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Nikon DS-2MBWC Digital Camera User Manual

1. Explorer Bar a Address Filmstrip Thumbnails Tiles Icons List Details Arrange Icons by Choose Details 0 To Refresh Make anew folder 2 Right click any image file icon of interest and select Properties from the ensuing menu The updated date and time will be displayed amp DS LIIMG File Edit View Favorites Tools Help af pack ki k B JO search ar Folders a Preview Edit Print Refresh Thumbnail iJ View as a slide show Order prints online by Print this picture Rotate Clockwise EN Set as desktop background 1 Copy Eo cE Rotate Counter Clockwise Set as Desktop Background Open with File and Folder Tasks Send To uk Copy imi Rename this File iy Moye this File fe Copy this File Ee Publish this File to the Web A E mail this File 4 Delete this File Create Shortcut Delete Rename Properties 129 U S B VI Basic Operations 5 Using the DS L1 when Connected to a PC by a USB Cable 5 4 5 Removing the DS L1 from the PC Because the DS L1 is connected via a USB interface it can be removed from the PC even while the PC remains powered on However to prevent data from being corrupted while removing the DS L1 during a write operation follow the procedure described below 1 Eject the media from the media drive before removing the DS L1 2 Click the Safe to Remove Hardware icon on the task ba
2. x The current stage position is displayed on the Microscope Status Display Microscopy Setup Menu MIC Info menu To replace specimens or to perform similar tasks select the return to origin icon 0 0 to reposition the stage at the origin Select the icon ool once again to return the stage to its original position If you have difficulty with fine stage adjustments and long exposure times use the CAM menu to adjust the exposure time For more information refer to the explanation column of the CAM menu lt Using Rapid Transfer Mode gt Use this function to move the stage rapidly Perform calibration before using this function For instructions on calibration refer to Performing Calibration and Saving Values 140 VI Basic Operations MICROSCOPE 6 Using the Microscope Control 1 Select the rapid transfer mode icon Et The appearance of the cursor will change to indicate that the DS L1 is set to rapid transfer mode Ki x 2 Left click anywhere on the screen The stage will move to that position with its center aligned with the point just clicked 3 Repeat Step 2 to reposition the stage quickly 4 Right click anywhere on the screen to exit rapid transfer mode Objective lt Changes objectives gt oP On 2 14 1s Compatible only with the Nikon 90i incorporating a motorized nosepiece 1 In the nosepiece column oP select the nosepiece number corresponding to your desired objective The objective is
3. m If you draw a new circle in the middle of or Redo recent measurement results cannot be canceled To save a circle and its measurement results to the overlay of the image press to fix after the measurement Press to return to the menu in which you previously were Only measurement results angles confirmed with will be saved Once the content is fixed as valid you cannot erase it unless you erase the entire overlay NOTE i The display information is interlocked with the menu size Small characters are displayed when you are showing a small menu and large characters are displayed when you are showing a large menu T Normally values with these functions are labeled with the units nm um mm or cm However if calibration values are selected after inch mil conversion they are labeled mil and an E is indicated representing inch conversion T If the microscope connected to your system is the Nikon 80i 90i microscope with motorized or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through s7 Calibration values are automatically changed when you change the objective 77 TOO L VI Basic Operations 3 Measuring Two point Distance or Entering Information TOOL Menu Adjusting the Subject Position and Comparing Sizes 3 5 1 Using Crosshairs to Adjust the Subject Posit
4. D Image save button Lev Exposure level indicator 1 3Mp Display mode for 5M cameras Display mode for 2M cameras PROG Exposure mode 1 250s Shutter speed C_gn Camera sensitivity camera gain io Photometry area p Photometry mode ae CE GAIN Y10O fR100 5100 CHRM 00 HIE SFT 00 CMP Exposure compensation White balance AE lock Gain Image level adjust Color balance adjust CHRM 00 Color density HUE 00 Hue adjust SFT 00 Black level adjust TON1 TON7 Contrast LL SD OFF SD US2 Shading correction ia pi BF Anian NR ON NR OFF Noise reduction SHPO SHP5 Sharpness COLOR B amp w NEGA Effect settings Set value clear button GTA Focus IMMI Focus Focus indicator Info box Indicates information a Custom mode call Scene mode call button 46 VI Basic Operations CAM RA 2 Photographing Images Eat Bo 54 Boo is Cca ofA CHP GAIN Pini Monochrome cameras No color settings are displayed Photographing procedure Follow the procedure described below for general photography By referring to the procedure below find a method that is most suitable for the purpose of observation or the intended subject as you photograph an image 1 Choose display mode 2 Choose exposure mode 3 Choose photometry mode 4 Choose tone 5 Adju
5. Symptom Corrective action Unable to control e Use only the Nikon 80i 90i LV150A Microscope This function cannot microscope functions be used with a microscope from a different company or other Nikon Or incorrect control microscope models results e Turn on the power switch for the microscope e Wait until the microscope is ready for operation It takes about 30 seconds for the microscope to be ready e Connect the USB cable securely between the DS L1 to the digital imaging head on the 80i or to the 90i microscope e Confirm that only one hub stage exists between the PC s USB port and the DS U1 We strongly recommend against using more than one hub e Use the USB cable supplied with the microscope to connect the microscope to the DS L1 e Check whether the Nikon 80i or 90i microscope indicates an abnormal state by buzzer or LED display In the event of any abnormality on the microscope consult the instruction manual for your microscope Unable to display e Make sure the microscope initial setting has been performed microscope status e Change the registration if the objective or filter has been changed Or incorrect status information is displayed 8 Before Contacting Us Please have the following information ready before contacting us e Conditions under which the problem occurs When does it occur What signs indicate the problem How often does it occur e Firmware Version This number can be accessed from SETU
6. using a motorized nosepiece or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through s7 Calibration values are automatically changed when you change the objective 81 3 5 4 VI Basic Operations 3 Measuring Two point Distance or Entering Information TOOL Menu Creating a Translucent Image and Comparing It to a Live Image You can create a translucent image from the displayed image on the screen and view it while being laid on top of the live image for comparison This function may be used to create a translucent scale or inspect registration of shapes The range over which you can create such an image is half the screen 1 2 3 4 5 Press Superimposition Create The tool bar shown below will be displayed with a yellow border displayed on the screen Move the mouse pointer into the area and click it while you drag in order to specify the range in which you want to create a superimposed image Press Create and the system will start creating a superimposed image automatically This will take some time Depending on the subject the image thus created may not have sufficient contrast for easy recognition In such a case use to temporarily erase the image and Lev to change the creation level before using to re create an image If the desired contrast is obt
7. 2 Scale superimposition Cross Create f Disp 66 Changes color or shape Changes display mode and saves settings Superimposition Create Display Information display VI Basic Operations TOO L 3 Measuring Two point Distance or Entering Information TOOL Menu 32 What is an Overlay An overlay is something like a transparent sheet that is placed on top of a photographed image Text count markers pen drawings measurement lines or superimposed images can be written on the overlay The contents written to the overlay can be saved or printed along with the image Basic method of overlay operation All pen drawings text input etc for an image are written onto a single overlay sheet Follow the sequence shown below to create an overlay Choose the function l Draw or enter text l gt To redo clear before redrawing Paste While you are drawing the overlay is simply held in temporary storage so its layout will not be fixed as valid data unless you paste it to the image If you click i to return without pasting the content you ve drawn will be lost Once the overlay is fixed you can choose to display or hide it by using Overlay ON OFF Displaying or hiding the overlay Overlay ON displays the overlay on top of the screen Overlay OFF hides the overlay from the screen Even while Overlay OFF if you press any icon to draw on the overlay e g or Text the overlay will autom
8. 6 Press to return to the menu in which you previously were Only measurement lines confirmed with will be saved Once the content is fixed as valid you cannot erase it unless you erase the entire overlay NOTE i The display information is interlocked with the menu size Small characters are displayed when you are showing a small menu and large characters are displayed when you are showing a large menu Normally values with these functions are labeled with the units nm um mm or cm However if calibration values are selected after inch mil conversion they are labeled mil and an E is indicated representing inch conversion i If the microscope connected to your system is the Nikon 80i 90i microscope with motorized or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through s7 Calibration values are automatically changed when you change the objective 75 TOO L VI Basic Operations 3 Measuring Two point Distance or Entering Information TOOL Menu 3 4 5 Measuring angles You can measure an angle between two lines on the screen and save the measurement line lines and angle for this measurement as part of the overlay for the image 1 Press to display the following toolbar Select the wh icon ZELKO Undo Fe Reso 7 Clear g Paste 2 Press a color icon red blue gr
9. IMG1280 bmp IMG1600 bmp For detailed information on file settings refer to 1 4 2 Image File Settings in Chapter VI NOTE T To capture files at maximum resolution 2560 1920 for 5M cameras or 1600 1200 for 2M cameras you must set the correct parameters for size and the correct display mode The display mode also needs to be set to maximum resolution 5Mi for 5M cameras or 2Mfull for 2M cameras Setting the display mode to a lower resolution will result in image captures at the lower resolution Do not try to capture image files while the display mode is set to C Scan Regardless of the file setting this display mode will result in an image constituting only a portion cropped from the center of the original image The image files stored in the DS L1IMG folder are updated once every 5 seconds Be aware that if files are read out at intervals shorter than 5 seconds you will not always get the latest image To confirm the time of day at which image files have been updated follow the procedure described below 126 5 VI Basic Operations U S B 5 Using the DS L1 when Connected to a PC by a USB Cable Confirming the updated date time of image files 1 Open My Computer and display image file icons Then select View Refresh to update the image file icons displayed DS L1IMG Tools Help Eis Search ian Folders Fee File Edit Pa Favorites e Back E Toolbars Status Bar
10. If the source is set to s2M the CCD mode switches to match the shutter speed resulting in delays during image acquisition Progressive display modes eliminate color shifts even with moving subjects An image area of 800 560 pixels is enlarged for display at the monitor resolution 1280 960 SXGA or 1024 768 XGA The top and bottom 40 pixels of the 1200 vertical pixels are cropped out and therefore not displayed The frame rate is 30 fps This display mode is useful when good performance with moving subjects is required If the source is set to s2M the CCD mode switches to match the shutter speed resulting in delays during image acquisition Progressive display modes eliminates color shifts even with moving subjects An area corresponding to the center of the image 560 pixels out of 1200 vertical pixels is formatted for display at the monitor resolution 1280 960 SXGA or 1024 768 XGA The frame rate is 30 fps This display mode is useful when good performance with moving subjects is required Regardless of the source setting the CCD mode switches to match the shutter speed resulting in delays during image acquisition Progressive display modes eliminates color shifts even with moving subjects 34 VI Basic Operations SETU P 1 Preparations Before Photographing Auto change CCD mode Select Auto change CCD mode for automatic adjustment of the CCD mode to match the camera shutter speed when the source is set t
11. Setting the FTP client function To set the FTP client function of the DS L1 select Network Settings from the SETUP menu to call up the Network Settings screen shown below Power n Settings NetWork Settings File Settings Additional Return to Window NetWork Settings Enable NetWork Read Only FIP WEB disable Write Control BGP C duto get an IP Internal Server 1P address 192 Sublet Mask 255 User ID anonymous max 10 char Pass Word none pies A0 aian Enables disables network Enable FIP Client 1P authority operation ing test Sets the IP address of User ID max 10 char the DS L1 Pass Word max 10 char cate er 192 168 5 L6 L7 L809 MAS Address Enable FTP Client For image files to be sent to the FTP server always select this check box to enable the FTP client function External Server Set the IP address and the gateway address of the server PC Before images can be sent to any FTP server that belongs to a different network group you must enter the gateway settings Server No Five servers can be stored in memory Assign each server a number IP Address lt Input example User ID Enter a user ID that is needed to log in to the server Pass Word Enter a password that is needed to log in to the server as necessary Gate Way lt Input example If any entry in the IP address consists of two digits or one digit add O or OO at the beginning of i
12. The position of the X scale moves as you move the mouse When the X scale position is where you want it release the mouse button to set the position of the X scale The position of the Y scale can be moved in the same way To hide the displayed scales press again NOTE It may not be possible to display XY scales depending on the selected calibration value If XY scales cannot be displayed a message C Scale NG is displayed in the Info area In that case change the calibration unit that has been set for example from 1 mm to 1000 um i Normally values with these functions are labeled with the units nm um mm or cm However if calibration values are selected after inch mil conversion they are labeled mil and an E is indicated representing inch conversion i If you perform an electronic zoom while using this function the screen display will reflect the zoom magnification i If you change the optical zoom of the digital imaging head while using this function with the Nikon 80i 90i microscope connected to your system the screen display will reflect the zoom magnification j If you change the objective while using this function and if the microscope connected to your system is the Nikon 80i 90i microscope using a motorized nosepiece or intelligent nosepiece the screen display will change accordingly If the microscope connected to your system is the Nikon 80i 90i microscope
13. change the drive letter assigned to the media drive or the CCD image drive The following shows how to make this change Select Start Control Panel Administrative Tools Computer Management Disk Management Right click the volume whose drive letter you want to change and select Change the drive letter and path Click Edit and choose one of unassigned drive letters from the select box or enter a drive letter directly from the keyboard and click OK Click OK in the ensuing confirmation dialog box 1260 5 4 3 VI Basic Operations U S B 5 Using the DS L1 when Connected to a PC by a USB Cable Using the Media Drive Sending receiving image files Insert media into the card slot of the DS L1 and display the Explorer screen from the confirmation screen described above You can access media from this screen Or you can double click the media drive removable disk icon located below Devices with Removable Storage on the My Computer screen to access the media The image files on the media drive can be retrieved from the media or transferred from the PC to other media files in the same way as files are normally manipulated on a PC Removing media 1 2 3 4 Check to see that the PC card indicator on the DS L1 is turned off Close the menus displayed on the DS L1 If any image files have been loaded from the media to the PC close all of the open image files on the PC Right click the medi
14. connect an external monitor co e CAMERA E FL J6 Camera connector Connect the DS camera head to this connector Always be sure to turn off the power before connecting or disconnecting the camera head to or from this connector Failure to do so may result in malfunction External interface input Connect the DS Remote Controller DS RC here and you can perform freeze and capture operations from the DS RC A foot switch etc may also be used providing it meets the interface specifications Refer to 3 Connection Methods in Chapter IV for details on the interface specifications External interface output Connect a stroboscope or other external device here This output generates a synchronizing signal when capturing an image enabling the strobe light to be turned on synchronously Refer to 3 Connection Methods in Chapter IV for details on the interface specifications III Names of Parts and Their Functions 2 DS Camera Head DS Camera Head Tripod mount This mount can be used to mount the DS camera head on a tripod Top Rear Bottom C mount cap Protects the C mount from dust C mount CAMERA OUT connector When taking microphotographs mount the C mount adapter here before connecting to the microscope When taking other photographs attach the C mount lens here FREEZE CAPTURE FREEZE button Freezes the current image as a still picture To rel
15. DS L1 2 Turn on the modular microscope then turn on the DS L1 3 To switch to a certain nosepiece open the menu and select the corresponding number LY NCNT fi 10x MODULE NAANA 4 After switching nosepieces confirm that the selected number icon is indicated by a red border and that the nosepiece number and the objective information are displayed NOTE ii Information entered during calibration will be displayed in the information column of the current objective Switching objectives will delete all overlay data with the exception of the XY scale and the scale currently displayed For more information on overlay data refer to the description of the TOOL menu ii Switching objectives will also change the XY scale and the scale currently displayed Lo VI Basic Operations MICROSCOPE 6 Using the Microscope Control 63 Autofocus Precautions ZN CAUTION Before using autofocus check your microscope for the following Disregarding these precautions may result in damage to the objective or sample Precautions for samples 1 The autofocus function may be used with samples mounted on specimen slides with cover glass in the sizes indicated below e Thickness 0 9 mm to 1 7 mm inclusive thickness of slide glass thickness of cut piece not including the cover glass e Slide glass Complies with JIS R3703 or ISO 8037 e Cover glass Complies with JIS R3702 or ISO 8255 JIS R3702 Thickness
16. DS L1 and the equipment to be connected before making any connections In addition be sure to consult your network administrator Make the connections as shown below We recommend using cables and hubs designed for LOOBASE TX use Connecting to a LAN Left side of the DS L1 Network Third party 10 100BASE TX A category 5 cable straight A 4 To Ethernet connector Third party 10 100BASE TX category 5 cable straight 14 IV Connecting and Installing the Equipment 3 Connection Methods Connecting to a PC without going through a LAN Left side of the DS L1 gt Third party 10 100BASE TX category 5 cable crossed To Ethernet connector 7 Connecting External I O Devices Be sure to turn off the power to the DS L1 and any equipment to be connected before making connections When an external device is connected to the EXTERNAL IN connector on the DS L1 the image can be frozen or captured by trigger input from the external device Furthermore if an external device is plugged into the EXTERNAL OUT connector a strobe synchronizing output can be obtained when capturing an image If you are preparing your own external device use a device that satisfies the specifications shown below Connector 3 5 mm diameter stereo pin jack EXTERNAL IN connector Connector 2 5 mm diameter stereo pin jack EXTERNAL OUT connector i
17. DS camera head on a microscope 1 Remove the C mount cap from the C mount AOS on the DS camera head To CAMERA connector 2 Make sure there is no dust on the C mount on the DS L1 adapter If there is use an air blower or similar means to remove it DS camera head C mount 3 Screw the C mount adapter into the C mount C mount adapter on the DS camera head Screw it in as far as it will go 4 Insert the C mount adapter into the vertical Vertical tube of microscope tube of the microscope Use the clamping screw on the vertical tube to secure the C mount adapter in place You can also attach a relay lens We recommend the x0 7 relay lens for the DS 5M and DS 5Mc We recommend the x0 55 relay lens for the DS 2Mv DS 2MBW and DS 2MBWc When you have mounted the DS camera head ona microscope be sure to read Chapter V Microscope Adjustment as well Installing a third party C mount lens on the DS camera head Select a mountable lens see Chapter II Peripheral Equipment then screw it into the C mount Note that the image quality produced by this product is tuned for microphotography The tripod mount on the DS camera head can also be used to mount the DS camera head on a tripod 2 Connecting an External Display Be sure to turn off the power for the DS L1 and the display before making any connections Connect one end of the D sub 15 pin connecting cable supplied with the unit to the RGB connector on the DS L1 and th
18. DS1 FTPIBOX When accounts are added separate directories are created for each user so that even if there are multiple users DS L1s connected to the network image files are saved in each separate directory Access rights for the created folder are set so that only the user who created it can access it with both read and write privileges other users may access the folder only to read files Do not change the access rights for the created folder Doing so may make it impossible to transfer images to the folder or result in other users inadvertently deleting images 108 VI Basic Operations N ETWO RK 4 Using the DS L1 via a Network Other settings Network settings and confirmation Network settings and confirmation can also be accomplished by selecting Macintosh HD Applications Utilities Network Utility e The Info tab Shows the IP address and other information e The Ping tab Sends a ping command To use a function that is equivalent to the command prompt in Windows select Macintosh HD Applications Utilities Terminal to display the Terminal screen 1097 N ETWO RK VI Basic Operations 4 Using the DS L1 via a Network 4 6 2 Setting External Servers of the DS L1 Network Settings Consult the network administrator in your organization for details on the FTP server If you are going to connect the DS L1 to a LAN you need to enable it for network operation and set its own address
19. My Computer screen to access the media The image files on the media drive can be retrieved from the media or transferred from the PC to other media files in the same way as files are normally manipulated on a PC Removing media When the DS L1 is connected to a PC follow the procedure described below to remove the media from the media drive 1 Check to see that the PC card indicator on the DS L1 is turned off Close the menus displayed on the DS L1 2 If any image files have been loaded from the media into the PC close all open image files on the PC 3 Right click the media drive removable disk icon on the My Computer screen and select Eject My Computer gt File Edit View Favorites Tools Help da Back S Search Lt Folders EH Ss Gs x wy Ei Address My Computer 15 x 344 Floppy 4 Li Local Disk 01 fie Local Disk Ds My Computer Local Disk E Local Disk Fs Removable Disk 1 2 Compact Disc G Removable Disk J Removable Disk HH Open Capacity 60 8 MB SA User c ee g 4 EP Psgdat gt h Used 22 6 ME ay Control earch C Free 38 2 MB Sharing Cal Winzip Format Create Shortcut Rename Properties Ejects the removable disk From the drive 4 Remove the media manually from the DS L1 e121 U S B VI Basic Operations 5 Using the DS L1 when Connected to a PC by a USB Cable 5 3 4 Using the CCD Image Drive Receiving i
20. OFF SD10 to SD 50 SD US1 SD US2 Correct unevenness in shades on the screen To correct for a reduction in luminous energy from the middle part toward the periphery of the screen use 10 to 50 correction data Watch the screen while you select the correction data When you are correcting nonsymmetrical unevenness etc follow the procedure described below to save the contents of your corrections The saved data can be called onto the screen later for when you are correcting other images 1 2 3 4 5 Be sure to remove dust from the optical path if it is visible on the screen Set the screen to become evenly white overall except for the subject Adjust the amount of incident light so that while using AE or MANU exposure the shutter speed is in the range of about 1 7 5 to 1 250 seconds Press to enter the Registration menu Press SD Set If shading correction has been set normally you will see displayed on the screen Select the number in which you want to save your settings and press to save Note that shading can be corrected up to about 50 If the shading correctable range is exceeded an SD Data Error message will be displayed To correct shading with the saved data choose the shading correction icon SD US1 or SD US2 from the Advn menu 7 Confirming exposure information There are two methods to confirm the exposure information After confirmation adjust the exposure as necessar
21. Open Opens or closes the shutter Es Filter eave Shows filter cube name Oe jE Selects the filter cube to use Turns the lamp on or off FEH Sets a preset voltage 9 V Info box for information display Shows the operating status or detail items of the icon whose operation is selected 138 VI Basic Operations MICROSCOPE 6 Using the Microscope Control mE Focus Adjusts focus Focus ESLAPE Compatible only with the Nikon 90i microscope 1 Set the coarse fine select icon 1 1 to coarse motion mode The arrow icon changes shape for coarse motion and fine motion modes for coarse motion mode for fine motion mode 2 Use the Up icon andthe Down icon to adjust the focus position 3 Finally to fine adjust set the coarse fine select icon to fine motion mode and use the Up icon and the Down icon to adjust the focus position NOTE i Information on the height of the current Z position is displayed on the microscope status display microscopy setup menu MIC Info menu To replace specimens for example select the escape icon BIE The stage will descend to a height at which you can replace specimens Select the escape icon BAPE again after replacing specimens to return the stage to its original position a If the exposure time is long and you have difficulty fine adjusting the focus position use the CAM menu to adjust the exposure time For more informatio
22. Pin 3 GND Pin 2 Pin 1 EXTERNAL IN Functions Pin 1 FREEZE Pin 2 CAPTURE You will not be able to capture desired images at precise split second moments Use of this input is not suitable for photographing fast moving objects using a sensor 3 3kQ Pin 1 or 2 Activated when the switch is ON closed DS L1 side Required pulse width 10 ms or more 415 lt IV Connecting and Installing the Equipment 3 Connection Methods EXTERNAL OUT Functions Extended external output pins 1 and 2 both for strobe synchronizing output This output is used mainly for synchronized firing of a stroboscope when capturing an image with the DS L1 Electrical characteristics of strobe synchronizing output are open collector output maximum input current 10 mA withstand voltage 24 V and internal resistance 100 Q The output pulse width is 67 ms This output can be connected to external devices that satisfy the following electrical characteristics See the diagram below e Strobe synchronizing output is tied to a power supply line of 24 V or less through a pullup resistor R on the external device side e Inputs on the DS L1 and external user device sides have the same GND potential or are left floating E lt 24V 1000 R lt E 10 kQ T Pins 1 and 2 T j p ON time level 100xE R 100 V OFF time level E V O Pin 3 GND v v 7 GND DS L1 side External device side ZN CAUTION When plugging a stroboscope or other e
23. Quality If you select JPEG for the file format select a compression rate picture quality here 34 lt SETU P VI Basic Operations 1 Preparations Before Photographing Fine Provides high picture quality but produces large images due to a low compression rate Draft Provides low picture quality but small file sizes due to high compression rates NOTE You can also select the file format when saving an image from the REC menu The following table gives the resulting file sizes for various image file settings Since actual file sizes of JPEG images can vary widely from image to image the JPEG values given here are intended to serve only as a guide File Type JPEG JPEG JPEG JPEG Fine Normal Basic Draft 5M cameras 2560 1920 14MB 4MB 1 8MB 1 2MB 900KB 1280 960 3 6MB 1 0MB 460KB 310KB 230KB 640 480 900KB 260KB 120KB 80KB 60KB 2M cameras 1600 1200 5 6MB 1 6MB 720KB 480KB 360KB 800 600 1 4MB 400KB 180KB 120KB 90KB 400 300 350KB 100KB 50KB 30KB 20KB Separate R G and B channel data is stored Overlay composites can be created by incorporating colored drawings with a pen or other tool When you save overlays with the TOOL menu keep in mind that it may be difficult to read text information if the file is too small or features high JPEG compression We recommend saving files that include text information in BMP format at 1280 960 resolution or higher Source Setting Guidelines Photography mode The source setting re
24. Saving an Image to a CF Card REC Menu Select the REC tag at the top of the CAM menu screen The REC menu will be displayed In the REC menu you can specify a directory name and a file name before saving an image You also can choose interval photography which lets you shoot an image successively at given intervals In addition to the REC menu you can use the panel switch on the main unit or the CAPTURE button on the DS Remote Controller DS RC to save images to a CF card Before you can do this you must first select Save to Card for Capture Function in Additional Settings of the SETUP menu NOTE Refer to 2 1 1 Simple Photography Easy Menu in Chapter VI for details on how to use the CAPTURE button to save images The following describes how to save images from the REC menu Configuration of the REC menu Select the CF FTP check box and an image will be saved to both Press CF to save an image to a CF card Click the directory name to the right of to specify a CARD ie directory Fia Dir FTOS0904 Press to save an image to an FTP server 1 010 001 anonymous File 0419mmes JPG Specify a file name 1shot Only one image is saved Continue Interval photography photographed at given intervals JAttach Log File Source sok 1280 960 Norma NOTE Refer to 4 6 Saving Images to an FTP Server PC from the DS L1 in Chapter VI for details on how to save images to an FTP server from the REC me
25. VI Basic Operations 3 Measuring Two point Distance or Entering Information TOOL Menu Entering Textual Comments lott No 1123 The text function allows you to enter a textual comment in the image 1 2 3 4 5 6 NOTE Although text can be entered from a keyboard you need a mouse to perform other operations Press Text The tool bar shown below will be displayed K2 OOOO O BOR S Position E Paste Press the red blue green black white or yellow color icon to select the text color Click the input window with the mouse to display a keyboard on the screen You will see the words Input Mode displayed on the upper left part of the menu Click the character on the keyboard that you want to enter When you have finished press to confirm your entry If you missed entering a character use to erase all characters or to erase one character To enter characters from your keyboard move the cursor to the text input window and press the Enter key Use the SHIFT Backspace keys on the keyboard to erase all characters or the Backspace key to erase one character Type your comment on the keyboard and hit the Enter key to confirm the content of your comment The Input Mode message disappears Determine the position in which you want the text to be embedded Use to display an embedding area Place the mouse pointer within the area and drag the embedding area to the desired position Once you
26. ace asic vciadaeety aaaaneaals Gard ob pene sey cae one Soaaeremaaed ine 84 Auk TY piGal MEENOG OT WSC ria E EA E EAA N AA E yak tute eecaalnes 84 42 NCEWOFK SENOS irnir aea tGrannuatonese atau cuneate ortueiead anna tet seas penton ecnnuaes 86 4 2 1 Items Needing Confirmation cc cece cece eee cece cece cece cece a a a E aa 86 4 2 2 Setting Essential Items Network SettingS ccc cece eee eeeeeeeeeeeeeeeeeeeaaas 87 4 3 Operating the DS L1 from a WEB BroOwWSel cccccceeeeeeeeee eee eeeeeeeaeneeeeeeeeeas 90 4 3 1 Setting the WEB BrOWSE vccscvsc ecececet ntsc ai nad eneceasd ute E REEE 90 4 3 1 1 When Using Internet Explorer cc cccccccsseeeseeeeeeeeeeeeeseeeeseenaannteeeeegs 90 4 3 1 2 When Using NetSCa DG iiscniccnaccadatesnordataidind stat nos aaue dinates need stuadiad ates wees 92 4522 USING WEB SCreGnS ns2 c05h nanbate nana E EE eh ald E els onan 94 4 4 Using FTP Commands to Operate the DS L1 cece ceccce cece eee eeeeeeeeeee eens 101 4 4 1 Using FTP Commands to Get DS L1 Image Files from a PC 05 101 4 5 Using Telnet Commands to Operate on the DS L1 cece eeee ee ee eens 102 4 6 Saving Images to an FTP Server PC from the DS L1 ccceeeeeeeeeeeeeeeeeeees 103 4 6 1 Setting the FTP Server Functions of the PC ccccceeeeeeeeseeeseeenennnenes 103 4 6 1 1 When Using Windows NT Workstation Ver 4 0 ccccccceeeeeeeeeneee eens 103 4 6 1 2 W
27. anonymous FTP password Proxies Cookies SmartUpdat F ci tees Accept all cookies Accept only cookies that get sent back to the originating server C Disable cookies M Warn me before accepting a cookie coneat toe Left click here OD VI Basic Operations N ETWO RK 4 Using the DS L1 via a Network 5 Select the check box labeled Manual Proxy Configuration and then left click on View Preferences Category Appearance P ies Be Configure proxies to access the Internet Fonts mmu Colors a Navigator A network proxy is used to provide additional security between your Languages computer and the Internet usually along with a firewall and or to Applications increase performance between networks by reducing redundant traffic Smart Browsing via caching Mail amp Newsgroups C Direct connection to the Internet H Roaming Access H Composer Manual proxy configuration View Left cl ick he re H Offline C Automatic proxy configuration Ee Advanced Configuration location URD Reload SmarUpdate Left click here 6 In the Exceptions column write the ID address of the DS L1 Refer to 4 2 2 Setting Essential Items in Chapter VI for details on the IP address of the DS L1 e Enter a period after every three digits of the IP address e If the first digits of the IP address are 00 or 0 do not enter the zeros Manual Proxy Configurat
28. at the same time media is inserted into the media drive For the CCD image drive because it is labeled NIKONIMG this screen is displayed as shown below The drive name differs with each PC environment In this confirmation screen for either the CCD image drive or the media drive select the Open Folder to View Files Using Explorer check box and the Always Do the Selected Action check box Then click OK button NIKONIMG I Windows can perform the same action each time you insert oa disk or connect a device with this kind of file Pictures What do vou want Windows to do ee using Photo Printing wizard View a slideshow of the images using Windows Picture and Fas Viewer es Copy pictures to a folder on my computer Using Scanner and Camera Wizard Open folder to view files using Windows Explorer Take no action mm When you click OK button the Explorer screen opens showing the content of each drive NOTE 1 2 3 4 In the following cases there might be a conflict between another connected device and the drive letter When media is inserted correctly into position the message Please insert media is displayed when you click the icon for the media drive A similar message is displayed when you click the icon for the CCD image drive When you connect the DS L1 other USB devices already connected to the PC will immediately become unusable In such a case
