Omega Engineering Slow Cooker 48 User's Manual
Contents
1. Shop online at omega com N 0MEGA omega com e mail info omega com For latest product manuals omegamanual info TCY 20 25 30 amp 48 Thermal Cyclers omega com OO OMEGA OMEG Anet Online Service Internet e mail omega com info omega com U S A ISO 9001 Certified Canada Servicing North America Omega Engineering Inc One Omega Drive P O Box 4047 Stamford CT 06907 0047 USA Toll Free 1 800 826 6342 TEL 203 359 1660 FAX 203 359 7700 e mail info omega com 976 Bergar Laval Quebec Canada H7L 5A1 Toll Free 1 800 826 6342 TEL 514 856 6928 FAX 514 856 6886 e mail info omega ca For immediate technical or application assistance U S A and Canada Sales Service 1 800 826 6342 1 800 TC OMEGA Mexico Benelux Czech Republic France Germany Austria United Kingdom ISO 9001 Certified Customer Service 1 800 622 2378 1 800 622 BEST Engineering Service 1 800 872 9436 1 800 USA WHEN En Espafiol 001 203 359 7803 FAX 001 203 359 7807 info omega com mx e mail espanol omega com Servicing Europe Managed by the United Kingdom Office Toll Free 0800 099 3344 TEL 31 20 347 21 21 FAX 31 20 643 46 43 e mail sales omega nl Frystatska 184 733 01 Karvina Czech Republic Toll Free 0800 1 66342 TEL 420 59 6311899 FAX 420 59 6311114 e mail info omegashop cz Managed by the United Kingdom Office Toll Free 0800 466 342 TEL 332. from the menu and press Enter 2 STEP 23 3 STEP 33 AV d d to select EEE Give the program a name and edit any of UNNAMED the default fields as described above Heated Lid LOS C Preheat Lid on My to edit Vv to save To add a stage and then edit it Use the Down Arrow NY key to scroll to In denat 940 C Emdi the dotted line below Hot Start it will start to flash Hot Start off eee eee ee ee eee eee ee Press Enter to insert a stage 20 Number cycles Step 94 0 C 00m30 max amp edit no cycles Number cycles 94 0 C 00m30 max Step r I to insert step d a to edit to delete step A new stage will be inserted which consists of a Number cycles field and one step Edit the number of cycles by typing the number required then press Enter Use the Down Arrow key to move to the step Press Enter to edit the step as described above Press Enter d again to accept To insert additional steps Step a les 94 0 C OOms3 max to insert step TL te edit To delete a step Number cycles Step I 94 0 C 00m30 max to insert step d to edit to delete step Number cycles Step 94 0 C 00m30 DEL STEP a Yes or W No max To insert a new step before the first step use the Up Arrow or Down Arrow keys to select Number cycles
3. under 10 CFR 21 NRC used in or with any nuclear installation or activity or 2 in medical applications or used on humans Should any Product s be used in or with any nuclear installation or activity medical application used on humans or misused in any way OMEGA assumes no responsibility as set forth in our basic WARRANTY DISCLAIMER language and additionally purchaser will indemnify OMEGA and hold OMEGA harmless from any liability or damage whatsoever arising out of the use of the Product s in such a manner RETURN REQUESTS INQUIRIES Direct all warranty and repair requests inquiries to the OMEGA Customer Service Department BEFORE RETURNING ANY PRODUCT S TO OMEGA PURCHASER MUST OBTAIN AN AUTHORIZED RETURN AR NUMBER FROM OMEGA S CUSTOMER SERVICE DEPARTMENT IN ORDER TO AVOID PROCESSING DELAYS The assigned AR number should then be marked on the outside of the return package and on any correspondence The purchaser is responsible for shipping charges freight insurance and proper packaging to prevent breakage in transit FOR WARRANTY RETURNS please have the FOR NON WARRANTY REPAIRS consult OMEGA following information available BEFORE for current repair charges Have the following contacting OMEGA information available BEFORE contacting OMEGA 1 Purchase Order number under which the product 1 Purchase Order number to cover the COST was PURCHASED of the repair 2 Model and serial number of the product under 2 Model and s
4. 0 161 37 29 00 FAX 33 0 130 57 54 27 e mail sales omega fr Daimlerstrasse 26 D 75392 Deckenpfronn Germany Toll Free 0 800 6397678 TEL 49 0 7059 9398 0 FAX 49 0 7056 9398 29 e mail info omega de OMEGA Engineering Ltd One Omega Drive River Bend Technology Centre Northbank Irlam Manchester M44 5BD England Toll Free 0800 488 488 TEL 44 0 161 777 6611 FAX 44 0 161 777 6622 e mail sales omega co uk It is the policy of OMEGA Engineering Inc to comply with all worldwide safety and EMC EMI regulations that apply OMEGA is constantly pursuing certification of its products to the European New Approach Directives OMEGA will add the CE mark to every appropriate device upon certification The information contained in this document is believed to be correct but OMEGA accepts no liability for any errors it contains and reserves the right to alter specifications without notice WARNING These products are not designed for use in and should not be used for human applications Contents Ac ATIEFOGU GUO IN ois nrn aaae a aara oariad iani 4 2 Safety and INSTAlaNoNn 2 eee 4 3 Cycler TG Y SPECMIEANON ae 7 4 User operation and consumables anxrannnnnnnnnnnnnnnnnnennnnnnnnnnnnennnennnnnnnunnnnennnennnunnn 8 FT MUS 6 LO ME EN A EE EE OE NE EE NE emp meanaeeed 8 TPOUDES OF reaction vessels sje eek 8 O Smi gt EE EEE 9 SVEN Naval 10 9 Front panel CONOIS LEE 11 TELE PD DENE uer See 11 g 0 ALOIS ssa oa e ENE
5. Alpha Numeric keys The Alpha Numeric keys are used to enter numbers and letters during programming If it is a numeric field then numbers will be inserted if it is an alpha field then letters gt MD will be inserted Each key will cycle through the letters shown on the key 0 will insert a Oo space To insert a number press and hold the appropriate key 6 Programming the CYCLER The table below illustrates the symbols which appear on the LCD display and the relevant keypad button to which they refer 13 In the prompt line is equivalent to the End key can be copied and then edited but not edited directly Up arrow Down arrow I On a program line means the program is locked it lt Enter Pause The following terms are used when programming the CYCLER TCY Stage A Stage is the name given to the main repeated part of a program containing the Steps to be repeated A program can contain a maximum of 25 stages although typically only one or two are used Cycle A Cycle is the number of repetitions of a Stage Step A Step is a user defined program line containing a temperature a hold time and ramp rate Typically three steps are created within one Stage To enter the programming section of the Methods Cycler TCY software highlight methods ier iaiiieh wal Om og ne Up Arrow or Down Arrow keys CCIE snurre rom IA V ed to select
