User Manual
Contents
1. ES The sample tray can move unexpectedly The system check and heating of the heating blocks will take 3 5 minutes depending on available voltage The main screen is displayed when the system check is finished 5 1 2 Preparing the Maintainor card Figure 7 Maintainor card 1 Prepare the Maintainor card by writing the sample ID and card ID at 1 shown in Figure 7 26 Stabilizor T1 DPM 0008 Edition 3 Heat stabilizing tissue samples 5 5 1 3 Preparing the Instrument Enter settings for the run according to Section 4 2 1 5 2 SAMPLE COLLECTION AND HEAT STABILIZATION 5 2 1 Sample collection 1 Open the Maintainor card fully one time to avoid unwanted closing of the card when loading the sample Place the card depending on the sample type y mp e Fresh sample Place the Maintainor card directly in the Stabilizor T1 before collecting the sample This will give the shortest ex vivo time e Frozen sample Place the Maintainor card on ice to keep the sample cold before the stabilization Caution Do not put the Maintainor card into liquid nitrogen The Maintainor card withstands temperatures down to 80 C 2 Start the measurement of the ex vivo time just prior to sampling by pressing the ex vivo button on the screen and immediately continue with step 3 3 Collect a sample of appropriate size Stabilizor T1 DPM 0008 Edition 3 27 denator 4 Putthe sample in the center of the sample chamber of the Maintainor2. Stabilizor T1 DPM 0008 Edition 3 denator 5 1 3 Preparing the Instrument 28 5 2 SAMPLE COLLECTION AND HEAT STABILIZATION 28 5 2 1 Sample collection n nanna aaan aaa 28 5 2 2 Sample stabilization 29 5 3 LOGFILES LL 30 5 4 EXTRACTION OF PROTEINS FROM HEAT STABILIZED TISSUE SAMPLES 30 Maintenance 00 cee eee eee 32 6 1 MAINTENANCE AND SERVICE 32 6 2 CHANGING THE O RING 0 00000 e eee 32 6 3 CHANGING THE NEEDLE DEVICE 35 6 4 REPLACE THE BATTERY ee eee 38 6 5 CLEANING THE INSTRUMENT 00 39 DIAGNOSTICA init duced ee eo n e AQ 7 1 ERRORS DETECTED DURING STARTUP TESTS 40 7 2 ERRORS DETECTED DURING TREATMENT TESTS 41 7 3 ERRORS DETECTED DURING POST TREATMENT TESTS 41 7 4 ERRORS DETECTED DURING PERIODIC TEST WHEN NO TREATMENT IS RUNNING oo 42 Technical specification 43 Stabilizor TT mite sue ci iai heed wara Oe a a a 43 License information ooo ooooo oo 44 Ordering information 46 NOTES scones ei line eni eis 46 Stabilizor T1 DPM 0008 Edition 3 Introduction 1 1 Introduction Biological changes begin from the moment a tissue sample is removed from its native environment and although conventional snap freezing may temporarily pause biological change enzyme activity will return as a sample thaws during preparation for an
3. Remove the cord from the power socket Move the instrument to the new location Insert the cord into a grounded power outlet O dl Km O N Turn the instrument on using the power switch The instrument performs a self test and then stops in running mode ready to start a run Stabilizor T1 DPM 0008 Edition 3 13 denator 4 Software 4 1 GENERAL FUNCTION Stabilizor T1 is controlled via the touch screen CARD NAME CARD NUMBER CARD TYPE TISSUE METHOD FRESH COMP 11 Figure 4 The touch screen of Stabilizor T1 main screen No ose No Descriprion 2 On A Ww 14 Current date and time Buttons with editable text Heating block temperatures 8 Ex vivo button with ex vivo time set upper and lower Selected method 9 Method selection button External USB flash drive connected 10 Start button Error message symbol 11 Options button Ex vivo time To edit card name or card number press the button to display a key pad view To select method press the method selection button to display a new view with selection buttons For detailed information see Section 4 2 1 The ex vivo button display the current ex vivo time Press the button to reset the ex vivo time Press the error message symbol to display the error message To the left there is current information time date heating block temperature and selected method All sample data is saved and can be viewed later see Section 4 2 5 Stabilizor T
4. SIABILIZOR T1 User Manual denalor Content 1 Introduction es 5 2 Safety information 6 2 1 DISCLAIMER 2 2000 6 2 2 IMPORTANT USER INFORMATION 6 2 2 1 Intended use 0 00000 6 2 2 2 Warning caution and note definitions 6 2 2 3 Recycling o 7 2 3 LIST OF WARNINGS AND CAUTIONS 8 3 Stabilizor T1 instrument 9 3 1 MAIN PARTS 9 3 1 1 Functions and descriptions of main parts 10 3 2 INSTRUMENT SITE REQUIREMENTS 12 3 3 INSTALLING THE INSTRUMENT 13 3 4 MOVING THE INSTRUMENT 13 4 Software oooo ooo 14 4 1 GENERAL FUNCTION 14 4 2 RUNNING MODE cee eaee 15 4 2 1 Stabilization SettingS 15 42 2 Exvivotime cee eee eae 20 4 2 3 Start treatment 4 20 4 2 4 Sample treatment 21 4 2 5 Log IES creyeron ses ee ea oe in eee eae 22 AS OPTIONS cosmos mua o eee eee KA at MA 23 4 3 1 Service mode 0 00 0 a 24 4 3 2 Transport mode 0 0 0 eee 25 4 3 3 Software upgrade 26 5 Heat stabilizing tissue samples 27 5 1 PREPARATIONS saggia dae dea heat mee dears 21 5 1 1 Starting the instrument 27 5 1 2 Preparing the Maintainor card 27
