Nikon COOLPIX 4500 User's Manual
Contents
1. Nikon INSTRUMENTS INC Mounting a Coolpix 4500 onto a Microscope Reference Sheet Please refer to the Coolpix 4500 User s Manual for page numbers indicated below Mounting the Coolpix 4500 to a microscope trinocular photo tube or beamsplitter With the camera power off open the card slot cover and make sure a CompactFlash Card or IBM Microdrive is properly inserted into MDC A Relay Lens the camera See p 12 Carefully thread the MDC A Relay Lens onto the camera s lens chet tbe ES Likewise thread the C mount onto the MDC Relay Lens securely Note that over tightening the adapter may damage threads of both Gs adapter and apparatus Attach the C mount adapter onto the microscope s trinocular or beamsplitter port Secure the C mount into place Swivel the LCD screen to the desired vewing position and angle Note The EH 52 AC Adapter is recommended to eliminate battery consumption Mounting the Coolpix 4500 into a microscope eyepiece tube 1 With the camera power off open the card slot cover and make sure a CompactFlash Card or IBM Microdrive is properly inserted into the camera See p 12 MDC A Relay Lens Remove the cover ring from the relay lens by loosening the three SOOLPIX MDC Lens setscrews aton JAPAN a Attach the MDC A relay lens to the camera lens Coverking Note that over tightening the adapter may damage threads of both Setserew x3 adapter and appa2. hy l White Balance The default setting is Auto Select a white balance setting that matches the microscope light source incandescent for tungsten or halogen illumination Select White Bal Preset for unusual lighting conditions See p 92 Metering The default setting is Matrix Setting it to Spot on a targeted sample with a very dark or a very bright background would ensure most appropriate exposure See p 95 Continuous The default setting is S single shutter release Using other settings continuous sequence of pictures may be taken while the shutter release button is held down See p 96 Best Selector Use default setting Off See p 98 Image Adjustment The default setting is Auto Certain illumination techniques such as DIC phase contrast OCC etc may require special brightness and control adjustments for best results See p 99 Saturation Control The Black amp White setting will enhance black and white samples See p 99 100 Image Quality Image quality settings other than H are compressed as they are saved into memory Compression selectively reduces the quality of an image See p 102 Image Sharpening The default setting is Auto See p 105 ar mne Nikon Instruments Inc Nikonusa com 1 800 52 NIKON
3. ratus Remove an eyepiece from the microscope and insert the MDC A adapter as replacement Swivel the LCD screen to the desired vewing position and angle Note The EH 52 AC Adapter is recommended to eliminate battery consumption This reference sheet is to be used in addition to the Coolpix 4500 User s Manual Nikon INSTRUMENTS INC Coolpix 4500 Camera Settings for Microscopy 1 Turn the camera on and select the infinity mode by pressing the Manual Focus MF button until the icon with two mountain peaks is displayed in the upper right of the monitor display Note that the flash turns off automatically when focus is set to infinity See p 57 Hold down the Mode button and simultaneously turn the command dial to select A Aperture Priority Auto mode The camera will set shutter soeed automatically for best results Rotate the command dial to set the F value to the lowest possible f stop number The approximate range value F2 5 F4 0 is dependent on the zoom setting Use the zoom buttons W T to fill the field of view Use the LCD screen to determine best image An image without black cut off corners or circular images is generally possible at mid range of the zoom setting Additional Settings in Menu Selection Press Menu for the Shooting Menu See p 97 for camera settings available onlyin the P S A and M mode and only when the camera is in shooting mode The following are recommended options for photomicrograp
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