Olympus FV300 User's Manual
Contents
1. l l Agee HNO MEy prae Ahy c vanie lb wadas Mal l AOTF flexible control of the laser intensity to meet the specific demands optional The laser exposure will be limited within the scanning area by default minimizing unnecessary bleaching of the specimen Option includes Any laser intensity for any excitation area Region of Excitation mode Multiple laser applications AOTF controller that provides easy link with external equipment Easy exchange between display methods Independent navigation bars for each image window enable the display method to be changed quickly and easily Tiling display function for see at a glance comparison of multiple images The FV300 s live tiling function which is especially valuable in time course experiments allows observations of multiple images or changes in the specimen while the experiment is in progress Images in series e g XYt or XY Z can be freely displayed Scanning unit set up monitor display Laser Unit ve NN a rg N Image tool bar X Y Z scanning operations and time lapse observations both produce multiple images which can be displayed in sequence simply by clicking the sequential mode button Channel selection and image zooming are also available on the same menu CAMs i o W AATE cn FLUO TF Locked f Thumbnail display Data stored in the gallery window are displayed as thumbnails for2. Concentration Stream C Ratio Background Conc 5 T Set Fmin Set Fmax PR from ROI from RI Ca Kd In Compute Kd Intensify F Mask 1 X Display Image stream ETIEMPO IAGESMNDOT 2 TIF Dual wavelength analysis Accept Result Input output of external trigger signal The optional time course software gives control over the input output trigger signal by GUI It is suitable for combined experiments such as those involving patch clamping PAPP for FRAP Application PAPP Programmable Acquisition Protocol Processor Easy reliable flow of experiments for fluorescence recovery after photobleaching AOTF and PAPP function for effective FRAP Fluorescence Recovery After Photobleaching Fluorescence recovery after photobleaching can be analyzed on any designated area by means of the AOTF equipped laser combiner During the processes of photobleaching and recovery the PAPP function enables time scales to be freely and easily programmed to suit different experiment purposes Mouse hippocampal neurons fluorescence of GFP Living neurons expressing GFP were maintained in culture and fluorescent images were obtained Subsequently FRAP analysis was performed on the same cell to determine the diffusion rate of GFP proteins into the dendritic spines Rapid fluorescence recovery within seconds was observed Shigeo Okabe Department of Anatomy and Cell Biology Tokyo M e
3. slice from the 15th day embryo and observing the living nerve stem Dr Hidenori Tabata Repeat 1 cell and nerve cells subsequently born from it School of Medicine Keio University Registration ajel Add Pos fe ae Eran 2 3 4 5 E 7 8 3 Z Nol Ss Features 1 Measure up to 254 points A variety of scan conditions can be set for each point such as XYZ coordinates the Z axis acquisition range and the detector sensitivity 2 Up to 5 X 5 adjacent fields of view registered automatically I 7 x Since adjacent fields of view are registered automatically it is possible to broaden any given field e while maintaining a high magnification level 3 High precision XY stage scanning ms wide area can be observed with highly precise position No3 reproduction Errors from repetition are not accumulated High precision XY stage complies r exclusively with the PROSCAN model from PRIOR No6 Scientific 4 Flexible scanning conditions The system combines scan flexibility with time lapse imaging for monitoring changes in the specimen over Se time Z Using multi point software it is possible to acquire an XYt an XYZ or and XYZt image series at multiple positions through automated software control of the motorized XY stage Multi point software and motorized XY stage are optional 8 3D Imaging Using multiple 3D images to obtain accurate 3D structure analy