5. 107 Card shuttle sensors failed and are therefore not 108 performing correctly Contact service 113 115 116 109 Start button Physical Start button was not recognized during startup The function is disabled until next restart Note that Start is still available on the screen Contact service if error remains after restart Stabilizor T1 DPM 0008 Edition 3 39 denator 7 2 ERRORS DETECTED DURING TREATMENT TESTS All errors in this category will be shown when they are detected and are saved in the log file entry for the treatment that was affected 200 201 202 204 203 205 Contact vacuum The contact vacuum holding the Maintainor card in position at treatment has not been reached hence the sample heating could have been affected A possible cause is a damaged Maintainor card Rerun current sample in a new Maintainor card If problem remains exchange the O ring Contact service if error remains after exchange Cavity vacuum The air evacuation in the cavity failed or was too low A possible cause is a leaking Maintainor card either from damaged top foil or from unproper closing Rerun current sample in a new Maintainor card to avoid undertreatment Contact service if error remains Card Shuttle Card shuttle system did not reach travel positions at the expected time Check for obstacles and rerun sample Contact service if error remains Sample size The sample size is above the specified size
6. Stabilizor T1 Dimensions Net weight Mains power requirements Fuses Battery Power consumption Ambient temperature Maximum relative humidity Touch screen dimensions Vacuum Noise Laser Embedded processor 465x306x 143 mm LxWxH see also Figure 3 6 7 kg 100 240 V 50 60 Hz T4A H 250V CR2032 350 W max 70 W typical use 10 to 40 C 80 non condensing 4 3 2 30 mbar underpressure applied to Maintainor card 45 dB A Class 1 laser 1 GHz x86 1 GB RAM Flash based solid state hard drive Linux base open source software Stabilizor T1 DPM 0008 Edition 3 License information 9 9 License information License texts referenced below are included on built in memory stick and at LICENCE_GPL2 txt http www gnu org licenses old licenses gpl 2 0 html LICENCE _LGPL2 1 txt http www gnu org licenses lgpl 2 1 html LICENCE_GPL3 txt http www gnu org licenses gpl html LICENCE_RXTX txt http users frii com jarvi rxtx license html LICENCE JAVA txt http java sun com javase 6 jdk 6u3 license txt Component IO Controller Firmware The contained firmware of this product uses GPL 2 0 and LGPL 2 1 licenced components and is itself licenced under GPL 2 0 licence Full licence text available on built in memory card and at http www gnu org licenses old licenses gpl 2 0 html Licences Interrupt UART library with receive transmit circular buffers GPL 2 0 http nomepage hispe
7. Contact Denator application specialist for recommendations on large sample treatment Heating System Upper heater did not reach the calculated position Rerun current sample to avoid undertreatment Contact service if error remains 7 3 ERRORS DETECTED DURING POST TREATMENT TESTS 40 All errors in this category will be shown when they are detected and by pressing the error message symbol later 300 Needle Needle obstructed Inspect needle for obstructions and damage See Section 6 3 for instructions on how to exchange the needle Restart instrument after replacement and make a blank run with a new Maintainor card Contact service if error remains after exchange and startup Stabilizor T1 DPM 0008 Edition 3 Diagnostics 7 7 4 ERRORS DETECTED DURING PERIODIC TEST WHEN NO TREATMENT IS RUNNING All errors in this category can also be displayed from the status panel by pressing the error message symbol 400 Heating system 401 One of the heating system sensors has reported values out 402 of specification and is not performing correctly Restart instrument to reset sensors Contact service if error remains 403 Instrument temperature Temperature inside instrument is too high Shut off the instrument to cool down Check that the ambient temperature is within the specification Restart the instrument Contact service if error remains Stabilizor T1 DPM 0008 Edition 3 41 denator 8 Technical specification 42
