Dynex OPSYS MW 91000051 User's Manual
Contents
1. Select option MOVE PURGE gt Opsys MW User s Guide 53 Creating a New Wash Protocol To specify a Soak outside of a Move ee Note A Soak can be performed either within or outside of a Move cycle The procedure below describes the procedure for specifying a soak outside of a Move cycle The procedure for specifying a Soak within a Move cycle is described on page 47 1 Scroll right to display the next selection The display will read Select option lt 4 SOAK END 2 Select SOAK The display will read Duration 5 3 Enter the desired soak duration 1 to 999 seconds using the keypad er Note A soak duration ranging from 1 to 999 seconds can be entered 4 Press ENTER The display will read Select option lt SOAK END 54 Opsys MW User s Guide Creating a New Wash Protocol To specify the End of the wash protocol 1 Scroll right to display the next selection The display will read Select option lt SOAK END 2 Select END The display will read 1 NAME 01 SETUP PROGRAM Opsys MW User s Guide 55 Modifying a Wash Protocol An existing wash protocol can be modified by using many of the same commands that are used to create a wash protocol To modify a wash protocol 1 Display the main menu 1 NAME 01 SETUP PROGRAM 2 Use the up and down scroll keys to select the wash protocol to be modified o2. The CAL command allows the calibration values for each pump to be specified 1 Select SETUP if needed The display will read Select option HEAD PLATE gt 2 Scroll right to display the next selection Select option lt 4 VERSION CAL gt 3 Select the pump pressure high or low The display will read Pump speed 190 4 Enter the desired value using the keypad and press ENTER The display will read Slope 2 1 er Note The Slope and Intercept values should only be changed by DYNEX Technologies Service Personnel 5 Press ENTER to keep the existing value The display will read Intercept 24 6 Press ENTER to keep the existing value The display will again read Select option lt VERSION CAL gt Note Refer to page 74 for additional pump calibration instructions 36 Opsys MW User s Guide Configuring the System Selecting Clean The CLEAN command enables the washer to be cleaned automatically at a specified interval 1 Scroll right to display the next section Select option lt CLEAN 2 Select CLEAN The display will read Auto Clean YES NO 3 Select YES if you want periodic automatic cleaning The display will read Interval Hrs 1 4 Enter the desired interval using the up down scroll keys and press ENTER er Note The minimum interval is 1 hour and the maximum i
3. 3 Fill out an Equipment in Transit form Figure 10 4 Pack the Washer and the Equipment in Transit form for shipment 5 After you receive a return authorization ship the Washer to the nearest DYNEX Technologies facility see page 85 Opsys MW User s Guide 83 Returning a Washer for Service Prater EQUIPMENT IN TRANSIT IMPORTANT Please include a copy of this form with each instrument If your instrument contains a hard drive please retain back up copies of any stored files Failure to do so may result in the loss of those files Return Authorization Number Contact Technical Service DYNEX Technologies phone 800 288 2354 or 703 631 7800 ext 1440 fax 703 631 7816 Equipment Serial Number EQUIPMENT DECLARATION Clearly indicate fault condition or reason for return CERTIFICATE OF DECONTAMINATION I certify that the equipment described above has been disinfected decontaminated and is clean dry and fit for transport Signed Title Date DYNEX Technologies reserves the right to refuse improperly cleaned equipment Shipping Address DYNEX Technologies Inc Attn Above return number 14340 Sullyfield Circle Chantilly VA 20151 1683 Suggested decontamination methods Readers Wash all surfaces with a 10 Hypochlorite solution Follow that with a mild detergent solution Washers Please follow the Decontamination Procedure found in the back of the manual Figure 10 Equipment in
4. soak within a Move cycle The procedure for specifying a Soak outside of a Move cycle is described on page 50 1 Scroll right to display the next selection The display will read Select option lt 4 SOAK FILL gt 2 Select SOAK The display will read Duration 5 3 Enter the desired soak duration using the keypad ee Note A soak duration ranging from 1 to 999 seconds can be entered 4 Press ENTER The display will read Select option lt SOAK FILL gt Opsys MW User s Guide 51 Creating a New Wash Protocol 52 To specify a Fill within a Move er Note A Fill must be performed within a Move cycle 1 Scroll right to display the next selection The display will read Select option lt SOAK FILL gt 2 Select FILL The display will read Fluid volume 50 3 Use the keypad to enter the volume of fluid to be dispensed during well filling Note A volume ranging from 50 to 300 microliters can be entered 4 Press ENTER The display will read Select option lt SOAK FILL gt Opsys MW User s Guide Creating a New Wash Protocol Ending a MOVE Cycle 1 Scroll right to display the next selection The display will read Select option lt SOAK FILL gt 2 Scroll right to display the next selection The display will read Select option lt END LOOP 3 Select END LOOP The display will read
5. 1 The waste container contains the fluid that is aspirated from the wells A level sensor is contained inside of the waste container Opsys MW User s Guide Hardware Components Connectors at the rear of the Opsys MW system are shown in Figure 3 Connections for the power cord dispense pump power waste bottle level sensor waste bottle vacuum line and an external computer are made at the rear of the system s mportant Be sure that the vacuum exhaust is not obstructed VACUUM EXHAUST DISPENSE PUMP DISPENSE POWER CONNECTOR TRAP VACUUM TUBING HYDROPHOBIC FILTER WASTE LEVEL SENSOR CONNECTOR UPS 110 240 Figure 3 Rear View of the Opsys MW System Opsys MW User s Guide 17 Positioning the Instrument Positioning the Instrument Determine the area where the system will be located You will need a firm and level area that is approximately 51 cm 20 inches wide 62 cm 24 inches deep and 40 cm 16 inches high for the Opsys MW 18 Opsys MW User s Guide Installing the Wash Head Installing the Wash Head The Wash Head must be located on the Wash Head Arm To install the Wash Head 1 Position the Arm over the Purge Reservoir portion of the Plate Holder 2 Place the Wash Head on the two Arm Pins being careful to properly seat the Wash Head on the support pins 3 Level the Wash Head in relation to the Plate Holder and t
6. 2 Wash Head 3 Wash Head Positions 4 Wash Fluid 5 Waste 5 Features 7 Specifications 9 Warning Labels 11 Chapter 2 Installation 13 Unpacking 13 Materials Provided 13 Hardware Components 15 Positioning the Instrument 18 Installing the Wash Head 19 Installing the Wash Container 20 Installing the Waste Container 21 Turning on the System 22 Chapter 3 Configuration 23 The Keypad 23 Menus 26 The SETUP Menu 27 The PROGRAM Menu 27 Configuring the System 28 Specifying the Wash Head 29 Specifying Plate Parameters 30 Displaying the Software Version 35 Specifying the Pump Parameters 36 Selecting Clean 37 Chapter 4 Wash Protocols 39 Wash Protocol Operations 39 Creating a New Wash Protocol 41 Specifying the Header 41 Specifying Wash Cycle Operations 45 Ending a MOVE Cycle 53 Modifying a Wash Protocol 56 Copying a Wash Protocol 61 Chapter 5 Washing a Plate 63 Preparation 63 Washing a Plate 65 Chapter 6 Routine Maintenance 67 Routine Maintenance Procedures 67 Opsys MW User s Guide i Table of Contents Cleaning and Decontamination 69 Cleaning the Containers 70 Replacing the Tubing 71 Pump Calibration 74 Chapter 7 Troubleshooting 77 Operational Problems 77 Chapter 8 Service 81 Service Procedures 81 Spare Parts 82 Returning a Washer for Service 83 Contact DYNEX Technologies 85 Index 86 ii Opsys MW User s Guide About this Manual About this Manual This manual has been written for laboratory technicians and provides
7. Cap of the Waste Container and lay it with the level sensor assembly on a clean paper towel Discard the contents of the Waste Container in accordance with local regulations Thoroughly rinse the container with deionized water and let it dry Disconnect the waste tubing from the connector on the Waste Container cap and from the waste tubing connector on the wash head Remove the waste tubing from the instrument Obtain a new length of waste tubing Route the waste tubing through the lower slot at the front corner of the instrument and through the routing clips at the front of the instrument Connect one end of the waste tubing to the waste tubing connector on the wash head and connect the other end to the connector in the Waste Container cap Place the level sensor assembly back into the Waste Container and screw the Cap on Opsys MW User s Guide 73 Pump Calibration Pump Calibration Each Opsys MW washer manifold or head e g 1 x 8 1 x 12 has three calibration values stored in memory These values are calculated and stored during manufacture at DYNEX Technologies When the Opsys MW configuration is changed from one manifold to another the corresponding calibration settings will be recalled by correctly setting the head size using the SETUP HEAD feature refer to page 28 for Specifying the Wash Head This calibration protocol may be used at any time to verify the performance of the Opsys MW washer IMPORTANT It i