6. M V Press the Decimal Point key to insert a new step To insert a new step after an existing step use the Up Arrow or Down AO V keys to select the step Press the Decimal Point key to insert a new step Use the Up Arrow or Down Arrow keys to select the step you wish to delete Press Delete lt to delete the step The prompt line will flash between DEL STEP and YES or NO Press Enter dl to delete the step or Press Endy to return to editing the step 21 To add a new stage before an existing one Use the Up Arrow or Down Arrow Stage 1 keys to highlight the stage number V Number cycles Step 94 090 00m30 max Press Enter 4 to insert stage A new stage will be added before the to delete stage existing stage The other stage numbers will be adjusted as appropriate The new stage can now be edited as described above To add a new stage at the end Use the Up Arrow or Down Arrow V keys to highlight the dotted line below the Roset run 12 00 Sil last stage Bos lao lel LOTO C l P Enter to insert stage a A new stage will be added with the next consecutive number The new stage can now be edited as described above To delete a stage Use the Up Arrow or Down Arrow V er Stage 1 keys to highlight the stage you wish to Number cycles 10 delete Step 94 0 C 00m30 max gl to insert
7. N tuba a E vale aaa 11 TREK eee 12 6 Programming the Cycler TOY Lusssseasssnugnandandetomtdnmamlmisniue 13 Programming APS aa 15 Option 1 Creating programs USING templates rrrrrrrrnnrrnnrrrvnrrnrnnrrrnnrrnnnrrrnnrennnrennnrennnnennnsrennennnn 19 Option 2 Creating a new PrOQFaMcs cavesscawsecctasatanendusees ed sencuecenagdy eee 20 EXAMPIE DIOGFAINGS Sarre 20 PN 20 To program increment or decrement temperature or time rrrnrrnnrnanrnnnrnnnnnnnnnrvnnrnnrnnrnnernnennnener 24 Tornado EN sea peers 27 THOM ANN ua 29 8 COMMGUNAUOM sisie r aie hsdpa eee 29 9 Notes on transferring protocols from other thermal cyclers 0 000 32 10 USEF maintenan sicesettecscdedansuerseaesnsicariastensnenciausadenabeiumucutunetteaehesatamndnedeetbeteseusns 33 Cleaning ou CY CICK TOY rrine creer ht ae ae acc ead ace ae A 33 Heater BIOCK FEIMOVAl rdaardsscctant seat taneckas alas A danker naienk hidden 33 11 Some frequently asked questions r nnrnnnnnnnennnnnnnnnnnnnnnnnnnnennnennnnnnnnennnennnennnunnn 34 12 Additional information rennnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnennnnnnnnnnnnnnnnnnnnnnnnnnnnennnnnennnnnnnnnne 35 FEI 35 FUSES ard 35 The heated lid over temperature CUt OUt rrnrennnnnnnrnnnvnnrnnnrnnnnnnrnnnrnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnennuennne 35 HIS UI ACIOM USS UNG ae Ne hadde 35 INGEICNANGSADIC DIOCKS siaou tai veachaie ls Pere ar R TAE E A Nae 35 1 Introduction The Cycler TCY provides the researc
8. Press Enter This screen lists all the programs saved on New Methods the unit Use the Up Arrow and Down 2 Slee el Arrow keys NY to scroll through the list 3 STEP J Ay Ay co o The number next to the program name is the unique Speed dial number allocated to the program for rapid retrieval see below 14 Programming Options There are two options available for creating programs on the CYCLER 1 Copy and edit one of the pre written templates 2 Create a new program from the defaults Option 1 Creating programs using templates There are two pre written basic PCR templates a two step and a three step template These can be copied and modified as required In denat stands for Initial Denaturing 2 STEP 3 STEP Heated Lid LS Heated Lid iOS Piceimeeie ale on Premeat Lad on Pause Ofi Pause OLE In denat G OLE Sa 00 In denat GA ORSC SG HOW mele Off Hot Svare EE NN EN ne Ar Number cycles 20 Number cycles S Seep 9420 ESN ee NN ASP Step SO On Seo NN SOE NE EE se NN Poser ud I2507 C smo SoS Po aano Ead TOFO PBosr dun 12 O pos te Inollel OE Other templates available include Ice bucket incubation at 4 C Ligation incubation at 15 C and an RT PCR template Lanen Use the Down Arrow key V to select an EN existing Template program from the 2 STEP 2 17 menu e g 2 Step Template or 3 Step AV d d to select Press the Enter key lt l to
9. When you have finished press the Enter key to go back to the Configuration menug The Passwords function allows the administrator to change the Admin password which allows access to Configuration and also to delete any passwords associated with programs Highlight Passwords then press Ente The screen will appear as opposite We suggest that you change the Admin password at this stage in order to protect the configuration settings Use the Up Arrow key to highlight Admin passwd Press Enter lt and type in the new password Repeat to confirm and accept The screen will go back to the Configuration menu To delete a password associated with a program use the Up Arrow and Down ArrowN v keys to highlight the program Press Enter lt Press Enter lt l again to delete the password The Format memory will delete all saved programs from the unit Highlight Format memory then press lt Enter To delete all the programs press Enter Set ADMIN PASSWORD to 1234 31 9 Notes on transferring protocols from other thermal cyclers The CYCLER TCY heats at over 3 6 C s and provides the highest levels of temperature uniformity Many slower machines have historically begun the countdown on hold times before the set temperature is reached in order to avoid any overshooting in sample temperature The slower the heating
10. damaged as a result of excessive corrosion or current heat moisture or vibration improper specification misapplication misuse or other operating conditions outside of OMEGAS control Components in which wear is not warranted include but are not limited to contact points fuses and triacs OMEGA is pleased to offer suggestions on the use of its various products However OMEGA neither assumes responsibility for any omissions or errors nor assumes liability for any damages that result from the use of its products in accordance with information provided by OMEGA either verbal or written OMEGA warrants only that the parts manufactured by the company will be as specified and free of defects OMEGA MAKES NO OTHER WARRANTIES OR REPRESENTATIONS OF ANY KIND WHATSOEVER EXPRESSED OR IMPLIED EXCEPT THAT OF TITLE AND ALL IMPLIED WARRANTIES INCLUDING ANY WARRANTY OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE HEREBY DISCLAIMED LIMITATION OF LIABILITY The remedies of purchaser set forth herein are exclusive and the total liability of OMEGA with respect to this order whether based on contract warranty negligence indemnification strict liability or otherwise shall not exceed the purchase price of the component upon which liability is based In no event shall OMEGA be liable for consequential incidental or special damages CONDITIONS Equipment sold by OMEGA is not intended to be used nor shall it be used 1 as a Basic Component