4. Fluoview application software Image acquisition Scanning condition setting image size scanning speed zoom panning etc Real time image calculation Kalman filtering peak integration Hardware control Laser scanning unit microscope Image display Each image display Single channel side by side merge cropping tiling series Z T pass and continuous LUT Individual color setting pseudo color Overlay Lines text scale bar etc Image processing Individual filter Average Low pass High pass Sobel Median Prewitt 2D Laplacian edge enhancement etc Calculations Inter image mathematical and logical DIC back ground leveling Image analysis Overview of fluorescence intensity within an area histogram perimeter measurement for user assigned area time lapse measurement etc 3D visualization 3D animation left right stereo pairs red green stereoscopic images and cross section Others Graphic based help PAPP Programmable Acquisition P rotocol Processor time course software optional trigger IN OUT function optional Multi point software optional Power consumption Objectives for BX and IX using U UCD8 IX LWUCDA and U DICTS Microscope 115V 6A 230V 3A scanning unit PSU 115V 3 5A 230V 2A computer amp monitor 115V 4 5A 230V 104A Ar laser 115V 10A 230V 5A Mult linei Ar laser 115V 10A 230V 5A Kr laser 230V 204 HeNe laser each 115V 0 4A 230V 0
5. by histamine then inhibited by cyproheptadine Cameleon genes provided by Dr Miyawaki Atsushi in Brain Research Institute Equipment FV300 and HeCd laser Time period 4 seconds CFP Fluorescence wavelength 485nm YFP Fluorescence wavelength 530nm Measurement CFP YFP FRET Calcium ion concentration in a live HeLa cell using a cameleon split type indicator Energy transfer between CFP and YFP is proportional to bound calcium The time series shows the increase of calcium ion density caused by stimulation of histamine and the effect of blocking by proheputajin The 440nm diode laser can be added for CFP YFP FRET imaging A 440nm diode laser is optionally available for CFP YFP imaging The 440nm laser line ideally excites CFP with minimal disturbance to YFP and is therefore suitable for CFP YFP FRET experiments The high performance LSM objectives PLAPO40XWLSM and PLAPO60XWLSM are precisely corrected in this wavelength range and ensure the highest measuring reliability For simultaneous observation of CFP and YFP 440nm and 515nm laser lines are required Ratio imaging to analyze 2 wavelength images Using time course software the ratio image can be continuously displayed in pseudo color At the same time the intensity of each channel can be monitored graphically The analysis process is presented as an intuitive flow chart optional time course software TIEMPO View Processor Disabled Image
6. 2 x 512 0 25s Simultaneous scanning of up to 2 channels Selectable from 2 channel fluorescence or 2 channel fluorescence 1 channel transmitted light 3 channel fluorescence using virtual channel Manual selection XY XYZ XYT XYZT XZ XT XZT point Line t free line t line z free line z Clip Zoomin 12 bit 24096 grey levels 1X 10X Step motor Minimum step 10nm BX61 BX61WI and IX81 combination 25nm other microscope combination BX51 BX61 BX51WI BX61WI Inverted special laser safe frame IX81FVSF IX71FVSF side port External trans mitted light unit Transmitted light illumination unit External halogen light source connected to microscope via fiber cable Transmitted light detector External detector unit with built in photomultiplier Connected to microscope frame via fiber cable Fluorescence illumination unit Connect to external mercury light source and microscope via fiber cable Standard equipment of FV300 B X51 FV 300 BX61 FV300 BX51WI FV300 BX61WI PC with system control boards PC AT compatible machine OS Windows XP English version 1GB memory can be expanded to a maximum of 4GB CPU Pentium 4 over 2 8GHz Special I F board image capture PCI bus Graphic board G450 Dual 32MB Hard disk 80GB 7200rpm ID ATA100 with DVD ROM Monitor Two 19 LCD monitors are recommended each able to display 1280x1024 images in full color 16 77 million colors LAN On board