8. OPTIONS and then SERVICE MODE see Section 4 3 1 3 Turn off the instrument 34 Stabilizor T1 DPM 0008 Edition 3 Maintenance 6 4 Push the sample tray gently forward di Caution It is only possible to move the sample tray if the instrument was set to Service mode before it was turned off 5 Remove the tube from the tube interlock and the vacuum connection 6 Carefully remove the needle device in the tube direction 7 Dispose of the needle device in accordance with local regulations 8 Fit the top of the new needle into the hole of the needle guide and gently insert the needle device into the needle holder gt Make sure that the needle is inside the hole of the needle guide Stabilizor T1 DPM 0008 Edition 3 35 denator 9 Holddownthe needle device while snapping the vacuum tube into the tube interlock 10 Make sure that the needle device is flush with the needle holder Y X gt Correctly fitted needle device to the left Otherwise re fit the tube into the tube interlock so a downward pressure is applied to the needle device 11 Fit the tube into the vacuum connection A x 36 Stabilizor T1 DPM 0008 Edition 3 Maintenance 6 6 4 REPLACE THE BATTERY 1 Turn the instrument off 2 Remove the rubbergasket which gives access to the battery on the internal PC 3 Exchange the battery with a similar CR2032 4 Attach the rubber gasket again 5 Turn the instrument on and adju
9. Stabilizor T1 DPM 0008 Edition 3 31 denator 2 In next view press SERVICE MODE The upper heating block will be lifted to an extra high level 3 Turn off the instrument using the Power switch as instructed on the screen and disconnect the mains cord 4 Allow the heating block to cool for 30 minutes 5 Remove the two screws on the back of the instrument using a Torx T20 tool 6 Lift off the top cover Warning Hazardous voltage Stabilizor T1 contains mains voltage of up to 240 V ac Disconnect the power cord before removing the top cover Warning Laser inside The instrument must always be turned off when the top cover is removed Warning Hot parts inside Do not remove the top cover unless the instrument has been turned off and allowed to cool down for 30 minutes gt bb 32 Stabilizor T1 DPM 0008 Edition 3 Maintenance 6 7 Remove the white O ring 9 Putona new O ring Make sure that the O ring is not twisted and that it is fitted securely in the groove Stabilizor T1 DPM 0008 Edition 3 33 denator 6 3 CHANGING THE NEEDLE DEVICE Warning Sharp needle Take care when handling the needle device to avoid injury Figure 8 Needle overview and Instrument part kas Instrument part Needle guide Tube interlock 2 Needle holder 5 Vacuum tube 3 Needle device 6 Vacuum connection 1 Make sure that there is NOT a Maintainor card in the sample tray 2 Set the instrument to Service mode by pressing
10. card 5 Close the Maintainor card by pressing the lid down Ensure that the lid has sealed completely by gently pressing down the cover lid encircling the sample chamber using one or two fingers Caution Do not press the inner edge of the cavity The upper plastic can be damaged leading to leakage of air during the heating process 5 2 2 Sample stabilization Immediately start the heat stabilization process 6 If not already done place the Maintainor card in the sample tray 28 Stabilizor T1 DPM 0008 Edition 3 Heat stabilizing tissue samples 5 7 Start the run by pressing the start button above the screen or by pressing START on the screen 8 During stabilization air is evacuated from the card the sample is moved automatically into the heating compartment and treated according to set parameters 9 When the sample tray returns to the starting position and the illumination turns green remove the Maintainor card from the tray 10 Check that there is vacuum in the sample compartment no vacuum indicates need of needle device change or remounting of the needle device 11 When the heat stabilization process is done store the Maintainor card with the heat stabilized sample at 80 C for long term storage or prepare sample for analysis Caution Do not put the Maintainor card into liquid nitrogen The Maintainor card can only withstand temperatures down to 80 C 12 The next sample if prepared can be heat stabil
11. of the sample and adjustment of the stabilization time for inactivation To be used for all tissue below 20 C down to 80 C that should maintain its structure i e that must be minimally compressed The method includes automatic measurement of the sample and adjustment of the stabilization time for inactivation Manual mode where all parameters can be set by the user To be used for setting a sample under vacuum without heating Can be used before re freezing previous stabilized samples 17 denalor 18 1 2 3 Press the method text to open the method selection dialog FRESH COMP Select method and return to the main screen ARIA AS na 2010 02 03 A E i 08 55 05 QUICK FRESH COMPRESS SET Shay E UPF 95 0 C ps Sa QUICK FROZEN COMPRESS LO SS O C FRESH COMP FRESH STRUCTURE PRESERVE FROZEN STRUCTURE PRESERVE A CUSTOM CANCEL DOS If CUSTOM is selected continue with setting the parameters on the new screen before pressing OK to return to the main screen In custom all parameters are set individually and no algorithms is present 2011 10 25 1a HEATER TEMP 95 C LI gt SET 95 C UP 95 0 C PEATA AC 90 NEN LO 95 0 C UPPER HEATER ON Seale YACUUM PACK MES CANCEL FRESH COMP A 00 46 Stabilizor T1 DPM 0008 Edition 3 Software 4 4 2 2 Ex vivo time In this context the ex vivo time is the time from the sacrifice of the experimental animal o
