Olympus AX70 User's Manual
Contents
1. ISO 14001 Design and production adheres to 509001 international quality standard Accredited by the Dutch Council for Accreditation ISO 9001 OLYMPUS OPTICAL CO U K LTD 2 8 Honduras Street London EC1Y OTX United Kingdom OLYMPUS AUSTRALIA PT Y LTD 31 Gilby Road Mt Waverley VIC 3149 Melbourne Australia OLYMPUS LATIN AMERICA INC 6100 Blue Lagoon Drive Suite 390 Miami FL 33126 2087 U S A 491B River Valley Road 12 01 04 Valley Point Office Tower Singapore 248373 ISO 14001 Certification UKAS Design and production at the Olympus EM ANAGEMENT Optical Co Ltd Ina Plant conforms with 15014001 specifications for environmental management systems2. RESEARCH SYSTEM MICROSCOPE AX70 PROVIS LIS UNIVERSAL INFINITY SYSTEM Essential research equipment WE Thereferen for future m AUTOMATIC OPERATION WORLD CLASS OPTICS ILLUMINATION VERSATILITY LJ HIGH STABILITY Co USER COMFORT on The dramatic progress in biological and medical research demands that microscopes do more than answer today s EU needs To be viable they must also offer the performance potential and system flexibility to accommodate tomorrow s research needs To date only one microscope fulfills all these demands the AX70 Research Microscope from Olympus Offering uncompromising performance and outstanding system integration potential the AX70 is a reference standard for future microscopes PROVIS stands for PROfessional VISion Ze standard Croscopes AX70 and it s Universal Photo System the ultimate combination for fully automatic photomicrography The AX70 and its automatic photomicrography system represent P the ideal combination for scientific applications Using the AX70 together with the U PHOTO Universal Photo System provides a very advanced level of microscope performance and sophisticated photomicrography capabilities Combining the mainframe and its corresponding auto focus function with the U AFPHOTO and motorized units integrates innovative technology with a system that fully supports automated photomi
3. applications AX70 the obvious choice The AX70 accepts a high sensitivity camera and high 2D resolution camera simultaneously using a multi port head and offers superior stability for mounting a video camera VEC objectives and Nomarski DIC prisms are also provided to deliver brightly illuminated higher contrast images Maximum operator comfort at all times An extra wide stage ideal for experiments and observation of small animals An extra large 6 stage offers the ideal design for examining mice and other large specimens The AX70 also has enough space between the stage surface and arm to easily accommodate large and thick specimens Improved operational ease for enhanced manipulation The revolutionary Y shape of the AX70 frame offers unique user benefits It COMFORTABLE OPERATION The thrombus model in microcirculation of the rat mesentery LCPL 20x Photo magnification 5x brightfield not only allows the operator s forearms to be supported by the worktable or bench surface but also provides more working space As a result micromanipulators can be conveniently mounted A multi control box can also be mounted for easy system upgrades along with other peripheral equipment The unique design allows arms and wrists to remain on the work surface while manipulating controls Thus efficient observation with less fatigue even over prolonged periods is possible Application e
4. OCA effectively eliminates the deviations during multiple exposure 20 Powerful partners provide solutions to specific application requirements U SWTR 2 super widefield trinocular tube U SWETR super widefield erect image trinocular tube Observation tubes The AX Series observation tubes range from widefield binocular to super widefield erect image trinocular corresponding to various observation needs 21 ACCESSORIES U MPH multi port head The U MPH enables erect image PM30 automatic photomicrography system The exclusive Super FL Auto mode provides fully automatic exposure control for effortless fluorescence photo micrography The Auto mode accommodates all other observation methods and a host of other convenience features facilitate superior trouble free photomicrography observation of a super widefield of view and makes simplified micromanipulation on the upright microscope possible Centering rotation magnification and focus adjustments are also possible for the second image port As a result images from video cameras mounted on ports 1 and 2 offer perfect registration eOptional adapters allow multichannel measurement for up to five ports eOptical devices such as dichroic mirrors attached to ports 1 and 2 can be changed via the light path selector Video camera adapters The AX trinocular tubes accept a variety of video camera adapters for various image systems such as i