7. 2A LD laser 405nm 440nm 100V 0 9A 230V 0 5A Objectives for fixed stage upright microscopes using WI UCD WI DICTHRA Con che Condenser for BX Condenser for IX U DICTS Objective NA W D DIC Revolving Description NA W D 9 Immersion Correction ring U UCD8A IX LWUCDA prism nosepiece thickness ale M t position Optical element optical elemen MPL5X 0 10 19 60 WI SSNP UMPLFLIOXW 0 30 3 30 U LDPW10H WI SSNP UPLSAPO 10X 0 40 3 1 0 17 U DIC10 IX2 DIC10 normal WI SRE2 UPLAPO 10X03 0 40 0 24 0 17 Cil U DIC10 IX2 DIC 10 normal UMPLFL20XW 0 50 3 30 U LDPW20H WI SSNP WI SRE2 UPLAPO 10XW3 U DIC10 IX2 DIC 10 normal LUMPLFL40XW 0 80 3 30 U LDPW40H WI SSNP UPLSAPO 20X U DIC20 IX2 DIC20 normal WI SRE2 UPLAPO 20X03 0 80 0 19 Oil U DIC20 IX2 DIC20 normal LUM PLFL60XW 0 90 2 00 U LDPW60H WI SSNP WI SRE2 UPLSAPO 40X 0 90 0 2 0 11 0 23 O U DIC 40 IX2 DIC 40 normal LUMPLFLAOXWIR 0 80 330 U LDPWAOH WESSNP UPLFLN 40XO 1 30 0 2 0 17 Oil U DIC40 IX2 DIC40 normal WI SRE2 mom m BEP LUMPLFL6OXW IR 0 90 2 00 U LDPW60H WI SSNP WI SRE2 UPLSAPO 60X0 U DIC60 X2 DIC 60 normal LUMPLFL100XW 1 00 1 50 U LDPW60H WI SSNP WI SRE2 UPLSAPO 60XW U DIC60 X2 DIC60 normal XLUM PLFL20XW 0 95 2 00 U LDPXLU20 WI SNPXLU UPLSAPO 100X0 1 40 0 12 0 17 Oil U DIC100 X2 DIC100 normal HR 14 Note These conditions are not met in confocal microscopy FV300 BX dimensions unit mm FV300 IX dimensions u
8. CONFOCAL LASER SCANNING BIOLOGICAL MICROSCOPES FV300 FLUOVIEW UIS2 World leading optics FV300 CONFOCAL LASER SCANNING MICROSCO PERFORMANCE FOR THE PERSONAL USER The FV300 is the ideal choice of laser scanning microscopes for personal users Its optical system is fully integrated from scanner to microscope and not only delivers outstanding optical sectioning but also ensures the easy flexible expandability required for any future upgrade With its wide choice of options and configurations including the Olympus inverted upright and fixed stage upright microscope platforms the FV300 offers excellent versatility as well as top class laser scanning performance Highest image quality 12 bit 2048x2048 pixel resolution with economical cost Easy operation with user friendly software Simultaneous capture of 2 fluorescence and 1 transmitted light detector images e Capable of the most demanding tasks with a direct and efficient optical system e Optical system chromatically corrects aberrations from UV to NIR near infrared red spectrum Fiber illumination system separates fluorescence and transmitted light sources from the microscope body for improved temperature stability FV300 IX71configuration Q Optical fiber for laser introduction O Beam collimator Polarizer Dichromatic mirror Q Excitation dichromatic mirror Q XY galvanometer mirror scanners Q Pupil lens Q Collector Lens
9. Pinhole turret Emission beam splitter slider D Barrier filter slider D Photo multiplier Software Graphical User Interface Ultimate ease of operation and monitor display Dye selection display When a fluorescence dye is chosen the laser and light path settings are selected automatically with each of the selected fluorescence dyes displayed graphically on the monitor Selected Dyes Available DyesL Mito Tracker Fluo3 GFP uv Hoechst33258 Indo 1 Mito Tracker PI Rhodamine Phalloidin Texas Red TOTO3 TRITC Prev Clear Apply Innovative scanning method for improved performance er OXT Oxvz OXVZT r Mode Surface XY Horm zt O 3 Clip foomin Seq Hormel Fast Line T Horm C Depth XZ Point Size Ar Eur T Zoomin scanning Zoom scanning can be conducted over any designated rectangular area Since only the region of the targeted zoomed in area is acquired scan time and laser exposure of the specimen is minimized Spot Zoomin Point scanning the point scan enables accurate quantitation of physiological events Line scanning SlantLine plane This fast scanning option permits accurate quantitation of physiological events such as Calcium waves or sparks The ultimate in fast scanning intensity changes during rapid A single line may be scanned oriented at any angle in the XY Versatile
10. Setting High Contrast Zoom Mon Max Summary FITC v Temas Red Mode Cumert sce Samples processed 480 000 Being 512 bru per wet 9 intensities per bel Mar samples m any bn 149 708 Areal avons Mode None MirrM ax founds Thed Regent Threshok FITC 11244 Texas Hed 1208 EN Theeihold Annota n Ios sanga nl F 480 LEE BB FITC Thresh 1544 Pl lt theechokd 478 508 83 71 of al comedies E threshold 1 292 D 297 of af samples EM Texas Red Thvosty 1206 arcet Wo ROl selected Mide Form Thresholds Mode Eicolocaliration Processor Eal Dala Seinchon Aniyan Mode Disabisd s Histogram Current shoe Arrototions au RM citreshot 473 705 88 69X of a samples y o Tiu AU 3 008 4200 btentiy eo LUT Setting High Contrast Zoo Mn Max 05 n 37 Summae FITC v Texas Red Mode Lurert tice S argin peocersed 450 000 ww 512 bina per ait O anderen per bn Maw samples in ary bec 149 708 Arranon Mode None Man Max Bound Threshodhs Regon Or JOO M Fegont Aereoraon Tt Total samples e hectoguam 490 000 _ RGB 255 258 245 n Samples node regen 2142 lU 454 n Samples aisde regen 477 A 98 FX Regions Min Max Bound Mode Hide Form Colocalization By using this function to analyze multi color specimens it is possible to discover whether different labeled substances are present in the same region The ability to quantify the P earson correlation the overlapping coefficient and the col
