Milwaukee Instruments Refrigerator Mi455 User's Manual
Contents
1. Ss ra TUTE EEA JL Lien This error message appears when the sample concentration exceeds 400 ppm The used standard solution is wrong This error message appears when the input readings mV exceed the input limits 0 1000mY TITRATION MESSAGES This screen appears each time a titration is running When the START STOP button is pressed the minititrator returns to the main screen The titration result expressed as concentration of sulphur dioxide in ppm mg L is displayed at the end of the titration process Press the START STOP button to return to the main screen This error message appears when the input reading exceeds the input limits 0 1000mY This screen appears when the sample concentration exceeds 400 ppm www milwaukeetesters com GENERAL TIPS FOR AN ACCURATE MEASUREMENT The instructions listed below should be carefully followed during testing to ensure best accuracy e Purge the peristaltic pump to have fresh titrant when starting a new analysis or calibration e Calibrate the peristaltic pump before performing an analysis e Analyze the wine immediately after the sample is obtained PUMP CALIBRATION PROCEDURE Warning The calibration of the pump must be performed each time the pump tube the reagent bottle or the pH electrode is changed It is recommended to perform the pump calibration before each set of me2. Milwaukee Wine Lab Mi inititrator Mi455 Free amp Total Sulphur Dioxide SAO www milwaukeetesters com Instruction Manual Mi455 FREE amp TOTAL SULPHUR DIOXIDE Minititrator for wine analysis TABLE OF CONTENTS EE SO MEASUREMENT PROCEDURE TOTAL SO MEASUREMENT PROCEDURE PUMP TUBE REPLACEMENT ELECTRODE CONDITIONING AND MAINTENANCE ACCESSORIES 20 www milwaukeetesters com FUNCTIONAL DESCRIPTION DISPLAY HOURGLASS ICON BATTERY STATUS ICON ERROR MESSAGE MEASURE STATUS CALIBRATION MODE INDICATOR MAIN DISPLAY MEASUREMENT UNIT TIMER MODE INDICATOR SECONDARY DISPLAY aA ARAZ dE FRONT PANEL TITRANT BOTTLE B LIQUID CRYSTAL DISPLAY LCD KEYPAD D ELECTRODE HOLDER REAR PANEL ORP ELECTRODE PERISTALTIC PUMP TUBE BEAKER PERISTALTIC PUMP BNC ELECTRODE CONNECTOR FUSE POWER CABLE CONNECTOR POWER SWITCH TAFT ZZ Or D Instruction Manual Mi455 FREE amp TOTAL SULPHUR DIOXIDE Minititrator for wine analysis KEYPAD 455 PUMP to start stop the pump B CAL to enter pump calibration mode C STIR to start stop the stirrer while in measurement or purging mode D START STOP to start stop titration or pump calibration Sulphur Dioxide Titrator GENERAL DESCRIPTION The
3. MEASUREMENT PROCEDURE Fill the 50 mL beaker up to 50 mL mark with the wine sample WINE SAMPLE Note It is important to be accurate in your measurement of wine sample Pipettes are recommended e Place the stir bar in the beaker and put the beaker in the minititrator top Fill the 20 mL beaker up to the 5 mL mark with the Mi555 003 Alkaline Reagent and add the content to the 50 mL beaker e Swirl the beaker and wait for 15 minutes e Using a clean pipette fill the 20 mL beaker up to the 5 mL mark with the Mi555 004 Acid Reagent and add the content to the 50 mL beaker Mi555 004 Acid reagent e Open refill cap on the ORP electrode and immerse the electrode approximately 2 cm 0 8 into the sample to be tested Do not touch stir bar with the tip of the electrode www milwaukeetesters com e Insert dosing tip into holder Ensure there is titrant in the dosing tip If not hold the E dosing tip over an empty container and press PUMP for a few seconds until the titrant is in the dosing tip The tip should be below the pump to ensure gravity flow and minimize the chance of air bubbles in the tube 55 006 E gt Stabilizer Add the content of one powder packet of Mi555 006 Stabilizer into the beaker e Press START STOP button to start titration The display will show during titration GO along with stirrer and pump tags blinking on Sy t
4. that it is largely governed by the total SO content of the wine The rate of binding decreases as the free SO concentration increases The exact relationship between free and bound total free SO will vary from wine to wine Below 30 60 ppm 33 to 50 of SO addition becomes bounded What remains is called free and it is divided in two parts The larger and relatively ineffective free part is www milwaukeetesters com bisulphite HSO The smaller part of the free is the active molecular SO The amount of molecular SO in your wine depends both on the level of free SO present as well as pH For instance at pH 3 2 the amount of free SO for 0 8 ppm molecular SO is 22 ppm At pH 3 5 you will need 43 ppm free essentially double Free SO concentration ppm for 0 8 ppm molecular SO Tet epee a ee EGOEREI EEI EE In most situations 0 8 ppm molecular SO during bulk storage and at bottling will provide you with adequate protection from oxidation and bacterial action This includes prevention of malolactic bacteria as well It is important to remember that the amount of free SO the wine depends on three things how much is added how much was present before the addition and how much of your addition promptly becomes bound The level at which molecular SO can be detected by the human senses is about 2 0 ppm This is also the level which is needed for maximum protection of your wine This is particularl