5. System features a flexible spot measurement function which allows the 0 196 micro spot and 196 spot measurement areas to be moved without restriction to any desired location This function is invaluable when a top quality photomicrograph is required for instance of multi stained fluorescent samples Rat kidney triple stain UBG Super FL Auto mode for automatic exposure control The Super FL Auto mode is specifically designed for fluorescence photo micrography It automatically selects and measures only the fluorescing image elements while automatically accounting for their field coverage This mode enables operators of any experience level to take high quality photo micrographs This can be performed without making exposure corrections Two other automatic exposure control modes are also available the FL Auto and Auto modes depending on sample conditions Zoom magnification change for versatile framing A1x 2X zoom magnification change system significantly eases zooming and trimming of a desired section to significantly increase framing options flexibility for video recording Cai MPU 3 way camera light path A built in 3 way light path enables simpli fied photomicrography using monochro matic color and large 2D format cameras without camera replacement Bright frame function for easy framing against a dark background The frame control function allows easy framing against a da
6. a high numerical aperture the UAPO 340 Series provides the maximum performance in fluorescence microscopy through near ultraviolet excitation including Ca photometry UPLAPO 60x WPSF Series Outstanding optical performance combines with high numerical aperture and long working distance to make these Universal Apochromat water immersion objectives ideal for 3D observations with a confocal laser scanning microscope With improved near ultraviolet transmission these objectives can also be used for Ca photometry UAPO W Series The UAPO W water immersion objectives feature a high numerical aperture Thus they enable the production of aberration free images at every specimen level from the surface down to deep sections during examination of living cells or other specimens with a refractive index similar to water A y N ue il A Fs ry os ea y s N O D c3 Q im C C c3 gt gt C Q OQ n Q uf OQ E N gt Q y a C3 gt Maximum quality for Bright high quality fluorescence images The AX optical system attains the very best brightness and assures crystal clear fluorescence images by employing an improved numerical aperture and high transmission objectives and by incorporating a 100 optical path and high tra
7. be easily operated via the touch panel while setting the photomicrography conditions and various motorized units The setup choices can be selected via the jog dial 1 Photomicrography conditions setting screen Selection of camera framing photomicrography mode and exposure mode can be made via this screen 3 INIT preference setting screen Selection of motorized units such as objectives and mirror units can be made via this screen 2 Illumination setting screen Selection of on off and changeover of light control field diaphragm AS and excitation light control can be made via this screen 4 Auto focus setting screen In addition to accommodating many microscopic techniques except phase contrast the system s built in real time mode maintains constant specimen focus Use of auto focus with AX70 requires U AFPHOTO AX FOM U MCB and AX PCBT Since modification of AX70 unit is also required please consult your Olympus dealer 18 Fluorescence microscopy dedicated accessories support full research efficiency AX REXBA The use of a dual band filter allows simultaneous observation of double stained specimens With the conventional observation method photomicrography or image documentation with optimally balanced light intensity is difficult since one of the two fluorochromes may emit fluorescence that is too weak or too strong However by simply turning the dial of the AX REXBA easy control of
8. the fluorescence balance is assured for photomicrography with ideally balanced light U S patent pending 1 Use of a dual band filter allows observation of and photomicrography with a double stained fluorescing specimen under optimum conditions regardless of the conditions of the stained specimen 2 As the AX REXBA allows adjustment of excitation light unnecessary irradiation is prevented and damage to the specimen is minimized 3 The 25EBFB G filter accommodates a double stained fluorescing specimen using two types of fluorochromes FITC TRITC and FITC Texas Red This filter is designed for general use with double stained specimens with G B excitation The 25EBFU B filter designed for U B excitation is also available To be released October 1997 4 With single band excitation the AX REXBA can serve as a continuous light control unit When 25EBFB G is used the AX REXBA is applicable for B or G excitation 5 The AX REXBA is easily installed into the AX70 s aperture stop position Observation comparison without and with AX REXBA Human chromosome telomere FITC Pe UPLAPO100x PE3 3x Without using AX REXBA With use of AX REXBA ACCESSORIES Specimens viewed with AX REXBA Rat originated cell culture FITC TRITC UPLAPO40x PE3 3X TRITC enhanced FITC enhanced Precise correction for image deviation in multiple exposure Optical center adjuster AX OCA The AX