11. Size Y Size Speed Ar Pan Status X Pan Y Pan Angle Sub Mode Normal Normal E M ulti point time lapse system High magnification multi point time lapse observation of living cells Wide ranging specimen observations for improved experiment throughput Use of a motorized XY stage allows the analysis of time lapse changes in many points scattered over a wide area The system is therefore effective for work with thick specimens such as observing changes in the states and movements of stem cells using a brain slice or analyzing expression mechanisms at the individual level in an embryo In wide ranging tasks such as analyzing cell functions using GFP the system provides many different kinds of data at the same time enabling a higher overall level of experiment efficiency even in long lasting observations In addition using separate chambers at the same time makes it possible to perform different experiments at the same time These are just some of the ways in which this system dramatically improves the throughput of experiments requiring long duration observations fej Untitled XY5tage PI Ix Fie Edit View Options Control Cunert Fos um ni 41 an LACER Step Size um je xpo elie M TIEMPO Introduction of DsRed2 expression vector into brain of 14th day Image courtesy of mouse embryo through womb electroporation Taking a specimen Dr Kazunori Nakajima Interval 372 sec x
12. acquisition For the high speed observation ofthe sample Fluoview is capable of scanning 4 frames per second in a fast scanning mode at an image size of 512X512 By limiting the image size the frame rate will be even faster This scanning mode is suitable for living cell observation Versatile line scanning modes have many uses E XE ERIT rp ay E Le r j ka TIT d lle e r DAD MT e Lame erra mt Hy ur E ht eee Ue line i ae ee o ee Pare pede M Tp m c m 4 y v i t A d 2 1 4 s a i Pm Et FAURE nef i x Ite MORE AL TC er EE AMOR re teg SA PIT rara en Ti aM ps J J a5 Ra ijeki oae E m E u F uU pes Le eng Rn PT Ja p ET T H mies Lay m R0 The wide variety of the line scanning modes E i linear slant tree line enables flexible Calcium wave in isolated cardiac myocyte Calcium sparks in isolated cardiac myocyte analysis of rapid time lapse experiments Dr Sandor Gyorke Dr Sandor Gyorke Texas Technical University Texas Technical University Superior slice patching system In combination with the unique fixed stage amp nosepiece focusing BX61WI microscope the FV300 provides a highly effective system for slice patching This unique set up has a small footprint for increased room in a space limited cage The remote control microscope options minimize the danger of LA Na accidentally touching the delicate mr HECTORE experimental settings Olympus also offers Pa
13. bacco hybrid plant cells Dr Wataru Marubashi Laboratory of Plant Breeding and Cell Engineering School of Agriculture Ibaraki University 12 Fluorescence Dyes and Filters BA650RIF BA625IF BA660IF o ASS GS gov eg o 13 Short Pass Band Pass Long Pass SDM Fluorochrome Specifications Laser light source Scanning unit Microscopes Item Visible light laser source Specifications Select from the following laser to mounted on laser combiner Multi line Ar laser 458nm 488nm 515nm Total 40mW Ar laser 488nm 10mW Kr laser 568nm 10mW HeNe G laser 543nm 1mW HeNe R laser 633nm 10mW LD405 405nm 25mW LD440 440nm 5 3mW Laser combiner Scanning method Field number Pinhole Each laser light path is equipped with a continuously variable neutral density filter or AOTF All laser lines are combined to apsis along the same fiber optic Galvanometer mirror scanners both X and Y 20 10 with use of LD405 laser 5 position pinhole turret Image memory and scanning speed Image channel Selection of filters according to staining Scanning modes Image depth resolution Zoom Z drive Upright Standard scanning mode 256 x 256 0 45s 2048 x 2048 10 8355 Bi directional high speed scanning mode 51
