PASCO Specialty & Mfg. PS-2106 User's Manual
Contents
1. T 60 3 40 G 20 Timed min 2 2 4 6 8 10 12 14 16 18 32 DataStudio DS Lite Tasks View a measurement From the Data list drag the run icon to the open display Change measurement units On the main toolbar click on the Setup S button Scroll to the Dissolved Oxygen Sensor options box and click to select the desired unit from the unit drop down menu Scale to fit the data On the graph toolbar click on the Scale to Fit 2 button View data statistics In the Graph display click on the Statistics z171 button and select Minimum Maximum or Mean 012 07688C CARD 1B Equipment Required Dissolved Oxygen Sensor with electrode storage bottle PS 2108 Temperature Sensor PS 2125 or a thermometer PASPORT interface Xplorer PS 2000 USB Link PS 2100 etc DataStudio software Cl 6870C 400 mL distilled or deionized water Sugar Sucrose 5 grams 5 mL activated yeast suspension follow directions on yeast package Clamps and lab stand to suspend the 2 sensors in solution Lab glassware 1 L bottle with lid 6 00 mL and 400 mL beakers graduated cylinder small test tube stirring rod Wash bottles for rinsing sensors Connect the Dissolved Oxygen Sensor to a USB Link or Xplorer and launch DataStudio Fill a 1 liter bottle with 400 mL of distilled water Tightly close the lid and shake vigorously for 10 seconds to oxygenate the water2. Pour the oxygenated water into the 600 ml beaker and dissolve five grams of sugar into it Put the Temperature Sensor and Dissolved Oxygen Sensor into the sugar solution and gently stir the solution Click the Start Stat button to begin collecting data Continue stirring After 30 seconds add the 5 mL sample of activated yeast suspension to the beaker Continue stirring and recording data Record data until the dissolved oxygen level stabilizes approximately 10 minutes then click the Stop s button If time permits perform additional runs with oxygenated water that is a few degrees colder or warmer than room temperature CARD 2A Equipment required 1 PASPORT Dissolved Oxygen Sensor PS 2108 1 electrode storage bottle included with sensor DataStudio software version 1 5 3 or higher or a PASPORT Xplorer PS 2000 and deionized water Optional Barometer PS 2113 and Temperature Sensor PS 2125 for performing calibrations at a specific barometric pressure and temperature Note After plugging in the Dissolved Oxygen Sensor you must allow 2 minutes for the Figure 1 electrode Shake bottle vigorously for about 10 seconds sensor to come to equilibrium Equilibrate the Probe in 100 Humidified Air 1 Before performing any calibration place 5 mL of deionized water into a clean electrode storage bottle Loosen the lid to the electrode storage bottle Insert the probe into the elect
3. here gt Syringe tip should be close to but not touching Cartridge with the membrane membrane 012 07688C CARD 3B The O ring rarely requires replacing However if it develops nicks Short term storage and splits or leaks electrolyte solution from the probe replace it as 1 Rinse the probe covering and tap or shake gently to dry follows 2 Empty the electrode storage bottle and rinse it if necessary 7 3 Insert the clean dry probe into the clean empty electrode storage bottle 1 POPON SIEPA E A UNGEN RAPACI WE Men aS 4 Enclose the probe with bottle in the plastic zip lock storage bag Note Keeping 2 After removing the membrane cartridge remove the O ring with a pair of fine tipped tweezers and insert a new O ring 3 Reassemble the unit as directed in steps 4 7 of Changing the Electrolyte Solution the end of the probe in the empty storage bottle protects the probe membrane from trauma Long term storage 1 Empty the electrolyte solution in the cartridge housing and clean the electrode before storage Note It is not necessary to store the probe in water or solution for long term storage Follow the procedure on card 3A for Changing the Electrode Solution except for refilling the cartridge housing 2 Clean any tarnish oxidation off of the electrode and replace the dry cartridge housing cartridge ie 3 Insert the probe in the empty electrode storage bottle The sensor is ready for sto
4. Dissolved Oxygen Sensor pasfol CARD 1A The PS 2108 Dissolved Oxygen Sensor measures the amount of dissolved oxygen mg liter or in a solution PS 2108 e PASPORT interface USB Link Xplorer etc e EZscreen or DataStudio software version 1 5 3 or later Sensor Specifications 1 Connect the PASPORT interface to a USB port on your computer or to a USB hub 2 Connect the Dissolved Oxygen probe to the sensor Sensor Range 0 mg L to 20 mg L 3 Connect the sensor to the PASPORT interface If using an Xplorer in the classroom Accuracy 10 of full scale connect the Xplorer cable to a USB port or hub The software launches when it detects a PASPORT sensor Resolution 0 01 mg L 4 Select a choice from the PASPORTAL window Max Sample Rate 20 samples per second 9 3 Default Sample Rate 2 samples per second q _ Response 98 in 60 seconds Temperature Range 0 C to 50 C 4 Temperature Compensation 10 C to 40 C Cathode Platinum Note Prior to first use fill the electrode o Anode Ag AgCl membrane cartridge and housing with a gt gt electrolyte solution See Changing the Electrolyte Solution on card 3A oy Koy oT 0 800 772 8700 916 786 3800 techsupp pasco com www pasco com Effect of Cellular Respiration on Dissolved Oxygen Concentration HEN za de Xj A Data gt i I j emperature Deg C eo Time minutes 2 14 16 1