8. Transit Form 84 Opsys MW User s Guide Contact DYNEX Technologies Contact DYNEX Technologies DYNEX Technologies 14340 Sullyfield Circle Chantilly VA 20151 USA Tel 703 631 7800 Toll Free 800 288 2354 Fax 703 803 1441 www dynextechnologies com Opsys MW User s Guide 85 Index Index as required maintenance 68 ASPIRATE 39 aspiration height 4 entering 32 bottom wash specifying 34 bottom wash height 4 CAL command 36 calibration prototcol 74 cleaning 69 Wash Container 70 Waste Container 70 configuration 28 connections 17 daily maintenance 67 data bits 9 decontamination 69 DISPENSE 39 dispense height 4 entering 32 dispense pump 20 display panel 15 emptying Waste 70 End Loop 47 error messages 77 FILL 39 fluid levels 63 foam aerosol trap 21 footprint 18 function keys 24 hardware components 15 HEAD command 29 keypad 16 23 Keypad 9 main menu 22 maintenance 67 as required 67 daily 67 six month 67 weekly 67 MOVE 39 47 name changing 57 Opsys MW 86 description 1 features 7 illustration 1 overview 1 programming 2 specifications 9 parity 9 password 41 56 plate carrier 15 plate number displaying 31 plate parameters 30 specifying 31 positioning 18 power ON 22 Printer 9 problems 77 PROGRAM 41 Program menu 26 27 protocol number displaying 42 pump parameters 36 PURGE 39 45 46 replacing tubing 71 return of a wa
9. Warranty When installation by DYNEX Technologies personnel is not required the period of this Warranty shall begin on the date of shipment from DYNEX Technologies The period of this Warranty shall begin as described above whether or not the product has been installed pursuant to a purchase order and any trial period shall be deducted from the Warranty period that would otherwise apply under a subsequent placed purchase order for that product Limitation of Liability Notwithstanding anything to the contrary contained herein the liability of Seller whether by reason of breach of warranty breach of contract tort or otherwise including without limitation under any indemnification provision contained herein shall be limited to replacement of goods returned to DYNEX Technologies which are shown to DYNEX Technologies reasonable satisfaction to have been nonconforming or to refund the purchase price or if not paid to a credit amount of the purchase price therefore THE FOREGOING WARRANTIES ARE EXCLUSIVE AND ARE GIVEN AND ACCEPTED IN LIEU OF ANY AND ALL OTHER WARRANTIES EXPRESS OR IMPLIED INCLUDING WITHOUT LIMITATION THE IMPLIED WARRANTY OF MECHANTABILITY AND THE IMPLIED WARRANTY OF FITNESS FOR A PARTICULAR PURPOSE NEITHER PARTY SHALL BE LIABLE TO THE OTHER FOR ANY INCIDENTAL INDIRECT SPECIAL OR CONSEQUENTIAL DAMAGES Table of Contents Table of Contents About this Manual Chapter 1 Introduction 1 Description 1 Programming
10. and dispense pins are free from potential salt build up Opsys MW User s Guide Configuring the System Specifying the Wash Head The HEAD command allows the installed wash head to be specified 1 Select SETUP The display will read Select option HEAD PLATE gt 2 Select HEAD The current wash head selection is displayed in the upper line Wash head 8 8 gt If needed scroll right to view the remaining selection lt Wash head 8 lt 12 3 Select the desired option using the function key After the selection is made the display will again read Select option HEAD PLATE gt Opsys MW User s Guide 29 Configuring the System Specifying Plate Parameters The PLATE command allows plate parameters to be specified for up to 10 plate types When setting plate parameters a default value is displayed for each of the wash head positions The default values are factory set and represent approximate starting values for each wash head position S Note The wash head positions are described on page 4 Increasing the value of a wash head position by one unit lowers the head 0 1 mm and decreasing the numerical value of a wash head position by one unit raises the head 0 1 mm e g an increment of 20 steps equals one millimeter of vertical pin travel 20 1mm up 20 1mm down The SHOW command is available when setting each wash head position Select SHOW to
11. detailed instructions for using the Opsys MW system This manual gives you the information needed to e Review safety precautions e Install the Oosys MW system e Understand the Oosys MW menus e Set the Opsys MW system up to suit your exact needs e Create or modify wash protocols e Run wash protocols using the Opsys MW system e Perform required preventive maintenance e Troubleshoot the system Opsys MW User s Guide iii About this Manual This page is intentionally left blank iv Opsys MW User s Guide Description Chapter 1 Introduction The Opsys MW is a microprocessor controlled microplate washing system that performs wash protocols that are defined by the user It is designed to wash all of the wells in one column or one row of a 96 well plate at once The washing protocol can be programmed so that all of the columns or rows are washed in the same manner or different wash cycles can be applied to specified columns or rows on the plate Description The Opsys MW Microplate Washer is shown in Figure 1 ASPIRATE TUBING DISPENSE TUBING Figure 1 The Opsys MW Microplate Washing System Opsys MW User s Guide 1 Description Programming All wash protocols on the Opsys MW Microplate Washer are user defined Up to 40 wash protocols can be contained on the system ee Note Memory limitations may restrict the size and co
12. name up to 9 characters keypad Enter an optional password NOTE If the password for a protocol is lost the ability to access the method and the method position are also lost 4 Aorv or use keypad Select the plate type 4 Specify whether the operator will be prompted at run time to enter the number of strips to be washed avoids reprogramming the number of strips Variable strips 4 Constant timing If Variable strips is YES Specify whether the time for washing any number of strips on a plate is constant Figure 8 Specifying the Wash Protocol Header After the wash protocol header information is defined the specific steps in the wash protocol are specified The procedures for defining the wash protocol header are described in the following pages The procedures for specifying the steps in a wash protocol are described beginning on page 45 Opsys MW User s Guide 41 Creating a New Wash Protocol To create a new wash protocol header 1 42 Display the main menu Besessseose SETUP PROGRAM Use the up or down scroll keys to display the protocol number or use the keypad to enter the protocol number that is to be created lees ea eee SETUP PROGRAM Se Note Dashes will be displayed if a protocol has not yet been named Select PROGRAM The name of the wash protocol is displayed in the lower line Enter name Use
13. on the total number of strips entered at run time e g the protocol states Move 3 12 1 cycle s and at run time 2 strips are entered only strips 3 and 4 will be processed If YES is selected the display will read Constant timing YES NO er er Note If NO is selected for Variable strips the Select option prompt will be displayed see below Note The Constant Timing prompt only appears if you are using Variable Strips Opsys MW User s Guide 43 Creating a New Wash Protocol 44 10 Select YES if the total elapsed time for washing any number of strips within a plate is to be constant The display will read Select option MOVE PURGE gt The sequential steps in the wash protocol can now be specified as outlined on page 45 Opsys MW User s Guide Creating a New Wash Protocol Specifying Wash Cycle Operations The final step in creating a wash protocol is to specify individual operations within the wash protocol An example of a wash protocol is shown in 45 In this example the dispense wash pins are purged and then a Dispense and Aspirate are carried out twice on each of the 12 strips columns of a 96 well Microtiter plate Finally after all 12 strips have been washed twice the contents of the Microtiter plate are allowed to soak equilibrate for another 600 seconds PURGE Purge the dispense pins 4 MOVE Specify the starti
14. speed using the SETUP CAL function refer to page 36 e A pump speed change of 10 units roughly corresponds to a well volume change of 10ul 9 Ifthe dispensed volume is too low increase the pump speed using the SETUP CAL function refer to page 36 e A pump speed change of 10 units roughly corresponds to a well volume change of 1 Oul 10 Repeat Steps 3 9 until fluid is dispensed with the accuracy required e g Error less than or equal to 5 Opsys MW User s Guide 75 Pump Calibration This page is intentionally left blank 76 Opsys MW User s Guide Operational Problems Chapter 7 Troubleshooting Operational Problems Problems that may occur during normal operation of the Opsys MW are described below For each problem the symptom probable cause and the means for resolving the problem are shown Symptom Probable Cause Resolution The instrument The cord is not Check that the cord is fails to power up connected plugged into the instrument and into the electrical outlet Verify that the electrical outlet is not controlled by a switch or timer Verify that there is power at the electrical outlet by plugging in and checking another device The powerstrip is Replace the powerstrip faulty The fuse is Service is required blown The breaker is Service is required tripped The power supply Reset the breaker is defective Liquid is not The dispense Remove the wash head dispensed fr