11. external cause such as a faulty plug or lead Check both fuses and replace the faulty fuse with a new one of the correct value fuse values are given on the label next to the power inlet Note that fuses should only be replaced by a qualified electrician The holder for the two fuses is built into the power input socket First remove the power cable and then gently prise the fuse drawer open with a flat bladed screwdriver or similar tool Each fuse can be removed by using the screwdriver as a lever Exchange the faulty fuse in the fuse holder for a working fuse of the correct value Finally replace the fuse drawer in the fuse compartment and push the drawer shut Fuses which blow repeatedly indicate a serious fault and you should return the unit to your supplier for repair The heated lid over temperature cut out The heated lid is fitted with an independent circuit to protect it from overheating In the unlikely event of an over temperature problem with the lid the unit is fitted with a thermal fuse which will remove power to the heater plate should the maximum temperature be exceeded Insulation testing This equipment is fitted with RFI suppression circuitry Any check of the electrical insulation by means of high voltage dielectric testing for example as in BS EN 61010 1 must be carried out using only a TCY voltage This unit contains semiconductor components which may be damaged by electric field effects Interchangeable blocks The block
12. over the number of cycles in the stage For example if the 25 first temperature is 65 C and the last temperature is 55 C over 30 cycles the temperature will decrease 0 33 C each cycle Similarly in the example shown the time would increase from 30 seconds to 1 minute 30 seconds in 2 second increments To save the program program can only be run once it has Stage 1 been saved Press the V End key Number cycles Step 94 0 C 00m30 The prompt line will flash between SAVE SAVE and YES or NO d Yes or V No Press Enteral for Yes to save the changes you have made If you press End for No then all the changes you have made will be lost GEORGE l l The program is now ready to run and will Run method be allocated with a unique Speed dial View method number which can be used to rapidly locate the program from the main start up NY lt TOE eE screen Use the Up Arrow and Down Arrow NY keys to scroll through the list of options Stopping what you are doing At any time during programming the CYCLER when you are satisfied with the program you can finish and accept what you have done by first pressing the End key V Then follow the instructions on screen If you are not sure wait for the instructions in the prompt line to scroll back to the first line that you see 26 To run a program Methods ine ormation CO ine O eve OM
13. rate the more significant the loss in hold time i e if a 30 second hold time is set at 55 C then perhaps 5 to 10s will be lost in between the start of the time countdown and actually reaching the set temperature The overall effect of this method of control is that your sample if cooling from a higher temperature is subjected to an environment with an elevated temperature compared to the target temperature for a significant part of the hold time The CYCLER TCY provides the user with what you program is what you get Due to the rapid and responsive nature of the Peltier heating system the time between the timer countdown initiating and actually reaching the set temperature has been reduced to as little as 1s This ensures that the sample is only subjected to the exact temperature required Taking the above into consideration it must therefore be noted that care must be taken when re optimizing protocols from a slower machine Trouble Shooting 1 The optimum annealing temperature may in fact be higher than the set temperature used in the slower machine Try increasing it by 1 or 2 C 2 The hold time can be reduced once the optimum temperature has been found Try reducing it by 5 to 10s 32 10 User maintenance Cleaning your CYCLER The heating cooling block including wells and flat surfaces should be cleaned regularly to ensure optimum heat transfer to the samples Always clean the block if there has been a spillage Use a clot
14. some dummy tubes if necessary Switching on When you switch on the unit a screen TCY similar to the one shown here will appear Omega Engineering briefly This indicates the software version XX and block type installed in the unit Verson x Block Type 48x0 2ml When the initial screen has disappeared the screen shown here will automatically appear There are three options 1 Programs for creating and running Methods programs Information 2 Information for obtaining information Come Mace her about the unit e g serial number 3 Configuration for setting up program defaults and settings Each of these is explained in detail in section 6 IA V d to select 10 5 Front panel controls CE OMEGA Operation or Program lines rompt line Methods Information Configuration 4 Y d to select Alpha Numeric keys Status ro VEE Decimal Point 498 Insert Power A Enter Pause The LCD Display The CYCLER TCY has a four line LCD Methods 0 display The top three lines are Information information or program lines while the COn Ligur Tion bottom line is a prompt line The active line flashes on screen and is shown in ma NY 7 lt RO Selle Gur bold in this manual The bottom line may change or flash between two or three different prompts Number cycles 10 depending upon which program line is Step 94 00 00m30 active An exampl
15. stage Press Delete lt to delete stage The prompt line will flash between DEL STAGE and YES or NO Number cycles step 94 0 C 00m30 E Press Enter to delete the stage DEL STAGE a Yes or V No 22 To insert a pause into a program To insert a pause after an existing stage Step 34 0 C 00m30 max follow the steps for inserting a stage as 555 Stage 2 above Number cycles E Step 94 0 C 00m30 max Highlight the Number cycles line then press Pause to insert step AY gl to edit The prompt line will flash between Step 94 0 C 00m30 CHANGE TO PAUSE and YES or NO Number cycles Press Enter to insert a pause step 94 0 C O0Om30 max CHANGE TO PAUSE a Yes or W No The stage number will then be highlighted with Pause as the only line to delete step 23 To program increment or decrement temperature or time Under normal circumstances the Hold Temperature of all steps is constant However it is possible to automatically increment or decrement the temperature of a specified step of a programme 3 fl 0 400 io 0 300 3 0 200 2 0 30 m 3 O e A oi Lee ee es EE EEE eee eee ESTE Q E 6b 2 2 2 2 2 Cycle 1 Cycle 2 Cycle 3 Cycle 4 Cycle 5 Temperature profile of incremented Hold Temperature at step 3 of each cycle The Hold Temperature of the incremented dec