5. c stir bar if available Avoid placing the sensor in acidic or caustic solutions or vapors The sensor was designed for use in aqueous solutions Placing the sensor in caustic abrasive or harsh solutions may damage the membrane and or probe When taking measurements do not drag the probe or allow the membrane to touch the bottom of a container or body of water The membrane is fragile and may become damaged or pop out with mishandling We do not recommend using the the Dissolved Oxygen Sensor in environments with rapidly changing temperature conditions The sensor is temperature compensated but large temperature changes may give false readings e Avoid using the sensor in solutions with high salinity such as ocean water Check for large water drops You can calibrate the Dissolved Oxygen in the field or connected to DataStudio For a percent saturation calibration 1 Turn on the Xplorer 2 Plug the sensor into the Xplorer 3 Press the Display 6 button until Calibrate appears on the screen 4 Press the Plus button and select as the measurement unit 5 Put the Dissolved Oxygen Sensor in water that is 100 oxygen saturated 6 Check the Xplorer display When the value displayed settles press the Check Q button 7 Quickly press the Check button again to accept the new calibration 8 To exit calibration mode press the Display button Note To calibrate in mg L instead of see our web site for do
6. cumentation on our dissolved oxygen sensors and or contact PASCO s Technical Support Dept 012 07688C CARD 2B Factors that may affect dissolved oxygen measurements include atmospheric pressure atmospheric temperature air density altitude and the salinity in the sample If you plan to take quantitative dissolved oxygen measurements in mg L instead of percent saturation calibrate the sensor in mg L units A mg L calibration requires that you mathematically adjust for atmospheric pressure atmospheric temperature and altitude Note The percent saturation measurement is recommended for comparative studies or when examining relative changes in dissolved oxygen concentration Calibration tables for mg L units of dissolved oxygen can be found in the following reference Hitchman Michael L 1978 Measurement of Dissolved Oxygen In Chemical Analysis volume 49 edited by Elving and Winefordner 196 199 New York John Wiley and Sons Inc CARD 3A To maintain optimal performance of the probe periodically replace the electrolyte solution and clean the silver electrode Replace the electrolyte solution and clean the electrode as follows 1 Hold the probe below the stainless steel band unscrew the probe and remove the cartridge housing Note Always hold the probe below the stainless steel band when unscrewing or applying torque to the probe 2 Rinse and air dry the cartridge housing 3 Rinse the electrode with deioniz
7. ed water Examine the silver portion of the electrode if it appears tarnished rub this portion with a paper towel to remove the oxidation Be careful not to rub the glass portion of the electrode 4 Fillthe syringe with 10 ml of electrolyte solution the solution provided with the sensor Avoid air bubbles 5 Place the tip of the syringe very close to but not touching the membrane and If the membrane is damaged replace it as follows 1 Follow steps 1 3 under Changing the Electrolyte Solution 2 Use the plunger to push the steel cylinder membrane cartridge out of the cartridge housing Figure 1 3 Examine the O ring and replace it if it is damaged See Replacing the O ring and Figure 3 on the reverse side of this card 4 Use the plunger to insert a replacement membrane cartridge and push it down until it is seated at the end of the housing that has the O ring Figure 2 5 Follow steps 4 7 of Changing the Electrolyte Solution slowly fill the membrane cartridge and cartridge housing to approximately 5 mm from the top of the housing Note Do not allow air bubbles to form in the cartridge housing or membrane 6 Agitate the cartridge housing after filling to dislodge air bubbles 7 Replace the cartridge housing and dry the sides with a paper towel Do not touch the membrane plunger Figure 1 stainless steel J O ring i aes band on TE cartridge T cartridge ae cartridge 7 A O ring
8. rage Figure 3 If the Dissolved Oxygen Sensor does not give the expected output perform the three maintenance procedures described on this card checking the sensor operation after each procedure Examine the O ring for damage 1 Changing the Electrolyte Solution 2 Replacing the Membrane 3 Replacing the O ring If the Dissolved Oxygen Sensor still does not function properly contact PASCO Technical Support
9. rode storage bottle and screw on the lid Place the end of the probe about 2 cm above the water See Figure 1 Vigorously shake the electrode storage bottle for about 10 seconds to saturate the water Shake off any large water drops from the membrane N 9 For a percent saturation calibration 1 Put the Dissolved Oxygen Sensor in water that is 100 oxygen saturated See the section above In DataStudio click the Setup 52 button to open the PASPORT Setup window In the Setup window click on the pull down menu to ensure the percent saturation unit is selected from the Dissolved Oxygen Sensor options Click the Calibrate Calibrate button The default is 100 saturation When the Calibration window opens the current value displays under Dissolved Oxygen 5 When the current value settles click the Set and OK buttons Note Fora mg L calibration see our web site www pasco com for documentation on our Dissolved Oxygen sensors and or contact PASCO s Technical Support Dept e Always change the filling solution before calibrating the Dissolved Oxygen sensor or when taking your first set of measurements with the sensor e For best results always stir the solution before taking measurements When using the Dissolved Oxygen Sensor it is important to move a steady flow of test solution past the sensor s membrane while measuring the sensor uses up 0 molecules faster than they can diffuse back into the area Use a magneti
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