15. the Waste Container cap 3 Route the waste tubing through the lower slot at the front corner of the instrument and through the routing clips at the front of the instrument 4 Connect the end of the waste tubing to the waste tubing connector on the wash head 5 Connect the waste level sensor cable to the waste level sensor connector at the rear of the instrument Figure 3 Opsys MW User s Guide 21 Turning on the System Turning on the System S Note Depending upon local electrical codes an uninterruptible power system UPS may be required in your laboratory A UPS is not provided with the Opsys MW system 1 Connect the Opsys MW to the laboratory electrical supply outlet er CAUTION The Opsys MW instrument must be connected to properly grounded electrical outlets Obtain assistance from a qualified electrician to verify that your electrical outlets are properly grounded Before connecting the power cable be sure that the components have been connected to each other as outlined in the previous section 2 Push the instrument power switch once After a series of self tests the software version is displayed momentarily Opsys MW v1 0 before the main menu is displayed eeeoeoece SETUP PROGRAM er 22 Note The self test results indicate whether the system is operating properly If a self test failure is reported it is recommended that you call Technical Service Das
16. upper slot in the front corner of the instrument and through the routing clips at the front of the instrument Fit the dispense tubing in the dispense valve 10 Fill the Wash Container with fresh wash fluid Place the Wash Container Internal Tube and pick up filter back into the Wash Container and screw the Cap on Reconnect the dispense line quick connector Opsys MW User s Guide 71 Replacing the Tubing To replace the vacuum tubing S Note This procedure describes replacement of the vacuum tubing connecting the Waste Container to the Foam Aerosol Trap The vacuum tubing connecting the Foam Aerosol Trap to the rear of the instrument should not require changing 1 Unscrew the Cap of the Waste Container lay it and the level sensor assembly on a clean paper towel 2 Discard the contents of the Waste Container in accordance with local regulations Thoroughly rinse the container with deionized water and let it dry 3 Disconnect the vacuum tubing from the connector in the Cap and from the connector in the Foam Aerosol Trap Discard the old tube 4 Obtain a new length of vacuum tubing 5 Connect one end of the vacuum tubing to the connector in the Waste Container Cap and the other end to the connector in the Foam Aerosol Trap 6 Place the level sensor assembly back into the Waste Container and screw the Cap on 72 Opsys MW User s Guide Replacing the Tubing To replace the waste tubing 1 Unscrew the
17. PROGRAM menu contains commands to name and create a new wash protocol or to edit an existing wash protocol 1 TESTNAME SETUP PROGRAM a v Enter name If selected protocol has E E Mee eee not been named or Edit name If selected protocol has 4 WASHNAME oa ana hasng or If selected protocol has Enter password been named and nasa password Figure 6 PROGRAM Menu Opsys MW User s Guide 27 Configuring the System Configuring the System The Opsys MW must be configured before it is operated for the first time The commands for doing this are contained in the SETUP menu Figure 7 Use ARROW keys to scroll through the menus 1 TESTNAME SETUP PROGRAM A Select Option HEAD PLATE gt Select Option lt VERSION CAL gt Figure 7 SETUP Menu The purpose of each command on the SETUP menu is summarised below Command HEAD PLATE VERSION CAL CLEAN 28 Purpose Specify the type of head that is fitted Displays the PLATE menu The commands on this menu allow parameters such as well top height dispense height and wash height for various plate types to be specified Displays the version number of the software Displays the CAL menu The commands on this menu allow the pump parameters to be specified The CLEAN command enables the washer to be cleaned automatically at a specified interval This feature assists in assuring that tubing
18. Unscrew the cap of the Wash Container and lay it and the Pick Up Filter on a clean paper towel 3 Fill the Wash Container with the wash fluid that is being used er Note If the wash solution is being changed discard the contents of the Wash Container and thoroughly clean it before filling it with the new wash solution 4 Place the Pick Up Filter into the Wash Container and screw the cap on Re connect the dispense line quick connector Opsys MW User s Guide Washing a Plate Washing a Plate A plate is washed by positioning it on the carrier block selecting the wash protocol to be run and pressing the START key To wash a plate 1 If needed Turn on the instrument The display will read 1 TESTNAME SETUP PROGRAM 2 Place the microplate on the plate carrier ee Note Be sure that the plate position on the carrier plate corresponds to the wash head configuration that is being used For example ifa 1 xX 8 head is used be sure that the plate is positioned with the columns aligned with the wash head rather than perpendicular to the wash head 3 Select the desired wash program using the up or down scroll keys or by entering the wash protocol number using the keypad er Note Before starting the wash protocol verify that the correct wash protocol name is displayed 4 Press the START key The wash program will be automatically carried out ee Note If variable strips was specified
19. across the bottom of the well during aspiration to better remove all droplets of liquid If NO is selected the Wash height prompt Step 14 will be displayed Note Use the sweep function with Flat or C bottom plates only Sweeping aspirates fluid more completely from the wells than a regular aspiration Sweep all cycles to get the most thorough well aspiration when using multiple aspiration cycles but remember that each sweep adds seconds to the protocol Opsys MW User s Guide Configuring the System Remember if your protocol requires sweeping that must first be specified in the plate definition used in the protocol 11 Select YES to specify a sweep on all aspiration cycles The display will read Sweep height 370 SHOW Note The default value is displayed Select SHOW to move the wash head to the default sweep position so that the height can be checked Then determine the correct value as outlined on page 30 Repeat the procedure as needed 12 Enter the desired value using the keypad and press ENTER The display will read Sweep stroke 100 amp Note There isn t a SHOW function for the sweep stroke Determine optimum sweep stroke setting by starting with a small number and increase it if the residual aspirate volume is not acceptable If you run a protocol and the wash pins strike the sides or bottom of the well reduce the sweep stroke and review your plate setup para
20. asher The wash fluid is contained in the Wash Container and is dispensed into the wells whenever a Dispense or Fill operation is specified in the wash protocol In addition wash fluid is dispensed into the purge tray during a Purge operation Dispensing of the fluid from the Wash Container is controlled by a pump attached to the back of the Opsys MW Microplate Washer and a dispense valve located near the wash head Figure 1 Waste Fluid is removed from the wells during an Aspirate or Dispense operation and collected in the Waste Container The Waste Container contains a level sensor to alert the operator when the container is full Removal of the fluid from wells is controlled by lowering the wash head so that the aspiration pins are immersed into the well fluid the aspiration height A vacuum is applied to the aspiration pins at all times during operation so that the contents of a well are aspirated whenever the wash head is lowered to the aspiration height Opsys MW User s Guide 5 Description This page is intentionally left blank 6 Opsys MW User s Guide Features Features The Opsys MW system has a number of performance and convenience features These are summarised below e Small footprint e Upto 40 wash protocols can be programmed e Up to ten plate types can be programmed e Two wash head configurations 1 x 8 and 1 x 12 e On board self diagnostics e SHOW function allows visual verification of wa
21. be contaminated by overflow Opsys MW User s Guide 79 Symptom Aspiration is not complete continued High Waste Level is displayed The waste container is deformed X Motor Home Error is displayed Z Motor Home Error is displayed Test Too Large is displayed Probable Cause Hydrophobic filter may be obstructed The Waste Container is full The waste level sensor cable is not plugged in The waste level sensor connector is defective The waste level sensor detector is reversed The waste tubing is blocked The waste container is damaged or defective Motion of the wash head is being blocked The motor is defective Motion of the wash head is being blocked The motor is defective The wash protocol exceeds the memory limit Operational Problems Resolution Replace filter Empty the Waste Container see page 70 Connect the waste level sensor cable see page 17 Service is required Service is required Remove the waste tubing from the rear of the instrument see page 17 Check the waste tubing and aspiration tubing and remove any blockages or kinks Replace the waste container Check the wash head vicinity and remove any obstacles Service is required Check the wash head vicinity and remove any obstacles Service is required Reduce the number of steps in the wash protocol and try again Opsys MW User s Guide S
