Leica EZ4 User's Manual
Contents
1. Leica EZ4 without Eyepieces 107 EA aa tor J a 9 Leica E Series Leica EZ4 without Eyepieces Continued2. Leica ES2 Leica EZ4 Leica EZ4 HD Manual MICROSYSTEMS Congratulations You have just purchased a Leica E Series stereo microscope an excellent choice The Leica E series is perfectly suited for practical instruc tion in schools and universities as well as for routine tasks in the laboratory and industrial production and testing Viewing entire objects under magnification imparts perspectives and discoveries that would not be possible with the naked eye Leica E Series In developing our stereomicroscopes we have placed great emphasis on simple self explana tory operation However please take the time to read the instruction manual and the notes on operating safety to learn about all the features and capabilities of your instrument so that you can use it safely and to its best advantage Should you have any questions please consult your local Leica representative We are gladly at your service Manual afety and Use eica EZ4 and Leica EZ4 HD yepieces only for Leica EZ4 Photography Using the Leica EZ4 HD he Camera Menu A Un A gt gy n eb Mm lt Mm Mm oy RUS fy ORSI CR Mm ES Ed G E a are and Transport n fo dd pecifications cli N Dimensions Leica E Series ETT Table of Contents TO vepicces only for Leica EZ4 Overview Leica ES2 11 Changing Eyepieces Only for Leica EZ4 Open Version 30 Transport Placement and Stora
3. 1 1 25 1 6 2 2 5 3 3 5 Different total magnifications are possi ble depending on which eyepieces are used with the EZ4 The magnification of the eyepieces is indicated on their outer surfaces e g 10x 20 16x 16 or 20x 12 Manual Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focusing drive too easy or too difficult to adjust No problem the resistance can be adjusted individually depending on your perso nal preferences 1 Grip the drive knobs with both hands and turn them towards each other until the desired resistance is reached during focu sing Leica E Series Manual Eyepieces only for Leica EZ4 Changing Eyepieces Only for Leica EZ4 Open Version Changing eyepieces Various eyepieces are available for the Leica EZ4 allowing the stereomicroscope to be adap ted even better to the specific requirements The following eyepieces are available for selec tion e Eyepieces replaceable fixed or adjustable 10x 20 16x 16 or 20x 12 e Eyepieces for eyeglass wearers 10x 20B and 16x 15B fixed and adjustable The interchangeable eyepieces also offer the following advantages e The dioptric range is adjustable from 5 to 5 e Measurement graticules can be used see page 34 and the following Leica E Series Adjustable eyepieces for eyeglasses with straight eyecups Manual Eyepieces and Eyecups Only for Leica EZ4 Open Version Eyecups The eye
4. 10x eyepieces of the Leica EZ4 HD have a field of view of 20 mm Unlike the circular object field through the eyepieces the image section of the camera is rectangular and has a diagonal measurement of 16 4 mm There fore the image field of the camera is smaller than the visible object field through the eyepi eces Installing and Connecting 1 Placethe DVD provided into the computer s disk drive and follow the instructions for loading the software 2 Connect the camera and the computer using the USB cable Leica E Series 3 Follow the instructions in the software for adjusting and acquiring an image The LED changes color from yellow to Li red You can now start the software Once you have done so the LED flashes yellow briefly then stays steady green UAAP S Manual PC Mode Limitations Aspect ratio If the Leica EZ4 HD is connected to a 1 computer and is in PC mode images are always output in a 4 3 size ratio and in a lower resolution even if a 16 9 monitor or television set is connected via HDMI The image is neces sarily compressed on such a device In this case we recommend that you not connect the television to the microscope but to the computer as a second monitor Remote control If the Leica EZ4 HD is in PC mode the l optional remote control cannot be used The menus of the camera are also no longer displayed on the monitor Instead all settings are configured on the computer
5. Five diodes greatest Top three diodes shadow Two diodes side light Incident light off Switch on transmitted light brightness for incident less uniform lighting for emphasizes structures and for transparent objects light objects objects with highly uneven increases the contrast and transmitted light surfaces specimens Leica E Series ETE Focusing A Focusing raises and lowers the stereomi Focusing croscope using the focusing drive The 1 Set the magnification changer to the lowest specimen is brought into sharp focus as soon as level 8 it is in the focal point of the objective The focusing drive can be operated either left or right handed 2 Set the working distance to approx 100 mm for coarse focusing S Focusing drive torque Leica E Series Manual 3 Set the magnification changer to the second level 35 4 Use the focusing drive once again for fine focus After you have adjusted the image sharp 1 ness at high magnification it remains constant even if you switch magnification levels parfocal Magnification Display You can read the configured magnifica i tion on the right hand rotary knob of the Leica EZ4 models The additional magnification provided by the eyepieces is already incorpora ted in the scale Leica E Series Leica EZ4 models with open tubes On the EZ4 model without eyepieces 1 only the magnification factors of the magnification changer are indicated 0 8