5. covered by the warranty Milwaukee Martini instruments reserves the right to make improvements in design construction and appearance of its products without advance notice THANK YOU FOR CHOOSING Milwaukee Sales and Technical Service Contacts Milwaukee s r l C so Leonardo da Vinci 48 50 21013 Gallarate VA ITALY Tel 39 0331 26 80 09 Fax 39 0331 26 80 33 e mail sales milwaukee 91 it Milwaukee Instruments Inc 2950 Business Park Drive Rocky Mount NC 27804 USA Tel 1 252 443 3630 Fax 1 252 443 1937 e mail milwaukee vol com www imilwaukeetesters com MANMI455 12 07
6. 9 Stir bar 5 pcs Mi0010 Titrator tube 2pcs Mi0020 Beaker 50 4 pcs Mi0021 Beaker 20 mL 4 pcs Recommendations for Users Before using this product make sure that it is entirely suitable for your specific application and for the environment in which it is used Operation of this instrument may cause unacceptable interferences to other electronic equipments this requiring the operator to take all necessary steps to correct interferences Any variation introduced by the user to the supplied equipment may degrade the instrument EMC performance To avoid damages or burns do not put the instrument in microwave ovens For yours and the instrument safety do not use or store the instrument in hazardous environments www milwaukeetesters com For your Safety don t use or store the instrument in hazardous environments To avoid damages or burns do not perform any measurement in microwave ovens WARRANTY This instrument is warranted against defects in materials and manufacturing for a period of 2 years from the date of purchase Electrodes are warranted for 6 months If during this period the repair or replacement of parts is required where the damage is not due to negligence or erroneous operation by the user please return the intrument electrode and probe to either distributor or our office and the repair will be effected free of charge Damage due to accidents misuse tampering or lack of prescribed maintenance is not
7. Mi455 is a low cost easy to use microprocessor based automatic titrator It has a simple and reliable peristaltic pump that ensure high dosing repeatability By performing pump calibration with the provided Milwaukee standards the instrument accuracy is assured The instrument comes with a pre programmed analysis method designed for Free and Total Sulphur Dioxide measurements on wine samples The instrument has a powerful and effective built in algorithm to analyze the shape of the electrode response and to determine the reaction completion This algorithm automatizes the analysis makes all the necessary calculations and assures a simple and effective interface for the user By simply pressing the START STOP button the instrument will automatically make the titration up to the equivalence point The result is immediately displayed in convenient units then the instrument is ready for another titration SIGNIFICANCE OF USE An important reason for adding SO is to avoid oxidation When there is oxygen around SO itself becomes oxidized before phenol compounds in the wine and so acts as an oxygen scavenger Also SO suppresses the activity of enzymes that cause browning and other problems What is really protecting your wine is molecular SO When you add SO depending of circumstances some of it immediately becomes bound The relationship between the amount of added SO and the amount of SO remaining free is complex It is clear however
8. PRESENT This is normal with elec trodes They will disappear when rinsed with water Reference Wire During transport tiny bubbles of air may form inside the glass bulb affecting proper EE functioning of the electrode These bubbles wre O RING can be removed by shaking down the elec trode as you would do with a glass 2 Junction thermometer 22 GA If the bulb and or junction is dry soak the 39 d i lution for at electrode in MA9015 Storage Solution for a d Giass least one hour ffs Glass Body If the filling solution electrolyte is more ORP Electrode www milwaukeetesters com than 2 cm 1 below the fill hole add MA9011 3 5M KCI Electrolyte Solution For faster response unscrew the fill hole screw during measurements STORAGE PROCEDURE To minimize clogging and assure a quick response time the glass bulb and the junction of he electrode should be kept moist and not allowed to dry out Replace the solution in the protective cap with a few drops of MA9015 Storage Solution or in its absence Filling Solution MA9011 Follow the Preparation Procedure before taking measurements Note NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER PERIODIC MAINTENANCE nspect the electrode and the cable The cable used for connecti
