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Olympus UIS2 User's Manual

1. MPLN5xBD MPLN10xBD MPLN20xBD 926 3 g 3 a Rg me s elg ss gl7 is by 623 6 Il Il S g 929 932 MPLN50xBD MPLN100xBD Unit mm hos Widefield eyepiece WHN10x UIS2 objective lenses Field Number 22 Objective lens Numerical Working distance Focal distance Weight Total Practical field of Depth of focus magnification Aperture mm f mm g magnifications view mm um MPLN 5xBD 0 10 12 0 36 137 50 4 4 98 MPLN 10xBD 0 25 6 5 18 155 100 2 2 18 MPLN 20xBD 0 40 1 3 9 162 200 11 6 1 MPLN 50xBD 0 75 0 38 3 6 157 500 0 44 1 4 MPLN 100xBD 0 90 0 21 1 8 160 1000 0 22 0 73 Screw W26x0 706 17 eee es ae ee a ES ede UIS OBJ ECTIVE LENSES M Plan Apochromat BD MPlanApo BD Highest class Plan Apochromat objective lens that maximize performance in brightfield and darkfield observations All aberrations are corrected at the highest level while providing high N A MPlanApo100xBD 926 4 5 45 Unit mm oye Widefield eyepiece WHN 10x Super widefield eyepiece SWH10x UISLODJECHVETEDSES Field Number 22 Field Number 26 5 Objective lens Numerical Working distance Focal distance Weight Total Practical field Depth of Total Practical field Depth of magnification Aperture mm f mm g magnifications of view mm focus um Imagnifications of view mm focus um MPlanApo100xBD 0 9 0
2. 124 17 47 stroke Specimen surface For installation dimensions refer to those for the BXFM F page 19 Weight 8 2kg exclude objective lens Unit mm 20 MICROSCOPE SYSTEM BXFM BXFM S BXFM F BXFM ILHS Compact focusing unit suitable for building into existing equipment Pillar axis Holder mounting position Light axis R i ica Revolving nosepiece mounting position a Bany Objective lens mounting position Weight 2 4k Unit mm y Specimen position 9 9 BXFM S combination sample BXFM F BXFM ILHS TR30 2 U KMAS U LH100L 3 OL 290 Specimen surface 84 208 For installation dimensions refer to those for the BXFM F page 19 Weight 5 5kg exclude objective lens Unit mm 21 ee eS ne ee ES MICROSCOPE SYSTEM BXFM Stands A wide variety of stands are available to suit different applications and purposes Z2 STU2 Universal stand type 2 ai le 214 435 Si a b n A n n iy i k a gt OLYMPUS
3. 4 94 5 68 C bore 5 Deep 4 94 5 613 C bore 5 Deep 240 Weight 6 5kg nit nit mm 42 Ss ees eT ee ee OPTICAL TERMINOLOGY 1 Field Number F N and Practical Field of View The field number F N is referred to as the diaphragm size of eyepiece in mm unit which defines the image area of specimen The diaphragm diameter actually seen through eyepiece is known as the practical field of view F 0 V which is determined by the formula z Eyepiece F N Objective lens magnification F O V mm 2 Working Distance W D The distance between the front edge of the objective lens and the specimen surface with the surface of the cover glass in case of the cover glass objective lens when the specimen is focused 3 Parfocal Distance It is the distance between the objective lens mounting plane and the specimen In UIS2 UIS objective lenses the parfocal distance is designed at 45mm Working distance and parfocal distance Objective lens mounting position F Parfocal distance Working Distance W D ee kba y Focal plane For parfocal distance of the LCPLFLN LCD series objective lenses refer to the appropriate objective lens page 4 Relationship between the objective lens s focal length and magnifications Indicated magnifications
4. WD 6 5 LMPlan10xIR lt 920 32 gt 44 45 26 5 23 WD 18 5 LMPlan100xIR 920 32 45 41 6 WD 3 4 40 8 36 8 14 5 45 lt 920 32 gt LMPlan20xIR 4 5 45 MPlan100xIR 920 32 aaa Unit mm UIS objective lenses Wide ield eyepiece WHN10x Field Number 22 Objective lens Numerical Working distance Focal distance Weight Total Practical field of Depth of focus magnification Aperture mm f mm g magnifications view mm um LMPlan 5xIR 0 10 20 0 36 73 50 4 4 98 LMPlan 10xIR 0 25 18 5 18 73 100 2 2 18 LMPlan 20xIR 0 40 8 1 9 110 200 1 1 6 1 LMPlan 50xIR 0 55 6 0 3 6 115 500 0 44 2 2 LMPlan 100xIR 0 80 3 4 1 8 122 1000 0 22 0 87 MPlan 100xIR 0 95 0 3 1 8 130 1000 0 22 0 67 Screw W20 32x0 706 0 8 x1 36 13 M Plan SemiApochromat BD UIS2 OBJ ECTIVE LENSES BD Brightfield Darkfield MPLFLN BD series Plan SemiApochromat objective lenses giving high level correction for chromatic aberration The series secures a W D of 1mm or longer Since the exit pupil position of the 5x 150x objective lenses is standardized the position of the DIC prism does not have to be switched when changing the magnification MPLFLN5SxBD MPLFLN50xBD MPLFLN10xBD MPLFLN
5. Weight 800g Weight 800g Weight 1kg Insert the DIC dummy when not using the DIC slider BD M AD Adapter to mount BF objectives W26x0 706 W20 32X0 706 Weight 10g Unit mm 35 E eee VIDEO CAMERA ADAPTERS C mount video camera ports Allows direct attachment of a C mount video camera Four types are provided 0 63x 0 5x 0 35x and 0 25x All models feature a focus adjustment function U TV0 25xC U TV0 35xC 2 C mount video port with 0 25x lens 17 53 17 53 Image plane 1 32UN x C mount video port with 0 35x lens Weight 1 2kg Weight 100g U TV0 5xC 3 U TV0 63xC C mount video port with 0 5x lens C mount video port with 0 63x lens 930 1 32UN Image plan 1 32UN j Image plane ajg 060 Weight 200g Weight 430g Unit mm Field of view F N SENE EEN i Video camera adapter Projection Projection area F N 2 3 CCD Projection lens magnifications 2 3 CCD 1 2 CCD 1 3 CCD 1 2 CCD U TV1x 2 1x 11 8 6 U TV0 63xC 0 63x 17 5 12 7 9 5 U TV0 5xC 3 0 5x 22 16 12 U TV0 35xC 2 0 35x 22 17 1 U TV0 25xC 0 25x 24 Practical field of view mm Projection area Projection area Field Number Objective lens magnifications Focus the video camera adapter to prevent defocusing the eyepiece image and defocusing by magnification switching Gene
6. 0 63x U TV0 5xC 3 0 5x U TV0 35xC 2 0 35x U TV0 25xC 0 25x Figure 2 Imaging device size Camera format Diagonal Horizontal Vertical 1 3 6 0mm 4 8mm 3 6mm 1 2 8 0mm 6 4mm 4 8mm 2 3 11 0mm 8 8mm 6 6mm The above table is for standard image device sizes Check your device size for precise calculation Figure 3 Imaging device size and monitor magnifications anera iomat Monitor size diagonal 9 12 14 21 27 1 3 38 1x 50 8x 59 2x 84 6x 114 1x 1 2 28 6x 38 1x 44 5x 63 5x 85 7x 2 3 20 8x 27 7x 32 3x 46 2x 62 3x Example What is total magnifications for video monitor when objective lens is 50x video camera adapter U TV0 5xC and 2 3 video camera are used ere a a es ay eee OPTICAL TERMINOLOGY eTotal magnification on the video monitor Mob 50x M video camera adapter is 0 5x from Figure 1 and monitor magnification is 46 2x from Figure 3 M monitor observation M obyxM video camera adaptermonitor magnification 50x0 5x46 2 1155x Practical filed of view for video observation horizontal side M ob 50x M video camera adapter is 0 5x from Figure 1 and horizontal side of 2 3 imaging device is 8 8mm from Figure 2 Practical field of view _ Image device size for video observation 8 8 mm 50x05 72524M M ob X M video camera adapter 6 Numerical Aperture N A The numerical aperture is a key factor t
7. 31 1 8 180 1000 0 22 0 59 1000 0 27 0 59 Screw W26x0 706 18 a a ee ee MICROSCOPE SYSTEM BXFM BXFM frame BXFM F Widely used system that allows use in combination with fiber illumination motorized revolving nosepiece and telan lens unit Can easily be integrated into other equipment Attach to the equipment by rear bolt mounting screw or pillar mounting hole 4 M4 depth9 36 4 M8 depth8 Bolt mount screw Stroke 23 lt 3 7 13 100 124 0 5 17 0 5 2 7 4 FA 62 4 M4 depth7 Pillar mount hole center 66 2 p N 17 98 110 Weight 1 9kg Unit mm 19 MICROSCOPE SYSTEM BXFM BXFM BXFM F BXFM ILH BXFM ILHSPU Accommodates the reflected light brightfield darkfield and fluorescence illuminators 180 RR Light axis 165 lt 130 Pillar axis 83 Holder mounting position Light axis Revolving nosepiece mounting position g gt Objective lens mounting position Stroke 23 lt 3 7 a Specimen position Weight 3 2kg Unit mm BXFM combination sample BXFM F BXFM ILH BXFM ILHSPU TR30 2 B X RLA2 U LH100L 3
