Leica dm750 User's Manual
Contents
1. Leica DM750 P i Manual MICROSYSTEMS Chapter overview Safety regulations 4 The Leica DM750 P 15 Get Ready 18 Care of the Microscope 38 N Dimensions Leica DM750 P Manual 2 Contents Safety regulations l CE Safety concept 5 Basic setup 25 Symbols used in this operating manual 6 Using the Aperture Condenser 26 Important Notes 7 Setting up Polarized Light 27 Instructions on use 8 Focusing 29 Health risks and dangers of use 10 Viewing Tube Adjustment 30 Information for the person responsible Koehler Set up 31 for the instrument 11 Objective Centration 32 Care instructions 12 Bertrand Lens Operation 34 Accessories maintenance and repair 13 Time Delay Shutoff 35 Electrical data and ambient conditions 14 TIE The Leica DM750 P 15 Ready Set Go 37 Introduction 16 Unpacking 17 Care of the Microscope 38 General maintenance 39 Get Ready 18 Mechanical Maintenance 41 Attaching Analyzer Module or Analyzer Bertrand Lens Module 19 Dimensions 42 Viewing Tube 20 Installing Objectives 21 Substage Condenser 22 Leica DM750 P ELITE Safety regulations Safety concept The individual modules of the Leica DM micros copy series include an interactive CD ROM with all relevant user manuals in several languages Keep it in a safe place and readily accessible to the user User manuals and updates are also available for2. Manual 4 Turn on the microscope using the switch on the bottom right hand side of the micro scope stand 25 Using the Aperture Condenser The condenser is furnished with an iris diaphragm which can be adjusted to match the effective numerical aperture of the objective 1 To open and close this diaphragm simply rotate the knurled condenser ring to the right or to the left so that the line on the rotating ring aligns with the objective magnification in use 2 To start fully open the iris diaphragm of the aperture condenser by rotating the ring to the extreme right Leica DM750 P Manual 26 Setting up Polarized Light H The polarizer is located under the 1 Loosen the polarizer locking thumbscrew 2 Rotate the polarizer to the zero position substage condenser in a swing in out which is located on the right hand side of mount Swing it into place and be sure it clicks the polarizer into place Leica DM750 P Manual 27 Setting up Polarized Light cont The Analyzer is located in the module 3 If you have an Analyzer Bertrand Lens 4 Increase the illumination intensity slightly between the viewing tube and the stand module swing in the Analyzer by flipping You will notice while looking into the either in a slider module or in an Analyzer the lower rocker of the module to the microscope with the Polarizer and Analyzer Bertrand Lens module If you have a slider slide right the A position
3. rately Also there is a magnetic storage location for the objective centering tools in the back of the Analyzer or Analyzer Bertrand Lens modules Leica DM750 P Manual 33 Bertrand Lens Operation If you have an Analyzer Bertrand Lens module you can view a conoscopic image through the eyepieces 1 Bring the portion of the specimen you would like to view in conoscopy with the Bertrand Lens to the center of the field 2 Swing in the Bertrand Lens by flipping the upper rocker of the Analyzer Bertrand Lens module to the B position Be sure the Polarizer and Analyzer are also in position Leica DM750 P You can now view the conoscopic image through the eyepieces 3 If necessary you can fine tune the Bertrand Lens centration with the tools provided Manual 4 If you have an Analyzer Bertrand Lens with a focusable Bertrand Lens you can now turn the knob on the upper rocker to focus the Bertrand Lens image 34 Time Delay Shutoff The Leica DM 750 P is equipped with Li a time delay shutoff capability which automatically turns the illumination off after 2 hours of no changes in the intensity control e The time delay shutoff IS enabled as a default Leica DM750 P Changing the status of the time delay shutoff 1 Rotate the intensity control to the lowest setting Turn the power on Rotate the intensity control to full high intensity then back to full low intensity all within 5 se
4. Open the field diaphragm until the iris leaves disappear just beyond bring the leaves of the field diaphragm into sharp focus the field of view Leica DM750 P Manual 31 Objective Centration Tools used 2 Focus the polarizing sample e Centering keys 3 Move a noticeable point of the sample to the center of the crosslines 1 Retrieve the two centering keys from the packaging and insert the centering tools into the centering holes located above the objective in use Engage the ball end of the centering tools into the set screws which are hidden in the centering holes 4 Loosen the stage brake and rotate the stage until the noticeable point of the sample is furthest away from the center of the cross lines This may even be outside of the field of view This indicates the objective is not centered to the stage Leica DM750 P Manual 32 Objective Centration cont 5 Adjust the objective centering screws 6 Move the specimen not the objective 7 Check to make sure that the noticeable until the noticeable point of the sample is centering screws so that the noticeable point stays at the center of the crosslines midway between the furthest position and point is at the center of the crosslines once when the stage is rotated If not repeat the the center of the crosslines This moves the again centering process objective center closer to the stage center Each objective must be centered sepa Li