29. check box the direction buttons in 7 and the sample image in 8 are displayed with the preview screen displayed in a 4 fold enlarged view The gray region in the sample image white rectangle shows an enlarged view of what is currently being displayed If you want to change the position of the sample image currently being displayed in an enlarged view click directly in the new position that you want to be enlarged or click one of the four up down left right direction buttons If you deselect the E Zoom check box the E Zoom function is turned off and the direction buttons and the sample image disappear with the preview screen displayed in normal size If high picture quality 1280 x 960 is selected for the picture quality of the preview screen a middle quality 640 x 480 image is displayed in an enlarged view 9 Location The DS L1 installation information etc that has been set by Telnet commands is displayed here Settings by Telnet commands Connect from an external PC to the DS L1 by Telnet Refer to 4 5 Using Telnet Commands to Operate on the DS L1 in Chapter VI Execute the command w_LCA_ string 32 characters and information to Location can be written The string can be entered using up to 32 ASCII characters which when displayed appear in uppercase letters The information is displayed on the screen when you restart the DS L1 by turning it off briefly and then on again 10 Preview screen Use this scree
30. condenser module you want to use ri Ee Ea Ea 7 Diascopic illumination aperture diaphragm i Adjusts the diaphragm co Diascopic illumination field diaphragm Adjusts the diaphragm aaa Episcopic illumination field diaphragm Adjusts the diaphragm al ND filter Adjusts the transmission factor lt B gt Analyzer Eai Info box information display indicates operating status or detailed items for the selected icon 145 M OOOO VI Basic Operations 6 Using the Microscope Control Condenser lt Changes condenser module gt 1 2 g KN EA EN i EA fe A Compatible only with the Nikon 90i microscope In the condenser column Le select a condenser number to which your desired condenser is registered The condenser will change to the one you select After switching condensers confirm that the selected number icon is marked with a red border and that the condenser number and name are displayed NOTE Before using this function you must set up the condenser information from a PC with the setup software For more information refer to the instruction manual for your microscope i Right click on an icon representing a condenser number and the names of all registered condensers will be displayed You also can select the desired condenser from within this display to change condensers Diascopic illumination aperture diaphragm lt Adjusts the diascopic illumination aperture
31. diaphragm gt Compatible only with the Nikon 90i microscope 1 2 In the diascopic illumination aperture diaphragm column Es select the j icon to open the diaphragm in increments of 0 1 Or select the icon to close the diaphragm in decrements of 0 1 Briefly depress the mouse button to adjust the diaphragm in three increment steps NOTE The level indicator shows the current aperture of the diaphragm in increments of 1 4 in the range 2 0 to 30 5 el Diascopic illumination field diaphragm lt Adjusts the diascopic illumination field diaphragm gt Compatible only with the Nikon 90i microscope 1 2 In the diascopic illumination field diaphragm column co select the j icon to open the diaphragm in increments of 0 1 Or select the icon to close the diaphragm in decrements of 0 1 Briefly depress the mouse button to adjust the diaphragm in three increment steps 146 VI Basic Operations MICROSCOPE 6 Using the Microscope Control NOTE The level indicator shows the current aperture of the diaphragm in increments of 2 0 in the range 0 8 to 30 6 o Episcopic illumination field diaphragm lt Adjusts the episcopic illumination field diaphragm gt Compatible only with microscopes incorporating the DIH E 1 In the episcopic illumination field diaphragm column lean select the icon to open the diaphragm in increments of 0 1 Or select the icon to close the diaphragm in decrements of 0 1 2
32. essHese255 We recommend selecting Auto for the file name in File Settings so that files will be named after the photographed date and time 114 VI Basic Operations U S B 5 Using the DS L1 when Connected to a PC by a USB Cable 5 O Using the DS L1 when Connected to a PC by a USB Cable 54 Recommended Operating Environment When the DS L1 is connected to your PC by a USB cable you can read or write to the recording media inserted in the DS L1 or capture the live image being shot by the CCD camera onto the PC Supported PCs The DS L1 can only be connected to a Windows based However this is limited to PCs that have a USB interface board installed Supported OSs e Windows 2000 Service Pack 3 SP3 e Windows XP If you are using Windows 2000 be sure to update its version to SP3 Otherwise data may not be read or written to correctly About the recording media used by the DS L1 e Usable media Compact flash card Type I and II microdrive e Media formatting When formatting media in your PC make sure it is formatted in FAT12 16 Sometimes these are expressed simply as FAT or in FAT32 Note that depending on the OS used you may not be able to select a format The DS L1 has a function for formatting media The media preformatted in NTFS and so forth that is formats not supported by the DS L1 are formatted in FAT16 For details on how to use the formatting function refer to 2 2 2 Reproducing Images f
33. gt 70dB A oder weniger gem EN ISO 7779 168 IX Technical Specifications 2 DS Camera Head 2 o DS Camera Head DS 2Mv DS 2MBW DS 2MBWc DS 5M DS 5Mc Image pickup device 1 1 8 inch square 2 110 000 pixel interline CCD 2 3 inch square 5 240 000 pixel interline CCD Color black and white Black and white Color Infrared blocking filter Built in None Built in Frame rate 1 15 0 fps 2M Full 3 8 fps 5Mi 20 0 fps 800 6 6 8 fps 1 3Mi 30 0 fps 800 5 7 5 fps 1 3p 30 0 fps C Scan 15 0 fps C Scan Number of recording pixels 1 600 x 1 200 2 560 x 1 920 800 x 600 1 280 x 960 400 x 300 640 x 480 Quantization 12 bits Sensitivity Equivalent to Equivalent to ISO 350 Equivalent to ISO 64 ISO 100 with settings of Tone 3 and x1 camera sensitivity Lens mount C mount Usable lens Protruding no more than 10 mm from the mount surface into the camera Image circle 8 9 mm in diameter Image circle 11 mm in diameter External dimensions 91 W x 76 D x 41 H mm Weight 260 g 290g 260 g 290g Fixing tripod mount Tripod screw for camera Body color White White Black White Black with logo with logo with logo CCD cooling ambient ambient _ o temperature 20 _ temperature 20 C C Peltier cooling Peltier cooling 1 Indications appearing in the Advn menu are shown in parentheses EEE Ds Remote Controller DS RC External dimensions 56 W x 68 D x 21 H mm Weight 110g 169 IX Technical
34. have determined the position press to fix the position in which to insert the comment Press to return to the previous menu NOTE The display information is interlocked with the menu size Small characters are displayed when you are showing a small menu and large characters are displayed when you are showing a large menu 70 VI Basic Operations TOO L 3 Measuring Two point Distance or Entering Information TOOL Menu 3A Measuring on the Screen Once you calibrate the reference length you will have a simple means of measuring distances You can save seven calibration values for each objective or measurement condition and reuse saved values by calling them up the next time you perform a measurement 3 4 1 Performing Calibration and Saving Values Before measuring the length of anything you must first calibrate the reference length Be aware that you cannot perform measurement if you skip the calibration process _ There are two ways to calibrate You can use a subject of known length Il or you can use the magnification of the objective and relay lenses mit You can switch between these two methods by right clicking the icon The icon will change accordingly Icon when using a subject of known length Icon when using the magnification of the objective and relay lenses lt When using a subject of known length gt NOTE If the microscope connected to your system is the Nikon 80i 90i microscope set the m
35. installation and storage of the equipment Be careful to avoid pinching your fingers or hands in the equipment during installation To prevent damage to the equipment avoid subjecting it to physical shock or violent vibrations during installation If you will not be using the equipment for an extended period of time unplug the power cord from the power outlet Store equipment in a location where it will not be subject to vibrations or excessive amounts of dust Avoid storing the equipment in hot humid locations CONTENTS CONTENTS I Berre USE ritarar aa A E E a a 1 1 Typesof DS Camera Headriaracaiine a a A a aa due ew A a 1 2 COMPONENTS aeieea a a a nN NEE R aa A 2 i Penpneral EQUIDEM rrsireririnireriaie n eiee ar anr oeeie 3 I Names of Parts and Their Functions ssssssssssesrensrsnrsnsnrnnnnrnenrrnrrrenerenrrnr 6 t DS Camera Control UIC DS Linra A 6 2 DS Camera Hedd sista panor tage wane a E A EAE TAAR 9 3 DS Remote Controller DS RC Optional 5 cccccsccscccseccsevaescseneucesecapssseusuneeas 9 IV Connecting and Installing the EQuipMent cc cccceee esse cent ee eeeeeeseeeeneeeeenes 10 T CONNECTION DiagiaIMSictickicciaiaieseehest seed ates E Me leieahiaieessudiee sts 10 2 TAS ah Che Dos ts 2324 3 diieuwlay hie anensa A pian iiaewamed A 12 3 GORMECCION MCUINOGS sssst iansasaciannaeesaae E O sade eaeue ear aneasans 13 Y Microscope Adjustment terrieri anapeana 17 VE Basic Operation S eero ae tun enlea
36. new hardware has been detected You do not need to perform any specific operations Ji Found New Hardware Recycle Bin USB20 Devices lows AP Professional valuation copy Build 2505 ee By ale 1 40 PM i Found New Hardware Recycle Bin lows AP Professional USE Mass Storage Device Poria valuation copy Build 2505 When the Safely Remove Hardware icon is displayed in the task bar it means the PC has finished installing the driver aaj ately Remove Hardware f When the DS L1 is connected to a USB 1 1 compliant USB port the message shown below will be displayed in the task tray system tray This is not an error message The DS L1 will operate as a USB 1 1 device AK HI SPEED USB Device Plugged into non HI SPEED USB Hub 4 HI SPEED USB device is plugged into a non HI SPEED USB hub For assistance in solving this problem click this message Recycle Bin lows AP Professional valuation copy Build 2505 ae fim NIKONIMG 13 p gt Bg aos 1 41 PM 1255 USB VI Basic Operations 5 Using the DS L1 when Connected to a PC by a USB Cable 5 4 2 Outline of the Disk Drive When the DS L1 is connected to a PC the disk drive for the CF card etc and the internal image drive of the DS L1 are each recognized as one removable disk by the PC A screen may be displayed prompting for confirmation of how you want the removable disk to be handled by the OS For the media drive this screen is displayed
37. objective Avoid over enlargement The scaling factor should be set in a range of 500 to 1 000 times the numerical aperture of the objective Set the exposure or tone to an appropriate level gt See 2 Photographing Images in Chapter VI Set the microscope illumination at an appropriate level 156 Symptom The color is poor The contrast is low Noise appears at the edges of the menu when shown on an LCD monitor or projector VIII Troubleshooting 2 Image Output Corrective action Set the white balance gt See 2 Photographing Images in Chapter VI Apply a moderate amount of color compensation gt See 2 Photographing Images in Chapter VI Turn off hue adjustment gt See 2 Photographing Images in Chapter VI Set the color temperature of the internal monitor and external monitor to an appropriate level gt See 1 4 4 Adjusting Monitor Output for an External Monitor in Chapter VI Set the color temperature of the illumination to an appropriate level Set the tone appropriately gt See 2 Photographing Images in Chapter VI Avoid adjusting the black level to the positive side of SFT gt See 2 Photographing Images in Chapter VI Adjust the size of the field diaphragm so that it circumscribes the viewfield Adjust the size of the aperture diaphragm to approximately 70 to 80 of the numerical aperture of the objective gt See Chapte
38. right of to specify a FTP 1 010 001 anenymous directory File 0419mmss JPG Specify a file name 1shot Only one image is saved Continue Interval photography photographed at given intervals Attach Log File O 1 28Lbe46U Norma NOTE Refer to 2 2 1 Saving an Image to a CF Card REC Menu in Chapter VI for details on how to set in the REC menu SLs VI Basic Operations 4 Using the DS L1 via a Network Saving an image To save an image to the FTP server from the REC menu follow the procedure described below 1 2 3 Choose 1ishot if you want to save only one sheet image Choose Continue to save multiple sheets of images at given intervals Change the destination or file name to or in which to save as necessary If you want to Save a log enter a comment in LOG Press FTP and the image will be saved to the FTP server If you did not change the destination in step 2 the image will be saved using the same folder name file name file format and compression rate that were set in File Settings Refer to File Settings 1 4 2 Image File Settings in Chapter VI for details on the format of the saved image Also refer to the preceding sections for details about the recommended examples for file names etc Interval photography Interval photographing refers to photographing an image successively at specified time intervals To perform interval photographing follow the proce
39. setting each item press SAVE to save the data that has been set Or press Default to restore data to default values CAM Custom No 1 7 The set values for the subject objective etc can be registered in custom mode Here you can specify the number for the custom mode to be used at startup NOTE The contents of the custom mode can be set from the CAM menu Cam OSD Ind Bio Select the type of scene mode button to be displayed in the CAM Easy menu Cross Line Selects whether to display or hide crosshairs when the power is turned on Refer to 3 5 1 Using Crosshairs to Adjust the Subject Position in Chapter VI Display Custom No 1 7 Seven types of screen display patterns can be registered Here you can enter the number for the desired display pattern At startup the screen is displayed according to the settings of the pattern number you last saved OSD Position U Left U Right Select the position upper left or upper right where you want the menu to be displayed Size Small Big Select the size at which you want the menu to be displayed 36 VI Basic Operations SETU P 1 Preparations Before Photographing SXGA XGA LCD Select a monitor output state from those listed below XGA LCD ON LCD Color Internal monitor is ON and external monitor XGA output is ON Color adjustment for the internal LCD monitor SXGA LCD OFF Internal monitor is OFF and external monitor SXGA output is ON Color adjus
40. switch unplug the power cord and ask for repair 157 VIII Troubleshooting 2 Image Output 2 Image Output Symptom No image appears Only a crossline image appears on a white screen The image is out of focus The image is too dark or too bright Corrective action Connect the cables securely Connect the cables firmly in their specified locations Connect the DS camera head correctly Remove the C mount cap from the DS camera head Place the subject within the field of view of the microscope or lens Direct the optical path of the microscope to the camera Set the exposure correctly gt See 2 Photographing Images in Chapter VI Use a display compatible with the output format gt See 1 4 4 Adjusting Monitor Output for an External Monitor in Chapter VI Avoid selecting the external monitor output only gt See 1 4 4 Adjusting Monitor Output for an External Monitor in Chapter VI Connect the camera cable securely Set Sharpness on the CAM menu appropriately gt See 2 Photographing Images in Chapter VI Turn off the electronic zoom function Focus the microscope or lens correctly Do not use the system in a vibration prone place Adjust the aperture diaphragm correctly gt See Chapter V Microscope Adjustment The aperture diaphragm should be adjusted to approximately 70 to 80 of the numerical aperture of the objective or 70 to 80 of the pupil of the
41. the USB 1 1 interface board and USB 1 1 hub used in the system are compliant with USB 1 1 standards and that the USB 2 0 interface board and USB 2 0 hub used in the system are compliant with USB 2 0 standards Check that only one hub stage exists between the interface board and the DS L1 Use of only one hub stage is recommended Make sure the USB cables used in the system are USB compliant When the DS L1 is connected via USB 1 1 use USB 1 1 or USB 2 0 compliant cables When the DS L1 is connected via USB 2 0 use USB 2 0 compliant cables Check that the OS version used is compatible with the system Mac OS is not supported Supported OSs Windows 2000 Service Pack 3 SP3 or later Windows XP Make sure the USB host driver made by Microsoft Corporation is installed The driver included with the USB board does not work properly Images are updated once every 5 seconds Be aware that if files are loaded into a PC at intervals shorter than 5 seconds the captured image may not always be the latest one A warning in the task tray system tray such as HIGHT SPEED USB Device Plugged into non HI SPEED USB hub is displayed when you connect to a USB1 1 compliant USB port This is not an error and the DS L1 operates as a USB 1 1 device There may be a conflict between the drive letter assigned to the DS L1 and that of another USB device already connected to the PC Change the drive letter for the DS L1 referring to 5 3 2 Outline
42. the system administrator Settings Setting the FTP server 1 Select Apple Logo System Environment Settings 2 For Internet and network items in the System Environment Settings window click Shared 3 Select the check box Start FTP Service for Mac OS 10 1 on the File and Web tab or the check box FTP Service for Mac OS 10 2 on the Service tab Adding a user 1 In the System Environment Settings window click User for Mac OS 10 1 or Account for Mac OS 10 2 2 In the User window for Mac OS 10 1 or Account window for Mac OS 10 2 click New User and supply the User ID and Password that have been set for External Server of the DS L1 3 Close the window 4 For the IP address on the server side you can select the built in Ethernet through Apple Logo System Environment Settings Network Settings On home directory settings and the separation of save directories for each of multiple users DS L1is When a user is added a home directory is automatically created under the specified user name and directory name at a location below Macintosh HD Users When image files are transferred the FTP Dir specified on the DS L1 side is created at a position below this home directory and the image files are saved in that directory Example If the user name is DS1 and the FTP Dir specified on the DS L1 side is FTPIBOX then the FTP transfer directory is C Users
43. to the home directory When image files are transferred the FTP Dir specified on the DS L1 side is created at a location below this home directory and the image files are saved in that directory Example If the home directory is C DSFTP and the FTP Dir specified on the DS L1 Other settings side is FTPIBOX then the FTP transfer directory is C DSFTP FTPIBOX When enabling logon as an anonymous user In order to discriminate the user by which image files are written to the FTP server we in the above settings adopted a method to log in with a user ID and a password However it is possible to log in as an anonymous user 1 2 7 When setting External Server in Network Settings of the DS L1 select anonymous for the User ID and none for the Password Enable the FTP server PC for anonymous access As in home directory settings select the Allow Anonymous Logon check box on the Security Account tab in the Preset FTP Sites properties window Be aware that if you select the Allow Only Anonymous Logon check box no one but an anonymous user can log on 104 VI Basic Operations N ETWO RK 4 Using the DS L1 via a Network When specifying a directory for saving separate image files for multiple DS L1s that have been connected 1 Select a user in User Manager and specify a local path for the home directory in Profile The specified path should be below the home director
44. vine saan A 65 3A WAatiS an Overlay ooie a A A 67 3 3 Entering a Comment on the IMag ss sssssssnnsnsensnnnnannannnunnannannnnnnennennnnnn 68 Soi Frec Form DrawinG eaa a a a a ser euetaeee laces 68 3 3 2 Using the Count Marker to Count the Number of Points sssssasssrssssssnns 69 323 Entering Textual Comments eraai A E A E eet aleens 70 SA Meas ring OM ENE Sereen mirer aA E E A A EAEE 7i 3 4 1 Performing Calibration and Saving Values sssssssssssssssrrsrrnrrsrrnrrsrrrsnns 7i 3 4 2 Calling the Calibration Value ccccccccceece eee a E a EAE D 73 3 4 3 Measuring the Distance between TWO POINKS cccceeeeeeeeeeeeeeeeeeeeeeeenaaaes 73 3 4 4 Measuring perpendicular distance cc ccc eeeeeeeeee eee eeeeeeeeeeeaneeeeeenes 75 34 0 Measuring angles heeri rT r EENE 76 34 6 Meas ning Circles oaea a a a E a O 77 3 5 Adjusting the Subject Position and Comparing SIZES ccc ccc eeee eee eeeeeeeeeeeees 78 3 5 1 Using Crosshairs to Adjust the Subject POSition cccccceeeeeeeeseeeeeee ee ees 78 3 5 2 Using a Scale to Measure the Subject SiZe ccccccceec cece ee eeeeeeeeeeeeeeeeeees 80 3 5 3 Using XY Scales to Measure the Dimensions of the Subject cccceeeeeee eee 81 3 5 4 Creating a Translucent Image and Comparing It to a Live Image 82 3 5 5 Measuring vertical and horizontal diStANCeS ccccee cece cece cece eeeeeeeeeeeeeeees 83 Using the DS L1 via a INGEW Olas
45. 0 12 0 17 mm for No 1 0 15 0 18 mm for No 1 S ISO 8255 Thickness 0 13 0 17 mm for No 1 0 15 0 17 mm for No 1 H Cover glass Cut piece DANN S lt a N o o O o o o VE LL aA aa E E foi Slide glass 1 7 mm or less 2 The autofocus function may force contact between the sample and the tip of the objective resulting in damage to both For samples like the ones shown below adjust focus with the focusing handle rather than relying on autofocus e A sample with sealing agent rising excessively above the cover glass top surface e A sample whose sealing agent is damp not completely dry e A sample with sealing agent adhering to the surface of the cover glass e A sample whose cover glass is sticking out from the slide glass BAD GOOD eee ee eee Se ea aN A A SE AO ROO ZO A ZO O Z 153 MICROSCOPE VI Basic Operations 6 Using the Microscope Control Precautions for 901 setup 1 Make sure the observation method is set to bright field microscopy 2 Make sure the objective used is e N A 0 04 to 0 95 e x1 to x100 Phase contrast liquid immersion polarization Hoffman multi immersion and industrial use objectives may not be used 3 Make sure the objective information is set at each address on the nosepiece Other precautions 1 Autofocus relies on contrast at the center of the view field Thus the area of the target to be examined will not come into focus unless it is at the ce
46. 0 characters Pass Word Input up to 10 characters Once you set a user ID you will be asked to provide authentication when connecting to the DS L1 from a browser You cannot log in unless you supply your user ID and password If the user ID is defaulted to anonymous anybody can access your DS L1 via a network NOTE Note that if you save a password as the default word none without changing it the word none will become your password 88 VI Basic Operations N ETWO RK 4 Using the DS L1 via a Network Limiting connectable IPs If you want to limit the IP addresses connectable to the DS L1 select the IP Authority check box To register the IP addresses that are allowed to connect to the DS L1 follow the procedure described below Method for registering the IP addresses that are allowed to connect 1 Insert a CF card into the card slot of your computer NOTE Acard adapter may be needed depending on your computer 2 Create a directory named NET in the CF card 3 Create a text file that contains a list of the IP addresses that are allowed to connect to the DS L1 Description example 192 168 10 1 192 168 10 10 192 168 10 36 192 168 21 A wildcard can be used In the above example 1 to 254 are effective 4 Add an extension ip to the text file and save it in the NET directory ora directory hierarchically below it Example Autho ip 5 Insert the CF card into the DS L1 6 Connect to th
47. 1024 768 XGA The top and bottom 1 4 are not displayed The frame rate is 15 fps 33 2M cameras 2Mfull 800 6 800 5 C Scan VI Basic Operations 1 Preparations Before Photographing This display mode is useful when good performance with moving subjects is required Regardless of the source setting the CCD mode switches to match the shutter speed resulting in delays during image acquisition Progressive display modes eliminates color shifts even with moving subjects Cameras suitable for applications requiring a high frame rate and good motion performance An image area of 1600 1200 pixels is reduced for display at the monitor resolution 1280 960 SXGA or 1024 768 XGA The frame rate is 15 fps This display mode is useful when viewing images in great detail The CCD mode does not switch to match the shutter speed so there are no delays during image acquisition For this reason we also recommend this mode when making long exposures Progressive display modes eliminate color shifts even with moving subjects We recommend using this display mode as the general display mode An image area of 800 600 pixels is enlarged for display at the monitor resolution 1280 960 SXGA or 1024 768 XGA The frame rate is 20 fps This display mode is useful when good performance with moving subjects is required This mode is four times more sensitive to light than other modes making it is particularly useful for dark subjects
48. 2 Image File Settings File Settings Display a menu and select the SETUP tag on it to enter the SETUP menu Select File Settings from the SETUP menu to display the File Settings screen shown below PowerOn Settings NetWork Settings File Settings Additional Return to Window File Settings Pref ix Auto Create Card Dir I MGBOX Auto FFYYMMDD FTP Dir FTPIBOX Auto FFYYHHDD Sets a folder name in max 8 char preF ix 2 Year Honth Day which files are to be Pref ix Num Auto Create stored File Name DSLC Auto DDHHHHSS Sets a file name max 4 char Day Hour Hin Sec Log C with Los txt Enables or disables the File Type TYPE Le JPEG C pup log input function FIP Card SOURCE s5H L s1 34 L Auto change CCD mode CCD mode SIZE F Frame _ 25601920 12809960 _ 640 480 Sets a file format JPEG Quality L Fine Normal _ Basic L Draft waama POODE PMI MEI a bem screen for When you have finished setting each item press SAVE to save the data that has been set Or press Default to restore data to default values Card Dir FTP Dir Specify the folder name in which you want files to be stored For Card Dir specify a folder name used in a CF card For FTP Dir you can use up to eight characters to specify a folder name used in an FTP server If you select the Auto check box a folder is created automatically according to th
49. 9 CAM ERA VI Basic Operations 2 Photographing Images 2 o Photographing Images 24 Adjusting Camera Operation and Exposure for Better Picture Quality CAM Menu The CAM menu is displayed immediately after you display any menu or when you select the CAM tag The CAM menu has the following functions for setting photographing conditions e Four display modes are provided that are classified by resolution sensitivity live speed and balance You can choose the appropriate display mode according to the subject and photographic conditions only in the Advn menu e To obtain the optimum exposure of the camera you can choose or correct photometry or exposure mode e You can adjust the color and contrast 2 1 1 Simple Photography Easy Menu Select the EASY tab on the CAM menu and the Easy menu will be displayed The Easy menu allows you to photograph an image easily by only selecting the mode that suits the subject Configuration of the Easy menu Biological specimen scene mode Industrial specimen scene mode Displayed when the cooled camera is i connected Lev Lev Exposure level indicator Scene mode call button Custom mode call CMP Exposure compensation White balance AE L AE lock Metal Ceramic Focus Focus indicator Focus WWE 1520 Focus WE 1520 C Focus high sensitivity observation button Indicated in the body text as focus button o Exposure button Indicated in the body text as exposur
50. Briefly depress the mouse button to adjust the diaphragm in three increment steps NOTE The level indicator shows the current aperture of the diaphragm in increments of 0 4 in the range 2 0 to 8 9 a ND filter lt Adjusts the transmission factor of the ND filter gt Compatible only with Nikon 90i microscopes with motorized ND filters attached 1 In the ND filter column ail select the icon to increase the transmission factor by 3 relative to the current set value Or select the icon to reduce the transmission factor by 3 relative to the currently set value 2 Briefly depress the mouse button to change the transmission factor in ten increment steps NOTE The transmission factor changes from 2 to 100 fe Analyzer lt Inserting retracting the analyzer gt Compatible only with microscopes incorporating the DIH E lt Inserting the analyzer gt 1 If the analyzer is not inserted select the icon to move the analyzer into position 2 If the analyzer is in position the icon is marked with a red border lt Removing the analyzer gt 1 If the analyzer is inserted select the OUT icon to retract it 2 If the analyzer is not inserted the OUT icon is marked with a red border 147 M OOOO VI Basic Operations 6 Using the Microscope Control 6 2 3 Microscope Status Display Microscopy Setup Menu MIC Info Menu Right click anywhere on the screen where no other menu is displayed to display the microscop
51. C adapter Be sure to use a dedicated AC adapter Use of any other AC adapter may result in fire or electric shock 4 Power cord To prevent electric shock always turn off the POWER switch on the DS L1 when plugging in or unplugging the power cord For the DS U1 use only the specified power cord The specifications for the specified power cord are given below Using another power cord may lead to fire or other hazardous conditions Because this equipment falls under the category of electric shock protection class I always make sure it is grounded to a protective ground terminal If you misplace or damage the power cord contact your nearest Nikon representative e When using the product in 100 120 V AC regions Use an UL listed detachable power cord set 3 conductor grounding Type SVT No 18 AWG 3m long maximum rated at 125V AC minimum e When using the product in 220 240 V AC regions Use only an EU EN approved three conductor power cord set 3 conductor grounding Type HO5VV F 3m long maximum rated at 250V AC minimum 5 Handling e To prevent malfunctions or overheating due to short circuits do not allow this equipment or any other device used with it to become wet Never insert any foreign objects or materials into the equipment If the equipment gets wet or if foreign matter e g a scrap of paper gets inside the equipment turn off the DS L1 s POWER switch and unplug the power cord from the outlet Then contact your nearest Nik
52. E Use microscope control menu 1 to adjust the stage position longitudinally 149 Mite NOS eC y VI Basic Operations 6 Using the Microscope Control p Optical zoom lt Shows the optical zoom magnification gt Compatible only with microscopes incorporating the DIH 1 If the camera head is connected to the rear port of the DIH the menu indicates the magnification in the optical zoom column p for the current optical zoom incorporated in the rear port of the DIH NOTE Use microscope control menu 1 to change the magnification of the optical zoom If the a F Po is checkbox on microscope control menu 1 is checked zoom magnifications will not be displayed If the camera head is connected to the rear port of the DIH make sure the checkbox is unchecked pa Filter lt Shows filter name gt Compatible only with microscopes incorporating the DIH 1 Ohsery The menu indicates the name of the current filter in the filter column La NOTE x Before using this function you must set up filter information from a PC with the setup software For more information refer to the instruction manual for your microscope Use microscope control menu 1 to change filters FiFi Observation method lt Sets the observation method gt bserw Method E pic forc F FL Pn OF Te Bl i 16 If the microscope connected is a Nikon 90i microscope incorporating the DIH E you can use one of the Nikon specif
53. LAN is recognized The problem thus lies in the settings of the DS L1 or PC If a Request timed out message is returned the presence of the DS L1 is not recognized Check the cables hub connections and the network settings of the PC e g the IP address Also check that the PC is connected to a LAN by using a browser or the ping command E Browser Cannot access the e Set exception items in the browser s proxy settings camera from a browser Cannot control the e Deselect the Read Only check box in Network Settings of the SETUP camera from a menu browser E FTP gt See 4 6 Saving Images to an FTP Server PC from the DS L1 in Chapter VI Images cannot be Checking the set values saved to an FTP lt Settings on the DS L1 gt server e Make network settings in Network Settings of the DS L1 SETUP menu e g Enable Network and IP Address e Always be sure to make gateway settings if data communication between different network groups is to be performed such as sending or receiving data to and from a distant place of business lt Settings on FTP server PC gt e Check settings on the FTP server Check FTP server functions Check access rights Testing a connection login to the set server After making network settings on the DS L1 perform a ping test and login test 16027 Symptom Images are overwritten when saved to an FTP server The FTP send button cannot be pressed The Capture button d
54. M3806E 05 04 cr7 1 2 Nikon DS Camera Control Unit DS L1 DS Camera Head DS 5M DS 2Mv DS 2MBW DS Cooled Camera Head DS 5Mc DS 2MBWc lt Instructions gt Ver 3 0 Thank you for purchasing the Nikon products This instruction manual has been prepared for users of the DS Camera Control Unit DS L1 DS Camera Head DS 5M DS 2Mv DS 2MBW and DS Cooled Camera Head DS 5Mc DS 2MBWc To ensure correct use please read this manual carefully before using these products e The contents of this manual may not be reproduced in whole or in part without permission e The contents of this manual are subject to change without prior notice e Every effort has been made to ensure the accuracy of this manual If you have any questions or discover any errors in this manual please contact your nearest Nikon representative e Please refer to the instruction manuals for any other devices you may be using along with these products e g a microscope or personal computer Warning and Cautionary Symbols Used in This Manual Although Nikon products are designed to provide you with the utmost safety during use incorrect use or failure to observe the instructions may result in injury or property damage To ensure correct use read this manual carefully before using your products Do not discard this manual Always keep it at hand for easy reference Safety instructions in this manual are indicated by the symbols shown below To ensure safety please follow the in
55. Management Right click the volume whose drive letter you want to change and select Change the drive letter and path Click Edit and choose one of unassigned drive letters from the select box or enter a drive letter directly from the keyboard and click OK Click OK in the ensuing confirmation dialog box AEL USB VI Basic Operations 5 Using the DS L1 when Connected to a PC by a USB Cable How to discriminate between the media drive and the CCD image drive Right click any removable disk icon on the My Computer screen to display its Properties dialog box This dialog box helps you identify the drive assigned to that removable disk In the case of the CCD image drive for example it can be identified based on the volume label NIKONIMG and its capacity 16 0 MB as shown below Removable Disk H Properties 4 _ x General Tools Hardware Sharing Label NIKONIMG Type Removable Disk File system FAT Used space 366 640 bytes 360 KE o Free space 16 410 624 bytes 15 6 MB Capacity 16 7 79 264 bytes 16 0 MB Drive H cancel ano NOTE Note that because this drive is write protected its volume label cannot be altered 120 VI Basic Operations U S B 5 Using the DS L1 when Connected to a PC by a USB Cable 5 3 3 Using the Media Drive Sending receiving image files Insert media into the card slot of the DS L1 and double click the media drive removable disk icon on the