8. Metallurgical no cover Long working distance metallurgical use Super long working distance metallurgical use Observation through substrate Infinity corrected optical system Cover glass thickness no cover PL Plan None Achromat Corrects field Corrects aberration at 2 wavelengths of curvature of blue and red the periphery of FL SemiApochromat the image plane Corrects chromatic aberration in the visible range violet red APO Apochromat Optimally corrects chromatic aberration in the entire visible band violet red Magnification E Objective lens series list N A Numerical Aperture For brightfield observation Field Number None UIS Number None Brightfield N UIS2 Objective lens BD _ Brightfield darkfield magnification BDP Brightfield darkfield polarizing IR R LCD LCD Objective lenses series abbreviation PL Plan Series Magnification BF DF DIC POL FL F N Field Number Remarks UIS2 MPLFLN 1 25 2 5 O 1 25x 22 2 5x 26 5 Use together with polarizer and analyzer recommended 5 10 20 50 100 O OU O O 26 5 LMPLFLN 5 10 20 50 100 O OL O O 26 5 MPLN 5 10 20 50 100 O 22 LCPLFLN LCD 20 50 100 O OL 26 5 For LCD UIS MPlanApo 20 50 100 O OU O 26 5 SLMPlan 20 50 O 26 5 LMPlanIR MPlanIR 5 10 20 50 100 O 22 For near IR observation UIS2 MPLFLN BD 5 10 20 50 100 150 O O OU O O 26 5 MP
9. U MDIC3 Mirror unit for reflected DIC 80 VV NAN U MBFL3 Mirror unit for reflected brightfield 80 UN amination for high intensity light source mounting U MWUS3 Fluorescence mirror unit for 80 postion reflected U excitation U MWBS3 Fluorescence mirror unit for 80 Weight 3 8kg reflected B excitation U MWGS3 Fluorescence mirror unit for 80 reflected G excitation U RCV DF converter for BX URA2 is needed with darkfield observation Unite mi 23 ILLUMINATION UNITS Reflected light illuminators for BF BX KMA BX KMA ESD Enables brightfield Nomarski DIC and simple polarizing observations ESD model is also available Accessories Unit name Description Weight g U 25LBD LBD filter slider 20 U 251F550 IF550 filter slider 20 U 25ND6 ND filter 20 U 25ND25 ND filter 20 U 25FR Frost filter slider 20 U 25L42 UV cut filter 20 U PO3 Polarizer slider for reflected light 71 U POTP3 Polarizer slider for reflected light 71 with tint plate U AN360 3 360 rotatable analyzer slider 79 U AN Analyzer slider for reflected light 50 U DICR DIC slider for reflected light 130 U DICRH DIC slider for reflected light 130 high resolution type U DICRHC DIC slider for reflected light 130 high contrast type 84 Revolving 1 position Eri nosepiece mounting 3 5 Illuminator mounti
10. aberration Our advanced design and manufacturing techniques have realized good optical performance even with large numerical aperture 2 Coma aberration Even though spherical aberration is compensated to be very small there are cases where light rays coming out of an off axis object point are not condensed to a single point on the image plane but generate asymmetric blur just like a comet leaving traces This is called coma aberration Figure 9 4 Coma Aberration and Spot Shape on the Image Plane Specimen Aplanatic tube lens CEHE Objective lens with Image plane coma aberration 3 Astigmatism Even though a lens is compensated for spherical aberration and coma aberration there are cases where an image of an off axis object point is not focused to a single point but separated to a concentric line image and a radial line image This is called astigmatism When astigmatism is present a point image blurs vertically and horizontally before and after the focus position OPTICAL TERMINOLOGY Figure 9 5 Astigmatism and Change in Spot Shape in Different Focus Positions 4 Field curvature An image plane of an object on a plane perpendicular to an optical axis does not always become a plane perpendicular to the optical axis but it generally becomes a curved plane This symptom is called field curvature When field curvature is present the image is more displaced
11. accomplished with this component Several microscopic operations are totally controlled from an external unit by combining this component with an auto focus unit U FH Focus adjustment knob unit 341 6 Weight 7 6kg Consult your Olympus dealer about the mounting dimensions U IFFH Focus adjustment knob interface 70 Weight 760g Weight 1450g Unit mm 41 45 aa gg Sy ea DEEP ULTRAVIOLET OBSERVATION SYSTEM Deep ultraviolet observation system This module adds a deep ultraviolet 248nm optical system to a general microscope An ultra high resolution observation is executed by using an extremely short wavelength ray U UVF248IM U UVF2FB 5FB UV248 compatible intermediate tube UV quartz light guide 2000 or 5000 P 8 light guide x 108 00000Vz 30 4 9 Weight U UVF2FB 50g Cc Re U UVF5FB 80g 105 8 258 5 i 102 5 39 5 distance between mounting positions 6 Weight 1 9kg U UVF248LB U LH80HG XE UV248 compatible light source box Mercury Xenon lamp housing 45 150 30 180 170
12. as it becomes closer to the periphery of the visual field Therefore when the center of an image is brought into focus blur occurs in the peripheral areas of the image To bring the entire image including the periphery into clear focus it is necessary to adequately compensate for this type of aberration 5 Distortion When there is no similar relation between a planar shape on an object and a shape on the image plane this is called distortion When distortion is present a Square image appears in a shape of a barrel or pin cushion as shown in Figure 9 6 Figure 9 6 Distortion a Pin cushion type a Barrel shape type The microscope optical system contains some distortion When distortion is present it can bring erroneous results of shape measurements When a microscope is used for precision measurements pay close attention to this aberration for example by providing it with an aberration compensation function 6 Chromatic aberration Glasses used for optical systems have different refractive indexes depending on the wavelength This causes differences in focal length between wavelengths and generates displacement of image forming position This phenomenon is called chromatic aberration which is sometimes subdivided into axial displacement on the optical axis called axial chromatic aberration or lateral chromatic aberration and displacement on the image plane called chromatic
13. method of attaching illuminator refer to page 24 487 O 169 45 Illuminator cable length 1 800mm Weight 5 5kg exclude objective lens BX RFAA Motorized universal reflected light illuminator Reflected light fluorescence illuminator with simultaneous attachment of six mirror units Incorporates motorized mirror unit changeover and shutter Revolving nosepiece n mounting ola position s s Illuminator mounting position A Illuminator cable length 1 800mm Weight 4 3kg 135 41 261 Unit mm 371 38 MOTORIZED UNITS U D5BDREMC Motorized quintuple BD revolving nosepiece with slider slot for DIC U D6REMC Motorized sextuple revolving nosepiece with slider slot for DIC U P5REMC Motorized centerable quintuple revolving nosepiece with slider slot for DIC Motorized units including motorized il from B X UCB BX UCB Control unit uminator and auto focus unit can be totally controlled 310 332 depth OLYMPUS BX UCB N DA N N
14. of UIS2 UIS objective lenses are the values when the focal length of the tube lens is 180 mm Focal length of tube lens M ob f M op Objective lens magnification f Objective lens s focal length 43 5 Total Magnification 5 1 Observation through eyepiece binocular observation M toinoy M obxM oc M ino Total magnification for binocular observation M oby Objective lens magnification M oc Eyepiece magnification 5 2 Video monitor observation Total magnification for video monitor M wideo monitor MI opp M video camera adapterxM onitor magnification M ideo monitor Total magnification on the video monitor M oby Objective lens magnification M ideo camera adapter Projected magnification for video camera adapter including photo eyepiece refer to Figure 1 Refer to Figure 3 for Monitor magnification Practical field of view for video monitor observation Practical field of view for Image device size video monitor observation M bx M video camera adapter M oby Objective lens magnification M ideo camera adapter Projected magnification for video camera adapter including photo eyepiece refer to Figure 1 for projected magnifications Refer to Figure 2 for image device size Figure 1 Video camera adapter and projection magnifications Video camera adapter Projection lens Projection magnifications U TV1x 1 1x video camera mount adapters U TV0 63xC
