User`s Manual
Contents
1. but it should be rinsed off and dried once the o ring is in place Page 22 Cyclops Submersible Sensor 2 Slide the Tightening Cap on from the plug end of the Cyclops 7 and the Shade Cap from the optical end of the Cyclops 7 as shown below positioning the threads over the o ring Turning clockwise hand tighten the Tightening cap onto the Shade Cap The Shade Capis installed correctly if the optic end of the Cyclops 7 is flush with the interior base of the Shade Cap 3 Optional Turning clockwise hand tighten the End Cap to the other end of the Shade Cap until it is fully secured to the Shade Cap The End Cap prevents sediment and other debris from entering the shade cap Shade Cap Specifications Weight 110 g 0 24 lbs Length 12 7 cm 5 0 in Diameter 4 3 cm 1 7 in Depth Rating 600 meters 998 2100 Rev 2 0 Page 23 End Cap Cyclops Submersible Sensor Introduction 1 The Shade Cap P N 2100 708 for the Plastic Cyclops 7 Sensor offers protection for the optics located at the optical end of the instrument It prevents damage from deploying recovering or transporting the instrument in fast flowing environments and or from bottoming out in shallow environments The Shade Cap also helps block other possible sources of light that may interfere with the Plastic Cyclops 7 Sensor s measurements Features 2 e Threaded ends for easy installation and removal e Corros2. 10 Meter Pigtail Cable with Locking Sleeve P N 2100 751 25 Meter Pigtail Cable with Locking Sleeve P N 2100 752 50 Meter Pigtail Cable with Locking Sleeve P N 2100 753 6M Any of the cables listed above may be used to operate the Cyclops Color Pin Function Connection a eT ES oe E n e Red 1 nn PSU Positive Connection 3 15 VDC Supply Ground Black 2 PSU Ground Connection 0 VDC Signal Out to data logger White 3 Positive Connection 0 5 VDC Analog Ground 7 0 VDC LCELEEELENE CEE EEE CRETE CEE EEE EEE EEE FE EEE FFE EEE EEE EE Se eni 998 2100 Rev 2 0 Page 18 Cyclops Submersible Sensor Appendix E Pigtail Cable and Connector Information Dimension details of 24 length cable with 20 gauge colored lead wire connects to 6 pin male connector Cable manufacturer Part No IMPULSE MCIL 6 FS SE a ae ane mee Baan y aiin oin i ae E Se PE M a at a Por a S TE Fa a a 2 ne eu m7 mi a a oe Ce Fa Rene ne Se ee tas PERS FRE RENE NEOPRENE Female locking sleeve Impulse P No MCDLS F 29 MATED 7 OQO NEOPRENE In line connector contact configuration connects to Cyclops le SN sex 1 O RING 2014 7116 20 UNF 24 12 TEFLON BRASS LEADS STAINLESS STEEL A maximum cable length up to 300 meters can be connected to the Cyclops if the following conditions are met 1 The cable is shielded and contains 20 gau
3. e Cyclops Pigtail Cables with Locking Sleeve see Appendix D for more information 2 foot Pigtail Cable with Locking Sleeve P N 2100 750 5 meter Pigtail Cable with Locking Sleeve P N 2100 755 10 meter Pigtail Cable with Locking Sleeve P N 2100 751 25 meter Pigtail Cable with Locking Sleeve P N 2100 752 50 meter Pigtail Cable with Locking Sleeve P N 2100 753 e DataBank Handheld Data Logger see Appendix K P N 2900 000 e Flow Cap see Appendix for more information Cyclops 7 Stainless Steel and Titanium P N 2100 600 Cyclops 7 Plastic P N 2100 608 Cyclops 6K P N 2160 600 e Shade Cap see Appendix H for more information Cyclops 7 Stainless Steel and Titanium P N 2100 701 Cyclops 7 Plastic P N 2100 708 Cyclops 6K P N 2160 700 e Solid Secondary Standard SSS for in vivo Chlorophyll Phycocyanin Phycoerythrin Rhodamine Fluorescein see Appendix J for more information Cyclops 7 Stainless Steel and Titanium P N 2100 900 Cyclops 7 Plastic P N 2100 908 Cyclops 6K P N 2160 900 e Solid Secondary Standard SSS for UV Sensors CDOM fDOM Optical Brighteners Refined Fuels and Crude Oil see Appendix J for more information Cyclops 7 Stainless Steel and Titanium P N 2100 904 Cyclops 7 Plastic P N 2100 905 Cyclops 6K P N 2160 901 Solid Secondary Standard 7 4 2 Li g E fe S S g Shade Cap Flow Cap 998
4. in their case NOTE EPA Method 445 0 in vitro when not in use and kept free of dust and determination of chlorophyll in moisture Special care must be taken with the algae can be found on Turner UV Solid Secondary Standard P N 2100 904 Designs website to ensure that it is not exposed to UV light for prolonged periods of time This can result in 4 The Solid Secondary Standard s signal is degradation of the standard now equivalent to the concentration value determined from step 3 and can be used in place of a liquid primary standard for future calibration of that specific Cyclops 7 Fluorometer Use of the Solid Secondary Standard for Dye Tracing Applications The Solid Secondary Standard can also be used to check fluorometer stability when making dye concentration measurements If necessary the Solid Secondary Standard can be used to establish a new correlation voltage without using a calibration solution each time 998 2100 Rev 2 0 Page 31 Adjustable Cyclops Submersible Sensor Introduction Installation The following information describes how to use the Plastic Cyclops 7 Solid Secondary 1 Align Solid Secondary Standard with Standards P N 2100 908 In Vivo Chlorophyll 2 Rhodamine WT Fluorescein Phycocyanin and Phycoerythrin P N 2100 905 CDOM fDOM Crude Oil Refined Fuels and Optical Brighteners Features e Can be used in place of a primary liquid standard once a correlation between a primary standa
5. values by calibrating with a standard of known concentration 998 2100 Rev 2 0 Page 4 Cyclops Submersible Sensor 2 Inspection and Setup 2 1 Instrument Checklist The Cyclops Submersible Sensor shipment package consists of e Cyclops Submersible Sensor Configured and factory scaled for the specified analysis see Identification Letter stamped on the connector for specified analysis C Chlorophyll R Rhodamine F Fluorescein P Phycocyanin E Phycoerythrin U CDOM fDOM O Crude Oil B Optical Brighteners T Turbidity A PTSA G Refined Fuels A PTSA e Cyclops Documentation Kit includes Users Manual Quick Start Guide in vivo Calibration Procedure Cable Guide e Calibration Certificate 2 2 Housing Configurations e Stainless Steel Cyclops 7 P N 2100 000 Identification Letter e Plastic or Titanium housings recommended for highly corrosive environments or long term deployments Titanium Cyclops 7 P N 2100 000 Identification Letter T Plastic Cyclops 7 P N 2108 000 Identification Letter Plastic Cyclops 7 with Titanium connector P N 2108 000 Identification Letter T e 6000 meter Cyclops 6K P N 2160 000 Identification Letter 998 2100 Rev 2 0 Page 5 Cyclops Submersible Sensor 2 3 Optional Accessories include