6. HD is not connected to a computer The resolution is currently limited to 720x480 pixels and cannot be changed QUALITY of the image capture A higher image quality can slow down the image capture process Two quality levels are available for selection NORMAL and HIGH SHARPNESS internal camera sharpness Define the amount of sharpening to be applied to the image Manual SETUP CAMERA Camera Settings COLOR SET DATE TIME PRESS OK RESET CAMERA PRESS OK RESOLUTION FLIP IMAGE 0 gt SETUP CAMERA OVERLAY NONE SETUP USER CAM MODE PC MODE Main menu Submenu A The camera settings enable you to control internal parameters such as the date or time or to reset the camera You can also switch the camera from HD mode to PC mode or vice versa SET DATE TIME This command sets the date and time as well as the format for display ing them The following formats are available for selection YYMMDD DDMMYY MMDDYY Leica E Series RESET CAMERA This command resets the camera to the factory settings All user informa tion such as white balance resolution etc is reset FLIP IMAGE Rotates the live image by 180 Overlay Shows the following on the HD monitor superimposed over the live image Leica logo histogram date time and image counter CAMERA MODE This allows you to toggle the camera between PC and HD mode You can also toggle between PC and HD mode by pressing the black button fo
7. color temperature This is particularly necessary if you are working with an external light source that can be switched from yellow low inten sity to blue high intensity The new white balance generates a neutral background for both types of illuminator Restoring Factory Settings Factory settings 1 Press the red multifunction button for 10 seconds to delete all individual settings and reset the instrument to the factory settings ee sec Leica E Series Manual The Camera Menu If you purchased the optional remote you will have access to the camera menu Leica E Series Manual Optional Remote Control The remote control does not work if the A camera is in PC mode Leica E Series ETE RWN gt o NA DM Start video recording Stop video recording Save still image to SD card Stop continue live image Pause play movie clip Playback mode Show hide information box Arrow keys for navigation OK confirm Call up the camera menu Viewing Images and Movies with the Optional Remote Control If you have purchased the optional 3 To return to live view press any button on i remote control you can display the the camera or press the key 1 2 or 9 on the 007 0109 ICC5000 P4 images and videos captured by the HD camera remote control see previous page directly on a HD screen 1 Miniature view press 1x e Select miniature gt A and v e Show full size image or play back vid
8. OLUTION 58 SETUP CAMERA Camera Settings 59 SETUP USER User defined Settings 60 Predefined Illumination Scenarios 61 are and Transport Care and Transport 63 pecifications Camera Module 65 Leica ES2 68 Leica EZ4 HD 70 Leica EZ4 10x Eyepieces 72 Leica EZ4 without Eyepieces 74 Leica E Series ETT Safety and Use Safety Notes A Before installing operating or using the instrument it is mandatory to read this user manual In particular please observe all safety instructions The Safety Concept brochure contains additi onal safety information regarding service work requirements and the handling of the micro scope accessories as well as general safety inst ructions You can combine individual system articles with articles from external suppliers Please read the user manual and the safety requirements of the supplier To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in these user manuals Leica E Series Warning of a danger This symbol indicates especially impor A tant information that is mandatory to read and observe Failure to follow these inst ructions may pose hazards to personnel cause instrument malfunctions or damage the instru ment Hazardous electrical voltage This symbol indicates especially impor tant information that is mandatory to read and observe Failure to follo