15. val lt p 7 FE 8 32 lt gt 1 m L a 240 130 q OJ A The rotation angle of the horizontal arm can restrict to 90 degrees with stopper U ST Compact stand Weight 1 8kg 22 Major specifications Item Specifications 1 Diameter of focusing arm or fixing section of tube 932mm 2 Vertical pole diameter 240mm 3 Horizontal poles diameters 25mm both upper and lower poles 4 Stroke Horizontal 234mm Vertical 205mm 5 Movement range Horizontal 541 435 106 mm max Vertical pole BXFM S optical axis 6 Maximum specimen weight Forward 10kg within 90 degree area Transverse direction 6kg Backward direction 7kg at maximum stroke 7 Weight 30kg SZ STL Large stand Weight 5kg Unit mm ILLUMINATION UNITS Reflected light illuminator for BF DF BX RLA2 ND filters are linked when exchanging between brightfield and darkfield Accessories Unit name Description Weight g U 25LBD LBD filter slider 20 U 251F550 IF550 filter slider 20 U 25ND6 ND filter 20 U 25ND25 ND filter 20 U 25FR Frost filter slider 20 U 25L42 UV cut filter 20 U PO3 Polarizer slider for reflected light 71 U POTP3 Polarizer slider for reflected light 71 with tint plate U AN360 3 360 rotatable analyzer slider 79 U AN Analyzer s
16. 0 500 0 53 1 0 MPlanApo 100x 0 95 0 35 1 8 150 1000 0 22 0 67 1000 0 27 0 67 Screw W20 32x0 706 0 8 x1 36 Super Long WD M Plan Achromat SLM Plan series Plan Achromat objective lenses with high magnification and super long working distance Two magnifications 20x and 50x are available For 5x or 10x objective lenses select from the LMPLFLN series SLMPlan20x SLMPlan50x 920 32 20 32 on A vV S Y 926 fa e a z Unit mm UIS objective lenses Widefield eyepiece WHN 10x Field Number 22 Super widefield eyepiece SWH10x Field Number 26 5 Objective lens Numerical Working distance Focal distance Weight Total Practical field Depth of Total Practical field Depth of magnification Aperture mm f mm g magnifications of view mm focus um magnifications of view mm focus um SLMPlan 20x 0 35 21 0 9 73 200 1 1 7 2 200 1 3 7 2 SLMPlan 50x 0 45 15 0 3 6 91 500 0 44 2 9 500 0 53 2 9 Screw W20 32x0 706 0 8 x1 36 12 UIS OBJ ECTIVE LENSES IR Long WD M Plan SemiApochromat IR M Plan SemiApochromat LMPlan IR series MPlan IR IR objective lenses which compensate for aberrations from visible to near infrared light Ideal for the observations of semiconductor interiors and the back surface of a chip package as well as CSP bump inspection LMPlan5xIR lt 920 32 gt WD 20 LMPlan50xIR 45 39 37 5
17. 100xBD MPLFLN20xBD 4 5 MPLFLN150xBD 45 44 41 WD 1 Unit mm UIS2 objective lenses Widefield eyepiece WHN 10x Field Number 22 Super widefield eyepiece SWH10x Field Number 26 5 Objective lens Numerical Working distance Focal distance Weight Total Practical field Depth of Total Practical field Depth of magnification Aperture mm f mm g magnifications of view mm focus um magnifications of view mm focus um MPLFLN 5xBD 0 15 12 0 36 95 5 50 4 4 59 50 5 3 59 MPLFLN 10xBD 0 30 6 5 18 82 8 100 2 2 15 100 2 7 15 MPLFLN 20xBD 0 45 3 0 9 87 7 200 1 1 5 2 200 1 3 5 2 MPLFLN 50xBD 0 80 1 0 3 6 99 8 500 0 44 13 500 0 53 13 MPLFLN 100xBD 0 90 1 0 1 8 98 9 1000 0 22 0 73 1000 0 27 0 73 MPLFLN 150xBD 0 90 1 0 1 2 104 8 1500 0 15 0 6 1500 0 18 0 6 Screw W26x0 706 14 er oe eS ye Se UIS2 OBJ ECTIVE LENSES M Plan SemiApochromat BDP BDP Brightfield Darkfield P olarizing MPLFLN BDP series Plan SemiApochromat objective lenses giving high level correction for chromatic aberration The series secures a W D of 1mm or longer Since the exit pupil position of the 5x 100x objective lenses is standardized the position of the DIC prism does not have to be switched when changing the magnification The BDP series optimizing brigh
18. 2 200 1 3 5 2 LCPLFLN 50xLCD 0 70 2 5 3 6 170 500 0 44 1 6 500 0 53 1 6 LCPLFLN 100xLCD 4 0 85 0 9 1 8 185 1000 0 22 0 79 1000 0 27 0 79 Screw W20 32x0 706 0 8 x1 36 The figure shown here is the value when the correction ring indication is 0 7 11 To be available in the beginning of 2007 eee es ae ee a ES ede UIS OBJ ECTIVE LENSES M Plan Apochromat MPlanApo series Highest class Plan Apochromat objective lens that maximize performance in brightfield observation All aberrations are corrected at the highest level while providing high N A MPlanApo20x MPlanApo50x 920 32 MPlanApo100x 920 32 45 41 5 40 7 36 8 44 65 05 3 WD 0 35 T 916 5 920 2 928 Unit mm igi Widefield eyepiece WHN 10x Super widefield eyepiece SWH10x nip eblecuMe lenses Field Number 22 Field Number 26 5 Objective lens Numerical Working distance Focal distance Weight Total Practical field Depth of Total Practical field Depth of magnification Aperture mm f mm g magnifications of view mm focus um magnifications of view mm focus um MPlanApo 20x 0 60 0 9 9 150 200 11 3 7 200 13 3 7 MPlanApo 50x 0 95 0 3 3 6 150 500 0 44 1
19. 46 aberration of magnitude Many special glass materials are used e g for apochromats MPlanApo in Olympus to eliminate chromatic aberration in a wide range from violet light g rays with wavelength of 435 nm to red light c rays with wavelength of 656 nm 9 3 Wavefront Aberration Since a long time ago aberrations have been used in geometric optics which considers light as light rays Microscope optical systems are often used for observation of very small specimens at a wavelength level and sometimes adopt wave optics which regards light as waves and handles the phase information taking account of the influence of diffraction In such a case wavefront aberration is used for evaluation As shown below when requirements for ideal imaging are satisfied in a microscope optical system the spherical wavefront spherical waves coming from a single point on an object specimen is converted to plane waves through an ideal objective lens The plane waves are converted to spherical waves through an ideal tube lens and condensed to a single point The wavefront of these waves is called the ideal wavefront Figure 9 7 Ideal Microscope Optical System Ideal Specimen Ideal j tube lens objective lens i Spherical wave Image plane Plane wave Spherical wave Based on the figure indicated for 1 spherical aberration the be
20. 5 Long WD M Plan SemiApochromat BD LMPLFLN BD series 16 Plan Achromat BD PLN BD Series 17 Plan Apochromat BD PlanApo BD 18 MICROSCOPE SYSTEM BXFM 19 22 BXFM frame BXFM F 19 BXF BXFM F BXFM ILH BXFM ILHSPU 20 BXFM S BXFM F BXFM ILHS 21 Universal stand type 2 SZ2 STU2 22 Compact stand U ST 2 22 2 eee renen ence 22 Large stand SZ STL ence nee 22 ILLUMINATION UNITS 2 22 02 202222202 23 25 Reflected light illuminator for BF DF BX RLA2 eee 23 Universal reflected light illuminator BX URA2 23 Reflected light illuminators for BF BX KMA BX KMA ESD Reflected light illuminator for BF U KMAS LAMP HOUSING amp ACCESSORIES 26 28 75W xenon apo lamp housing U LH75XEAPO 26 100W mercury apo lamp housing U LH100HGAPO 26 100W mercury lamp housing U LH100HG 26 100W halogen lamp housings U LH100 3 U LH100IR U LH100L 3 26 External power supply TH4 100 200 27 Hand switch TH4 HS 27 Extensi
21. ECES Revolving nosepieces for BF objective lenses Choose from following 6 types For motorized nosepieces refer to motorized unit page U 5RE 2 U D6RE U D7RE Quintuple revolving nosepiece Sextuple revolving nosepiece with slider slot for DIC Septuple revolving nosepiece with slider slot for DIC U D6RE ESD Sextuple revolving nosepiece with slider slot for DIC with ESD treatment Weight 520g Weight 800g Weight 980g U P4RE E Centerable quadruple revolving nosepiece with Centerable sextuple revolving nosepiece with slider slot for DIC slider slot for DIC 47 2 a g TTT 1G Vi RK ANS an Weight 1kg Weight 1kg Insert the DIC dummy when not using the DIC slider Unit mm 34 ee T Sa Ed REVOLVING NOSEPIECES Revolving nosepieces for BF DF objective lenses Choose from following 3 types Use of adapter to mount BF objectives BD M AD enables attachment of brightfield objective lenses For motorized nosepieces refer to motorized unit page U 5BDRE U D5BDRE U D6BDRE Quintuple revolving nosepiece for BF DF Quintuple revolving nosepiece for BF DF Sextuple revolving nosepiece for BF DF with slider slot for DIC with slider slot for DIC U P5BDRE Centerable quintuple revolving nosepiece 114 4