6. voltage from a blank sample for the configured gain setting Note A good blank to use for this application ts ultra pure or deionized water 3 Use a turbidity standard of Known concentration NTU and create a correlation between the standard NTU and its voltage output 4 Once acorrelation has been made use the following equation to calculate turbidity values for sample measurements for the calibrated gain NTUsample NT Usti Voltssta VoltSsiank VoltSsample E VoltSgiank NTUsta Concentration value of standard used for calibration NTUsampie Concentration of sample VoltSst Voltage reading from standard concentration VoltSsampie Voltage reading from sample s VoltSpiank Voltage reading from blank 998 2100 Rev 2 0 Page 11 Cyclops Submersible Sensor 5 Maintenance and Warranty 5 1 Maintenance 5 1 1 Rinsing The Cyclops should be rinsed or soaked in fresh water following each deployment ideally until it is completely clean again 5 1 2 Care for the bulkhead connector A light coat of Silicone spray should be used on the rubber of the male pins of the bulkhead to aid in sealing The manufacturer recommends 3M Silicone Lubricant Spray or Loctite 8021 spray Note You should avoid using silicone grease Do NOT use WD 40 it will destroy the connectors 5 1 3 Care for the optics The optical window should be visually inspected after each deployment following a soaking in fresh water If cleaning is n
7. 2100 Rev 2 0 Page 6 Cyclops Submersible Sensor 2 4 Functional Test To perform a functional check on the Cyclops connect the interface colored wires to the power supply and multi meter as shown in Figure 1 below Additional Equipment required for functional tests DC Power Supply 3 15 VDC gt 100 mA Multi meter to read 0 5 VDC Note Supply voltages greater than 15 VDC will result in damage to the sensor DC Power Supply Multimeter mL O DO PSU Positive Connection Supply Ground Red 0 VDC Black LED Light Output Signal Output White Blue Brown Tie to Green for 10X Tie to Green for 100X Analog Ground Green HS X1 Low Gain Leave both wires disconnected for Functional Check Figure 1 With the Cyclops connected as shown in Figure 1 answer questions 1 3 by making the following functional tests 1 Is the LED on Hold a piece of white paper about 1 2 an inch in front of the optical head to ensure the LED is on Note Cannot perform this test for Turbidity sensors because they use IR which ts not visible 2 Is there voltage output The multi meter should be reading some voltage gt 0 VDC 3 Does the voltage output change Move the light source closer to your hand or a surface and check if the voltage output increases 998 2100 Rev 2 0 Page Cyclops Submersible Sensor 3 Measurements with the Cyclops 3 1 Introduction The following information will
8. 7 998 2100 Position the compression gasket approx 2 inches from the optical end of the sensor It is important to check that the O Ring installed in the Flowthrough Body Assembly is squarely seated on the bottom of the cap It acts as a washer between the sensor and the cap Itis possible to damage the optical face of Cyclops 7 if this O Ring is not in place Insert the optical end of Cyclops 7 in the Flowthrough Body Assembly With the Cyclops 7 fully inserted into the cap slide the compression gasket towards the optical end until it bottoms out inside the Cap Place threaded end cap over the Cyclops 7 sensor and screw down tightly by hand do not use tools to tighten Page 26 Cyclops Submersible Sensor Summary The sensor and flowthrough cap are now ready for use Once installed the Cyclops 7 sensor should be oriented such that the flow through cap is facing up This orientation will prevent bubbles from collecting on the optics The inlet and outlet ports of the cap accept 3 8 ID tubing The inlet tubing should be connected to the side port on the cap and the outlet tubing connected to the top port Note With the Flowthrough Cap installed the blank offset for the sensor will be elevated If you are interested in measuring the offset increase DI water can be used to measure the blank before and after the cap installation 998 2100 Rev 2 0 Application Note For R
9. For greatest accuracy record the sample temperature and correct the sensor output for changes in temperature For further information on how temperature light water quality and the physiological state of the algal cells can all affect the measurement of chlorophyll please refer to the application section of Turner Designs website 998 2100 Rev 2 0 Page 21 Cyclops Submersible Sensor Appendix H Installing the Shade Cap Introduction The Shade Cap P N 2100 701 for the Stainless or Titanium Cyclops 7 Sensor offers protection for the optics located at the optical end of the instrument It prevents damage from deploying recovering or transporting the instrument in fast flowing environments and or from bottoming out in shallow environments The Shade Cap also helps block other possible sources of light that may interfere with the Cyclops 7 Sensor s measurements Features e Corrosion resistant material e Reduces interference from ambient light sources e Large flow slots for improved flow e Protects optics _ a Air purge slots Flow slots 9 Stainless or Titanium Cyclops 7 Sensor Installation 1 Install the Spacer o ring approximately 1 72 from the optical end of the Cyclops 7 The o ring is intended to fit very tightly on the Cyclops 7 and can be difficult to install You can use the Tightening Cap to help slide the o ring on Soapy water can be used to lubricate the o ring