9. OSURE GAIN and GAMMA until you obtain the desired results Automatic exposure EXP MODE ATG 1 Press the amp button on the remote control GAIN gt 2 Call up the command for automatic exposure see diagram at the left ERE di 3 Set the value for EXP MODE to AUTO Mainmena submenu 4 Correct the values for BRIGHTNESS and GAMMA until you obtain the desired results Leica E Series Manual RESOLUTION COLOR LIVE 1280X1080 CAPTURED 2048X1536 gt RESOLUTION MOVIE 720X480 SETUP CAMERA QUALITY HIGH SETUP USER SHARPNESS HIGH MAIN MENU SUBMENU The RESOLUTION menu enables you to define the resolution for the live image on the computer still image capture and video capture individually This provides optimum results in every situation LIVE resolution of the live image Defines the resolution of the live image The following resolutions are available e For direct connection of the Leica EZ4 HD to a monitor or TV set 1280x720 or 19201080 16 9 aspect ratio e For connection to a computer 640x480 1024x768 and 1440x1080 4 3 aspect ratio Leica E Series CAPTURED resolution of the captured image Defines the resolution of the captured image if the Leica EZ4 HD is not connected to a computer The following resolutions are available 640x480 1024x768 16001200 and 2048x1536 MOVIE resolution of videos Defines the resolution of the recorded videos if the Leica EZ4
10. Series Manual General Instructions General Instructions e The software for control is included on the DVD provided with the instrument e Install the software before connecting the Leica EZ4 HD to the PC or Mac e As soon as the EZ4 HD is connected to a power cable and the main switch is turned on the integrated camera is also ready for use e Ifa USB cable is not connected and there is no SD card in the card slot of the Leica EZ4 HD the status LED of the instrument lights up RED Leica E Series Manual Introduction The Leica EZ4 HD puts some extremely interes ting additional functions at your fingertips The integrated digital camera is designed for the sole purpose of giving you the best possible quality for your documentation with particu larly easy handling Real Full HD display The integrated HDMI output allows the micro scope image to be output to a High Definition HD capable display unit monitor television set or projector where the following resolu tions are possible e 1920x1080i Full HD This resolution provides the best possible imaging perfor mance on a Full HD capable display unit e 1280x720p HD ready This resolution is suitable for display on televisions labeled HD ready Leica E Series Leica Application Suite EZ Together with the Leica EZ4 HD you have also obtained the Leica Application Suite EZ soft ware standard LAS on request It allows vari ous tasks
11. Series Manual Graticules Preparation Care instructions The graticule must always be clean and dust free Never touch the graticule with bare hands Always hold the graticule by the edges Leica E Series Variant 1 10x and 20x eyepieces The 10x and 20x adjustable eyepieces include an insert at the bottom with a snap mount 1 Gently press the two small tabs on the snap mount together and pull out the insert ETE Variant 2 16x eyepieces On the 16x adjustable eyepieces the insert is fixed to the eyepiece by a screwed connection 1 Screwthe insert out counterclockwise Inserting the Graticules Inserting the graticules is the same for all eyepi 4 Put the insert back into the eyepiece 6 Align the graticules by turning the eyepiece ece types Depending on the variant you have to in the tube either press or twist the insert into place 1 Place the insert with the knurled side on the work surface 2 Grasp the graticule so that you see the scale in laterally correct form 3 Carefully take hold of the edge of the grati cule and push it sideways into the holder in the insert Make sure that both plastic holders snap into place 5 Leica E Series Manual Photography Using the Leica EZ4 HD Leica EZ4 HD Overview of the instrument 1 SDcardslot 1 USB connection 2 Status LED 2 Pinhole switch 3 Black multifunction button 3 HDMI connection 4 Redmultifunction button Leica E
12. annot be captured directly to an SD card All images are stored on the PC If the camera is in HD mode it is not detected by the Leica software All images are stored on the SD card The microscope remains in the last selec l ted mode HD or PC mode until you change it You can do this automatically by star ting the Leica software or manually by pressing and holding the black multifunction button for 5 seconds Manual Optional remote control You can use the optional remote control 1 only if the camera is HD mode with the exception of the i button which you can use to show various information on the monitor even in PC mode Capturing Images without a Computer in HD Mode Saving to SD card Images can be saved directly to an inser Li ted SD card The card has to be formatted using the FAT file system i When the SD memory card is full the LED on the camera is illuminated in red No more images can be captured Leica E Series Changing between PC and HD mode In order to take photos without a compu 1 ter you must be in HD mode not PC mode After the camera is switched on it is in the last selected mode However it switches to PC mode whenever the Leica software is started 1 To switch between PC and HD mode press and hold the black multifunction button for 5 seconds Manual Detecting the active mode e The camera is in HD mode when the LED status display is illuminated in red e Th