22. LFLN BDP 5 10 20 50 100 O eu e og 26 5 LMPLFLN BD 5 10 20 50 100 O O OL O O 26 5 MPLN BD 5 10 20 50 100 O O 22 UIS MPlanApo BD 100 O O OU O 26 5 DIC prism U DICR UM LM position U DICRHC LM position fixed U DICRH UM position fixed 5 20x U excitation also possible 50x DIC observation not applicable MPlanIR available 100x only O Responds Optimally responds DIC Differential Interference Contrast POL Polarized light FL Fluorescence E Features of objective lens series MPLFLN series M Plan SemiApochromat P 8 Plan SemiApochromat objective lenses giving high level correction for chroma The lineup consists of 7 objective lenses ranging from 1 25x to 100x and secures a W D of 1mm or longer Since the exit pupil position of the 5x 100x objective lenses is the position of the DIC prism does not have to be switched when changing the For ultra low magnifications 1 25x 2 5x use toge reflected light illuminator LMPLFLN series Long WD M Plan SemiApochromat P 9 Long working distance Plan S for chromatic aberration Sui collision as the working dista Perfect objective glass substrate Aberrati a correction ring MPlanApo series M Highest class Pla Plan Achromat objective Two magnificatio ion the LMPLFLN Series emiApochromat objec able with samples having a height difference and nce is long Also since the exi objective lenses is standardized the position of the DIC pr
23. Numerical Working distance Focal distance Weight Total Practical field Depth of Total Practical field Depth of magnification Aperture mm f mm g magnifications of view mm focus um magnifications of view mm focus um LMPLFLN 5x 0 13 22 5 36 50 50 4 4 70 50 53 70 LMPLFLN 10x 0 25 21 0 18 54 100 2 2 18 100 2 7 18 LMPLFLN 20x 0 40 12 0 9 73 200 11 6 1 200 1 3 6 1 LMPLFLN 50x 0 50 10 6 3 6 77 500 0 44 2 5 500 0 53 2 5 LMPLFLN 100x 0 80 3 4 1 8 94 1000 0 22 0 87 1000 0 27 0 87 Screw W20 32x0 706 0 8 x1 36 ee ee R UIS2 OBJ ECTIVE LENSES M Plan Achromat MPLN series Plan Achromat objective lenses providing excellent image flatness up to F N 22 MPLN5x MPLN10x MPLN20x 920 32 920 32 9 _ n n FE SK ge Ki g 3 a e i SEN s JBE 021 i 910 5 KK a Q 3 z 16 024 MPLN50x MPLN100x 920 32 920 32 lt lt eg 3 a THERE ki F g Jes L ER 4 4 3 011 9 3 911 6 Q Q z 915 8 915 6 924 024 Unit mm geet Widefield eyepiece WHN10x UIS2 objective lenses Field Number 22 Objective lens Numerical Working distance Focal distance Weight Total Practical field of Depth of focus magnification Aperture mm f mm g magnifications view mm um M
24. OLYMPUS MICROSCOPE COMPONENTS GUIDE Your Vision Our Future Choosing The Ideal UIS2 Optics Components For Your Equipment uls2 World leading optics The wide range of Olympus components introduced here allows users in such diverse fields as research inspection and production to take advantage of the quality flexibility and outstanding optical performance of the U IS2 O ptical System That s why installing Olympus microscope components is quite simply the right choice for your equipment EA CONTENTS WELCOME TO UIS2 UIS OPTICS 3 4 SYSTEM DIAGRAM 200 220 2220 2 eee eee 5 6 UIS2 UIS OBJ ECTIVE LENSES 7 18 Plan SemiApochromat PLFLN Series 8 Long WD M Plan SemiApochromat LMPLFLN series 9 Plan Achromat PLN Series 10 LCD Long WD M Plan SemiApochroma LCPLFLN LCD series 11 Plan Apochromat PlanApo series 12 Super Long WD M Plan Achromat SLMPlan series 12 R Long WD M Plan SemiApochromat LMPlan IR series 13 R M Plan SemiApochromat Plan IR 13 Plan SemiApochromat BD PLFLN BD series 14 Plan SemiApochromat BDP PLFLN BDP series 1
25. OMETER EYEPIECE 33 Widefield eyepieces WHN10x WHN10x H CROSSWHN10x WH15x 33 Super widefield eyepieces SWH10x H MICROSWH10x CROSSSWH1Ox 33 Filar micrometer eyepiece U OSM 33 REVOLVING NOSEPIECES wo teen tees ee cence ee ccs nce ee eeceeees 34 35 Quintuple revolving nosepiece U 5RE 2 34 Sextuple revolving nosepiece with slider slot for DIC U D6RE 34 Sextuple revolving nosepiece with slider slot for DIC with ESD treatment U D6RE ESD 34 Septuple revolving nosepiece with slider slot for DIC U D7RE 34 Centerable quadruple revolving nosepiece with slider slot for DIC U P4RE 34 Centerable sextuple revolving nosepiece with slider slot for DIC U P6RE 34 Quintuple revolving nosepiece for BF DF U 5BDRE 35 Quintuple revolving nosepiece for BF DF with slider slot for DIC U D5BDRE 35 Sextuple revolving nosepiece for BF DF with slider slot for DIC U D6BDRE 35 Centerable quintuple revolving nosepiece U P5BDRE 35 Adapter to mount BF objective
26. P1xC Dual port 1x Combine with U DP to obtain a 1x image 17 53 lt gt 244 025 L X 230 244 1 32UN 170 5 mount face 182 4 5 Weight 500g U APT Arrow pointer Projects an arrow into the field of view 45 0 92 tolerence from light axis 120 Weight 1 2kg Unit mm 32 ee en ea ee as ee EYEPIECES FILAR MICROMETER EYEPIECE Eyepieces Eyepieces for UIS2 optical system WHN10x WHN10x H WH15x SWH10x H Widefield eyepiece CROSSWHN10x Widefield eyepiece MICROSWH10x Widefield eyepieces CROSSSWH10x Super widefield eyepieces 046 2 941 lt a 941 943 2 038 5 038 5 936 5 033 rc EP BE EESE o i o g g lt g a A g a WEN10 H 2267 o a 8 g pa ii o Ne j x en j I o iw ar 030 g Diopter i Hk Field Micrometer Weight Name Number adjustment range giameter mm 9 Remarks WHN10x 22 24 90 WHN10x H 22 8 45 24 170 With adjustable diopter CROSSWHN10x 22 8 5 170 With cross lines and adjustable diopter WH15x 14 24 90 SWH10x H 26 5 8 2 210 With adjustable diopter MICROSWH10x 26 5 8 2 210 With micrometer and adjustable diopter CROSSSWH10x 26 5 8 2 210 With cross lines and
27. PLN 5x 0 10 20 0 36 64 50 4 4 98 MPLN 10x 0 25 10 6 18 80 100 2 2 18 MPLN 20x 0 40 1 3 9 111 200 11 6 1 MPLN 50x 0 75 0 38 3 6 113 500 0 44 1 4 MPLN 100x 0 90 0 21 1 8 116 1000 0 22 0 73 Screw W20 32x0 706 0 8 x1 36 10 B oe eS ye Se UIS2 OBJ ECTIVE LENSES LCD Long WD M Plan SemiApochromat LCPLFLN LCD series Perfect objective lens series for observation of LCD panels and other samples through a glass substrate Aberration correction matched to the glass thickness is accomplished using a correction ring LCPLFLN20xLC D LCPLFLN50xLC D 45 45 238 LCPLFLN100xLC D Unit mm Value at glass thickness 0 7mm observation Objective lens LCPLFLN20xLCD LCPLFLN50xLCD LCPLFLN100xLCD Corresponding glass thickness mm 0 1 2 0 1 2 0 0 7 Correction ring indication 0 0 7 1 2 0 0 7 1 2 0 0 5 0 7 Working distance mm 8 3 7 8 7 4 3 0 2 5 2 2 1 2 0 98 0 9 Correction system Correction ring Correction ring Correction ring eae Widefield eyepiece WHN10x Super widefield eyepiece SWH10x IS objectivelenses Field Number 22 Field Number 26 5 Objective lens Numerical Working distance Focal distance Weight Total Practical field Depth of Total Practical field Depth of magnification Aperture mm f mm g magnifications of view mm focus um magnifications of view mm focus um LCPLFLN 20xLCD 0 45 7 8 9 146 200 11 5
28. STEM DIAGRAM Observation tubes Refer to pages 29 30 0 Ic U AN U AN360 3 Intermediate tubes Refer to pages 31 32 GC A U PO3 OMG U POTP3 Refer to pages 38 40 U AFAIM U FWO FLE OO IC U AN360RAF E Os U 25ND6 U 25ND25 U 25LBD Mirror units Yq U 251F550 U 25L42 U 25FR U DICR U DICRH 3 U DICRHC BX REMCB is also available for BX RLAA mo orized revolving nosepiece control refer to page 39 Refer to page 26 U LH75XEAPO U LH100HG ia U LH100 3 U LH100L 3 U LH100HGAPO U ZPCB Z board BX2BSW Control software BXFM A SYSTEM DIAGRAM Video system Refer to pages 37 38 Observation tubes Refer to pages 29 30 BXFMA F TAS U D6REMC U P5REMC U D5BDREMC Objective lenses U FWR 32filter O provided with the BXFMA F E Auxiliary lens U LH100HG U LH75XEAP 0 Refer to page 41 U LH100 3 U LH100L 3 BX UCB U ZPCB Z board PC B U HSTR2 Power source UIS2 UIS OBJ ECTIVE LENSES E Meaning of abbreviations shown on objective lens M P L Plan FLN 1 00 Me B D pa E Objective lens notation
29. Weight 1 0kg Extension cord U RMT 1700mm should be used to connect the lamp housing U LH100 3 to the BX UCB BX REMCB Control box for motorized nosepiece and BF DF illuminator BX RLAA and U D5BDREMC U D6REMC U P5REMC can be controlled from U HSTR2 or direct from the computer keyboard via an RS232C connector BX RFAA and U D5BDREM U D6REM combination not applicable 190 4 39 8 144 39 Weight 1 1kg U HSTR2 Hand switch 105 Cable length 2000mm Weight 370g U ACAD4515 AC adapter for BX REMCB 2000 109 am o y 129 5 1 Unit mm MOTORIZED UNITS U AFA1M Active auto focus unit 312 5 147 5 135 Weight 1 0kg U FWR o ON HION 1 Z000 WIVIV N 148 170 5 Cable length 2000mm Weight 3 3kg Motorized reflected filter wheel Accomplish maximum 6 filter position exchange 180 5 40 Consul your Olympus dealer about the motorized focus Unit mm MOTORIZED UNITS BXFMA F Motorized illumination with power focus A motorized microscope unit for integration with your equipment Motorized operations such as revolving nosepiece up down objective lens switching aperture diaphragm open close and brightfield darkfield switching are