10. Length 9 7 cm 3 82 inches 998 2100 Rev 2 0 Page 34 Cyclops Submersible Sensor Use of the Solid Secondary Standard for in vivo Chlorophyll Applications 1 Using your Cyclops 6K Fluorometer measure a sample containing algae and record the response and the gain values for that measurement 2 Dry off the optical end of the Cyclops 6K attach the Solid Secondary Standard to the fluorometer and adjust the Solid Secondary Standard to produce the same response in the same gain as in step 1 3 Perform a chlorophyll extraction to determine the actual chlorophyll concentration of the sample Note EPA Method 445 0 in vitro determination of chlorophyll in algae can be found on Turner Designs website 4 The Solid Secondary Standard s signal is now equivalent to the concentration value determined from step 3 and can be used in place of a liquid primary standard for future calibration of that specific Cyclops 6K Fluorometer 998 2100 Rev 2 0 Care and Storage Solid Secondary Standards should be stored at room temperature 20 degree C in their case when not in use and kept free of dust and moisture Special care must be taken with the UV Solid Secondary Standard P N 2160 901 to ensure that it is not exposed to UV light for prolonged periods of time This can result in degradation of the standard Page 35 Cyclops Submersible Sensor Appendix K Using the Cyclops Submersible Sensor with the DataBank Cycl
11. Plastic may fluoresce and interfere with the sample s fluorescence 2 If using a glass container place the container on a non reflective black surface 3 Ensure that the sensor is more than 3 inches above the bottom of the container 4 Ensure that the sensor is in the center of the container and has more than 2 inches clearance between the cirumference of the sensor and the inside surface of the beaker Calibrated Sensor gt 2 inches all round Glass Beaker gt 998 2100 Rev 2 0 Page 16 Cyclops Submersible Sensor Appendix C Wiring Guide ire a Voltage E w E E _ 15 VDC PSU Positive Connection ep Supply Ground OVDC PSU Ground Connection Signal Out 3 data logger Multimeter Positive White Connection 0 ee Green Analog Ground Multimeter Negative 0 VDC Connection Blue X10 Gain Medium See table below i ae Brown X100 Gain High See table below Sensitivity Gain Switching Table Gain 10 Gain 100 Chi Range SUN Blue Brown ug L pange Range 5 ppb NTU Not connected Not connected 0 500 0 1 000 0 3000 Connected to Not connected 0 100 0 1000 analog ground Not connected Connected to he 0 100 analog ground 998 2100 Rev 2 0 Page 17 Cyclops Submersible Sensor Appendix D Cable Guide Cyclops Pigtail Cable with Locking Sleeve 2 foot Pigtail Cable with Locking Sleeve P N 2100 750 shown 5 Meter Pigtail Cable with Locking Sleeve P N 2100 755
12. TURNER DESIGNS CYCLOPS SUBMERSIBLE SENSORS User s Manual May 8 2012 P N 998 2100 Revision 2 0 TURNER DESIGNS 845 W Maude Avenue Sunnyvale CA 94085 Phone 408 749 0994 FAX 408 749 0998 Table of Contents 1 Introduction 1 1 Description 2 Inspection and Setup 2 1 Instrument Checklist 2 2 Housing Configurations 2 3 Optional Accessories 2 4 Functional Test 3 Measurements with the Cyclops 3 1 Introduction 3 2 Setting the Gain 3 2 1 Gain Determination Procedure 3 2 2 Static Gain Control 3 2 3 Auto Gaining 3 3 Direct Concentration Calibration 4 Turbidity Cyclops 4 1 Introduction 4 2 Calibration 5 Maintenance and Warranty 5 1 Maintenance 5 1 1 Rinsing 5 1 2 Care for the bulkhead connector 5 1 3 Cleaning the Optics 5 2 Warranty Terms 5 3 Warranty Service 5 4 Out of Warranty Service Appendices Specifications Recommended Measurement Practices Wiring Guide Cable Guide Pigtail Cable and Connector Information Controlling the Gain Linear Range Quenching and Temperature Considerations Installing the Shade Cap Installing the Flow Cap Installing and Using the Solid Secondary Standard Using the Cyclops Submersible Sensor with the DataBank Optical Specification Guide ASTTOATMIMVOWS NO O1 O1 WO O WO OO 0 OC WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT WEEE DIRECTIVE Turner Designs is in the business of designing and selling products that benefit the well being of our environment Accordin
13. ail Cable and Connector information Also see Setting the Gain section 3 2 for more information on Static Gain Control Method 2 Dynamic Gain Control If you have a Data Collection System DCS that has programmable outputs you can use them to control the Cyclops gain settings Following are three common output types found in DCSs and how to connect them to the Cyclops Refer to your DCS manual to determine which is appropriate For those who want technical data the Cyclops s X10 and X100 gain control pins are connected internally to the input of a Schmitt trigger inverter part number 74LVC1G14 and a 100K ohm pull up resistor Both use a 5 Volt power supply Output type 1 Digital Signals Logic signals can be used to drive the gain control pins In most cases you can connect the digital signal output of the DCS directly to the Cyclops gain control pins To drive them high the voltage should be 3 VDC min 5 VDC max To drive them low the voltage should be 1 VDC max 0 VDC min You may need to connect the Cyclops analog ground to the DCS ground Output type 2 Open Collector Signals This type of output is either open or connected to ground Connect the Cyclops gain control pins directly to these outputs You may need to connect the Cyclops analog ground to the DCS ground Output type 3 Relays Relays act as a controllable switch Connect one end of the relay to the Cyclops analog ground Connect the other end o
14. ake the following steps 1 Write email or call Turner Designs Technical Support and describe as precisely as possible the nature of the problem Phone 1 877 316 8049 Email support turnerdesigns com Carry out any adjustments or tests as suggested by Technical Support If proper performance is not obtained you will be issued a Return Materials Authorization number RMA to reference Package the unit write the RMA number on the outside of the shipping carton and ship the instrument prepaid to Turner Designs If the failure is covered under the warranty terms the instrument will be repaired and returned free of charge for all customers in the contiguous continental United States For customers outside of the contiguous continental United States who purchased equipment from one of our authorized distributors contact the distributor If you purchased directly contact us We will repair the instrument at no charge Customer pays for shipping duties and documentation to Turner Designs Turner Designs pays for return shipment custom duties taxes and fees are the responsibility of the customer 5 4 Out of Warranty Service Follow steps for Warranty Service as listed above If Technical Support can assist you by phone or correspondence we will be glad to at no charge Repair service will be billed on a fixed price basis plus any applicable duties and or taxes Shipment to Turner Designs should be prepaid Your bill will includ
