BioChain Institute Inc.
Contents
1. wear appropriate gloves and a mask since the device may be contaminated Important Before starting extraction put on appropriate gloves a mask and safety goggles if required by the operation In the operation from preparing samples to extraction completion be careful not to contaminate the samples with sweat saliva etc www biochain com Page 17 Ver 1 14 2 1 Turning on the power 1 Make sure that the power cable are connected securely to the the AnaPrep 12 system 2 Connect the plug of the power cable to the outlet 2 2 Preparation The following preparations are required for extraction operation mM Items to be prepared Gloves Mask Safety Goggles Reagent Cartridge Reaction Chamber Tip Holder Pestle Option Filtered tip Sample tube Elute tube www biochain com Page 18 Ver 1 14 Piercing Pin mM For the preparation of samples refer to the handbook of each kit Provided with the Reagent Kits 2 3 Extraction 1 Turn the power switch on and waiting for the LCM screen turn on and shows AnaPrep 12 System Stand By 2 Press the Start button The system will process self testing and then go to steady mode Note The system will block main functions before the completion of self testing process 3 Open the sliding door and remove the sample rack from the instrument www biochain com Page 19 Ver 1 14 kee gt gt gt z 4 Load Rea2. 12 system 2 Connect Barcode Reader with the Instrument Ver 1 14 www biochain com Page 13 3 How to remove the fixing stuff inside the instrument following the steps below i Plug in the power cord to the instrument and connect to electric outlet ii Turn the power switch on iii Press Start button iv The piston module will going up and you can release the fixing stuff www biochain com Page 14 Ver 1 14 1 5 Overall View E Front view with Sliding Door closed LCD Screen 9 eg Sliding Door a gt Moving Handler E Front view with Sliding Door opened UV Lamp Operational Area RON Sal N E LCM Panel and Control Pad www biochain com Page 15 Ver 1 14 LCM Control Pad LCM displays information of operation instruction guide and program process status Control pad is used for selection of functions and input of program and test codes Turn on and off UV Light Pause or terminate the program Start the program wake up from idle mode Cancel Number Confirmation Previous Step buttons Next Step www biochain com Page 16 Ver 1 14 Back View Power Switch Power Inlet 1 x Barcode Reader Port amp 1 x USB Port 2 Getting Started A Biohazard Always wear appropriate gloves a mask and safety goggles during any biohazardous operations in extraction process Even when touching the device after any operation with a biohazard risk
3. BioChain Institute Inc J BioChain Instruction Manual Automated Nucleic Acid Preparation System AnaPrep 12 ka Catalog No Z1320001 Rev Date 19 Aug 2013 Manual No Z1001 Version 1 14 For General Laboratory Use Only TABLE OF CONTENTS Page INTRODUCTION ciccione a ees 3 SAFETY INFORMATION dees euglereeube ege 4 1 0 INSTALLATION seccsistecscseeedencscntasdassavsavenanewaistevedeccsuesoucdes eas tueciastasiesiieisiieseensas 8 1 1 Composition of the AnaPrep 12 Gvstem 8 1 2 CONTENTS OF REAPCNU KITS E 9 1 3 Operating Environment Condition ec cccccceeceseeesseeeseeeeseseneseuees 10 1 4 Unpack the AnaPrep 12 System ccsccsssssccussosssesccussossnesccussoussasceuss 12 L3 e NIT EE 15 2 0 GETTING STARTED ege 16 2 1 Turning ON the power 17 Ee Propa ge WEE 18 E de le EE 19 2 4 After Extraction ODE e E 25 3 0 Cleaning amp Maintaining the AnaPrep 12 Instrument csccscsccsceccscecees 26 4 0 Troubleshooting crcs a aa aa 27 EI REESEN 29 ENEE 30 www biochain com Page 2 Ver 1 14 INTRODUCTION Thank you for purchase of the AnaPrep 12 system which is a fully automated standalone robot that can purify nucleic acids within 30 45 minutes With advanced magnetic bead separation technology it enables you to have high quality extraction results Moreover the most user friendly interface makes users free from troublesome parameter settings and maintenance This guide contains importa
