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        Prior Automatic Plate Loader Manual
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1.  100v to 240v      Fuse rating for both fuses is T2A      Communication Via USB    PLW20 5    Section 3 Unpacking The System    3 1 Unpacking The System    6 Prior Scientific    Section 4 Installation    4 1 Installing Your System    Remove X axis assembly from packaging case and place on suitable bench        Remove Y axis tower assembly from packaging and lower onto x axis assembly as  shown  Ensure mating faces are debris free and aligned correctly        Insert M5 x 16 socket       button head screw  into each of four       h E  mil ia  9    Anti rotation  shibbina  Insert M3 x 6 socket  PL W20 nan head screw to 7    With Y axis tower installed  remove anti rotation shipping screw   Remove well plate racks from packaging and fit onto loader plate   Ensure locking clasp is positioned as shown below     ___  Plate        TE    is  br     Well plate  rack locking    Locate dovetail on front of rack under plate dovetail on loader and lower rear of  rack down onto plate        With rack in position move clasp to right to secure rack in place and activate    rack  present switch        Repeat for rack 2     8 Prior Scientific    Rear view of loader shows cable entry points   Mains socket  To supply 4 way binder from well plate on stage sensor   USB to PC              gt       gt           he               gt      PLW20    1  Connection    1 1   1 2     1 3     Connect the Well Plate Loader to the PC with a standard USB cable     Connect Well Plate Stage to a Proscan III control
2.  Oster HirumMowez Stage Helo    Walpana Mora Sytem Sua    Mosa Salam Saus                     Hote  2   Hotel 1 3         Stage Hotel 2 Hae 1          Pause     Para                      dirai    Do Single Sen Ds Erie 7  Scan   Scan   Ecm       AA AAA                 4 5  When the scan has completed  the well plate should be indicated by a  green bar in the    Hotel 1   column     4 6  Click    Debug    gt  Single Step Enable        Screenshot  Debug gt Single Step Enable       4 7  Start transfer of the well plate to the stage by Clicking on the green  well plate indicator  the cursor icon will change  and then clicking on  the stage indicator     Single step the operation until the plate is completely clear of the hotel     Prior Scientific    Es    Prior Wellplate Loader      ee oen  Debug Opis Marlia Slag Hap                                                Continue      ho E MEZ    Connect SoakTest Debug Options Manual Move 5     Wellplate Move  CONNECTED  Stage    Hotel 1            IDLE          lt        m          Pause         11111111                    6 8  Check the setup position of the well plate in the gripper  The bottom of  the plate should be flush with the bottom of the gripper paddles     Bottom of well plate must be flush with  bottom of gripper paddles    6 9  Click    Pause     then    Stop      6 10  Click    Debug    and uncheck    Single Step Mode        6 11  On the menu bar click    Stage    gt  Stage to load     The stage will move to  the loading 
3. 10    Prior Scientific    After initialisation has completed and if setup has note been done  then the manual mover screen  will re appear     E or Wellplate Loade       Connect SoakTest Debug Options T Stage Help    m   Screenshot of Manual Move  a la    a 3           interface          0                 CONNECTED 0    3 1  Use the manual move buttons to step the gripper to    1  reference point  Check that the gripper arms are central in the insert   flush with the base  and the leading edges of the gripper paddles are  flush with the tabs        Leading edges of gripper paddles  must be flush with tabs        3 2  Click    Store Hotel 1 Position    on the setup     Screenshot  Store Hotel 1 position Screenshot  Store Stage Position       3 3  Using the manual move buttons drive the gripper arm to a safe point  above the stage     3 4  Click    Store Stage Position    to save this temporary safe position   3 5  Click    Save and Exit     The Manual Move setup screen will close     3 6  On the menu bar click    Disconnect        PLW20 10    LE    Screenshot  Save and Exit Screenshot  Disconnect       4  Setup     Part 2  4 1  On the menu bar click    Connect      4 2  Allow the loader to initialise     4 3  Place a hotel  well plate cassette  containing one well plate in the     Hotel 1    position on the kidney plate     4 4  Click the    Scan    button below the    Hotel 1    column     RETTE      Connect ScakTest Debug Options  Manualllove Sie He       Connect Saket Debug