9. 00 x When using video camera 2 35mm left 35mm right 100 0 0 100 Photo magnification 35mm camera 2 5x 5x large format camera 7 5x 15x Zoom Motorized control by jog dial or fixed magnification switch of U MCB Zoom ratio 2x Focusing telescope Photo frame reticle m Manual insertion magnification ratio 3 4x besides U AFPHOTO Manual insertion bright frame operation red yellow illumination adjustment seven levels Shutter F Pulse control electromagnetic shutter shutter speed max 1 125 sec lt 2D image analysis memory mode by the CCD 0 1 spot measurement 1 spot measurement 30 Photometry average measurement switchable Flexible spot measurement area spot color switchable red yellow Photometry conditions Five exposure modes Auto mode FL Auto mode Super FL Auto mode Manual mode time mode Auto exposure range 1 125 sec 68min ISO 3 25 600 automatic setting by DX method manual setting also possible 13 step exposure control 0 25 4 every 1 3 step Auto bracketing function eight level reciprocity failure correction multi exposure AE lock exposure time recall data scale imprinting function by imprinting unit Camera back 35mm PM C35DxX auto loading wind rewind and automatic film detection data imprinting unit can be attached automatic setting ofthe ISO value by the DX code Frame counting number is retained for data back up Data imprinting unit P
10. Brace Koehler compensator UPLFL P objectives make high sensitivity polarized light observation possible UPLAPO PH and PLAPO60x PH Series Objectives with the world s highest resolution and contrast for phase contrast observation Designed for phase contrast microscopy these objectives also perform faultlessly with brightfield and fluorescence observations WORLD CLASS OPTICS F N 26 5 for any microscopic technique Super widefield observation tubes enable the AX70 to have an extended field of view F N 26 5 for brightfield fluorescence Nomarski DIC phase contrast and polarized light observations Ideal resolution and contrast In the AX70 s innovative UIS infinity corrected optical system light travels as parallel rays from the objective through the body tube These rays are focused by the telan lens to form a completely aberration free intermediate image This principle allows various attachments to be added between the objective and the built in telan lens without any magnification changes or image deterioration The result is optimum UPLFL PH Series Universal phase contrast objectives that provide the same critical optical performance as the UPLFL Series A newly developed phase membrane allows a high contrast image to be produced without using an interference filter UAPO 340 Series Composed of strictly selected lenses these universal objectives feature high transmission at 340nm With
11. M DI 35 backlit exchange type Large format camera back attached to U DLF Polaroid PM CP 3 Multi control box Operation by transmitted touch panel and jog dial sheet switch Graphics indicated on plasma display Centralized control of mentioned photomicrography conditions and all motorized parts are possible U MCB mn Memory card function can register recall delete print the information of photometry and observation External interface function selectable from RS232C GPIB or printer interface optional Optical path difference based contrast system Operation modes real time mode and one shot mode Auto focus switchable Applicable magnifications 1 25x 100x Applicable condensers AX UCDM U UCDB on U AFPHOTO only U AAC U ULC 2 Applicable microscopic observation brightfield darkfield Nomarski DIC polarized light both transmitted and incident light fluorescence incident light only Eyepiece Objectives Condensers 10x F N 26 5 super widefield of view Conform to ISO 1 PLAPO set PLAPO2x UPLAPO set n UPLAFL set Alf dpt UP LAPO4x PLAPO4x PLAFL4x UPLAPO10x UPLAPO10x UPLAFL10x UPLAPO20x UPLAPO20x UPLAFL20x PLAPO40x UPLAPO40x UPLAFL40x PLAPO100x O UPLAPO100x O UPLAFL100x O Universal motorized condenser for AX AX UCDM N A 0 16 0 9 N A 0 16 1 4 with change of top lenses objectives 1 25 x 100x Turret aperture diaphragm top lens changeover polarizing plate attachment
12. a aberration Such problems occur as the distance between the objective and telan lens is lengthened Olympus solved this problem by developing new eyepieces to make it possible to add two intermediate attachments and assure superb field flatness Now it is simple to integrate the researcher s own units into the microscopic system without additional lenses An impressive line of high performance UIS objectives PLAPO Series Designed for unsurpassed resolution and contrast these new Plan Apochromat objectives keep chromatic aberration down to an absolute minimum Precision manufacturing allows the PLAPO Series to deliver maximum optical performance UPLAPO Series The UPLAPO Series of Universal Plan Apochromat objectives features an unsurpassed high numerical aperture In addition to brightfield observation they offer perfect resolution and contrast during fluorescence Nomarski DIC and darkfield observations UPLFL Series Cost effective Universal Semi Apochromat objectives deliver superb resolution and contrast with any microscopic technique such as brightfield fluorescence Nomarski DIC and darkfield Olympus proprietary manufacturing technology makes possible high precision objectives at affordable cost UPLFL P Series Featuring outstanding optical performance like the UPLFL Series innovative assembling technology has made the UPLFL P Series completely strain free In combination with the