13. atic Shutoff Automatic shutoff The factory settings of the EZ4 models 1 switch the instrument off automatically after one hour without user interaction This automatic shutoff helps to conserve the light emitting diodes and save energy Leica E Series Disabling the automatic shutoff To disable the automatic shutoff follow these steps 1 Switch the microscope off 2 Press and hold the and buttons on the light control unit simultaneously while you switch on the microscope The LEDs flash twice slowly and then remain switched on The automatic shutoff is now disa bled Manual Enabling the automatic shutoff To re enable the automatic shutoff follow these steps 1 Switch the microscope off 2 Press and hold the and buttons on the light control unit simultaneously while you switch on the microscope The LEDs flash three times slowly and then remain switched on The automatic shutoff is now re enabled Illumination types Three level incident light illumination Regulating brightness The incident light illumination is made The brightness is adjusted using the and i up of 5 LEDs Two three or five diodes are keys and affects all switched on LEDs switched on depending on the level Accord ingly the angle of incidence of the light beam on the object is switched from high to low DD O Press 1x Press 2x Press 3x Press 4x Press 1x
14. cups can be replaced Risk of infection Direct contact with eyepieces is a poten A tial transmission method for bacterial and viral infections of the eye The risk can be minimized by the use of individual eyecups Eyeglass eyepiece with Eyepiece for users who do not Fixed eyepiece with inclined adjustable diopter and straight wear eyeglasses with adjustable eyecup eyecups diopter and inclined eyecup Leica E Series Manual Dioptric Correction Only for Leica EZ4 Open Version Dioptric Correction The adjustable eyepieces for the Leica EZA are also available with built in diop tric correction allowing the microscope to be used without glasses even by those with vision problems The correction comprises a maxi mum of 5 diopter settings Leica E Series Users with normal vision do not need adjus table eyepieces An adjustable eyepiece is sufficient for mild shortsightedness If vision is extremely poor or when graticules are used two adjustable eyepieces are needed When the diopters are regulated exactly the sharpness is constant over the entire magnifi cation range parfocal Once the diopters have been set the procedure does not have to be repeated Simply restore your diopter values on the eyepiece before beginning work with the stereomicroscope Manual Adjusting ie Set the dioptric correction for both eyepie ces to 0 Select the lowest magnification and focus on a flat specimen Se
15. e camera is in PC mode when the LED status display is illuminated in red and flashes green approximately once per second Capturing Images without a Computer in HD Mode Continued Capturing an image 2 Press the red image capture button briefly 1 Insertthe SD memory card into the SD card You hear an audible signal as confirmation slot until you hear it click into position and the camera LED flashes green The LED illuminates in green The camera is now You will hear another beep when the Leica ready to save images to the SD card ICC50 HD has completed capturing the image The image is now saved on the SD card Leica E Series Manual High resolution Video Display without a Computer HD mode Cables A The HDMI cable required for this purpose 1 Connect the camera and the HDMI device is included in the standard delivery of the via the integrated HDMI port Leica EZ4 HD A Live images and captured images can be output in high resolution via the HDMI cable e Full HD 1920x1080 pixels e HD ready 1280x720 pixels e Movie 720x480 pixels Leica E Series Manual Changing the Resolution The output resolution Full HD or HD ready is configured directly on the microscope 1 Press a pin into the pinhole switch in order to change the resolution PC Mode Use with a Computer For instructions on operating the instru ment in PC mode refer to the DVD inclu ded in the standard delivery of the Leica EZ4 HD The
16. ed camera module Light distribution Optical 50 Camera 50 Internal video magnification 0 5x Sensor Centered to optical axis 0 1 mm Hoizontally Vertically aligned 1 degree Electronic interfaces Leica E Series USB 2 0 mini USB plug Mini HDMI HD ready and Full HD LED with 2 colors green ready red image capture Integrated slot SD Secure Digital card SD card 1 8 GB WIFI WORM support High Capacity HC Black multifunction button for changing the illumination mode press for 1 second and for switching between HD and PC mode press and hold for 5 seconds Red multifunction button for image capture using SD card press for 1 second configuring a new white balance press and hold for 5 seconds reset camera press and hold for 10 seconds Other e Power supply integrated power supply e Power requirement 4 W e Operating temperature range 5 C to 50 C e Relative humidity 10 to 90 e CE Declaration of Conformity Available e Tested standards EMI RFI EN55011 B EN50082 1 e EMC 61000 3 2 61000 3 3 Manual Dimensions Leica ES2 e ES s Manual Leica E Series Leica ES2 Continued Leica EZ4 HD PRI gt lt Ss e Pe R E gt Leica E Series Leica EZ4 HD Continued IPD 50 to 75 Leica EZ4 10x Eyepieces n E pe v Y wi o v Pa Leica EZ4 10x Eyepieces Continued IPD 50 to 75 itl e UY