15. andard used for calibration Csample Concentration of sample VoltSsiqg Voltage reading from standard concentration VoltSsampie Voltage reading from sample s VoltSgian Voltage reading from blank 998 2100 Rev 2 0 Page 10 Cyclops Submersible Sensor 4 Turbidity Cyclops 4 1 Introduction The Turbidity Cyclops measures turbidity using an 850nm light source and detection of scattered light at a 90 degree angle which is similar to many modern day bench top turbidity meters This unit provides a quick and accurate way to determine in situ turbidity eliminating the collection and storage of samples and minimizing the potential error associated with sample handling and processing 4 2 Calibration Calibrating the Turbidity Cyclops is a simple process requiring the use of calibration standards Turner Designs recommends purchasing Amco Clear Analytical Turbidity Standards for non ratio instruments because these standards are non toxic safe solutions consisting mainly of deionized water that comes prepared in a broad range of concentrations and has a shelf life guaranteed for one year The Turbidity Cyclops can be calibrated using a single calibration standard which correlates the concentration to the voltage measured for that specific standard 1 Connect the Turbidity Cyclops to a power source and set the Turbidity Cyclops to a gain setting see Setting the Gain section 3 2 for explanation on how to set the gain 2 Measure the
16. describe how to OO 1 Determine and set the appropriate gain Calibrate the Cyclops using standards with known concentrations Make measurements with the Cyclops Use the Solid Secondary Standard Note To make accurate and repeatable measurements it is important to keep the sensor clean see section 5 1 for information on cleaning your sensor 3 2 Setting the Gain Gain setting refers to the sensitivity configuration of the sensor There are three gain settings X1 X10 and X100 As the gain increases the sensitivity increases and the concentration range decreases 3 2 1 998 2100 Gain Determination Procedure 1 For in vivo applications take a natural sample of water from a sampling station where you plan to deploy the Cyclops Applying good measurement practices store it properly and quickly transport it to a laboratory where you have the Cyclops connected to a multi meter and DC power source see Figure 1 Pour the water sample into a clean glass beaker and submerge the optical end of the Cyclops see Appendix B for Recommended Measurement Practices for using your Cyclops in the Lab for how best to accomplish these steps Activate the X10 gain setting see Wiring Guide Appendix C if you believe the sample to represent a typical condition You would like to obtain a signal from the sample that is significantly higher than a blank sample de ionized water or filtered seawater but not a signal that is close t
17. e return shipment freight charges Address for Shipment Turner Designs Inc 845 W Maude Ave Sunnyvale CA 94085 998 2100 Rev 2 0 Page 13 Cyclops Submersible Sensor 998 2100 Rev 2 0 Page 14 Cyclops Submersible Sensor Appendix A Specifications Parameter Specification Linearity full range 0 99 R 3V Max 360 mW Input Voltage 3 15 VDC Signal Output 0 5 VDC Analog Temperature Range ADIER O e Water Temp 2 to 50 C Light Source Light Emitting Diode Visible Chl RWT PC PE F Excitation Wavelength UV CDOM fDOM Oil OB RF PTSA Turbidity R Detection Wavelength 300 1100 nm Standard 316 Stainless Steel Housing Material Cyclops 7 Optional Titanium Optional Plastic Stainless Steel and Titanium L 4 3 in 10 9 cm D 0 875 in 2 22 cm Plastic L 4 3 in 10 9 cm D 1 25 in 3 175 cm Dimensions Cyclops 6K L 6 6 in 6 76 cm D 1 75 in 4 45 cm Depth Rating Cyclops 7 600 meters Depth Rating Cyclops 6K 6000 meters Dimensions Cyclops 7 Weight Cyclops 7 5 oz 160 gm Weight Cyclops 6K 22 oz 620 gm 998 2100 Rev 2 0 Page 15 Cyclops Submersible Sensor Appendix B Recommended Measurement Practices Recommended Lab Practices for Measurements The following steps will improve the accuracy and repeatability of your measurements especially at low concentration levels 1 Use a non fluorescent container for your water samples Note
18. e diluted in deionized water http prozyme com 998 2100 Rev 2 0 Page 37
19. ecord the response as Weight 45 0 g 0 099 Ibs well as the gain values for that Length 5 7 cm 2 25 inches measurement Base Diameter 3 2 cm 1 25 inches A Solid Secondary Standard is not available for Turbidity Stainless or Titanium Cyclops 7 sensor 2 Dry off the optical end of the Cyclops 7 attach the Solid Secondary Standard to the fluorometer and adjust the Solid Secondary Standard to produce the same response in the same gain as in step 1 Use of the Solid Secondary 3 The Solid Secondary Standard s signal is Standard for in vivo Chlorophyll now equivalent to the concentration value Applications of the dye solution used in step 1and can be used in place of a liquid primary standard for future calibration of that 1 Using your Cyclops 7 Fluorometer specific Cyclops 7 measure a sample containing algae and record the response and the gain values Note Comprehensive information on for that measurement dye trace measurements can be found at the following Turner 2 Dryoffthe optical end of the Cyclops 7 Designs website attach the Solid Secondary Standard to http www turnerdesigns com flu the fluorometer and adjust the Solid orescent dye tracing Secondary Standard to produce the same response in the same gain as in step 1 3 Perform a chlorophyll extraction to Care and Storage determine the actual chlorophyll concentration of the sample Solid Secondary Standards should be stored at room temperature 20 C