9. asurements e Using a clean pipette fill the 50 mL beaker up to the 50 mL mark with Mi555 001 Standard Mi555 001 STANDARD Note Failure to use clean pipettes will result in erroneus readings e Place the stir bar in the beaker and put the beaker in the minititrator top Fill the 20 mL beaker up to the 5 mL mark with the Mi555 005 Acid Reagent and add the content to the 50 mL beaker Mi555 005 Acid reagent Place the beaker into the beaker holder Place the probe holder on the top of the beaker and secure by turning clockwise Instruction Manual Mi455 FREE amp TOTAL SULPHUR DIOXIDE Minititrator for wine analysis e Open refill cap on the ORP electrode and immerse the electrode approximately 2 cm 0 8 into the sample to be tested Do not touch stir bar with the tip of the electrode e Ensure there is titrant in the dosing tip If not hold the dosing tip over an empty container and press PUMP for a few seconds until the titrant is in the dosing tip Add the contents of one powder packet of Mi555 006 Stabilizer into the beaker e Press CAL button The meter enters in pump calibration mode e Press START STOP in order to start the system calibration ZE At the end of the calibration DONE appears for a few seconds and the meter returns to d on E the measurement mode MOI milwaukeetesters com FREE SO MEASUREMENT PROCEDURE Using a clean p
10. he LCD nr At the end of the titration the Sulphur Dioxide concentration is displayed mg L ppm PUMP TUBE REPLACEMENT To remove the tube of the peristaltic pump follow next steps Fixing ri Detach the old tube system from the reagent bottle pened Grasp one fixing ring of the peristaltic pump tube Fixing rings e Pull the tube until it s taken out from its location Remove the other side of the tube To mount the new peristaltic pump tube follow next steps e Position one peristaltic pump fixing ring on its location Stretch the tube over the peristaltic pump cylinders Fix the second pump fixing ring on its location Attach the tube to the reagent bottle Note Purge the peristaltic pump until drops of reagent appears on the dosing tip by pressing the PUMP button Instruction Manual Mi455 FREE amp TOTAL SULPHUR DIOXIDE Minititrator for wine analysis FUSE REPLACEMENT To change the fuse follow next steps e Disconnect the power cord from the rear panel of the instrument Pull out the fuse holder located near the power cord connector Replace the fuse with a similar one e Push the fuse holder with the fuse in the appropriate place ELECTRODE CONDITIONING amp MAITENANCE PREPARATION PROCEDURE Remove the protective cap of the ORP elec trode MA924B 1 DO NOT BE ALARMED IF SALT DEPOSITS ZE ARE
11. ibration procedure is in fact the analysis of a known solution By doing this the instrument makes a differential analysis between the standard and the wine sample The pump volumetric debit and the real concentration of the titrant is compensated Only the sample volume has to be precisely known www milwaukeetesters com SPECIFICATIONS Range O to 400 ppm SO Resolution 1 ppm Accuracy 5 of reading Method Ripper titrimetric method Principle Equivalence point redox titration Sample volume 50 mL Electrode MA924B 1 included Pump debit 0 5 mL min Stirring speed 1500 Environment O to 50 C 32 to 122 F max 95 RH non condensing Power supply 220V 50Hz 1OVA Dimensions 208 x 214 x 163 mm 8 2 x 8 4 x 6 4 with beaker Weight 2200 g 77 6 oz Required Reagents Code Description Quantity test Mi555 001 Standard for Free amp Total SO 50 mL Mi555 002 Titrant for Free amp Total SO Mi555 003 Alkaline Reagent for Total SO 5 mL Mi555 004 Acid Reagent for Total SO 5 mL Mi555 005 Acid Reagent for Free SO 5 mL Mi555 006 Stabilizer for Free amp Total SO 1 packet This instrument is in compliance with CE Directives Instruction Manual Mi455 FREE amp TOTAL SULPHUR DIOXIDE Minititrator for wine analysis STARTUP Place the instrument on a flat table Do not place the instrument on direct sun light Connect the titrator mains socket with ground connection and