13. crography This combination offers fast auto focus in brightfield darkfield fluorescence and Nomarski Differential Interference Contrast and provides automatic light intensity adjustment for the objective in use as well as automatic observation light path selection Flexible moving spot measurement ensures optimum exposure regardless of specimen a distribution A built in Super FL Auto OLE mode offers fully automatic exposure i control for fluorescence C photomicrography And easy flexible framing is assured via the zoom magnification system By centralizing operation with a multi control box the entire procedure from observation through photomicrography can be effectively automated The U PHOTO 20 80 is also available from Olympus for simultaneous eyepiece and video camera observation The Auto focus can be activated by inserting an optional memory card in fluorescence and Nomarski DIC microscopy AX70 Universal Photo System Effective solutions to research demands from a flexible system geared to fulfill present and future requirements The AX70 starts by offering all the advantages of the proprietary UIS optical system to provide the best image quality possible and adds universal adaptability for rapid changeovers between various microscopic techniques The specially designed Y shape frame assures superb operator comfort A choice of illuminator systems suits a range
14. hment of laser and additional illuminators This versatility permits application specific incident light illumination In particular it enables laser illumination for laser manipulation and caged compound release photoactivation Twin lamp housing configuration with a quick one touch illumination changeover Two lamp housings can be mounted simultaneously onto the AX70 to significantly expand the microscope s potential Infrared IR excitation and ILLUMINATION VERSATILITY Photoactivation of caged fluorescein Caged fluorescein labeled tubulin had been introduced in the cell after exposure to UV light through a slit a photoactivated zone was generated conventional ultraviolet UV excitation are both required when fluorescence microscopy moves toward multi staining However this requires switching the light source from the conventional mercury burner to a halogen source The twin lamp housing configuration on the AX70 makes the switchover between the mercury burner and halogen source a simple operation Quick excitation light switchover a convenient feature for various photometry and measurement applications Working in combination with the OSP100 DNA photometry system the AX70 easily handles different measuring methods using several fluorescent indicators These range from 1 wavelength excitation 1 wavelength photometry 1Ex1Em to 2 wavelength excitation 2 wavelength photometry 2Ex2Em T
15. hus the AX70 OSP100 combination can measure fluorescence intensities at a single wavelength with dual wavelength excitation using the fura 2 or BCECF indicator These advantages make it ideal for analyzing intracellular calcium ion concentrations and pH values Application example PAF Photoactivated Fluorescence The PAF technique irradiates caged compound with an ultraviolet ray instantly transforming them to original active substances The induced reaction is then measured and examined PAF also allows control of intracellular reaction time and location Applications eExamining muscle contraction due to increased intracellular Ca concentrations Study of cytoskeleton assemblies elon channel research eStudy of receptor bonding of neural transmitters and their functions AX70 the obvious choice The AX70 incorporates a vertical illuminator with sideways illumination capability The Y shape also provides an uncluttered work surface for conveniently positioning an injector or other ancillary equipment near the stand An upright microscope is required to observe internal structures of cells with samples usually measuring 100 200um thick 10 structural integrity for vibration resistance weighty accessories and thermal stability HIGH STABILITY NG108 Left prior to enhancement Right VEC DIC Frame stability the basis of Heat resistant light source Vibration resistant rigidi
16. icated Olympus optical design algorithms this ensures unmatched image contrast for polarized light observation Used with the Brace Koehler compensators these objectives allow high sensitivity examination of biological samples Live cell imaging The special correction of the UPLAPO60x WPSF objective renders symmetrical image information from planes above and below the focal plane This imaging symmetry makes it the objective of choice of digital deconvolution applications The water immersion correction of the objective eliminates spherical aberration when imaging live material even when observing deep within a cell up to 250um At the same time the objective s high numerical aperturd N A 1 20 achieves spectacular resolution General objectives Non symmetry of image information from the planes above and below the focus level as obtained with well corrected objectives ZS 7077 LL VY 7AN LENT VW M S SALT ZZ SN PSF objectives The special correction of the UPLAPO60x WPSF objective renders symmetrical image information from planes above and below the focal plane A range of lluminator systems for advanced research applications Field stop and aperture stop units for easy attachment of laser and other special illuminators Removable field stop and aperture stop units facilitate simple attachment and detac