22. e Refer to page 23 for instructions on entering alphabetic characters 6 Press ENTER The display will read DISP HEIGHT 180 SHOW Note The default value is displayed Select SHOW to move the wash head to the default dispense position so that the height can be checked Then determine the correct value as outlined on page 30 Repeat the procedure as needed Opsys MW User s Guide 31 Configuring the System 32 7 Enter the dispense height using the keypad and press ENTER The display will read Welltop 160 SHOW S Note The default value is displayed Select SHOW to move the wash head to the default well top position so that the height can be checked Then determine the correct value as outlined on page 30 Repeat the procedure as needed 8 Enter the desired value using the keypad and press ENTER The display will read ASP HEIGHT 380 SHOW S Note The default value is displayed Select SHOW to move the wash head to the default aspiration position so that the height can be checked Then determine the correct value as outlined on page 30 Repeat the procedure as needed 9 Enter the desired value using the keypad and press ENTER The display will read Sweep NO YES NO 10 Select YES to specify a sweep The display will read All cycles NO YES NO S Note If a sweep is specified the wash pins are moved up and down and
23. ervice Procedures Chapter 8 Service Service Procedures The only user service procedures that can be carried out for the Opsys MW are listed below e Removal and replacement of the wash head assembly e Cleaning of the wash head assembly To remove the wash head assembly 1 Loosen the two thumbscrews at the front of the Arm Head assembly 2 Grasp the wash head assembly and lift it up from the retaining pins on the Arm 3 Lift the wash head and tubing clear of the instrument If necessary remove the wash tubing and waste tubing from the retainer clips at the front of the instrument To clean the wash head assembly 1 Pass the Cleaning Wire through the inside of each wash pin and waste pin on the wash head 2 Run a PURGE to rinse any material from the wash pins To replace the wash head assembly 1 Position the wash head assembly back on the Arm pins 2 If necessary replace the wash tubing and waste tubing into the retainer clips at the front of the instrument Opsys MW User s Guide 81 Spare Parts Accessories 13001580 Plate Holder Assembly 13001610 8 Way Head Assembly 13001630 12 Way Head Assembly 352104000 Cleaning Wire 0 040 352101800 Cleaning Wire 0 018 91000051 User Manual 91000070 OPSYS MW Quick Set Up Instructions 23501800 Bottle Wide Mouth 2120 0010 Nalgene Wide Mouth Bottle w Cap 029006501 Jar Aerosol Trap 029027400 Bottle Waste 4L w Modified Top 200074300 Rack Bo
24. for the wash protocol you will be prompted to enter the strips that are to be washed before the wash will commence Opsys MW User s Guide 65 Washing a Plate This page is intentionally left blank 66 Opsys MW User s Guide Routine Maintenance Procedures Chapter 6 Routine Maintenance Routine Maintenance Procedures The Opsys MW Microplate Washer is constructed from materials that resist chemical attack If a spill occurs it should be cleaned up as soon as possible using a cloth moistened with a mild laboratory detergent If needed dilute the laboratory detergent according to the manufacturer s directions The following periodic maintenance procedures are required for the Opsys MW Daily maintenance e Verify that the self test passes by observing Arm movement and the absence of error messages e Clean the plate carrier using a moist towel e Clean the external surfaces using a moist towel Weekly maintenance e Empty the Wash Container and clean it The wash solution should be replaced at least once a week The Wash Bottle should be cleaned whenever fresh wash solution is required S Note If particulate matter is observed in the Wash Bottle the wash solution should be discarded at once To replace the wash solution 1 Discard any wash solution that is left in the Wash Bottle 2 Rinse the Wash Bottle with fresh solution 3 Fill the Wash Bottle with wash solution 4 Purge the sys
25. hes are initially displayed after the wash protocol number i e 1 in the above example if a wash protocol has not yet been named Opsys MW User s Guide The Keypad Chapter 3 Configuration The Keypad The keypad is used to access the menus and commands for setting up the Opsys MW entering new wash protocols and running the wash protocols The keypad contains scroll keys function keys and alphanumeric keys The keys on the keypad are shown in Figure 4 Scroll Keys Display Panel Function Keys Scroll Keys Alphanumeric Keys Figure 4 Keys on the Keypad Opsys MW User s Guide 23 The Keypad The use of each of the keys is summarized below Key Purpose Scroll keys The functionality of the scroll keys depends upon what is being displayed During display of the main menu page 26 pressing the up or down scroll keys will display successive wash protocol numbers and names or dashes if a protocol is not yet named Horizontal scrolls will scroll through selections for the selected menu Repeatedly pressing the horizontal scroll button will show the next two items in alist A list with one item will have a blank entry If you are entering or editing information a scroll will move the cursor through edit fields When editing an existing plate or protocol name you may either type over the existing name or press the DEL key to delete the characters from the existing name NOTE To delete the enti
26. igereerst Opsys MW Microplate Washer Users Guide IMPORTANT Please read carefully before using the Opsys MW Part No 91000051 Revision B This manual is published by DYNEX Technologies Inc Questions or comments regarding the content of this manual can be directed to the address below or to your DYNEX Technologies representative DYNEX Technologies 14340 Sullyfield Circle Chantilly VA 20151 1683 USA Tel 703 631 7800 800 288 2354 Fax 703 803 1441 OPSYS MW is a trademark of DYNEX Technologies Inc 2006 This document is the copyright of DYNEX Technologies and must not be copied or reproduced in any form without prior consent DYNEX Technologies reserves the right to make technical improvements to this equipment and documentation without prior notice as part of a continuous program of product development This manual supersedes all previous editions Warranty and Special Provisions Limited Warranty DYNEX Technologies products are fully guaranteed for one year against defects in parts materials and workmanship Defective parts and materials will be replaced or at the discretion of DYNEX Technologies repaired at no charge for a period of one year and labor required for such replacement or repair will be provided at no charge for a period of one year provided that the products are utilized and maintained in accordance with the instructions in the applicable operating and servicing manual and provided f
27. ighten the two thumbscrews at the front of the Wash Head Arm Assembly Opsys MW User s Guide 19 Faksiecliltare Mm takown Aves tlamOxe autsllatsya Installing the Wash Container The dispense tubing and dispense pump power cable must be connected To install the Wash Container alts O 20 Fill the Wash Container with the wash solution that is to be used S Note The Wash Container contains up to 4 liters Insert the Pick Up Filter into the Wash Container and secure the cap Attach the Quick Connector at the end of the pump tubing to the mating connector in the cap Route the dispense tubing through the upper slot at the front corner of the instrument and through the routing clips at the front of the instrument Connect the end of the dispense tubing to the wash tubing connector on the wash head Thread the dispense tubing through the dispense valve Figure 1 Note The tubing must be inserted fully into the dispense valve opening to ensure proper operation Connect the dispense pump power cable to the dispense pump power connector at the rear of the instrument Figure 3 Opsys MW User s Guide AcM Muarown Ales like Oxe aitslialsya Installing the Waste Container To install the Waste Container and Foam Aerosol Trap 1 Insert the Quick Connect end of the vacuum tubing on the Foam Aerosol Trap to the Quick Connect vacuum connector at the rear of the instrument Figure 3 2 Screw on
28. ill read Start strip 1 Using the keypad enter the strip number where the move cycle should start Press ENTER The display will read End strip 12 Using the keypad enter the strip number where the move cycle should end Press ENTER The display will read Cycles 1 Using the keypad enter the number of the specified move cycles that are to be sequentially performed er Note Between 1 and 9 Move cycles can be specified Press ENTER The display will read Select option ASPIRATE DISPENSE gt Specify Aspirate Dispense Soak and Fill operations in the sequence as they are to be carried out as described in the following sections When all of the operations for the Move cycle have been sequentially specified specify End Loop to designate the end of the Move cycle Opsys MW User s Guide 47 Creating a New Wash Protocol 48 To specify an Aspiration within a Move er Note An Aspirate or Dispense must be performed within a Move cycle 1 Select ASPIRATE The display will read Cycles 1 2 Using the keypad enter the number of aspiration cycles that are to be to be performed within the MOVE cycle 3 Press ENTER The display will read Select option ASPIRATE DISPENSE gt Opsys MW User s Guide Creating a New Wash Protocol To specify a Dispense within a Move er Note A Dispense or Aspirate must be performed with