20. eeded use optical tissue to clean the window with soapy water Note The Cyclops should NOT come in contact with any organic solvents i e acetone methanol or strong acids and bases The UV Cyclops models are the ONLY Cyclops sensors that can be calibrated with Quinine Sulfate standards made in Hydrosulfuric Acid All other Cyclops models CANNOT be used in Hydrosulfuric Acid 5 2 Warranty Terms Turner Designs warrants the Cyclops and accessories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the date of shipment from Turner Designs with the following restrictions e Turner Designs is not responsible for replacing parts damaged by accident or neglect Your instrument must be installed according to instructions in the User s Manual Damage from corrosion is not covered Damage caused by customer modification of the instrument is not covered e This warranty covers only Turner Designs products and is not extended to equipment used with our products We are not responsible for accidental or consequential damages except in those states where this limitation is not allowed This warranty gives you specific legal rights and you may have other rights which vary from state to state e Damage incurred in shipping is not covered 998 2100 Rev 2 0 Page 12 Cyclops Submersible Sensor 5 3 Warranty Service To obtain service during the warranty period the owner shall t
21. f the relay to the Cyclops gain control pin 998 2100 Rev 2 0 Page 20 Cyclops Submersible Sensor Appendix G Linear Range Quenching and Temperature Considerations The linear range is the concentration range in which the fluorometer s output is directly proportional to the concentration of the signal The linear range begins with the smallest detectable concentration and spans to an upper limit concentration that is dependent upon the properties of the material filters used and path length A non linear relationship is seen at very high concentrations where the signal does not increase at a constant rate in comparison to the change in concentration see figure below At even higher concentrations the signal will decrease even though the sample concentrations are continuing to increase This effect is Known as signal quenching Linearity can be checked by diluting a sample 1 1 or some other convenient ratio If the sample is still in the linear range the reading will decrease in direct proportion to the dilution If the reading does not decrease in direct proportion to the dilution or if the reading increases the sample is beyond the linear range Temperature Considerations Fluorometer Response Curve Sample Quenching Region Graph showing Linear and Quenching Regions of the sample s response Fluorescence is temperature sensitive As the temperature of the sample increases the fluorescence decreases
22. g clockwise hand tighten the air purge slot end of the Shade Cap onto the Cyclops 6K Sensor s optical head until the Shade Cap is fully secured to the unit Turning clockwise hand tighten the End Cap to the other end of the Shade Cap until it is fully secured to the Shade Cap Optional Completed Shade Cap Installation Specifications Delrin 50 0 g 0 11 Ibs 9 8 cm 3 86 in 4 4 cm 1 74 in Material Weight Length Diameter Depth Rating 6000 meters Rev 2 0 Page 25 Cyclops Submersible Sensor Appendix I Installing the Flowthrough Cap Introduction The Stainless or Titanium Cyclops 7 Flowthrough Cap P N 2100 600 was designed for use with in situ instrument packages that utilize a submersible pump for flow through sampling The Flowthrough Cap should not be subjected to a pressurized water source that will exceed 50 PSI of differential pressure When using the Flowthrough Cap best results will be obtained using the X1 or X10 Gain Ranges of the Cyclops 7 sensor The Cyclops 7 Flowthrough Cap consists of the following components 1eaFlowthrough Cap e 3ea Compression Gaskets 1 installed 2 spares e 3eaO Rinas 1 installed 2 spares Assembly Procedure 1 Lightly smear soapy water on the inside of the compression gasket 2 Slide the compression gasket over the optical end of the sensor with the step on the gasket towards the connection pins end of Cyclops
23. ge conductor size or greater i e Beldon No 8426 cable 2 The 0 5 volt Analog output is connected to a device i e Data Logger with an input impedance of 1 MegOhm or greater 3 The supply Voltage to the Cyclops is between 5 and 15 volts 998 2100 Rev 2 0 Page 19 Cyclops Submersible Sensor Appendix F Controlling the Gain The operating range can either be set to one of the 3 available ranges which will be referred to as Static Gain Control or it can be dynamically changed to achieve the optimum operating range referred to below as Dynamic Gain Control The first approach is applicable when using the Cyclops as a stand alone sensor The second approach is applicable when the Cyclops is integrated into a system with control capability Both methods are implemented by grounding driving Low the appropriate gain control pin The X10 and X100 gain control pins are normally in a High state if they are not connected to anything This means the Cyclops default is the X1 gain largest concentration range mode The Cyclops can be put into higher gain lower concentration range modes by connecting either the X10 or X100 pin but not both at the same time to ground Method 1 Static Gain Control Connect the X10 or X100 pin to the analog ground pin of the Cyclops pigtail connector See the Gain Switching Table in Appendix C to determine the required configuration for desired gain measurement range See Appendix E for Pigt
24. gly we are concerned with preserving the surroundings wherever our instruments are used and happy to work with customers by complying with the WEEE Directive to reduce the environmental impact resulting from the use of our products WEEE Return Process To arrange the return of an end of life product proceed as follows If you purchased your instrument through a Turner Designs Distributor please contact your local representative They will instruct you where to return the end of life product If you purchased your instrument directly from Turner Designs please contact Turner Designs Customer Service By Phone 1 408 212 4041 or Toll Free 877 316 8049 By Email Customer Service at support turnerdesigns com Turner Designs will provide a WEEE RMA Number a Shipping Account Number and a Ship to Address Package and ship the product back to Turner Designs The product will be dealt with per Turner Designs end of life recycling program in an environmentally friendly way Cyclops Submersible Sensor 1 Introduction 1 1 Description The Turner Designs Cyclops Submersible Sensor is an accurate single channel detector that can be used for many different applications It is designed for integration into multi parameter systems from which it receives power and delivers a voltage output proportional to the concentration of the fluorophore particle or compound of interest The Cyclops voltage output can be correlated to concentration