17. inclined 24 magnification direct optical path 1x optical path through zoom 1 2x zoom Two 35mm cameras one large format camera one video camera all can be attached at the same Camera time two video cameras can be mounted by connecting U TVL to the large format port selectable by U MCB eU PHOTO U AFPHOTO Direct optical path Bl video camera direct 100 0 0 100 P eripheral optical path all light goes to camera optical path during exposure Bl video camera direct video camera 2 photometry 100 0 0 0 0 100 0 0 0 0 100 0 20 0 0 80 0 20 0 80 video camera 2 is mounted to the large format port via U TVL Xx When using auto focus function of U AFPHOTO Bl video camera direct video camera 2 photometry AF sensor 50 0 0 0 50 0 50 0 0 50 0 0 50 0 50 10 0 0 80 10 0 10 0 80 10 Camera optical path 35mm left large format 35mm right 100 0 0 0 100 0 0 0 10 0 Optical path changeover When using video camera 2 35mm left 35mm right 2 100 0 0 100 u eU PHOTO20 80 5 Direct optical path 2 Bl video camera direct 100 0 0 100 Q Peripheral optical path 80 of all light goes to camera optical path during exposure E BI video camera direct video camera 2 photometry le 20 0 80 0 100 0 0 0 0 100 0 0 20 0 0 80 0 20 0 80 s video camera 2 is mounted to large format port via U TVL E Camera optical path 35mm left large format 35mm right 100 0 0 0 100 0 0 0 1
18. insertion all motorized Applicable for transmitted brightfield transmitted darkfield transmitted phase contrast transmitted Nomarski DIC transmitted simple polarizing observations Swing out Achromat U SC N A 0 16 0 9 objectives 2 x 100x Achromat Aplanat U AAC N A 1 4 objectives 10 x 100x Universal U UCDB N A 0 2 0 9 objectives 2x 100x Phase contrast darkfield U P CD N A 1 25 objectives 10 x 100x For super low magnification U ULC 2 N A 0 16 objectives 1 25 x 4x Darkfield dry N A 0 8 0 92 objectives 10 x 40x Darkfield oil N A 1 20 1 40 objectives 10x 100x 1 Conform to IS09345 1 1508038 22 AX70 dimensions unit mm Specifications are subject to change without any obligation on the part ofthe manufacturer OLYMPUS www olympus com AX70 Universal Photo System dimensions unit mm 200 BE 470 385 OLYMPUS OPTICAL CO LTD San Ei building 22 2 Nishi Shinjuku 1 chome Shinjuku ku Tokyo Japan OLYMPUS OPTICAL CO EUROPA GMBH Postfach 10 49 08 20034 Hamburg Germany OLYMPUS AMERICA INC 2 Corporate Center Drive Melville NY 11747 3157 U S A OLYMPUS SINGAPORE PTE LTD ISO 9001 Certific ation
19. ivity less than lum Main switch located at power supply U PS light adjustment dial right front field control knob left front sub main switch changeover switch for Operation control position transmitted incident illumination light preset switch right wing of frame hand switch attached objectives direct changeover objectives recall mirror unit direct changeover Incident excitation shutter on off lamp off possible Power supply U PS 100 120 220 230 240 V automatic changeover main switch attached when using U SWTR 2 F N 26 5 super widefield adjustable i interpupillary distance range 50 76mm m Observation tube inclined 24 right eyepiece sleeve correction Integrated in photomicrographic unit mechanism photo focusing can be attached reticle photo mask rotation stop included erect image U SWETR attachable Revolving nosepiece Motorized sextuple nosepiece motorized quintuple nosepiece for reflected darkfield observation various manual nosepieces attachable Stage Stroke range 52x 76mm with vernier max two sliders can be inserted torque adjustable selectable from right handle or left handle knob position tip g 57mm from desk top Condenser holder Vertical stroke range 25mm when using AX UCDM 10mm centering adjustment function AX UCDM standard feature both sides condenser movement control knobs AX UCDM motorized Olsen er loe F N 26 5 super widefield adjustable interpupillary distance range 50 76mm
20. mage processing and video documentation systems 1 U IMAD 2 U SMAD 3 U BMAD 4 U CMAD 5 U TV1X 6 U TVO 5X 7 U PMTV 8 U PMTVIX 9 U PMTVC AX70 specifications Item Type AX70 Y shape frame with built in transmitted incident light vertical illuminator AX70 U PHOTO U PHOTO20 80 U AFPHOTO Optical system UIS optical system infinity corrected compensation free Transmitted illumination 12V100W halogen 2 000 hours long life type pre centered Mechanical field diaphragm motorized field diaphragm unit attachable g Six built in manual filters ND1 5 ND3 ND6 ND12 IF550 LBD motorized filter changer attachable B One optional external filter can be user installed manual operation 9 incident illumination Universal vertical illuminator 12V100W halogen Hg100W Xe75W applicable optical devices detachable motorized mirror unit changer excitation light o shutter interlocking operation when changing an objective observation method Max three filter sliders six filters attachable when using AX RFCH O Illumination adjustment 1 12V adjustable in units of 0 1V by the jog dial light preset switch transmitted incident halogen illumination changeover switch Eocus Vertical stage movement by manual roller guide stage travel range 25mm Coaxial coarse and fine focus handle minimum fine focus adjustment graduation lum increments stroke per rotation 0 1mm fine 15mm coarse Sensit