12. release date of the product or binaries in question 44 Stabilizor T1 DPM 0008 Edition 3 Ordering information 10 10 Ordering information Product ordermumber Stabilizor T1 Instrument Maintainor Tissue card Stabilizor T1 Maintenance Kit Stabilizor Peptide Extraction Kit Tissue Stabilizor 2D GE Extraction Kit Tissue NOTES Stabilizor T1 DPM 0008 Edition 3 DST0001 DMT0001 DST0016 DKT0001 DKT0002 45 denator 46 Stabilizor T1 DPM 0008 Edition 3 denalor Stabilizor T1 User Manual DPM 0008 Edition 3 February 2013 Denator AB First published September 2008 Stabilizor and Maintainor are trademarks of Denator AB All third party trademarks are the property of their respective owners Denator AB Contact Arvid Wallgrens Backe 20 SE 413 46 Gothenburg Sweden Phone 46 0 31 412 841 Fax 46 0 31 412 840 E mail inform denator com order denator com Website www denator com Support E mail support denator com application denator com
13. 1 DPM 0008 Edition 3 Software 4 Maintenance requests and failure messages are automatically displayed on screen when needed See additional information Section 4 3 and Section 6 4 2 RUNNING MODE In running mode the instrument is ready for stabilizing samples To set the instrument in Running mode If the instrument is not turned on 1 Turn on the instrument see Section 3 3 The instrument automatically enters Running mode displaying the main screen CARD NAME NO NAME CARD MUMBER 0000 CARD TYPE TISSUE METHOD FRESH COMP 2 Wait until the startup sequence has finished and the heating blocks have reached the correct temperature normally 95 C Stabilization cannot be performed until the set temperature of the heating blocks has been reached as indicated by the text WAIT on the start button 4 2 1 Stabilization settings There are three settings to be entered for each sample before starting a stabilization e Sample name e Card number e Method Data is entered on the screen via the key pad available in the software To be able to enter data the Stabilizor T1 must be in running mode The information is saved in the log file see Section 4 2 5 Stabilizor T1 DPM 0008 Edition 3 15 denator 1 Press the sample name text to open the keypad 2 Enter the sample name If required press the 123 selection button to be able to enter digits and symbols Press OK to save the name and return to the
14. 2012 06 17 xls is saved into a folder called Denator created by the software All runs during a day are saved into the same log file The log file includes the following data for each sample Time Sample name Maintainor ID s Ex vivo time Run time SET Temperature Actual Temperature Vacuum Sample height Method used and Comments The log files are saved as Microsoft Excel spreadsheet xls Stabilizor T1 DPM 0008 Edition 3 21 denator 4 3 OPTIONS CARD MAME NO NAME CARD NUMBER 0000 CARD TYPE TISSUE METHOD FRESH COMP Figure 5 Press Options to get to the Options screen Figure 6 The Options screen SERVICE MODE TRANSPORT MODE HW SW REVISION SCREEN CALIBRATION HEIGHT CALIBRATION ADJUST CLOCK EXIT 22 Function description Use to clean the instrument see Section 4 3 1 and or change needle device see Section 6 3 or O ring see Section 6 2 Use to move the instrument See Section 4 3 2 Use to check software version and to update the software See Section 4 3 3 Use to align the screen Displays height calibration values Use to adjust the time and date Use to exit the Options screen Stabilizor T1 DPM 0008 Edition 3 Software 4 4 3 1 Service mode In service mode the instrument is prepared for maintenance e g change of the O ring or replacing the needle see Section 6 The upper heating block is raised to an extra high level and the sample tray is releas
