Rat IL-10 Singleplex Bead Kit Technical Data Sheet
Contents
1. invit rogen Rat IL 10 Singleplex Bead Kit Technical Data Sheet Catalog LRC0101 Lot 366555 Note A letter at the end of the lot number signifies an additional packaging of this same lot Intended Use This kit is comprised of components for the measurement of rat IL 10 in serum plasma or tissue culture supernatant The assay may be run alone or in combination with other Singleplex Bead Kits from Invitrogen Buffer reagents needed to complete the reaction are sold separately under Catalog LMB0001 These reagents are intended for use in the Luminex 100 or 200 System only This kit is configured for research use only and is not to be used in diagnostic procedures Reagents Provided Rt IL 10 1 Part LM149 Description Antibody Bead Concentrate 10X Lot 403249 Size 0 25 mL 100 tests Bead Region 15 Form 0 25 mL 10X bead concentrate solution in storage buffer Contains 0 05 sodium azide as a preservative Storage Light sensitive material Store at 2 to 8 C in the dark until the expiration date indicated on the kit Rt IL 10 2 Part BN149 Description Biotinylated Ab Conc 10X Lot 494724 Size 1 mL 100 tests Form 1 mL of a 10X stock of Biotinylated Antibody Concentrate in Biotin Diluent Contains 0 1 sodium azide as preservative Concentration of antibody is matched to this lot of beads Do not mix lots of Coated Beads and Detection Antibody Storage Store at 2 to 8 C until the expiration date indicated on t2. IFU accompanying the product By purchasing this Kit which contains fluorescently labeled microsphere beads authorized by Luminex Corporation Luminex you the customer acquire the right under Luminex s patent rights to use this Kit or any portion of this Kit including without limitation the microsphere beads contained herein only with Luminex s laser based fluorescent analytical test instrumentation marketed under the name Luminex 100 or 200 This product is covered by one or more of the following U S patents 6 046 807 For research use only CAUTION Not intended for human or animal therapeutic or diagnostic use www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo e CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PiLRCO101 Rev 01 10 Page 2 of 2 DCC 10 0210 Important Licensing Information These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or our website www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses Unless otherwise indicated these products are for research use only and are not intended for human or animal diagnostic therapeutic or commercial use
3. ces at 10 ng mL were assayed with the Invitrogen Rat IL 10 Singleplex Bead Kit The following substances were tested and all were found to have no cross reactivity rat IL la IL 1B IL 2 IL 4 IL 5 IL 6 IL 12 GM CSF IFN y TNF a MCP 1 MIP la VEGF mouse IL 10 and human IL 10 Precision Intra assay Inter assay n 16 n 32 Mean pg mL 4605 4 4762 4 SD 119 7 226 6 CV 2 6 4 8 Linearity Rat serum and tissue culture medium containing 10 fetal calf serum were spiked with rat IL 10 and serially diluted in Assay Diluent and a solution consisting of 50 Assay Diluent and 50 tissue culture medium containing 10 fetal calf serum respectively over the range of the assay Linear regression analysis of samples versus the expected concentration yielded a correlation coefficient of 0 99 for serum and tissue culture Recovery Rat serum averaged 95 4 range 85 4 to 100 7 Rat EDTA plasma averaged 95 0 range 90 to 99 4 Heparin plasma averaged 89 6 range 84 4 to 97 9 Citrate plasma averaged 95 3 range 93 4 to 97 6 Tissue culture medium containing 10 fetal calf serum averaged 83 6 range 66 7 to 102 8 Explanation of n a E Research Use Only In vitro In vitro diagnostic medical device medical device ai European Community authorised representative D without does not contain With contains Protect from light Consult accompanying documents A Dires the user to consult instructions for use
4. he kit 3 Part SM0562 Description Rt Cytokine 10 Plex Standard Lot 491428 Size 2 Vials Form Lyophilized The proteins in this standard have been calibrated against the masses of highly purified recombinant proteins with the respective Invitrogen ELISA kit Refer to the User Manual included in the Buffer Reagent Kit for further information Contains 0 1 sodium azide as a preservative Storage Store at 2 to 8 C Use within hour after reconstitution Discard immediately after use Concentration of Reconstituted Standards GM CSF 5 320 pg mL IFN y 4 350 pg mL IL 1a 31 660 pg mL IL 1 16 840 pg mL IL 2 34 020 pg mL IL 4 2 040 pg mL IL 6 8 300 pg mL IL 10 36 300 pg mL IL 12 10 390 pg mL TNF a 1 260 pg mL Tmportant note The concentrations of reconstituted standard are lot specific Please verify all concentration values entered in data analysis software Reconstitution Reconstitute with 1 0 mL Assay Diluent when measuring IL 10 in serum or plasma samples For other sample types such as tissue culture supernatants reconstitute the standard in 1 0 mL of a solution consisting of 50 Assay Diluent 50 sample matrix Allow standard to rehydrate for approximately 10 minutes before further dilution Recommended Starting Concentration for Standard Curve Upon reconstitution the starting concentration of standard is the value cited above Make serial 1 3 dilutions in Assay Diluent serum plasma samples or other approp
5. riate matrix Use 100 uL per assay If establishing a Multiplex Assay this same standard can be used to measure the other related cytokines cited above in a Multiplex Assay format Refer to the User Manual included in the Buffer Reagent Kit for further information For research use only CAUTION Not intended for human or animal therapeutic or diagnostic use www invitrogen com Invitrogen Corporation 542 Flynn Rd Camarillo e CA 93012 e Tel 800 955 6288 E mail techsupport invitrogen com PiLRCO101 Rev 01 10 Page 1 of 2 DCC 10 0210 Important Licensing Information These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or our website www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses Unless otherwise indicated these products are for research use only and are not intended for human or animal diagnostic therapeutic or commercial use Rat IL 10 Singleplex Bead Kit Technical Data Sheet Performance Characteristics Analytical Sensitivity The minimum detectable dose of rat IL 10 is lt 30 pg mL This was determined by adding two standard deviations to the mean FI obtained when the zero standard was assayed 30 times Typical Standard Curve Pet IL 10 10000 2 100 S 100 9 S 10 LL 1 I 1 10 100 1000 10000 IL 10 pgri Specificity Buffered solutions of a panel of substan
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