5. Acetone xylene or nitro containing thin manufacturer ners must NOT be used It is advisable to enter a service agreement e Never use chemicals to clean colored with Leica Service Leica DM750 P surfaces or accessories with rubberized parts This could damage the surfaces and specimens could be contaminated by abraded particles Manual Accessories maintenance and repair Accessories Only the following accessories may be used with the Leica DM750 P microscope e The Leica accessories described in this user manual Maintenance e The Leica DM750 P microscope is basically maintenance free To ensure that it always operates safely and reliably we recommend that you take the precaution of contacting the responsible service organization Repairs and service work e Only original Leica Microsystems spare parts may be used Before opening the instruments switch off the power and unplug the power cable Avoid contact with powered electrical e Other accessories provided that these have H You can arrange for periodic inspections circuits which can lead to injury been expressly approved by Leica as being or if appropriate conclude a mainte technically safe in this context nance contract with them Service address In case of problems please contact us as follows e Itis advisable to enter a service agreement with Leica Service e For maintenance and repair only OEM spare parts may be used Leica Mi
6. cal devices outdoors Install electrical devices at least 10 cm from the wall and away from flam mable substances Avoid large temperature fluctuations direct sunlight and vibrations These conditions can distort measurements and micrographic images In warm and warm damp climatic zones the individual components require special care in order to prevent the build up of fungus Manual Non intended use Using the instrument in any way A contrary to the specifications in the user manual can lead to bodily harm and damage to objects Doing so can impair the functions of the protective measures provided Never install any other plug NEMA 5 15P or unscrew any mechanical components unless expressly instructed to do so in the instructions H The devices and accessories described in this operating manual have been tested for safety and potential hazards Instructions on use continued The responsible Leica affiliate must be A consulted whenever the instrument is altered modified or used in conjunction with non Leica components that are outside of the scope of this manual Unauthorized alterations to the device A or noncompliant use shall void all rights to any warranty claims Leica DM750 P Transport If at all possible use the original packaging for shipping or transporting individual modules In order to prevent damage from vibrations disassemble all moving parts that according to the user manual can be a
7. cause the following e Hazards to personnel e Functional disturbances or damaged instruments Leica DM750 P Danger due to hot surface This symbol warns against touching A accessible hot surfaces e g those of light bulbs Important information This symbol indicates additional infor mation or explanations that intend to provide clarity Explanatory notes This symbol within the text points to addi tional information and explanations Manual Important Notes Description The Leica DM750 P microscope meets today s state of the art of technology Nevertheless hazards may still arise during operation The potential risks are described below A Before installing operating or using the instruments it is required to read this user manual In particular please observe all safety instructions Contact address Leica Microsystems Switzerland Ltd Industry Division Max Schmidheiny Strasse 201 CH 9435 Heerbrugg Switzerland Leica DM750 P User Manual This user manual includes important instruc tions related to operating safety maintenance and accessories Your Leica DM750 P microscope comes with an interactive CD ROM with all relevant user manuals Keep it in a safe place and readily accessible to the user User manu als and updates are also available for you to download and print from our web site at www leica microsystems com Manual Accessories from third party suppliers You can co