25. hodamine WT applications use high density tubing to prevent absorption of the dye into the tubing The Cyclops 7 should not come in contact with any organic solvents i e acetone methanol or strong acids and bases Page 27 Cyclops Submersible Sensor Introduction Installation Instructions The Flowthrough Cap P N 2100 608 for the 1 Turning clockwise fully hand tighten the enable flow through mode The Flowthrough Cyclops 7 Sensor Cap will allow the Plastic Cyclops 7 Sensor to be configured with other instruments used in flow through systems Features e Facilitates Flow through sampling e Eliminates other light sources that may interfere with sensor measurements e Protects sensors from damage during 2 Flowthrough Cap installation is complete deployment or transport Out Flow Port e Helps limit biofouling e Corrosion resistant plastic material NOTE For optimal use Turner Designs ms recommends Ss positioning or A mounting the Plastic UD Cyclops 7 Sensor Soe In Flow Port with Flowthrough Plastic Cyclops 7 Sensor Cap vertically This will purge any air from the system that might cause skewed readings e Threaded end for easy installation and removal Specifications Threaded ends M tal Fastener See Note Below Length w port 6 4 cm 2 52 in Note The Metal Fastener secures the ports Diameter w port 6 7 cm 2 64 in to the Flowthrough Cap s housing It sh
26. ibration setup and measurement Connecting the Cyclops to Turner Designs DataBank Simply connect the bare wires from the Cyclops s pigtail to the DataBank board as shown below and move the switch to select dV Switch should Bare wires connected Black Blue Brown Red Green 998 2100 Rev 2 0 Page 36 Cyclops Submersible Sensor Appendix L Optical Specification Guide ee ee A LED MDL Dynamic Range CWL E3 Ta XXXX 000 G Oil Fine 2ppb 010 000 ppb 285 nm lt 290 nm 350 55 nm 2100 904 905 2 ppm gt 100 ppm Application Excitation Emission Sol Std xxxx 000 B Optical Brighteners for d6 ppb 0 15 000ppb 365nm 325 120 nm 445 15 nm 2100 904 905 2160 901 Wastewater Treatment XXXX 000 P ee alla 2 ppb 0 40 000 ppb 590nm lt 595 nm gt 630 nm 2100 900 908 2160 900 Cyanobacteria Soave MSM a MINES SERRE Ox a 525nm 515 547nm 2590 nm 2100 900 908 2160 900 Cyanobacteria XXXX 000 R Rhodamine Dye 0 01 ppb 0 1000 0 4000 ppb 530 nm nm 530nm 535 60nm 535 60nm 590 715 nm 715 nm 2100 900908 2100 900908 XXXX 000 A PTSA 0 1 ppb 0 650 ppb 325 120 nm 405 10 nm 2100 904 905 1 5 Naphthalene Disulfonic Disodium Salt Quinine Sulfate PTSA 1 3 6 8 Pyrenetetrasulfonic Acid Tetrasodium Salt BTEX Benzene Toluene Ethylbenzene Xylenes Pe Phycocyanin pigment from Prozyme diluted in deionized water http prozyme com PE Phycoerythrin pigment from Prozym
27. ion resistant material e In line design maintains sensor s diameter e Large flow slots for improved flow Plastic Cyclops 7 Sensor N lt S A q p a a a 4 a L Flow Slots Air Purge Slots Installation Turning clockwise hand tighten the air purge slot end of the Shade Cap onto the Plastic Cyclops 7 Sensor s optical head until the Shade Cap is fully secured to the unit Turning clockwise hand tighten the End Cap to the other end of the Shade Cap until it is fully secured to the Shade Cap Optional Completed Shade Cap Installation Specifications Delrin 22 9 g 0 05 Ibs 9 2 cm 3 62 in 3 1 cm 1 22 in Material Weight Length Diameter Depth Rating 600 meters Page 24 998 2100 Rev 2 0 Cyclops Submersible Sensor Introduction The Cyclops 6K Shade Cap P N 2160 700 offers protection for the optics located at the optical end of the instrument It prevents damage from deploying recovering or transporting the instrument in fast flowing environments and or from bottoming out in shallow environments The Shade Cap also helps block other possible sources of light that may interfere with the Cyclops 6K Sensor s measurements Features Threaded ends for easy installation and removal Corrosion resistant material In line design maintains sensor s diameter Large flow slots Cyclops 6K Flow Slots End Cap 998 2100 Installation 1 Turnin
28. n Note You will feel hear a click when the Ball Plunger P N 2160 901 CDOM fDOM and Crude seats into position Oil Features e Can be used in place of a primary liquid standard once a correlation between a primary standard and the solid standard is established 3 The Solid Standard s signal is now ready to be adjusted using the green e Can be used to check fluorometer stability screwdriver provided with the Solid and or check for loss in sensitivity Standard e Provides a broad range of very stable fluorescent responses e Has an adjustment screw allowing users ee N to set to a desired signal Pa B Ball Plunger ee IX 4 Unscrew the locking nut as far as it will U go 5 Insert the green screwdriver through the hole in the locking nut and rotate until it engages with the adjustment screw on the Solid Standard Adjustable Filter beneath the locking nut Adjustable Filter i y ee 6 Rotate the Adjustable Filter to adjust the Locking reading Turning clockwise increases the Nut signal and counterclockwise decreases the signal 7 Once the desired reading has been ee i obtained the locking nut should be Specifications screwed down to hold the Adjustable Filter firmly in place l Note The response of every solid Material Delrin standard is unique A new correlation Weight 90 7 g 0 2 Ibs must be determined for every sensor Base Diameter 5 2 cm 2 05 inches