16. the changes you have made will be lost The program is now ready to run and will be allocated with a unique Speed dial number which can be used to rapidly locate the program from the main start up screen Up Arrow or Down Arrow keys Vv to scroll through the remaining list of options 16 To edit a field or step in an existing program When the unit is switched on the display Methods will show this screen TATOM e oN Cone eoo e ateake nn If known type in the Speed dial number for the required program and press AN Y lt to select Enter This will take you directly to the required program If you do not know the Speed dial DAVID number press Enter lt to access the DONNA list of stored programs Either yp dhe diss select 1 Scroll to the required program using the Up Arrow or Down Arrow keys or NY 2 Type the first letter of the program name in the New program field this will then list all the program names beginning with that letter Use the Up Arrow or Down Arrow keys NY to select the required program Press Enter to accep The full set of options for a program is DONNA shown here these are shown on the LCD Run method screen three lines at a time View method Use the Down Arrow and or the Up HOLL NS ere el Arrow keys to Copy method select the option you require Delete method Press
17. tube or reaction vessel other than those described in this manual We recommend using reaction volumes between 20 and 200ul The tubes must withstand a pressure of 1 atmosphere at 100 C Any vessel must be able to withstand the temperatures you are using without any danger of them deforming to the point where they fracture To test your tubes put 25ul of water into each of 5 tubes and subject them to a typical thermal cycling protocol At the end of the program measure the volume remaining using a micropipette A loss of more than 1 or 2ul indicates a vapour leak The amount of volume loss you observe and the change in reactant concentrations you can tolerate determine the minimum volume that can be used Typical volume losses of 1pul in 30 cycles allow the use of samples of 20ul or less During the final cool down a ring of condensation may form above the liquid level but below the top of the sample block This is normal and does not effect the reaction 0 5ml Tubes The heated lid mechanism of the Cycler TCY with the 0 5ml block is optimised for flat top tubes In order to fit the maximum number of tubes in the block they need to be placed so that all the hinges are in the same relative orientation An example is illustrated below Place the tubes in the block in the same orientation in order to achieve the maximum capacity Always make sure the pressure on the heated lid is balanced by spreading the tubes across the block or inserting
18. IA V ed to select Methods ks OANE L O CCIE ora eN AV ed to select x New Methods 2 STEP DET 3 STEP 34 IN V pd to select DAVID DONNA g to select DONNA Run method View method AV d to select RUN TMETHOD 7 Yes or V No When the unit is switched on the display will show this screen If known type in the Speed dial number for the required program and press lt Enter This will take you directly to the required program If you do not know the Speed dial number press Enter p to access the list of stored programs Next either 1 Scroll down to the required program using the Up Arrow and Down Arro keys 2 Or type the first letter of the program name in the New program field this will then list all the program names beginning with that letter Use the Up Arrow and Down Arrow keys to select the required program Press Enter to accept lt For example Donna To run the program select Run program and press Enter A screen similar to this one will appear Press Enter to start the run 21 sole 20 56m08 sample S C S57 7S Holes 00m17 Vor Ster I to PAUSE lt for info Manual pause Celo 740 80 19m26 Sample 72 0 C ES RKO IC IG Fe 00m10 PAUSET YES or V NO Cycle 24 30 19m26 Sample 72 0 C Ser Paused Wo TOEP to CONTINUE lt for info Man
19. V d to select 1 Blod 19965 48x0 2ml Blockin 1234560789 Uma Sia GN SA SG S w version om H w version DE Comms address il Unit cycles 624 Blo kE S oe oo 8 Configuration Pressing Enter while a program is running will give information about the current run including which program is actually running and the unit status e g holding or ramping An example is shown here The Information field on the front screen contains all the unit specific information such as serial number and software version Press Enteral to access the unit information The complete list is displayed here Warning The Configuration software should only be accessed by designated personnel as some parameters can be changed or may be lost when using it Methods linet Osea abe mi Configuration IA V ed to select The Configuration field on the front screen allows a system administrator access to set up all the default settings for the programs time and date settings and to change and remove passwords 29 Access to the Configuration set up is CONFIGURATION password protected Default admin passwords is 1234 Password 123481 To enter configuration use the alpha keys amp d to accept to type in 1234 V to exit Press Enter SSL There are a number of options which can Lile be changed these are listed here Passwords Format MEMO Use the Up Arrow and Down Arrow NY keys to
20. Yes 100 C to 115 C Fixed at 145 C Thermistor Etched foil 33W 4 min approximately ambient to 105 C The heated lid will only come on if the set temperature is above 35 C Programming Program naming using alpha keys Program password protection Number of programs 3 step Maximum number of steps per stage Maximum number of stages per program Minimum hold time Maximum hold time Incremental decremental temperature Incremental decremental step hold time Programmable ramp rate heat or cool Programmable ramp rate resolution Yes Yes 37 35 25 1 sec 18 hrs Yes Yes Variable in 0 1 C sec steps 0 1 C Running Programs Pause button Stop button End of program alarm Auto restart on power fail Yes Yes Yes Yes Interconnectivit Connection to PC control program Software updates from web site Yes Yes Dimensions Height 185mm Width 185mm Length 330mm Power Power consumption 230W Units marked 100V will work at any voltage between 90 and 110V The performance will vary and will not necessarily meet the above typical specification at the extremes of voltage 4 User operation and consumables Heated lid To release the heated lid lift the lid catch and the lid will open To close the lid push down gently until the catch clicks in place Do not close the lid without tubes being fitted in the block Tubes or reaction vessels Omega Engineering Inc does not recommend any specific