8. parts may be used in servicing the product After service work or technical modifica tions the unit must be reconfigured with observance to our technical requirements If the unit is modified or serviced by unau thorized persons is improperly maintained as long as maintenance was not carried out by us or is handled improperly Leica will not accept any liability The electric installation in the building must conform to the national standard e g current operated ground leakage protec tion fault current protection is suggested Care instructions General instructions Cleaning coated parts and plastic parts e Protect the Leica DM750 P microscope o Cleaning glass surfaces Dust and dirt particles should be removed against damp vapors acids alkalis and corrosive substances Do not store chemi with a soft brush or lint free cotton cloth Remove dust using a fine dry and grease free brush made from hair by blowing with a squeeze blower or by using a vacuum cals in the vicinity e Remove coarse debris with a moistened disposable cloth e Remove dirt using a clean cloth that has Protect the Leica DM750 P microscope from been moistened with distilled water oil and grease Never grease or oil mechani e Clinging dirt can be removed using petro cal parts or sliding surfaces leum ether or alcohol e Remove tough dirt using alcohol chloro form or petroleum ether Follow the instructions of the disinfectant e
9. you to download and print from our web site at www leica microsystems com This operating manual describes the special functions of the individual modules of the Leica DM microscopy series and contains important instructions for their operational safety main tenance and accessories The Safety concept booklet contains addi tional safety information regarding the service work requirements and the handling of micro scope accessories and electrical accessories as well as general safety instructions Leica DM750 P You can combine individual system articles with articles from external suppliers Please read the user manual and the safety requirements of the supplier Before installing operating or using the instru ments read the user manuals listed above In particular please observe all safety instruc tions To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in these user manuals Manual Symbols used in this operating manual Warning of a danger This symbol indicates especially impor A tant information that must be read and complied with Failure to comply can cause the following e Hazards to personnel e Functional disturbances or damaged instru ments Warning of hazardous electrical voltage This symbol indicates especially impor tant information that is mandatory to read and observe Failure to comply can
10. 13 11 74 Manual Leica DM750 P 13 78 Manual Leica DM750 P
11. Analyzer in while in place and no specimen that the field will the Analyzer into position ensuring the upper rocker of the Analyzer look dark If not rotate the polarizer slightly Bertrand Lens Module is flipped to the left until you have the darkest field of view the O position Bertrand Lens out Leica DM750 P Manual 28 Focusing Focusing 3 Raise the microscope stage using the 4 Look into the eyepieces and adjust the illu 1 Place a polarized light specimen on the coarse adjustment knob until you reach its mination intensity so that it is comfortable Stage positive stop or the specimen touches the for viewing objective 2 Rotate the 10x objective into working posi 5 Using the fine focus adjustment knob bring tion the specimen into sharp focus Leica DM750 P Manual 29 Viewing Tube Adjustment Adjust the eyetubes for your interpupillary distance 1 Fold or unfold the eyetubes until you see one illuminated circle The viewing tubes maintain a constant tube length for all interpupillary settings This means that a change of interpupillary distance does not affect parfocality magnifica tion or calibrations that depend on magnifica tion A To correctly set the eyepieces be sure the crosshair eyepiece is seated securely in the slot of the right eyetube Leica DM750 P 2 Focus the crosshair reticle by gripping the knurl and rotating the top of the eyepiece until the reticle is in clear focu