21. niform fluorescence over a wide field of view encompassing the entire excitation eEfficient heat and IR removal in the collector lens protects interference exciter filters from unnecessary energy eThe automation capability RS232C GPIB of the PROVIS AX70 makes it an ideal base for automated FISH imaging stations 16 17 Sophisticated photo and video documentation systems U PHOTO Universal Photo System The U PHOTO Universal Photo System is an automatic photomicrography system that provides fully automatic functions for photomicrography These include a flexible spot measurement function a Super FL Auto mode specifically designed for fluorescence photomicrography and a zoom magnification changeover The U AFPHOTO system also features an auto focus mechanism capable of accommodating a wide range of microscopic techniques and objective magnifications Each of these versatile functions can be controlled via the multi control box for fully automatic photo documentation The U PHOTO 20 80 for simultaneous eyepiece and video camera observation is also available and is especially effective for use in pathology conferencing For simultaneous observation a video camera mounts onto the large format port via the U TVL Ta ri n ro m r ra Distribution of copper accumulation in a larva of Ephemeroptera Microcirculation of the rat mesentery fluorescein dextran Flexible moving spot measurement The Universal Photo
22. nsmission optics The ideal mirror unit for each application can be selected from a range of 18 The built in illuminator accepts up to four mirror units allowing the user to create the ideal combination for each purpose or for a combination of multiple observation methods In addition the motorized excitation light shutter also minimizes fluorescence fading Impeccable phase contrast observation The AX Series features new phase contrast objectives employing an optimally positioned innovative low reflection phase membrane Appropriate phase contrast and transmission are ensured for a wide wavelength range for outstanding contrast and image quality Nomarski DIC with higher contrast and resolution Sophisticated manufacturing technology and new multi coatings for the high performance Universal Apochromat objectives guarantee superb optical performance for fluorescence and Nomarski DIC observations Nomarski prisms specially developed for Video Enhanced Contrast and metallurgy are also available Design algorithms suited for polarized light observation The entire UIS optical system including high precision strain free POL objectives condensers and observation tube prisms has been designed to minimize reflections from Defocus images of the point spread function RESOLUTION AND CONTRAST IMAGING lens surfaces and frame Together with ded
23. of different microscopic methods And the system s substantial frame accepts heavy attachments for image processing and photometry applications These and many other advanced features boost the performance of the AX70 and ensure flexible system upgradability Olympus further maximizes system versatility with various accessories including the AX REXBA to optimally balance fluorescence for double stained fluorescing specimens The broad range of accessories available ensures that Olympus answers every user need Thanks to these and other significant advantages the PROVIS AX70 system accommodates increasingly complex and multi facetted research and experimental requirements The Olympus UIS optics The new standard of excellence Aberration free image even with added attachments Innovative UIS infinity corrected optics such as objectives telan lenses eyepieces and other lens units completely eliminate optical aberrations In this system light travels as parallel rays from the objective through the body tube These rays are focused by the telan lens to form an aberration free intermediate image This principle allows attachments to be added between the objective and the built in telan lens without any magnification changes or image quality deterioration Simple modification of microscopic systems The major drawback of conventional infinity corrected optical systems is field flatness degradation and com