30. adjustable diopter Unit mm EP eyepoint Filar micrometer eyepiece U OSM Used for precise measurement in the field of view 137 129 2 23 5 117 8 Eyepiece Magnification 10X erect image inverted when used with erect image observation tube F N 14 Diopter adjustment range 5 1 m Provided with rubber eye shade 30 108 614 2 0 Mounting position inside e 8 or 29 5 75 5 Weight 580g 33 Measuring scale Scale lines graduated in increments of 1mm in the entire 10mm length Shift of scale lines 1mm per rotation of the shift ring the circumference of which is divided into 100 graduations Measuring range 10mm objective lens magnification Compensation limit for objective lens magnification tolerance 5 by combined use of the zoom compensation ring and the provided stage micrometer Compensation ring clamping screw Magnification compensation scale Actual size Measured value mm Objective lens magnification Actual size mm Repeatability Repeatability error 7 mm A Objective lens magnification Accuracy Measuring error A Objective lens magnification L Measured length in mm 0 0002XA 0 002 L 0007 m Unit mm ee eT Se ee ees REVOLVING NOSEPI
31. allation Weight 315g U LGAD Fiber adapter for reflected light observation 51 dimension lt os I for installation Light guide mount hole 12 Weight 390g 27 Weight 200 g SZX TLGAD Transmitted light guide adapter Mountable with BX KMA BX KMA ESD only Light guide mount hole 12 Weight 135g Unit mm ee ee ee See eee LAMP HOUSING amp ACCESSORIES LG PS2 Light source 915 Light guide mounting position 8 251 The types of model varies by country in use Weight 1 6kg LG SF Light guide 10 Groove Width3 Depth1 15 Weight 210g U DULHA Double lamp house adapter 88 171 28 Weight 1 2kg Unit mm pT a ae OBSERVATION TUBES Widefield trinocular observation tubes Trinocular observation tubes with widefield of view Compatible with F N 22 92 5 IR 93 9 U TR30 2 Widefield binocular tube U TR30IR Widefield binocular tube for IR 175 59 65 18 U ETR 4 Widefield erect image trinocular tube 92 120 33 5 16 Unit mm Field Number Inclination angle Interpupillary distance Light path selector An Weight Name F N degree mm eyepiece vide
32. by changing the Objective lens and telan distance This makes it possible to use a distance of 50mm to 170mm from objective lens mounting position to the single port tube with lens Coma aberration refer to the optical terminology at the end of this document Figure 3 Basic dimensions of UIS2 UIS2 optical system U TLU Single port tube with lens Objective lens Image eee LP so ian Flea 40mm 84mm 45mm Recommended distance 57 6mm 102mm a 50 170mm a ca Basic dimensions when our revolving nosepiece and illuminator are combined When the position of the illuminator above is changed illumination performance cannot be maintained O a EEG eT Say WELCOME TO UIS2 UIS OPTICS Features of UIS2 objective lenses UIS2 objective lenses ensure compatibility screw diameter optical performance with the UIS optical system and have the following features compared to conventional objective lenses 1 Wavefront aberration control The Olympus UIS2 objective lenses set a new standard with wavefront aberration control in addition to common performance standards of N A and W D Olympus challenges farther highest order optics which has not been fulfilled by the conventional standards We offer excellent performance objective lenses by minimizing the aberrations that lower resolution Wave front aberration refer to the optical terminology at the end of this document 2 Objectiv
33. corrected optics This system known as infinity corrected optics offers a number of advantages e There is no change in magnification even when the distance between the objective lens and tube lens is altered e With the total magnification remaining constant there is no image aberration even when prisms or Sliders are interposed between the Objective lens and the tube lens As thousands of users have found by experience these advantages are crucial to composing the ideal microscope optical system What s more it is even possible to freely insert or remove intermediate attachments in the parallel rays of light between the objective lens and tube lens allowing the creation of user specific or task specific optical systems To establish real flexibility with such a system it is necessary to eliminate the occurrence of coma aberration n UIS2 UIS objective lenses the parfocal distance is designed at 45mm and the focal length of the tube lens is 180mm Figure 2 Advantages of Infinity corrected optical system Infinity corrected optical system Objective Tube lens Objective lens lens Finite corrected optical system Basic dimensions of UIS2 UIS optical system The UIS2 UIS optical system optimally corrects aberration with a dedicated telan lens and an eyepiece so that the coma aberration and flatness are not degraded even when the telan lens exit pupil position is changed
34. d light 130 high contrast type 64 21 21 155 198 25 Weight 1 2kg Unit mm E Eee LAMP HOUSING amp ACCESSORIES Lamp housings Various different lamp housings are available for use with different light sources choose to suit the intended purpose U LH75XEAPO U LH1OOHGAPO 75W xenon apo lamp housing 100W mercury apo lamp housing U LH100HG 100W mercury lamp housing 148 5 30 2 83 5 148 5 30 2 93 i 115 75 169 depth dimension for installation 180 5 169 depth dimension for installation Cable length 2 000mm Accepted lamp UXL 75XB Weight 3 1kg P ower supply unit AH2 RX T or U RX T200 and power cable UY CP are necessary for 180 5 75W xenon lamp housing These items are sold separately Cable length 2 000mm Accepted lamp USH 1030 L Weight 2 7kg Power supply unit BH2 RFL T3 or U RFL T200 and power cable UYCP are necessary for 100W mercury lamp housings These items are sold separately AH2 RX T dimensions 120 W x290 D x186 H weight approx 4kg BH2 RFL T3 dimensions 120 W x290 D x225 H weight approx 5kg U RX T200 for EU countries dimensions 115 W x195 D x260 H weigh
35. e lenses with excellent image parcentricity High power SemiApochromatic UIS2 objective lenses make the centration tolerance between objective lenses on the microscope nosepiece keep the image within the enter of the field of view even with digital cameras 50x or higher power in both MPLFLN and LMPLFLN series 3 Improvement of color reproducibility UIS2 objective lenses realize natural color reproduction without any chromatic shifts using stringently selected high transmittance glass and advanced coating technology that provides high transmittance which is flat over an ultra wide band wavelength In addition since the total optical system including the tube lens is designed to reproduce a natural color clear images faithful to the specimen are obtained even with digital imaging 4 Lightening Weight has been reduced to approximately 2 3 that of conventional products by using an aluminum objective lens barrel cover This has the effect of lightening the load on the devices at Objective lens up down suppressing vibrations by lowering the inertial force at objective lens Switching etc MPLFLN series LMPLFLN series Adoption of eco lens The glass materials of UIS2 objective lenses are all lead and cadmium free eco glass Based on our conviction that the UIS2 UIS system is the best way to maximize the advantages of infinity corrected optical systems we confidently recommend the UIS2 UIS featured Olympus microscope unit