14. dical and Dental University New PAPP Programmable Acquisition HART Protocol Processor makes it easy to as program a wide range of experiments Command Frame Using the new PAPP function which is included in standard software the experiment protocol is created by describing the individual steps or phases within the experiment Users can specify detailed conditions and parameters for each step This function enables users to construct complex experimental protocols with minimal effort PAPP is suitable for example for FRAP experiments that require more flexibility Series r Track Image Total Frame 410 Total e 0 8 21 For bm Set to FY Get FY Status Track Start by No v4 v5 6 7 Total S Mode 5 XYT Next Normal 30 Normal Frame 410 Time 0 8 21 56 Normal i 1 To 10 Normal 1 2 3 Normal ro NR 256 ort 0 7 4 j Remaining Time CE A je U 44 2 rame Condition Interval Interval 0 1 40 0 1 40 Image Size Zoom 512 by 512 512 by 512 Q Fluorescence Baseline 28 9 Photobleaching at 0 22 sec interval 0 9 Fluorescence recovery at 0 32 sec interval Fluorescence quantitation Line graph depicting average fluorescence intensity versus time 1 5 x File Comment sd Image File Name DAFLUOVIEWMmages Path SS 0 9 3k oU EE i2 332 Made T H Scan Status Scan X Pos Y Pos X
15. display options Exchange between condensed and full image display modes can be performed with a single touch Individual panel layouts can be changed at will and the panel in use can be placed in any desired position Simplified toolbar A newly designed toolbar with various dedicated buttons has greatly improved ease of use The user can execute a succession of selected processes with one click operation for each Flexible setting of scanning size zoom movement and rotation The observation field and scanning area are both displayed graphically Settings can be confirmed while scrolling through the zoom ratios The pan button lets the operator move the image acquisition area at will and rotation scanning of images is also possible PLAPO ALD Use C A 2 Scan Speed Fast Slow 0 153 Scan Free line scanning Intensity changes may be AS of time along the length of a FreeLine freely drawn line such as the trace of an axon or along a cellular junction Clip scanning By cropping the image selected areas can be cut out of complex image stacks measured over a given period r Preset the conditions for image acquisition and loading Storage of Acquisition Settings enables immediate one touch recall of all the relevant experimental settings and conditions Adding new conditions or altering existing ones is quick and easy
16. easy viewing Single monitor display is also possible Sequential scanning to prevent cross talk Sequential scanning may be used to minimize the fluorescence cross talk often seen between channels in multicolored samples This is achieved by exciting each fluorochrome independently one dye at a time With the AOTF function line sequential scanning is available as well Once optimized steps can be performed easily Excited by only HeNe633 Excited by only Kr568 Y Excited by only Ar488 Composition Supplemental Lasers N Mto standby CL Manual Sean Unit bases ent m p DMiNUSETI SDMS U NS z gt tenson UM Confocal Apm tin e BAS0S 575 BAS6SIE Barrie Petters TD Unit pem Transmereg Oye Lo Human colon crypt Nuclei Blue TO PRO 3 Actin Green Alexa 488 APC gene product Red Alexa 568 Christine Anderson Laboratory of Prof Ray White Hunstsman Cancer Institute Utah T ime Course Using different scanning modes to chart time lapse changes efficiently FreeLine t mmm Calcium wave in Xenopus oocyte Calcium Green staining fluorescence pseudo colored fluorescence image after injection of inositol 3 trisphospate ie tata ia Dar J apan Science and Technology Corporation Exploratory Research for Advanced Technology Zoomin t Mikoshiba cell control project Prof Aya Muto XY Zt Zoomin Z t High speed 4 frames sec image
