Home

BioReactor Simulator - User Manual

Contents

1. TIMER TO CONTROL INTERVALS OF MOTOR REVERSAL The rotary Timer switch can be used to set the time that should elapse before the direction is changed 10 positions are available ranging between 5 sto 1 h Position a ee ee 16 bloprccess ROL FUSE To protect the Motor Controller the Printed Circuit Board PCB 1s fitted with a slow blow 2 A fuse 522 520 If none of the mixers are working even though the power supply is connected this fuse should be replaced The fuse is located inside the Motor Controller which can be opened by removing the four screws placed in each corner of the top cover See picture below Fuse OSTEGIA Vv Vv Ge Q f J k aa Q D MOTOR UNIT Each motor unit has its own driver board which controls the power distribution to the actual motor The switches are explained below ON OFF Direction switch switch Green ON OFF LED 17 DIOPr2cess CONTROL MOTOR UNIT ON OFF SwITCH The ON OFF switch is located on the side of the motor and marked on the very top of the motor unit just above where the actual switch is located When a mixer 1s active and power is supplied an LED next to the ON OFF switch will be green If not all mixers will be used setting the ON OFF switch to OFF can turn each motor off individually The motor unit will make a sound to indicate when this is done It is recommended to set the switch on each Motor Unit to the OFF position before connect
2. Downloadify Client Side File Creation JavaScript Flash Library Copyright c 2009 Douglas C Neiner Permission is hereby granted free of charge to any person obtaining a copy of this software and associated documentation files the Software to deal in the Software without restriction including without limitation the rights to use copy modify merge publish distribute sublicense and or sell copies of the Software and to permit persons to whom the Software 1s furnished to do so subject to the following conditions The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software THE SOFTWARE IS PROVIDED AS IS WITHOUT WARRANTY OF ANY KIND EXPRESS OR IMPLIED INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM DAMAGES OR OTHER LIABILITY WHETHER IN AN ACTION OF CONTRACT TORT OR OTHERWISE ARISING FROM OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE 960 Grid System Copyright c 2008 Nathan Smith http 960 gs 63 DIOPrScess ONTROL Permission is hereby granted free of charge to any person obtaining a copy of this software and associated documentation files the Software to deal in the Software without restriction including without limitation the rights to use
3. If it is considered that each feeding is followed by a discharging step where the amount of digested sludge removed from the reactor is equal to the added organic material then it is not necessary to insert any information in this section In this case the Discharging function Hide can be activated during the experiment 41 bIOpro ess ONTRO e Edit account Sign out D O D r L C e S S Instrument status Online CONTRO I OIC Feeding Discharging History Line 1 Line 2 Line 3 Line 4 Line 5 Line 6 Type Time Amount Concentration OLR HRT a ma g Liday day Feed 2013 07 10 11 00 67 6 957 3 00 29 05 Apply Delete Feed 2013 07 09 11 00 67 9 957 3 00 29 85 Apply Delete Feed 2013 07 08 11 00 67 6 957 0 60 149 25 Apply Delete Feed 2013 07 03 11 00 67 8 957 3 00 29 85 Apply Delete Feed 2013 07 02 11 00 67 8 957 3 00 29 85 Apply Delete Feed 2013 07 01 11 00 67 6 957 1 00 69 55 Apply Delete Feed 2013 06 28 11 00 67 8 957 3 00 29 85 Apply Delete Feed 2013 06 27 11 00 67 8 957 3 00 29 85 Apply Delete History Through this function the user can have access to the history of each reactor The previous feedings and discharges can be seen with regard to the time of feeding discharging amount and substrate concentration The corresponding parameters ORL and HRT are also calculated and displayed for each particular feeding The historic information from the reactors can be accessed by pressing the button Line x where 1 lt x
4. Symbol unit F g Fedamount pw raw V L e day Time for feeding j Specific Gas Production SGP The specific gas production SGP is calculated every time a report is generated The normalisation period e g h day may be selected G Manual and automatic mode SGP 7 Symbol unit G l day VD Organic Gas Yield Yorg The Organic Gas Yield Y or is calculated for the report based on the selected time units by only using the interpolated averaged values Manual and automatic mode Symbol units Raw average SGP NI I day Specific gas production interpolated value Average OLR gVS I day Organic loading rate interpolated value Wet Gas Yield Y yet The Wet Gas Yield Y we is calculated for the report based on the selected time using the interpolated averaged values units by only G Manual and automatic mode Ywet T Symbol units G Nl day Gas flow rate average value F g Fed amount average value 60 DIOPrScess ONTROL APPENDIX C LICENSES FOR THE OPEN SOURCE SOFTWARE ON BRS Not all software on the BRS is licensed in a way that allows copying modification distribution etc This chapter should not be seen as granting any extra permission not demanded by the original license Only the software specified in this chapter adheres to the specific licenses listed Mongoose Web Server MIT License Copyright c 2004 2010 Sergey Lyubka Permission is hereby
