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R/I-1TM Manual - NPI Electronic Instruments

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1. D Electronic E Instruments for the Life Sciences Moasus lt e ade OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE R I T1M ELECTRODE RESISTANCE TEST CURRENT INJECTION MODULE FOR EPMS SYSTEMS um R I TIM pi um VERSION 1 2 npi 2014 npi electronic GmbH Bauhofring 16 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 support npielectronic com http www npielectronic com R I T1M User Manual Table of Contents 1 Safety Regulations iia redada escalas distan dededats A E 3 2 EPMS 07 Modular Plug In Md da ciao 4 2 1 General System Description Operation pili ida 4 Zoe A a O a O 4 2 3 EPMS E 07 HUSIN iia 4 DAs PWR O3D sida pas 4 DI System UA it od ee 5 205 Technical Dit A A lean Wetee 5 3 R I T1M Resistance Test Current Injection Module ooooocnnocccnonccconcccnoncnononcnonnncnonnnaninnnos 6 Frequency and Amplitude of Current Injection Pulse ooooonnccnnccinncnionononcnancnonanonnonos 8 AA O A tees Be ee ae ae 8 version 1 2 page 2 R I T1M User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic are NOT intended for clinical use or medical purposes e g for diagnosis or treatment of humans or for any other life supporting system npi electronic disclaims any warranties for such purpose Equipment supplied by npi electronic must be operated only by selected trained and adequately instructed personnel For det
2. ae or ay 00 C E x10 Fx20V dt zz ss 660 100 nA j because of having a biphasic pulses this has to be multiplied by 2 to get the frequency 660 us 2 1 32 ms gt 758 Hz 4 Technical Data R I T1M Resistance measurement 0 01 10 MQ display XX XX MQ Current injection 0 280 nA symmetrical around baseline display XXX nA Output scaling 1V MQ or 1 V 100 nA Output resistance 50 Q range 10 V version 1 2 page 8
3. loops the internal ground is isolated from the protective earth The internal ground is used on the BNC connectors or GROUND plugs of the modules that are inserted into the EPMS 07 housing The internal ground and mains ground protective earth can be connected by a wire using the ground plugs on the rear panel of the instrument It is not possible to predict whether measurements will be less or more noisy with the internal ground and mains ground connected We recommend that you try both arrangements to determine the best configuration a ZPMS E 07 The 19 cabinet is connected to the PROTECTIVE EARTH connector at the rear panel The chassis is linked to protective earth only if the PWR 03D is connected It can be connected also to the SYSTEM GROUND SIGNAL GROUND on the rear panel of the instrument see Figure 3 Important Always adhere to the appropriate safety measures Figure 3 Rear panel connectors of the EPMS E 07 2 6 Technical Data 19 rackmount cabinet for up to 7 plug in units Dimensions 3U high 1U 1 3 4 44 45 mm 254 mm deep EPMS 07 Power supply 115 230 V AC 60 50 Hz fuse 2 A 1 A slow 45 60 W EPMS E 07 External power supply for EPMS E 115 230 V AC 60 50 Hz fuse 1 6 0 8 A slow Dimensions of External power supply W x Dx H 225 mm x 210 mm x 85 mm version 1 2 page 5 R I T1M User Manual 3 R I T1M Resistance Test Current Injection Module The R I T1M module allows measurement
4. of the resistance of an electrode connected to an extracellular amplifier in the same EPMS housing The module can function also for injection of current up to 280 nA through a single electrode or through all electrodes simultaneously The function is selected by a switch and indicated by LEDs It can be operated manually or remotely using a TTL signal The two functions can be applied to up to six electrodes The resistance is determined by application of a fixed pulse of approx 10 nA with 200 Hz The current injection pulses are variable in both frequency and amplitude and symmetric around the baseline Test and current injection pulses are a triangle shaped signal fed into the headstage of the EXT amplifier With the capacitor in the headstage the triangle is differentiated resulting in a rectangular current signal at the electrode For electrode resistance test the voltage drop at the electrode caused by the current signal is measured and the electrode resistance is calculated due to Ohm s law I R C Frequency and amplitude of current injection are dependent on each other according to Ic C dU dt where Ic is the capacitive current C is the capacitor in the amplifier headstage dU dt is the change of the voltage amplitude of the triangle over time O R I TIM RESISTANCE CURRENT do Dr i 4 Figure 4 R I T1M front panel view version 1 2 page 6 R I T1M User Manual In the following description of the front panel
5. Housing The following items are shipped with the EPMS E 07 housing EPMS E 07 cabinet External Power supply PWR 03D Power cord PWR 03D to EPMS E 07 Mains chord Fuse 1 6 A 0 8 A slow Front covers KKK KK The EPMS E 07 housing is designed for low noise operation especially for extracellular and multi channel amplifiers with plugged in filters It operates with an external power supply to minimize distortions of the signals caused by the power supply 2 4 PWR 03D The external power supply PWR 03D is capable of driving up to 3 EPMS E housings Each housing is connected by a 6 pole cable from the one of the three connectors on the front panel of the PWR 03D to the rear panel of the respective EPMS E housing see Figure 1 Figure 3 A POWER LED indicates that the PWR 03D is powered on see Figure 1 Power switch voltage selector and fuse are located at the rear panel see Figure 2 Note The chassis of the PWR 03D is connected to protective earth and it provides protective earth to the EPMS E housing if connected version 1 2 page 4 R I T1M User Manual PWR O3D npi ON O O 0 e 08 j n E Figure 1 PWR 03D front panel view Figure 2 PWR 03D rear panel view Note This power supply is intended to be used with npi EPMS E systems only 2 5 System Grounding EPMS 07 The 19 cabinet is grounded by the power cable through the ground pin of the mains connector protective earth In order to avoid ground