4. PLW20       Prior Automatic Plate Loader  Manual    Version 1 0    PLW20    Table of Contents    Contents   Section  SAFETY INFORMATION  Section 2 SPECIFICATIONS   Section 3 UNPACKING THE SYSTEM  Section 4 INSTALLATION   Section 5 ROUTINE MAINTENANCE  Section 6 ADVANCED OPERATION  Section 7 TROUBLESHOOTING  Section 8 REPLACEMENT PARTS  Section 9 RETURNS  amp  REPAIRS  Section 10 APPENDICES    7 Prior Scientific    Section   Safety Information    1 1 Important Safety Information    A SAFETY WARNINGS    Always observe the following safety precautions    e The PL200 must only be connected to a power outlet  which provides a  protective earth     e Use only the mains cord supplied  If this does not fit your power outlet   contact your distributor for the correct power lead     e The unit is heavy and weighs 37kg  Remove the microscope before  transporting  It should only be lifted by two people  use side handle and lip  of the rear cable entry area to lift safely     e Always disconnect the equipment before moving     e This equipment is for use in moderate climates only  NEVER use the  equipment in damp or wet conditions     e Avoid excessive heat  humidity  dust  amp  vibration     e Do not use where the equipment may be subjected to dripping or splashing  liquids     e This equipment contains no user serviceable parts  Refer all repairs to  qualified service personnel     e Keep fingers and hands clear of the PL200 moving parts when it is operating     e Itis the user operator
5. ler using the Prior  Stage cable     After checking that the Well Plate Loader and Proscan III controller  switched off  connect them to the mains supply     2  Initialisation    2 1   22  PESA    2 4        Remove any hotels  well plate cassettes  from the loader   If possible  manually move the loader into a clear  unrestricted position     Make sure that nothing is in a position where the loader can collide  with it     Switch on the Well Plate Loader     Open Prior Well Plate Loader software     polini xi           Prior Wellplate Loader    SoakTest Debug Options Manual Move Stage Help          i    Wellplate Move                                    i M System Status  m Stage Hotel 2       Hotel 1        ERROR    fe   NOT CONNECTED    i  ETE                                     m_T_            _         _      m   __   __   __________ ti    2 6     2 7    2 8     On the menu bar  click    Connect     then select or confirm the Comm  port number     Enter or confirm the Proscan Ill Comm port number     The manual mover screen will appear  use this to move the loader to a position clear of the  stage and about half way up the Y axis     A warning may appear indicating that a well plate is in the gripper  Manually remove this  well plate and continue  Use the G button in the manual mover screen to open the gripper     Closing the manual move screen will result in the stage being moved to its far left position  and then the robot will auto initialise     3  Setup     Part 1    
6. nty or otherwise  a Return Material Authorisation  RMA  number must be  obtained from the appropriate Prior Scientific office before returning any equipment  For  North and South America contact Prior Scientific Inc  and for the rest of the world call Prior  Scientific Instruments Limited on the telephone numbers shown below     Prior Scientific Instruments Ltd   Unit 4    Wilbraham Road    Fulbourn    Cambridge    ENGLAND    CB1 5ET   Tel  01223 881711   Fax  01223 881710    email  uksales prior com    Prior Scientific KK  Kayabacho 3  Nagaoka Bldg  10F  2 7 10   Nihonbashi Kayabacho   Chuo Ku   Tokyo   103 0025   JAPAN   Tel   81  0  3 5652 8831  Fax   81  0  3 5652 8832  email  info japan prior com    PLW20    Prior Scientific Inc    80 Reservoir Park Drive   Rockland    MA 02370 1062   USA   Tel  781 878 8442   Fax  781 878 8736    email  info prior com    Prior Scientific GmbH  Wildenbruchstr  15  D 07745   Jena   GERMANY   Tel   49  0 3641 675 650  Fax   44  0 3641 675 651    email  jena prior com    19    Section 10 Appendices    20 Prior Scientific    