5. d Press Sign in 36 HOME Edit account Sign out O D y A C e S S Instrument status Online COUNT mM UL Expenmernt Feeding Discharging Biogas Reactor Simulator BRS Available information User manual Specifications Product sheet Copyright 2012 Bioprocess Control Sweden AB All rights reserved The web interface 1s organized in a number of pages all reachable from the start page named Home which is the first page the user enters Documentation and instructions regarding the BRS is available on the page The pages are organised in the order an experiment is setup executed monitored and finally documented The following pages are available Home The home page also contains links to the right to different kinds of information such as user manual technical specification and product information sheets Experiment The user prepares an experiment by setting up individual data for each reactor line Feeding Discharging The user has the possibility to specify type of substrate its concentration and schedule the time for feeding discharging and one of the following variable inputs amount requested OLR or HRT Control The user can start pause and end an experiment The user can also control the speed of the motors driving the reactor stirrers 37 bloprccess ONTROL Graphs Two graphs showing the accumulated gas flow rate and OLR amp HRT vs time are displayed Reports The user ca
6. BioReactor Simulator Operation and Maintenance Manual BRS BioReactor Simulator Release History Version 2 0 December 2014 Any questions related to this Operation and Maintenance manual should be directed to Bioprocess Control Sweden AB Scheelev gen 22 SE 223 63 Lund Sweden Tel 46 0 46 163950 Fax 46 0 46 163959 E mail info bioprocesscontrol com or support bioprocesscontrol com Web www bioprocesscontrol com This document contains proprietary information that is protected by copyright All rights are reserved No part of this publication may be reproduced in any form whatsoever or translated into any language without the prior written permission of Bioprocess Control Sweden AB Copyright O 2009 2013 Bioprocess Control Sweden AB Produced in Sweden TABLE OF CONTENTS LPREFACE 0 li l 2 GENERAL INFORMA TION coup tices 2 2 1 Conventional Continuous Fermentation Test cccccccceceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeees 2 2 2 Advantages of BRS over Conventional Fermentation Tests cccccccnnnnnnooonananancncnnnonnnoss 3 3 DELIVERY CHECKS ia A a a 4 4 PRERCOMMISSIONING 0 AAA 6 5 QUALITY RULES AND RECOMMENDATIONS coooccccccccccccconocincnononnnonnnncnnnnnnnnnncncnnnnnnns I 6 SAFETY CONSIDERATIONS 000 o is 8 7 EQUIPMENT DESCRIPTION DESIGN FUNCTION ooooooooocccccccncccccccnononananananonnnoncnnnos 9 TAs Sample Incubadora tiara 10 725 Gas Volume Me asu DEVICE cdas 11 Ly Bioreactor AC
7. During the upgrade process make sure that the system is not restarted or otherwise loses power This might result in a failed upgrade If this happens redo the entire upgrade process from the start It is recommended to leave the BRS to finish its upgrade for five minutes to ensure that everything has been installed properly When the upgrade is completed the system will restart with all previous data and settings intact and ready for use To verify that the upgrade has taken place go to the system tab of the system and in the right hand side compare the stated software version number to the version number of the file issued by BPC 52 DIOPrScess ONTROL 10 MAINTENANCE CLEAN THE EQUIPMENT REACTORS Use a bottlebrush and washing up liquid detergent to clean the inside and outside of the reactors Rinse well with water Do NOT autoclave the stirrers because they contain plastic and rubber parts which are characterised by uncertain heat stability and are therefore not suitable for autoclaving THERMOSTATIC WATER BATH If normal tap water is used calcareous deposits may appear on the reactors in the water bath and on the heating element Periodical cleaning of the inside of the tank and the heating element with a solution of 10 to 20 hydrochloric acid or a solution of water and vinegar acetic acid is advised Rinse with clean water afterwards For cleaning of the different parts use the following products 1 alcohol for