17. ed if the WB ambient light so that color neutral images can be acquired MODE setting is set to AUTO A If the WB MODE is set to AUTO the white balance is carried out continuously This mode is recommended only for color neutral specimens and those stained over a wide area Leica E Series Manual COLOR Manual White Balance gt COLOR SET WHITE BAL PRESS OK WB MODE MANUAL RESOLUTION RED LEVEL E gt SETUP CAMERA BLUE LEVEL gt SETUP USER BLACK LEVEL o gt MAIN MENU SUBMENU 1 Leica E Series The COLOR function makes it possible to adapt the camera chip to the ambient light so that color neutral images can be acquired Manual Adjusting the white balance manually recommended 1 Move the specimen out of the field of view so that only the illumina tion is seen button on the remote control Press the Call up the COLOR entry Select the SET WHITE BAL menu item and press the OK button If necessary correct the values for RED and BLUE until the image shows a uniform gray area Press the amp button to exit the menu EXPOSURE Manual exposure COLOR EXP MODE MANUAL 1 Press the amp button on the remote control NK 2 Call up the command for manual exposure see diagram at the left ERIGHENESS ni d 3 Set the value for EXP MODE to MANUAL MAIN MENU SUBMENU 4 Comeet the Males for EXP
18. eo Playback fullscreen view 2 Play back individual image press 2x e Previous next image lt or e Playback video C9 e Fast forward reverse or Thumbnail view Leica E Series ETE Calling up the Camera Menu The remote control does not work if the Opening and closing the camera menu i camera is connected to a computer and 1 Point the remote control towards the is in PC mode The menus of the camera are microscope also no longer displayed on the monitor In this case press the black multifunction button for 2 Press the key on the remote control to 5 seconds to switch to HD mode refer also to show the camera menus on the monitor page 42 3 Press the A w 4 and gt buttons to select a menu item 4 Press the OK button to confirm a menu item 5 Press the camera menus button again to hide the Leica E Series VETTE COLOR Automatic White Balance Enabling automatic white balance gt COLOR SET WHITE BAL PRESS OK 1 Press the amp button on the remote control WB MODE AUTO La a 2 Call up the COLOR entry SETUPICAMERA PUP REYES a d 3 Set the value for WB MODE to AUTO SETUP USER BLACK LEVEL o gt RESOLUTION RED LEVEL MAIN MENU SUBMENU 4 Press the amp button to exit the menu Gi The COLOR function makes it possible to adapt the camera to the The values for RED and BLUE cannot be adjust
19. er Bo a little while it will become automatic Leica E Series Manual Using the LED illuminator Iluminating the specimen Tips on use 1 Position a specimen in the middle of the e Use incident light for plastic opaque baseplate objects 2 Switch the two LED illuminators on or off as e Use transmitted light for transmitted light desired specimens or transparent objects e Use the combination of both LED illumina tors for partially transparent objects A After switching off the LEDs remain lit for about another 4 seconds Leica E Series Manual Focusing A Focusing raises and lowers the stereomi Focusing croscope using the focusing drive The 1 Setthe magnification changer to the lowest specimen is brought into sharp focus as soon as level 10 it is in the focal point of the objective The focusing drive can be operated either left or right handed 2 Set the working distance to approx 100 mm for coarse focusing S Focusing drive torque Leica E Series Manual 3 Set the magnification changer to the second level 30 4 Use the focusing drive once again for fine focus After you have adjusted the image sharp 1 ness at high magnification it is maintai ned even if you switch to a lower magnification parfocal Magnification Display You can read the configured magnifica i tion on the right hand rotary knob of the Leica ES2 The additional magnification provi ded by t
20. ge 12 Eyepieces and Eyecups Only for Leica EZ4 Open Version 31 The Correct Interpupillary Distance 13 Dioptric Correction Only for Leica EZ4 Open Version 32 Using the LED illuminator 14 Graticules Only for Leica EZ4 Open Version 34 Focusing 15 Graticules Preparation 35 Magnification Display 16 Inserting the Graticules 36 Adjusting the Resistance of the Focus Drive 17 Photography Using the Leica EZ4 HD Leica EZ4 HD Overview of the instrument 38 Overview Leica EZ4 19 General Instructions 39 Instrument variant Leica EZ4 HD 20 Introduction 40 Transport Placement and Storage 21 Standard delivery 41 The Correct Interpupillary Distance 22 HD mode and PC mode 42 Switching on the LED Illuminator 23 Capturing Images without a Computer in HD Mode 43 Controlling the Automatic Shutoff 24 High resolution Video Display without a Computer HD mode 45 Illumination types 25 PC Mode Use with a Computer 46 Focusing 26 PC Mode Limitations 47 Magnification Display 27 PC Mode Additional Use of a HDMI Device 48 Adjusting the Resistance of the Focus Drive 28 Configuring the White Balance 49 Restoring Factory Settings 50 Leica E Series Manual Contents Continued he Camera Menu Optional Remote Control 52 Viewing Images and Movies with the Optional Remote Control 53 Calling up the Camera Menu 54 COLOR Automatic White Balance 55 COLOR Manual White Balance 56 EXPOSURE 57 RES