29. o the maximum of 5 Volts If the sample signal is high gt 3 0 V for example you may choose to use the X1 gain instead of the X10 gain setting so that you avoid going over scale once you deploy the Cyclops If the sample signal is very low lt 0 3V you may choose to use the X100 gain setting to achieve higher sensitivity but a smaller measurable range This process is easier for dye tracing applications Simply create the dye dilution of interest and record what signal level it provides on the three gain settings Rev 2 0 Page 8 Cyclops Submersible Sensor 3 2 2 Static Gain Control If integrating into a multi parameter system or data logger that is set up for Static Gain Control which refers to the use of only one gain setting at a time then you must determine which gain to use prior to deployment see section 3 2 1 and have an integration cable made to activate that specific gain see Appendix C For most applications the X10 gain will provide the best sensitivity range and resolution Customers wanting to dynamically change the gain ranges to achieve the optimum operating range should refer to Method 2 Dynamic Gain Control in Appendix F on how to interface with a Data Collection System with programmable outputs 3 2 3 Auto Gaining Certain data loggers or multi parameter systems will have an auto gaining feature which will automatically adjust the sensitivity according to the voltage output from the Cycl
30. ops sensor This feature maximizes the performance of Cyclops sensors allowing users to detect a broad range of concentrations obtain the best resolution and read minimum detection limits without having to rewire or manually change the sensor s sensitivity Turner Designs manufactures the DataBank Handheld Data Logger see Appendix K which has the auto gain feature and other functions that maximize the performance of Cyclops sensors 3 3 Direct Concentration Calibration Calibrating the Cyclops is a simple process requiring the use of calibration standards The Cyclops can be calibrated using a single calibration standard which correlates the standard s concentration to the voltage measured for that specific standard 1 Connect the Cyclops to a power source and set the Cyclops to a gain setting see section 3 2 1 for explanation on how to determine gain 2 Measure the voltage from a blank sample for the configured gain setting Note A good blank to use for this application is ultra pure or deionized water 3 Use a standard of known concentration and create a correlation between the standard s concentration and its voltage output 998 2100 Rev 2 0 Page 9 Cyclops Submersible Sensor 4 Once a correlation has been made use the following equation to calculate concentration values for sample measurements for the calibrated gain Csample E Csta VoltSsta VoltSpiank VoltSsample VoltSgiank Cs Concentration value of st
31. ops sensors are analog output devices that produce a 0 5 volt signal that is proportional to the fluorophore being measured Turner Designs offers the DataBank a universal handheld meter datalogger and power supply that can be used to maximize performance of Cyclops sensors with functions such as Auto gaining User defined calibrations Large internal memory Interval logging The DataBank comes with intuitive GUI software that allows users to easily calibrate set up logging download data and define parameters and values necessary to help configure the Cyclops for a specific application or study Available options include GPS capability external power travel case and car charger DataBank uses for different sampling protocols Multiple Site Measurements measure fluorescence at different locations within your water system or across many systems GPS enabled units provide latitude longitude data per location Profiling purchase extended cables to allow for vertical profiling Monitoring deploy the Cyclops to a fixed location or depth and monitor the signal over time set up logging to capture a signal within a specific time frame download data while the sensor is deployed and continuously measuring set up digital data output via HyperTerminal For any application or sampling protocol the DataBank facilitates and maximizes performance of all Cyclops sensors allowing versatility and flexibility in cal
32. ould Weight 92 2 g 0 203 lbs never be removed or tampered with Doing so may result in damage to the Flow Cap 9 00 ml ports and cause injury to yourself or others during operation 998 2100 Rev 2 0 Page 28 Cyclops Submersible Sensor Introduction The Flowthrough Cap P N 2160 600 for the Cyclops 6K is designed to enable flow through mode The Flowthrough Cap allows the Cyclops 6K Sensor to be configured with other instruments used in flow through systems Features e Facilitates Flow through sampling e Eliminates other light sources that may interfere with sensor measurements e Protects sensors from damage during deployment or transport e Helps limit biofouling e Corrosion resistant plastic material e Threaded end for easy installation and removal Cyclops 6K Sensor Ports a Threaded ends Metal Fastener See Note Below Note The Metal Fastener secures the ports to the Flowthrough Cap s housing It should never be removed or tampered with Doing so may result in damage to the Flow Cap ports and cause injury to yourself or others during operation 998 2100 Installation 1 Turning clockwise fully hand tighten the Flowthrough Cap housing onto the Cyclops 6K Sensor LT 4 4 4 LT r L1 k 1 1 f a Th Up ka Flowthrough Cap installation is complete NOTE For optimal use Turner Designs recommends positioning or mounting the Cyclops 6K Sensor with Flo
33. rd and the solid standard is established e Can be used to check fluorometer stability 3 and or check for loss in sensitivity e Provides a broad range of very stable fluorescent responses e Has an adjustment screw allowing users to set to a desired signal Ball Du an ANS 4 Filter Ny N 5 ANUS Solid Secondary LA amp Standard Locking N 6 Nut 7 Specifications Weight 65 9 g 0 145 lbs Length 8 2 cm 3 23 inches 3 6 cm 1 42 inches Solid Secondary Standard is not available for Turbidity Plastic Cyclops 7 sensor 998 2100 Rev 2 0 Plastic Cyclops 7 Sensor s optical head and snap on the Solid Standard Slightly rotate Solid Secondary Standard until it is set into position Note You will feel hear a click when the Ball Plunger seats into position The Solid Secondary Standard s signal is now ready to be adjusted using the green screwdriver provided m i 5 1 L a z a Fi ns 7 Unscrew the locking nut as far as it will go Insert the green screwdriver through the hole in the locking nut and rotate until it engages with the adjustment screw on the Adjustable Filter beneath the locking nut Rotate the Adjustable Filter to adjust the reading Turning clockwise increases the signal and counterclockwise decreases the signal Once the desired reading has been obtained the locking nut should be screwed down to hold the Adjustable Filter firmly in place Note The re