15. alysis Denator has developed an additive free heat stabilization technology that preserves the quality of biological tissue samples from moment of excision throughout the entire workflow Denator s heat stabilization technology utilizes rapid conductive heating to generate a fast homogenous thermal denaturation of proteins This results in complete permanent denaturation and thereby inactivation of all enzymes such as proteases peptidases phosphatases that could cause further biological changes to the tissue sample ex vivo Since the system is additive free meaning does not require any addition of chemical inhibitors potential interference with downstream analysis is removed Biomarkers peptides and posttranslational modifications such as phosphorylated and acetylated proteins are preserved close to their in vivo state throughout the entire workflow After heat stabilization of either fresh or frozen tissue samples in the Stabilizor system biological components can be extracted and analyzed using common buffers and techniques Compatibility with a number of downstream analytical techniques has been verified including mass spectrometry e g phospho shot gun MALDI Imaging etc Western Blot with phospho specific antibodies 1D and 2D gels with phospho specific stains and reversed phased protein arrays RPPA For information on how the Stabilizor system can be utilized in different applications please visit Denator s website www d
16. ed The instrument will not move any parts when in service mode However it is recommended to turn the instrument off after setting it into service mode Service mode should also be used when cleaning is needed or if something is dropped into the instrument To set the instrument in Service mode 1 From the main screen press the OPTIONS button CARO NAME MOTOS CARO NUMBER 0000 CARD TYPE TISSUE METHOD FRESH COMP 2 Press the SERVICE MODE button and follow the instructions on the screen Stabilizor T1 DPM 0008 Edition 3 23 denator 4 3 2 Transport mode When the instrument needs to be moved Transport mode should be used It is recommended that transport mode is used during longer storage periods to avoid damage to the sample tray and needle To set the instrument in Transport mode 1 Place a closed empty Maintainor Tissue card on the sample tray 2 From the main screen press the OPTIONS button CARO NAME DO Noname CARD NUMBER 0000 CARD TYPE TISSUE METHAL FRESH COMP 3 Press the TRANSPORT MODE button 4 Follow the instructions on the screen 5 Move the instrument according to Section 3 4 Storage Stabilizor T1 should be stored in 10 to 40 C and max 80 relative humidity 24 Stabilizor T1 DPM 0008 Edition 3 Software 4 4 3 3 Software upgrade Software upgrades will be distributed by Denator Caution Do NOT turn off the instrument during the upgrade as this may cause irrever
17. ed ch peterfleury uartlibrary zip LICENCE_GPL2 txt 12C interface using AVR Two Wire Interface TWI hardware GPL 2 0 http www procyonengineering com embedded avr avrlib avrlib zip LICENCE_GPL2 txt Component OS The OS of this product uses GPL licensed software Licences OS Puppylinux GPL 2 0 GPL 3 0 BSD http puppylinux org LICENCE_GPL2 txt LICENCE GPL3 txt Component GUI Some linked components of GUI uses LGPL software but is closed source itself Licences RXTX LGPL 2 1 amp Extensions http rxtx qbang org LICENCE_RXTX txt JAVA Java LICENCE_JAVA txt Java Excel API LGPL 2 1 http jexcelapi sourceforge net LICENCE LGPL2_1 txt WRITTEN OFFER For binaries received from Denator on physical device or physical media that are licensed under any version of the GNU General Public License GPL or the GNU LGPL you can receive a complete machine readable copy of the source code by sending a written request to Stabilizor T1 DPM 0008 Edition 3 43 denator Denator Biotech Center Arvid Wallgrens Backe 20 SE 413 46 Gothenburg Sweden Your request should include i the name and version number of the product containing the covered binary ii your name iii your company if applicale and iv your return mailing and email addres if available We may charge you a nominal fee to cover the cost of the media distribution Your request must be sent withing 3 years of the
18. enator com or contact us at application denator com Stabilizor T1 DPM 0008 Edition 3 5 denator 2 Safety information 2 1 DISCLAIMER Denator will use reasonable efforts to include accurate and up to date information in this document Denator makes no warranties or representations of any kind as to the accuracy completeness or usefulness of the information The information shall not be considered to constitute a warranty or representation of any kind legal or other or a quality specification Neither Denator nor any party involved in creating producing or delivering this document shall be liable for any damages arising out of use of this document or any errors or omissions in the content thereof Stabilizor and Maintainor are trademarks of Denator AB All other trademarks are the property of their respective owners Products and technologies from Denator are patent pending All infringements are prohibited and may be prosecuted Please contact Denator AB for further details Products are for research use only Denator AB 2013 2 2 IMPORTANT USER INFORMATION 2 2 1 2 2 2 A O Intended use Stabilizor T1 and all associated products from Denator AB are for research use only Do not use in diagnostic procedures for clinical purposes Warning caution and note definitions Warning A warning indicates that there is a risk of personal injury if the instructions are not followed It is very important to address all
