optics Continued Camera Optics Cleaning Steps 1 Ifyou have not already done so install the shutter opening software on your com puter as follows This software opens the shutter allowing you to clean the cam era optics Macintosh Windows a Copy the file DMC 2 SHUTTER No special installation is necessary OPENER INSTALLER from the DMC The shutter opening software is 2 CD provided with the camera to installed during DMC 2 software the desktop installation b Double click DMC 2 Shutter Opener Installer c Follow instructions on the screen Next 93 Cleaning the Camera Optics Continued 2 Obtain the following cleaning materials e Clean camel hair brush small e Lint free disposable cleaning wipe such as e wipe or a high grade lens Can of clean room grade com e tissue non abrasive and lint free pressed air Bulb type manual b
48. rpness Transfer to Application Captures images and automatically transfers the image to an image editing program Next 77 Remote Preferences Continued Save Directly to a File Captures images and saves them directly to a file for viewing and enhancing at a later date Remote Capture Files Change Allows you to specify a location for your image files if you select Save Directly to a File Also allows you to select the file format TIFF BMP or JPEG and the base name of the saved files 78 Output Preferences Use the Output tab to select the highlight property and the output defaults of the images Highlight Property Specular Usually provides best image results with reflected light microscopy specimens containing specular highlights reflective or metallic areas or points much brighter than most of the image Non specular Usually provides best results for reflected light microscopy without specular highlights and for all transmitted light microscopy Next 79 Output Preferences Continued Output Defaults For best results choose the appropriate channel mixture and output scale for your specimen and the way the image will be used Color Channel Mixture CRT color Optimizes the output image color for display on a monitor Identity Leaves the output image color as detected by the camera Portrait Optimizes the output image color for studio portrait photogra phy software programs
49. subject position focus and lighting according to the microscope instruc tions 3 Ifyour microscope requires manually switch the light path to the camera If a viewfinder image is not visible in the DMC 2 software main window click the Preview button Note If clicking Preview does not display an image you may need to adjust expo sure or sensitivity before continuing Exposure adjustment instructions Sensitivity adjustment instructions 16 Choosing a Capture Area You can capture the entire DMC frame or a smaller selected area To capture an area smaller than the DMC frame 1 Adjust exposure to obtain an acceptable image on the Viewfinder Display tab of the main DMC 2 window Click the Region of Interest Selection tab Select the size of the frame to be captured 3 4 or 1 2 frame from the Capture Area list A box defining the selected capture area appears on the image 4 Position the capture area as desired by dragging it Next 17 5 Click the Viewfinder Display tab to view only the capture area The values avail able in the Image Size box correspond to the capture area you selected 6 Continue with the picture taking sequence 18 Choosing Color or Grayscale Preview You can view the preview image in the DMC 2 main window in either color or gray scale A color preview provides a more accurate representation of the final image while a grayscale preview refreshes faster To
50. xt 74 General Preferences Continued Save Viewfinder ROI settings When the DMC 2 program is closed saves the image capture area region of interest selected on the Region of Interest Selection tab in the DMC 2 main window Overwrite Images During Rapid Fire When camera buffer becomes full during a rapid fire sequence begins overwriting the first file in the buffer instead of stopping rapid fire Multi frame Averaging Dark Subtract Enables multi frame averaging and dark frame subtract for reducing grain in long exposures longer than 500 milliseconds After enabling this feature select the number of light frames and dark frames Higher values increase grain reduction Next 75 General Preferences Continued Launch External Application Starts the image editing application specified in the External Application Path box when an image is saved to disk Displays the saved image in the application 76 Remote Preferences Use the Remote tab to capture images using the remote trigger You can specify if you want the captured images transferred to an image editing program the Enhance win dow or to files Use Remote Capture Captures images using to remote trigger Select if you want to capture multiple images remotely Transfer to Enhance Window Captures images and automatically transfers them to the Enhance window where you can manually adjust color balance dark levels or brightness gamma or sha
51. yright 2001 Polaroid Corporation Cambridge MA 02139 USA Polaroid is a registered trademark Polaroid Corporation All other product names may be trademarks of their respective owners All rights reserved 124 Polaroid Offices and Service Centers Australia Polaroid Australia Pty Ltd 13 15 Lyonpark Road P O Box 163 North Ryde NSW 2113 Tel 02 950 7000 Fax 02 887 2209 Belgique Belgi Polaroid Belgium N V S A Rue Colonel Bourg 111 Kolonel Bourgstraat 111 1140 Bruxelles 1140 Brussel Tel 027143102 Flemish Tel 027143106 French Next Espana Polaroid Espa a S A Calle Orense 16 2 planta 28020 Madrid Tno 913778103 France Polaroid France S A B P 7 78996 Elancourt C dex Tel 0141913810 125 Brasil Polaroid do Brasil Ltda Av Paulista 1776 11 andar Cerqueira Cesar Sao Paulo Capital 01310 921 Tel 55 11 285 6411 Fax 55 11 283 2625 Fax 55 11 288 6521 Fax 55 11 287 5393 Canada Polaroid Canada Inc 250 Carlingview Drive Etobicoke Ontario M9W 5G6 Toll free 1 800 268 6970 Next Hong Kong Polaroid Far East Ltd 32 F Windsor House 311 Gloucester Road Causeway Bay Tel 852 2894 0333 Ireland Tel 014026897 126 Danmark Polaroid a s Blokken 75 3460 Birkerod Tlf 80011887 Deutschland Polaroid GmbH Sprendlinger Landstrasse 109 63069 Offenbach Tel 06995096056 Next Italia
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