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1. 1 Connect the six contact connector into the sensor Connect the 9 volt connector to a 9 volt battery As an alternative it can be connected to a regulated power supply 3 Connect the db 9 connector to the host PC Use a USB to RS232 adapter cable if necessary 4 Use the probes on a digital multimeter to see analog output from the sensor The inside of the RCA is power and the outside is ground Section 4 General information 4 1 Warranty Revised editions of this user manual are on the manufacturer s website This sensor is warranted against defects in materials and workmanship for one year from the date of purchase The warranty is void if the manufacturer finds the sensor was abused or neglected beyond the normal wear and tear of deployment 4 2 Service and repair The manufacturer recommends that sensors be sent back to the factory annually to be cleaned calibrated and for standard maintenance Do the steps below to send a sensor back to the manufacturer 1 Contact the manufacturer for a Return Merchandise Authorization RMA Note The manufacturer is not responsible for damage to the sensor during return shipment 2 Remove all anti fouling treatment from the sensor before sending it back to the manufacturer Note The manufacturer will not accept sensors that have been treated with anti fouling compounds for service or repair This includes tri butyl tin marine anti fouling paint ablative coatings etc 3
2. Use the sensor s original ruggedized shipping case to send it back to the manufacturer 4 Write the RMA number on the outside of the shipping case and on the packing list 5 Use 3rd day air to ship the sensor back to the manufacturer Do not use ground shipping 6 The manufacturer will supply all replacement parts and labor and pay to send the sensor back to the user via 3rd day air shipping 4 3 Waste electrical and electronic equipment Electrical equipment that is marked with this symbol may not be disposed of in European public disposal systems In conformity with EU Directive 2002 96 EC European electrical equipment users must return old or end of life equipment to the manufacturer for disposal at no charge to the user To recycle please contact the manufacturer for instructions on how to return end of life equipment manufacturer supplied electrical accessories and auxiliary items for proper disposal General information 10 WET Labs Inc P O Box 518 O 5 Philomath OR 97370 WWW Tel 541 929 5650 Fax 541 929 5277 www wetlabs com WET Labs Inc 2013 All rights reserved Printed in U S A
3. can damage the eyes Keep products that have UV LEDs away from children pets and other living organisms Wear polycarbonate UV resistant safety glasses to protect the eyes when a UV LED is on NOTICE Do not supply more than 15 VDC to the sensor More than 15 VDC will damage the sensor 2 1 Analog functional check Supply 12 VDC to the sensor The sensor comes on Use the probes on a digital multimeter DMM to touch the RCA connector on the auxiliary leg s of the test cable Refer to the section on the Test cable on page 8 for details about test cables Put the red signal probe in the RCA connector and the black ground on the outside The DMM shows near 0 VDC Put the fluorescent stick for fluorometers or a solid object near the light source of the sensor The DMM shows near 5 VDC Le gt 2 1 1 Digital output check 1 Connect the test cable to the sensor Refer to the section about the Test cable on page 8 for information on the test cable 2 Connect the DB 9 connector on the test cable to a PC 3 Connect the test cable to a regulated power supply or a 9 V battery 4 Start a terminal communication program such as HyperTerminal or Terra Term Select a Bits per second 9600 b Data bits 8 c Parity none d Stop bits 1 e Flow control none 5 Turn the power supply on if necessary The digital output is a column of zeros 6 Put the fluorescent stick in the flow tube of the se
4. the concentration of the sample of interest output clean water offset x scale factor concentration of solution For example ifthe clean water offset is 60 counts and known concentration of 65 ppb gives an output of 3500 counts 65 ppb 3500 60 counts 0 018 scale factor 7 Record the scale factor and offset to apply to the data collected by the sensor 3 2 1 1 Flow rate dependence Fluorescent signals from phytoplankton have some dependence on the flow rate of the sample water through the flow tube The manufacturer recommends the user use a small pump that has a known flow rate The figure below shows that the output voltage of the sensor changes approximately 15 18 as flow rate changes from approximately 3 to 30 ml sec This data is based on samples with Thalassiosira weissflogii Reference topics Flow Rate Dependence Normalized Output Voltages 1 20 1 15 1 10 1 05 1 00 0 95 0 90 0 00 5 00 10 00 15 00 20 00 25 00 30 00 Flow Rate ml sec It is possible to use the sensor in a profiling mode without a pump However it is difficult to keep a constant flow rate through a sensor cage because of sea states and ship motion Trendline is derived from data collected from three fluorometers Relative Response 3 3 Test cable Use a test cable to set up and test the sensor before deployment 1 six contact connector 3 db 9 serial port connector 2 9 volt battery connector 4 RCA connector
5. WET Labs WETStar User manual 12 2013 Edition 1 Table of Contents Section T Specifi atO S Ea 3 a Fes 11661121111 SERRES En e nee RT HER ER ste EUR site As te os e re ate ad e ste 3 1 11 Bulkhead connectorn an 1 1 1 1 astra 3 AND 216001691 ARLES A A TE E dans te 2 3 1 83 COMMUNICA ION Sicilia E A 3 1 4 000 3 Section 2 Operation ee 5 2 1 Analog f nelional check cnica ae re engen 5 vari ead O A en a re alas 5 2 2 Deployment ee ee ee 5 2321 Data collection o hen Ba OR Bee ie 6 2 3 Clean the sensor unten ada 6 2 3 1 Bulkhead connector maintenance sisi 6 Section 3 Reference TOPICS 7 31 Delivered eS coi 00 tir e 7 3 2 Charact riZati n 4 3808 ER FEPEERELRLRRTRLEETELELERFLTELERFELERFFRLEFFERELFERERTLFELHLRERFERFTTLFETNERERUTRRERFFETLEFFRERERER 7 3 2 1 Field ch ract rization essen nennen as ng anna free 7 3 2 1 1 Flow rate dependence ses 7 33T TOSUCADIG a a 8 Section 4 General information 00 ccccccccecc cece cece eeeneeceeeeeeeeeseeeeeeeeeceeeecaesecaeeecaeeeseeesneeeeneeesaes 9 BSL LEE D S LS LS x 9 4 2 Service and E sr rein an er rt ds 9 4 3 Waste electrical and electronic equip