19. he stabilization time to optimize sample stabilization Used for connection of external accessories 11 denator 3 2 INSTRUMENT SITE REQUIREMENTS 143mm Figure 3 Dimensions of Stabilizor T1 For proper operation Stabilizor T1 requires an ambient temperature of 10 to 40 C and 80 maximum relative humidity Make sure there is adequate air circulation around the instrument For indoor use only N Warning Do not block the rear panel of the instrument The power switch must always be easily accessible Power requirements 12 Mains voltage 100 240 VAc auto range 50 60 Hz Connect only to grounded power outlets Power consumption 350 W max 70 W typical use Use a slow 20x5 mm ceramic fuse T4A H that holds for 250V Stabilizor T1 DPM 0008 Edition 3 Stabilizor T1 instrument 3 3 3 INSTALLING THE INSTRUMENT 1 Unpack the instrument Place the instrument on an appropriate surface according to Section 3 2 Insert the power cord into the instrument and into a grounded power outlet Turn the instrument on using the power switch The instrument will perform a self test Wait until it is done O Aa A O N The instrument is ready for use when the initial screen is lit 3 4 MOVING THE INSTRUMENT 1 Caution The instrument can be damaged if the instructions for moving the instrument is not followed 1 Set the instrument to transport mode see Section 4 3 2 Turn the instrument off using the power switch
20. ized as soon as the processed Maintainor card has been removed 5 3 LOG FILES Log files are automatically saved on the USB flash drive see Section 4 2 5 5 4 EXTRACTION OF PROTEINS FROM HEAT STABILIZED TISSUE SAMPLES To ensure the most efficient extraction of proteins from heat stabilized tissue samples the following must be observed 1 Use a denaturing buffer gt 8M Urea gt 1 SDS gt 6M GuHCI Buffers based on gt 8M urea gt 6M GuHCI or gt 1 SDS have proven to be effective When using SDS buffers it is recommended to maximize their effectiveness by Stabilizor T1 DPM 0008 Edition 3 29 denator 30 adding heated buffer gt 90 C to the sample preferably followed by a second homogenization step using ultrasonication Other components such as detergents and buffering agents can be added as long as the concentrations of the denaturing agents are not affected 2 Use a buffer to sample ratio greater than 10 510 ul buffer mg sample For maximum extraction efficiency it is important to add at least 10 times the amount of sample i e 10 ul of buffer for each mg of sample 3 Ensure thorough homogenization using ultrasonication grinding or ball mill Although heat stabilized samples will usually homogenize easily it is very important to ensure that the initial homogenization step is extremely thorough to facilitate the resolubilization of proteins Softer tissue types such as brain can be homogenized using a microtip
21. main screen or press NUMBER to save name and go directly to entering the card number ss E ES O 0 OG NUN ES All Maintainor cards have room for customer IDs and or bar codes 1 Press the card number text to open the key pad 0000 16 Stabilizor T1 DPM 0008 Edition 3 Software 4 2 Enter the card number and press OK to save the number and return to the main screen or press NAME to save number and go directly to enter card name Once a sample is stabilized the card number is automatically increased with 1 to simplify the settings for next sample Set method There are five different stabilization methods and one method for vacuum treatment Method Recommended use and description QUICK FRESH To be used for fresh tissue that does not need to keep its COMPRESS QUICK FROZEN COMPRESS FRESH STRUCTURE PRESERVE FROZEN STRUCTURE PRESERVE CUSTOM CUSTOM gt VACUUM PACK Stabilizor T1 DPM 0008 Edition 3 structure The method includes automatic measurement of the sample and adjustment of the stabilization time for inactivation To be used for all tissue below 20 C down to 80 C that does not need to keep its structure The method includes automatic measurement of the sample and adjustment of the stabilization time for inactivation To be used for fresh tissue that should maintain its structure i e that must be minimally compressed The method includes automatic measurement