17. ins Marine Station Stanford University Connecting two PCs by LAN is required gt ade a a as ung re nn ett ue I 4 gt Axon u Clampex 10 Applications Gallery Lucifer Yellow retina ganglion cell TexasRed dopamine operated amacrine cell Prof Shigetada Nakanishi Dept of Biological Sciences Kyoto Univ Faculty of Medicine Fem i T Pu 3 der E Me NO LE A Purkinje cell in the rat cerebellum FITC vesicular GABA transporter VGAT Cy3 vesicular glutamate transporter VGLUT1 Pr Masahiko Watanabe x Ji Mouse hippocampal neurons GFP postsynaptic density protein Rhodamine phalloidin actin Hippocampal neurons expressing a GFP tagged postsynaptic density protein were fixed and stained with rhodamine phalloidin to visualize the localization of cytoplasmic actin filaments In dendrites actin filaments are concentrated in the postsynaptic sites Shigeo Okabe gt enam Department of Anatomy and Cell Biology Lucifer yellow injected visual interneurons of Tokyo Medical and Dental University swallowtail butterfly Extended focus is used for every 100um on 383m Z range image and displayed by overlapping pseudo colors Mituyo Kinoshita Pr Kentaro Arikawa Laboratory of Neuroethology Graduate School of Integrated Science Yokohama City University Department of Anatomy Hokkaido Unive
18. nit mm 4 n j cusam ow B 1x1 mmm wc aha mdi MCN d I x eu x a in o m o E a E E 1130 1200 1130 1200 D Depth 990 PEER Depth 990 Different types of laser combiners External transmitted light detector and fluorescence illumination system Selectable from ND filter or AOTF combiner The shutters and light intensity can be controlled via the Fluoview computer Laser combiner for AOTF is required for multi line Argon laser LD440 laser unit External transmitted light detector system H um Laser combiner with Ar HeNe Red Green lasers Laser combiner with LD405 laser unit Fluorescence illumination system Multi Ar HeNe Red Green lasers Direct fiber connection to scan unit Standard configuration for FV300 BX51 FV300 B X61 Installation stand is not included in the unit FV300 BX51WI FV300 BX61WIl combination Please consult your Olympus dealer for additional laser combinations C gt ISO 9001 v ISO 14001 Certific ation Certific ation Design and production at the OLYMPUS montc CORPORATION Ina Plant conforms with 15014001 specifications for environmental management systems J All brands are trademarks or registered trademarks of their respective owners Monitor images are simulated This product corresponds to regulated goods as stipulated in the Foreign Exchange and Foreign Trade Control Law An exp
19. ocalization index allows colocalization volumes to be compared between different specimens Images can also be analyzed in series Colocalization image white Threshold Mode Threshold lines can be interactively altered Regions Min Max Mode Setting the ROI region of interest on the histogram makes it possible to create a colocalization image Values can also be obtained for Pearson correlation overlapping coefficient and colocalization index Physiolink Analyzing the state of a cell interior by synchronizing electrophysiological and confocal image data Optional software m FILE 5 0 wu rien Mouse Winds hep T m MERGNE LGEua cies Link with patch clamping data e With PCs linked through a LAN Physiolink u EN um can synchronize electrophysiological and REY confocal image data simultaneously With the same time stamp recorded in the two PCs it is possible to access and analyze an image and its patch clamping data corresponding to the Physiolink software time scale The patch clamping graph and Physiolink software are interlocked and activated concurrently Physiolink software complies with the FV300 s high speed scanning enabling msec analysis Ethernet Physiolink software FV300 side t Physioview software Axon PC side c Working conditions Clampex Ver 8 0 or later Pacemaker neuron Sea slug nudibranch Dr Stuart Thompson Department of Biological Sciences Hopk
20. ort license from the J apanese government is required when exporting or leaving J apan with this product Design and production adheres to 1509001 international quality standard Specifications are subject to change without any obligation on the part of the manufacturer Ol YM PU S OLYMPUS CORPORATION OLYMPUS AUSTRALIA PTY LTD Shinjuku Monolith 3 1 Nishi Shinjuku 2 chome Shinjuku ku Tokyo Japan 31 Gilby Road Mt Waverley VIC 3149 Melbourne Australia OLYMPUS EUROPA GMBH OLYMPUS LATIN AMERICA INC Postfach 10 49 08 20034 Hamburg Germany 6100 Blue Lagoon Drive Suite 390 Miami FL 33126 2087 U S A OLYMPUS AMERICA INC Two Corporate Center Drive Melville NY 11747 3157 U S A OLYMPUS SINGAPORE PTE LTD E 491B River Valley Road 12 01 04 Valley Point Office Tower Singapore 248373 SE This catalog is printed by enviromentally friendly OLYMPUS UK LTD De I SOY INK waterless printing system with soy ink WWW olym pu s com 2 8 Honduras Street London EC1Y OTX United Kingdom H Printed in Japan M1444E 0205B
