Learning Resources LER2344 microscope
Contents
1. MICROSCOPE SET INSTRUCTIONS Only for use by children over 8 years oid Only for use under the supervision of an adult a Read the instructions before use follow them and keep them for C AUTI 0 N reference Keep smali children and animals away from experiments Store 2 the microscope set out of reach of small children Eye protection for supervising adults is not included ADVICE FOR SUPERVISING ADULTS a Read and follow the instructions the safety rules and the first aid information Keep them for reference b The incorrect use of chemicals can cause injury and damage to health Only carry those preparations which are listed in the instructions c This Microscope set is for use only by children over 8 years old d Because children s abilities vary so much even within age groups supervising adults should exercise discretion as to which preparations are suitable and safe for them The instructions should enable supervisors to assess any preparation to establish its suitability for a particular child e The supervising adult should discuss the warnings and safety information with the children before commencing the preparations Particular attention should be paid to the safe handling of chemicals if used f The area surrounding the preparation of slides should be kept clear of any obstructions and from the storage of food It should be in a good light and close to a water supply A solid table with2. and as beautiful as you can imagine Today from the most elementary study of biology to the highly specialized fields of physiology some form of microscope has to be used for the students to understand better the elaborate complicated forms of either living organisms or static materials which make up our living environment Your microscope will be either a source of fun for a long time hobby or an opening door to advanced knowledge in various fields of science We hope you will enjoy using it Battery installation Unscrew the ballery door cover with a philips screwdriver Insert two 1 5V size AA batteries into the battery compartment Replace the battery cover and screw it back into piace Eyepiece Focusing Knob Body Tube Revolving Turret oo Lens rm Stage Clip Mirror Illuminator Lamp Base Battery Case Condenser Lens s Cap ee 0 0 INSTRUCTIONS FOR USE OF MICROSCOPE PNK PRON m a oO oO P 1 First tilt the arm and adjust the position of the reflector so that the light is fully caught by the mirror 2 When the light is fully reflected by the mirror as can be seen through the eyepiece the microscope will be ready for observation 3 Next put the prepared slide on the stage and fix it in place with the clips 4 Now decide what magnification to use The greater the length of the objective lens the greater the magnification Observation is gene
3. a heat resistant top should be provided g Aseparate tin or bucket should be used for the disposal of solid waste materials Any wasted solution should be poured down a drain but never into a sink IMPORTANT TELEPHONE NUMBERS Poison Center Hospital Fire Department Doctor SAFETY INFORMATION General First Aid Information a In case of eye contact Wash the eye with pleniy of water holding the eye open if necessary Seek immediate medical advice b If swallowed Wash the mouth with water drink some fresh water Do not induce vomiting Seek immediate medical advice c In case of inhalation Move person to fresh air d in case of skin contact and burns Wash affected area with plenty of water for 5 minutes e In case of a cut Wash the cut with antiseptic solution if not available use clean water Then put on a bandage In case of any serious injury you should get first aid treatment and inform a doctor as soon as possible In case of doubt seek medical advise without delay Take the material together with the container with you In case of injury always seek medical advise SAFETY RULES a Do read these instructions before use follow them and keep them for reference b Do keep young children and animals and those who are not wearing eye protection away from the experimental area c Do always wear eye protection d Do store microscope sets out of reach of young chi
4. ldren e Do clean all equipment after use f Do wash hands after carrying out preparations g Do not use any equipment which has not been supplied with the set h Do not eat drink or smoke in the experimental area i Do not allow chemicals to come into contact with the eyes or mouth j Do not put foodstuffs in used container Dispose of immediately CAUTION FOR HANDLING 1 The vital part of the microscope is the fens Therefore sufficient care must be taken in handling the lens If the lens gets dirty or dusty wipe the lens surface with a clean lens tissue or soft cotton cloth Do not rub the lens with a finger or dirty cloth etc 2 After the microscope set is used it should be covered with a cloth and be put back into the box for screening from dust 3 Microscope should be stored in a moisture free place Moisture buildup on the light will cause a reduction in light intensity 4 When a microscope is not used for a long period of time remove the light source batteries INTRODUCTION TO A MICROSCOPIC WORLD In this world there is an abundance of living things Some are large enough to be seen with our own eyes but a lot of others are so minute that millions can be squeezed on the head of a pin Of course these tiny organisms can only be seen through a microscope The microscope was invented many years ago Since then it has opened a wider field of research involving many things as fascinating
5. rally made starting at a low setting 5 In order to change the magnification turn the revolving turret until you feel a click 6 Using the focusing knob lower the lens as close as possible to the prepared slide without actually making contact Then while looking through the eyepiece turn the knob in the opposite direction until the image comes into focus 7 When the room is dark with low intensity of natural or lamp light or when the sight is not clear at a high magnification turn the reflector Then the light source lights automatically to enable observation 8 The light source lamp is used with 2 AA batteries put in the back of base HOW TO USE AS A PROJECTION DEVICE Remove the eyepiece and tilt the microscope so that the body tube is pointing at a white surface not more than one meter away For best results view in a darkened room HOW TO MAKE A PREPARED SLIDE If the given sample is not thin and transparent it cannot be observed by the microscope as the light from the reflector or the light source does not pass through it Fibres pollen wool or salt can be observed easily and cover glass is optional Clear samples are stained first with a drop or two drops of methylene blue Eosin or other dyeing solutions available on the market Note These are dyeing solutions and therefore could cause staining of clothing carpets and fabrics Special care should be taken when handling these solu
6. tions 1 Temporary mount Wipe the slide and cover glass clean Thin sample with a razor biade or the micro slicer Note This process should be under an adult s supervision The blade is very sharp and has to be handled with extreme caution Pick up thinned sample with tweezers and put it on the centre part of the glass slide Put one drop of water on the sample with a dissecting needle or if the sample is clear use one drop of the above mentioned dyeing solutions Note The needle has a sharp point so handle with extreme caution and then gently put on cover glass Avoid to trap any air bubbles Remove any excess water or dyeing solution with blotting paper Now it is ready for observation Remember to wash your hans after doing the preparation and remember to dispose the dyeing solutions 2 Permanent mount Wipe the slide and cover glass clean as above Temporary mount Proceed as above However before covering the slide with the cover glass add a few drops of gum media not included in the set available from store or Canada balsam solution or transparent adhesive glue with a dissecting needle to the slide Push down on the cover glass with tweezers to fix it in place and leave to dry for about a day X P38 MS006 81001001
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