22. ontains mains voltage of up to 240 VAC Disconnect power cord before removing the top cover Warning Laser inside The instrument must always be turned off before the top cover is removed Warning Sharp needle To avoid injury take care when handling the needle device Warning Ensure that all instrument site requirements are fulfilled before starting the instrument Caution Handle the needle device carefully to avoid bending the needle Caution The instrument can be damaged if the instructions for moving the instrument are not followed Caution Do not put the Maintainor card into liquid nitrogen The Maintainor card withstands temperatures down to 80 C Stabilizor T1 DPM 0008 Edition 3 Stabilizor T1 instrument 3 3 Stabilizor T1 instrument 3 1 MAIN PARTS 12 6 3 Figure 1 Main parts of Stabilizor T1 No instrument port CN CT 1 Touch screen Illumination 2 Start buttons 6 Ex vivo button 3 Sample tray 7 Top cover 4 USB ports 8 Needle device Stabilizor T1 DPM 0008 Edition 3 denator 12 13 10 9 Figure 2 Main parts of Stabilizor T1 Co Instrument part band Instrument part Power switch Ethernet connection 10 Power connection 13 Accessory connection 11 Top cover screws 3 1 1 Functions and descriptions of main parts Part Function description Touch screen Displays the control software and command buttons see Section 4 for detailed information Ex vivo button Resets Restart
23. r the collection of a tissue sample until the sample is stabilized The ex vivo time is reset to zero by pressing the ex vivo button on the screen SA DO noname CARD NUMBER 0000 CARD TYPE TISSUE METHOD FRESH COMP It is important to start the ex vivo time measurement just prior to the sacrifice of the animal or removal of the sample whatever occurs first 4 2 3 Start treatment To start a sample run both the START button on the screen and the physical Start button can be used Stabilizor T1 DPM 0008 Edition 3 19 denator 4 2 4 Sample treatment During sample treatment the progress is shown on the screen PROCESSING SAMPLE TIME LEFT EX WO TIME HEATER TEMP HEATER POSITION Se vee IEI fa SAHRO MEKA Press CARD INFO to update card name and card number for the sample in process during sample treatment 20 Stabilizor T1 DPM 0008 Edition 3 Software 4 4 2 5 Log files A log file including information on the samples are automatically saved to an external USB flash drive if inserted If no external USB flash drive is inserted into the instrument the log file is saved on the internal USB flash drive until one is inserted A large number of stabilization runs can be saved on the internal USB flash drive To minimize the risk of accidental loss of log data due to any unexpected failure always have an external USB flash drive inserted into the instrument The log file marked with the date e g
24. s the ex vivo timer Start button Starts the stabilization process Sample tray Holds the Maintainor card and moves the Maintainor card into and out of the heating block compartment Maintainor card Container for the sample during the heating process and after Needle parts e Needle device Penetrates the vacuum seal to remove air from the Maintainor card Needle holder and Needle guide Holds the needle device in the correct position e Needle shuttle lever Moves the needle device into and out from the vacuum channel of the Maintainor card 10 Stabilizor T1 DPM 0008 Edition 3 Part Heating compartment O ring Illumination USB port Laser Accessory connection Stabilizor T1 DPM 0008 Edition 3 Stabilizor T1 instrument 3 Function description Chamber for the heating blocks with a controlled temperature environment Contact vacuum seal placed on the lower heating block for optimal contact between the Maintainor card and the lower heating block Indicates treatment process status e Blue The instrument is ready and a Maintainor card can be placed in or removed from the sample tray e Green The sample cycle is processed e Red The instrument is initializing or busy 3 ports available Used for USB flash drive to collect and store the treatment log file Software updates are installed via a USB flash drive Measure the thickness of the sample to ensure reproducibility by adjusting t
25. safety issues stated in Warnings Caution A caution indicates that there is a risk of damaging the instrument if the instructions not are followed It is important to address all issues stated in Cautions in order to keep your equipment in good condition gt A note indicates important information for improving your results or facilitating your work Stabilizor T1 DPM 0008 Edition 3 Safety information 2 2 2 3 Recycling All electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information EE concerning the decommissioning of equipment Stabilizor T1 DPM 0008 Edition 3 T denator 2 3 LIST OF WARNINGS AND CAUTIONS A oo0oobbbbbbb Warning This is a Class A product In a domestic environment it might cause radio interference in which case the user might be required to take appropriate measures Warning No user serviceable parts inside All repairs should be done by personnel authorized by Denator Do not open any covers or replace parts unless specifically stated in the instructions Warning Do not block the rear panel of the instrument The power switch must always be easily accessible Warning Hot parts inside Do not remove the top cover unless the instrument has been turned off and allowed to cool down for 30 min Warning Hazardous voltage Stabilizor T1 c