56. Nikon 80i microscope incorporating DIH E is connected ee 155 MICROSCOPE VI Basic Operations 6 Using the Microscope Control When the Nikon 90i microscope without DIH is connected 3 arts Menudisplayed U O O Nosepiece Motorized nosepiece Motorized universal Control status display menu displayed Universal condenser condenser Non motorized universal No menu displayed condenser Diascopic With motorized diaphragm Control status display menu displayed illumination aperture Without motorized diaphragm diaphragm Diascopic illumination field diaphragm Control status display menu displayed Episcopic illumination field diaphragm No menu displayed ND filter With motorized ND filter Control status display menu displayed No menu displayed Without motorized ND filter No menu displayed 134 VI Basic Operations MICROSCOPE 6 Using the Microscope Control When the Nikon 90i microscope incorporating DIH M is connected ee N e Nosepiece Motorized nosepiece Control status display menu displayed Shutter Control status display menu displayed Filter cube Status display menu displayed Motorized universal Control status display menu displayed Universal condenser condenser Non motorized universal l No menu displayed condenser Diascopic illumination With motorized diaphragm Control status display menu displayed aperture Without motorized i No menu displayed diaphragm diaphragm Dia
57. Normally average photometry should be selected The icon indicates the currently selected photometry mode Sample settings that suit different uses are described below Try these settings before determining your own settings There are few luminance changes over the entire screen that has a bright field Average photometry Whites on the background need to be whiter Peak hold photometry with exposure correction added The background is mostly black and the subject is sporadically bright Peak hold photometry Selecting a photometry area l Use one of the photometry area icons to specify a range of the screen in which you want the exposure to be adjusted Normally a wide photometry range is recommended To avoid influences from the periphery of the screen choose a narrow photometry range Displaying phoytometry area The photometry range is displayed with a red frame for a limited time when the ran right click again 4 Choosing tone When you set the tone the contrast will change Sample settings are shown below Confirm tones on the monitor when you set a tone and select the most suitable one To observe gradation in highlight reflections TONE1 Recommended example IC chips part mounting circuit board solder component leads gears etc To weaken or strength the standard contrast TONE2 weak or TONE4 strong Recommended example General photography To read the difference in luminous e
58. Other environment settings Return to window to return to the upper layer Shortcut Buttons for frequently used functions Freeze Electronic zoom AE lock Print Save to FTP server Save to CF card MIC INFO Settings of the frequently used status display and observation methods for the microscope Common icons on CAM VIEW REC TOOL and MIC menus The icons listed below can be used in all menu screens iO Resizes the displayed menu Closes the menu W Freezes the image F Electronic zoom Mp n 1 3 2 VI Basic Operations SETU P 1 Preparations Before Photographing Method of Displaying a Menu Follow the procedure described below to show or hide a menu or to switch between menus Using the MENU button on the main unit front panel Press the MENU button to display the CAM Easy menu To change menus select your desired tag with one of the cursor buttons and press the MENU button which functions as the enter button to confirm To close the menu screen press the button Using the mouse Right clicking with the mouse displays a menu The specific menu displayed depends on the mouse pointer position on the screen when you right click e Top 1 3th of the screen CAM Easy menu e Middle of the screen CAM Easy menu and shortcut menu e Bottom 1 3th of the screen Shortcut menu To close the menu screen right click anywhere outside the menu area Using the keyboard Pressing a function key on the keyboa
59. P gt Additional at the bottom right corner of the screen e If computer related problems occur Operating system version and service pack SP number Browser version PC specifications manufacturer model processor memory capacity etc e Peripherals USB mouse devices CF card drives etc Manufacturers model numbers e If image problems occur If possible provide a sample image 166 IX Technical Specifications 1 DS Camera Control Unit DS L1 Technical Specifications ao DS Camera Control Unit DS L1 Display LCD display 6 3 inch TFT liquid crystal display 1024 x 768 XGA External output Analog RGB output SXGA XGA selectable format SXGA 1280 x 1024 60 Hz non interlaced XGA 1024 x 768 60 Hz non interlaced Sync signal TTL 1 kQ Camera control Exposure control Program AE shutter speed priority AE focus AE manual Photometry Average photometry peak hold photometry photometry area size selectable Electronic shutter 1 1000 to 60 seconds for DS 5M DS 2Mv or DS 2MBW 1 1000 to 600 seconds for DS 5Mc or DS 2MBWc White balance Set method color balance adjustment Color correction Contrast black level saturation hue adjustment monochrome inversion other special effects are selectable Image correction Shading correction noise reduction horizontal vertical inversion of image Operation Scene mode Selectable from a total of six biological and industrial modes resetting possible Custom
60. S L1 and a PC 1 Turn on the power to the PC main unit and start Windows 2000 or Windows XP 2 Plug the A connector of the USB cable into the USB port of the PC main unit or the hub 3 Plug the B connector of the USB cable into the USB device connector of the DS L1 If this is the first time you are using the DS L1 in Windows the PC automatically recognizes the DS L1 and installs the device driver Refer to the subsequent pages for details NOTE This function is not available if a PictBridge printer is selected in the Additional Settings screen of the SETUP menu Before connecting the DS L1 to the PC confirm that the Mitsubishi CP900D printer is selected on the Additional Settings screen 2 Lis U S B VI Basic Operations 5 Using the DS L1 when Connected to a PC by a USB Cable 53 Using the DS L1 in Windows 2000 5 3 1 Installing the Device Driver When the DS L1 is connected to a PC for the first time the PC automatically recognizes the DS L1 and installs the device driver that comes standard with the OS During the installation process the window shown below will be displayed sequentially You do not need to perform any specific operations Found New Hardware 116 VI Basic Operations U S B 5 Using the DS L1 when Connected to a PC by a USB Cable 5 3 2 Outline of the Disk Drive When the DS L1 is connected to a PC the disk drive for the CF card etc and the internal image drive of the DS L1 are each
61. Specifications 4 DS AC adapter 4 DS AC adapter Rated input voltage Rated output voltage External dimensions Weight 100 240 VAC 50 60 Hz 1 2 A max 12 VDC 5 3 5 A max 121 L x 60 W x 35 H mm 350g 5 Overall Specifications Operating environment Temperature Humidity Altitude Pollution Installation Protection class Storage environment Temperature Humidity 0 to 40 C 85 RH max No dewdrops allowed 2000 m max Degree 2 Category 2 Class I Indoor use only 20 to 60 C 90 RH max No dewdrops allowed e The contents of this manual are subject to change without notice Conforming standards This product conforms to the UL60950 standard This product conforms to the EN60950 standard This product satisfies the FCC 15B Class A standard This product satisfies the EU EMC Directive This product satisfies the EU Low Voltage Directive This equipment has been tested and found to comply with the specifications for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area may ca
62. Supplement The method for inserting and removing a filter varies from microscope to microscope Refer to your microscope s instruction manual ii A third party color compensation filter CC filter can be inserted into the illumination optical path of the microscope to compensate for changes in color balance caused by the length of the exposure time f When using a phase contrast microscope or an interference microscope two luminous fluxes or multiple luminous fluxes you can enhance contrast using a green interference filter GIF or a monochrome interference filter IF i We recommend simultaneous use of an NCB filter and a green interference filter GIF for the DS 2MBW and DS 2MBWc a Some microscopes may require a heat wave absorption filter Terms What is an NCB filter An NCB neutral color balance filter is a color balancing compensation filter used to adjust color temperature to daylight values in microscopes that use a halogen bulb as a light Source What is a complementary color For example green and magenta red and cyan and blue and yellow are all pairs of complementary colors When complementary colors overlap their respective hues cancel each other out 17 7 V Microscope Adjustment 2 Adjusting the Field Diaphragm Adjust the field diaphragm so that it circumscribes the viewfield The field diaphragm has a significant impact on contrast especially for fluorescent specimens against dark b
63. You can set how long the DS L1 will wait before switching to power save mode Setting a value of 00 will disable the power save function Pointer speed Low Mid High Select the speed at which the mouse pointer moves across the screen when you move the mouse E Zoom Step Select the magnification rate of the electronic zoom at which rate the image is zoomed in or out Two or more choices can be selected Capture Function Save to Card Save to Server Print Select one of these functions to save to a CF card to save to the FTP server or to output to a printer you want assigned to the CAPTURE button on the DS L1 and the DS Remote Controller DS RC or to the CAPT button on the menu The CAPTURE button can be assigned two or more functions at the same time External Out This is an external interface output If you select ON a strobe synchronizing signal is output from the EXTERNAL OUT connector when capturing an image For normal use we recommend selecting OFF If you select ON a time lag will occur in external output with respect to the displayed image on the monitor Firmware Version The number at the bottom right indicates the firmware version The firmware version in the example shown is Ver 220 0202 1717 040825 This is the firmware Ver 2 20 300 KKK KX Or later version is the firmware compatiple with 2M 5M cameras Mp e n SETU P VI Basic Operations 1 Preparations Before Photographing 1 4
64. a drive removable disk icon on the My Computer screen and select Eject P My Computer x File Edit Yiew Favorites Tools Help a A D 3 O Back gt wi A Search Folders address TPE ae Shared Documents mi Administrator s Documents amp System Tasks view system information Hard Disk Drives TA Add or remove programs ne G Change a setting ee Con S Local Disk C 7 Local Disk D Other Places J My Network Places O Local Disk E es Local Disk F 3 My Documents O Shared Documents Devices with Removable Storage te Control Panel 4 314 Floppy A VY gt RIPFRE_EN G Details Qe My Computer So eT ae System Folder ge MATSUZAKI H ge NIKONING 1 Network Drives ap Psqdata on Psgsery T d ig v Remove the media manually from the DS L1 127 U S B VI Basic Operations 5 Using the DS L1 when Connected to a PC by a USB Cable 5 4 4 Using the CCD Image Drive Double click the CCD image drive removable disk icon on the My Computer screen to access the internal DS L1IMG folder of the DS L1 The latest image files that have been shot by the DS L1 are stored in the DS L1IMG folder and these files can be downloaded to the PC NOTE Because the CCD image drive is read only no image files can be sent from the PC to the DS L1 The image files stored in the DS L1IMG folder will have the file settings selected from File Settings of the DS L1 SETUP menu Example
65. ackgrounds Make sure you adjust the field diaphragm correctly For details on adjustment refer to your microscope s instruction manual 3 Adjusting the Aperture Diaphragm When using diascopic illumination Generally the numeric value of the condenser aperture diaphragm should be adjusted to approximately 70 to 80 of the numerical aperture N A of the objective When using episcopic illumination Generally the aperture diaphragm should be adjusted to approximately 70 to 80 of the size of the pupil of the objective that can be seen by removing the eyepiece and looking into the eyepiece tube Supplement For details on adjusting the aperture diaphragm refer to your microscope s instruction manual For photographed images that have greater depth of focus close down the aperture diaphragm Note that closing down the aperture may limit the performance of the objective Terms What is depth of focus Depth of focus refers to the range along the axis of the field of view over which the image is in apparent focus Although reducing the aperture diaphragm extends the depth of focus doing so will also reduce resolution Take this into consideration and adjust the aperture diaphragm in accordance with your needs 4 Adjusting the Focus Adjust the focus of the microscope so that the image can be clearly seen on the display 5 Adjusting the Lamp Voltage When photographing a color image the color reproducibil
66. agnification of the digital imaging head optical zoom to x1 0 before calibrating 1 Select the number in which you want to save the calibration value from through s7 Drag the mouse pointer over one of numbers through s7 and the calibration information for that number will be displayed in the Info area for your confirmation 2 Press to bring up a calibration screen like the one shown below 500 Com an Eom Con CTear set ie nto 3 Draw a calibration line about the length of a specific subject on the image that you want to be the reference Click the mouse button at the starting point of the object to be measured To draw a line hold the mouse button while you drag it to the end point then release the mouse button To redraw a line use to erase the line you ve just drawn 4 When you have finished drawing a line click the number input box to display a numeric keypad After entering any number press Ent 5 Select the unit of measure nm um mm cm 6 If you want to attach information to the calibration value you ve created click the Info input box and enter text for the information you want to attach This information will prove helpful because you can confirm Info when you call up the calibration value at a later time 7 Check the Set number to see if the calibration number is correct and press to save Unless you enter a numeric value and a unit you cannot press the button If you want to recalib
67. ained and you wish to save the created image in the internal memory press Save If you want to paste it to the overlay of the image use Paste If you do not want to paste use to erase the image before it is fixed You can use to display the superimposed image you saved in the internal memory 82 VI Basic Operations TOO L 3 Measuring Two point Distance or Entering Information TOOL Menu 3 5 5 Measuring vertical and horizontal distances You can measure the horizontal distance on the x axis and vertical distance on the y axis between two cross hairs you display on the screen 1 Right click on Cross The button will change to C Msr 2 Select the EJ C Msr icon Two cross hairs will be displayed on the screen To move a cross hair click the center or one of the lines and drag to the desired position Move both cross hairs near the object to measure the portion of the object between the horizontal and vertical axes The x axis distance and y axis distance are displayed near the center of the cross hairs 3 To change line colors press Color Seven colors are available red green cyan yellow magenta white and black 4 To clear the display press C Msr once again Normally the axes for measuring vertical and horizontal distances are displayed on the screen but not printed on or saved with images To apply axes to saved images or to print axes along with images select the Cross check
68. ard to the directory name file name file format and compression rate Refer to File Settings 1 4 2 Image File Settings File Settings in Chapter VI for details on the format in which images are saved You also can save or print images from a shortcut menu or the REC menu Refer to 2 2 1 Saving an Image to a CF Card REC Menu or 4 6 3 Saving Image Files in the FTP Server in Chapter VI for details on how to use the REC menu 2 1 2 Special Photography on the Easy Menu DF FL Scene Mode In DF FL scene mode the camera is operated differently from other scene modes Press the DF FL scene mode button and you will enter high sensitivity display mode for focus adjustment At the same time focus button and exposure start button are displayed First press focus button to adjust the focus When F focus button is right clicked the camera gain is increased to adjust the exposure automatically so that the exposure time will not be too long Then the live image is displayed while keeping the exposure status Left click on the KEycfocus button for manual mode NOTE Especially when the subject is extremely dark noise will stand out When the exposure adjustment is completed C_gn camera gain adjusting button and the shutter speed adjusting button are displayed By using these buttons the brightness of live image can be adjusted to your liking CICE CJH lLtyloyLloy ce After the focus adjustment press expo
69. are saved to the FTP server When images are sent via FTP the DS L1 does not check for duplicate file names Although file names are also suffixed with a serial number when Auto setting is not used if the power is turned off once the serial numbers recycle and start from 1 again Therefore it is possible that one of the existing files will be overwritten For folder names too selection of Auto for FTP Dir will help avoid overwriting files as a new folder is created for each date NOTE If the DS L1 remains turned off for more than a week its internal timer will stop If the DS L1 is not used for an extended period of time reset the time of day The backup function will recover when the system is powered on 112 VI Basic Operations N ETWO RK 4 Using the DS L1 via a Network 4 6 3 2 Using the CAM Menu to Save Image Files Select the REC tag at the top of the CAM menu screen to display the REC menu In the REC menu you can specify a directory and a file name before saving an image You can also choose interval photography which lets you shoot an image repeatedly at given intervals The following explains how to save images from the REC menu Configuration of the REC menu CAH VIEW REC JX TOOL SETUP Select the CF FTP check box and an image will be saved to both FY Press to save an image to the CF card vi cl Press to save an image to the FTP server Wad Dir FTOSO904 Press the directory name to the
70. as necessary and click the Save button When you click the Save button the image that is in the DS L1 is downloaded into the PC Because the latest image is downloaded it may not always the same as the preview screen Note that the Jpeg Quality pull down menu in the Pickup JPEG column allows you to select either Fine high picture quality or Basic low picture quality as the picture quality of the image when you save it The higher the picture quality the larger the file size will be 4 Interval Timer Set an interval at which the preview screen is to be updated Select your desired interval time from the pull down menu The display on the preview screen is automatically updated at the interval you set The set interval should be considered as a target interval If you select Off the preview screen will not be automatically updated 98 VI Basic Operations N ETWO RK 4 Using the DS L1 via a Network 5 Quality Set the picture quality of the preview screen The higher the picture quality the slower the display speed of the preview screen will be Select the quality according to your application 320 240 400 300 Low picture quality thinned out to 1 16 640 480 800 600 Middle picture quality thinned out to 1 4 1280 960 1600 1200 High picture quality not thinned out 6 E Zoom 7 Direction buttons 8 Sample image E Zoom allows you to turn the electronic zoom on or off When you select the E Zoom
71. at is formats not supported by the DS L1 are formatted in FAT16 For detailed information on using the formatting function refer to 2 2 2 Reproducing Images from a CF Card VIEW Menu in Chapter VI NOTE CF cards formatted in other than NTFS may not be correctly formatted in FAT16 depending on the formats used In such cases reformat those CF cards in FAT12 FAT16 or FAT32 on your computer before use Insert the connecting side with the connecting slot of a CF card into the card slot with the label side facing toward you Carefully note the direction and inclination of a CF card when inserting Check to see that the CF card indicator LED is off before inserting or removing a CF card Do not remove a CF card or turn off power to the DS L1 while the card is being accessed the LED indicator is on Attempting to do so may result in loss of recorded data or damage to the CF card My a SETU P VI Basic Operations 1 Preparations Before Photographing O13 Menu Configuration and Method of Displaying a Menu 1 3 1 Menu Configuration The menus available with the DS L1 are listed below Shortcut menus CAM Easy Advn Reg Camera setting menus VIEW Save to or reproduce from CF card two screen reproduction CF card thumbnail TOOL Menus for two point distance measurement and other tools SETUP Power ON Settings Startup settings Network Settings Network settings File Settings File settings Additional Settings
72. ata and comments using a mouse it s easier to enter such information with a keyboard Use a keyboard recommended by Nikon Not every USB keyboard is compatible with the DS L1 II Peripheral Equipment 4 USB hub You ll need a USB hub to use a mouse keyboard and printer at the same time Use a USB hub recommended by Nikon Not every USB hub is compatible with the DS L1 Only one hub can be connected to the DS L1 If your keyboard is equipped with a USB hub use this hub 5 USB printer Use the specified Mitsubishi CP900D or a printer which supports PictBridge Standard You must purchase a license to use the printer Contact your nearest Nikon representative for more information Restrictions will be displayed on the print screen if you attempt to use the printer without obtaining a license Refer to 1 4 5 Settings for Direct Print in Chapter VI for details on how to obtain a license 6 CF card A CF card is needed to record or reproduce photographed data to or from a CF card We recommend a Type I or Type II compliant CF card or CF Type II microdrive 1 to 4 GB 7 C mount lens A C mount lens is required to photograph subjects other than photomicrographs Any lens that satisfies the following conditions may be used e Protrudes no more than 10 mm from the mount surface into the camera e The image circle is 2 3 for the DS 5M and DS 5Mc and 1 1 8 for the DS 2Mv DS 2MBW and DS 2MBWc Use a lens for 2 3 or la
73. atically reappear Erasing the overlay Press Clear All and will appear asking you to confirm whether or not to erase Press if you want to erase or LN if you do not You cannot partially erase an overlay All pen drawings count markers text measurement lines and superimposed objects will be erased Crosslines and scales however will not disappear Saving or printing the overlay If you save or print an image while an overlay on it is being displayed the overlay image is written over the photographed image when it is saved or printed In this case the photographed image and the overlay are integrated into a single image when saved so you cannot erase the overlay from the saved image If the overlay is hidden while you save or print only the photographed image is saved or printed e Some text may be dropped when saving an overlay over an image if you save at the minimum file size Furthermore if the image is saved in JPEG format we recommend selecting a low compression rate such as Fine or Normal e For details refer to 1 4 2 Image File Settings File Settings in Chapter VI 67 TOO L VI Basic Operations 3 Measuring Two point Distance or Entering Information TOOL Menu 33 Entering a Comment on the Image You can enter a comment on the overlay of an image by using the pen drawing count marking or text function An overlay can be placed on top of a live image If you do not want the image and a c
74. ayed on the screen only and is not drawn on saved or printed images By leaving the Cross check box selected on REC menu you can draw crosshairs on saved or printed images NOTE T If while crosshairs are on you create or display a superimposed image the crosshairs will be hidden The crosshairs can be redisplayed after you have finished creating or displaying a superimposed image Crosshairs display at power on The DS L1 can be set to display crosshairs when it is powered on There are two ways to perform this setting 1 Setting from the SETUP menu Select ON for Crosshairs in PowerOn Settings on the SETUP menu and press SAVE 2 Using Telnet commands from a PC Crosshairs related Telnet commands are listed below w_CRSON1 Displays crosshairs at power on w_CRSON2 Displays a full size crosshairs at power on w_CRSOF Deselects display of crosshairs at power on w_CRSCOL color code Allows you to change the initial color of crosshairs RD red BL blue GN green PK pink WT white BK black YL yellow For information on Telnet commands refer to 4 5 Using Telnet Commands for the DS L1 in Chapter VI NOTE a The display position and display mode of crosshairs cannot be set using Telnet commands i Even if you ve set the DS L1 to display crosshairs at power on the crosshairs will be hidden if you choose to hide it or use a superimpose function If this happens redisplay crosshairs from
75. be displayed e IMG bmp e IMG jpg Here represents dimensional values such as 1680 or 1280 indicating image size The time of day for the files displayed at this time is O hours 0 minutes The actually acquired files are given the time of day when you issued the Get command 4 Execute the Get command for one of the files and the latest image that is in the DS L1 at that point will be downloaded to the PC A detailed command list is available from Nikon If you wish to have one please contact your nearest Nikon representative NOTE If frequent access is made to the DS L1 connected to a Windows PC via a USB cable image file acquisition may fail If this happens stop accessing the DS L1 via a USB cable for a while a Tol N ETWO RK VI Basic Operations 4 Using the DS L1 via a Network 45 Using Telnet Commands to Operate on the DS L1 You can use Telnet commands to operate the DS L1 from your PC NOTE Always be sure to consult the network administrator in your organization before executing Telnet commands Your organization s network may have been set up in a unique manner so invalid operations could cause a problem on your network Enter the IP address of the DS L1 from the MS DOS prompt command prompt of Windows to connect to the DS L1 Example Telnet 192 168 10 2 Commands Specify the commands listed in this manual A detailed command list is available from Nikon If you wish to have one pleas
76. box from the REC menu NOTE Creating or displaying superimposed images while measuring vertical and horizontal distances will stop measurement and hide the cross hairs You can resume vertical and horizontal measurement after creating or displaying the superimposed image 5 Normally values with these functions are labeled with the units nm um mm or cm However if calibration values are selected after inch mil conversion they are labeled mil and an E is indicated representing inch conversion 83 N ETWO RK VI Basic Operations 4 Using the DS L1 via a Network Using the DS L1 via a Network You can connect the DS L1 and a PC in a network and operate the DS L1 from the PC or exchange image files between the DS L1 and the PC There are three methods for operating the DS L1 from a PC e Use a WEB browser e Use an FTP command e Use a Telnet command You also can access an FTP server PC from the DS L1 via a network Possible operations in each method are described below The symbols and B in the diagrams below indicate the flow of images while the symbol gt indicates the flow of control Using a WEB browser to operate the DS L1 from a PC e Get images from the DS L1 e Control the DS L1 in a simple way A WEB browser may be used from a LAN based PC to observe images It is possible to view small images with a rapid updating speed by using Movie mode In this case the DS L1 s HTTP server functio
77. connection Eyera newak canecionie not present Al e dalm eeu connection GUrrenb Getault hone Bet WeTault M Beram system security check before dialing Local Area Network LAN settings LAN Settings gt Left click here 90 VI Basic Operations N ETWO RK 4 Using the DS L1 via a Network 6 Select Advanced If Advanced is disabled and you cannot select it left click on Use a Proxy Server first A check mark will be displayed allowing you to select Advanced Local Area Network LAND Settings Automatic configuration Automatic configuration may override manual settings To ensure the use of manual settings disable automatic configuration Automatically detect settings T Use automatic configuration script Address m Proxy server M Use a proxy server Address Bar Advanced l Bypass proxy server for local addresses Left click here me 7 In the Exceptions column write the IP address of the DS L1 Refer to 4 2 2 Setting Essential Items in Chapter VI for details on the IP address of the DS L1 e Enter a period after every three digits of the IP address e If the first digits of the IP address are 00 or 0 do not enter the zeros Proxy Settings m Servers T Use the same proxy server for all protocols Exceptions Do not use proxy server for addresses beginning with Enter the IP address here Example When ente
78. ction for the external monitor or via Display VGA LCD Color Adjustment in the Power On Settings screen Press SAVE to save your settings Turn the DS L1 back on again NOTE Because no images are displayed on the internal monitor when SXGA or XGA LCD OFF is selected you cannot work with the DS L1 without an external monitor after making these selections To change settings hold down the MENU button on the main unit while you turn on the power Keep the MENU button depressed until startup is completed Images will be output temporarily to the internal monitor Select XGA in Power On Settings and press i When using an LCD monitor or an LCD projector Adjust settings on the display side e g on the monitor or projector using the chart incorporated in the DS L1 Follow the procedure described below to make this adjustment 1 2 3 4 5 6 1 4 5 Connect an external monitor to the DS L1 Turn on the power for the DS L1 Give the unit about 20 minutes to warm up Also make sure the display has time to warm up sufficiently in accordance with the instruction manual Display a menu and select the SETUP tag on it to enter the SETUP menu Select Additional from the SETUP menu to go on to the Additional Settings screen Press the LCD Chart at the lower right corner of the screen A 1 dot pitched monochrome mesh chart will be displayed Adjust the clock phase and clock pitch on the display side R
79. cubes will change to the one that you ve selected 143 M OOOO VI Basic Operations 6 Using the Microscope Control 2 After switching filter cubes confirm that the selected number icon is marked with a red border and that the filter number and name are displayed NOTE Before this function can be used you must first set up filter cube information from a PC using the setup software For more information consult the instruction manual for your microscope If you are using a microscope incorporating the DIH M only the filter name is displayed Right click on an icon representing a filter number and the names of all registered filters will be displayed You also can select the desired filter from within this display to change filters The names of the filters in use are also displayed on the microscope status display microscopy setup menu MIC Info menu Lamp lt Controls lamp gt PON OFF Compatible only with the Nikon 90i microscope lt To turn the lamp on gt 1 When the lamp is off select the icon to turn the lamp on 2 While the lamp remains lit the icon is marked with a red border lt To turn the lamp off gt 1 When the lamp is on select the OFF icon to turn the lamp off 2 While the lamp remains turned off the OFF icon is marked with a red border lt Adjusting the brightness of the lamp gt 1 When the lamp is lit select the 9 icon and the lamp will become brighter in incr
80. d When you save an image in JPEG format it is saved to a file named after the image with the extension txt added Further it is embedded in the image file as Exif information NOTE If you select the check box labeled with LOG txt in File Settings of the SETUP menu the function to save a log comment statement along with an image is enabled In the REC menu you can dispense with this setting to attach a log when saving an image Overlay print Pen drawing text count marker and superimpose are saved overlapping on top of the image in the same position as displayed on the screen Scale and crosslines are saved according to the Scale and Cross settings on the REC menu If a Scale or Cross check box is selected scale or crosslines are saved in the image data When deselected they are not saved in the image data though displayed on the screen 50 VI Basic Operations 2 Photographing Images Saving an image To save an image to a CF card from the REC menu follow the procedure described below 1 2 3 Choose 1shot if you want to save only one image sheet Choose Continue to save multiple image sheets at given intervals Change the destination or file name to or in which to save as necessary If you want to save a log enter a comment in LOG Press CF the image will be saved to a CF card If you did not change the destination in step 2 the image will be saved using the directory name file
81. d is connected gt When you are using the DIH the camera head can be connected to the front port and rear port With default settings the camera head is connected to the rear port If you want the camera head connected to the front port when you use it select the check box MF Port by putting a check mark in it NOTE The F_Port check box is displayed only when the connected microscope incorporates the DIH M or DIH E i When the DS L1 is used with the front port you will see the message None displayed in the Zoom column of the microscope control menu 1 and the microscope status display microscopy setup menu MIC Info menu i Information on the selected port to which the camera head is connected is retained even after power to the camera head is turned off The saved settings take effect the next time the power to the camera head is turned on it Closed open lt Opens or closes the shutter gt Compatible only with microscope incorporating the DIH 1 Select the shutter change icon for episcopic illumination use to change the open closed status of the shutter 2 The current status is indicated by a picture depicting the shutter change icon i when closed ate when open ki Filter lt Changes filter cubes gt Fi 11E Ee fe Compatible only with microscope incorporating the DIH E 1 In the filter column z select the filter number to which your desired filter cube is registered The filter
82. de You also can confirm the information in the Info box NOTE The method for setting the contents of custom modes are detailed in the explanation of the Advn menu T Saved settings are applied to both 5M and 2M cameras However note that white balance WB will differ for 2M and 5M cameras a If you use several cameras of different types identify them with numbers 2 Adjusting the white balance Before photographing an image you need to adjust the white balance of the camera using a subject which is evenly white overall When using diascopic illumination for microphotography press WB while you are photographing a transparent part of the preparation and the white balance will be adjusted When using episcopic illumination for microphotography or using a lens adjust the white balance in the same way by using a white subject For fluorescent photographing we recommend that the white balance be adjusted under normal light conditions before photographing an image If the screen is excessively dark or if clipping occurs adjust the luminous energy of the light source or the iris diaphragm or use an ND filter to obtain the appropriate white balance NOTE For especially dark subjects noise may become conspicuous 3 Adjusting the focus Determine an image composition and adjust the focus while watching the image on the monitor If you have difficulty adjusting the focus because of a dark subject or there is an extended expos