29. in a Move cycle 1 Select DISPENSE The display will read Fluid volume 350 2 Use the keypad to enter the volume of fluid to be dispensed Note A volume ranging from 50 to 999 microliters can be entered 3 Press ENTER If a bottom wash is specified for the plate being used the display will read Bottom Wash NO YES NO eS Note If the protocol requires bottom washing that must first be specified in the plate definition used in the protocol If a bottom wash is not specified for the plate being used or if NO is selected for the Bottom Wash prompt the display will read Select option ASPIRATE DISPENSE gt 4 Select YES if a bottom wash is to be included The display will read Fluid volume 350 5 Use the keypad to enter the volume of fluid to be dispensed during the bottom wash Note A volume ranging from 50 to 999 microliters can be entered Opsys MW User s Guide 49 Creating a New Wash Protocol 6 Press ENTER The display will read Cycles 1 7 Use the keypad to enter the number of bottom wash cycles to be performed and press ENTER The display will read Select option ASPIRATE DISPENSE gt 50 Opsys MW User s Guide Creating a New Wash Protocol To specify a Soak within a Move er Note A Soak can be performed either within or outside of a Move cycle The procedure below describes the procedure for specifying a
30. ing the wash head assembly allows the well contents to be aspirated or a bottom wash to be performed Raising the wash head assembly allows the plate to be moved so another column or row can be washed or so the wells can be filled Opsys MW User s Guide 3 Description Wash Head Positions The vertical positions that the wash head can assume are described below Each wash head position can be specified by the user to within 0 1 mm er Note Procedures for specifying wash head positions for various plate types are described on page 30 Wash Head Position Description Dispense Height Positions the aspiration pins slightly below the top of the well so that fluid can be dispensed into the well up to a certain level before it is aspirated Well Top Positions the aspiration pins so they are aligned with the top of the well Aspiration Height Positions the aspiration pins at the bottom of the well so that the contents of the well can be completely aspirated Sweep Height Raises the aspiration pins slightly above the Aspiration Height see above so that the aspiration pins can be moved back and forth in the well while the fluid is being aspirated Bottom Wash Height In protocols with a bottom wash lowers the wash head during dispense so that the force of the dispensed fluid can wash the bottom of the wells 4 Opsys MW User s Guide Description Wash Fluid A single wash fluid is used on the Oosys MW Microplate W
31. ispense Height Standards The instrument is designed in accordance with CSA 1010 1 92 UL 3101 1 EN61010 1 10 and EN61326 Up to 10 characters Up to 8 characters 1 8 for 1 x 8 heads and 1 12 for 1 x 12 heads Increment 1 1 8 for 1 x 8 heads and 1 12 for 1 x 12 heads Increment 1 1 9 Increment 1 1 9 Increment 1 50 999 Increment 1 uL 50 9999 Increment 1 uL 50 300 Increment 1 uL 1 999 Increment 1 second Up to 10 characters 001 999 Increment e 001 999 Increment e 001 999 Increment e 001 999 Increment e 001 999 Increment 001 999 Increment e Opsys MW User s Guide Warning Labels Warning Labels The Opsys MW or its components may contain certain labels that that either warn the user of a hazard or note an electrical connection The labels that may be used on the Opsys MW system are described below Label Description Alternating current is present English Caution symbol Refer to the Routine Maintenance chapter French Attention voir documents d accompagnement English Caution hot surface French Attention surface chaude Protective conductor terminal Earth ground terminal English Caution risk of electric shock gt H 0e Pi French Attention risque de choc lectrique Opsys MW User s Guide 11 Warning Labe
32. ks Reseat the tubing in the dispense valve Service is required Insert the dispense tubing into the dispense valve see page 20 Opsys MW User s Guide Operational Problems Symptom Probable Cause Resolution Dispense volumes The dispense Check the pins seals and are incorrect pins are leaking tubing for leaks The dispense Check the seals and pressure is low tubing for leaks Check for air leaks Reseat the tubing in the dispense valve The dispense Remove the wash head pins are clogged assembly and clean it using the manifold head cleaning wire Then runa purge Service is required if problem persists Incorrect Recalibrate Pump Calibration Aspiration is not The aspiration Remove the wash head complete pins are clogged assembly and clean it using the manifold head cleaning wire Service is required if problem persists The aspiration pin Repeat the plate setup height is set too procedure page 30 high The seal on the Check the seal and waste waste container is tubing for leaks leaking The wash If the bottoms of the plate protocol is not wells are flat use a optimized sweep Add additional aspiration cycles to the wash protocol Aspiration tubing Check the aspiration may be blocked tubing and remove any or kinked blockages or kinks The vacuum Remove any obstructions exhaust may be at the vacuum exhaust blocked see page 17 The vacuum Service is required pump may
33. ls This page is intentionally left blank 12 Opsys MW User s Guide packing o Chapter 2 Installation Unpacking Materials Provided The main carton contains the instrument The contents of this carton are listed below Article Quantity Opsys MW System Instrument Power Cable Cleaning Wire Aspirate Cleaning Wire Dispense Instrument Configuration Report User s Guide Declaration of Conformity Wash Head 1 x 8 or 1 x12 Plate Holder PO er Se Co a A een Coen A sub carton contains the wash container and waste container assemblies The contents of this carton are listed below Article Quantity Wash Container Assembly 1 Waste Container Assembly 1 Opsys MW User s Guide 13 14 To unpack the components 1 Place the Opsys MW instrument in the position where it will be located for use Place the Wash Container Waste Container and Foam Aerosol Trap at the rear of the instrument Examine the packaging to be sure that the power cord and other materials have been removed Please save packaging material for future use Verify that all of the materials listed above have been unpacked Inspect the components for damage If damage is observed contact your shipper or service representative immediately Opsys MW User s Guide Hardware Components Hardware Components Hardware components of the Opsys MW instrument are shown in Figure 2 and described below e Note The Wash Contai
34. ly clean it before filling it with the new wash solution Place the Pick Up Filter into the Wash Container and screw the cap on Reconnect the dispense line quick connector Opsys MW User s Guide Replacing the Tubing To replace the dispense tubing 1 Separate the dispense line quick connector in the cap of the Wash Container Unscrew the cap lay it and the Pick Up Filter on a clean paper towel 2 Discard the contents of the Wash Container Thoroughly rinse the container with deionized water and let it dry 3 Disconnect and discard the Wash Container Internal Tube and pick up filter that is attached to the barb on the Quick Connect Fitting in the Cap 4 Replace the Wash Container Internal Tube and pick up filter 5 Remove the Quick Connect Fitting from the Pump Tube that is attached to the Pump Inlet Disconnect the tube from the pump and discard 6 Reattach the Quick Connect Fitting to a new length of Pump Tube and attach the free end of the tube to the Pump Inlet 7 Disconnect both ends of the Dispense Tube that is attached to the Pump Outlet and the Wash Head remove the tubing from the dispense valve and discard 8 Attach the short length of larger diameter tube to the Pump Outlet and to the other end fit the larger barb of the Reducer Fitting To the small end of the Reducer fit the long length of small diameter dispense tube and attach the free end to the Wash Head 9 Route the dispense tubing through the
35. meters 13 Enter the desired value using the keypad and press ENTER The display will read Bottom wash NO YES NO 14 Select YES if a bottom wash is to be included The display will read Wash height 368 SHOW ee Note The default value for bottom wash height is displayed Select SHOW to move the wash head to the default bottom wash position so that the height can be checked Then determine the correct value as outlined on page 30 Repeat the procedure as needed Opsys MW User s Guide 33 Configuring the System 15 Enter the desired value using the keypad and press ENTER The display will read Select option HEAD PLATE gt er Note If NO is selected for Bottom wash Step 13 the display will read as shown above 34 Opsys MW User s Guide Configuring the System Displaying the Software Version The VERSION command displays the software version that is currently installed on the Opsys MW instrument 1 Select SETUP if needed The display will read Select option HEAD PLATE gt 2 Scroll right to display the next selection Select option lt 4 VERSION CAL gt 3 Select VERSION The display will momentarily read Opsys MW v1 0 ee Note The software version that is displayed will depend on the version installed in the instrument Opsys MW User s Guide 35 Configuring the System Specifying the Pump Parameters