12. P Manual 22 Substage Condenser cont 4 Completely loosen the two thumbscrews 6 Raise the condenser to its highest position 7 Tighten the thumbscrews to roughly center on the condenser mount using the condenser focus on the left hand the substage condenser so that the top lens side of the substage of the condenser is centered beneath the objective in use You will more accurately center and focus the condenser when you reach section Koehler Setup 5 Slide the substage condenser into the fork mount located underneath the stage by aligning the positioning pin on the condenser into the slot on the back of the fork Leica DM750 P Manual 23 Get Set Basic setup As a Safety precaution the power cord has been grounded to the microscope base Never use an adapter between the power cord and the power source it will render the grounding feature ineffective Work Surface A Always use your microscope on a hard stable surface Power 1 If the power cord is not already attached attach it securely to the back of the micro scope Leica DM750 P Plug the microscope power cord into a suitable grounded electrical outlet A grounded 3 wire cord is provided Set the illumination control knob is located on the bottom left hand side of the micro scope stand to the lowest setting to start The illumination control knob allows you to adjust the intensity of light produced by the illumination system
13. The microscope should always be covered with the plastic dust cover provided with the instrument when it is not in use If any optical surface becomes coated with dust or dirt clean the surface by blowing it off with a syringe or brushing it off with a camel hair brush before attempting to wipe the surface clean Optical surfaces should be cleaned with a lint free cloth lens tissue or a cotton swab moistened with methanol or commercially available glass cleaner Do not use alcohol Leica DM750 P It is very important to avoid the excessive use of solvent so use them sparingly The lint free cloth lens tissue or cotton swab should be moistened with solvent but not be wet enough for the solvent to seep around the lens No part of the microscope is quite so vulner able to collecting dirt dust and oil as the front lens of the objective Whenever you encounter lack of contrast cloudiness or poor definition carefully check the condi tion of the front lens with a magnifier The lower magnification objectives have fairly large front lenses and can be cleaned with a cloth or lens tissue wrapped around a finger and moistened with methanol Do not use alcohol Cleaning 40x and 100x objectives requires more care Note To achieve the high degree of flatness obtained with the higher magni fication objectives the objective has a small Manual concave front lens of fairly short radius or curvature The surface of this front
14. conds Manual e The LED Illumination will flash to indicate the time delay shutoff status was changed e The LED Illumination will flash two times slowly then stay on when you disable the Time Delay Shutoff e The LED Illumination will flash three times quickly then stay on when you enable the Time Delay Shutoff When you turn the power off and then turn the power on again the system will be in the last Time Delay Shutoff status Enabled or Disabled and you will not see any blinking Leica DM750 P Manual 36 Ready Set Go Now all you need to do is change objectives set the aperture condenser and field diaphragm appropriately for the objective magnification in use and enjoy the view Leica DM750 P Manual Care of the Microscope General maintenance General e The cord wrap allows you to wrap the cord e Proper storage of accessories in the appro Always carry the microscope using two in such a way that only the length you need priate positions is important to keep them hands There is a handle on the back of is extended safe from loss or damage There are storage places for the objective centering tools and for two compensators in the rear of the Analyzer Bertrand Lens module the microscope and an undercut in the front for this purpose Leica DM750 P Manual 39 General maintenance cont Keeping all optical components clean is important for maintaining good optical performance
15. crosystems Switzerland Ltd Industry Division Max Schmidheiny Strasse 201 CH 9435 Heerbrugg Switzerland Phone 41 71 726 33 28 Leica DM750 P Manual 13 Electrical data and ambient conditions Fuse Replacement Unplug the instrument before changing any fuses The Leica DM750 P has two fuses which are located behind the power cord receptacle A Use only fuse type 5x20 mm 1 Amp 250V Quick acting 13RFAG30003 Electrical data Input 100 240 V 50 60 Hz 5 W 3 W LED Leica DM750 P Environment Temperature for use 10 C 40 C Storage temperature 20 C 52 C 50 F 104 F Manipulation shock 25 mm on 50 mm hard wood Transport shock unpacked 100g 6ms Transport shock packed 800 mm free fall Transport vibrations unpacked 5 200 Hz 1 5g Atmospheric pressure in use and for storage 500 1060 mBar Humidity in use and for storage 20 90 Installation Category Il Overvoltage Category Pollution degree 2 Manual 14 The Leica DM750 P Introduction Thank you for purchasing the Leica DM750 P Polarizing Microscope System from Leica Microsystems The Leica DM750 P is a highly adaptable instrument with superior optics and precision engineering for educational and basic industrial polarized light microscopy applica tions Leica DM750 P Manual Unpacking e Carefully remove the microscope and any components from the packing carton e Check the com