21. rsity School of Medicine Rat tongue taste bud DAPI Nuclei FITC TrkB high affinity receptor for brain derived neurotrophic factor Texas Red Protein Gene Products Pr Shigeru Takami Department of Anatomy School of Health Science Kyorin University Structure of PtK2 cell Human Colon Crypt Nucleus DAPI Blue Alexa 488 and To Pro 3 Actin FITC Green Christine Anderson Prof Ray White s Laboratory Mitochondria Mito Tracker Red Huntsman Cancer Institute U Utah Microtubules Cy5 White 11 ni E K anii X C elegans expressing beta integrin fused to GFP Dr Xioping Xhu and Dr J ohn Plenefisch University of Toledo Dept of Biology Coexpression of EGFP and DsRed in a zebrafish embryo Extended focus image of 10umx28 slice Pr Yasuhiro Kamei Pr Shunsuke Yuba Institute for Molecular and Cellular Biology Osaka University GFP labeling of Drosophila adult brain with staining of mushroom bodies Assistant Prof Aigaki Cytogenetics Tokyo Metropolitan University Science Dept Expression of DsRed in a zebrafish embryo Extended focus image of 5umx30 slice Pr Yasuhiro Kamei Pr Shunsuke Yuba Institute for Molecular and Cellular Biology Osaka University GFP labeling of Drosophila adults Plant s amp Isolated Zinnia mesophyll cells Keisuke Obara Pr Hiroo Fukuda Department of Biological Sciences Graduate School of Science The University of Tokyo Apoptosis of Ta
22. sis es X Y Z Y Zoomin Z X Z Y Z Easy Z axis operation and setting The upper and lower limit of Z scanning can be specified interactively by actually scanning the sample or by direct input of the numerical value Z Stop SlantLine Z FreeLine Z 6 0 pim ee Go Set AR a Current Pos 6 0 um Set Zero 1 Satz 0 16 pm Go Sel Step Size Slices 014um 45 Ideal 1 1 0 414 um i Locked z TUT ELIE h Acquire X Y Z images and display X Y cross sectional images quickly and continuously in increments of 0 01 um Thanks to the precision driving mechanism that enables 0 01um step control within the BX61 BX61WI and IX81 motorized microscopes high quality continuous cross sectional images can be acquired The 3D function also provides extended focus projections red green stereo views topographic projections and 3D animations for exploring the structure of the sample Multi plane images can be created from an XYZ image series enabling easy measurement and observation of horizontal and vertical cross sections Other useful procedures include 3D image cropping series animation and simple volume measurement 0 025um is the smallest increment for other microscope combinations ee Topographic projection Height of 3D structure indicated by color C olocalization Analyzing the degree of intensity overlap between channels Image Mesures X1 _ Fite LUT
23. ten clamp ideal non cover glass long working distance water immersion objectives and an optional XY translation stage that moves the entire confocal microscope system while the sample and other experimental hardware remains in a fixed position ROI designation Intensity versus time measurement Immersion type LUMPLFL objectives The 40X water immersion objective in this series has a 3 3mm working distance and an extremely fine tip which is suitable for micromanipulation using a fixed stage upright microscope It has a large N A 0 8 and is also ideal for confocal observations When using the BX61WI fixed stage amp nosepiece focusing upright microscope with water immersion objectives confocal imaging can be used to monitor time lapse fluorescence changes in thick specimens such as brain slices Highly precise time lapse analysis Fluoview s wide dynamic range of 12 bit or 4096 grey levels provides enough sensitivity to detect even the slightest changes in intensity The user can designate multiple regions of interest ROI by using drawing tools The fluorescence intensity or the ratio can be analyzed with the intuitive GUI driven program Long working distance non cover BX61WI fixed stage upright microscope translation stage glass water immersion objective 5 FRET Hardware and software support to optimize the environment for FRET Ratio changes when cameleon is manifested on the HeLa cell and stimulated
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