36. f the specimen layer which is in sharp focus at the same time even if the distance between the objective lens and the specimen plane is changed when observing and shooting the specimen plane by microscope As human eyes are individually different in the ability of their focus adjustment each person s perception of the focal depth varies At present the Berek formula is generally used because it gives a focal depth value that often coincides with that obtained through experiments Focal depth formula Visual observation Berek formula x 250 000 N A xM D 0 F D O F Depth Of Focus Resolving power of eyes 0 0014 when optical angle is 0 5 degrees M Total magnification objective lens magnification x eyepiece magnification 0 275 N A A 0 55um This indicates that the focal depth becomes smaller as the numerical aperture becomes larger Example With MPLFLN100x N A 0 90 WHN10x 350 0 275 0 90x 1 000 0 81 D 0 F 0 39 0 34 0 73 um Video camera In the case of a video camera the focal depth will vary according to number of pixels of CCD optical magnification and numerical aperture The above mentioned formula is used as a rough guide only Ss ee eT ee a ee OPTICAL TERMINOLOGY 9 Aberrations A difference between an ideal image and an actual image that passes through an optical system is called an aberration 9 1 Requirements for Ideal Image Formation The followin
37. g the m MPLN BD series Plan Achromat objec MPlanApo BD Plan Apochromat BD P 18 ive le rdized ification objective lenses giving high jon Suitable with sa agnification M Plan Achromat BD P BF Brightfield DF Darkfield of 1mm or longer Since M Plan SemiApochrom of 1mm or longer Since ics is perfect for Nomarsk n SemiApochromat objec stance is long Also sinc he position of the DIC prism do he position of the DIC prism do agnification The BDP series optimizing bri and the bac tBD P1 level correcti the exit pupi level correcti the exit pupi LMPlan IR series IR Long WD M Plan SemiApochromat P 13 MPlan IR IR M Plan SemiApochromat P 13 IR objective lenses which compensate for aberra Ideal for the observations of semiconductor interiors as well as CSP bump inspection MPLFLN BD series M Plan SemiApochroma i jective lenses giving high ions from visible to near infrared light surface of a chip package 4 jon for chromatic aberration il position of the 5x 150x es not have to be switched at BDP P15 jon for chromatic aberration il position of the 5x 100x es not have to be switched ghtfield darkfield and DIC and pol an SemiApochromat BD ive lenses g mples having a height e the exit pu 17 nses providing excellent i mage flatnes arized light observations P16 iving high level co
38. g three requirements must be satisfied to form an image with no aberration or an ideal image i All the light rays coming from a single point and passing through an image formation optical system converge on a single point i Image points which correspond to object points on the same plane perpendicular to the optical axis are present on the same plane ii The planar shape of an object and the planar shape of an image that are on the same plane perpendicular to the optical axis have a similarity relation Figure 9 1 Requirements for Ideal Image Formation oi Object Image plane D ig O iig In an actual optical system however it is very difficult to strictly meet the requirements for ideal image formation and this causes aberrations that interfere with image forming performance 9 2 Classification of Aberrations Aberrations that interfere with image forming performance are Classified as shown below in Figure 9 2 Seidel s aberration Expansion of a point image Curvature of image plane Deformation Figure 9 2 Classification of Aberrations 1 Spherical aberration 2 Coma aberration Seidel s aberration 3 Astigmatism 4 Field curvature Aberration __ 5 Distortion 6 Longitudinal axial chromatic aberration Chromatic aberration 7 Chromatic aberrati
39. havior of the wavefront in an optical system that has an aberration is described below Figure 9 8 Illustration of Wavefront Aberration Actual wavefront Specimen SO Ideal Pe wavefront Objective lens with spherical aberration A difference a degree of disagreement between the ideal wavefront and the actual wavefront shown above is called wavefront aberration OLYMPUS CORPORATION has obtained IS09001 14001 Specifications are subject to change without any obligation on the part of the manufacturer Ol YM PU Ss OLYMPUS CORPORATION Shinjuku Monolith 3 1 Nishi Shinjuku 2 chome Shinjuku ku Tokyo Japan OLYMPUS LIFE AND MATERIAL SCIENCE EUROPA GMBH Postfach 10 49 08 20034 Hamburg Germany OLYMPUS SURGICAL amp INDUSTRIAL AMERICA INC One Corporate Drive Orangeburg NY 10962 U OLYMPUS UK LTD www olym pus com 2 8 Honduras Street London EC1Y OTX United Kingdom OLYMPUS AUSTRALIA PTY LTD 31 Gilby Road Mt Waverley VIC 3149 ANG Australia OLYMPUS LATIN AMERICA 5301 Blue Lagoon Drive Suite 290 Miami FL ING U S A Printed in Japan M1606E 1106B
40. image ae U SWTR 3 26 5 24 50 76 100 0 20 80 0 100 Inverted 2300 U SWETR 26 5 24 50 76 100 0 0 100 Erect 4200 MX SWETTR 26 5 0 42 50 76 100 0 0 100 Erect 4200 Length marked with an asterisk may vary according to interpupillary distance The distance for figure shown is 62mm 30 a aS eS ee ee ee INTERMEDIATE TUBES amp ACCESSORIES Intermediate tubes Various accessories for various observation need U CA Magnification changer Provides 1x 1 2x 1 6x and 2x intermediate magnifications ict 2 Weight 1 3kg U TRU Trinocular intermediate attachment Intermediate attachment which divides the light path allowing attachment of both digital and video cameras 183 9 BI PT 100 0 20 80 Weight 1 3kg U ECA Magnification changer 2x Provides 1x and 2x intermediate magnifications 88 Weight 1 3kg Unit mm a FE ENE EO SEE INTERMEDIATE TUBES amp ACCESSORIES U DP Dual port Use this intermediate tube to divide the light path 88 151 51 mount face 38 Weight 1kg Light path selector by mirror unit Transmitted side port side port 100 0 07 Transmitted side port side port 70 30 with use of U MBF3 U EPA2 Eyepoint adjuster Raises eyepoint by 30mm Weight approximately 500g U D
41. ion with power focus BXFMA F 2 22 222 2222 2222 41 DEEP ULTRAVIOLET OBSERVATION SYSTEM 42 UV248 compatible intermediate tube U UVF248IM 42 UV quartz light guide U UVF2FV 5FB 42 UV248 compatible light source box Mercury Xenon lamp housing U UVF248LB U LH80HBXE 42 OPTICAL TERMINOLOGY 43 46 i ee ae ee ee a WELCOME TO UIS2 UIS OPTICS UIS2 UIS The System That Maximizes The Advantage Of Infinity C orrected Optics What s infinity corrected optics UIS2 UIS optics is an infinity corrected optical system in other words a system in which light passes from the specimen through the objective lens without forming an image along the way Instead it travels in the form of infinity parallel rays to the tube lens The tube lens is where the intermediate image is formed whereas in finite corrected optics this is done by the objective lens Figure 1 Infinity corrected and finite corrected optical system principles Ss finity corrected optical system Parallel light beam Eyepiece CO i UIS UIS2 Tube lens Intermediate objective lens image Finite corrected optical system Eyepiece STI ii Objective Intermediate lens image Advantages of infinity
42. ism does not have to be switched P when changing the magnifica MPLN series M Plan Achromat P 10 Plan Achromat objective lenses providing excellent image fla LCPLFLN LCD series LCD Long WD M Plan SemiApochromat P 11 ens series for observation of LCD panels and other samples through a on correction matched to the glass Plan Apochromat P n Apochromat objective lenses thai observation All aberrations are corrected at the highest level while providing high N A SLMPlan series Super Long WD M Plan Achromat P 12 enses with high magnifica ns 20x and 50x are available For 5x or 10x objective lenses selec ic aberration standardized magnification her with analyzer and polarizer of the ive lenses giving high level correction objective pupil position of the 5x 100x objective polarized hickness is accomplished using 12 maximize performance in brightfield ion and super long working distance from Plan SemiApochromat ob The series secures a W D lenses is standa in preventing when changing the magn MPLFLN BDP series an SemiApochroma The series secures a W D lenses is standardized ness up to F N 22 when changing the m ight characteris LMPLFLN BD series Long WD M PI Long working distance Pla for chromatic aberrati collision as the working di objective lenses is standardized the position of the DIC prism does not have to be switched when changin