21. accept it The Template is already defined and can be run as it is or easily copied modified 2 STEP Run Method and saved under a new name View Method AV to select 15 Heated Lid 105 Preheat Lid on to edit To give a program a name p UNNAMED Heated Lid 105 C Preheat Lid on t d to edit Heated Lid 105 C Preheat Lid on to edit V to save To save the program DONNA Heated Lid Preheat Lid SAVE I el YES or V NO 7 DONNA Run Program View Program T to select A new name can now be given to the copied program see below To type in a name for the program press Enter anda cursor will appear in the position shown Using the keypad type in the name you wish to give the program The letters will scroll round with successive presses for example the 2 key will give A with one press B with two presses C with three presses and 2 with four presses Five presses will again give A When the name is complete press lt Enter There are now two options 1 Save the program and run it as it is or 2 Editthe program then save and run it A program can only be run once it has been saved Press the End V key The prompt line will flash between SAVE and YES or NO Press Enter for Yes to save the changes you have made If you press End for No then all
22. am or End to return to the program screen If you want to password protect the program so that other users cannot edit it select Set password A screen similar to this will appear Type in the password you wish to give it and press Enter lt The program list will then display the key symbol next to the name to show that it is locked Other users will still be able to copy view your program but not edit it A second option is to create a completely new program without using templates UNNAMED Heated lid TOO E Preheat lid OET Pause GE IE In denat oA 0 amv IONE STEGET OPTIE 12 0 C MOO Ore Post run Post hold A number of typical values are pre entered including Heated lid temperature Preheat lid Initial denaturation Hot start Final extension Final hold These values can be edited and the additional stages and steps required for cycling can be added by following the instructions detailed on the following pages 19 Example programs A couple of example thermal cycling programs are shown below Typical 3 Step PCR 3 Step PCR with multiple Stages Heated Lid Preheat Lid Heated Lid Preheat Lid In denat HOL bare HOC Starr Stage I Number Cycles Number Cycles Step gaie step 94 c Step 55 eo step 60 c WO Stage 2 Post hold Number Cycles step 94 c step 55 Cc Post hold Programming Create a new program by selecting New New
23. can be removed and replaced by another of the same sort or of a different sort The software will register the change and set up the unit for the new block Remove the four screws on the underside of the unit and drop the block fan assembly down Unplug the assembly from the wiring and remove the assembly from the unit Refitting the block fan assembly is the reverse procedure be careful of the wires as you refit the block 35 WARRANTY DISCLAIMER OMEGA ENGINEERING INC warrants this unit to be free of defects in materials and workmanship for a period of 36 months from date of purchase OMEGA s WARRANTY adds an additional one 1 month grace period to normal three 3 year product warranty to cover handling and shipping time This ensures that OMEGAS customers receive maximum coverage on each product If the unit malfunctions it must be returned to the factory for evaluation OMEGA s Customer Service Department will issue an Authorized Return AR number immediately upon phone or written request Upon examination by OMEGA if the unit is found to be defective it will be repaired or replaced at no charge OMEGA s WARRANTY does not apply to defects resulting from any action of the purchaser including but not limited to mishandling improper interfacing operation outside of design limits improper repair or unauthorized modification This WARRANTY is VOID if the unit shows evidence of having been tampered with or shows evidence of having been
24. decrement times and temperatures It is also used to pause a program when it is running The Enter or Accept key The Enter key is used to enter an operation or the edit mode It is also used to Accept a field or program change The End Finish or Exit key The End or Finish key is used to finish editing programs or to exit a program It is also used to exit a sequence of key operations The Up arrow key The Up arrow key is used for scrolling up through the lines on the screen It is also used to increase certain fields when editing 12 The Down arrow key The Down arrow key is used for scrolling down through the lines on the screen It is also used to decrease certain fields when editing The Decimal Point and Insert key The Decimal Point key is used when editing numeric fields It is also used as an Insert key during program editing to insert additional steps and other field sets The Delete key The Delete key is used during program editing to delete steps and stages The Pause key The Pause key is used during program editing to insert a pause after a stage or to insert increment and decrement times and temperatures It is also used to pause a program when it is running The Enter or Accept key The Enter key is used to enter an operation or the edit mode It is also used to Accept a field or program change The
25. e of a prompt line which fo 1 0 ap t at flashes between three different prompts is i ees EE shown here TL to edit to delete step Indicators O Power This indicator is lit when there is power to the CYCLER TCY Pai Status This indicator is lit when the CYCLER TCY is running a program It is O off when the unit is stopped It will flash and beep slowly when the unit is 2 paused It will flash after a five second quick sequence of beeps when the unit reaches its final hold temperature 11 The keys The End Finish or Exit key The End or Finish key is used to finish editing programs or to exit a program It is also used to exit a sequence of key operations The Up arrow key The Up arrow key is used for scrolling up through the lines on the screen It is also used to increase certain fields when editing The Down arrow key The Down arrow key is used for scrolling down through the lines on the screen It is also used to decrease certain fields when editing The Decimal Point and Insert key The Decimal Point key is used when editing numeric fields It is also used as an Insert key during program editing to insert additional steps and other field sets The Delete key The Delete key is used during program editing to delete steps and stages The Pause key The Pause key is used during program editing to insert a pause after a stage or to insert increment and