83. detail The CCD mode does not switch to match the shutter speed so there are no delays during image acquisition For this reason we also recommend this mode when making long exposures Interlaced display modes may cause color shifts with moving subjects 1 3Mp An image area of 1280 960 pixels is formatted for the monitor resolution The image is displayed at actual size 1280 960 SXGA or reduced 1024 768 XGA The frame rate is 7 5 fps This display mode is useful when good performance with moving subjects is required If the source is set to s5M the CCD mode will switch to match the shutter speed resulting in delays during image acquisition Progressive display modes eliminate color shifts even with moving subjects We recommend using this display mode as the general display mode 1 3Mi An image area of 1280 960 pixels is formatted for the monitor resolution The image is displayed at actual size 1280 960 SXGA or reduced 1024 768 XGA The frame rate is 6 75 fps This mode is four times more sensitive to light than other modes making it is particularly useful for dark subjects If the source is set to s5M the CCD mode switches to match the shutter speed resulting in delays during image acquisition Interlaced display modes may cause color shifts with moving subjects C Scan An area corresponding to the center of the image is formatted for the monitor resolution The image is displayed at actual size 1280 960 SXGA or reduced
84. dure described below We recommended selecting Auto for the file name in File Settings so that files will be named after the photographed date and time 1 2 3 4 5 Press to change to Continue The Cnt and Int icons will be displayed In Cnt set the number of frames to be photographed Up to 500 frames can be set If Endless is selected in Cnt as the number of frames an image will be photographed successively In Int set a photography interval time It can be set anywhere from 10 seconds to 6 hours Choose the desired interval time from the following 10s 15s 20s 30s 45s 60s 1m30s 2m 3m 4m 5m 6m 10m 15m 20m 30m 45m 60m 1h30m 2h 3h 4h 5h 6h Press FTP and interval photography will start with images saved to the FTP server Press to stop photography in the middle Depending on the length of time required for network transfers the DS L1 may not be able to Save images at specified time intervals Prior confirmation will be necessary before you use interval photography If images cannot be saved at specified intervals During normal operation V Photographing WV Photographing WV Photographing Int set value Int set value Int set value Save Save Save When set intervals cannot be maintained V Photographing V Photographing Int set value Int set value Int set value Aa Saye eraic E DOV
85. e becomes red e Recommended example General photography of a relatively bright subject S_ AE Choose S_AE when you fix the electronic shutter while photographing the subject With the electronic shutter fixed to its set value the DS L1 automatically sets the camera sensitivity e Effective in cases where the brightness of the subject changes frequently while being observed under predetermined conditions and you are concerned with the response of automatic exposure e To photograph a moving subject while stopping it with an electronic shutter use this exposure mode in combination with 1 3Mp 2Mfull or 800 5 display mode e If fluctuations in exposure caused by movement of the subject presents a problem use to temporarily hold the exposure value During that time becomes red e Recommended example Stereoscopic microscope observation MANU Choose MANU if you are always photographing under the same exposure conditions or photographing special subjects In this mode the electronic shutter speed or the camera sensitivity is manually set in the Advn menu e The electronic shutter speed can be set from 1 1 000 s to 60 s for the non cooled camera and 1 1 000 to 600 s for the cooled camera e The camera sensitivity can be set from x1 C_gn 100 to x46 C_gn 4600 e Effective for fluorescent photography where careful consideration is required to determine the exposure as well as for subject
86. e contact your nearest Nikon representative 102 VI Basic Operations N ETWO RK 4 Using the DS L1 via a Network 46 Saving Images to an FTP Server PC from the DS L1 In this instruction manual explanations are given assuming that the IP address of the DS L1 is 192 168 10 2 that of the server is 192 168 10 1 and that of the subnet mask is 255 255 0 0 while the gateway address is 192 168 128 1 In practice you should use the IP address assigned to you by the network administrator in your organization It is assumed that network settings on the PC side LAN connection have been completed before this function can be used Please consult the network administrator in your organization for details 4 6 1 Setting the FTP Server Functions of the PC For image files to be transferred from the DS L1 you need a PC which has FTP server functions Only basic settings are described here Refer to your OS s help function and other materials for more detailed information on settings 4 6 1 1 When Using Windows NT Workstation Ver 4 0 Setup procedure 1 Install Microsoft Peer Web Services Add FTP server functions 2 Adda user User ID and Password input Set the DS L1 as the login user 3 Specify the home directory Specify the home directory when logged in 4 Enable writing to the server Enable writing image files to the FTP server Installing Microsoft Peer Web Services If Microsoft Peer Web Services is not
87. e status display microscopy setup menu MIC Info menu NOTE This menu is displayed only when the connected microscope is a Nikon 90i microscope or Nikon 80i microscope incorporating the DIH E Menu configuration when the microscope connected has all motorized parts attached Objective EMEA Shows magnification and NA value Stage position Z axis EEI Shows address along the vertical axis Z axis Yi Stage position X axis T il 26285m Shows address along lateral axis X axis Stage position Y axis Hic Info PRS op Fis i Ei Ohseryv Method 17191 um Shows address along longitudinal axis Y axis Optical zoom er Mete Shows the optical zoom magnification when r Filter i f2 3 4 75 6 connected to the rear port of the DIH E EE Shows filter name Balal Observation method BE Sets the microscope for bright field microscopy DIC Sets the microscope for differential interference contrast microscopy zje OIC FI Sets the microscope for differential interference contrast fluorescent microscopy FL Sets the microscope for fluorescent microscopy Ph Sets the microscope for phase contrast microscopy DF Sets the microscope for dark field microscopy lt User setup microscopy gt gt ais 6 Sets the microscope for user setup microscopy Info box information display indicates operating status or detailed items for the selected ic
88. e DS L1 during write operation follow the procedure described below 1 Eject the media from the media drive before removing the DS L1 2 Unplug the USB cable or turn off the power to the DS L1 3 When the below is displayed click OK Unsafe Removal of Device ajx fou have unplugged or ejected a device without stopping t Unplugging or ejecting devices without first stopping them can often cause your computer to crash and lose valuable data To safely unplug or eject any of the following devices first use the Hardware wizard in the Control Panel to stop the device USE Mass Storage Device EJ Generic volume IF pou frequently need to unplug this device Windows can give you an icon on the taskbar to quickly unplug or eject your device IF you would like to use this option check the following MV Show Unplug Eject icon on the taskbar Plug in the USB cable and turn on the DS L1 The device will be recognized by the PC again 124 VI Basic Operations U S B 5 Using the DS L1 when Connected to a PC by a USB Cable 5A Using the DS L1 in Windows XP 5 4 1 Installing the Device Driver When the DS L1 is connected to a PC for the first time the PC automatically recognizes the DS L1 and installs the device driver that comes standard with the OS During the installation process messages will be displayed sequentially in the task tray system tray at the lower right corner of the monitor screen indicating that
89. e DS L1 from the PC by Telnet and run the command w_IPA to register the IP addresses Refer to 4 5 Using Telnet Commands to Operate on the DS L1 in Chapter VI Saving logs of connected users NETLOG function A record can be kept of the IP addresses of the machines that accessed the DS L1 and of time information When accessed from a WEB browser access logs are kept only when the user IDs have been set in the Internal Server on the DS L1 side However these logs disappear when the DS L1 is powered off Run the Telnet command w_NLGON and the initial settings can be changed to display NETLOG To get the data contained in the logs access the DS L1 using an FTP command and get and read the file labeled NETLOG txt that is stored in the RAMIBOX directory or a directory hierarchically below it To get the data contained in the logs access the DS L1 using an FTP command and get and read the file labeled NETLOG txt that is stored in the RAMIBOX directory or a directory hierarchically below it NETLOG txt is up to 64 Kbytes in size If the buffer is filled the content is automatically erased and new logs are written to the file beginning with the 0 byte However if transfers to an external server have been set by the w_NLFTON command the log data is transferred to the external server before being erased The file name of this log data consists of the letters NL the date months days and hours and the extension txt W
90. e Easy menu In addition to scene modes custom modes can be called from through c7 You also can confirm the information in the Info box In the Advn menu you can save the set values of individual subjects and objectives in each custom mode 1 From the Advn menu set conditions such as camera operation mode and exposure NOTE Press to temporarily clear the set values revert to the initial values set when shipped from the factory 2 Select the REG tab to enter the Registration menu 3 Select the custom mode number from through c7 4 Click the Info input box and enter a character string A string can be entered using up to 16 characters 5 When done press to save If you enter the REG menu while AE_L is on you will see displayed in red To deselect AE_L before saving what you ve set press to unlock before you save The scene buttons can also be customized Although you can save all your own settings we recommend you call up the original scene once and apply correction to it before you save You can also use the Default to revert to the initial value 55 CAM ERA VI Basic Operations 2 Photographing Images 2 1 4 Special Photography on Advn Menu Fluorescent Photographing or Dark Subject Photographing The following settings are recommended for fluorescent photography or dark subject photography Settings on the CAM Advn menu Displayed only when the cooled camera is connect
91. e a measurement line to the overlay of the image press to fix after drawing it Press to return to the menu in which you previously were Only measurement lines cofirmed with will be saved Once the content is fixed as valid you cannot erase it unless you erase the entire overlay NOTE i The display information is interlocked with the menu size Small characters are displayed when you are showing a small menu and large characters are displayed when you are showing a large menu k Normally values with these functions are labeled with the units nm um mm or cm However if calibration values are selected after inch mil conversion they are labeled mil and an E is indicated representing inch conversion 7 If the microscope connected to your system is the Nikon 80i 90i microscope with motorized or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through s7 Calibration values are automatically changed when you change the objective 74 VI Basic Operations TOO L 3 Measuring Two point Distance or Entering Information TOOL Menu 3 4 4 Measuring perpendicular distance You can measure the distance between a reference line and a point on an image The measurement line for this measurement can be saved as part of the overlay for the image 1 Press Measure The tool bar shown below
92. e black level to the negative side with the SFT icon to prevent the black level from standing out When the cooled camera is connected Focus is displayed when exposure stops Click on lt SFT Display shown at end of exposure which automatically calculates the amount of black level shift only when the cooled camera is for negative side adjustment The amount adjuted is displayed connected in SFT 56 VI Basic Operations CAM ERA 2 Photographing Images Other settings required e In File Settings of the SETUP menu set the source to s5M or s2M high picture quality e In Additional Settings of the SETUP menu set External Out to OFF without shutter delay Check the screen for any abnormality If no problems are found save the image NOTE If you are photographing a dark subject that requires a long exposure time use the camera head in a cool place to allow for its temperature to decrease This will help to reduce star shaped noise and increase camera performance 2 1 5 Special Photography on Advn Menu Stroboscope Photographing When you photograph an image with synchronized lighting using a stroboscope plugged into the EXTERNAL OUT connector observe the following precautions as you set up the DS L1 e Select ON for External Out in Additional Settings on the SETUP menu and press SAVE This setting enables output of a synchronizing signal e The stroboscope is synchronized when th
93. e date on which you save an image The folder thus created is assigned a name that consists of the first two characters of your specified folder name and the six characters representing the date Example If the specified folder name in Card Dir is IMGBOX with the Auto check box selected and the date is 2005 03 28 then a folder named IM050328 will be created File Name Specify up to four leading characters for the name of the file in which you want to save an image A photographed image is saved under a file name comprising the specified file name and an automatically assigned serial number The serial number recycles back to No 1 when you turn off the power If you select the Auto check box a photographed image is saved under a file name comprising a total of eight characters representing the date and time days hours minutes and seconds at which you save the image To avoid duplicate file names we recommend selecting Auto under most conditions Example If the file name in File Name is DSL1 with the Auto check box selected and the date is 2005 03 28 15 30 54 the image will be saved under the file name 28153054 30 VI Basic Operations SETU P 1 Preparations Before Photographing Log If you select the check box labeled with LOG txt the function for attaching a log comment statement is enabled when you save an image Writing information such as subject name number and objec
94. e ei een E E EE EAE EEA EAEE 125 5 4 2 Outline of the Disk DiiVe wisisvcaaiaiad cties e A a R RAA Ei 126 54 3 Using the Media Drive sisi autora Ae Aare hee eee 127 5 44 Using the CCD Image Driver sscsives2ceeestsecceevesticenieseicaevesieaes seieaavesdienes es 128 5 4 5 Removing the DS LI from the PC 5 cn cicvccssdisvd can edsweessdisvdaeeeieeeasedieweaees 130 USINGi Tne MIChOSCODe CONE Oliriicat ci e A EA A AAN 131 Bal ON GIVICW mairo u n tana a nad a a E 131 62 MENU OVErVIECW usss reio ni a E EA ou sas EEE E E Rasa R ON 131 6 4 1 Microscope Control Ment Lermiisisi tiree a E en 137 6 2 2 Microscope Control Ment 2 ronen a a a hin 145 6 2 3 Microscope Status Display Microscopy Setup Menu MIC Info Menu 148 6 2 4 Ergo Controller button information display MenU ssssssssssrsrssrsrsrrsrerrne 151 6 4 95 Comnmecting a Motorized V EPirisnas a a 152 6 2 6 Connecting a Modular MICFOSCOPE sssssssssasnnnnannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 152 6 3 A tofoc s Precautions suiriri anA NU re E a r a aa 153 VII Care and Maintenance sessssesssssrsssesnenrrnerrnnrrrurrnrnrnnsnsnennrnrnrenrrenrrnrnno 156 i Cleaning the SYSECIM erone a A AA 156 PAE EEO are 18 R AE E TE E E T A A T AE T eee ee T 156 VILE TrOUBIESIOOUN O aaa TTA A mea uaAmeeemieawennnaaus 157 i JPOWCl SOUPDDI Y sak nacateaavatspaneraaesewoswaaas vvadaa I A AAN 157 2 AIMAGSOUC DUE sezcewsnccctcersesever a a a a 158 3 Photographing and SaviN
95. e other end to the D sub 15 pin connector on the display Secure the connections using a screwdriver to tighten the screws on the cable connectors oe hee IV Connecting and Installing the Equipment 3 Connection Methods 3 Connecting USB Peripheral Equipment Be sure to turn off the power for the DS L1 before making any connections When using a USB mouse USB keyboard USB printer Mitsubishi CP900D or Nikon 80i 90i microscope independently connect the device directly to the USB H connector on the DS L1 When using USB devices simultaneously connect them to a USB hub Only one USB hub can be connected to the DS L1 If your keyboard is equipped with a USB hub use the hub built into your keyboard 4 Connecting to a PC or a PictBridge Supporting Printer with USB Cable When connecting to a PC connect one end of the USB 2 0 cable AB type to the USB connector on the PC and the other end to the USB D connector on the DS L1 When connecting to a PictBridge supporting printer connect the PictBridge supporting printer and the USB D connector on DS L1 with a USB cable AB type 5 Connecting the DS Remote Controller DS RC Be sure to turn off the power for the DS L1 before making any connections Connect one end of the remote cable supplied with the unit to the EXTERNAL IN connector on the DS L1i and the other end to the TO EXT IN connector on the remote controller 6 Connecting to a Network Be sure to turn off power for the
96. e printers to perform the direct printing can be selected from either Mitsubishi CP900D or anyone which supports PictBridge Standard Mitsubishi CP900D selection mode Click the Mitsubishi CP900D check box to enter a check mark then click Save and turn the power for DS L1 off Connect CP900D printer to the USB H connector on DS L1 and turn the power on again This will enable printing by CP900D printer Or as another method connect CP900D printer to the USB H connector on DS L1 first then enter a check mark in the Mitsubishi CP900D check box and turn the power for CP9OOD on This also will make printing by CP900D printer possible When this mode is selected the function to capture the image on the PC by connecting the PC to the USB D connector on DS L1 remains enabled However even if a PictBridge supporting printer is connected to the same USB D connector printing by that printer is not possible PictBridge supporting printer selection mode Click the PictBridge check box to enter a check mark then click Save and turn the power for DS L1 off Connect a PictBridge supporting printer to the USB D connector on DS L1 and turn the power on again This will enable printing by the PictBridge supporting printer Or as another method connect a PictBridge supporting printer to the USB D connector on DS L1 first then enter a check mark in the PictBridge check box and turn the power for the PictBridge suppor
97. e shutter speed of the DS L1 is in the following range 5M cameras 5Mi mode 1 3 sto 1s 1 3Mp and 1 3Mi modes 1 7 sto 1s 2M cameras 1 15stols Select MANU for exposure mode and set the shutter speed within the above range e No noise reduction functions are supported Select NR OFF and leave it selected while in use e The DS L1i does not have an automatic exposure function for stroboscope Use the automatical brightness adjustment function of a stroboscope or select manual exposure in the DS L1 settings e Adjust the white balance from a frozen image following the procedure described below 1 While photographing a uniformly white subject press the freeze button to freeze the display At this time the stroboscope will flash Be careful to avoid overexposure 2 Press the button then deselect freeze e The stroboscope emits light when you press the CAPTURE button to save an image or the freeze button to freeze the display Freeze the display before saving an image this will allow you to check the photographic conditions NOTE Compared to ordinary photography there is a shutter delay in strobe photography because the stroboscope emits light only after you press the CAPTURE button sD CAM ERA VI Basic Operations 2 Photographing Images OoOo 22 Using a CF Card The DS L1 allows you to save a photographed image to a CF card You also can reproduce a saved image on the screen from the CF card 2 2 1
98. e start button o Image save button Indicated in the body text as Capt button NOTE focus button and Ey exposure start button are displayed only when DF FL scene mode is selected 40 VI Basic Operations CAM ERA 2 Photographing Images Photographing procedure To photograph an image from the Easy menu follow the procedure described below 1 Choose scene mode and custom mode Choose these modes according to the subject and the method of observation 2 Adjust the white balance Prepare an evenly white subject and adjust the white balance with WB 3 Adjust the focus Adjust the image composition and the focus 4 Correct exposure Adjust the brightness of the image with CMP 00 5 Confirm the image Press W freeze button to confirm the image During DF FL mode always use the E exposure start button 6 Save the image Save the image with Capt button or from the REC menu or shortcut menu Al CAM ERA VI Basic Operations 2 Photographing Images 1 Choosing scene mode and custom mode Choose a scene mode that suits the subject and the method of observation Scene mode To change the scene mode for industrial or biological specimens use Cam OSD on Power On Settings as selected from the SETUP menu e Industrial specimen scene mode Ind e Biological specimen scene mode Bio NOTE The scene mode setup values can also be set and saved as desired Instructions on how to
99. ease the frozen image press the button again DS Remote Controller DS RC Optional To EXT IN connector Connect the cable supplied with the unit from this connector to the EXTERNAL IN connector on the DS L1 CAPTURE button Saves image data A destination where image data is saved a CF card server via FTP or output directly to a printer can be assigned Above functions can be assigned to the button in Additional Settings of the SETUP menu IV Connecting and Installing the Equipment 1 Connection Diagrams Connecting and Installing the Equipment ao Connection Diagrams Schematic diagram DS L1 PC or a PictBridge supporting printer S USB be gt USB equipment mouse keyboard printer 5 gt and Nikon misroscope tanec gt LAN lt JA CF card DS AC adapter DS camera cable DS camera head 2 AO IV Connecting and Installing the Equipment 1 Connection Diagrams Connectors DS L1 Left side PC ora USB mouse supporting lt oe M E USB keyboard printer JE USB equipment LO i Printer oe Microscope ee Oo DS AC adapter I O LAN cable LAN DS L1 Rear 7 fied __ E i a O Ol EXTERNAL RGB OUT IN CAMERA G y RGB cable Remote cable Remote cable L L L External monitor DS RC DS camera head oe fe IV Connecting and Installing t
100. ed 1 Set the white balance under normal lighting conditions before photographing Adjust R and B Gain as necessary 2 Select small for the photometry area adjust for each subject 3 Select b average photometry for the photometry method adjust for each subject 4 When adjusting the focus first set the display mode to PROG 1 25Gs high sensitive 1 3Mi or 800 6 before proceeding Then press focus button You will have high sensitive and GMP press K image display With the 1 3Mi or 800 6 display GAIN mode high gain display is obtained CHR 00 HUE OFT changed to MANU By adjusting the shutter speed and camera gain manually fine adjustment of the displayed image can be performed lel c2 c3 c4 c5 c6 c7 6 Press exposure start button and the DS L1 starts pa Ti iH l automatic exposure When finished a screen is displayed with a EA frozen image Focus WH OSs on is displayed when exposure time is longer than 6 seconds Right click stops photographing 5 By that time the exposure mode has been automatically Example screen for DS 5MC 7 If necessary adjust the shutter speed and camera gain manually and press exposure start button again 8 The standard tone setting is TONE 3 but you can adjust it to your liking after the exposure Also the brightness can be fine adjusted by icon If the black level is found to stand out after photographing adjust th
101. een black white or yellow to set the color of the measurement line 3 Draw two lines tangential to the object to form the angle to be measured Click the mouse button at the starting point of the line To draw the line hold down the button as you drag the mouse Release the button at the end point The angle formed by the lines will be displayed Each angle is assigned numbers 1 to 9 The number of measurement lines drawn in the same color is limited to nine To change colors click another color icon so angles drawn in that color will be assigned numbers separately from those in other colors To redraw an angle use or to cancel or redo your most recent action Up to 10 recent actions can be retrieved Use to erase all the content you ve now entered All lines drawn before confirmation with will be erased NOTE If you draw a measurement line in the middle of or Redo recent measurement lines drawn from that point in time cannot be canceled 4 TO save a measurement line to the overlay of the image press to fix after drawing it 5 Press to return to the menu in which you previously were Only measurement lines and angles confirmed with will be saved Once the content is fixed as valid you cannot erase it unless you erase the entire overlay NOTE The display information is interlocked with the menu size Small characters are displayed when you are showing a small menu and large characters are displayed when you are sh
102. efer to the instruction manual provided with your display for detailed information on performing adjustments Some monitors have an automatic adjustment function Press to output a 16 level gray scale chart This chart can be used for reference when adjusting the output level or contrast of the display Once you have finished adjusting the display turn the DS L1 back on again Settings for Direct Print Before images can be output directly to a printer you must enter a direct print license first NOTE Contact your nearest Nikon representative for information on how to purchase a direct print license Follow the procedure described below to set a direct print license 1 2 3 Display a menu and select the SETUP tag on it to enter the SETUP menu Select Additional from the SETUP menu to go on to the Additional Settings screen If a direct print license has not been obtained the Printer column of the screen will be marked by a caution mark License Press License and an input window will appear Enter the license code in this input window and press the Ent This completes the settings You do not need to restart the DS L1 after making this setting 38 VI Basic Operations SETU P Preparations Before Photographing NOTE Ifthe message NG Code Error is displayed when you enter the license code check to see that the license code you ve entered is correct before retrying Th
103. el to the negative side Image level Gain Use this facility when fine adjustment in AE or MANU exposure mode has no effect Gain is referenced to Y100 100 and can be fine adjusted in increments of 5 The feature of this facility is that correction can be applied to a frozen image as well as to normal images Be aware that lowering the image level below Y100 causes the output saturation level to drop Color density CHRM To increase the color density in the image being displayed adjust CHRM to the positive side To reduce the density of color adjust CHRM to the negative side The density of color can be adjusted in the range from 50 to 50 Hue HUE Use this facility when you want to rotate hues in minute color adjustments DJ lt VI Basic Operations CAM ERA 2 Photographing Images Watch the image while you make adjustments Also be careful not to forget to reverse previous settings Effects COLOR B amp W NEGA BlueB Sepia You can choose color effects on an image from the following five choices COLOR This is the normal color image output B amp W This is a monochrome image Use this effect for monochromatic subjects such as DIC or PH and metal tissue Nega With the brightness reversed this is used as a special effect BlueBack Blacks in Nega are turned blue This is used as a special effect Sepia With colors developed in sepia this is used as a special effect Shading correction SD
104. ements of 0 1 V Conversely when you select the icon the lamp becomes darker in increments of 0 1 V NOTE The level meter indicating the brightness of the lamp changes in increments of 0 1 V from 1 0 V to 12 0 V Note however that the leftmost segment first step of the level meter indicates the voltage level from 1 0 V to 3 9 V lt Setting the lamp to the preset voltage 9 0 V gt 1 When the lamp is lit select the preset voltage icon FREE and the brightness of the lamp will be set to the preset voltage 9 0 V 2 While the lamp remains set to the preset voltage the preset voltage icon FREE is marked with a red border NOTE If you adjust the brightness of the lamp while it is set to the preset voltage the red border on the icon disappears 144 VI Basic Operations MICROSCOPE 6 Using the Microscope Control 6 2 2 Microscope Control Menu 2 Select tab 2 of the microscope control menu to display microscope control menu 2 This section describes the menus displayed on the DS L1 when the microscope connected is a Nikon 90i microscope incorporating the DIH E and having all motor driven parts attached full Spec If any of these components are missing or manual parts are attached no menus will be displayed for those components Menu configuration when the microscope connected has all motorized parts attached CAH VIEW REC GR condenser TOOL SETUP MIC ERE Shows condenser module name 4 Pha Selects the
105. emove Programs Add Remove Windows Components Select Internet Information Service IIS and click the Advanced button Examine whether the FTP File Transfer Protocol Services check box is selected If not select it and click OK After that follow the installation instructions You will be requested for a Windows 2000 or XP CD ROM during this course If you are using a preinstalled version it should normally be copied to a location below the System directory on drive C Thus you only need to specify the directory that contains the required files in order to have them installed If the files cannot be found other folders will be searched All of the following items can be set from Control Panel Administrative Tools Computer Management Adding a user Create a new user account 1 Select Computer Management Local System Tools Local Users and Groups 2 Right click on User to select New User 3 Enter a user ID and a password To ensure that the same information has been set for External Server in Network Settings of the DS L1 enter the user ID and the password within ten alphanumeric characters in lower or uppercase Deselect User must change password at next login check box NOTE You also can create accounts and set passwords from Control Panel Users and Passwords or User Accounts for XP Specifying the home directory and enabling write to it Enable image files to be writt
106. en from the DS L1 to the PC by FTP 1 Select Computer Management Local Services and Applications Internet Information FTP Sites 2 Right click Preset FTP Sites and select the Home Directory tab in the ensuing Properties dialog box 3 In Local Path for Directory at FTP Site specify the home directory or default home directory to which image files are to be transferred Also select the Write check box to enable writing to the home directory When image files are transferred the FTP Dir specified on the DS L1 side is created at a location below this home directory and the image files are saved in that directory Example If the home directory is C DSFTP and the FTP Dir specified on the DS L1 side is FTPIBOX then the FTP transfer directory is C DSFTP FTPIBOX NOTE The contents described above can also be set form Control Panel Administrative Tools Internet Information Services 106 Other settings VI Basic Operations N ETWO RK 4 Using the DS L1 via a Network When enabling logon as an anonymous user In order to discriminate the user by which image files are written to the FTP server we in the above settings adopted a method to log in with a user ID and a password However it is possible to log in as an anonymous user 1 2 When setting External Server in Network Settings of the DS L1 select anonymous for the User ID and none f
107. ent gradation during image processing 8 Saving the image Use Capt button or the CAPTURE button on the DS L1 to save the image E Capt button and the CAPTURE button on the DS L1 are functionally the same The image is saved to the destination that has been assigned to the button You can also use these buttons to print the image Look at the LED located by the side of the CAPTURE button on the DS L1 to confirm the destination to which the image will be saved 54 VI Basic Operations CAM ERA 2 Photographing Images NOTE The CAPTURE button can be assigned any desired function by the Capture Function on Additional Settings whether it be saving to a CF card saving to an FTP server or outputting directly to a printer Refer to 1 4 1 Date and Power save Settings Additional Settings in Chapter VI for details The image is saved according to the File Settings with regard to the directory name file name file format and compression rate Refer to File Settings 1 4 2 Image File Settings File Settings in Chapter VI for details on the format in which images are saved You also can save or print images from a shortcut menu or the REC menu Refer to 2 2 1 Saving an Image to a CF Card REC Menu or 4 6 3 Saving Image Files in the FTP Server in Chapter VI for details on how to use the REC menu 9 Saving photographing conditions Scene modes can be called up from the Advn menu in the same way as from th
108. exceed the limit Checking the set values lt Settings on the DS L1 gt e Make network settings in Network Settings of the DS L1 SETUP menu e g Enable Network or IP Address e Always be sure to make gateway settings if data communication between different network groups is to be performed e g sending or receiving data to and from a distant place of business lt Settings on a PC gt e When connecting from a PC that is not connected to a LAN check the network settings on the PC first e Set the IP address and other information specific to the PC Iols VIII Troubleshooting 5 Networking Symptom Corrective action Cannot connect the Checking for presence in a LAN system to a network lt Using a command from PC to search for the DS L1 in a LAN gt Continued from the Check network connections by using the ping command preceding page For Windows select Start Programs Accessories MS DOS Prompt in that order and enter the command from the prompt given below Windows 95 98 PC MS DOS prompt Windows NT 2000 XP Command prompt Mac OS X select Macintosh HD Applications Utilities Terminal and enter the command from the TERMINAL screen Example If the IP address of the DS L1 is 192 168 10 2 Enter the command ping 192 168 10 2 and hit the return key If a message such as Reply from 192 168 10 2 bytes 32 time is returned it means that the presence of the DS L1 in a
109. from this screen 2 3 4 6 9 1 Entrance ROOM2 Control Pad eee mentee Quality 3204240 640 480 1280 960 6 7 8 10 1 Tabs Entrance Returns you to the Entrance screen Reload Redisplays the ROOM1 screen with the preview image updated ROOM2 Displays the ROOM2 screen 2 Stream Window Displays a stream image subwindow in which a still picture is successively displayed as a stream image To close this window click the button at the upper right corner This window is automatically closed when you go to another screen Entrance or ROOM2 m amp Stream Microsoft Internet Explorer a ok e Method for displaying a live image Click the Play radio button to display a live image Click the Stop radio button to stop displaying a live image 96 VI Basic Operations N ETWO RK 4 Using the DS L1 via a Network 3 Control Pad Displays a subwindow for controlling the DS L1 From this window you can save the latest image to an image file You can also perform almost all operations that you would perform from the CAM Advn menu Refer to 2 1 3 Fine Adjustment before Photographing Advn Menu in Chapter VI NOTE No data or values can be entered directly from the keyboard Use a mouse to control the buttons on the WEB screen Control Pad Microsoft Inter m ba Enable Control Enable Control Sets or adjusts the DS L1 Be sure to deselect the check box labeled Read O