43. itable with samples having a height difference and in preventing collision as the working distance is long Also since the exit pupil position of the 5x 100x objective lenses is standardized the position of the DIC prism does not have to be switched when changing the magnification LMPLFLNSxBD 926 30 26 6 45 26 2 WD 15 LMPLFLN10xBD LMPLFLN100xBD LMPLFLN20xBD Unit mm UIS2 objective lenses Widefield eyepiece WHN 10x Field Number 22 Super widefield eyepiece SWH10x Field Number 26 5 Objective lens Numerical Working distance Focal distance Weight Total Practical field Depth of Total Practical field Depth of magnification Aperture mm f mm g magnifications of view mm focus um magnifications of view mm focus um LMPLFLN 5xBD 0 13 15 0 36 81 50 4 4 70 50 5 3 70 LMPLFLN 10xBD 0 25 10 0 18 84 100 2 2 18 100 2 7 18 LMPLFLN 20xBD 0 40 12 0 9 86 200 1 1 6 1 200 1 3 6 1 LMPLFLN 50xBD 0 50 10 6 3 6 85 500 0 44 2 5 500 0 53 2 5 LMPLFLN 100xBD 0 80 3 3 1 8 102 1000 0 22 0 87 1000 0 27 0 87 Screw W26x0 706 16 UIS2 OBJ ECTIVE LENSES M Plan Achromat BD MPLN BD series Plan Achromat objective lenses providing excellent image flatness up to F N 22
44. l field Depth of Total Practical field Depth of magnification Aperture mm f mm g magnifications of view mm focus um magnifications of view mm focus um MPLFLN 1 25x 0 04 3 5 145 122 12 5 17 6 870 MPLFLN 2 5x 0 08 10 7 72 106 25 8 8 220 25 10 6 220 MPLFLN 5x 0 15 20 0 36 51 5 50 4 4 59 50 5 3 59 MPLFLN 10x 0 30 11 0 18 68 1 100 2 2 15 100 2 7 15 MPLFLN 20x 0 45 3 1 9 70 4 200 11 5 2 200 1 3 5 1 MPLFLN 50x 0 80 1 0 3 6 89 9 500 0 44 1 3 500 0 53 1 3 MPLFLN 100x 0 90 1 0 1 8 90 9 1000 0 22 0 73 1000 0 27 0 73 Screw W20 32x0 706 0 8 x1 36 To be available in the beginning of 2007 er oe eS ye Se UIS2 OBJ ECTIVE LENSES Long WD M Plan SemiApochromat WD Working Distance LMPLFLN series Long working distance Plan SemiApochromat objective lenses giving high level correction for chromatic aberration Suitable with samples having a height difference and in preventing collision as the working distance is long Also since the exit pupil position of the 5x 100x objective lenses is standardized the position of the DIC prism does not have to be switched when changing the magnification LMPLFLN5x LMPLFLN50x 45 LMPLFLN10x 24 45 WD 21 LMPLFLN100x LMPLFLN20x Unit mm UIS2 objective lenses Widefield eyepiece WHN10x Field Number 22 Super widefield eyepiece SWH10x Field Number 26 5 Objective lens
45. lider for reflected light 50 U DICR DIC slider for reflected light 130 U DICRH DIC slider for reflected light 130 high resolution type U DICRHC DIC slider for reflected light 130 Revolving high contrast type mounting ms position KA oOo Re ri ew a P haa va 45 position m 265 335 Weight 3 4kg Universal reflected light illuminator BX URA2 Suitable for observations ranging from brightfield to fluorescence Six mirror units can be attached to this reflected light illuminator simultaneously Accessories Unit name Description Weight g U 25LBD LBD filter slider 20 U 251F550 IF550 filter slider 20 U 25ND6 ND filter 20 U 25ND25 ND filter 20 a U 25FR Frost filter slider 20 a U 25L42 UV cut filter 20 U PO3 Polarizer slider for reflected light 71 U POTP3 Polarizer slider for reflected light 71 with tint plate U AN360 3 360 rotatable analyzer slider 79 U AN Analyzer slider for reflected light 50 U DICR DIC slider for reflected light 130 U DICRH DIC slider for reflected light 130 high resolution type ilies U DICRHC _ DIC slider for reflected light 130 a FT TT high contrast type i U MBF3 Mirror unit for reflected brightfield 80 hosepiece U MDF3 Mirror unit for reflected darkfield 80 Meden g
46. ng position Cable length 260 5mm 312 5 Combine SZX TLGAD when using fiber illumination Weight 3 1kg Unit mm Convex section 2 4 for positioning MOUNTING DIMENSIONS OF ILLUMINATORS BX RLA2 BX URA2 and BX KMA BX KMA ESD 21 100 40 1 170 Distance to the light axis 36 0 1 2 5 5 4 M5 depth12 or more f N nas a 5 S t n g H a f j i i g g j 3 8 i N TE o i 5 g 2 aa BENE on a ae a fo 2 O Location face 82 Revolving nosepiece relief dimension Fix illuminator using four M5 screws and projection for fastening Unit mm 24 Reflected light illuminator for BF ee ee ee eee LAMP HOUSING amp ACCESSORIES U KMAS Very compact reflected light illuminator with reduced depth Accessories Unit name Description Weight g U 25LBD LBD filter slider 20 U 251F550 IF550 filter slider 20 U 25ND6 ND filter 20 U 25ND25 ND filter 20 U 25FR Frost filter slider 20 U 25L42 UV cut filter 20 U PO3 Polarizer slider for reflected light 71 U POTP3 Polarizer slider for reflected light 71 with tint plate U AN360 3 360 rotatable analyzer slider 79 U AN Analyzer slider for reflected light 50 U DICR DIC slider for reflected light 130 U DICRH DIC slider for reflected light 130 high resolution type U DICRHC DIC slider for reflecte
47. o port Observation image g U TR30 2 22 30 50 76 100 0 20 80 0 100 Inverted 1600 U TR30IR 22 30 50 76 100 0 0 100 Inverted 1600 U ETR 4 22 30 50 76 100 0 0 100 Erect 1900 Length marked with an asterisk may vary according to interpupillary distance The distance for figure shown is 62mm Single port tube with lens When the visual observation is not needed and only video observation is required a single port tube with a built in telan lens can be attached directly to the video port U TLU U TLUIR Single port tube with lens Single port tube with lens for IR Weight 350g e For attachable video camera adapters refer to video camera adapters system diagram page pages 5 6 29 Unit mm Super widefield trinocular observation tubes OBSERVATION TUBES Trinocular observation tubes with super widefield of view Compatible with F N 26 5 U SWTR 3 Super widefield trinocular tube MX SWETTR U SWETR Super widefield erect image trinocular tube 76 4 224 Super widefield erect image tilting trinocular tube 117 223 318 527 149 081 2 837 328 338 337 718 Unit mm Name faye sad ar ee salary aaa ale a Observation
48. o the performance of objective lens resolving power focal depth and brightness The N A is determined by the following formula N A n x sine n Refraction rate of the medium between specimen and objective lenses Air n 1 oil n 1 515 6 Angle which is made by the optical axis and refraction of the light farthest from the center of the lens The visual field brightness B of the microscope is determined by the following formula in relation to the objective lens magnification M The larger the N A and the lower the objective magnification brightness will increase in the factor of the second power N A M B Numerical aperture Objective i l S 0 D Sample surface 7 Resolving Power The resolving power of an objective lens is measured by its ability to differentiate two lines or points in an object The greater the resolving power the smaller the minimum distance between two lines or points that can still be distinguished The larger the N A the higher the resolving power 44 Resolving power formula The following formula is generally used for determing resolution e 0 61 x Reyleigh formula Wavelength or radiation in use A 0 55um is used for visible light N A Objective lens N A Example MPLFLN100x N A 0 90 A 0 55um 0 3355 e 0 61 x NA NA 0 3355 0 90 0 37um 8 Focal depth of Microscope The focal depth refers to the depth o
49. on of magnification Types 1 to 3 correspond to expansion of a point image that goes against requirement i for ideal image formation in Figure 9 1 Type 4 corresponds to curvature of image plane that goes against requirement ii in Figure 9 1 Type 5 corresponds to deformation that goes against requirement iii in Figure 9 1 Types 6 and 7 correspond to color blur of images caused by 45 characteristics of glass materials used for the optical system Expansion of a point image can also be expressed by wavefront aberration that regards the light as waves and takes account of the phase to include the influence of diffraction 1 Spherical aberration When light rays coming out of an axial object point enter a lens the light rays with a larger numerical aperture N A are subjected to stronger refraction power and cross the optical axis in positions with larger differences from the ideal image formation position The aberration caused this way by different image forming positions due to differences in N A of axial light rays is called spherical aberration Spherical aberration is proportional to the cube of N A Figure 9 3 Spherical Aberration Specimen Aplanatic tube lens Objective lens with spherical aberration Image plane It is said that objective lenses with larger N A have better resolution but worsen spherical