21. he eyepieces is already incorporated in the scale Leica E Series Manual Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focusing drive too easy or too difficult to adjust No problem the resistance can be adjusted individually depending on your perso nal preferences 1 Grip the drive knobs with both hands and turn them towards each other until the desired resistance is reached during focu sing Leica E Series Manual Leica EZ4 and Leica EZ4 HD Overview Leica EZ4 10x fixed eyepieces for eyeglass wearers Carrying handle Magnification changer Focusing drive Integrated LED incident light illumination Illumination control Integrated LED transmitted illumination Leica E Series Manual Instrument variant Leica EZ4 HD 10x fixed eyepieces for eyeglass wearers Integrated digital camera with SD card slot Carrying handle Magnification changer IR receiver for optional remote control Focusing drive Integrated LED incident light illumination Illumination control Integrated LED transmitted illumination Leica E Series Manual Transport Placement and Storage Built in carrying device Setting up the microscope Packing the device away All Leica E Series microscopes are equip 1 Place the microscope on a flat tabletop 1 After use wind up the cable as shown Li ped with a holder for convenient and in the illustration safe transport 2 Connect the p
22. he risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups Leica ES2 Overview Leica ES2 10x fixed eyepieces for eyeglass wearers Carrying handle Two level magnification changer 10x and 30x Focusing drive Integrated LED incident light illumination Integrated LED transmitted illumination Switch for incident and transmitted light NAUA UNS Leica E Series Manual Transport Placement and Storage Built in carrying device Setting up the microscope Packing the device away All Leica E Series microscopes are equip 1 Place the microscope on a flat tabletop 1 After use wind up the cable as shown Li ped with a carrying handle for conveni in the illustration ent and safe transport 2 Connect the power supply to a grounded socket 2 Store the instrument so that it is protected against dust Leica E Series Manual The Correct Interpupillary Distance The Ideal Image Note for eyeglass wearers Setting the eye distance The interpupillary distance is correctly If you wear glasses fold the eyecups back 1 Lookinto the eyepieces Li set if you see a single circular image field Li otherwise fold the eyecups forwards when looking at a specimen 2 Hold the eyepieces with both hands Push the eyepieces together or separate them until you see a circular image You may need a short time to become familiar SY with using the instrument Not to worry aft
23. lect the highest magnification and read just the sharpness Select the lowest magnification again but do not look into the eyepieces Carry out steps 6 to 8 for each objective individually Rotate the eyepiece counterclockwise in the direction as far as it will go 5 diop ter settings Dioptric Correction Continued 7 Lookinto the eyepiece 8 Slowly rotate the eyepiece individually in the direction until the eye sees the object sharply imaged 9 Select the highest magnification and refo cus if necessary Now if you adjust the magnification from the highest to the lowest level the specimen is always brought into sharp focus If not repeat the process Leica E Series Manual Graticules Only for Leica EZ4 Open Version Graticules Available eyepiece inserts The transparent graticules can be inser 3 Graticules 10 mm 0 1 mm ted in the adjustable eyepieces of the Leica EZ4 They enable easy measurements at the specimen 5 mm 0 05 mm 100 Div 0 002 100 Div 0 001 150 Div 0 0005 5 mm 0 1 mm i For equipment with a graticule in one eyepiece two adjustable eyepieces are needed Since one eye is focussed on the stage micrometers 50 mm 0 1 0 01 mm graduation eyepiece with the graticule it needs a second 1 0 001 graduation adjustable eyepiece so that it can be aligned Crosshairs Crosshairs optically with the first Leica E
24. ot so wet that solvent runs over the lens Protect the microscope from moisture fumes and acids and from alkaline caustic and corrosive materials Do not store chemicals in the vicinity Protect the microscope from oil and grease Never grease or oil mechanical parts or sliding surfaces It is advisable to enter a service agreement with Leica Service Manual microscope There is a handle on the back of the microscope and an undercut in the front for this purpose Specifications Camera Module Weight 700 g camera only Height 50mm Exposure time 2 msec 2 sec Live image Max 45 fps 1256 x 720 px 20 fps 1920 x1080 px Full frame image acquisition 2048 x 1536 pixels 3 1 megapixels Movie clip 720 x 480 pixels MP4 Sensor size 6 55 mm x 4 92 mm Pixel size 3 2 um x 3 2 um Sensor grade Micron 1 2 CMOS Gain 1x to 20x Color Depth 24 bit Data format JPEG TIFF BMP MP4 Operating systems Windows XP Windows Vista Windows 7 Mac OS X Software available LAS EZ software PC optional LAS modules Acquire Mac Min computer config Intel Pentium 4 or DuoCore 2 GHz 2 GB RAM 24 bit graphics 1248 x 1024 DVD Min display specification HD ready 1280x720 Full HD 1920x1080 e Standard HDMI port Connection via DVI using a HDMI DVI adapter is not recommended ETT Camera Module Continued Mechanical and optical interfaces Integrat