26. either the time or temperature incrementally over the number of cycles in a stage Incrementation of extension time is used with Long PCR which is when large template fragments are to be amplified e g 27kb lambda DNA 40kb ge nomic DNA Decremental temperature is used for protocols such as Touchdown PCR where one starts with a high annealing temperature in the first cycle and gradually decreases the temperature over the number of cycles inthe stage This ensures that only the specific product is amplified The heat up ramp rate in the specification is 3 6 C second yet I can only manually set it to 2 0 C second Why is this If you set the ramp rate to max the block will heat at 3 6 C second the unit sets its own control parameters If you program a ramp rate the control parameters reduce the ramp rate This is a feature of the software and not the actual ramp rate 34 12 Additional information Brief fault finding notes and a list of replacement parts are given in this section Note that this equipment should only be dismantled by properly trained personnel Removing the outer cover exposes potentially lethal power voltages There are no user serviceable parts within this equipment Fault Finding Should you encounter problems with your TCY contact Omega Flow Engineering for assistance Fuses If neither the power light nor display on the front panel is lit one of the two fuses may have blown Check that there is no
27. erial number of the product and warranty and 3 Repair instructions and or specific problems 3 Repair instructions and or specific problems relative to the product relative to the product OMEGA s policy is to make running changes not model changes whenever an improvement is possible This affords our customers the latest in technology and engineering OMEGA is a registered trademark of OMEGA ENGINEERING INC Copyright 2009 OMEGA ENGINEERING INC All rights reserved This document may not be copied photocopied reproduced translated or reduced to any electronic medium or machine readable form in whole or in part without the prior written consent of OMEGA ENGINEERING INC Where Do I Find Everything I Need for Process Measurement and Control OMEGA Of Course Shop online at omega com TEMPERATURE lA Thermocouple RTD amp Thermistor Probes Connectors Panels amp Assemblies lA Wire Thermocouple RTD amp Thermistor ut Calibrators amp Ice Point References WA Recorders Controllers amp Process Monitors lt Infrared Pyrometers PRESSURE STRAIN AND FORCE lA Transducers amp Strain Gages WA Load Cells amp Pressure Gages lt Displacement Transducers lA Instrumentation amp Accessories FLOW LEVEL l Rotameters Gas Mass Flowmeters amp Flow Computers bt Air Velocity Indicators WA Turbine Paddlewheel Systems lA Totalizers amp Batch Controllers pH CONDUCTIVITY LA pH Electrodes Testers am
28. h or cotton buds dipped in a fresh 50 50 water isopropanol solution and make sure that no deposits are left in the wells In the case of radioactive spillages the heater block can be removed from the unit for more detailed cleaning Omega Engineering Inc recommend that you use a proprietary cleaning agent and follow the manufacturer s instructions The heating cooling block is made of aluminum therefore an agent such as Neutracon from Decon Labs Inc suitable for nonferrous metals should be used However remember that other parts of the unit are made of ferrous materials and may be damaged by spillage onto them Heater Block removal Before cleaning your unit disconnect from the power supply and allow it to cool to below 50 C Remove the four screws on the underside of the unit and drop the block fan assembly down Unplug the assembly from the wiring and remove the assembly from the unit Refitting the block fan assembly is the reverse procedure be careful of the wires as you refit the block iy 4 f Cc a oo j J LT LT Cc Cc ET co Boe NG Underside of the CYCLER TCY Four screws which hold the block fan assembly in place The outer case of the CYCLER TCY can be cleaned with a cloth dipped in water or ethanol methanol or formaldehyde can also be used No part of the case or cover should be immersed in the solvents Do not use aggressive solvents such as acetone or abrasive cleaners 33 11 Some frequent
29. her with the means of accurately controlling the temperature profile of samples It has many scientific applications including DNA amplification and sequencing The Cycler TCY can cycle samples between 4 C and 99 C The Cycler TCY is programmed by means of an integral keypad and LCD display A program which can be recalled from memory consists of e aseries of specified temperatures in C e the times for which each specified temperature will be held Hold Times e the desired heating or cooling rates in C sec between each specified temperature e optional instructions as to whether the times and or the temperatures are to increase or decrease when a cycle is repeated The memory can store up to 80 programs Cycler TCY is the most economic personal thermal cycler There are four types of blocks can be chosen for this model 0 2ml X 25 and 0 5ml X 20 wells blocks which have less sample compartment but with higher ramping rate 0 2 ml X 48 and 0 5ml X 60 wells blocks which have more wells but less ramping rate Before using the TCY units make sure you have read this manual carefully If there is any doubt relating to the proper use of this equipment contact Omega Flow Engineering for assistance 2 Safety and installation Operator Safety Please read this manual carefully before using the thermal cycler If the equipment is not used in the manner described in this manual the protection provided by the equipment might be impaired Al