110. ge File Settings in Chapter VI When photographing a dark subject camera modes are automatically changed in order to maintain the picture quality while at the same time minimizing mistimed operation when the image is frozen If you are concerned with an unintentional change of camera modes set SOURCE to S1 3 DS 5M or S2M DS 2M in File Settings of the SETUP menu gt See 1 4 2 Image File Settings in Chapter VI 4 Abnormal Behavior Symptom The system s internal clock does not keep time correctly The keyboard or mouse does not work or it operates erratically Cannot write to or read from a CF card Corrective action If the system is not used for an extended period of time the backup function deteriorates causing the clock to stop Simply reset the correct time The backup function recovers when the system is powered on Be sure to use the recommended type of keyboard or mouse Check that only one USB hub is connected When using a keyboard that comes with a hub refer to 3 Connecting USB Peripheral Equipment in Chapter IV 3 Connection Methods for details on how to use a keyboard equipped with a USB hub Be sure to use a compact flash card Type I or II and a microdrive 1 to 4 GB Check that you are using a preformatted CF card or one that you ve formatted in FAT12 16 Sometimes these are expressed simply as FAT or FAT32 Depending on the OS used you may not be able to selec
111. giereirinean aaa E ORA 160 A sADMOMidiM Bera VIOL eirin A a AA A Ti 160 S NEWOFKIM O arrr trinan A A A A A AR 161 EA D E E AEI EEE E ATN EAA EN E AEE E ETN 164 7 Microscope Control from the DSL limirnar E ad N 166 S Before Contacting US 5 siniraan nb touaeinsaaalennn N T 166 X Technical SPeCiiCauiOns wirer none OEE N 167 t DS Camera Control UniP DS sl liidess oases an O a 167 De ADS Canora Heada a seers pecans ved oan kas aeemnatn Dae sea Maa eee ae aaie 169 VI CONTENTS 3 DS Remote Controler DSRC Sec scsaceccccesis seers eotnerensineiscorseneetecarern ineaseueenrss 4 DS AC adapter 5 Overall Specifications vil I Before Use 1 Types of DS Camera Head Before Use ao Types of DS Camera Head The following five DS camera head models are available The features of the DS camera head will vary depending on the model purchased Before use identify your DS camera head model The name of the model is indicated near the mount cap fitting position of the DS camera head Nikon No 5100 BIGITALSI GHT E E MADE IN JAPAN da w BY e DS camera head models and features DS camera head Image pickup device Cooling Body model name function color DS 5M 2 3 inch color 5 240 000 pixels DS 2Mv 1 1 8 inch color 2 110 000 pixels DS 5Mc 2 3 inch color 5 240 000 pixels DS 2MBWc 1 1 8 inch black and white Black 2 110 000 pixels For detailed informati
112. gital imaging head while using this function with the Nikon 80i 90i microscope connected to your system the screen display will reflect the zoom magnification X If you change the objective while using this function and if the microscope connected to your system is the Nikon 80i 90i microscope using a motorized nosepiece or intelligent nosepiece the screen display will change accordingly g If the microscope connected to your system is the Nikon 80i 90i microscope using a motorized nosepiece or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through Bal Calibration values are automatically changed when you change the objective 80 3 5 3 By displaying XY scales on the screen you can measure the approximate dimensions of the 3 Measuring Two point Distance or Entering Information TOOL Menu VI Basic Operations Using XY Scales to Measure the Dimensions of the Subject _ Onin ot subject Because the X and Y scales can be moved independently you can move the scales close to the subject before measuring its dimensions 1 2 3 4 5 6 Select the calibration value you want to use from through EA Press to display scales corresponding to the selected calibration value Point to part of the X scale and click the mouse button Hold down the button while you move the mouse
113. go Controller button information display menu To view button assignments on the Ergo Controller press the button on the Ergo Controller Compatible only with Nikon 90i microscopes connected to the Ergo Controller CAN VIEW REC GBB eo De pe erSrete otaqe speed gt NP CW NP COW Capture Capture Capture NOTE i Tab toggles back and forth between detailed settings for functions A and B This menu cannot be used to control the Ergo Controller or microscope 151 M OOOO VI Basic Operations 6 Using the Microscope Control 6 2 5 Connecting a Motorized U EPI You can connect a motorized U EPI to the DS L1 to switch objectives and filters to display the status of each objective and filter and to open and close the shutter 6 2 6 Connecting a Modular Microscope You can use the specified USB 232c adapter to connect a Nikon modular microscope to the DS L1 You can view information on the position of the modular microscope s motorized nosepiece and adjust the nosepiece position from the DS L1 menu NOTE ij Use only the specified USB 232c adapters for this function i Do not connect or disconnect the USB 232c adapter when the DS L1 is on jj Use the USB 232c adapter only when the modular microscope is connected The system will not function properly if nothing is connected or if instruments other than the modular microscope are connected 1 Use a USB 232c adapter to connect a modular microscope to the
114. h as image size differ from those of the 5M 20 VI Basic Operations SETU P 1 Preparations Before Photographing Preparations Before Photographing Using Various Input Devices 1 1 1 Using the DS L1 Panel Switches MENU button Cursor buttons ZOOM FREEZE CF CARD O PRINT LN O ZOOM button FREEZE button CAPTURE button MENU button menu enter button Pressing the MENU button brings up the CAM Easy menu While the menu is displayed use this button as the ENTER button to confirm your selection 7 n SETU P VI Basic Operations 1 Preparations Before Photographing Cursor buttons Use these buttons to select an icon in a menu or to increment or decrement a set value e Foran icon to increment or decrement a value such as the Gain icon on the CAM menu Position the cursor over the icon and press the MENU button to select the icon The color of the cursor frame changes to blue Press the right or top cursor button to increment the value or the left or bottom cursor button to decrement the value e Fora shutter speed The value changes in the same way as described above The right or top cursor button lowers the shutter speed to increase exposure whereas the left or bottom cursor button increases the shutter speed to reduce exposure e For a mode select icon such as one to choose an exposure method on the CAM menu Select an icon you want and use the right or top cursor button to select items in the f
115. h liquid crystal display you may need an external display if you want to observe images on a larger screen or at a higher 1 280 x 1 024 resolution SXGA Prepare an SXGA 60 Hz or XGA 60 Hz TTL synchronized display available for use with PCs For best results we recommend SXGA monitors SXGA 1 280 x 1 024 60 Hz non interlaced XGA 1 024 x 768 60 Hz non interlaced When shipped from the factory the equipment is set for XGA output If you ve set your equipment to SXGA to start up and are using a display that can only be synchronized to XGA timing hold down the MENU button on the DS L1 when powering up Be sure to hold down the MENU button until the DS L1 has started up This forcibly sets the DS L1 to internal monitor output and external XGA output modes when it starts up After the DS L1 has started up set to XGA in Power On Settings of the SETUP menu and save your settings NOTE If you are using an LCD display or LCD projector we recommend adjusting its clock phase and clock pitch 2 USB mouse You ll need a USB mouse to access functions available only from the mouse e g screen scroll while using the electronic zoom TOOL functions such as length measurement or the pen drawing function or histograms The mouse can also be used for various menu settings and operations Be sure to use a USB mouse recommended by Nikon Not every USB mouse is compatible with the DS L1 3 USB keyboard Although you can enter photographed d
116. he DS L1 adjust its output by the procedure described below 1 Connect an external monitor to the DS L1 Refer to Chapter IV Connecting and Installing the Equipment for details 2 Display a menu and select the SETUP tag on it to enter the SETUP menu Select Power On Settings from the SETUP menu to go to the Power On Settings screen 3 Press SXGA XGA LCD With each press the available choices will cycle in the sequence XGA LCD ON LCD Color SXGA LCD OFF XGA LCD OFF gt XGA LCD ON e XGA LCD ON LCD Color Internal monitor is ON and external monitor XGA output is ON Color adjustment for the internal LCD monitor e SXGA LCD OFF Internal monitor is OFF and external monitor SXGA output is ON Color adjustment for the external LCD monitor e XGA LCD OFF Internal monitor is OFF and external monitor XGA output is ON Color adjustment for an external LCD monitor e XGA LCD ON Internal monitor is ON and external monitor XGA output is ON Color adjustment for an external LCD monitor 237 4 5 VI Basic Operations 1 Preparations Before Photographing XGA LCD ON LCD Color is initially selected for Power On Settings When using an external monitor select SXGA LCD OFF XGA LCD OFF or XGA LCD ON Colors may not display correctly depending on the external monitor connected to the system If this is the case you can adjust color settings using the color adjustment fun
117. he Equipment 2 Installing the DS L1 2 Installing the DS L1 When installing the DS L1 on a flat surface such as a desktop by using the stand arm Extract the stand arm from the DS L1 and place it at an angle of 5 to 10 degrees as shown below Leave a clearance of about 100 mm around the DS L1 to prevent heat from collecting near the equipment When attaching a commercially available arm to the DS L1 Any commercially available VESA standard compliant 75 mm arm UL1678 suited stand and mount can be attached to the DS L1 When doing so make sure the following specifications are met DS L1 weight 1 3 kg Fitting screw hole M4 7 mm deep Tightening torque 80 120 N cm Leave a clearance of about 100 mm around the DS L1 to prevent heat from collecting near the equipment M4 screw holes Arm Side view of the DS L1 Rear view of the DS L1 12 IV Connecting and Installing the Equipment 3 Connection Methods 3 Connection Methods 1 Connecting the DS Camera Head Be sure to turn off the power for the DS L1 before making any connections Connect one end of the camera cable supplied with the unit to the CAMERA connector on the DS L1 and the other end to the CAMERA OUT connector on the DS camera head Squeeze the locking tabs on the connector as you plug in the connector When the connector is plugged in all the way the locking tabs return to their original positions locking the connector in place Mounting the
118. he IP address of the External Server that has been set in Network Settings of the SETUP menu cannot be found e Check the IP address of the server one more time e If images are sent across a gateway specify the Gateway address e Use the ping test function to confirm This is because the DS L1 has not logged in to the FTP server e Check that the User ID and Password on the DS L1 side and the user account on the server side match e Check settings on the server side to see if the FTP server function is active e Check settings on the server side to see if the server is enabled to write e If images are sent across a gateway specify the Gateway address e Use the login test function to confirm whether logged in e Check settings on the server side to see if the server is enabled to write 163 gt USB Symptom Data cannot be transferred normally Cannot be connected normally When images are successively downloaded it is the same image that appears on PC Warnings are displayed on the PC The DS L1 will behave as follows after being connected to a PC e Although a CF card is inserted into position a Please insert media message is displayed if you click the icon for the media drive eA similar message is displayed if you click the icon for the CCD image drive e Other USB devices already connected to the PC are disabled VIII Troubleshooting 6 USB Corrective action Make sure
119. he browser version To use a Web browser be sure to use a PC with the OS and browser recommended below Recommended OS Windows 98 NT 2000 XP Macintosh OS 8 6 9 2 X Browser Windows Internet Explorer 5 5 6 0 Netscape 6 2 7 0 Macintosh Netscape 6 2 7 0 e OS of the PC used for the FTP server and installation of an FTP server If images are to be saved in an FTP server from the DS L1 you need to have a PC with FTP server functions installed Make sure that the PC used for an FTP server is running one of the supported OSs listed below Supported OS Windows NT Windows 2000 Windows XP Professional Mac OS X If you are using Windows NT 2000 or XP Professional you must have the components for FTP server functions installed Check to see if those components are installed in your computer 86 VI Basic Operations N ETWO RK 4 Using the DS L1 via a Network 4 2 2 Setting Essential Items Network Settings In this instruction manual explanation are given assuming that the IP address of the DS L1 is 192 168 10 2 that of the server is 192 168 10 1 and that of the subnet mask is 255 255 0 0 while the gateway address is 192 168 128 1 Of course you should use the IP address assigned to you by your network administrator We must assume that network settings on the PC side LAN connection are complete Please consult your network administrator for details Network settings of the DS L1 If you are going to connect the DS L1
120. hen Using Windows 2000 Professional and XP Professional 105 4 61 03 VWoen Using Mac OS X sais assctatstavataweitinns Se auarew N N ENE 108 4 6 2 Setting External Servers of the DS L1 Network SettingS cceeeees 110 4 6 3 Saving Image Files in the FTP Server ccc cece cece eee e eee e eee eeeeeeeeeeeeees 112 CONTENTS 4 6 3 1 Using the CAPTURE Button to Save Image Fil S c cc cccceeee eee e eee ees 112 4 6 3 2 Using the CAM Menu to Save Image FileS ccccccssseeeeeeeeeeeeeeenaanaas 113 5 Using the DS L1 when Connected to a PC by a USB Cable ccccceceeeeeeeeeeeeee 115 5 1 Recommended Operating Environment cccccceeceseeeee eens eeeeeeeeeeeeeeeeeanaes 115 5 2 Connecting the DS Lih and aPC iraia a area Sande teniadenava ten eowlaudenste 117 5 3 Using the DS LI in WINGOWS 2000 wa csce esac cetatentaeadan tania eae eaaee aA 118 5 3 1 Installing the Device Drive sssri sinen n E a D iw eesiews seeweSh ons 118 5 5 2 Oucine OF Ne DISK DIVE riidasta tide piwedeatohrtadecansadeareartacesaaeds 119 533 USING the Media DIV E aria snus ead accraN enacts cad anual anaulus ead enavaameutwalatet 121 5 5 4 Using the CCl limade DING 2icci aka cteca a eee baka cae an ene aaa 122 5 3 5 Removing the DSI from the PC ws sesteszce teeta peaueaneee a eta eens 124 Soe USING ThE PSs li WINGOWS XP ipul tet er atewenanabenne E T E 125 5 4 1 Installing the Device Driver cuisn
121. hen transferred at 12 o clock on April 1 the file name would be NLO40112 txt Telnet commands for logs w_NLGON Displays NETLOG w_NLGOF Hides NETLOG Ss NLGCL Erases the content of NETLOG w_NLFTON Transfers NETLOG when the buffer is filled w_NLFTOF Does not transfer NETLOG when the buffer is filled 89 N ETWO RK VI Basic Operations 4 Using the DS L1 via a Network Operating the DS L1 from a WEB Browser 4 3 1 Setting the WEB Browser 4 3 1 1 When Using Internet Explorer 1 Start the PC Refer to your computer s user manual for details 2 Launch Internet Explorer Refer to your computer s user manual or the Internet Explorer help function for details 3 Select Tools Internet Options Left click here to open the pull down menu OL Ne K VICFOSOTT Le e Eile Edt View Favorites Tools Help f i La i A ae eea e 3 B a z Back morsad Stc Synchronize earch Favorites History Mail Size Print Eat Wind Updati Address E about blank TNR ARAE Show Related Links Internet Options Left click here 4 Select Connections 5 Select LAN Settings Left click here Internet Options General Security Content Connections Programs Advanced Use the Internet Connection Wizard to connect your computer to the Internet m Dial up settings Beings diala
122. hoose s5M or s1 3M For 2M cameras choose s2M or s800 For setting guidelines refer to Source Setting Guidelines Photography mode on the next page If you set the source to the highest resolution sSM for 5M cameras or s2M for 2M cameras and select Auto change CCD mode the display mode will adjust automatically to match the camera shutter speed For further details refer to Auto change CCD mode at the end of this chapter SIZE Setting the size of saved images For 5M cameras choose 2560 1920 1280 960 or 640 480 For 2M cameras choose 1600 1200 800 600 or 400 300 If you set the source to s800 and the display mode to 800 5 while using 2M cameras the actual image size will differ from the designated size Images will be saved at 800 560 resolution if you select 800 600 and at 400 280 for 400 300 This means portions of the image at the top and bottom will be cropped out Saving images as 400 300 JPEGs will produce 400 296 images cropping out a small portion of the bottom When using either 2M or 5M cameras and saving images for display from CF cards again the image size is changed to 1280 960 With 5M cameras images will still be saved at 1280 960 resolution even if the specified image size is 2560 1920 Likewise with 2M cameras images will be saved at 1280 960 resolution for a specified image size of 1600 1200 Specifying an image size of 800 600 will result in 640 480 resolution JPEG
123. iavhwasieitonoiin eiies eae tae tian ean A 40 2 1 Adjusting Camera Operation and Exposure for Better Picture Quality CAM Menu cccccceseeeeeeeeeseessnneeeeeeeseaneantesssesags 40 2 1 1 Simple Photography Easy Men Usnitiitoccus ocauainsd erin orto ervaineedananeeetee nae 40 2 1 2 Special Photography on the Easy Menu DF FL Scene Mode 00cceeee ees 44 2 1 3 Fine Adjustment before Photographing Advn Menu ssssssssssssssssssrssrssrens 46 2 1 4 Special Photography on Advn Menu Fluorescent Photographing or Dark Subject Photographing ccccccsececeeeeeeeceecceccnecceeceeeeceeaeenaeaaes 56 2 1 5 Special Photography on Advn Menu Stroboscope Photographing 57 jV CONTENTS 22 Usmo a CF Cat Giana eter erdiciniaea vad oe eatainis a ocd R nau Sudeeat endian E 58 2 2 1 Saving an Image to a CF Card REC Menu sssssssssssnnsnnnnnnnnnnnnnnnnnnnnnnnnnn 58 2 2 2 Reproducing Images from a CF Card VIEW Menu cccccccccceeee cece eee eee eeeeeee 61 2 2 3 Using Dual Window Display to Compare with the Reference Image 62 2 3 Outputting Images Directly to a Printer c ce cceeeceee seen ee eeeeeeesnenaneeesseeaaes 63 2 524 PAANO aM IMAG Cass veaceses tans des a se aamareacnaues ieeasanaie sau aAa 63 2 3 2 Various Print Related Settings cccccccceeee cece eee a aa 63 Measuring Two point Distance or Entering Information TOOL Menu 05 65 SL OHNE OF Ene TOOL MENU dirii
124. ied default observation methods 1 2 Select the icon representing your desired observation method to change microscope settings for this observation method The icon representing the current observation method will be marked with a red border NOTE The default observation method on startup is BF Right click on any icon representing an observation method to display the names of all observation methods You also can select the desired observation method from within this display to change observation methods 150 VI Basic Operations M 1 NOS OO 6 Using the Microscope Control lt To set the microscope for user setup microscopy gt 142 5 J4 5 Je Before using a specific observation method you must register it in the microscope To register the setting use the appropriate software installed on the PC connected to your microscope For more information on registering refer to the user s manual for the application If the names of observation methods are also registered along with their settings moving the cursor to one of the icons representing those numbers will display the name of the observation method under the cursor in the Info box lt To link the observation method with the photographing mode of DS L1 gt 1 Right click on the icon indicating an observation method to open the menu showing all observation methods ER 2 Enter a check mark in the check box MB CAH SCENE 6 2 4 Er
125. ion Servers Type Address of proxy server to Port HTP OoOo JQ Security ns io ETP ee Socks E boo Gopher PO i jo WAIS P bo r Exceptions Do not use proxy servers for domains beginning with 192 168 1 2 Use commas to separate Enter the IP address here Example When entering the IP address 192 168 1 2 7 Click OK Move the pointer to the position of OK and left click on it Repeat this until the screen returns to the one from step 1 93 N ETWO RK VI Basic Operations 4 Using the DS L1 via a Network 4 3 2 Using WEB Screens Once a PC and the DS L1 are connected through a network it is possible to check or capture the image that is being shot by the camera head of the DS L1 from a WEB browser Even from a distance you can check what image the DS L1 is currently shooting When you access the DS L1 from a WEB browser a WEB screen is displayed on which you can operate the DS L1 There are two WEB screens ROOM1 and ROOM2 available depending on your needs e ROOM1 Displays the image currently being shot at a specified interval Also allows you to control the DS L1 or display a stream image e ROOM2 Allows you to load saved image files from a CF card into the PC If multiple PCs simultaneously access one DS L1 through a WEB browser operation may slow down In the case of the simultaneous access from only two units of PC the operation should be comfor
126. ion You can display crosshairs on the screen and use it to adjust the center or the horizontal or vertical position of the subject 1 Press Cross and crosshairs will be displayed 2 Point to the center of the crosshairs and click the mouse button Hold down the button while you move the mouse The position of the crosshairs moves as you move the mouse 3 When the crosshairs position is where you want it release the mouse button to set that position 4 To change the size and color of the crosshairs press Color There are two crosshairs sizes a full size crosshairs and a center only crosshairs The color can be selected from seven colors red green cyan yellow magenta white or black 5 Press to bring up the tool shown below This tool allows you to select a display mode for the crosshairs that suits the subject whose position you want to adjust CAH VIEW REC Dx TOOL SETUP We Radius J20 40 x 60 e0 100 120 x 140 J160 180 200 220 240 J250 280 300 J320 340 360 Cross Line e Nermal Dotted Center Cross ejes No cae Jno e 6 Radius is an option that displays a concentric circle along with the crosshairs with respect to its center The numbers 20 to 360 in the Radius menu indicate the radius of the displayed concentric circle in pixel units Select the check box for any number to display a concentric circle whose radius corresponds to your
127. ions the stage gt NOTE VI Basic Operations 6 Using the Microscope Control If autofocus does not work correctly try moving the sample until a sharply contrasting part of the sample is at center of the view field Then reexecute autofocus Supported samples are specimen slide with cover glass Thickness of the Slide glass cut piece 0 9 mm to 1 7 mm both ends inclusive Slide glass of thickness of up to 1 2 mm are supported Compliant with JIS R3703 1998 or ISO 8037 Cover glass of thickness of up to 0 17 mm for No 1 and 0 18 mm for No 1 S are supported Compliant with JIS R3703 1996 or ISO 8255 Objective magnifications x1 to x100 NAO 03 to 0 95 are supported Phase contrast liquid immersion polarization Hoffman multi immersion and industrial use objectives cannot be used The supported observation method is bright field microscopy Before using the autofocus function please be sure to read Section 6 3 Autofocus Precautions Compatible only with Nikon 90i microscopes using a motorized stage 1 2 3 Set the coarse fine select icon to coarse mode The appearance of the arrow icons changes 3 indicate a setting to coarse or fine motion modes lt for coarse motion mode for fine motion mode Use the arrow icons to adjust the stage position To perform final fine adjustments set the coarse fine select icon to fine motion mode Use the arrow icons to adjust the stage position NOTE
128. itions Perform manual focusing if this happens Warn Exp Over Microscopy condition is too bright Focusing stops at the original position for the autofocus Use the autofocus function under following conditions set on the DS L1 when the sample is nearly in focus Scene mode BF Exposure time Under AE setting the exposure time should come within 1 120 to 1 350 in 1 3 MP display mode when the sample is nearly in focus Adjust with ND filters etc Warn Exp Under Microscopy condition is too dark Focusing stops at the original position for the autofocus Use the autofocus function under following conditions set on the DS L1 when the sample is nearly in focus Scene mode BF Exposure time Under AE setting the exposure time should come within 1 120 to 1 350 in 1 3 MP display mode when the sample is nearly in focus Adjust with ND filters etc Error In the event of an error one of the status codes OO FF will be displayed on the screen For more information refer to the 90i Microscopy manual Troubleshooting oat lo o VII Care and Maintenance 1 Cleaning the System Care and Maintenance 4 Cleaning the System e Use a soft cloth to wipe away dirt from the DS L1i the remote controller and the cables e Before cleaning the DS camera head first attach the C mount cap Then use a soft cloth to wipe away dirt e Use a blower to blow away dirt inside the DS camera head mount When d
129. ity of the image depends on the lamp voltage When a halogen lamp is used as the light source increasing the lamp voltage produces a bluish light while decreasing the lamp voltage produces a reddish light Except in cases where it is specifically necessary to adjust the tone the voltage should be set to the proper level for microphotography which depends on the microscope being used Since this level varies for each type of microscope refer to your microscope s instruction manual If the lamp voltage changes measure the white balance once again 18 V Microscope Adjustment 6 Adjusting the Exposure Time Use an ND filter to adjust the illumination for the microscope to a level that yields a suitable exposure time generally 1 15 to 1 250 s You can also adjust the camera sensitivity Terms What is an ND filter An ND filter is a filter that affects only the amount of light passed not the color balance of the light For example an ND2 filter cuts transmitted light in half while an ND16 filter reduces transmitted light to 1 16th of actual levels 7 Effects of Ambient Light When capturing a dark specimen with a fluorescent microscope for example ambient indoor light may enter the optical path Accordingly interior lighting should be darkened for photomicrography Place caps on the binocular eyepiece to prevent light from entering through the eyepiece 8 Effects of Vibration Because photomicrography involve
130. le eueiee ead eee 20 1 Preparations Before Photographing ccccsccsceeccecererenecceneseesscsaugevaesnseeeneneers 21 Le SUSING Various LNDUL DEVICES accisuicstadetuwadnaas vane aea ad a n aaa 21 LLL Using the DS L1 Panel SWItenes siira n eaves hee ecie ise eevee 21 1 1 2 Using the DS Remote Controller DS RC cccccccccceee cence eeeeeneeeneeeeeenees 22 te Lo USING ca MOUSE sissnsacacns aa enaussecaceets EENS 23 LLA Usmo a WEY DO ANG aieass c tet ncds coed chet ocd eens c et acd eras a N 24 12 NOteSs Oni Sing a CFCI Eao vite thd uw ann a a sense E A A a es 25 1 3 Menu Configuration and Method of Displaying a Menu cc ccc ceeeeee eee e eee eees 26 tacl Menu Configuratio sess sabe na cece cana Gost e a a a ccna Gest n a a 26 Lue Method of Displayihng a Me enunnasien aaa a a A A Maaaeaaanaes 27 LA MIG SOULS a ea E T E A E onmannny 28 1 4 1 Date and Power save Settings Additional Settings ssssssssssssrssrssrrsr 28 1 4 2 Image File Settings File SettingS ccc cece eee aE E ER E 30 1 4 3 Startup Condition Settings Power On SettiNngS ccccccccceeeeeeeeeeeeeeeeeenees 36 1 4 4 Adjusting Monitor Output for an External MONitor ccccccseeeeeeeeeeeeeeeenaes 37 14 5 Settings for Direct PrN asuissa ches cost det A a cae ctes coaieresccac cee ede 38 1 4 6 Settings for Connecting a MICFOSCOPE s sssssussnnsnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 39 2 PHOCOGrapilNG limMaG GS eisidas
131. les in the CF card The log comments displayed here are the contents of the log text that you attached from the REC menu by selecting Append when saving images e Method for downloading files 1 Choose any image file by clicking on its icon or file name and a screen will be displayed for you to select the folder in the PC to which to save 2 Choose the folder in the PC where you want to save the selected image file Change the file name before saving as necessary NOTE If you have difficulty opening an image file in the CF card that is inserted in the DS L1 directly from a PC on a network temporarily save the file to the PC first and then open it on the PC 100 VI Basic Operations N ETWO RK 4 Using the DS L1 via a Network 4a Using FTP Commands to Operate the DS L1 You can run FTP commands on your PC to get images from the DS L1 NOTE Always be sure to consult the network administrator in your organization before executing FTP commands Your organization s network may have been set up in a unique manner so invalid operations might cause a problem on your network 4 4 1 Using FTP Commands to Get DS L1 Image Files from a PC 1 Enter the IP address of the DS L1 from the MS DOS prompt command prompt of Windows to connect to the DS L1 Example FTP 192 168 10 2 2 Execute the Is command to search for a directory named RAMIBOX 3 Execute the cd RAMIBOX command The following virtual file names will
132. lue such as the Gain icon on the CAM menu Left clicking the icon increases the value while right clicking the icon reduces the value e Fora shutter speed Left clicking reduces the shutter speed to increase exposure right clicking increases the shutter speed to reduce exposure e For a mode select icon such as one to choose an exposure method on the CAM menu Left clicking lets you select items in the forward direction right clicking lets you select items in the reverse direction Some functions are available only with the mouse You will need a mouse to access all the functions of the DS L1 The following functions are not accessible without a mouse Electronic zoom scroll Screen scroll when split into halves Histogram display Pen drawing function Text input function Count marking function Two point distance measurement function including calibration Superimposed position adjustment ay ic ee VI Basic Operations 1 Preparations Before Photographing 1 1 4 Using a Keyboard You can use a USB keyboard with the DS L1 For frequent text or character entry you ll find using a keyboard most convenient Be sure to use a recommended type of keyboard Not all USB keyboards are compatible with the DS L1 Alphanumeric characters in upper or lowercase can be used with the DS L1 The function keys on the keyboard can be assigned various functions such as displaying a menu freezing or the Capture button for quick operations F
133. m the overlay data excluding the XY scale and the scale currently displayed is deleted For detailed information about the overlay data refer to the description of the TOOL menu When you change the optical zoom the XY scale and the scale currently displayed also are changed Seal nia Optical path change lt Changes optical paths gt Compatible only with microscope incorporating the DIH E 1 2 Select the optical path change icon to change optical paths The current status of the optical path is indicated by a picture depicting the optical path change icon a for Binocular ein for Front port Sn for Rear port 142 VI Basic Operations MICROSCOPE 6 Using the Microscope Control NOTE When you are using a microscope incorporating the DIH M only the current optical path is displayed i Each time you left click to select the optical path changes sequentially from Binocular to Front port and Rear port and back to Binocular When you right click to select the optical path changes sequentially from Binocular to Rear port and Front port and back to Binocular ii If you change the optical path AE is locked unless the port to which the camera head is connected has been set to be enabled If the rear port illumination is supplied at 30 70 from a connected DIH M the icon will change to indicate that the optical path has been switched to the rear port oy lt Selecting the port to which the camera hea
134. mage files Double click the CCD image drive removable disk icon on the My Computer screen to access the internal DS L1IMG folder of the DS L1 The latest image files shot by the DS L1 are stored in the DS L1IMG folder and these files can be downloaded to the PC NOTE Because the CCD image drive is read only no image files can be sent from the PC to the DS L1 The image files stored in the DS L1IMG folder will have the file settings selected from File Settings of the DS L1 SETUP menu Example IMG1280 bmp IMG1600 bmp For detailed information on file settings refer to 1 4 2 Image File Settings in Chapter VI NOTE j To capture files at maximum resolution 2560 1920 for 5M cameras or 1600 1200 for 2M cameras you must set the correct parameters for size and the correct display mode The display mode also needs to be set to maximum resolution 5Mi for 5M cameras or 2Mfull for 2M cameras Setting the display mode to a lower resolution will result in image captures at the lower resolution Do not try to capture image files while the display mode is set to C Scan Regardless of the file setting this display mode will result in an image constituting only a portion cropped from the center of the original image The image files stored in the DS L1IMG folder are updated once every 5 seconds Be aware that if files are read out at intervals shorter than 5 seconds you will not always get the latest image To confi
135. matically adjusted The white balance can also be adjusted from a frozen image 1 While photographing an evenly white subject press tT freeze button to freeze the display 2 Press while in this state and the white balance will be automatically adjusted 3 Press W freeze button again to release the frozen display thus completing your settings In case of over or underexposure the white balance cannot be adjusted precisely Be especially careful if you are performing exposure in MANU mode or using a low magnification objective To correct the automatically set white balance use the R B con for Gain to adjust the balance 6 Adjusting the picture quality The Advn menu has various functions for adjusting the picture quality Watch the monitor while you adjust the picture quality as necessary Sharpness SHPO to SHP5 A sharp video image will be obtained by enhancing the contours of the image The sharpness can be adjusted in six steps from 0 off to 5 strong Noise reduction NR ON NR OFF Selecting NR ON helps to reduce noise on the screen resulting in a clear image Lag or tails on moving objects may occur when NR ON is selected When photographing a moving subject or if an increased response during focusing is desired select NR OFF Black level SFT If you want to eliminate flare reflection in blacks on the screen or make blacks on the screen more distinct adjust the black lev
136. n refer to the explanation column of the CAM menu lt Using the autofocus function gt When this function is selected focus is adjusted automatically Before using this function please read the separate manual entitled 90i Reference Autofocus Also refer to Section 6 3 Autofocus Precautions in this manual 1 Select the autofocus AF icon JAF 2 The message Now Focusing will be displayed at the upper right part of the screen indicating that the autofocus function is being executed During this time the image display remains frozen 3 When autofocus is complete the message Complete is displayed and the frozen image display is updated to show the current image NOTE j While autofocus is underway you cannot use any other menu After autofocus is complete a completed status code may be displayed For detailed information about the code refer to the instruction manual for your microscope If this is the first time you have used autofocus after replacing samples make sure the magnification of the objective is x4 or less At higher magnifications the autofocus range is narrowed Make sure the target to be inspected is at center of the view field i Use the autofocus function under scene mode BF display mode 1 3MP and exposure mode PROG 1 120 1 350 Make sure the focus indicator is about 350 or more when the sample is approximately in focus Loo Mite NOS eC y Motorized stage lt Reposit