4. ample Tray Make sure the Sample Tray be placed correctly in the instrument 8 Close the Sliding Door and press Start button 9 Scan the protocol barcodes to select purification protocol sample volume and elute volume Note There is one protocol barcode paper enclosed in the reagent kit box Protocol s name sample volume and elution volume will be shown on LCD screen after protocol barcode is scanned 10 Follow the instructions displayed on LCM screen to double check the operating steps being completed before program running www biochain com Page 24 Ver 1 14 2 4 11 Push Enter to confirm Instrument will start to run the protocol program automatically until whole processes are completed Note It takes from 30 to 45 minutes to complete the extraction according to reagent types 12 At the end of the run the instrument beeps briefly and the LCM shows Protocol Completed 13 Open the instrument door 14 Remove the elute tubes containing the purified nucleic acid Note Store the purified nucleic acids at 4 C for short term storage or store at 70 C for long term storage 15 Discard the used cartridges all plastic consumables into biohazard waste Do not reuse the cartridges 16 If you re not using the instrument place the Sample Rack back to workplace close the instrument door and push Start button for 2 secs to get into sleeping mode And for longer ti
5. e a failure or malfunction of the AnaPrep 12 system If any liquid materials are left inside the device wipe it up a soft paper tissue etc Otherwise the AnaPrep 12 system may be damaged www biochain com Page 5 Ver 1 14 Repairs to the AnaPrep 12 system should only be performed by such agencies as are specifically authorized by the BioChain Institute Inc Only original the BioChain Institute Inc replacement parts should be used If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired About Reagent Kits A CAUTION E When handling any of the kits refer to the respective handbook M Reagents in each kit should be handled by observing the safety information and precautions regarding the kit M Extraction should be performed in an appropriate laboratory or workplace Note The kits are not supplied with the AnaPrep 12 system Select the desired kit s and order it them separately www biochain com Page 6 Ver 1 14 About Samples AN BIOHAZARD mM Always wear appropriate gloves a mask and safety goggles etc when handling any infectious samples About Infectious Wastes E When handling or disposing of infectious materials follow the laboratory guideline or the law regarding infectious waste to perform proper incineration fusion sterilization and or disinfection E When you use a third party to dispose of it outsource this work an operato
6. gent Cartridges and all plastics disposables Reaction Chamber Tip Holder Piercing Pin Filtered Tip and Pestle optionally supplied with some kit types Insert the cartridges E How to pull apart reagent cartridges gt Slash open the dotted line with nail and snap it with a little bit force www biochain com Page 20 Ver 1 14 Insert Piercing Pins one row only www biochain com Page 21 Ver 1 14 hl LL N Fei A al de MESS sss c gt ea 7 N AN al Co Insert Filtered Tips one row only Note Load one Reagent Cartridge and one set of plastic disposable per sample Important Set Cartridges in the order of the number from left to right Make sure that Cartridges are inserted in to the Cartridge Tray tightly You can load 1 12 cartridges on the tray depending on the number of samples that you wish to process 5 Load Sample Tube and Elute Tube to Sample Rack on the bench Insert Sample Tube on to Sample Rack www biochain com Page 22 Ver 1 14 Insert Elute Tube on to Sample Rack 6 Load the sample s to Sample Tube Pretreatments are essential for some sample types before loading to Sample Tube Please refer to the handbook of reagent kits for details Make sure the caps of Elute Tube are open as the figure shown above 7 Place Sample Rack on the instrument platform www biochain com Page 23 Ver 1 14 DE Use two hands to handle the S
7. ion at the bottom of the well Ensure that the lysate does not contain any particulate material Clogged Tips resulting in that can clog the tip sprout If needed centrifuge the sample DNA loss prior to the AnaPrep purification Store cartridge containing the beads at room temperature Do not freeze the cartridge as the beads may be irreparably Magnetic beads stored or No DNA recovered damaged handled improperly Make sure that the beads are in solution at all times and do not dry Dried beads are non functional Remove any magnetic beads using a magnetic separator or Magnetic beads present Eluate containing centrifuge the sample in a microcentrifuge for 1 minute at in the eluate DNA maximum speed is discolored DNA contaminated with Minimize the amount of blood or blood stained sample used lt heme 20 ul blood spot for forensics sample To prevent bubble formation during mixing make sure the Bubbles formed during sample volume is at least the recommended volume listed mixing steps in the manual supplied with Reagent Kits DNA is sheared or Purified DNA repeatedly Aliquot purified DNA and store at 4 C short term or 20 C degraded frozen and thawed long term Avoid repeated freezing and thawing DNA contaminated with Maintain a sterile environment while working i e wear gloves DNases and use Dnase free reagents www biochain com Page 28 Ver 1 14 5 Specifications Instrument Type Sam