7. position     6 12  On the menu bar  click    Options    gt  Redo Setup                                          E Prior Wellplate Loader E   xf           Prior Wellplate Loader    54 x   l Connect SoakTest Debug Options Manual Move i   Connect SoakTest Debug f Options   Manual Move Stage Help     Welplate Move     System Status     Wellplate Move System Status     Stage Hotel 2     Hotel 1 o  Stage Hotel 2     Hotel 1   0 ERROR 00 ERROR     NOT CONNECTED su NOT CONNECTED   i arme TATA ram f i arme Taree AT rum             6 13  Move the stage switch to the open position     6 14  Use the buttons on the manual move interface to transfer the well  plate to the stage insert and adjust the microscope base until the well  plate bears against the tabs on the back and left hand faces of the  stage insert Adjust the Y height so that the well plate is sitting on the  insert face     6 15     PLW20 Lo    14      000000000000      I 000000000000 Well plate must bear against    000000000000 2 tabs on back and left hand  ve faces of the stage insert       6 16  Click    Store Stage Position        6 17  Click    Save and Exit     Click    Disconnect        7  References    HLW20 Well Plate Loader User Manual    Prior Scientific    Section 5 Routine Maintenance    SAFETY   MAINTENANCE  Clean with a clean  slightly dampened cloth  Do not wet or allow moisture to  penetrate the unit     Do not use solvents on the slide loader as these may stain or damage the plastic   covers   A To ensure co
8. rrect slide handing it is important that the interface   between the slide racks and the slide rack mounting face is kept clean   and free of debris    Remove dust and debris from the slide rack mounting plate using a brush    If the slide racks should become soiled with slide mounting media they can be   cleaned using your laboratories standard cleaning procedure     The PLW20 only contains primary circuits with EMC filters  If PAT testing  apply DC  flash tests only  Avoid repeated application of such tests as this may damage  insulation     SERVICING  only to be undertaken by a competent person    This equipment contains no user serviceable parts  Refer all repairs to qualified  service personnel    Opening the product voids the warranty     Oil and residue removal   Rinse the slide racks in your laboratories designated cleaning solvent to remove the  oll    Refer to your health and safety procedures on using this solvent      PLW20 ES    Section 6 Advanced Operation    Software Integration     The information required for software integration is included in the software  development kit  This can be downloaded from the Prior Scientific website at    www prior com     16 Prior Scientific    Section 7    PLW20    Troubleshooting    17    Section 8 Replacement Parts    Replacement Parts    18 Prior Scientific    Section 9    Returns and Repairs    Returns and Repairs    Should you experience problems with your Plate Loading System and want to send it back  for service  warra
9. s responsibility to ensure good laboratory practise Is  followed if slides contain hazardous material     PLW20 3    SAFETY SYMBOLS    Retain these instructions  The following symbols mean     Warning  read instructions to understand possible hazard    A Warning  hazardous voltage       GENERAL GUIDELINES FOR USE OPERATION    The PLW20 forms part of an automated microscopy system  which may be used in  research or production laboratories  It will pick up  present  sort and return plates  to any of the two preloaded racks that are positioned at the front     Whilst operating if a plate becomes misaligned  do not attempt to correct this  during the operation cycle  Turn off the mains power before correcting plate    position or removing any obstruction  Reinitialise the system     The PLW20 rotary arm may move unexpectedly  under automatic control     4 Prior Scientific    Section 2 Specification    2 1 Specifications    This equipment is for indoor use and will meet its performance figures within an ambient  temperature range 5 to 40  C with maximum relative humidity of 80  humidity   deteriorating linearly to 50  above 312C             Weight 57Kg gross   includes Slide loader  all accessories and packaging   37Kg Net   plate loader fully assembled with accessories  but no packaging   The weight of the    microscope is not included in these figures       Capacity of 20 slides  2 racks of 10     type E A                   Plate change time of 25 seconds           Input Voltage
    
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