8. Local machine web interface Username user Password bpc Cloud web interface Username user provided email address Password bioprocesscontrol 9 2 FIRST TIME CONNECTING AND SETTING UP THE NETWORK AUTOMATIC CONFIGURATION The BRS system comes preconfigured for use with DHCP Dynamic Host Configuration Protocol and NTP Network Time Protocol meaning that if the network environment it is to be connected to supports these features no manual configuration of the system is required a Connect the shielded Ethernet cable to the gas volume measuring device Unit BRS B b Connect the shielded Ethernet cable to the internal network or other suitable network equipment Important Make sure that the network cable is connected prior to connecting the power c Connect the motor module s DC plug to the Unit BRS B d Connect the power supply 12 V DC first to the motor module and then to a standard 100 240 V 50 60 Hz power socket Important Make sure that the motor module s DC plug that supports the Unit BRS B with power 1s already connected before connecting the power adapter 30 bloprccess ROL Once the unit is powered on it will automatically acquire the needed information and connect to the BPC cloud solution within a few minutes Please note that in order to function properly the BRS system needs to have access to an internet connection To access the web interface of the system enter the following address please
9. integrated system Measuring range 10 to 4000 ml per hour Measuring range for instant gas flow rate 10 to 120 ml per minute Measuring resolution 10 0 1 ml Power consumption 15 W average 28 W maximum Dimension 51x44x18 cm Accuracy 5 RE amp Precision 1 CV MOTOR MODULE Dimensions 5 5x3x2 5 cm Weight approximately 50 g SOFTWARE Web based software running on cloud solution Accessibility from any location with an internet connection via industry strength encryption Support both for running feeding and discharging in manual or automatic mode Possibility for running experiments with high quantity of data generation Possibility for measuring both produced biogas and biomethane Real time gas flow rate HRT and OLR display Assisted SGP calculation Real time gas flow and volume normalisation Possibility for multiplexing allowing for simultaneous analysis initiation of parallel experiments at different startup times Standardised report generation in Microsoft Excel format for easy usage On line system logger for operational diagnosis 20 bioprcocess CONTROL 8 OPERATION In this section references are given to pictures in the Chapter 7 Equipment Description Design Function in the form of a letter and number within parenthesis e g A1 Please also refer user manual for the thermostatic water bath before installation and use 8 1 BEFORE START UP REACTORS a b c d e Perfo
10. save the report in the cloud software and if necessary further on in a desired folder on the local computer used to visualize the software interface See also Appendix and Chapter 9 BRS Software for details regarding data handling The report files can be generated anytime during the experiment and can be used for further data analysis and curve plotting in Microsoft Excel or other software capable of reading this file format The name of the data file is automatically generated consisting of the actual date and time For example a file saved on 1 of October 2012 at 12 30 will get the name report 20121001 1230 049 xls All data 1s adjusted to a user defined sampling time interval 1 e day hour or quarter of hour and it is generated in a Microsoft Excel file containing four sheets Process Data Process Parameters Feed Data and Discharge Data The following data are stored in the files 1 In the Process Data sheet a Time dates and times since the start of the experiment Flow rate Nl day Organic feeding gVS day Feeding g day Digester volume ml mono Ss Process temperature C g Process operation mode manual or automatic 2 Inthe Process Parameters sheet a Time dates and times since the start of the experiment b OLR gVS I day c HRT days d SGP NI I day e Organic yield NI gVS f Yield NI kg 3 In the Feed Data sheet a Time h days since the start of the experiment b Substra