8. its enclosed in reagent box for details 1 3 Operating Environment Condition Use the AnaPrep 12 system in a location that meets the following conditions owe M The space for the allocation of the AnaPrep 12 system system must be remained 5 10 cm from instrument to side walls A location where power can be provided E A location where the temperature is 10 to 40 C and humidity is 30 to 80 RH non condensing E A location that is flat and stable with no vibration www biochain com Page 10 Ver 1 14 E A location away from direct sunlight Block the sunlight by closing curtains or blinds as necessary A location which is well ventilated and not dusty A location where the temperature does not go up and down suddenly Warming a cold room suddenly or moving the AnaPrep 12 system from a room with low temperature to a warm room may cause condensation inside the device resulting in abnormal extraction E A location where the temperature and humidity are kept within the specified range far from water taps water heaters humidifiers air conditioners and heaters WR A location far from objects which generate strong magnetic fields motors transformers TV audio speakers magnets etc Bringing the AnaPrep 12 system close to any type of magnetic field may cause a malfunction Warning Do not use the AnaPrep 12 system to a location where it is wet or can be splashed with water It may cause a device failure fire
9. me not using the instrument turn the power switch off After Extraction Operation 1 Take out the Elute Tubes The extraction yields in them Note i You can apply quality checking or do downstream study or storage them as what you expect 2 Remove the Cartridges and plastic disposables from the instrument and then dispose of them www biochain com Page 25 Ver 1 14 3 Cleaning amp Maintaining the AnaPrep 12 system Two types of maintenance are performed on the the AnaPrep 12 system Instrument as listed in the table For details on each type of maintenance see below Maintenance Type Performed by Schedule Routine 17 Cleaning of Sample Tray After each use 18 Cleaning of instrument Bi weekly body outside 19 Cleaning of instrument Bi weekly body inside Preventive Service Engineer Annually E Clean the Sample Tray with mild detergent and rinse with deionized water Allow the parts to dry before use Clean and disinfect the platform surface by wiping with deionized water followed by 70 ethanol M Clean the instrument body by removing dust gently with a dry soft cloth If the outside of the AnaPrep 12 system is heavily soiled or if any samples that may cause infection are adhered to the outside of the AnaPrep 12 system wipe with a soft paper tissue soaked with 0 5 sodium hypochlorite solution or ethanol www biochain com Page 26 Ver 1 14 4 Troubleshooting ees Je Jee Instrument Problem
10. nt information regarding the safe use of the AnaPrep 12 system Please read this manual carefully before you start to run the system at the first time especially for Safety Information If there is any question about how to install or operate it please contact our certified distributors agents or email to our technical support center techsupport biochain com Contact Info Company BioChain Institute Inc Address 39600 Eureka Dr Newark CA 94560 USA Tel 1 510 783 8588 Fax 1 510 783 5386 Mail info biochain com AnaPrep Magnetic Bead Preparation Process Ke Beads 0 e of Si M www biochain com Page 3 Ver 1 14 SAFFETY INFORMATION The meanings of safety precaution marks are as follows WARNING WARNING indicates a dangerous condition that may lead to death or serious injury BIOHAZARD This symbol is used to indicate that certain precautions must be tken when working with potentially infectious material CAUTION This symbol is used to indicate that non compliance with instructions or procedures may lead to physical injury or even death or could cause damage to the instrument Important Important shows the important notes for usage as well as prohibited actions Note Note indicates the notes procedures that should be obeyed and supplementary information for use HOT SURFACE This symbol is used to label potentially hot instrument surfaces For y