25. ower supply to a grounded socket 2 Store the instrument so that it is protected against dust Leica E Series Manual The Correct Interpupillary Distance The Ideal Image Note for eyeglass wearers Setting the eye distance The interpupillary distance is correctly If you wear glasses fold the eyecups back 1 Lookinto the eyepieces Li set if you see a single circular image field Li otherwise fold the eyecups forwards when looking at a specimen 2 Hold the eyepieces with both hands Push the eyepieces together or separate them until you see a circular image You may need a short time to become familiar SY with using the instrument Not to worry after Bo a little while it will become automatic Leica E Series Manual Switching on the LED Illuminator Automatic shutoff 2 Switch the two LED illuminators on or off as For a detailed description of the illumina If the illumination is not operated for 60 desired l tor refer to page 25 minutes it switches off automatically The illumination can be reactivated by pressing a button on the membrane keypad LED illumination 1 Position a specimen in the middle of the baseplate e Use incident light for plastic opaque objects e Use transmitted light for transmitted light specimens or transparent objects e Use the combination of both LED illumina tors for partially transparent objects Leica E Series Manual Controlling the Autom
26. r more than 5 sec until a message appears on the screen Manual SETUP USER User defined Settings COLOR SET ILLUM ILLUM 1 MENUCOLOR LEICA LAS RESOLUTION SHOW MENU 15 SEC SETUP CAMERA SHOW CAPTURE 2 SEC gt SETUP USER LANGUAGE ENGLISH Main menu Submenu A The user defined settings enable you to for example save illumina tion scenarios and retrieve them later SET ILLUM 1 to 3 save illumination modes These settings allow you to store up to three different illumination modes and retrieve them later Select the number of the illumination mode and press OK to store the current camera settings for later acquisition situ ations Leica E Series SET MENUCOLOR Currently two color schemes are available for the menu guidance Additi onal colors may be provided in the future via a firmware update SHOW CAPTURE Defines how long an image is displayed on the screen after being captu red SHOW MENU Defines how long the menu is displayed on the screen SET LANGUAGE Select the language of the menu Manual Predefined Illumination Scenarios A The camera in the Leica EZ4 HD provides various illumination modes that cover a wide variety of possible situations Changing the illumination mode 1 Press the black multifunction button briefly to display the current illumination mode on the monitor or TV set Leica E Series 2 Press the button again to switch to the next illumina
27. stall any other plug NEMA 5 15P or unscrew any mechanical components unless expressly instructed to do so in the instructions The microscope must be connected to a grounded outlet Always position the microscope so that A you can disconnect it from the power supply at any time The power cable is provided as the power disconnect device Instructions for Use Continued Intervention from the user The microscopes of the E Series contain Li no components that can be maintained or repaired by the user Exceptions are menti oned specifically in this manual Unless otherwise specified in this manual repair and service work may be carried out by authorized Leica technicians only Unauthorized alterations to the instru ment or noncompliant use shall void all rights to any warranty claims Leica E Series Maintenance Leica E Series microscopes are basically maintenance free To ensure that it always operates safely and reliably we recom mend that you take the precaution of contac ting the responsible service organization Here you can arrange for periodic inspections and conclude a maintenance contract recommen ded Accessories Only those accessories may be used that are listed in this user manual or for which safe use has been confirmed by Leica Microsystems Manual Danger of infection Direct contact with eyepieces is a poten A tial transmission method for bacterial and viral infections of the eye T