16. e The Leica DM750 P microscope may not be operated unless it is in proper functioning condition Information for the person responsible for the instrument Ensure that the Leica DM750 P microscope is used only by qualified personnel Ensure that this user manual is always avail able at the Leica DM750 P microscope Carry out regular inspections to make certain that the authorized users are adher ing to safety requirements When instructing new users do so thor oughly and explain the meanings of the warning signs and messages Assign individual responsibilities for start ing operating and servicing the instru ment and monitor the observance of these responsibilities Leica DM750 P Information for the person responsible for the instrument Do not use the Leica DM750 P microscope unless it is in perfect condition Inform your Leica representative or Leica Microsystems Schweiz AG Industry Division 9435 Heerbrugg Switzerland immediately of any product defect that could potentially cause injury or harm If you use accessories made by third party manufacturers with the Leica DM750 P microscope be sure that each such manu facturer confirms the safety engineering harmless usability of the product and observe the product s user manual Modifications and maintenance of the Leica DM750 P microscope may only be performed by professionals expressly authorized by Leica Manual Only original Leica replacement
17. lens can be readily cleaned with a toothpick covered with a cotton tip or with a small cotton swab Moisten the cotton with metha nol and squeeze it almost dry Wipe the front lens lightly without applying undue force or scrubbing action Make sure that the cotton tip contacts the concave lens surface Check the objective with a magni fier after cleaning If you need to remove the microscope s viewing body be careful not to accidentally touch the outer lens surface located on the underside of the body Fingerprints on this surface will reduce image clarity This lens can be cleaned in the same manner as objectives and eyepieces 40 Mechanical Maintenance Mechanical Maintenance e Use the dust cover It is the most impor tant way to keep the microscope in good mechanical and physical condition e The Leica DM750 P has a durable finish All non optical parts of the instrument can be cleaned with methanol naptha or soap and water Avoid the use of all other organic solvents when cleaning the instrument Leica DM750 P Manual 41 Dimensions i F it ma Y TO li 4 EI A ee lr JA mi Be so E ll _ r T r t l i LE i ha ha a I A Leica DM750 P Manual BS 327 1281 17 98 455 809 13 78 298 1
18. mbine individual system articles with articles from external suppliers Please read the user manual and the safety requirements of the supplier Original condition To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in these user manuals Legal requirements Adhere to general and local regulations relat ing to accident prevention and environmental protection EC Declaration of Conformity Electrically operated accessories are constructed based on the state of the art of technology and are provided with an EC Declaration of Conformity Instructions on use Intended use The Leica DM750 P microscope is an optical instrument which uses magnification and illu mination to improve the visibility of objects It is used for observing and documenting The Leica DM750 P microscope may be used only in closed rooms and must be placed on a solid floor The Leica DM750 P microscope can be used in clean rooms without any problems Position the Leica DM750 P microscope A so that it can be disconnected from the power supply at any time The power cord must remain accessible at all times Leica DM750 P Place of use Only use the instruments in closed dust free rooms and between 10 C and 40 C Protect the devices from oil chemicals and extreme humidity If using the devices outdoors protect them from dust and moisture Never use electri
19. pe of the module 3 Insert the dovetail of the polarizing view ing tube into the module and tighten the set screw firmly without forcing it This precisely positions the body onto the opti cal axis of the microscope 4 Insert the eyepieces into the eyetubes Leica DM750 P Manual 20 Installing Objectives If you have purchased a standard micro 1 As you rotate the nosepiece clockwise H scope configuration you will notice the attach the objectives by screwing them objectives are already installed on the nose into the nosepiece holes starting with the piece and the substage condenser is already lowest magnification and advancing to the installed on the stand In this case go to section highest magnification Operation If you purchased your Leica DM750 P by components and not by standard configu ration please continue in this section A Always rotate the nosepiece by using the knurled nosepiece ring Leica DM750 P Manual 21 Substage Condenser Substage Condenser 2 Adjust the condenser mount to its lowest 3 Swing the polarizer which is connected to HA The Leica DM750 P has an open substage position using the condenser focus knob the bottom of the condenser mount to the condenser mount Therefore the on the left hand side of the substage left condenser needs to be installed 1 Raise the stage to its highest position using the coarse focus knob on the right side of the microscope Leica DM750