11. or electric shock When relocating the AnaPrep 12 system disconnect the plug from the outlet first If the power cable is damaged this may cause a device failure fire injury or electric shock AN Caution Do not use the AnaPrep 12 system in an unstable place such as a slanted surface or a place subject to vibrations It may cause injury or device failure Do not use the AnaPrep 12 system in direct sunlight or close to a heating device It may shorten the life of the AnaPrep 12 system or cause a trouble www biochain com Page 11 Ver 1 14 Operating Conditions jem Cie 1 4 Unpack the AnaPrep 12 system 1 Open the packing box and take out the instrument and related accessories Important The AnaPrep 12 system has a weight of more than 45 kgs It should be lift and moved by two persons Hold the moving handler of the instrument from two sides to move it out from the box HM Do not hold the plastic outer covering E Do not hold the front panel www biochain com Page 12 Ver 1 14 E Do not hold the door A Caution Improper handling of the movement of the AnaPrep 12 system will lead to instrument damages For correct and safe use of AnaPrep 12s install it in a location that is close to electrical outlet and has enough space for installation and operation of the mains switch Important Keep the shipping box and stuffing materials They will be needed again when transporting the AnaPrep
12. our safety and that of others follow the guidelines provided in the following pages concerning the use of the AnaPrep 12 system www biochain com Page 4 Ver 1 14 About Instrument WARNING lgnoring the following notations may lead to fire or electric shock In countries other than US and Canada use a power cable that meets your country s standard or contact your local distributor Do not use the AnaPrep 12 system with voltage other than the voltage specified on the device Do not use the AnaPrep 12 system with a damaged power plug ora loose socket If there is dust on the prongs of the power plug or on the plug socket remove it with a dry cloth When you disconnect the plug from the outlet be sure to hold the power plug itself Do not pull the power cable For maintenance disconnect the power plug from the outlet Do not touch the power plug when you hear the crash of thunder Do not pour any liquid on the AnaPrep 12 system Do not place any objects containing liquid on the AnaPrep 12 system Doing so may cause a device failure fire or electric shock In the event the device overheats starts to smoke or smells strange immediately unplug the power cable A CAUTION Never attempt to remodel the AnaPrep 12 system without the manufacturer s permission Doing so may cause fire or electric shock Do not place or drop objects on the AnaPrep 12 system Also refrain from bumping or knocking it as doing so may caus
13. ple Processing Sample Volume Handling Processing Time Heat Block Temperature Protocol Input Built in Features Instrument Dimensions Input Power Operating Temperature Operating Humidity Fuse Temperatures allowed during transportation storage packaging CE LV directive 73 23 EEC EMC directive 89 336 EEC EMC EN61326 1 Safety EN60101 1 EN60101 2 www biochain com Page 29 30 80 F3A 250V 25 Cto 70 C Ver 1 14 APPENDIX 20 Replacement Optional Parts Filttered Tip A02001 21 www biochain com Product Guarantees The guaranty period shall be one year from the delivery of the product Any part which fails to function properly under normal use provided that all warnings and cautions in this manual are observed during the guarantee period will be repaired by the BioChain Institute Inc free of charge If the problem is due to the causes listed below some charge may be applied for the repair even within the guarantee period Any failures due to improper use or that are affected by other devices except for those approved by the BioChain Institute Inc Any malfunctions or damages during transportation or due to dropping of the product by a user Page 30 Ver 1 14 22 About Services Before you ask for device repairs refer to 4 Troubleshooting to check the problems If the problems cannot be solved contact your local representative or the relative cu
14. r licensed to handle medical waste subject to special control and give them the manifest of the medical waste at the same time BioChain Service Center M For technical problem and instrument maintenance please contact our service center BioChain Institute Inc 39600 Eureka Dr Newark CA 94560 USA 1 510 783 8588 1 510 783 5386 techsupport biochain com www biochain com Page 7 Ver 1 14 1 Installation 1 1 Composition of the AnaPrep 12 system Check that the following are included in the package Contact your local representative if you notice any missing part s Q ty A AnaPrep Instrument 1 B Power Cord 1 C Sample Tube Rack 1 Ver 1 14 www biochain com Page 8 D Barcode Reader 1 E User s Manual 1 Please make all the components are free from damages as soon as you get the system If any damage found please contact your local representative for the instant support Note BioChain s global warranty does not cover the damages from the transportation or improper operation 1 2 Contents of Reagent Kits A Reagent Cartridge s B Reaction Chamber s C Tip Holder D Piercing www biochain com Page 9 Ver 1 14 E Filtered tip F Sample tube G Elute tube H Pestle Option for some Kit types Note Reagent kits are separated purchase Please contact your local agents or representatives to get further info The contents of reagent kits will be various Refer to the handbook of k