34. rd the response as well as the gain values for that measurement 2 Dry off the optical end of the Cyclops 7 attach the Solid Secondary Standard to the fluorometer and adjust the Solid Secondary Standard to produce the same response in the same gain as in step 1 998 2100 Rev 2 0 3 The Solid Secondary Standard s signal is now equivalent to the concentration value of the dye solution used in step 1and can be used in place of a liquid primary standard for future calibration of that specific Cyclops 7 Note Comprehensive information on dye trace measurements can be found at the following Turner Designs website http www turnerdesigns com flu orescent dye tracing Care and Storage Solid Secondary Standards should be stored at room temperature 20 C in their case when not in use and kept free of dust and moisture Special care must be taken with the UV Solid Secondary Standard P N 2100 905 to ensure that it is not exposed to UV light for prolonged periods of time This can result in degradation of the standard Page 33 Cyclops Submersible Sensor Introduction Installation The following information describes how to use the Cyclops 6K Solid Secondary Standards 1 Align Solid Secondary Standard with Cyclops 6K Sensor s optical head and P N 2160 900 In Vivo Chlorophyll snap on the Solid Secondary Standard Phycocyanin and 2 Slightly rotate Solid Secondary Standard Phycoerythrin until it is set into positio
35. sponse of every solid secondary standard is unique A new correlation must be determined for every sensor Page 32 T A a Cyclops Submersible Sensor Use of the Solid Secondary Standard for in vivo Chlorophyll Applications 1 Using your Cyclops 7 Fluorometer measure a sample containing algae and record the response and the gain values for that measurement 2 Dry off the optical end of the Cyclops 7 attach the Solid Secondary Standard to the fluorometer and adjust the Solid Secondary Standard to produce the same response in the same gain as in step 1 3 Perform a chlorophyll extraction to determine the actual chlorophyll concentration of the sample Note EPA Method 445 0 in vitro determination of chlorophyll in algae can be found on Turner Designs website 4 The Solid Secondary Standard s signal is now equivalent to the concentration value determined from step 3 and can be used in place of a liquid primary standard for future calibration of that specific Cyclops 7 Fluorometer Use of the Solid Secondary Standard for Dye Tracing Applications The Solid Secondary Standard can also be used to check fluorometer stability when making dye concentration measurements If necessary the Solid Secondary Standard can be used to establish a new correlation voltage without using a calibration solution each time 1 Using your Cyclops 7 Fluorometer measure a dye solution with known concentration and reco
36. t fluorescent responses Adjustment screw is located under the locking nut e Has an adjustment screw allowing users A to set to a desired signal y 7 A Insert the supplied green screwdriver through the hole in Installation J the locking nut to reach the adjustment screw 1 Before installing the Solid Secondary Standard ensure that the optical surface 6 Once the desired reading is obtained of the Cyclops 7 is completely clean and tighten the locking nut so the adjustment dry screw is held firmly in place 2 Fully mate the Solid Secondary Standard 7 Finish by noting the output voltage and with the optical end of the Cyclops 7 gain setting used X1 X10 or X100 in the Value space on the Secondary Standard 3 Rotate the Solid Secondary Standard in label either direction until you feel the indexing ball click into the indexed position 8 Note that the response of every Solid ee Secondary Standard is unique Hence a Solid Standard new correlation must be determined for indexing mark every sensor For future identification use the ID space on the label for a unique identifier for the Secondary Standard Solid Standard indexing ball Align the index mark and indexing ball when mating the Cyclops 7 and the Solid Secondary Standard 998 2100 Rev 2 0 Page 30 Cyclops Submersible Sensor Specifications 1 Using your Cyclops 7 Fluorometer measure a dye solution with known Material concentration and r
37. wthrough Cap vertically This will purge any air from the system that might cause skewed readings Specifications Rev 2 0 Out Flow Port B A a T a ea A m z p A Aie Diameter w port 7 0 cm 2 77 in Weight 86 2 g 0 19 Ibs In Flow Port Length w port 7 1 cm 2 81 in Page 29 Cyclops Submersible Sensor Appendix J Installing and Using the Solid Secondary Standard Introduction 4 Use a flathead screwdriver to unscrew the The following information describes how to use locking nut as far as it will go the Stainless or Titanium Cyclops 7 Solid Secondary Standards 5 Tochange the signal level use the green screwdriver provided and insert the blade P N 2100 900 In Vivo Chlorophyll through the hole in the locking nut Rotate Rhodamine WT the screwdriver until it engages with the Fluorescein Phycocyanin adjustment screw that is beneath the and Phycoerythrin locking nut Rotate the screw to adjust the signal level as necessary Turning P N 2100 904 CDOM DOM Crude Oil clockwise increases the signal and Refined Fuels and Optical counterclockwise decreases the signal Brighteners Features e Can be used in place of a primary liquid standard once a correlation between a primary standard and the solid standard is established e Can be used to check fluorometer stability and or check for loss in sensitivity e Provides a broad range of very stable Lock nu
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