36. move the wash head to the indicated position so that the height can be observed For example when setting dispense height the default value is 180 DISP HEIGHT 180 SHOW If after selecting SHOW the dispense pins are found to be too low by approximately 1 0 mm the dispense height position can be raised by 1 0 mm by entering 170 for the dispense height When using the SHOW function when defining the aspirate height if the aspirate pins touch the well bottom increase the aspirate height If you cannot move the plate slightly side to side and up down without immediate interference from the aspirate pins increase the aspirate height 30 Opsys MW User s Guide Configuring the System To specify parameters for a plate type 1 Obtain an empty plate of the plate type being specified and place it in the plate holder 2 Select SETUP if needed The display will read Select option HEAD PLATE gt 3 Select PLATE The name of the first plate is displayed in the lower line Select plate Ipeseeosoose Ee Note Dashes will be displayed if the plate has not yet been named 4 If needed display a different plate number using the up or down scroll keys or use the keypad to enter the desired plate number When the desired plate number is displayed press ENTER The display will read Enter name 5 Use the keypad to enter the plate name up to 10 characters er Not
37. mplexity of an individual wash protocol In addition the system can be configured with up to ten different plate types so that the wash head positions for each plate type can be specified Programming of a new wash protocol or modifying an existing one and configuration of the system are carried out using the keypad and display 2 Opsys MW User s Guide Description Wash Head The wash head on the Opsys MW Microplate Washer contains multiple sets of dual wash pins Each set of dual wash pins consists of a dispense pin and an aspirate pin that are closely spaced so that fluid can be aspirated from and dispensed into a well simultaneously The following wash heads can be fitted on the Opsys MW Wash Head Description 1x8 Eight sets of dual wash pins for washing a 96 well microplate one column 8 wells at a time 1x12 Twelve sets of dual wash pins for washing a 96 well microplate one row 12 wells at a time If a wash head is installed to wash plates by columns the plates are placed on the instrument so that the columns of the plate are aligned with the wash head If a wash head is installed to wash plates by row the plate is rotated 90 degrees and placed on the instrument so that the rows of the plate are aligned with the wash head The wash pins are fixed to the wash head During operation the wash head assembly is lowered to insert the wash pins into the wells or raised to remove the wash pins from the wells Lower
38. ner Waste Container and Foam Aerosol Trap are external to the instrument and are not shown in Figure 2 Wash Head Microtiter Plate Plate Carrier Display Panel Keypad Figure 2 The Opsys MW System e Wash Head The wash head contains sets 1 x 8 or 1 x 12 of dual wash pins Each set of wash pins consists of a dispense pin and an aspirate pin closely spaced so that fluid can be aspirated from a well while fluid is being dispensed into the same well The wash head moves back and forth to wash each row or column depending on the wash head that is used of the plate by lowering the wash pins into the corresponding wells e Plate Carrier The plate carrier holds the plate in a fixed position so that the wash pins can be precisely lowered to the correct height and position in the wells during the washing procedure e Display Panel The display panel is the means by which the system communicates to the user All messages commands and wash parameters entered by the user are displayed Opsys MW User s Guide 15 Hardware Components 16 Keypad The keypad is used by the operator to select commands and enter wash parameters RS232 Interface The system includes an RS232 interface so that an external computer can be connected for technical service purposes Wash Container Shown in Figure 1 The wash container contains the wash fluid that is used for Dispense Fill and Purge operations Waste Container Shown in Figure
39. ng strip and ending strip for each cycle 2 Cycles and the number of cycles to be performed 4 DISPENSE 3 Times For each strip perform dispense three times ASPIRATE For each strip perform one aspiration cycle END LOOP End the cycle v SOAK After the two Move cycles have been completed allow the contents to equilibrate for 600 seconds END End the wash protocol Figure 9 Example of a Wash Protocol The procedures for specifying the steps in this and other wash protocols are described on the following pages Opsys MW User s Guide 45 Creating a New Wash Protocol 46 To specify a Purge er Note A purge must be performed outside of a Move cycle 1 Display the header information for the selected wash protocol as outlined on page 41 The display will read Select option MOVE PURGE gt 2 Select PURGE The display will read Fluid volume 350 3 Use the keypad to enter the volume of fluid to be dispensed er Note A purge volume ranging from 50 to 9 999 microliters can be entered 4 Press ENTER The display will read Select option MOVE PURGE gt Opsys MW User s Guide Creating a New Wash Protocol To specify a Move cycle 1 Display the header information for the selected wash protocol as outlined on page 41 The display will read Select option MOVE PURGE gt Select MOVE The display w
40. nterval is 9 hours The display will read Fluid Volume 2500 5 Enter the desired volume of fluid to be dispensed ee Note A cleaning volume ranging from 50 to 9 999 microliters can be entered Follow the instructions on page Error Bookmark not defined for refilling the wash container 6 Press ENTER The display will read Select option lt CLEAN Opsys MW User s Guide 37 Configuring the System This page is intentionally left blank 38 Opsys MW User s Guide Wash Protocol Operations Chapter 4 Wash Protocols Wash Protocol Operations A wash protocol consists of a series of Purge Move Soak Aspirate Dispense and Fill operations Purge Move and Soak can be carried out in any sequence Aspirate Dispense and Fill can only be carried out within a Move operation and there cannot be a Move within a Move Each of these operations is described below Operation Description Purge Dispenses fluid from the dispense wash pins while the wash head is positioned over the purge tray Note Purge is usually carried out at the beginning of a wash protocol to rinse the dispense wash pins and remove air bubbles Move Performs Aspirate Dispense Fill and or Soak operations on specified strips of the Microplate Aspirate Removes the contents of a well by positioning the wash pins at the aspiration height in the well and aspirating the liquid from the wells A sweep may al
41. omthe pins are clogged assembly and clean it dispense pins using the manifold head cleaning wire Then runa purge Service is required if problem persists The Wash Refill the Wash Container Container is empty Opsys MW User s Guide 77 Symptom Liquid is not dispensed from the dispense pins continued Liquid is dispensed continuously from the dispense pins Probable Cause The wash protocol is incorrectly defined The dispense tubing or the dispense pump power supply is not connected The dispense tubing is sealed shut at the dispense valve The dispense valve does not open The wash head manifold tubing is blocked There isa pressure leak The dispense pump is faulty The dispense tubing is not inserted in the dispense valve Operational Problems Resolution Check the system configuration and wash protocol for the number fluids and fluid volume Verify that the dispense tubing and the dispense power supply are connected see page 20 Remove the tubing from the dispense valve and massage the tubing to release it Replace the tubing into the dispense valve at a different location Purge the tubing more frequently Replace the tubing Service is required Remove the wash head assembly and clean it using the manifold head cleaning wire Then run a purge Service is required if problem persists Check the seals and tubing for leaks Check for air lea
42. otocol number that is displayed represents the first free protocol number if any are available Opsys MW User s Guide 61 Copying a Wash Protocol 7 Using the keypad enter the number of the wash protocol that the commands are to be copied to If a wash protocol already exists at that location the display will read Overwrite test NO YES NO 8 Select YES The display will read 7 NAME_04 SETUP PROGRAM 62 Opsys MW User s Guide Preparation Chapter 5 Washing a Plate Once the parameters for at least one plate type and one wash protocol have been set the system can be used to wash a plate Preparation The only preparation that is required is to check the fluid levels in the Wash Container and the Waste Container Service the containers if necessary To empty the waste container 1 Unscrew the cap of the Waste Container Lay the cap and level sensor assembly on a paper towel 2 Discard the waste in accordance with local regulations 3 Rinse the waste container with deionized water Discard the rinse water amp Note If desired the waste container can be disinfected with a 10 v v solution of household bleach in water 4 Place the level sensor and tube back into the Waste Container and screw the cap on Opsys MW User s Guide 63 Preparation 64 To refill the wash container 1 Separate the dispense line quick connector in the cap of the Wash Container 2