15. s No power the LCD Screen remains blank when the power is turned on LCD Screen turns on when the power is on but the self testing program does not run Protocol stops after an initial start Bubbles formed during extraction Presence of buffer in the Cartridge Tray Leakage from Filtered Tips or uneven liquid handling between Filtered Tips Blockage of tips and pipetting failure www biochain com AC power cord is not connected Forget to remove the packing stuff from instrument Technical problem Cartridge s Plasticwares Reaction Chamber Tip Holder Filtered Tip Pestle Sample Tube Elute Tube incorrectly loaded on the the AnaPrep 12 system Problem with motion sensors Missed adding sample or sample volume is lower than the recommended volume Motor movements may not be smooth incorrect placement of plasticware or leakage from tips Air leakage on the Filtered Tip Too much starting material or excess DNA in sample causing clumps Page Zi Check AC power cord connections at both ends Or Use the correct cords Turn off the instrument and remove the packing stuff Contact your local representative or agent Turn off the power and then turn it on again to stop the program The system will move back to initial state Re load them according to the instructions shown in this manual Note you could not resume the protocol after stop and you may lose your
16. samples Turn off the power and remove all samples and plasticwares Contact your local representatives Be sure to add the sample to tubes prior to starting the protocol To ensure proper mixing of reagents in the tip and prevent bubble formation during mixing make sure the sample volume is at least the recommended volume listed in the handbook supplied with the AnaPrep Reagent Kits Perform preventive maintenance annually to ensure proper motor movements Swap the air leaked Tip with new one 1 Decrease the amount of starting material Use the recommended amount of starting material as listed in the Reagent Kit manual Handbook Ver 1 14 or aggregates 2 Suggest using blood kit 1200 instead of blood kit 200 if testing sample is blood DNA Quality Problems Decrease the amount of starting material used Be sure to add Proteinase K during lysis if included in the Incomplete lysis protocol Make sure that the sample is completely immersed in the Lysis Buffer Be sure to process sample immediately after collection or store Poor quality of starting the sample at appropriate temperature The yield and the l material Low DNA yield quality of DNA isolated depend on the starting material During shipping some magnetic bead solution may adhere to Insufficient amount of the sealing foil of the cartridge To collect any bead solution magnetic beads added from the foil tap the cartridge to deposit the bead solut
17. stomer support 23 Notes for Transporting Shipping the AnaPrep 12 system When transporting shipping the AnaPrep 12 system to a new location perform the following tasks Warning Before carrying the AnaPrep 12 system disconnect the plug from the outlet If the power cable is damaged this may cause a device failure fire injury or electric shock When transporting shipping the AnaPrep 12 system be sure to perform the following tasks Remove all attached parts Sample Rack Barcode Reader from the AnaPrep 12 system After completing the above tasks pack the AnaPrep 12 system in its original shipping box or some other equivalent box www biochain com Page 31 Ver 1 14 24 Instrument fixing stuff before Transport 1 Press the button 0 3 Press the button 5 4 Open the sliding Door www biochain com Page 32 Ver 1 14 5 Putting the Fixture on the Position of Tip Holder 6 Close the sliding Door 7 Press Enter button PLACE FIXING SLO lt EMNTER gt GO eso 8 Finish www biochain com Page 33 Ver 1 14 BioChain Institute Inc www biochain com BioChain Institute Inc 39600 Eureka Dr Newark CA 94560 USA 1 510 783 8588 1 510 783 5386 techsupport biochain com www biochain com Page 34 P Biochai J BioChain Ver 1 14
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