11. 04 05 2014 04 05 2014 04 06 2014 04 06 2014 04 07 2014 04 07 2014 04 08 2014 04 09 16 46 06 40 20 33 10 26 09 20 14 13 04 06 18 09 07 53 21 46 11 40 91 33 Copyright 2013 Bioprocess Control Sweden AB All rights reserved a Above the graph window there are two buttons available Last week and Zoom out 1 Last week button adjusts the graph window to display the previous week data in an overview 11 Zoom out button adjusts the graph window so that more data is being displayed This button can be pressed several times to zoom out several steps 46 bIOpro ess ONTRO Lines to display in graph Gas production evaluation Y Y Y Y Y Y SGP NU L day 1 223 1 118 1 497 1 437 1 238 1 475 a E O O E tee Duration days 22 95 22 95 22 95 15 77 22 95 22 95 Reset Reset Reset Reset Reset Reset Flow rate NL day Last week Zoom out 2014 03 13 2014 03 16 2014 03 19 2014 03 22 2014 03 26 2014 03 28 2014 03 31 2014 04 03 2014 04 08 2014 04 09 23 33 21 00 18 26 15 53 13 20 10 46 09 13 06 49 04 06 01 33 OLR HRT g L day days 800 400 400 200 200 0 A s A a 9 2014 03 19 2014 03 18 2014 03 19 2014 03 22 2014 03 25 2014 03 28 2014 03 31 2014 04 09 2014 04 06 2014 04 09 23 33 21 00 18 26 15 53 13 20 10 46 09 13 06 49 04 06 01 33 Copyright 2013 Bioprocess Control Sweden AB All rights reserved In order to navigate pan the right mouse button is used right click and hold an
12. 4 TCP IPv4 and click on the button marked Properties g Write down the initial settings e g IP address subnet mask h Select Use the following IP address and enter the following values in the fields 1 IP address 192 168 10 10 2 Subnet mask 255 255 255 0 1 Select OR j Click Close in the remaining window WINDOWS XP Local Area Connection Properties wal internet Protocol TCP IP Properties Gereral Advanced General Correct ug You can get IP settings atsigned atomaticaly d pour network supports thes capabaty Dlhenmoe you need to atk your network admermtratos for B Reatek ATLOTOCE ATLETOSE Fad Configure the approgaate IP settings This connection utes the lollowang teens O Obtain an IP address automatically Use the bolong IP acters Y File and Panter Shanng lor Microsoft Networks IP addes Y QoS Packet Scheduler Y Y Interet Protocol TCPAP Subnet mask Delak osteway Deterpton Akort your computer lo access tesources on a Microsol Use the following DNS server addresses retor Preferred ONS server 208 67 222 222 Abeerate ONS server 208 67 220 220 C Show icon in notification area when connected F Notily me when this conection has imied o no conmectivly Advanced A Cea a In the Windows Start Menu select Control Panel 33 bloprccess ROL b Select Network Connections c Right click on the appropriate network adapt
13. This License applies to any program or other work which contains a notice placed by the copyright holder saying it may be distributed under the terms of this General Public License The Program below refers to any such program or work and a work based on the Program means either the Program or any derivative work under copyright law that is to say a work containing the Program or a portion of it either verbatim or with modifications and or translated into another language Hereinafter translation is included without limitation in the term modification Each licensee is addressed as you 69 bloprccess ROL Activities other than copying distribution and modification are not covered by this License they are outside its scope The act of running the Program is not restricted and the output from the Program is covered only if its contents constitute a work based on the Program independent of having been made by running the Program Whether that is true depends on what the Program does 1 You may copy and distribute verbatim copies of the Program s source code as you receive it in any medium provided that you conspicuously and appropriately publish on each copy an appropriate copyright notice and disclaimer of warranty keep intact all the notices that refer to this License and to the absence of any warranty and give any other recipients of the Program a copy of this License along with the Program You may charge a fee for t
14. and then to a standard 100 240 V 50 60 Hz power socket Wait approximately five minutes for the BRS to boot up and start the internal software Locate the pin sized hole on the back of the unit marked Reset and gently insert a needle shaped object into the hole to press the reset switch located inside When the switch has been pressed properly a red light should turn on inside the unit for a short while indicating that the system is rebooting When the system is finished rebooting approximately two minutes a blue light should turn on to indicate that the system is up and running again Disconnect the power supply from the wall power socket When the following reset procedure has taken place the BRS system will now be configured to have 192 168 10 11 as its IP address and it will be ready for manual configuration of the network settings through a computer connected directly to the Unit BRS B Important Make sure the computer is not connected to a wireless network If possible temporarily completely disable the wireless capability a Connect the shielded Ethernet cable to the gas volume measuring device Unit BRS B b Connect the shielded Ethernet cable to a computer Important Make sure that the network cable is connected prior to connecting the power c Connect the power supply 12 V DC to a standard 100 240 V 50 60 Hz wall power socket WINDOWS 7 a In the Windows Start Menu select Control Panel