6. late the sensor output with depth or time 2 3 Clean the sensor Clean the quartz flow tube carefully It scratches and breaks easily 1 After each cast or exposure to natural water flush the sensor with clean fresh water 2 Clean any grease or oil with soapy water a Solvents such as methanol can be used to clean the flow tube Use a long cotton swab to reach the length of the flow tube b Rinse the sensor thoroughly inside and outside 3 Letthe sensor dry in air 2 3 1 Bulkhead connector maintenance Lubricate the mating surfaces of bulkhead connectors at regular intervals with pure silicone spray only Allow the contacts to dry before they are connected Make sure that the pins have no corrosion which looks green and dull Make sure that the rubber seals on the pins are not delaminated Connectors should mate smoothly and not feel gritty or too resistant The manufacturer recommends 3M Silicone Lubricant spray UPC 021200 85822 Other silicone sprays may contain hydrocarbon solvents that damage rubber DO NOT use silicone grease DO NOT use WD 40 The wrong lubricant will cause the bulkhead connector to fail prematurely and the sensor will flood Section 3 Reference topics 3 1 Delivered items The sensor e dummy plug with lock collar 1 nozzle 6 9 cm 1 nozzle 3 8 cm e fluorescent stick e user manual e characterization page 3 2 Characterization The manufacturer characterizes all fluo
7. ment 9 Table of Contents Section 1 Specifications 1 1 Mechanical Diameter 6 98 cm Length 17 1 cm 25 7 cm with tubing Depth rating 600 m Temperature range 0 30 C Weight in air water 0 8 kg 0 1 kg 1 1 1 Bulkhead connector Pin Function MCBH 6 MP connector 1 Ground Reserved Reserved Voltage in TX Om 4 amp OJN Analog out 1 2 Electrical Input 7 15 VDC Current draw Linearity lt 40 mA standard lt 80 mA digital 99 1 3 Communications Maximum output digital 4095 counts Maximum output analog 5V Response time digital 0 125 seconds Response time analog 0 17 seconds 1 4 Optical Parameter Wavelength EX EM Range Sensitivity Chlorophyll Chl 460 695 nm 0 03 75 0 03 ug L standard 0 06 150 0 03 ug L Colored Dissolved Organic 370 460 nm 0 250 0 1 ppb near coastal Matter CDOM 0 100 0 1 ppb open ocean Uranine UR 485 532 nm 04000 1 ug L Specifications Section 2 Operation This sensor measures the quantity of fluorescence emitted from a given sample of water The sample flows through the sensor and is excited by the sensor s light source This causes the sample to emit absorbed energy as fluorescence CDOM sensors use UV LED light Do not look directly at a UV LED when it is on It
8. nsor The output increases to the maximum output in counts specified for the sensor 2 2 Deployment Deploy the sensor with or without a pump The manufacturer recommends using a pump because it supplies a consistent flow of water through the sensor The manufacturer supplies threaded tubing nipples for the flow tube ports to use with a pump or water trap Note Use an RS232 cable less than 5 m long unless a longer cable has been tested a Operation The manufacturer recommends a flow rate of 25 ml sec using a pump made by Sea Bird Electronics The SBE 05T has an adjustable motor speed so the user can control the flow rate into the sensor Refer to the section on flow rate dependence for more information Ifthe user deploys the sensor in a free flow mode without a pump make sure that the sensor has a clear water path during descent Use a water trap if necessary this is a funnel type device attached to the sensor with the wide end pointed toward the direction of deployment The manufacturer recommends a descent rate of 0 2 1 0 m sec 2 2 1 Data collection Connect digital sensors to a PC or data logger that can receive an RS232 signal at 19200 baud Connection to a PC or data logger also lets the user save the sensor s data Connect analog sensors to a host such as a data logger radiometer or CTD conductivity temperature depth that can digitize the analog output of the sensor A data logger will merge CTD and sensor data and corre
9. rescence sensors using a fluorescing material to make sure that the data that is collected meets the sensor s specifications This information is on the sensor specific characterization page that ships with the sensor 3 2 1 Field characterization The manufacturer recommends that the user perform a field characterization on fluorometers to make sure that the data output is as accurate as possible for the user s application The scale factor and dark counts values can vary depending on the natural water temperature cable length power supply and other factors Do the steps below to field characterize the sensor e x the solution of a known concentration in volts or counts output the measured sample of interest in volts or counts clean water offset the measured signal output in volts or counts of the sensor with clean water in the flow tube scale factor the multiplier in ug L volt ppb L volt OR ug L count ppb L count 1 Connect the sensor to a 16 bit analog to digital converter ADC Look at the output of the sensor in counts in HyperTerminal or other terminal emulator software Get a solution of known concentration x 3 Measure and record this solution using the sensor This value is the output in volts or counts 4 Measure and record the sensor s clean water offset 5 Use this equation to determine the sensor s scale factor Scale factor x output dark counts 6 Use the scale factor to determine
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