6. ails please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic D 71732 Tamm Germany 1 2 3 4 5 GENERAL This system is designed for use in scientific laboratories and must be operated by trained staff only General safety regulations for operating electrical devices should be followed AC MAINS CONNECTION While working with the npi systems always adhere to the appropriate safety measures for handling electronic devices Before using any device please read manuals and instructions carefully The device is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the instrument when replacing the fuse or changing line voltage Replace fuse only with an appropriate specified type STATIC ELECTRICITY Electronic equipment is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic charge and must therefore be handled with care Electrostatic discharge can be avoided by touching a grounded metal surface when changing or adjusting sensors Always turn power off when adding or removing modules connecting or disconnecting sensors headstages or other com
7. elements each element has a number that is related to that in Figure 4 The number is followed by the name in uppercase letters written on the front panel and the type of the element in lowercase letters Then a short description of the element is given 1 RESISTANCE CURRENT switch Switch for selecting electrode RESISTANCE test or CURRENT injection The selected function is indicated by 11 or 2 respectively 2 CURRENT nA LED LED showing that CURRENT injection is selected The amplitude of the injected CURRENT is shown in display 3 in nA 3 RESISTANCE CURRENT display Digital display showing the tetrode unit resistance XX XX MQ range 0 01 MQ 10 MQ or the amplitude of the injected current XXX nA range 0 280 nA 4 CHANNEL SELECT indicator LED XX indicating the selected CHANNEL for current injection or to be tested for resistance The indicator starts at 01 e g 02 indicates function at CHANNEL 2 see also 10 5 AMPLITUDE potentiometer 10 turn Potentiometer for setting the current amplitude range 0 280 nA 6 OUT connector BNC connector monitoring the amplitude of the injected current 1 V 100 nA or the resistance 1 V 1 MQ 7 TTL IN connector BNC connector for remote control of operation with a TTL signal As long as the signal is HI gt 2 5V operation takes place 8 ON push button Push button for activating current injection or resistance test 9 FREQUENCY pote
8. ntiometer 10 turn Potentiometer for setting frequency of the current injection pulses range 200 Hz 2 kHz Important Changing the frequency will also change the amplitude of the current pulse 10 CHANNEL SELECT switch Switch for selecting the electrode for current injection to be tested for resistance Starting at electrode 1 CHANNEL 01 indicated by 4 turning clockwise increases the electrode number until electrode 6 Turning clockwise further selects all electrodes simultaneously for current injection This function is indicated by 4 showing CHANNEL 7 Note that only one digit is shown version 1 2 page 7 R I T1M User Manual Important In position CHANNEL 7 the electrode resistance test is disabled Only current injection will function 11 RESISTANCE MQ LED LED showing that electrode RESISTANCE test is selected The measured electrode RESISTANCE is shown in display 3 in MQ With electrodes gt 2 MQ the resistance is shown accurately only if the capacity has been compensated correctly with EXT 10C Frequency and Amplitude of Current Injection Pulse Frequency and amplitude of current injection are dependent on each other according to I Cx du dt where I is the capacitive current C is the capacitor in the amplifier headstage 3 3 pF dU dt is the change of the voltage amplitude of the triangle over time Example for frequency with fixed amplitude of the triangle 10 V i e 20 V amplitude dU fOr
9. ponents from the instrument or 19 cabinet TEMPERATURE DRIFT WARM UP TIME All analog electronic systems are sensitive to temperature changes Therefore all electronic instruments containing analog circuits should be used only in a warmed up condition i e after internal temperature has reached steady state values In most cases a warm up period of 20 30 minutes is sufficient HANDLING Please protect the device from moisture heat radiation and corrosive chemicals version 1 2 page 3 R I T1M User Manual 2 EPMS 07 Modular Plug In System 2 1 General System Description Operation 1 The npi EPMS 07 is a modular system for processing of bioelectrical signals in electrophysiology The system is housed in a 19 rackmount cabinet 3U has room for up to 7 plug in units The plug in units are connected to power by a bus at the rear panel 2 The plug in units must be kept in position by four screws M 2 5 x 10 The screws are important not only for mechanical stability but also for proper electrical connection to the system housing Free area must be protected with covers 2 2 EPMS 07 Housing The following items are shipped with the EPMS 07 housing Y EPMS 07 cabinet with built in power supply Y Mains cord Y Fuse2A 1A slow Y Front covers 3 In order to avoid induction of electromagnetic noise the power supply unit the power switch and the fuse are located at the rear of the housing 2 3 EPMS E 07

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