137. n closed Then when the exposure by the exposure start button is completed the shutter closes When performing the focusing again with the focus button or performing the exposure again by the exposure start button the shutter opens again Further when selecting another scene mode or the custom mode to get out of the scene mode the shutter opens in case the shutter has been closed NOTE When using Nikon 80i microscope equipped with DIH M if the automatic shutter open close function is required input the following Telnet command from the PC w_DEPION2 With this operation the shutter will always open close automatically while the microscope is used in the DF FL scene mode NOTE If you are photographing a dark subject that requires a long exposure time use the camera head in a cool place to allow its temperature to decrease This will help to reduce star shaped noise and increase the camera performance 45 CAM ERA VI Basic Operations 2 Photographing Images 2 1 3 Fine Adjustment before Photographing Advn Menu Select the Advn tab on the CAM menu and the Advn menu will be displayed In the Advn menu you can make various settings of the camera individually to obtain an optimum combination of settings for the intended subject Configuration of the Advn Advanced menu Displayed when the cooled camera is connected P Focus high sensitivity observation button Exposure button
138. n is used NOTE As a rule although it depends on how frequently images are updated the DS L1 can only be accessed from up to two PCs at the same time 84 VI Basic Operations N ETWO RK 4 Using the DS L1 via a Network Using FTP commands to operate the DS L1 from a PC e Get images from the DS L1 e Send and save images to the DS L1 e Advanced control of the DS L1 rea Ld FTP commands may be used from a PC to receive send or save image files Furthermore advanced control of the DS L1 is possible using the DS L1 s FTP server function Using Telnet commands to operate the DS L1 from a PC e Advanced control of the DS L1 Telnet commands may be used from a PC to achieve advanced control of the DS L1 In this case the DS L1 s Telnet server function is used Operating on a PC from the DS L1 e Send images to an FTP server Images can be transferred to a specified FTP server NOTE This function cannot be used unless an FTP server facility is available on the PC side Because up to five FTP servers can be specified images can be easily and quickly filed in a personal directory created separately one for each login user Furthermore when this function is combined with image file base software a more efficient image filing system may be created In this case the DS L1 s FTP client function is used 85 N ETWO RK VI Basic Operations 4 Using the DS L1 via a Network 42 Network Setting
139. n to check the image in the DS L1 before downloading it into the PC When you change the size of the window the size of the preview screen changes accordingly 99 N ETWO RK VI Basic Operations 4 Using the DS L1 via a Network Using ROOM2 ROOM2 displays a list of image files saved to a CF card The image files can be downloaded from here to the PC 4 3 2 1 DS L1 Entrance ROOM1 Reload C IM030306 Name Date Time Comment Lip G Imo30306 06162510 JPG 2003 03 06 16 25 10 photol 5 Tos 06162543 PG 2003 03 06 16 25 43 photo2 06162613 JPG 2003 03 06 16 26 13 photo3 B 06163118 JPG 2003 03 06 16 31 18 nikon datal 1 Tabs Entrance Returns you to the Entrance screen ROOM1 Displays the ROOM1 screen Reload Redisplays the ROOM2 screen 2 Location The DS L1 installation information etc that has been set by Telnet commands is displayed here Refer to Using ROOM1 described above for details on making these settings 3 Current folder name Shows the current folder name with the absolute path 4 Folders in the CF card Shows all folders in the CF card from the top level folder down to the current folder Click any folder icon or folder name and the folder at the level directly below it and the JPEG format files and BMP format files in that folder all are displayed 5 Files in the CF card Shows the file names created date time and log comments of the JPEG format files and BMP format fi
140. name file format and compression rate that have been set in File Settings Refer to File Settings 1 4 2 Image File Settings File Settings in Chapter VI for details on the format of the saved image Interval photography Interval photography refers to when an image is photographed successively at specified time intervals To perform interval photography follow the procedure described below We recommended selecting Auto for the file name in File Settings so that files will be named after the photographed date and time 1 2 3 4 5 Press to change to The Cnt and Int icons will be displayed In Cnt set the number of frames to be photographed Up to 500 frames can be set If Endless is selected in Cnt as the number of frames an image will be photographed successively In Int set an interval time ranging from 10 seconds to 6 hours Choose the desired interval time from the following 10s 15s 20s 30s 45s 60s 1m30s 2m 3m 4m 5m 6m 10m 15m 20m 30m 45m 60m 1h30m 2h 3h 4h 5h 6h Press CF and interval photography will start with images saved to an CF card Press to stop photographing in the middle Depending on the length of time required for writing to a CF card the DS L1 may not be able to save images at the specified time intervals Prior confirmation will be necessary before you begin interval photography If images cannot be saved at specified intervals D
141. nergy from the photographed image data TONE5 50 VI Basic Operations CAM ERA 2 Photographing Images e To Suppress unevenness in bright parts while photographing an image with clearly distinguishable dark and bright areas TONE6 Recommended example Metal tissue or ceramics and other materials photographing documents with a lens etc e To enhance contrast for easy recognition gt TONE7 Recommended example Metal tissue or ceramics and other materials photographing documents with a lens etc Features of tones There are seven kinds of tones Choose an easy to observe tone according to the subject TONE1 Wide dynamic range Use this tone when gradation from darks to brights is required as for subjects that have highlight reflections Recommended example IC chips part mounted circuit board gears etc TONE2 Weak contrast Use this tone when you want to weaken the contrast Recommended example Cells and tissue general subjects TONES Standard contrast Use this tone for ordinary photographing Recommended example Cells and tissue general subjects TONE4 Strong contrast Use this tone when you want to enhance the contrast The image will have clearly distinguishable darks and brights Recommended example Cells and tissue general subjects TONES Linear There is a linear output proportional to the input from the camera Use this tone when you want to examine the difference in luminous energy from ph
142. nly FTP Cone 7 WEB disable Write Control in Network Settings of the SETUP menu of the DS L1 before making any settings here TONE Y_GAIN 100 Program G_GAIN 100 B_GAIN a 100 Sharpness 3 Download BMP Download BMP Downloads an image file in BMP format Jpeg Quality Normal Pick up JPEG Gets an image file in JPEG format To close this control pad click the button at the upper right corner This control pad is automatically closed when you go to another screen Entrance or ROOM2 e Download BMP Click the image size you want to download and the latest image in that size will be downloaded into the PC in BMP format 1 When you click any image size to select Save it to disk a screen is displayed for you to select the folder in the PC that you wish to save to Save in Nikon El fa L Nikon 1 Nikon L Nikon10 J Nikon L Nikon2 C Nikong C Nikon3 C Nikon File name IMGMID Save as type Bitmap Image 7 Cancel ZA 2 Choose the folder in the PC where you want to save the image file Enter a file name as necessary and click the Save button When you click the Save button the image that is in the DS L1 is downloaded into the PC Because the latest image is downloaded it may not always be the same as the preview screen s07 NOTE VI Basic Operations 4 Using the DS L1 via a Network If you click the Cancel button in the folder select
143. nter button or key Erasing images Locate the file name you want to erase at the gt position and press erase button The image will be erased Displaying a thumbnail Locate the folder name whose thumbnail you want to display at the gt position and press thumbnail display button A thumbnail of the folder will be displayed Place the mouse pointer on one of thumbnail images and the playback delete FTP save and print buttons will be displayed If the folder contains more than nine image files you can change pages using the lt or gt direction buttons NOTE Right click on the mouse if you want to stop a thumbnail from appearing in the middle 61 CAM ERA VI Basic Operations 2 Photographing Images Using the formatting function If you insert a CF card formatted in NTFS i e formats not supported by the DS L1 the following message will be displayed on the VIEW menu Error Unacceptable CF Card Format FAT16 Format Press the button to format the CF card in FAT16 format NOTE CF cards formatted in other than NTFS may not be correctly formatted in FAT16 depending on the formats used 2 2 3 Using Dual Window Display to Compare with the Reference Image A FREEZE Live You can split the screen into left and right halves and display a still image in the left half and a live image in the right half for image comparison You can use the electronic zoom to zoom in or out in ei
144. nter of the view field The microscope system may have difficulty focusing accurately on samples like the ones indicated below e Low contrast samples e Samples densely dyed dark over the entire view field e Samples prepared for methods other than bright field Detection microscopy range e Samples that lack light and dark patterns running horizontally along the screen lateral direction in the diagram shown at right 2 Since autofocus responds to the highest contrast part of the sample the focused position of the sample may not always be the area you want to observe In such cases adjust focus visually by turning the focusing handle of your microscope 3 The autofocus may occasionally misfocus due to external disturbances If the image fails to come into focus try autofocusing again 4 The microscope system may operate erratically if dirt or dust accumulates in the following areas e Top or bottom faces of the slide glass e Tip of the objective e Surface of the condenser e Inthe middle of the optical path 154 VI Basic Operations MICROSCOPE 6 Using the Microscope Control List of autofocus messages The following messages may be displayed while the microscope is autofocusing Now Focusing Now autofocusing Do not alter the microscopy conditions Complete Autofocusing complete Comp Low Contrast Sample lacking sufficient contrast for autofocusing Slight focal difference may take place in some cond
145. nterface board or a USB 1 1 hub the DS L1 units do not operate normally If connecting multiple units of DS L1 is desired use a USB 2 0 interface board and a USB 2 0 hub or use Windows XP for the OS USB cables The DS L1 does not come with a USB cable Be sure to purchase an appropriate cable that suits the USB standard of the PC or hub that will be used e USB 1 1 or USB 2 0 compliant cable when the PC or hub is USB 1 1 e USB 2 0 compliant cable when the PC or hub is USB 2 0 The DS L1 may not operate reliably if you use cables other than those specified 116 VI Basic Operations U S B 5 Using the DS L1 when Connected to a PC by a USB Cable USB host drivers Use the USB host drivers made by Microsoft Corporation as the drivers included with your interface board may not operate properly e When using the USB 1 1 interface board The driver is included with the OS e When using the USB 2 0 interface board The driver is included with the OS providing it is Windows XP Service Pack 1 SP1 or newer If you are using Windows 2000 or Windows XP prior to SP1 download the USB 2 0 driver from the Microsoft Windows Update Web site Visit the Microsoft website for details on how to download drivers To install the USB 2 0 host driver that you have downloaded read the manual supplied with your interface board or consult the manufacturer 52 Connecting the DS L1 and a PC Follow the procedure described below to connect the D
146. nu 58 VI Basic Operations CAM ERA 2 Photographing Images In the REC menu you can specify the photographing source file format image size and compression rate when saving an image e Choose the source either S5M for 5M cameras or s2M for 2M cameras to obtain high picture quality NOTE Refer to Outline Guide to Source Photographing Mode Settings in 1 4 2 Image File Settings File Settings Chapter VI for details about the source e For the file format choose either JPEG or BMP e If you choose JPEG for the file format choose the compression rate picture quality from Fine Normal Basic or Draft e Choose the image size At startup settings that were made in File Settings of the SETUP menu are effective However your settings in the REC menu remain effective until you turn off the power In the REC menu you can further save a comment or overlay a print along with the image Comment Select the Attach Log File check box and enter a comment in the input box That way you can save photographing information or comments in text format up to 100 characters at the same time you save an image You may enter the subject name number and the objective magnification for example to facilitate confirmation at a later time When you save an image in BMP format the comment is saved to a file named after the image with the extension txt adde
147. o maximum resolution s5M for 5M cameras or s2M for 2M cameras This mode lets you observe bright subjects at high speed or dark subjects at high resolution without manually adjusting the display mode However note that the display mode will not adjust automatically when set to maximum resolution 5Mi for 5M cameras or 2Mfull for 2M cameras or C Scan Specifically the display mode is adjusted as follows 5M cameras 1 3Mp 1 3Mi gt Shutter speed 1 3 second gt 5Mi lt Shutter speed 1 7 second lt 2M cameras 800 6 800 5 gt Shutter speed 1 7 second gt 2Mfull Shutter speed 1 15 second lt 35 SETU P VI Basic Operations 1 Preparations Before Photographing 1 4 3 Startup Condition Settings Power On Settings Display a menu and select the SETUP tag on it to enter the SETUP menu Select Power On Settings from the SETUP menu to display the Power On Settings screen shown below The following items can be set in the Power On Settings screen PowerOn Settings NetWork Settings File Settings Additional Return to Window PowerOn Settings CAM Custom No Sets custom mode to be Can OSD Cling 1 Bio used at startup Cross Line O OFF Display Custom No 1 OSD Position _ U Left Size O Big SXGA XGA LCD XGA LCD Color LCD ON LCD Back Light gg 5 Disp lay GA LCD Color Adjust R GAIN Oo 100 G GAIN OO 100 B GAIN _ _ 100 Mirror When you have finished
148. oes not respond even though it has had Save to Server assigned Even though the FTP send button is pressed the timer icon keeps running for about 1 minute and no images are sent An FTP error results when sent to the FTP server The Capture button is deactivated immediately after it is pressed Images cannot be saved to the FTP server even though the DS L1 is connected to it and no errors are displayed VIII Troubleshooting 5 Networking Corrective action When images are sent via FTP the DS L1 does not check for duplicate file names Although file names are also suffixed with a serial number when Auto setting is not used if the power is turned off once the serial numbers recycle and start from 1 again Therefore it is possible that one of the existing files will be overwritten Select Auto for File Name in File Settings of the SETUP menu and time information will be added to each file name helping to avoid overwriting files For FTP Dir too selection of Auto will help to avoid overwriting files because directories are separated by date If the DS L1 remains turned off for more than a week its internal timer will stop If the DS L1 is not used for an extended period of time reset the time of day The backup function recovers when the system is powered on e Check the network cable and hub connections e Select the Enable FTP Client check box in Network Settings of the SETUP menu This is because t
149. of the Disc Drive in Chapter VI 164 e When connected to a PC DS L1 is recognized not as a storage unit but as some other device such as a camera Unable to print with a PictBridge supporting printer e The printing icon for PictBridge Ss does not show e Unable to click the printing icon e A PRINTER ERROR message is displayed during printing VIII Troubleshooting 6 USB There may be a possibility that PictBridge supporting printer is selected in Additional Settings screen in the SETUP menu Select the selection mode for Mitsubishi DP900D printer using 1 4 5 Settings for Direct Print as a reference Confirm that PictBridge supporting printer is selected in the Additional Settings screen in the SETUP menu Confirm that a PictBridge supporting printer is connected to the USB D connector on DS L1 Confirm that the power for the PictBridge supporting printer is turned on In the case that it is still unable to click the printing icon even though the power is turned on close the menu turn on and off the power once more then display the menu again Check the paper and ink supplies as well as other conditions required for normal printing Restore the printer to the normal status by referring to the manuals for the printer you are using and then display the menu to perform the printing 1654 VIII Troubleshooting 7 Microscope Control from the DS L1 7 Microscope Control from the DS L1
150. oing so be sure not to touch the glass filter on the front of the CCD If you do touch the glass filter or if dirt cannot be removed from it contact your nearest Nikon representative and have them clean it e Do not use organic solvents e g alcohol ether or paint thinner to clean the coated plastic or printed parts Doing so could result in discoloration or in separation of the printing 2 Storage e Turn off the power when the system is not in use e If the system will not be used for an extended period of time unplug the power cord e Do not store the system in an environment that is hot humid dirty or dusty e Attach the C mount cap when storing the system 1505 VIII Troubleshooting 1 Power Supply VI Troubleshooting If you experience any problems check the troubleshooting list below If you are still not able to resolve the problem consult with your nearest Nikon representative 4 Power Supply Symptom The power does not come on The main unit is hot to the touch Corrective action Make sure the power cord is plugged firmly into an AC outlet at one end and the DS AC adapter and then the DC IN connector on the DS L1 main unit on the other Use the power cord supplied with the system Turn on the POWER switch The main unit temperature may increase when used in a hot or confined space If the high temperature is accompanied by a burnt smell or other offensive smells turn off the
151. omment to be mismatched use the freeze button to freeze the image before entering a comment 3 3 1 Free form Drawing The pen function allows you to draw a free form curve on the image NOTE You need a mouse to use this function 1 Press Pen The tool bar shown below will be displayed ROR Eie ear E Paste e 2 Press the red blue green black white or yellow color icon to determine the color of the pen 3 Click at the starting point and hold down the mouse button while you drag and the locus of the pointer will be drawn If you failed to draw use to erase the locus you ve now entered In this case what you ve drawn with the pen will be lost unless you have already pasted 4 After drawing press to fix what you ve drawn as valid data 5 Press to return to the menu in which you previously were Anything you ve drawn will be erased unless you have pasted Once the content is fixed as valid you cannot erase without erasing the entire overlay 68 VI Basic Operations TOO L 3 Measuring Two point Distance or Entering Information TOOL Menu 3 3 2 Using the Count Marker to Count the Number of Points The count marker is a convenient function because it displays numbers in the order in which markers have been attached Attach a marker to each point on the image and you can count the number of points easily NOTE You need a mouse to use this function 1 Press Marker The tool bar shown below
152. on 148 VI Basic Operations MICROSCOPE 6 Using the Microscope Control Objective lt Shows magnification and NA value of the objective gt Compatible only with Nikon 80i 90i microscopes that have motorized or intelligent nosepieces attached 1 The menu indicates the magnification and NA value of the current objective in the nosepiece column TI NOTE i Before using this function you must set up the objective information from a PC with the setup software For more information refer to the instruction manual for your microscope i Use microscope control menu 1 to change objectives Stage position Z axis lt Shows the address along vertical axis gt Ey Compatible only with the Nikon 90i microscope 1 The menu indicates the height of the current Z focus position i e position along the Z axis Fae NOTE Use microscope control menu 1 to adjust focus Ka Stage position X axis lt Shows address along lateral axis gt Compatible only with Nikon 90i microscopes connected to a motorized stage 1 The menu indicates the current lateral stage position position along the X axis K NOTE Use microscope control menu 1 to adjust the stage position laterally Be Stage position Y axis lt Shows the address along longitudinal axis gt Compatible only with Nikon 90i microscopes connected to a motorized stage 1 The menu indicates the current longitudinal stage position position along the Y axis NOT
153. on on the features of each DS camera head model refer to Chapter IX Technical Specifications DS 2MBW 1 1 8 inch black and white White 2 110 000 pixels e Indication of DS camera head model name In this manual the product names DS Camera Head DS 5M DS Camera Head DS 2Mv DS Camera Head DS 2MBW DS Cooled Camera Head DS 5Mc and DS Cooled Camera Head DS 2MBWc are abbreviated DS 5M DS 2Mv DS 2MBW DS 5MC and DS 2MBWc respectively The manual uses the term DS camera head when referring to all of these models e Illustrations in the manual In this manual illustrations of LCD monitor screens such as menu displays are based on the DS 5Mc Differences are indicated where displays vary depending on DS camera head model I Before Use 2 Components Components Check to see that all the items listed below are provided in the package If any items are missing contact your nearest Nikon representative immediately DS camera head C mount cap Camera cable 3 m E229586 f a aS CF DS Remote Controller DS RC Remote cable optional E120411 Direct Print License optional DirectPrint License code II C mount adapter optional Microscope Control License optional II Peripheral Equipment Peripheral Equipment 1 Analog RGB input display Although the DS L1 contains a 1 024 x 768 XGA 6 3 inc
154. on representative e Do not bend or twist the cables with undue force Doing so may damage the cables and result in fire e Do not place a cloth or paper on the DS L1 or DS AC adapter Doing so may cause heat to collect inside the equipment resulting in fire ZN CAUTION 1 Installation environment Do not use this equipment in locations subject to high temperatures high humidity vibration or excessive amounts of dust Doing so may result in fire or malfunction When used for an extended period of time the DS L1 may become hot Do not place the DS L1 on a surface that cannot withstand heat such as vinyl or plastic Always install the equipment with a surrounding clear area of 100 mm or more When using the fitting holes on the back of the equipment to install it always use metal fixtures that can support the weight of the DS L1 without directly attaching the equipment to the wall Leave a clearance of 100 mm or more from the wall surfaces and periphery 2 Always turn off power when setting up the equipment or when connecting disconnecting cables To prevent damage or malfunction always turn off the DS L1 s POWER switch when setting up the equipment or when connecting or disconnecting cables 3 Connect only the devices specified to the connectors To prevent damage to the equipment connect only the devices specified to the connectors on the DS L1 the DS camera head or the DS Remote Controller DS RC 4 Cautions on setup
155. on your PC you need to install it 1 From the Start menu of Windows select Settings Control Panel Network Services Add 2 Select Microsoft Peer Web Services from the Network Service list and click OK 3 Follow the instructions for installing Microsoft Peer Web Services You will be requested for a Windows NT CD ROM during this procedure 103 Settings VI Basic Operations 4 Using the DS L1 via a Network Adding a user Create a new user account 1 2 3 From the Start menu select Programs Administrative Tools User Manager Enter a user ID and a password To ensure that the same information has been set for External Server in the Network Settings of the DS L1 enter the user ID and the password using ten alphanumeric characters in lower or uppercase For Group set to Users limited user document saving allowed or above Specifying the home directory and enabling files to be written to it Enable image files to be written from the DS L1 to the PC by FTP 1 2 3 4 From the Start menu select Programs Microsoft Peer Web Services Internet Service Manager Select a PC name In the ensuing Properties dialog box select Service Properties and the Directory tab Choose a display directory and edit properties Here specify the home directory to which image files are to be transferred Also select the Write check box to enable writing
156. or the Password Enable the FTP server PC for anonymous access As in home directory settings select the Allow Anonymous Logon check box on the Security Account tab in the Preset FTP Sites properties window Be aware that if you select the Allow Only Anonymous Logon check box no one but an anonymous user can log on When specifying a directory in which to save image files separately for each of multiple DS Lis connected 1 2 Select Computer Management Local System Tools Local Users and Groups Users Double click the user you want to set and specify a local path for the home folder on the Profile tab The specified path should be below the home directory however Example If the home directory is C DSFTP the user home folder local path of the 3 DS L1 is C DSFTP DS1 and the FTP Dir on the DS L1 side is FTPIBOX then the FTP transfer directory is C DSFTP DS1 FTPIBOX Set local paths for the second and the third users DS L1s as C DSFTP DS2 and C DSFTP DS3 That way you can specify a separate directory for each user 107 N ETWO RK VI Basic Operations 4 Using the DS L1 via a Network 4 6 1 3 When Using Mac OS X Setup procedure 1 Setting up the FTP server Add FTP server functions 2 Adding a user entering the user ID and password Set the DS L1 as a log in user This setting must be made after logging in with the user ID of
157. orward direction or the left or bottom cursor button to select items in the reverse direction ZOOM button electronic zoom button Use this button to apply an electronic zoom The image zooms in or out at the magnification selected for E Zoom Step in Additional Settings of the SETUP menu FREEZE button freeze button This button allows you to switch the state of a photographed image between freeze and live CAPTURE button capture button Use this button to save an image This button can be assigned a save destination CF card FTP server or printer The image will then be saved to the destination selected for Capture Function in Additional Settings of the SETUP menu 1 1 2 Using the DS Remote Controller DS RC FREEZE CAPTURE FREEZE button CAPTURE button FREEZE and CAPTURE buttons These buttons serve the same function as the FREEZE and CAPTURE buttons on the front panel of the DS L1 a2 s VI Basic Operations SETU P 1 Preparations Before Photographing 1 1 3 Using a Mouse You can use a USB mouse with the DS L1 Be sure to use a recommended mouse Not every USB mouse is compatible with the DS L1 While most normal operations involve left clicking the mouse some may require right clicking For icons to set a value or select a mode left or right clicking the mouse button allows you to increment or decrement a value or select items in the forward or reverse direction e For an icon to increment or decrement a va
158. otographed data Recommended example DIC observation PH observation etc TONEG6 Provided for metal tissue Use this tone when you want to suppress unevenness in bright parts while photographing a high contrast image Recommended example Metal tissue or ceramics and other materials TONE7 Enhanced contrast Use this tone when you want to enhance the contrast during observation Recommended example Metal tissue or ceramics and other materials photographing documents with a lens etc 5 Adjusting the white balance Before photographing an image you need to adjust the white balance of the camera using a subject that has even whites 1 When using diascopic illumination for microphotography photograph a transparent part of the preparation When using episcopic illumination for microphotograph or when using a lens adjust the white balance with a white subject For fluorescent photography we recommend that the white balance be adjusted under normal light conditions before photographing an image m CAM ERA VI Basic Operations 2 Photographing Images 2 Next adjust the exposure It would be ideal if the amount of incident light can be adjusted in such a way that the shutter speed falls within a range of about 1 7 5 to 1 250 seconds To adjust the amount of incident light adjust the luminous energy of the light source or the iris diaphragm or use an ND filter 3 Press while in this state and the white balance will be auto
159. owing a large menu 76 3 4 6 VI Basic Operations TOO L 3 Measuring Two point Distance or Entering Information TOOL Menu Measuring circles You can measure the diameter and circumference of circles drawn on the screen and save circles and measurement results diameter and circumference as part of the overlay for the image 1 2 3 4 5 Press Measure The tool bar shown below will be displayed ZELKO unao fe Redo a Clear g Paste Press a color icon red blue green black white or yellow to set the color of the circle Select the C icon A circle of arbitrary size is displayed in the center of the image To move the circle click near the center and drag to where you wish the object to be located To resize the circle click near the circumference and drag to the desired size The diameter and circumference will be displayed in the vicinity of the center Each measurement result is assigned numbers 1 to 9 The number of circles drawn in the same color is limited to nine To change colors click another color icon so measurement results in that color will be assigned numbers separately from those in other colors To redraw a circle use or to cancel or redo your most recent action Up to 10 recent actions can be retrieved Use to erase all the content you ve now entered All lines drawn before confirmation with will be erased NOTE A To measure a new circle select the C icon once again
160. pad to enter the magnification of the objective Click the Relay Lens numeric value input box to display the numeric keypad Use the keypad to enter the magnification of the relay lens If you want to attach information to the calibration value you ve created click the Info input box and enter text for the information you want to attach This information will prove helpful because you can confirm Info when you call up the calibration value at a later time Check the Set number to confirm that the calibration number is correct then click to save it is disabled if no value is entered Press to return to the previous menu Any calibration values not saved are deleted NOTE i If the microscope connected to your system is the Nikon 80i 90i microscope with motorized or intelligent nosepiece and the calibration method you selected is using the magnification of the objective and relay lenses you do not need to enter the magnification of the objective If the camera head is connected to the rear port of the DIH you do not need to enter the magnification of the relay lens If you calibrated with one objective other connected objectives will be also calibrated automatically with their magnifications being converted i If you selected the using the magnification of the objective and relay lenses calibration method keep in mind that the calibration value may incorporate a certain amount of error attributable to optical errors of the objec
161. presents the CCD mode during image acquisition when you freeze save or print images The DS L1 will switch CCD modes during image acquisition if the source setting of saved images is a higher resolution than the display mode Note that switching CCD modes will result in a brief delay In certain cases you should avoid switching CCD modes for example when you expect to freeze images repeatedly To keep the DS L1 from switching CCD modes display images ina mode having resolution equal to or higher than the source setting The CCD mode will not switch if you view images saved at the lowest resolution 640 480 for 5M cameras or 400 300 for 2M cameras On the other hand the CCD mode will switch automatically if the display mode is set to C Scan If the quality of saved images is important choose the highest resolution for the source setting s5M for 5M cameras or s2M for 2M cameras 29 VI Basic Operations SETU P 1 Preparations Before Photographing Display mode Performance CAM menu EN Progressive Source CCD mode switching cameras 7 F BOER Features of display modes The various display modes offer different advantages as described below 5M cameras Cameras suitable for high resolution applications 5Mi An image area of 2560 1920 pixels is reduced for display at the monitor resolution 1280 960 SXGA or 1024 768 XGA The frame rate is 3 75 fps This display mode is useful when viewing images in great
162. r Safely remove USE Mass Storage Device DrivefT Safely remove USE Mass Storage Device DrivelH 3 Drives should be selected one at a time First select the media drive If the media drive can be removed the message shown below is displayed x I J Safe To Remove Hardware The USB Mass Storage Device device can now be safely removed From the system 4 To remove the CCD image drive follow step 3 5 Unplug the USB cable or turn off the DS L1 Plug in the USB cable and turn on the DS L1 It will be recognized by the PC again 150 5 VI Basic Operations MICROSCOPE 6 Using the Microscope Control 6 Using the Microscope Control 64 Overview If the DS L1 and the Nikon 80i 90i microscope are connected via USB the microscope can be controlled using the DS L1 menus The microscope status can also be displayed on DS L1 menus The items for which the microscope can be controlled and its status can be displayed this way depends on the specific microscope in use Please check the specifications of your microscope system before using the control features described here For more information refer to the Precautions regarding the menu display in Section 6 2 Menu Overview Before using this function you must purchase a license from Nikon For detailed information on obtaining a license please contact your nearest Nikon representative NOTE i If you do not have a license a license input menu appea
163. r minute Sets date and time Time o7 23 Power Save Timer No Operation Time 30 min 00 min Power Save off Sets power save timer Pointer speed tow fx Mid High Sets mouse speed E Zoom Step mr 140 w 200 f 280 w 400 100 j m wom E E E rA E coe lt Sets electronic zoom magnification Assigns CAPTURE External Out on x off button function Capt ure Funct ion x Save to Card Save to Server Print Front Panel amp Ex In x Mitsubishi CP900D PictBridge Printer Hag fix Brt _ 0 L _jo Hass S OFF relay 0 7 G J 0 USB DEV 3 6 _J co License _ comol 100 SAVE Default LCD Chart o ma ie _3 _4 5 _6 7 ee 9 220 0202 1717 040825 When you have finished setting each item press SAVE to save the data that has been set Or press Default to restore data to default values 28 VI Basic Operations SETU P 1 Preparations Before Photographing Date Time Enter a date and time NOTE Ifthe DS L1 remains turned off for extended periods its internal timer may slow down or stop When the DS L1 starts up it displays the date and time set in its timer flashes for about 8 seconds If you have shut off the DS L1 for one week or longer check the date and time shown on the DS L1 when you turn it back on Power Save Timer If you leave the DS L1 idle for a certain time it will automatically switch to power save mode