12. ipette fill the 50 mL beaker up to 50 mL mark with the wine sample Note It is important to be accurate in your measurements of Wine sample Pipettes are recommended Place the stir bar in the beaker and put the beaker in the minititrator top Fill the 20 mL beaker up to the 5 mL mark Acid reagent with the Mi555 005 Acid Reagent and add the content to the 50 mL beaker Place the probe holder on the top of the beaker and secure by turning clockwise Open refill cap on the ORP electrode and immerse the electrode approximately 2 cm 0 8 into the sample to be tested Do not touch stir bar with the tip of the electrode Insert dosing tip into holder Ensure there is titrant in the dosing tip If not hold the dosing tip over an empty container and press PUMP for a few seconds until the titrant is in the dosing tip The tip should be below the pump to ensure gravity flow Note Be aware of any air bubbles in the tube it could effect the results Add the contents of one powder packet of Mi555 006 Stabilizer into the beaker Instruction Manual Mi455 FREE amp TOTAL SULPHUR DIOXIDE Minititrator for wine analysis Press START STOP in order to start titration Ge The display will show during titration along with stirrer and pump tags blinking on the LCD l b Albe end of the titration the Sulphur Dioxide concentration is displayed in mg L ppm dane TOTAL SO
13. on to the instrument must be intact and there must be no points of broken insulation on the cable or cracks on the electrode stem or bulb Connectors must be perfectly clean and dry If any scratches or cracks are present replace the electrode Rinse off any salt deposits with water Probe Maintenance Refill the reference chamber with fresh electrolyte MA9011 Allow the electrode to stand upright for 1 hour Follow the Storage Procedure above CLEANING PROCEDURE e Wine deposits Soak in Milwaukee MA9016 cleaning solution for 1 hour Wine stains Soak in Milwaukee MA9016 cleaning solution for 1 hour IMPORTANT After performing any of the cleaning procedures rinse the electrode thor oughly with distilled water refill the reference chamber with fresh electrolyte and soak the electrode in MA9015 Storage Solution for at least 1 hour before taking measurements Instruction Manual Mi455 FREE amp TOTAL SULPHUR DIOXIDE Minititrator for wine analysis ACCESSORIES REAGENT SETS MA901 1 Electrode filling solution 230 mL MA9015 Electrode storage solution 220 mL MA9016 Cleaning solution 220 mL Mi555 001 Calibration standard 500 ml Mi555 002 Titrant solution 100 mL Mi555 003 Alkaline reagent 100 ml Mi555 004 Acid reagent for Total SO determination 100 mL Mi555 005 Acid reagent for Free SO determination 100 mL Mi555 006 Stabilizer reagent 40 pcs OTHER ACCESSORIES MA924B 1 ORP probe with 1 m cable 000
14. the correct voltage and frequency See the label on the instrument rear for this e Place the peristaltic pump tube on the pump See the Pump Tube Replacement section for the procedure e Remove the reagent bottle cap and place the bottle cap of the tubes set Place the reagent bottle in the appropriate place on the titrator top Connect the tubes with the peristaltic pump inlet tube is connected with the reagent bottle outlet tube is connected with the dosing tip Turn the instrument ON using the power switch from the rear panel of the instrument and wait until it displays dashes www milwaukeetesters com GUIDE DISPLAY CODES WRONG 10888 This screen appears for a few seconds each time the instrument is turned ON Main screen display Main screen display with stirrer active Purging mode message PUMP CALIBRATION MESSAGES This screen appears each time the meter enters pump calibration mode The meter is ready to start pump calibration by pressing the START STOP button This screen appears while pump calibration is in progress Pressing CAL or START STOP button the minititrator returns to the main screen This screen appears for a few seconds before returning to the main screen when pump calibration is done Instruction Manual Mi455 FREE amp TOTAL SULPHUR DIOXIDE Minititrator for wine analysis
15. y true in the case of sweet and most notably botrytised wines The Milwaukee Mi455 offers the possibility to test free or total SO in all the wines including the red ones that are difficult to test with manual methods because the color changes are hardly seen This meter is supplied with Reagents set for 20 titrations e Two 50 mL beakers e Two 20 mL beakers e Tubes set with cap ORP probe Stir bar Power cable One 230 mL bottle of Refill Solution e One 1 mL syringe e Instruction manual e Warranty code Note Save all packing material until you are sure that the instrument works correctly Any defective item must be returned in its original packing Instruction Manual Mi455 FREE amp TOTAL SULPHUR DIOXIDE Minititrator for wine analysis PRINCIPLE OF OPERATION Determination of sulphur dioxide in wine samples is made by titration of the sulphur dioxide present in wine with iodate In this procedure an excess of iodine is added to the wine sample and it is titrated with iodate For precise results it is very important to know the exact sample volume titrant volume and concentration The peristaltic pump has a good repeatability but the dosing volume depends on many factors as the diameter of the tube or the tube stretching To compensate for all this errors the pump need to be calibrated The calibration of the pump is also needed in order to have high precision of the titrations The cal
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