30. l Omega Engineering Inc instruments are designed to conform to international safety requirements and are fitted with an over temperature cut out If a safety problem should be encountered then switch off the unit at the power source and remove the plug from the electricity supply Warning HIGH TEMPERATURES ARE DANGEROUS as they can cause serious burns to operators and ignite combustible material Users should be aware of the following potential hazards e USE CARE AND WEAR PROTECTIVE GLOVES TO PROTECT HANDS e DO NOT use combustible substances near hot objects e DO NOT operate the instrument in the vicinity of inflammable liquids or gases e DONOT place any liquid directly into the instrument always use the appropriate plastic consumables After heating samples remember that parts of the instrument namely the lid and block and any associated accessories may be very hot Unpacking When unpacking please ensure that the following have been removed from the packaging e Thermal cycler e Power cable The user is advised to keep the original packaging in case the instrument ever needs to be returned for service or repair Omega Engineering Inc accepts no responsibility for damage incurred unless the unit is correctly packed and transported in its original packaging Please note that the screen of the thermal cycler is easily damaged by sharp objects such as pens pencils and fingernails This type of damage will be considered as misu
31. ly asked questions Q1 A1 Q2 A2 Q3 A3 Q4 A4 Q5 A5 What is the Pause function at the start of the program used for Some users prefer to preheat the heated lid before placing the samples into the unit The pause feature is used to stop the unit after the 4 minute heated lid preheat step It will also sound an audible alarm indicating that the machine is ready forthe sample tubes or plate to be added Pressing the Pause key followed by the Enter key will commence the remaining program Why do you have to press some keys twice to make the unit do what you want it to do With many of the program features there is a fail safe function which asks if you are sure you want to perform a particular action You are required to press one key to select a function followed by a second to accept it What is Hot Start A hot start is used for reducing non specific product formation during the first cycle of amplification The Hot S tart programming step is used to pause the instrument at a specific temperature typically around 70 C after the initial template denaturation The reason is to allow the manual addition of unmodified Taq DNA polymerase which may loose activity if added during the initial 5min denaturation Heat activated Taq or Hot Start enzy mes do not require this step What is the incremental function See page 24 Incremental timing and temperature are used to increase or decrease
32. nutes 1 For whole minutes type the number required then Enter lt 2 To toggle between minutes and hours use the Decimal Point key 3 For values less than 1 minute first press the Decimal Point key followed by the number of seconds required 4 For minutes and seconds type the number of minutes followed by the Decimal Point key then the number of seconds Once the values have been added press Enter to accept Once you have finished editing and you are happy with the changes press the End key WV followed by the Enter key to save If you press the End key V a second time for No then all the changes you have made will be lost 18 To copy a selected program UNNAMED Heated Lid 105 C Preheat Lid on TM to edit to save To delete a selected program To delete a selected program DONNA delete program SURE amp Yes or W No To set a password for a selected program DONNA New passwd E l to accept V to exit Option 2 Creating a new program If you select Copy program a screen similar to this will appear and the software will automatically give the program the next number until it is named Press Enter and type in a new name You will then be able to edit the program as previously described If you select Delete program a screen similar to this will appear Press Enter to delete the progr
33. p Accessories WA Benchtop Laboratory Meters l Controllers Calibrators Simulators amp Pumps lt Industrial pH amp Conductivity Equipment DATA ACQUISITION lA Data Acquisition amp Engineering Software WA Communications Based Acquisition Systems WA Plug in Cards for Apple IBM amp Compatibles lt Datalogging Systems WA Recorders Printers amp Plotters HEATERS l Heating Cable l Cartridge amp Strip Heaters ut Immersion amp Band Heaters WA Flexible Heaters lt Laboratory Heaters ENVIRONMENTAL MONITORING AND CONTROL WA Metering amp Control Instrumentation lA Refractometers lA Pumps amp Tubing lA Air Soil amp Water Monitors lA Industrial Water amp Wastewater Treatment l pH Conductivity amp Dissolved Oxygen Instruments M 5380 0114
34. re being used to permit efficient air flow for each instrument 6 Switch on the instrument Power Switch On O Power Switch Off Replacement cable Should the power lead need replacement a cable of 1mm of harmonized code HOSVV F connected to an IEC320 plug should be used IF IN DOUBT CONSULTA QUALIFIED ELECTRICIAN Working Conditions The thermal cycler is designed operate under the following conditions Indoor use Ambient temperature range 5 C to 40 C Altitude to 2000m Relative humidity not exceeding 95 Power supply fluctuations not exceeding 10 Over voltage category II IEC 60364 4 443 Pollution degree 2 Note The control specifications are quoted at an ambient temperature of 20 C The specification may deteriorate outside an ambient temperature of 10 C to 30 C The instrument has been tested for radio frequency interference and is certified under EN61326 3 Cycler TCY Specification Temperature Temperature range Temperature set point precision Block uniformity over full range Temperature accuracy End of program cooling below ambient 4 C to 99 C 0 1 C 0 5 C 0 5 C Yes Heating Cooling Rate Heated Lid Maximum heating rate Minimum heating rate Maximum cooling rate Minimum cooling rate Heated lid enable disable Selectable heated lid temperature Over temperature cut out Temperature sensor Heater type Heater power Warm up time 4 5 C s 0 1 C s 3 0 C s 0 1 C s
35. remented step is the defined Hold Temperature plus the summation of the increments decrements The first cycle is never incremented decremented only subsequent cycles If you select a decrement the Hold Temperature is prevented from falling below 4 C If you select an increment the Hold Temperature is prevented from rising above 99 C Similarly under normal circumstances the Hold Time of all steps is constant However it is possible to automatically increment or decrement the duration of a specified step of a program Temperature t t t 20 t 30t t 4ot Cycle 1 Cycle 2 Cycle 3 Cycle 4 Cycle 5 Temperature profile of incremented Hold Time at step 3 of each cycle The Hold Time of the incremented decremented step is the defined Hold Time plus the summation of the increments decrements The first cycle is never incremented decremented only subsequent cycles If you select a decrement the Hold Time is prevented from falling below one second Use the Up Arrow or Down Arrow Number cycles keys to move to the step where you want Step 94 0 C 00m30 an increment or decrement temperature or Step 58 0 C 00m30 max time to edit i Press Enter to go into edit mode amp to delete step to insert step Press Pause LI 24 Number cycles Step 94 0 C 00m30 Step 58 0 C 00m30 CHANGE STEP I d Yes or V No Number cycles step 94 0 C 00m30 fst 55 0 C 00m30 Tr amp to edi