43. r enter the number using the keypad The display will read 4 NAME 04 SETUP PROGRAM 3 Select PROGRAM If the wash protocol is password protected the display will read Enter password er Note If a Password is not specified for the selected wash protocol the display will read as shown in Step 5 4 Enter the Password using the keypad and press ENTER er Note If the Password is entered incorrectly an error message is displayed The operator is allowed three attempts to enter the password After the third attempt the main menu Step 1 will again be displayed Note If the password for a protocol is lost the ability to access the method and the method position are also lost 5 After a password is successfully entered or if a password was not specified for the selected protocol the display will read Edit name 4 NAME_04 COPY 56 Opsys MW User s Guide Modifying a Wash Protocol 6 The cursor is positioned under the first character of the name If the name is to be changed enter the name using the keypad and press ENTER The first step of the wash protocol will be displayed MOVE 1 12 1 Cycles DELETE EDIT gt S Note The example above shows the MOVE operation as the first step in the protocol The actual operation that is shown will depend upon the protocol that is being modified Note For each step that is displayed the operation being carried out and
44. ration that the new operation is to precede is displayed MOVE 1 10 lt INSERT 1 Cycles er Note The inserted operation will take place immediately before the operation that is displayed 2 Select INSERT The display will read Select option MOVE PURGE gt 3 Select the option that is to be inserted using the same procedure as when creating a new wash protocol Opsys MW User s Guide Copying a Wash Protocol An existing wash protocol can be copied to another program number If a wash protocol is already in that location it will be overwritten To copy a wash protocol 1 Display the main menu 1 NAME_01 SETUP PROGRAM 2 Use the up and down scroll keys to select the wash protocol to be modified or enter the protocol number using the keypad The display will read 4 NAME_04 SETUP PROGRAM 3 Select PROGRAM If the wash protocol is password protected the display will read Enter password 4 Enter the Password using the keypad and press ENTER ee Note If the Password is entered incorrectly an error message is displayed The operator is allowed three attempts to enter the password After the third attempt the main menu will be displayed 5 After a password is successfully entered the display will read Edit name 4 NAME_04 COPY 6 Select COPY The display will read Copy to 7 ee Note The pr
45. re name either type over it or press the right arrow key and DEL to delete all characters to the right of the cursor The first character of an existing name cannot be deleted but it can be overwritten Function keys Two function keys select the item or execute the command shown directly above each key on the second line of the display Del key When a modifiable entry is displayed selecting Delete will allow the entry to be modified While editing a field selecting Delete removes the character at the cursor location and all characters to the right of the cursor Enter key Completes an entry of characters accepts default prompt data and displays the next prompt in a series of commands Start key Starts a wash protocol This can only be done while the main menu is being displayed Cancel key Returns to the previous menu within the function 24 Opsys MW User s Guide The Keypad Key Purpose The numeral shown on the key is displayed by pressing the key once If the key is pressed two to seven times rapidly one of the letters on the key will be displayed The letter that is displayed depends upon the number of times the key is pressed Alphanumeric Keys Opsys MW User s Guide 25 Menus The Opsys MW menus are hierarchical and are organised by function There are two main menus Setup and Program Menu Purpose SETUP Configure the Instrument PROGRAM Create or edit plate washing p
46. rotocols A protocol includes general information e g the type of microplate to be washed as well as a listing of the various steps to be used in the washing process Once the instrument is switched on and the self test is successfully completed the main menu is displayed It contains SETUP and PROGRAM menu headers le Soo SETUP PROGRAM Note Dashes are initially displayed after the wash protocol number i e 1 in the above example if a wash protocol has not yet been named Once a wash protocol is named the name is displayed after the protocol number Refer to page 41 for the procedure to create and name a wash protocol Display a different wash protocol number and name there can be up to 40 by pressing the up or down scroll keys or by entering the protocol number using the keypad Access a menu by pressing the function key beneath the menu header Cancel a menu selection and display the previous higher level menu by pressing the CANCEL key 26 Opsys MW User s Guide The SETUP Menu Press the function key under SETUP to display the SETUP menu Figure 5 The SETUP menu contains commands to configure the instrument 1 TESTNAME SETUP PROGRAM A v Select Option HEAD PLATE gt Select Option lt VERSION CAL gt Figure 5 SETUP Menu The PROGRAM Menu Press the function key under PROGRAM to display the PROGRAM menu Figure 6 The
47. s recommended that this protocol be completed if any fluid carrying component of the Opsys MW is replaced This protocol may also be used to recalibrate the Opsys MW if a new wash buffer with different viscosity is used Customize the protocol to emulate your assay using the appropriate dispense volume pump speed and fluid density Calibration Protocol 1 Define a flat bottom plate profile using the SETUP PLATE function refer to page 30 for Specifying Plate Parameters 2 Program an assay to dispense 200ul well into columns 1 12 refer to page 41 for Creating a New Wash Protocol 3 Obtain a clean dry flat bottom 96 well styrene plate 4 Weigh the plate and record the empty plate weight in grams 5 Run the assay programmed in Step 2 This assay will dispense 200u l to all 96 wells in the plate 6 Weigh the plate and record the filled plate weight in grams 7 Calculate the actual dispensed volume and error using the following formulas A Actual Volume Full weight Empty weight x 1000mg g 96 wells x fluid density B Error 100 x Actual Volume Target Volume Target Volume Example Empty plate weight 48 38g Full plate weight 67 42g Density of water at 20 C 0 998mg ul Actual Volume 67 42 48 38 x 1000 96 x 0 998 198 7ul well Error 100 x 198 7 200 200 0 6 74 Opsys MW User s Guide Pump Calibration 8 Ifthe dispensed volume is too high decrease the pump
48. sh head height settings e High level alarm on Waste Container Opsys MW User s Guide 7 KEWEY This page is intentionally left blank 8 Opsys MW User s Guide Specifications Dimensions and Weight Length Width Height Weight Operation Dispense Range Dispense Precision Residual Aspirate Volumes Washing Time Power Display Keypad Output Environmental Operating Range Computer Interface Port Baud Rate Character Format Power Requirements Main Unit Line Voltage Variation Line Frequency Variation 49 cm 19 3 in 38 cm 15 0 in 19 cm 7 5 in 9 1 kg 20 Ibs 50 999 uL 5 CV with 300 uL distilled water lt 1 uL for U and V bottom plates lt 5 uL for flat bottom plates Typical 5 cycle wash 5 min 30 sec lt 100 W 2 by 20 character back lit LCD 21 key tactile membrane Display Printer RS232 15 C to 30 C 15 to 85 relative humidity non condensing 2000m altitude Serial RS 232 port Service Access 19200 Character format 7 data bits 1 stop bit no parity Voltage Power Frequency 100 240 V 200 VA 50 60 Hz 10 3 Hz Opsys MW User s Guide Input Ranges Assay Programming Assay Name Password Move Start Strip Move End Strip Move Cycles Aspirate Cycles Dispense Purge Fill Soak Plate Setup Plate Name Dispense Height Top of Well Height Aspirate Height Sweep Height Sweep Stroke Bottom Wash D
49. sher 83 RS232 9 16 scroll keys 24 Setup menu 26 28 Setup Menu 27 SHOW command 30 six month maintenance 68 SOAK 39 software version 35 spare parts 82 stop bits 9 sweep entering 32 sweep height 4 DYNEX Technologies Contacts 85 timing 41 troubleshooting 77 tubing 71 usage area 18 vacuum tubing 72 Opsys MW User s Guide Po dex CERS variable strips 41 warning labels 11 Wash Container 5 16 cleaning 64 installing 20 refilling 64 wash fluid 5 wash head 3 15 cleaning 81 installing 19 positions 4 removal 81 replacement 81 types 3 wash pins 3 wash pins 3 Opsys MW User s Guide wash protocol copying 61 deleting 59 inserting 60 modifying 56 operations 39 45 wash protocols programming 2 washing a plate 63 65 waste 5 Waste Container 16 cleaning 63 emptying 63 waste tubing 73 weekly maintenance 67 well top 4 87