20. ponents against the planned configuration e Optional items such as contrast accessories camera adapters cameras and carrying cases are not shipped as part of the stan dard equipment These items are delivered in separate packages e Please do not discard any of the packing materials They should be used for safely storing and transporting the instrument should the need arise Leica DM750 P Manual 17 Get Ready Attaching Analyzer Module or Analyzer Bertrand Lens Module 2 Align the shape of the module with the shape of the microscope stand Tools used o Allen key 1 Slightly unscrew the set screw located on top of the stand with the Allen key tool provided 3 Insert the dovetail of the module into the stand Analyzer Bertrand Lens Module shown Leica DM750 P Manual 4 Tighten the set screw to lock the module into place Viewing Tube 1 Slightly unscrew the set screw located on the top of the Analyzer module 5 Ifyou wear eyeglasses for microscope view ing keep the rubber eyeguards folded down If you do not wear eyeglasses you may find it useful to unfold the rubber eyeguards in order to block out ambient room light Install the eyepiece with the crossline H reticle into the right eyetube so that the raised feature on the eyepiece aligns and is seated with the orientation slot in the right eyetube 2 Align the shape of the polarizing viewing tube with the sha
21. s 3 While looking through only the crosshair eyepiece bring the specimen into focus with the fine focus of the microscope A It may help to cover your left eye 4 Now switch to look only through the other eyepiece focusing eyepiece without crosshair with the other eye Manual This time focus the specimen by using A the focusing capability in the focusing eyepiece Do not raise or lower the stage to focus 5 Grip the knurl on the focusing eyepiece with one hand and rotate the top of the eyepiece with the other hand until the specimen is in focus with this eye and this focusing eyepiece This corrects for any vision differences between your right eye and left eye 6 Now switch to a high magnification objec tive not an oil objective and focus the microscope while viewing with both eyes The higher magnifications have a shal lower depth of field Therefore after focusing with a high magnification you will find that when you change to a lower magni fication you only have to adjust the fine focus slightly if at all Koehler Set up Koehler Set up 1 Close the Koehler field diaphragm on the base of the microscope so 3 Turn the condenser centering thumbscrews simultaneously to center that its iris leaves are present within the field of view when looking the image of the field diaphragm in the field of view into the eyepieces 2 Use the condenser focus knob on the left hand side of the substage to 4
22. ssembled and disas sembled by the customer and pack them sepa rately Disposal Disposal must comply with locally applicable laws and regulations Manual Integration in third party products When installing Leica products into third party products the manufacturer of the complete system or its dealer is responsible for follow ing all applicable safety instructions laws and guidelines Health risks and dangers of use Health risks Workplaces with microscopes facilitate Li and improve the viewing task but they also impose high demands on the eyes and holding muscles of the user Depending on the duration of uninterrupted work asthenopia and musculoskeletal problems may occur For this reason appropriate measures for reduction of the workload must be taken e Optimum workplace layout e Frequent changes of activity e Thorough training of the personnel giving consideration to ergonomic and organiza tional aspects Leica DM750 P The ergonomic design and construction of the Leica microscopy series are intended to reduce the exertion of the user to a minimum Danger of infection Direct contact with eyepieces is a poten A tial transmission method for bacterial and viral infections of the eye A The risk can be kept to a minimum by using personal eyepieces for each indi vidual or detachable eyecups Manual Dangers during use e The Leica DM750 P microscope may only be connected to a grounded socket
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