50. on cord U MT 27 DF converter for BX URA2 U RCV eee 27 Fiber adapter for reflected light observation U LGAD 27 Transmitted light guide adapter SZX TLGAD 27 Light source LG PS2 28 Light guide LG SF 28 Double lamp house adapter U DULHA 28 OBSERVATION TUBES we teen seen cee 1515 ences nce eee ee eees 29 30 Widefield binocular tube U TR30 2 2 2 22 2222 renen eee ences 29 Widefield binocular tube for IR U TR30IR ence eee 29 Widefield erect image trinocular tube U ETR 4 29 Single port tube with lens U TLU Single port tube with lens for IR U TLUIR Super widefield trinocular tube U SWTR 3 Super widefield erect image trinocular tube U SWETR 30 Super widefield erect image tilting trinocular tube X SWETTR 30 INTERMEDIATE TUBES amp ACCESSORIES 31 32 Magnification changer U CA 31 Magnification changer 2x U ECA 31 Trinocular intermediate attachment Dual port Dual port 1x Eyepoint adjuster Arrow pointer EYEPIECES FILAR MICR
51. rally the video camera adapter is focused by switching to a low magnification after focusing at a high magnification objective lens 36 ee ee a Er VIDEO CAMERA ADAPTERS Video camera mount adapters Allows attachment to video cameras with C Bayonet Sony and F mounts Use with the U TV1x 2 Focus by amount of screwing into U TV1x 2 U BMAD U SMAD U CMAD3 Bayonet mount adapter Sony mount adapter C mount adapter 44 5 Image plane Image plane Weight 80g Weight 90g U TMAD U FMT T mount adapter F T mount adapter Image plane Image plane 46 5 S5 M42X0 75 I 954 7 Weight 70g Weight 30g It must be combined with U TMAD Unit mm Video camera port This port can be attached directly to the trinocular observation tube as well as to the single port tube with lens U TV1x 2 Video port 1x Weight 150g Unit mm 37 ee ee ee er MOTORIZED UNITS Motorized units Various motorized units perfect for automation of equipment are available BX RLAA U D6REMC U LH100 3 Motorized BF DF reflected light illuminator motorized Nomarski DIC sextuple revolving nosepiece 100W halogen lamp housing Enables motorized exchange of objective lenses selection between brightfield and darkfield observations as well as aperture diaphragm closing opening The BX UCB control unit has an RS232C connector allowing control via a PC For
52. rrection difference and in preventing pil position of the 5x 100x s up to F N 22 Highest class Plan Apochromat objective lens that maximize performance in brightfield and dar high N A field observations All aberrations are corrected at the highest level while providing ee r es R UIS2 OBJ ECTIVE LENSES M Plan SemiApochromat MPLFLN series Plan SemiApochromat objective lenses giving high level correction for chromatic aberration The lineup consists of 7 objective lenses ranging from 1 25x to 100x and secures a W D of 1mm or longer Since the exit pupil position of the 5x 100x objective lenses is standardized the position of the DIC prism does not have to be switched when changing the magnification For ultra low magnifications 1 25x 2 5x use together with analyzer and polarizer of the reflected light illuminator MPLFLN1 25x MPLFLN2 5x MPLFLN5x MPLFLN10x 028 49 49 45 41 5 45 34 3 31 6 WD 10 7 WD 3 5 930 MPLFLN20x MPLFLN50x MPLFLN100x 920 32 4 5 48 48 41 78 45 45 44 45 44 42 61 i J z z Unit mm VISZ objective lenses an ee u a Objective lens Numerical Working distance Focal distance Weight Total Practica
53. s BD M AD 35 VIDEO CAMERA ADAPTERS 36 37 C mount video camera ports U TV0 25xC U TV0 35xC 2 U TV0 5xC 3 U TV0 63XC 36 Video camera mount adapters U CMAD3 U BMAD U SMAD U TMAD U FMT 37 Video camera port VANYA D EE E dias 37 MOTORIZED UNITS 2 nenon uninin ninen nenn nee eee 38 41 otorized centerable quintuple revol otorized BF DF reflected light illuminator motorized Nomarski DIC sextuple revolving nosepiece 100W halogen lamp housing BX RLAA U D6REMC U LH100 3 38 otorized universal reflected light illuminator BX RFAA eee 38 otorized quintuple BD revolving nosepiece with slider slot for DIC D5SBDREMC 2 2 202erereeeoees 39 otorized sextuple revolving nosepiece with slider slot for DIC D6REMC 39 ving nosepiece with slider slot for DIC P5REMC Control unit BX UCB Hand switch U HSTR2 eee Control box for motorized nosepiece and BF DF illuminator BX REMCB 39 AC adapter for BX REMCB U ACAD4515 39 Active auto focus unit U AFAIM noes 40 Motorized reflected filter wheel U FWR 40 Motorized illuminat
54. s for all your high precision needs in research inspection and production equipment Refer to the Olympus home page for detailed objective lenses specifications SYSTEM DIAGRAM BXFM SYSTEM DIAGRAM Video system Refer to pages 36 37 Video camera Video camera Video camera Video camera C mount B mount 2 3 S mount 2 3 F mount U FMT U CMAD3 U BMAD U SMAD U TV0 25xC CS U TV0 35xC 2 E U TV0 5xC 3 U TV0 63xC FE U TV1x2 Observation single tubes Refer to pages 29 30 1 and eyepieces i i i i WHN i i Eyepieces Eyepieces Super widefield trinocular i q observation tubes i i Illumination systems and power supply om Co ER UDE DES MANES TA U P03 U POTP3 i i Revolving nosepiece U AN360IR T U POIR I l U BP1100IR U BP1200IR Refer to pages 34 35 i i F U 25L42 U 25FR i i F i SZX TLGAD BX KMA BX KMA ESD U LGAD BXFM ILHSPU s U LH75XEAPO RESENS STRESS ES S Pees ec none aCe SEER ea a Focusing units Refer to pages 19 21 d U LH100 3 TH4 100 BXFM ILHS U LH100L 3 BXFM ILH U LH100IR 1 d 4 U LH100HGAPO d U LH100HG d 1 i Refer to page 22 SZ STL U ST SZ2 STU2 Different types may be offered in each area ee ee ee ee ees SYSTEM DIAGRAM MOTORIZED UNIT SY
55. t approx 3kg Note Supplied by Olympus Life and Material Science Europa GmbH and its business partners U RFL T200 for EU countries dimensions 150 W x295 D x200 H weight approx 4 8kg U LH100 3 U LH100IR U LH100L 3 100W halogen lamp housings Cable length U LH100 3 290mm Weight 880g U LH100IR 290mm U LH100L 3 800mm Accepted lamp 12V100WHAL high intensity lamp 12V100WHAL L long life lamp 135 depth dimension for installation External power supply TH4 100 or TH4 200 and power cable UYCP are necessary for 100W halogen lamp housings These items are sold separately For TH4 100 200 installation dimensions refer to the next page Unit mm 26 Lamp housing accessories LAMP HOUSING amp ACCESSORIES Forthe 100W halogen lamp the external power supply TH4 100 200 with an intensity adjustment switch and an ON OFF switch both are located close to the operator s hand are provided All Olympus reflected light illuminators can be used with fiber illumination OLYMPUS TH4 100 14 5 TH4 100 200 External power supply 200 Weight 2 2kg U RCV U RMT Extension cord TH4 HS Hand switch Cable length 2 000mm DF converter for BX URA2 Darkfield light excluding tube which is built into the BX URA2 259 for inst
56. tfield darkfield and polarized light characteristics is perfect for Nomarski DIC and polarized light observations MPLFLN5xBDP MPLFLN50xB DP 4 5 926 45 44 41 45 MPLFLN10xBDP 926 38 5 37 36 5 34 1 45 WD 6 5 4 5 MPLFLN100xB DP 926 45 44 41 MPLFLN20xBDP T 927 5 T 927 5 a a z 32 z 32 Unit mm ee Widefield eyepiece WHN10x Super widefield eyepiece SWH10x HIS ODJECENETERSES Field Number 22 Field Number 26 5 Objective lens Numerical Working distance Focal distance Weight Total Practical field Depth of Total Practical field Depth of magnification Aperture mm f mm g magnifications of view mm focus um magnifications of view mm focus um MPLFLN 5xBDP 0 15 12 0 36 95 5 50 4 4 59 50 5 3 59 MPLFLN 10xBDP 0 25 6 5 18 83 3 100 2 2 18 100 2 7 18 MPLFLN 20xBDP 0 40 3 0 9 88 5 200 11 6 1 200 1 3 6 1 MPLFLN 50xBDP 0 75 1 0 3 6 100 5 500 0 44 1 4 500 0 53 1 4 MPLFLN 100xBDP 0 90 1 0 1 8 101 5 1000 0 22 0 73 1000 0 27 0 73 Screw W26x0 706 15 ee r es R UIS2 OBJ ECTIVE LENSES Long WD M Plan SemiApochromat BD LMPLFLN BD series Long working distance Plan SemiApochromat objective lenses giving high level correction for chromatic aberration Su

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