24. rk background during such observation methods as fluorescence and darkfield The brightness of the red or yellow colored frame can also be changed for smoother faster frame adjustment Fully automatic photomicrography via multi control box The use of the multi control box enables fully automatic photo and video docu mentation Excellent visibility is also offered courtesy of a plasma display while a touch panel keeps operation as simple as possible The multi control box represents maximum operational comfort especially for frequent users of photo micrography or video documentation RESEARCH DOCUMENTATION U AFPHOTO offers auto focus capability for a wide range of microscope techniques The U AFPHOTO is equipped with a high performance auto focus mechanism that accommodates a wide range of objective magnifications 1 25x 100x as well as brightfield darkfield fluorescence and Nomarski DIC observation methods The focusing speed during ordinary brightfield observation has been raised significantly to achieve focus in only 0 3 2 seconds In addition to the one shot mode a real time mode is included to maintain specimen focus and significantly enhance observation efficiency even during objective and microscopic technique changeovers or specimen movement The Auto focus function can be activated by inserting an optional memory card in fluorescence and Nomarski DIC observations The U MCB can
25. ty ensures system flexibility structure the best results for Current biological research The power supply represents a electrophysiological experiments applications require more than a high source of heat for microscopes To The AX70 s tripod like frame design quality microscope They demand a achieve the best long term thermal offers a wider work surface and complete system configuration stability the power supply was reduces installation space including image processor removed from the body of the requirements In addition it provides photometry system and laser source microscope The AX70 was also rock solid stability To enhance system flexibility the given a heat insulating structure to frame must be strong enough to reduce the temperature influence of support the heaviest attachments To the lamp housing on the frame achieve an exceptionally rigid frame These measures effectively minimize for the AX70 Olympus used heat induced frame flexure on the advanced computer simulation AX70 techniques in the design process HE Application example Video Enhanced Contrast A low contrast sample unidentifiable in ordinary microscopic observation can be recorded using a high sensitivity camera and contrast enhancement by means of image processing The result is unprecedented image contrast and sharpness A Nomarski DIC attachment with a greater numerical aperture for higher resolution is optionally available for Video Enhanced Contrast VEC
26. ues automatically The use of a multi control box may automate many functions including photomicrography A computer based control system For more advanced features the multi control box can be linked to an external computer via an interface A computer control system can therefore be configured for other ancillary equipment connected for use in FISH patch clamping and other biological research applications Remote controlled photomicrography operation is also possible FISH and Fluorescence advantages The PROVIS AX70 thoroughly addresses the concerns of Fluorescence and Fluorescence n Situ Hybridization FISH microscopy accuracy emission spectrum eUIS objectives produce fully corrected primary images This leads to exceptional RGB image registration for high resolution CCDs eHigh N A fluorescence optimized Fluorite and Plan Apochromat objectives deliver images of exceptional brilliance contrast and resolution eThe wide choices of filter systems satisfies every fluorescent label and includes filter cubes for simultaneous multi wavelength excitation and emission In addition filter systems can be tailored to future demands Ready adaptability of filter wheels to the massive stand enables the use of stationary multichroic beam splitter emission filters with single dual and triple wavelength exciter This approach renders the highest registration eThe chromatically corrected illumination beampath produces u
27. xample Intravital Microscopy Applications When small laboratory animals such as mice etc need to be microscopically studied several prerequisites must be met Next to suitable optics capable of saline immersion a large work surface is needed to support the specimen as well as tools perfusion equipment etc Study of capillaries microcirculation AX70 the obvious choice The AX70 is ideal for mounting a large 6 stage suitable for support of large specimens 14 Automation facilitates research microscopy 1 AX motorized focusing unit AX FOM 2 AX motorized transmitted field stop unit AX TFSM 3 AX motorized transmitted filter changer AX TFCHM 4 AX motorized universal condenser AX UCDM AUTOMATIC OPERATION Chromosome 1 4 and 9 in human metaphase Chromosomes Spectrum Orange FITC DAPI UPLAPO 100x The combination of motorized units with a multi control box automates the entire microscopic procedure A motorized universal condenser allows quick simplified changeover between different microscopic methods The AX70 also includes a motorized filter changer and motorized revolving nosepiece to speed selection of excitation wavelength In addition the AX70 allows the operator to affect setting changes This is achieved through the motorized field stop and aperture stop units and the motorized filer changeover which can be used to perform various microscopic illumination techniq
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