26. sible malfunction 1 Remove any inserted USB flash drives before starting the upgrade 2 Copy the program file DST0001_SPxxxx jar to root level of a USB flash drive Make sure the file is named DST0001_SPxxxx jar No un zipping of the file is needed and no zip extension should be added 3 Insert the USB flash drive with the upgraded software DN Wait until the USB symbol appears on the screen approximately 10 seconds Press the OPTIONS button Press the HW SW REVISION button Document the current version of software Press the UPDATE button Let the installation run Do NOT turn off the instrument during the update O 0 N O QO The installation is successfully completed when the text Restart the instrument update complete appears on the screen 10 Restart the instrument using the power switch Stabilizor T1 DPM 0008 Edition 3 25 denator 9 Heat stabilizing tissue samples 5 1 PREPARATIONS 5 1 1 Starting the instrument Warning Ensure that all instrument site requirements including a grounded power outlet are fulfilled before starting the instrument see Section 3 2 1 Insert a USB flash drive for the log file in a USB port 2 Turn the instrument on using the power switch 3 The system check is automatically performed During the system check the illumination is first red while the sample tray is moved in and out and then changes to blue The heating blocks are heated to the set temperature
27. sonication rod whereas a micropestle grinding or ball mill should be used for firmer tissue types such as liver or heart Specific protocols for heat stabilization homogenization and extraction prior to LCMS or 2D GE analysis are supplied with the Stabilizor Peptide Extraction Kit and Stabilizor 2D GE Extraction Kit respectively Additional recommendations for other analytical downstream analysis can be retrieved at www denator com or via application denator com Stabilizor T1 DPM 0008 Edition 3 Maintenance 6 6 Maintenance 6 1 IMAINTENANCE AND SERVICE Service of Stabilizor T1 should be performed by service engineers from Denator Contact information to Denator is provided on the back cover The following maintenance can be performed by the customer e Changing the O ring including cleaning heat blocks see Section 6 2 e Changing the needle device see Section 6 3 e Cleaning the instrument see Section 6 5 The Stabilizor T1 should always be put into service mode see Section 4 3 1 before performing any maintenance or cleaning 6 2 CHANGING THE O RING For optimal contact and thereby heat transfer between the lower heating block and the Maintainor card during stabilization the space between the two parts sealed by an O ring is evacuated during the stabilization 1 With the Stabilizor T1 instrument turned on press OPTIONS in the main screen DOR RIESE MTS CARD NUMBER 0000 CARD TYPE TISSUE METHIL FRESH COMP
28. st the date and time under OPTIONS Stabilizor T1 DPM 0008 Edition 3 37 denator 6 5 CLEANING THE INSTRUMENT If the instrument or area under the sample tray needs to be cleaned set the instrument in service mode see also Section 4 3 1 With the instrument on 1 With the instrument on press OPTIONS in the main screen SA MET CARD NUMBER 0000 CARD TYPE TISSUE METHOD FRESH COMP 2 In next view press SERVICE MODE 3 Wipe the area under the sample tray with a tissue moistened with 70 EtOH 4 If required clean the outside of the instrument as well 5 Follow the instructions on screen to return to running mode With the instrument turned off 1 Move the sample tray out of the way manually gt It is only possible to move the sample tray if the instrument was set to Service mode before it was turned off 2 Clean the area as in step 3 and 4 above 38 Stabilizor T1 DPM 0008 Edition 3 Diagnostics 7 7 Diagnostics 7 1 ERRORS DETECTED DURING STARTUP TESTS All errors in this category will be shown after the self test is completed and can also be displayed from the status panel by pressing the error message symbol later 100 Contact Vacuum Air flow from the lower heatblock is obstructed Restart the instrument Contact service if error remains Backup battery Battery power is too low to keep the clock settings between startups Replace battery see Section 6 4 for instructions 106 Card shuttle
Download Pdf Manuals
Related Search