36. se and invalidate the guarantee for this component Installation The instrument should be carried using both hands Never move or carry the instrument when in use or connected to the power electricity supply 1 All Omega Engineering Inc instruments are supplied with a power cable this may be integral or plug in 2 Before connecting the instrument to the power electricity supply check the voltage against the rating plate located on the back of the unit Ensure that the voltage selector switch located above the On Off switch is set to the appropriate voltage for the local supply Please note that the unit must be grounded to ensure proper electrical safety Connect the power cable to a suitable plug according to the table below 220 240V 50 60Hz Supply 110V 120V Supply 50 60Hz 3 Units showing 230V 50 60Hz on the rating plate also operate between 210 and 260V units with 120V 50 60Hz also operate between 100 and 130V and units marked with 100V will operate between 90 and 110V In all cases the heating rate will degrade by approximately 8 at the extremes of the voltage range Plug the power cable into the socket on the back of the instrument Place the unit on a suitable flat bench or in a fume cupboard if required ensuring that the air inlet vents on the underside are free from obstruction Position the instrument with a minimum distance all round of 200mm from walls or other items and between each unit if multiple units a
37. select then press Enter lt to Se Libel ll oie ce access any of the options Each option is discussed in detail below Settings Settings Highlight Settings then press Enter Passwords Format memory Self calibrate Highlight Settings then press Enter Settings oe lt Passwords Hormeasne nor y Self Calibrate Ger dare XX XX XX The complete list of settings is given here Set time XX XX Auto restart on will allow the unit to Auto re Cart on restart from where it left off should there be a power failure and the power returns BEGJEOS KE on Come addr ocs 1 The Comms address refers to the location of a unit in a daisy chain and Come es pE a ee o must be unique if there are several units linked to each other IA V sd to select The optimum Coms speed is set at 19200 When using a control software ensure all cyclers are set to the same value Edit any line as described for Program defaults above Press Enter lt to accept the new value 30 Passwords Settings Passwords Format Memory Self calibrate Admin passwd DONNA GEORGE Ty to select ADMIN PASSWD Password a Confirm amp to accept Admin passwd DONNA GEORGE DEL PASSWORD amp d Yes or W No Controller reset Settings Passwords Format memory Ssellfsealhbr are Format Memory This will delete all methods DURE lt e YES V NO
38. t V to save Number cycles 30 Step 94 092 00m3 0 fet BE 65 C 00m30 max edit temperature Step 94 0 C 00m30 fst 65 0 C 00m30 let 55 0 C 00m30 a to edit amp to delete step to insert step 94 0 C O00m30 max 65 0 C O0ma0 max 55 0 c 1m30l edit time For hrs mins The prompt line will change to flash between CHANGE STEP and YES or NO Press Enter The cursor will again move into the edit mode Now press the Up Arrow key The step will change to fst meaning first and the software will insert another line after it entitled Ist meaning last Press Enter twice to go into edit mode Use the Alpha numeric keys to change the values of the fst line to the first temperatures and or times you require Press Enter after each field you change You will exit Edit Mode when you reach the end of the line Use the Down Arrow key y to move onto the Ist line Press Enter to edit the step Use the Alpha numeric keys to change the values of the 1st line to the final temperatures and or times you require Press Enter for each field you change You will exit Edit Mode when you reach the end of the line When you run the program it will automatically increment or decrement in equal steps from the first time and or temperature to the last
39. the Enter key lt to select the set password option AV d to select Edit a selected field or step DONNA Select Edit program and a screen similar Heated Lid 105 C to this will appear Preheat Lid on to edit Use the Down arrow and or the Up arrow keys Ny to select the field or V to save step you want to edit You can now edit the active field or step by ne pressing the Enter key 17 Heated Lid B1i0s c Preheat lid on edit temperature to disable DONNA Heated Lid LOS Preheat lid Bon T to change turn on off In denat 4 0 C 00m30 edit temperature to disable In denat 94 0 c 0m30 edit time Posi roa 12 O8G Sumo Post hold 10 C AY d to edit V to save To edit a temperature type in the required temperature In the example here the heated lid temperature is being edited Press Enter lt to accept Use the Down Arrow and or the Up Arrow keys y to select the next field or step you wish to edit To edit a field with on off settings first select the field and press Enter Use the Up Arrow and Down Arrow keys to toggle between the settings Press Enter lt to accept To edit a step with both a temperature and a time first press Enter lt and type in the new temperature Press Enter again Now type in the new time The default is mi
40. ual Stop Cycle 28 30 10m39 same NE 00m10 HOO FG Sova ENG AG STOP PROGRAM d YES or V NO During the run the LCD screen will report 1 The cycle number 2 The time remaining counting down in seconds 3 The sample temperature whether holding or ramping 4 Maximal ramp rate 5 The current step of the run 6 A count down of the hold time An example is shown here To pause a program while it is running press Pause j The run will continue but the prompt line will alternate PAUSE and YES or NO To confirm that you want the program to pause press Enter If you have pressed the Pause key by mistake and you want the program to continue press End V The unit will emit a beeping sound while paused To resume the run press Pause J followed by Enter lt to continue To stop a program while it is running press End V The run will continue but the prompt line will alternate STOP PROGRAM and YES or NO To confirm that you want to stop the program press Enter If you have pressed the End V key by mistake and you want the program to continue press End V again When a program is stopped the unit will emit a beeping sound and the original program screen will appear 28 Program Information DONNA Heated Lid on Status ramping to exit 7 Information Methods 0 Information Confiquratior I
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