164. r V Microscope Adjustment Use an appropriate filter When using a phase contrast microscope or an interference microscope two luminous fluxes or multiple luminous fluxes insert the specified filter With a black and white image contrast is improved by inserting a green interference filter GIF Contrast in color images is enhanced by inserting a filter having a complementary color to that of the specimen gt See Chapter V Microscope Adjustment Adjust the ambient light around the microscope Make the room darker or place caps on the binocular eyepiece for example Check the contrast of the specimen itself Adjust the clock phase of the LCD monitor or projector gt See 1 4 4 Adjusting Monitor Output for an External Monitor in Chapter VI a Loo VIII Troubleshooting 3 Photographing and Saving 3 Photographing and Saving Symptom Captured images have low resolution and the overlay information cannot be read Photographing is mistimed even when freezing Camera modes change unintentionally Corrective action e Set the image quality in File Settings of the SETUP menu Reduce the JPEG Quality compression rate or select No compression BMP gt See 1 4 2 Image File Settings in Chapter VI Be sure to select a combination of camera mode and source that will not cause photographs to become mistimed i e that will enable satisfactory snapshot gt See 1 4 2 Ima
165. rate use to erase the current calibration value before saving it J1 8 VI Basic Operations 3 Measuring Two point Distance or Entering Information TOOL Menu Press to return to the previous menu Any calibration values will be erased unless they have been saved NOTE If you select E on the calibration screen calibration values are converted to inches or millimeters before saving Values entered in centimeters or millimeters are converted to inches while values entered in micrometers or nanometers are converted to millimeters lt When using the magnification of the objective and relay lenses gt 1 2 3 4 5 6 7 Select a number in which you want to save the calibration value from through s7 Drag the mouse pointer over one of numbers through s7 and the calibration information for that number will be displayed in the Info area for your confirmation NOTE If the microscope connected to your system is the Nikon 80i 90i microscope with motorized or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through s7 The value is selected automatically from through based on the hole number of the nosepiece you are currently using Press to bring up the calibration screen shown below Click the numeric value input box to display the numeric keypad Use the key
166. rd displays one of the following menus e F1i CAM menu e F2 VIEW menu e F3 REC menu e F4 TOOL menu e F6 MIC menu To change menus select your desired tag on the current menu with a cursor button and hit the Enter key on the keyboard to confirm 97 SETU P VI Basic Operations 1 Preparations Before Photographing DETE Initial Settings When using the DS L1 for the first time after purchasing you must make the following environment settings before photographing any image 1 Date and power save settings Set the unit clock and power save timer 2 Image file save related settings Set an image file format and compression rate the folder and file names in which to save the file and the source photographing mode Also make settings for entering camera information or comments and for saving a log file at the same time 3 Startup condition settings Set startup conditions for photography and display states To make these settings display a menu and select the SETUP tag to enter the SETUP menu The correct procedures are detailed below 1 4 1 Date and Power save Settings Additional Settings Display a menu and select the SETUP tag to enter the SETUP menu Select Additional from the SETUP menu to display the Additional Settings screen shown below PowerOn Sett ings NetWork Settings File Settings Additional Return to Window Additional Settings year month day Date 20 04 07 30 hou
167. re limited to the ones specified by the standard specifications of the printer you are using For details of the printer specification refer to the manuals came with the printer 64 VI Basic Operations TOO L 3 Measuring Two point Distance or Entering Information TOOL Menu 3 Measuring Two point Distance or Entering Information TOOL Menu 384 Outline of the TOOL Menu Display a menu and select the TOOL tag on it and the TOOL menu will be displayed In the TOOL menu the following functions are available to utilize the photographed image Note In overlay you can measure the distance between two points enter a comment or compare images laid on top of another You can place overlaid information on top of a photographed image before saving or printing the image however that the TOOL menu is subject to the following limitations A mouse is needed for almost all operations performed from the TOOL menu Although text can be entered from a keyboard you need a mouse to use this function The TOOL menu cannot be used in C Scan mode The TOOL menu is disabled during electronic zoom The contents you drew or entered from the TOOL menu are hidden when you turn on the electronic zoom The hidden contents can be redisplayed after you turn off the electronic zoom If the microscope connected to your system is the Nikon 80i 90i microscope with motorized or intelligent nosepiece the content drawn with the TOOL menu displa
168. re the menus displayed on DS L1 are explained for the case in which the Nikon 90i microscope incorporating DIH E and having all motor driven parts attached full spec is connected in a microscope system Keep in mind that if any of these parts do not exist or manual parts are attached menus for these parts will not be displayed Lav 5 M ICROSCO PE VI Basic Operations 6 Using the Microscope Control Menu configuration when the microscope connected has all motorized parts attached Select this check box when you want to connect the camera head to the front port of the DIH Snie Focus 1 1 Coarse fine selector Fine motion Coarse motion SAPE Escape The stage descends for specimen replacement F Autofocus Motorized stage ee ee 0 Coarse fine selector fFine motion Coarse motion F Fo rt an G 00 Returns the stage to the origin the stage can be moved lA y aF for easier specimen replacement Rapid transfer mode Y Stae DERE WMH Objective eE Shows magnification and NA value Shows detail information IP E E ee jE Selects the objective to use w Ew ag Optical zoom ga j Changes the zoom magnification of the rear port of F the DIH EJ ams Optical path POR oF F oy Binocular a Front port al EN lt aia B pa Rear port Changes and shows optical path il al Rear port when DIH M is connected for Info 30 70 illumination IE Closed
169. recognized as one removable disk by the PC When the DS L1 is connected to a PC two removable disks are displayed in My Computer on your PC s desktop iol x File Edit View Favorites Tools Help Back gt tj search GyFolders 3 G GS XxX A EE Address amp my Computer z 60 E314 Floppy 4 JLocal Disk C JLocal Disk D My Computer Local Disk E JLocal Disk F Removable Disk I 3 Compact Disc G Removable Disk Removable Disk H Removable Disk 1 Capacity 60 8 MB sh on Psgserv N S Psadata on Psgsery T I Used 22 6 MB x ie Panel i i C Free 38 2 MB 4 object s selected My Computer The drive names of the removable disks differ with each PC environment NOTE 1 2 3 4 In the following cases there might be a conflict between another connected device and the drive letter When media is inserted correctly into position the message Please insert media is displayed when you click the icon for the media drive A similar message is displayed when you click the icon for the CCD image drive Connecting the DS L1 will immediately disable all other USB devices connected to the PC In such a case change the drive letter assigned to the media drive or the CCD image drive The following shows how to make this change Select Start Control Panel Administrative Tools Computer Management Disk
170. reeze LED lights when the display is frozen CAPTURE Saves an image to the destination that you selected in Additional Settings The destination to which you have saved or printed is indicated by an LED CF CARD PRINT LAN III Names of Parts and Their Functions 1 DS Camera Control Unit DS L1i Left side of the DS L1 USB device port Connect a PC to this port with a USB cable to send and receive data to and from the PC Or connect a PictBridge supporting printer to perform the direct print H ESN a asn 12 V DC output This output is currently unused USB host port Use this port to connect a USB mouse USB keyboard USB printer CP900D or Nikon microscope 12 V DC input Use a dedicated AC adapter Network Tx indicator Lights when sending data to a LAN WOOL OL 7 TJ a Network Rx indicator Lights when receiving data from a LAN D LAN port Connect the DS L1 to a LAN with a LAN cable to exchange data with a PC or server through a LAN Right side of the DS L1 o p Stand arm l Extract the arm to use it a oe lt T n CF card slot 5 Insert a CF card into this slot to save or reproduce f Ati data to or from the card Make sure you re inserting the card in the correct J direction III Names of Parts and Their Functions 1 DS Camera Control Unit DS Li Rear of the DS L1 OC JT Io we RGB connector Use this connector to
171. rger e g 1 e When using diaphragm settings brighter than F2 8 you may observe slight light falloff around the periphery 8 Network connection cable You ll need a network connection cable to save image data on another server or to control the DS L1 from another computer over a network Use a cable for 100Base TX Category 5 or 6 To satisfy EMC standards use a shielded cable When connecting the DS L1 to a LAN use a straight cable When connecting the DS L1 directly to a PC use a crossed cable 9 USB cable You ll need a USB cable to save image data to a PC or to print directly to a printer via USB We recommend using a USB 2 0 compliant AB type cable for connections to a PC and an AB type cable for connection to a printer II Peripheral Equipment 10 Personal computer PC A PC is required for remote control of the DS L1 or to handle image data on other media A PC can be connected to the DS L1 via USB or over a network For connect a PC to a network we recommend the following configuration OS Windows 98 NT 2000 XP Macintosh OS 8 6 9 2 X Memory 64 MB or more Browser Windows Internet Explorer 5 5 6 0 Netscape 6 2 7 0 Macintosh Netscape 6 2 7 0 Although Nikon has confirmed that the DS L1 operates properly under the above conditions DS L1 operations cannot always be guaranteed since they are affected by OS or browser versions and by various software settings 11 Nikon 801 901 Microscope LV150A Indust
172. rial Microscope If you have a Nikon 80i 90i microscopes equipped with a digital imaging head you can purchase a license to control the microscope or display its status using the menus on DS L1 To connect your Nikon 80i 90i microscope to the DS L1 use the USB cable provided with the microscope For information on purchasing a license please contact the nearest Nikon office or distributor To connect the LV150A industrial microscope Nikon specifies the USB 232C cable This cable enables display of nosepiece status No license required III Names of Parts and Their Functions 1 DS Camera Control Unit DS L1i Names of Parts and Their Functions ao DS Camera Control Unit DS L1 Front of the DS L1 Internal LCD 1 024 x 768 dots 6 3 inch LCD display MENU button Press the MENU button to bring up a menu After a menu is displayed the MENU button can be used to confirm a selected menu command just like the ENTER button Cursor buttons Use one of the four cursor buttons to choose an item within a menu and the MENU button to confirm what you ve selected 200M FREEZE POWER switch This is a push switch Press it to turn on the power Press again to release it and turn off the power When the power is on the LED indicator lights and remains on Freeze LED FREEZE CF CARD o ARRE O O PRINT l LN O gt ZOOM Electronic zoom button FREEZE Freeze pause button The f
173. ring the IP address 192 168 1 2 Use semicolons to separate entries conest_ 8 Click OK Move the pointer 1 to the position of OK and left click on it Repeat this until the screen returns to the one from step 1 Oj N ETWO RK VI Basic Operations 4 Using the DS L1 via a Network 4 3 1 2 When Using Netscape 1 Start the PC Refer to your computer s user manual for details 2 Launch Netscape Refer to your computer s user manual or the Netscape help function for details 3 Select Edit Preferences Left click here to open the pull down menu and select Preferences from the menu Gut GIFY 4 a A 3 A Home Search Netscape Erini Security Shop Sop Ga What s Related Bopy CEE Paste Ginty Select AIl Ctrit A Eindin kasen Gtr End Agan CGtritG Search Internet Preferences 4 Select Advanced Proxies Lift click Advanced When a menu appears under Advanced left click on Proxies Category E Appearance Fonts Colors 3 Navigator MV Automatically load images Languages Applications Smart Browsing M Enable JavaScript H Mail amp Newsgroups V Enable JavaScript for Mail and News H Roaming Access H Composer H Offline eeAcdvanced ae Change preferences that affect the entire product MV Enable Java iV Enable style sheets I Send email address as
174. rint NOTE j The icon cannot be selected unless a printer is connected to the system F If a license has not been obtained the icon is displayed in black and white 2 3 2 Various Print Related Settings When CP900D printer is selected the following various settings can be chosen Choosing print mode The direct print function allows you to print in either normal or real 10 mode Choose print mode in Additional Settings of the SETUP menu Normal print mode Unless the Mag Fix check box in Additional Settings is selected images are printed in normal mode by default In this mode an image is enlarged or reduced as will be suitable for the printer s paper size before being printed Real 10 print mode If the Mag Fix check box in Additional Settings is selected images are printed in real 10 mode When printing in this mode always be sure to use the relay lens optional Use the x0 7 relay lens for 5M cameras and the x0 55 relay lens for 2M cameras The scaling factor in this mode is equal to 10 times the objective magnification For example if you are using a x40 objective the print scaling factor is x400 The size of the subject can be calculated backward from the printed size and the objective magnification Subject size subject size as printed objective magnification 10 Adjusting the printing condition Use Additional Settings of the SETUP menu to adjust the printing condition Bright Use it to adjust the brightne
175. rm the time of day at which image files have been updated follow the procedure described below 122 VI Basic Operations 5 Using the DS L1 when Connected to a PC by a USB Cable Confirming the updated date time of image files 1 Open My Computer and display image file icons Then select View Refresh to update the image file icons displayed io File Edit view Favorites Tools Help m 2 oper foes E x aA E wv Status Bar address 1 Explorer Ba Lo Large Icons led Small Icons tin 61760 i List DS L1 Details Thumbnails Select an it Arrange Icons See also Line Up Icons My Doc i My Network Customize This Folder My Comput S G0 To Refresh 2 Right click any image file icon of interest and select Properties from the ensuing menu The updated date and time will be displayed 10 File Edit View Favorites Tools Help Back Search UyFolders 4 LS OS x e EE fe Open DS LLIMG Open With Co WinZip IMG1260 ee Modified 3 1 2003 2 41 PM Cut Copy Size 344 KE Create Shortcut Attributes normal Delete Rename Properties 123 5 USB USB 5 3 5 VI Basic Operations 5 Using the DS L1 when Connected to a PC by a USB Cable Removing the DS L1 from the PC Because the DS L1 is connected via a USB interface it can be removed from the PC even if the PC remains powered on However to prevent data from being corrupted by removing th
176. rom a CF Card VIEW Menu in Chapter VI NOTE i Media formatted in other than NTFS may not be correctly formatted in FAT16 depending on the formats used If so reformat media in FAT12 FAT16 or FAT32 on your computer before use Format large media over 2 GB on your computer before use a i ie a U S B VI Basic Operations 5 Using the DS L1 when Connected to a PC by a USB Cable USB interface boards and hubs The DS L1 supports USB 2 0 If the DS L1 and the USB interface board are connected in one of the following ways data is transferred at a rate of 12 Mbps compliant with the USB 1 1 standard USB 1 1 interface board DS L1i USB 1 1 interface board USB 1 1 hub DS L1i USB 2 0 interface board USB 1 1 hub DS L1i If the DS L1 and the USB interface board are connected in one of the following ways data is transferred at a rate of 480 Mbps compliant with the USB 2 0 standard USB 2 0 interface board DS L1i USB 2 0 interface board USB 2 0 hub DS L1i Make sure that the USB 1 1 interface board and USB 1 1 hub used in the system are compliant with the USB 1 1 standard and that the USB 2 0 interface board and USB 2 0 hub used in the system are compliant with the USB 2 0 standard To avoid unexpected problems we recommend that the interface board and the DS L1 be connected in only one hub stage and not in cascaded hub stages NOTE When multiple units of DS L1 are connected to a Windows 2000 machine equipped with a USB 1 1 i
177. rs when you select the MIC tag j If you do not have a license the microscope status display microscopy setup menu MIC Info menu is not displayed T Before using microscope control function from DS L1 you must register the microscope status from the PC using the setup software Consult the instruction manual for your microscope 62 Menu Overview Left clicking to select displays the microscope control menu By default microscope control menu 1 is displayed Left clicking to select displays the microscope control menu 1 Left clicking to select displays the microscope control menu 2 When you open a menu while the microscope is connected to your system the menu displayed on the left side of the screen for default settings will have the MIC tag displayed on it Also a microscope status display microscopy setup menu MIC Info menu is displayed on the right side of the screen Selecting the MIC tag displays menus for controlling the microscope Switch between microscope control menu 1 and microscope control menu 2 by clicking either tab as necessary From these menus you can perform the following operations lt Microscope control menu 1 gt e Adjust focus e Move the stage e Change the objective e Change the optical zoom e Change the optical path e Change the shutter LoL MICROSCOPE VI Basic Operations 6 Using the Microscope Control e Change the filter e Control the lamp lt Micro
178. s 4 2 1 Items Needing Confirmation Items Needing Confirmation A LAN in some DS L1 operating environments may have been set up in a way exclusive to that environment so be sure to consult the network administrator in your organization before setting up a PC This is necessary to avoid causing problems with the LAN Ask your network administrator to confirm the following points e Whether FTP can be used in the LAN only if you wish to use the FTP function e Whether image files can be transferred across in the LAN e How IP addresses are managed if you wish to use a fixed IP address whether its use is permitted or if you wish to have IP addresses etc automatically acquired whether a DHCP server is available NOTE When a fixed IP address is acquired a subnet mask and a gateway address are also acquired Note however that when you apply for an IP address you may be requested to supply the MAC address of your product The MAC address is shown in the lower right corner of the Network Settings screen of the SETUP menu In automatic IP address acquisition by DHCP the assigned IP addresses change dynamically Accordingly if you are using a web browser to access the DS L1 from a PC we recommend setting a fixed IP address for the DS L1 Confirming the PC to be network connected e OS of the PC used for a Web browser and browser versions There may be slight differences in Web browser functions depending on the OS of the PC and t
179. s extremely high image resolution the slightest vibration can affect image quality To insulate the microscope from vibration set it on a solid stable surface on a solid floor Using an anti vibration table compatible with the microscope will further reduce the effects of vibration While actual microphotography is in progress be careful to avoid touching the microscope or the desk or stand on which it is positioned Mpegs SETU P VI Basic Operations 1 Preparations Before Photographing Basic Operations Preface to Operating Instructions The DS L1 Ver 3 00 or later supports the five camera heads listed below If you change camera heads the DS L1 control unit will automatically switch modes in response DS 5M 5 megapixel CCD color non cooled DS 5Mc 5 megapixel CCD color cooled DS 2Mv 2 megapixel CCD color non cooled DS 2MBW 2 megapixel CCD monochrome non cooled DS 2MBWc 2 megapixel CCD monochrome cooled For detailed information on these cameras refer to Chapter IX Technical Specifications The following terms are used in this manual to refer to different cameras 5M cameras gt DS 5M and DS 5Mc 2M cameras gt DS 2Mv DS 2MBW and DS 2MBWc Cooled cameras gt DS 5Mc and DS 2MBWc Non cooled cameras gt DS 5M DS 2Mv and DS 2MBW Monochrome cameras gt DS 2MBW and DS 2MBWc Example screenshots are based on the DS 5M When using 2M cameras keep in mind that size specifications suc
180. s that have partial highlight reflections e Recommended example Fluorescent photography stereoscopic microscope observation metal photomicrography F_AE Choose F_AE when the subject is dark image updates go slow and you have difficulty adjusting the focus Increase the camera gain to prevent the exposure time from increasing e In this exposure mode noise will stand out because the gain is high but when using exposure start button the image is photographed after automatically increasing the exposure time and decreasing the gain e Even when F_AE mode is selected if the subject is relatively bright the original combination of display mode and source will be followed e When using MI freeze button the noisy image will be paused 49 VI Basic Operations 2 Photographing Images NOTE Adjusting the focus of a dark subject can also be performed by pressing the focus button When the button is right clicked the camera gain is increased to adjust the exposure automatically so that the exposure time will not be too long Then the live image is displayed while keeping the exposure status When right clicking on the O the response at focusing NR OFF status is set Left click on the KFycfocus button to access manual mode without adjusting the exposure focus button to increase 3 Choosing photometry mode There are two photometry modes b Average photometry VH Peak hold photometry
181. scale and the scale currently displayed is deleted For detailed information about the overlay data refer to the description of the TOOL menu ij When you change the objective the XY scale and the scale currently displayed also are changed 141 MICROSCOPE VI Basic Operations 6 Using the Microscope Control vai Optical zoom lt Changes optical zooms gt m lt gt Compatible only with microscope incorporating the DIH E 1 2 3 ae Connect the camera head to the rear port of the DIH Confirm that the check box _ F Po i that will be selected marked by a check mark when the camera head is connected to the front port of the DIH is unselected now If you want to reduce the magnification of the optical zoom below its current magnification select the icon in the optical zoom column Ee Conversely if you want to increase the magnification select the icon to change the magnification NOTE t For the microscopes incorporating the DIH M only the magnification is displayed The magnification changes in increments of x0 1 and can be changed in the range x0 8 to x2 0 i The current magnification is displayed on the microscope status display microscopy setup menu MIC Info menu j When you change the optical zoom the episcopic illumination field diaphragm is also changed accordingly For more information refer to the instruction manual for your microscope When you change the optical zoo
182. scope control menu 2 gt e Change the condenser e Adjust the diascopic illumination aperture diaphragm e Adjust the diascopic illumination field diaphragm e Adjust the episcopic illumination field diaphragm e Adjust the transmission factor of the ND filter e Change the analyzer lt Microscope status display microscopy setup menu gt MIC Info menu e Show the name of the objective e Show the position of the stage in height direction e Show the position of the stage in longitudinal and lateral directions e Show the magnification of the optical zoom e Show the name of the filter e Set up microscopy and show the current microscopy lt Precautions regarding menu display gt Depending on the type of microscope connected to your system not all items of the microscope control menus and microscope status display menus can be displayed The items that can be displayed on the DS L1 menu are limited to those for parts whose status can be detected electrically among the various parts of your microscope Keep in mind that the items displayed on the DS L1 menu do not include those for parts whose status cannot be detected electrically Refer to the table below for the relationship between the microscope system and menu display 152 5 VI Basic Operations MICROSCOPE 6 Using the Microscope Control 1 When the Nikon 80i microscope incorporating DIH M is connected arts Menudisplayed O O Manual nosepiece No menu displayed 2 When the
183. scopic illumination field diaphragm Control status display menu displayed Episcopic illumination field diaphragm No menu displayed With motorized ND filter Control status display menu displayed ND filter Without motorized ND filter No menu displayed a Eois a MICROSCOPE VI Basic Operations 6 Using the Microscope Control When the Nikon 90i microscope incorporating DIH E is connected a a Nosepiece Motorized nosepiece Control status display menu displayed Shutter Control status display menu displayed Filter cube Control status display menu displayed Motorized universal Control status display menu displayed Universal condenser condenser Non motorized universal l No menu displayed condenser Diascopic illumination With motorized diaphragm Control status display menu displayed aperture Without motorized i No menu displayed diaphragm diaphragm Diascopic illumination field diaphragm Control status display menu displayed Episcopic illumination field diaphragm Control status display menu displayed With motorized ND filter Control status display menu displayed ND filter Without motorized ND filter No menu displayed 130 gt VI Basic Operations MICROSCOPE 6 Using the Microscope Control 6 2 1 Microscope Control Menu 1 Select the tab 1 of the microscope control menu to display microscope control menu 1 In microscope control menu 1 you can control the motorized units of the microscope used often He
184. screen it is possible that the browser will no longer be updated In such a case wait for a while or temporarily close the browser and reopen it Sometimes the window may be closed while downloading an image file To avoid this problem we recommend installing SP1 Service Pack 1 if you are using Internet Explorer 5 5 When you open an image file in the DS L1 directly from a PC ona network it is possible that the original window will be closed In that case first download a file to the PC before opening it When using Mac OS to download a BMP file select Save a File to Disk to save the file first and then open it in your application You cannot directly open a downloaded file Pickup JPEG Click the image size you want to download and the latest image in that size will be displayed in a new window 1 2 When you click any image size to select Save it to disk a screen is displayed for you to select the folder in the PC that you wish to save to When you right click on the displayed image the image can be downloaded into the PC in JPEG format If you select Save As in the displayed menu the screen shown below will be displayed Saven amp Nkn O H El E C NikonG L Nikon10 C Nikon C Nikon2 C Nikong L Nikon3 C Nikon L Nikon4 L Nikon5 Filename oupes SS Save as type JPEG jpa Cancel Select the folder in the PC where you want to save the image file Enter a file name
185. selected number By selecting multiple numbers you can display concentric circles with different radiuses 7 Crosshairs is an option that allows you to select the line type of crosshairs Select the Normal check box and the crosshairs will become a solid line When you select the Dotted check box the crosshairs is drawn with broken lines 78 VI Basic Operations TOO L 3 Measuring Two point Distance or Entering Information TOOL Menu 8 Center Cross is an option that allows you to fill the center part of the crosshairs Select the Yes check box to fill the center part of the crosshairs If you select the No check box the center part of the crosshairs will not be filled 9 Press to move the center position of the crosshairs back to the center of the screen 10 Press to save the crosshairs settings The display position size and color and display mode you ve set in 2 to 8 above are saved to internal memory NOTE Crosshairs settings once saved remain unchanged until you execute again When you display crosshairs for the first time after starting the DS L1 the crosshairs will be displayed with the settings you last saved You must save any changes to the crosshairs settings otherwise they will not appear the next time you start the DS L1 11 Press to return to the menu in which you previously were 12 To hide the displayed crosshairs press again Normally crosshairs are displ
186. setting Seven modes Tool functions Two point distance measurement count marking function text input function pen function superimpose function scaling function crossline function Other functions Image freeze electronic zoom interval photographing two split screens CF card thumbnail display CF card reproduction Recorded image Recorded image file JPEG compliant BMP 24 bit format JPEG compression Fine Normal Basic Draft mode 167 Interface CF card USB1 1 host interface USB2 0 device interface Direct print Network General External dimensions Weight Rated input voltage Power consumption Structure of fixed part Acoustic Noise IX Technical Specifications 1 DS Camera Control Unit DS L1 Type I Type II USB mouse USB keyboard USB printer CP900D connectable Nikon 80i 90i microscope LV150A industrial microscope Mass storage class supported PictBridge supporting printer connectable CP900D made by Mitsubishi Electric or a PictBridge supporting printer connectable IEEE802 3 compliant 1OBASE T 100BASE TX DHCP supported HTTP server Telnet server FTP server FTP client 203 W x 202 H x 73 D mm not including protrusions 1 3 kg 12 VDC 5 Approx 138 VA VESA 75mm standard 75 mm pitch M4 x 7 fixing screw holes 4 locations UL1678 suited stand and mount Less than 70 dB Maschinenlarminformations Verordnung 3 GPSGV 06 01 2004 Der h chste Schalldruckpegel betr
187. splayed at the bottom of the menu When using the E exposure start button histograms are automatically displayed lis Liss UIE This histogram indicates the distribution of brightness of each R G and B color and can be used for fine adjustment of the exposure level e The horizontal axis indicates the color brightness increasingly darker toward the left and brighter toward the right e The vertical axis indicates the frequency of occurrence which is normalized by the maximum value e The average histogram values of each color are shown in numeric form at the right edge and shown by levels at the bottom How to read and set exposure levels If the brightness distribution is concentrated on the right edge of the histogram it means that the highlighted parts of the screen are saturated Conversely if the brightness distribution concentrates on the left edge of the histogram it means that the low lighted parts of the screen are blacked out Although it is ideal if no parts on the screen are saturated or blacked out exposure may have to be adjusted depending on the subject If an area of interest on the screen is highlighted adjust the exposure so that the distribution will be shifted toward the left If an area of interest is low lighted adjust the exposure so that the distribution will be shifted toward the right Knowing the exact level of exposure that is needed while you adjust the exposure will help to prevent insuffici
188. ss of a print Use the left button to make it darker or the right button to make it brighter R_GAIN G_GAIN B_GAIN You can adjust the gain of each R G and B color individually CMP 90 110 Use it to adjust the scaling factor during real 10 Mag fix mode Errors in optical magnification can be adjusted Try printing a subject whose size is known and measure its printed size If an error is found correct Repeat a sequence of correction printing and measurement several times until an exact value is obtained 63 CAM ERA VI Basic Operations 2 Photographing Images Selecting a source Use File Settings of the SETUP menu to select a source Choose the s5M for 5M cameras or s2M for 2M cameras source to obtain satisfactory picture quality The features of each source will be the same as when saving an image Refer to 1 4 2 Image File Settings File Settings in Chapter VI for details on how to set the file format image size compression rate and the source Checking the print range In real 10 mode the image is enlarged by multiplying it by an appropriate scaling factor when printed with edges of the image truncated Use the REC menu to check the print range Press Area and a printing range will be displayed Checking print information Information on the date time scale and comments are printed at the bottom of the printed image Scale During normal printing the scale is displayed in pixel
189. ss you first perform calibration or call up the saved calibration value i Because this is a simple function for measurement its accuracy is not high Press Measure The tool bar shown below will be displayed Select the Fa icon ZELKO Undo fe Reso 7 Clear g Paste S Press the red blue green black white or yellow color icon to determine the color of the measurement line nay ic 3 4 5 VI Basic Operations 3 Measuring Two point Distance or Entering Information TOOL Menu Draw a measurement line Click the mouse button at the start point of the object to be measured and hold down the mouse button while you drag and release the mouse button at the end point to draw a line measurement line The length of the measurement line will be displayed in the vicinity of it Each measurement line is assigned numbers 1 to 9 The number of measurement lines drawn in the same color is limited to nine To change colors click another color icon so that measurement lines drawn in that color will be assigned numbers separately from those in other colors To redraw a line use or to cancel or redo your most recent action Up to 10 recent actions can be retrieved Use to erase all the content you ve now entered All lines drawn before confirmation with will be erased NOTE If you draw a measurement line in the middle of or Redo recent measurement lines drawn from that point in time cannot be canceled To sav
190. st the white balance 6 Adjust the picture quality 7 Confirm exposure information 8 Save the image 9 Save call photographing conditions _A7 CAM ERA VI Basic Operations 2 Photographing Images 1 Choosing display mode Display mode here refers to one that is used to determine how an image is displayed on the screen Feeling or features of camera operation vary with a combination of display mode and the source you use during photographing Refer to Outline Guide to Source Photographing Mode Settings in 1 4 2 Image File Settings File Settings Chapter VI Sample settings that suit different uses are described below Try these settings before determining your own settings e Fine adjusting the focus for a subject consisting of minute patterns 1 3Mp 5Mi or 2Mfull Ex Semiconductor pattern low magnification photography of cells and tissue fluorescent photography landscape photography with a lens e Moving the subject while observing gt 1 3Mp 2Mfull or 800 5 Ex Semiconductor pattern stereoscopic microscope observation cell diagnosis general photography with a lens e Focusing on a dark subject gt 1 3Mi 800 6 Ex Position or focus adjustment during fluorescent photography nighttime photography with a lens e Photographing a dark subject in the shortest time possible gt 1 3Mi 800 6 EX Fluorescent photography nighttime photography with a lens e Photographing a bright subject wi