50. so be performed during Aspirate Note The aspirate height and sweep parameters for a particular plate type are specified during system configuration 30 If a sweep is specified during configuration the wash pins are moved back and forth during the aspiration cycle Opsys MW User s Guide 39 Wash Protocol Operations Operation Description Dispense Dispenses a specified amount of fluid into the wells after aspirating the contents of the wells i e wherever a DISPENSE step is added to a protocol the OPSYS MW will automatically aspirate the wells before dispensing more fluid in order to prevent a well overflow If a bottom wash is specified the wash head is then lowered to the bottom wash position so that fluid will be aspirated from the bottom of the wells while fluid is being dispensed Note The dispense height for a particular plate type is specified during system configuration page 30 Soak The contents of the wells are allowed to equilibrate for the specified number of seconds Fill The wells are filled with a specified amount of fluid 40 Opsys MW User s Guide Creating a New Wash Protocol Specifying the Header NOTE Pressing CANCEL while creating or editing a protocol will bring you back to the main menu without saving your changes Aorv Select one of the 40 protocol numbers or use keypad Mw PROGRAM Select PROGRAM from the main menu w keypad Enter a protocol
51. tem to ensure that all of the old solution is removed from the tubing e Empty the Waste Container and clean it e Inspect the tubing between the Wash Bottle and the Wash Head e Inspect the tubing between the Wash Head and the Waste Bottle e The tubing should be replaced if it has cracks is cloudy remains pinched or is otherwise defective Six month maintenance Opsys MW User s Guide 67 Routine Maintenance Procedures e Replace the dispense Wash tubing e Replace the aspirate Waste tubing S Note The dispense tubing and aspiration tubing may need to be replaced more frequently than every six months depending upon the frequency of use and the severity of operating conditions Contact DYNEX Technologies for information on replacement tubing e Replace the in line hydrophobic filter If the system will not be used for a period of time the system should be flushed with de ionized water so that buffers and or reagents used to wash the plates are not deposited on the Wash Head or other components of the system As required maintenance e Pump Calibration page 74 68 Opsys MW User s Guide Cleaning and Decontamination The Opsys MW is constructed from materials that resist chemical attack Spills should be cleaned as soon as possible If you need to decontaminate the Opsys MW instrument for example before servicing the instrument clean the system and then decontaminate it as described belo
52. the keypad to enter the protocol name up to 9 characters and press ENTER The display will read Password YES NO ee Note Refer to page 24 for instructions on entering alphanumeric characters Select YES if the protocol is to be password protected The display will read Enter password kkkkkkkk Note If NO is selected the Select plate prompt Step 7 will be displayed Enter the password up to eight numeric characters and press ENTER The display will read Confirm password kkkkkkkk Opsys MW User s Guide Creating a New Wash Protocol 7 Re enter the password and press ENTER The display will read Select plate 2 DYNEX_1 ee Note The plate name that is shown is an example The actual name will be the plate name that is entered for that plate type during system configuration see page 30 8 Use the up and down scroll keys to select the plate type or enter the number using the keypad to be used and press ENTER The 9 display will read Variable strips YES NO er Note Variable Strips is used to run an established protocol on fewer strips than originally specified in the MOVE cycle s of an established protocol If YES is selected the operator will be prompted at run time to specify the number of strips that are to be washed Processing will begin on the first strip selected in the MOVE cycle and will end
53. the value for that operation are displayed In the MOVE example above the Start Strip is 1 the End Strip is 12 and the number of cycles is 1 Opsys MW User s Guide 57 Modifying a Wash Protocol To modify a wash operation 1 Select EDIT while the operation is displayed The first step in the operation is displayed Start strip 1 Cycles Note The actual step that is displayed depends upon the operation being modified 2 Modify the operation using the same procedures as when creating a new wash protocol MOVE 1 10 1 Cycles DELETE EDIT gt er Note In the example above the End Strip was changed from 12 to 10 The actual values that are displayed will depend upon the operation being modified and the new value s entered Note Once the move loop is modified the display will no longer read as shown above Instead it will display the first command in the move loop or End of Move 3 Press ENTER to display the next step on the wash protocol 58 Opsys MW User s Guide Modifying a Wash Protocol To delete a wash operation 1 Select DELETE while the operation is displayed The operation is removed from the wash protocol Note If a MOVE operation is deleted all operations within the MOVE i e Aspirate Dispense Soak and Fill are also removed Opsys MW User s Guide 59 Modifying a Wash Protocol 60 To insert a wash operation 1 Scroll right while the ope
54. ttle 394000400 Sponge Round 60mm Diameter 32000170 Stainless Steel Washer 43000200 Fitting Reducer 3 16 X 3 32 43000390 Hydrophobic Filter 43000491 Quick Connect Fitting Dispense 43000540 Pick Up Filter 816400500 Silicon 5 64 ID x 1 8 OD Dispense 816400600 Tubing Silicon 4 x 7 2 MM Aspirate 816400700 Tubing Silicon 3 x 5 MM Dispense 62503160065 Quick Connect Fitting Aspirate 43000540 Pick Up Filter Major Assemblies 13001541 Bottle Assembly 13001555 Chassis Assembly 13001572 X Z Drive Assembly 43000320 Dispense Pump 582050204 PCB Washer Main Board 582051001 PCB Washer Daughter Board 14000410 Micro Programmed OPSYS MW Main PCB 14000420 PLD Programmed OPSYS MW Main PCB 14000430 PLD Programmed OPSYS MW Daughter PCB Hardware 43000530 Pump Vacuum 12 VDC 528300700 Stepper Motor 50500140 LPS113 Power Supply 12V 6 7A 564300100 Float Switch 0 5 AMP 82 Opsys MW User s Guide Returning a Washer for Service If the instrument must be returned for service it must be cleaned and decontaminated if it has been in contact with potentially infectious body fluids including human blood pathological samples or toxic or radioactive materials ee Note Refer to page 69 for cleaning and decontamination instructions To return a Washer for service 1 Contact the nearest DYNEX Technologies technical service facility for return authorization 2 Clean and decontaminate the Washer
55. urther that the products have not as determined solely by DYNEX Technologies been subject to misuse or abuse by the Customer or other parties unrelated to DYNEX Technologies DYNEX Technologies makes no warranty expressed or implied as to the fitness of any product for any particular purposes other than those purposes described in the applicable operating and servicing manual nor does DYNEX Technologies make any other warranty whether expressed or implied including merchantability other than those appearing on the face hereof Where DYNEX Technologies guarantees any product whether under this Warranty or as a matter of law and there is a breach of such guarantee the Customer s only and exclusive remedy shall be the replacement or repair of defective parts and materials as described above This shall be the limit of DYNEX Technologies liability Furthermore DYNEX Technologies shall not be liable for incidental or consequential damages Failure of the Customer to notify DYNEX Technologies of a claimed defect by registered mail within thirty days of the discovery thereof shall constitute a waiver of any claim for breach of warranty When a product is required by DYNEX Technologies to be installed by a DYNEX Technologies engineer or technician the period of this Warranty shall begin on the date of such installation provided however that any use of the product prior to such installation shall at sole election of DYNEX Technologies void this
56. w CAUTION Always disconnect the power cable before cleaning the instrument To clean the system 1 Clean external surfaces with a cloth moistened with mild laboratory detergent S Note If needed dilute the laboratory detergent according to the manufacturer s instructions before using To decontaminate the system 1 Wipe the surfaces with a cloth moistened with 10 bleach or 70 alcohol Opsys MW User s Guide 69 Cleaning the Containers Cleaning the Containers Procedures for emptying cleaning and refilling the Wash Container and the Waste Container are described below To empty and clean the waste container 1 Unscrew the cap of the Waste Container Lay the cap and level sensor assembly on a paper towel Discard the waste in accordance with local regulations Rinse the waste container with deionized water Discard the rinse water ee Note If desired the waste container can be disinfected with a 10 v v solution of household bleach in water Place the level sensor and tube back into the Waste Container and screw the cap on To refill the wash container 70 Separate the dispense line quick connector in the cap of the Wash Container Unscrew the cap and lay it and the Pick Up Filter on a clean paper towel Fill the Wash Container with the wash fluid that is being used er Note If the wash solution is being changed discard the contents of the Wash Container and thorough
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