28. tion mode 3 Press the red image capture button or wait until the menu no longer appears To return to the last selected mode press l the black multifunction button seve ral times in succession until the LAST USED setting appears A If you do not press the black multifunc tion button for 5 seconds the selected illumination mode is taken over Manual LAST USED y AUTO y BRIGHTFIELD y DARKFIELD y ILLUMINATION 1 y ILLUMINATION 2 y ILLUMINATION 3 Care and Transport Care and Transport General Instructions e Avoid excessive use of solvents The lint e Keeping all optical components clean is free cloth lens cloth or cotton swab should Transport Always use both hands to carry the important for maintaining good optical performance e Always protect the microscope and camera with the plastic cover when the instru ment is not in use Included in the delivery package of the microscope Be sure power to the camera is off when the dust cover is in place If any optical surface becomes badly coated with dust or dirt it should be cleaned by blowing the surface with a syringe or brus hing it off with a camel hair brush before attempting to wipe the surface clean Optical surfaces should be cleaned using a lint free cloth lens cloth or cotton swab moistened with a commercially available glass cleaner Do not use alcohol Leica E Series be soaked with solvent but n
29. to be completed in which the televi sion and monitor can even be used together Thus you are ideally prepared for every situa tion work at the computer presentations trai ning sessions trade show appearances mate rial testing and more User friendly even in the smallest detail Like every digital camera that of the Leica EZ4 HD reacts differently to different light sources However the white balance is factory set to match the LED illumination of the microscope This ensures that you obtain the best results automatically Manual Standard delivery DVD with Leica software USB cable HDMI cable drivers and manuals Please do not discard any of the packing materials They should be used for safely storing and transporting the instrument should the need arise Leica E Series Manual SD card Optional Remote control with battery Type CR2025 HD mode and PC mode The camera is normally in what is known as HD mode after being switched on In this mode it outputs a high resolution signal in 16 9 wide screen format The images can then be saved directly to an SD card If the Leica EZ4 HD is connected to a computer via USB and the Leica software provided is star ted the camera switches automatically to what is called PC mode In this mode images are transmitted to the computer with a size of up to 3 megapixels The aspect ratio is always 4 3 Leica E Series A If the camera is in PC mode images c
30. using the Leica software Leica E Series 4 3 aspect ratio Manual 16 9 aspect ratio PC Mode Additional Use of a HDMI Device A In PC mode the camera image is always A To use a higher resolution on the connec Connecting the HDMI monitor shown in the preview window of the ted screen select HD mode on the 1 Connect the HDMI cable provided to the Leica software In addition another computer camera camera monitor or television can be connected via the HDMI interface 1 To switch between PC and HD mode press and hold the black multifunction button for The image resolution on the attached 5 seconds HDMI or television screen is limited to the live image resolution inside the LAS EZ software Due to technical constraints it is not possible to send a live image stream to the connected screen in HD ready or Full HD quality 2 Connect the HDMI cable to the HDMI televi sion or monitor Leica E Series Manual Configuring the White Balance When configuring the white balance of l the camera make sure that no specimen is in the beam path and that a standard illumi nation is available Configuring a New White Balance Press and hold the red exposure button for 5 seconds Wait for the beep As soon as the signal sounds a new white balance has been configured Leica E Series Manual We recommend carrying out a new white balance adjustment whene ver you change the illumination type or
31. w these inst ructions may pose hazards to personnel cause instrument malfunctions or damage the instru ment Important information This symbol indicates additional infor mation or explanations that intend to provide clarity Manual EC Declaration of Conformity Electrically operated accessories are construc ted based on the state of the art of technology and are provided with an EC Declaration of Conformity Contact address Leica Microsystems Switzerland Ltd Industry Division Max Schmidheiny Strasse 201 CH 9435 Heerbrugg Switzerland Instructions for Use Intended use The E Series microscopes are optical instru ments for improving the visibility of objects through magnification and illumination It is used for observing and documenting Place of use Use the instrument in enclosed dust free rooms at 10 C to 40 C only Protect it from oil chemicals and extreme humidity It must be at least 10 cm from the wall and away from flammable subs tances Leica E Series Interference Avoid large temperature fluctuations direct sunlight and vibrations These conditions can distort measurements and micrographic images In warm and warm damp climatic zones Li the individual components require special care in order to prevent the build up of fungus Use in clean rooms Leica E Series microscopes can be used in clean rooms without any problems Manual Handling electrical components Never in
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