191. store the set values in memory are given in the description of the Advn menu Industrial specimen scene modes Wafer IC chip Wafer or IC chip Metal Ceramic Bright parts of a metal specimen are expressed as white and dark parts are expressed as transparent This mode can also be used for ceramic and plastic specimens Circuit Board This mode is suitable for photographing mounting defects on a part mounted circuit board Clipping of highlights in component leads and solder joints are suppressed allowing for defects to be easily detected This mode is also suitable for high contrast subjects such as gears and other metal components Biological specimen scene modes DF FL Dark field photography fluorescent photography Use this mode to photograph a dark subject The camera operability for position or focus adjustment is improved by increasing the camera s sensitivity High resolution images may be obtained from the photographed data BF Bright field photography This mode is provided for general dyed specimens DIC PH Contrast is enhanced for differential interference and phase contrast photography purposes 42 VI Basic Operations CAM ERA 2 Photographing Images Custom modes Custom mode is the mode in which the set values for individual subjects and objectives and those of scene modes are saved Pressing one of the buttons through calls up the saved content of the selected custom mo
192. structions indicated by these symbols Symbol Meanin AN WARNING Disregarding instructions indicated by this symbol may result in death or serious injury AN CAUTION _ Disregarding instructions indicated by this symbol may result in injury or property damage Trademarks Microsoft Windows and Internet Explorer are registered trademarks of Microsoft Corporation in the United States or other countries Macintosh and MacOS are registered trademarks of Apple Computer inc in the United States or other countries Netscape Navigator is a registered trademark of Netscape Communications Corporation The PictBridge logo mark is a trademark Products and brand names are trademarks or registered trademarks of their respective companies The TM and marks are not used to identify registered trademarks and trademarks in these instructions WARNING 1 Using the equipment This equipment should be used for photographic purposes only Do not attempt to use it for any other purpose 2 Do not disassemble or repair the equipment The DS L1 and DS AC adapter contain high voltage power supplies Never attempt to disassemble or repair the equipment by yourself doing so may result in electric shock or equipment failure Never attempt to disassemble any part of the equipment unless instructed to do so in this manual If you notice any problems with the equipment turn off the power and contact your nearest Nikon representative 3 DS A
193. sure start button The camera will automatically adjust the exposure and photograph an image with an s5M source After the exposure the image is automatically frozen 44 VI Basic Operations CAM ERA 2 Photographing Images NOTE If the exposure time exceeds 6 seconds is displayed By right clicking the mouse photographing is stopped Confirm the image and if satisfactory save the image Use the E capt button or the REC menu or the shortcut menu to save the image If the brightness needs adjustment change the exposure condition by using the C_gn camera gain adjusting button and the shutter speed adjusting button then photograph again with the exposure start button To return from this status to the live image press focus button To get out of the DF FL scene mode select another scene mode or the custom mode NOTE When connecting DS L1 with the Nikon 80i microscope equipped with DIH E or Nikon 90i microscope via USB the shutter for the episcopic illumination will automatically open or close corresponding to the DF DL scene mode operation This function is enabled only when observation method of the microscope on DS L1 is set for FL fluorescent microscopy For the setting for each observation method refer to 6 2 3 Microscope Status Display Microscopy Setup Menu MIC Info Menu in Chapter VI With this function the shutter opens by entering the DF FL scene mode in case the shutter has bee
194. switched to the one that is attached to the nosepiece number you ve selected 2 After switching objectives confirm that the selected number icon is marked with a red border and that the nosepiece number the objective magnification and NA value are displayed NOTE Before this function can be used you must first set up objective information from a PC using the setup software For more information consult the instruction manual for your microscope If you are using an intelligent nosepiece only the objective magnification and NA value are displayed Selecting Mapa icon shows detailed information on the objective being used Select the X mark on the detailed information display screen to turn the detailed information display screen off a Right clicking on the icon representing a nosepiece number shows the magnifications and NA values of all registered objectives You also can select the desired objective from within this display to change objectives i The magnifications and NA values of objective in use are also displayed on the microscope status display microscopy setup menu MIC Info menu When you change the objective the diascopic illumination aperture diaphragm diascopic illumination field diaphragm condenser Focus and ND filter also are changed accordingly For more information refer to the instruction manual for your microscope i When you change the objective all the overlay data excluding the XY
195. t a format gt See 1 2 Notes on Using a CF Card in Chapter VI 160 VIII Troubleshooting 5 Networking 5 Networking Refer to 4 2 Network Settings in Chapter VI for details about network settings Symptom Corrective action Cannot connect the Follow the sequence shown below to check network settings system to a network gt See 4 2 Network Settings in Chapter VI Check network cable connections cables and hub Check the set values DS L1 IP address and PC network settings J Check the presence in a LAN ping from the PC J Check browser settings Check FTP settings Checking network cable connections lt Checking types of cables gt e Check that the twisted pair cables for L1OBASE T or 100BASE TX are used e If the DS L1 is connected to a LAN via a hub check that straight cables are used e If the DS L1 is connected directly to a PC without going through a hub check that crossed cables are used lt Checking hub connections gt See the instruction manual supplied with the hub for details and follow the description written in it e Do not connect the DS L1 to the Uplink Be aware that Uplink on some hubs is indicated as MDI Uplink 1 x 1 1 x or 8 8 x Also if a hub has cross and straight ports caution must be exercised e There is a limited number of useful ports on a hub Be careful when connecting to an empty port because doing so could
196. t to make each entry consist of three digits as you enter Click the input box and enter an address value If you missed entering any address value hold down on the screen keyboard while you skip to the last entry of the address then enter the correct value again When you finished setting all press to save what you ve set The server with the number you last saved is selected when the DS L1 starts up L10 VI Basic Operations N ETWO RK 4 Using the DS L1 via a Network Connection test Ping test To test the connection state of the external server execute on the Network Settings screen This function is used to inspect whether the specified server exists in the LAN This connection test is performed on the server that is shown in the IP Address column of External Server However it is necessary as a premise that the IP Address has been saved by pressing SAVE When a response is received from the server a message OK Complete is displayed Ping may not always be responded to for reasons of security functions such as packet filter firewall or security gateway Ping test may be reexecuted after disabling the security function of the server In that case restart the DS L1 first Also even when Ping was not responded to there is no problem with the server connection only if the server can be logged in in the login test described below Login test To test logging in to the server execute Login Test lon the Ne
197. table NOTE The screen shots shown in this section may differ depending on which browser software or version is used Regarding the use of a browser e Depending on the PC environment and the browser version the WEB screens may behave differently or suffer some operational restrictions e When moving from ROOM1 or ROOM2 to another screen click the tab located at the upper right corner of the screen Click the Reload tab to redisplay Do not use the Back button on your browser If the Back button is used the display may not be updated In that case select View Refresh on your browser 94 VI Basic Operations N ETWO RK 4 Using the DS L1 via a Network Opening a WEB screen 1 Launch either Internet Explorer or Netscape Communicator on your computer 2 Enter the IP address of the DS L1 in the URL address field Example http 192 168 10 2 The Entrance screen of the DS L1 will appear 3 Click either ROOM1 or ROOM2 Please Enter the Room Click either ROOM1 or Image Window with Cotrol Pad amp Streaming ROOM2 CF Card Access ROOM1 and ROOM2 are explained in more detail in the subsequent pages 95 N ETWO RK VI Basic Operations 4 Using the DS L1 via a Network Using ROOM1 ROOM1 allows you to observe the image that is being shot by the DS L1 at specified intervals while at the same time capturing images onto the PC or displaying a stream window You also can control the DS L1 main unit
198. th priority placed on focusing C Scan 2 Choosing exposure mode Choose your desired exposure mode from PROG S_AE MANU or F_AE Normally PROG should be selected Sample settings for different uses are described below Try these settings before determining your own settings e Photographing a moving subject gt S_AE combination with 1 3Mp 2Mfull or 800 5 display mode e Eliminating fluctuations of exposure for moving subjects or when moving a subject while observing MANU or use AE_L in PROG e Photographing under the same conditions at all times MANU Features of exposure modes and instructions on use PROG Automatic exposure Choose PROG for general photography The DS L1 automatically determines exposure based on a combination of the shutter speed and camera sensitivity In this case the electronic shutter is automatically adjusted in the following range For display modes other than C Scan 1 s to 1 1000 s For display mode C Scan 1 15 s to 1 1000 s e If the image is excessively bri ght adjust the compensation icon CMP to the negative side Conversely if the image is excessively dark adjust the compensation icon CMP to the positive side 48 VI Basic Operations CAM ERA 2 Photographing Images e If fluctuations in exposure caused by movement of the subject presents a problem use to temporarily hold the exposure value During that tim
199. the DS L1 via a Network Internal Server Set the IP address subnet mask user ID and password of the DS L1 Refer to Settings for improved security described below for details on how to set a password e When automatically acquiring an IP address D H C P Auto get an IP IP Address Automatically set after restarting Subnet Mask Automatically set after restarting NOTE The DS L1 takes some time after it restarts before the addresses are displayed e When setting a fixed IP address D H C P _ Auto get an IP IP Address lt Input example Subnet Mask 255 lt Input example If any entry in the IP address consists of two digits or one digit add O or OO at the beginning so that entry will consist of three digits Click the input box and enter an address value If you missed entering any address value hold down on the screen keyboard while you skip to the last entry of the address then reenter the correct value Once you have finished with all the settings press SAVE and then restart the DS L1 To revert to the initially set value press Default and then SAVE and restart the DS L1 Settings for improved security To improve security make the following settings on the Network Settings screen Be aware that the transferred images and information are not encrypted Limiting logins by User ID and Password settings In the Internal Server set a user ID and a password User ID Input up to 1
200. the menu 2 JO TOO L VI Basic Operations 3 Measuring Two point Distance or Entering Information TOOL Menu 3 5 2 Using a Scale to Measure the Subject Size 10m You can display a scale on the screen and use it to measure an approximate size of the subject 1 Select the calibration value you want to use from through s7 2 Press Scale and a scale matched to the selected calibration value will be displayed 3 Press again to erase the displayed scale A scale normally is displayed on only the screen and is not drawn in the saved or printed image If you select the Scale check box on the REC menu two scales one vertical and one horizontal will be drawn in the lower right part of the saved or printed image NOTE x Depending on the calibration value such as when the screen full length is calibrated as being equal to 1 mm the system may not be able to display values In such a case the value is displayed as and you must change the unit of the calibration value from 1 mm to 1000 um etc a Normally values with these functions are labeled with the units nm um mm or cm However if calibration values are selected after inch mil conversion they are labeled mil and an E is indicated representing inch conversion z If you perform an electronic zoom while using this function the screen display will reflect the zoom magnification If you change the optical zoom of the di
201. ther half of the screen If you are using a mouse you can scroll the display up or down on either screen 1 Select on the VIEW menu Approximately half of the middle part of the screen displayed before being split moves to the left to become a still or frozen image Any part of the screen that has disappeared as a result of this operation can be made to reappear by scrolling with a mouse Move the mouse pointer to the boundary and it will change shape to become a cross hair pointer Now click the mouse button and the screen will scroll until the hidden part appears 2 To quit dual window display press SWIN on the VIEW menu NOTE Although the image can be saved while being displayed in dual windows be aware that you cannot save an image size of 2560 1920 If you select an image size of 2560 1920 it will be automatically reduced to 1280 960 for 5M cameras 62 VI Basic Operations CAM ERA 2 Photographing Images 23 Outputting Images Directly to a Printer 2 3 1 Printing an Image When operating from a shortcut menu or the REC menu use the print icon to print When selecting CP900D printer or a PictBridge supporting printer in the Additional Settings screen of the SETUP menu window a print icon either Mage or respectively is displayed When operating from the main unit panel switch or the DS Remote Controller DS CR you must choose Print for Capture Function in Additional Settings of the SETUP menu before you can p
202. ting printer on This also makes printing by the PictBridge supporting printer possible When this mode is selected the function to capture the image on the PC by connecting the PC to the USB D connector on DS L1 is disabled Also even if a CP900D printer is connected to the USB H connector printing by the printer is not possible PictBridge Standard PictBridge is the standard which allows to print the images photographed by a digital camera directly without a PC and the interconnection between a digital camera and a printer is supported by manufacturers of respective products For details refer to the PictBridge official website http www cipa jp pictbridge 1 4 6 Settings for Connecting a Microscope To control the microscope or display the microscope status you must input your microscope control license code Otherwise it will not function NOTE For information on purchasing a microscope control license please contact the nearest Nikon office or distributor Follow the procedure given below to set up the microscope control license 1 Display the menu and select the MIC tag 2 If the microscope control license has not been acquired yet a microscope control license input window is displayed 3 Enter your license code in the input window and press Ent This completes setup You do not need to restart after setup NOTE Ifa Code Error message is displayed in 3 above check your license code and reenter 3
203. tive With the Nikon 80i 90i microscope connected errors may range up to 10 ron 3 4 2 VI Basic Operations TOO L 3 Measuring Two point Distance or Entering Information TOOL Menu Calling the Calibration Value To use one of the registered calibration values to perform measurement you must call up the calibration value following the procedure described below before you can use it 1 3 4 3 NOTE Be aware that if you call up a calibration value for any image all of the overlay information for that image will be erased Select the calibration value you want to use from through s7 Drag the mouse pointer over one of the numbers through s7 and the calibration information for that number will be displayed in the Info area for your confirmation NOTE If the microscope connected to your system is the Nikon 80i 90i microscope with motorized or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting through s7 Calibration values are automatically changed when you change the objective Measuring the Distance between Two Points 1 10m You can measure the distance between two specified points on an image The measurement line you used for this measurement can be saved to the overlay for that image 1 2 NOTE i You need a mouse to use this function i This function can be used unle
204. tive magnification in a log may facilitate future confirmation You can write up to 100 characters to a log when saving an image to a card or server or up to 32 characters when outputting to a printer Photographed data including Nikon 80i 90i microscope data during photography is also contained in a log e When you save an image in BMP format the log is saved to a file named after the image with the extension txt added e When you save an image in JPEG format it is saved to a file named after the image with the extension txt added Further it is embedded in the image file as Exif information e When you output an image directly to a printer the log is printed as a comment NOTE You can attach a log when saving an image even if you didn t select Log during initial settings Select the Append check box for Log on the REC menu and enter a comment in the text box Photographing information and your comments will be saved in text format when you save the image File Type Set a file format an image size and a compression rate TYPE Select JPEG or BMP as the format in which you want to save a file SOURCE Select the source type based on photography mode This setting applies when freezing images saving image files or printing images directly Use this setting to ensure the image quality level for saved images You can also use this setting to avoid switching CCD modes when freezing images For 5M cameras c
205. tment for the external LCD monitor XGA LCD OFF Internal monitor is OFF and external monitor XGA output is ON Color adjustment for an external LCD monitor XGA LCD ON Internal monitor is ON and external monitor XGA output is ON Color adjustment for an external LCD monitor LCD Back Light Adjust the brightness of the internal monitor Display VGA LCD Color Adjustment Adjust the color balance of the internal monitor Use R_GAIN G_GAIN and B_GAIN to adjust each of the R G and B colors The color balance of external monitor output also changes when you adjust the color balance of the internal monitor Mirror Normal H V HV Only when 5M cameras are connected You can flip the image horizontally or vertically or rotate the image 180 degrees Set this function according to the orientation of the camera when attached to a microscope NOTE Mirroring is not available when 2M cameras are connected 1 4 4 Adjusting Monitor Output for an External Monitor If the DS L1 is connected to an external monitor you must adjust the monitor output on the Power On Settings screen Monitor output is initially set to 1 024 x 768 resolution 60 Hz XGA If observation with a higher definition display is required when photographing an image we recommend using 1 280 x 1 024 resolution 60 Hz SXGA output NOTE If you select SXGA output images cannot be displayed on the DS L1 s internal display If an external monitor is connected to t
206. to a LAN you need to enable it for network operation and set its own address To make network settings on the DS L1 select Network Settings from the SETUP menu to call up the Network Settings screen shown below PowerQn Settings NetWork Settings File Settings Additional Return to Window NetWork Settings Enable NetWork Read Only FIP WER disable Write Control Enables disables network operation BGP C duto get an IP Internal Server 1P address 192 Sets the IP address of the Sublet Mask 255 DS L1 User ID anonymous max 10 char Pass Word none max 10 char Enable FIP Client o IP authority External Server 1P address Server No User ID max 10 char Pass Word max 10 char cate vey 192 168 SAVE Default skip LO LiJ 2 L3 L415 e alb LA Li e A Lb JL ILkK JO JLo on Lo Le LaJLe bsJLt le by e fly Iz MAC Address 00 90 Enable Network To use a network always select this check box Read Only FTP WEB disable Write Control This is to disable writing to the internal files of the DS L1 when it is operated using a WEB browser or FTP command When this check box is selected you cannot write to the internal files Unselect this check box to control the DS L1 and write to internal files via a network connection If you only want to get images from the DS L1 leave this check box selected 87 N ETWO RK VI Basic Operations 4 Using
207. twork Settings screen A login test is performed on the server that is shown in the IP Address column of External Server by using its set User ID and Password However it is necessary as a premise that the User ID and Password have been saved by pressing SAVE If the server cannot be logged in check settings on the PC side and those of the DS L1 SL N ETWO RK VI Basic Operations 4 Using the DS L1 via a Network 4 6 3 Saving Image Files in the FTP Server Image files can be saved in the FTP server by using either the CAPTURE button or the REC menu 4 6 3 1 Using the CAPTURE Button to Save Image Files Saving image files in the FTP server The CAPTURE button on the DS L1 main unit or the DS Remote Controller DS RC may be used to save image files in the FTP server Before you can do this you must first select Save to Sever for Capture Function in Additional Settings of the SETUP menu About the directory in which to save and the file name When an image file is saved in the FTP server using the CAPTURE button the file is stored in the folder specified on the DS L1 side at a location below the home directory of the server PC Also the file is automatically assigned a file name according to settings on the DS L1 side The folder names and file names are set in File Settings of the SETUP menu For file names we recommend selecting Auto for File Name so that files will be named after the dates and times at which they
208. unction key assignments on the USB keyboard Fi CAM menu F2 VIEW menu F3 REC menu F4 TOOL menu F5 F6 MIC menu F7 F8 F9 E ZOOM F10 E ZOOM F11 FREEZE F12 CAPTURE Back Space Deletes one character during character entry SHIFT Backspace Deletes the entire text during character entry NOTE The DS L1 is factory set to a Japanese keyboard layout If you wish to use an English keyboard issue the Telnet command shown below to change keyboard settings To revert to the Japanese keyboard use the Telnet command shown below to change keyboard settings w_KYBUS Selects an English keyboard w_KYBJP Selects a Japanese keyboard Settings you ve changed will take effect upon restarting the DS L1 after executing the command For information on Telnet connections refer to 4 5 Using Telnet Commands for the DS L1 in Chapter VI 24 VI Basic Operations SETU P 1 Preparations Before Photographing 12 Notes on Using a CF Card Images can be saved to or reproduced from a CF card Pay attention to the following when using a CF card Use a Type I or Type Il compliant CF card or microdrive Carefully read the user s manual provided with your CF card before using the card To format a CF card on your computer use FAT12 FAT16 Sometimes these are expressed simply as FAT or FAT32 Depending on the OS used you may not be able to select a format The DS L1 is able to format a CF card CF cards preformatted in NTFS th
209. units The pixel size of 5M cameras is 3 4 um so the scale can be calculated based on the optical magnification of the camera The pixel size of 2M cameras is 4 4 um so the scale can be calculated based on the optical magnification of the camera The scale for real 10 printing is a scale of 10 mm on a printed image Comment Select the Append check box for Log on the REC menu and enter a comment in the input box The comment will appear on the image being printed Comments can be up to 32 characters It is convenient to record the subject name number and the objective magnification for confirmation at a later time NOTE You can use the Telnet command to select whether to print information at the bottom of the printed image Do not print information w_PSTROF Print information w_PSTRON Overlay print Pen drawing text count marker and superimpose are saved overlapping on top of the image in the same position as displayed on the screen The scale and crossline are saved as set on the REC menu When the Scale or Cross check box is selected it appears over the image when deselected it can be saved without appearing over the image being displayed on the screen When selecting a PictBridge supporting printer only Selecting a Source described above is enabled Printing information at the bottom of the printed image is not possible In addition the settings for printing such as the paper size and paper type and so forth a
210. ure time using DF FL scene mode is recommended For operation method of DF FL scene mode refer to 2 1 2 Special Photography on the Easy Menu DF FL Scene Mode in Chapter IV 4 Correcting exposure Use CMP to correct exposure Setting CMP to the positive side makes the screen brighter 5 Confirming the image Press IL I freeze button and the screen will be switched between Freeze and Live While in Freeze or Live confirm the image you are photographing For photographing from DF FL scene mode refer to 2 1 2 Special Photography on the Easy Menu DF FL Scene Mode in Chapter IV 43 CAM ERA VI Basic Operations 2 Photographing Images 6 Saving the image Use Capt button or the CAPTURE button on the DS L1 to save the image Capt button and the CAPTURE button on the DS L1 are functionally the same The image is saved to the destination that has been assigned to the button You can also use these buttons to print the image Look at the LED located by the side of the CAPTURE button on the main unit to confirm the destination to which the image will be saved NOTE The CAPTURE button can be assigned any function by Capture Function on Additional Settings whether it be saving to a CF card saving to an FTP server or outputting directly to a printer Refer to 1 4 1 Date and Power save Settings Additional Settings in Chapter VI for details The image is saved in accordance with the File Settings with reg
211. uring normal operation V Photographing V Photographing WV Photographing Int set value Int set value Int set value Save Save Save When set intervals cannot be maintained V Photographing V Photographing Int set value Int set value Int set value AE SAVE iinis ann Seas Savos ase 60 VI Basic Operations CAM ERA 2 Photographing Images 2 2 2 Reproducing Images from a CF Card VIEW Menu Display a menu and select the VIEW tag on it to enter the VIEW menu In the VIEW menu you can reproduce images on the screen from a CF card You also can delete image files from the CF card or display a thumbnail CAM VIEW REC JK TOOL SETUP me PAIN SHIN lx DSO80803 lt DIR gt Locate the file name or folder name you DsosGshd4 lt TR gt want to reproduce at the gt position DnSOS0305 lt DIR gt DSOS0306 lt DIR gt IMGEOE lt DIR gt NIKON lt DIRe SAMPLE lt DIR gt hd Reproducing an image Images can only be reproduced in BMP and JPEG file formats Locate the file name you want to reproduce at the gt position and press gt playback button to reproduce the image on the screen A folder or file can also be selected directly with a mouse If any folders or files are not displayed on the menu use VJ or A to scroll the display up or down To use the panel switch or keyboard to scroll move the cursor to V or A and press the E
212. use harmful interference in which case the user will be required to correct the interference at his own expense This class A digital apparatus complies with Canadian ICES 003 Cet appareil num rique de la classe A est conforme a la norme NMB 003 du Canada 1 0
213. will be displayed 2 Press the red blue green black white or yellow icon to determine the color of the marker 3 Click any position on the screen and markers will be displayed Numbers 1 99 are displayed on markers with counts also shown to the right of the color icons If you wan to change the marker color in the middle of counts press your desired color icon That way you can count points independently for each color Or you can return to one of the colors you previously used and continue counting from the last count of that color If you failed to mark any point you can use or to cancel or redo your most recent action Up to 10 recent actions can be retrieved Use to erase all the content you ve now entered All markers you ve attached will be erased unless you have pasted NOTE If you put a marker in the middle of or Redo recent actions from that point in time cannot be canceled or redone 4 After marking points press to fix the markers you ve attached as valid data 5 Press to return to the previous menu Any markers you ve attached will be erased unless you have pasted Once the content is fixed as valid you cannot erase without erasing the entire overlay NOTE The count values cannot be saved The display information is interlocked with the menu size Small markers are displayed when you are showing a small menu and large markers are displayed when you are showing a large menu 69 3 3 3
214. will be displayed Select the icon ZELKO g Undo fe Redo a Clear a Paste S 2 Press a color icon red blue green black white or yellow to set the color of the measurement line 3 Draw a reference line Click the mouse button at the starting point of the reference line To draw the line hold the button as you drag the mouse Release the button at the ending point 4 Click at the position of a point located at the desired distance from the reference line A perpendicular line will be drawn from where you click to the reference line This is the measurement line The length of the measurement line will be displayed in the vicinity of it Each measurement line is assigned numbers 1 to 9 The number of measurement lines drawn in the same color is limited to nine To change colors click another color icon so that measurement lines drawn in that color will be assigned numbers separately from those in other colors To redraw a line use or to to cancel or redo your most recent action Up to 10 recent actions can be retrieved Use to erase all the content you ve now entered All lines drawn before confirmation with will be erased NOTE ii If you draw a measurement line in the middle of or Redo recent measurement lines drawn from that point in time cannot be canceled i To redraw a reference line select the icon once again 5 TO save a measurement line to the overlay of the image press to fix after drawing it
215. xternal device into the EXTERNAL OUT connector of the DS L1 make sure the power for the external device is turned off If the power for the external device remains on your eyes may be damaged by unintended light emission 8 Connecting to a Power Supply To prevent electric shock turn off the power for the DS L1 before making any connections Complete all other connections before plugging into a power supply When connecting the DS L1 to a power supply always use the specified AC adapter DS AC adapter to which a dedicated power cord should be connected Using the wrong AC adapter or power cord may lead to a fire or other dangerous conditions This product belongs to electric shock protection class I and must be connected to a power outlet with a protective ground terminal If you misplace or damage the DS AC adapter power cord please contact your nearest Nikon representative 2 Nees V Microscope Adjustment Microscope Adjustment When the camera head is mounted on a microscope adjust the microscope as described below 1 Use the Appropriate Filters When capturing a color image Insert the NCB filter into the optical path When capturing a black amp white image Insert the appropriate filter into the optical path depending on the photographic purpose Generally inserting a green interference filter GIF will heighten contrast Contrast is enhanced by inserting a filter with a color complementary to the specimen
216. y Confirm that you are within the controllable exposure range with a level meter or an error message Use a level meter or an error message on the upper right corner of the screen to check the drift from the exposure level that the DS L1 has determined to be optimum Because the exposure level considered to be optimum varies with each subject the level determined by the DS L1 should be regarded as an approximate guide The optimum range for the level meter is indicated by a green bar As the exposure becomes excessive it changes color to red In cases of underexposure it becomes a sky blue bar An Exposure Over or Exposure Under message will be displayed if overexposure or underexposure is recognized 53 CAM ERA VI Basic Operations 2 Photographing Images If a red or sky blue bar is displayed during automatic exposure AE you are outside the exposure controllable range Therefore adjust the amount of incident light by adjusting the luminous energy of the light source or the iris diaphragm or by using an ND filter Confirm the effective level of the photographed image from image distribution with a histogram The DS L1 calculates image distribution from a frozen image and displays it as a histogram HISTOGRAM This function cannot be used unless you use a mouse Right click W freeze button in the Advn menu with the mouse and the DS L1 will start calculating image distribution with a histogram di
217. y however Example If the home directory is C DSFTP the local path in the user account profile of the DS L1 is C DSFTP DS1 and the FTP Dir on the DS L1 side is FTPIBOX then the FTP transfer directory is C DSFTP DS1 FTPIBOX 2 Set local paths for the second and the third users DS L1s as C DSFTP DS2 and C DSFTP DS3 That way you can specify a directory separately for each user 4 6 1 2 When Using Windows 2000 Professional and XP Professional The following explains how to set FTP functions in Windows 2000 Professional and XP Professional The Control Panel is displayed in a different way on the two operating systems e In Windows 2000 you select Start Settings Control Panel e In Windows XP you select Start Control Panel Other than that almost all setting operations are common to both Setup procedure 1 Install Internet Information Service IIS Add FTP server functions 2 Adda user User ID and Password input Set the DS L1 as the login user 3 Specify the home directory Specify the home directory when logged in 4 Enable writing to the server Enable writing of image files to the FTP server 105 5 VI Basic Operations 4 Using the DS L1 via a Network Adding components If Internet Information Service IIS does not have components added you need to install them 1 2 3 Settings Open Control Panel and select Add R
218. yed on the screen pen count marker text two points distance measurement circular perpendicular angular measurements or superimposition is deleted when you switch the objectives If the microscope connected to your system is the Nikon 80i 90i microscope with digital imaging head the content drawn with the TOOL menu displayed on the screen pen count marker text two points distance measurement circular perpendicular angular measurements or superimposition is deleted when you change the magnification of the optical zoom If the microscope connected to your system is a Nikon modular microscope with a motorized nosepiece the content drawn with the TOOL menu displayed on the screen pen count marker text two points distance measurement circular perpendicular angular measurements or superimposition is deleted when you switch the objectives 65 TOO L VI Basic Operations 3 Measuring Two point Distance or Entering Information TOOL Menu Configuration of the TOOL menu CAH VIEW REC 0 TOOL SETUP Me Llear All Eq verlay OFF Marker Clear Al Overlay ON OFF D D x g 115 er Erases overlay information Displays or hides overlay Pen function Count marker function Text input function Enters a calibration value Calls calibration settings Measurement functions Displays a scale Displays an XY scale Crosshairs Display Measurement functions si s2 s3l s4 ssl sels7l acim wf D Scale

Nikon DS-2MBWC Digital Camera User Manual

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