15. b In the Control Panel select Network and Internet c Select Network and Sharing Center 32 y Local Ases Connection Properties laternat Protocol Verson 4 TCP Properties Metwodang Serna Genera Connect wang You can get IP settings asagred autanancaly your network supports a 81110 e ts capebllty Otherwee you need to ack pour network admi tator SY Restek ATLE1620 8111D Family POHE Ggabt Ethemet a po ho L Corto Qotar an P adress automescaly Use the folowing P address The connection uses the infowing tems Y Me Chere tor Mcrsoh Networks Y 0005 Pocket Schede Y Ho and Perter Sharing for Moost Networks Y 4 beenet Protocol Version 6 TCP IP we A de Y 4 Lrk Layer Topology Discovery Mapper LO Driver Y a Lei Layer Topology Discovery Responder oda e Use the folowing DNS server addresses etal Prop Preferred DNS server 23 67 Descriptor Tararm tor Control Petscol tenet Protocol The delat wide area network protocol that prowdes COmmuncabon across Gverse rtercomnected networks Aternate DNS server 208 67 Valdete settings upon ext d On the left side of the window select Change adapter settings e Right click on the appropriate network adapter and select Properties Usually this is named Local Area Connection possibly followed by a number It is important that the adapter corresponding to the connected Ethernet cable is selected f Select Internet Protocol Version
16. conditions must be put in place in order to achieve an optimal degradation and maximisation of gas yield Continuous fermentation tests thus deliver the first useful information about the capabilities and loading limits of a process which is essential for designing and operating a biogas plant as well as for creating models concerning the economical feasibility of a project Conventional continuous fermentation tests are laboratory scale methods subject to large variations not only due to the heterogeneous nature of bio wastes and bacteria culture used but also due to differences in experiment setup and non unified test protocols For example reactor configuration instrumentation and operational modes can all differ from one laboratory to another In addition the presentation of the results is not standardised either which makes comparability between two tests very difficult Furthermore the execution of a fermentation test in continuous mode is often a complex and very labour intensive and time consuming procedure spanning a considerable period of time The BRS has been developed by BPC for the on line monitoring and control of anaerobic fermentation tests in a continuous mode with modern instrumentation and IT technologies The DIOPrScess ONTROL gas flow rate measurements and data logging of the BRS are fully automated during the incubation period The high precision of gas flow monitoring is based on a patented flow meter array using t
17. copy modify merge publish distribute sublicense and or sell copies of the Software and to permit persons to whom the Software 1s furnished to do so subject to the following conditions e The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software e THE SOFTWARE IS PROVIDED AS IS WITHOUT WARRANTY OF ANY KIND EXPRESS OR IMPLIED INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM DAMAGES OR OTHER LIABILITY WHETHER IN AN ACTION OF CONTRACT TORT OR OTHERWISE ARISING FROM OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE jQuery flot Copyright c 2007 2009 IOLA and Ole Laursen Permission is hereby granted free of charge to any person obtaining a copy of this software and associated documentation files the Software to deal in the Software without restriction including without limitation the rights to use copy modify merge publish distribute sublicense and or sell copies of the Software and to permit persons to whom the Software 1s furnished to do so subject to the following conditions e The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software e THE SOFTWARE IS PROVIDED AS IS WITHOUT WARRANTY OF ANY KIN
18. difference in the amount of sample after drying and burning Mbgurnea and the initial amount of sample m TS Dried Wet VS Mpried Burned Mwy et A template for automatic calculation of the two parameters can be provided by BPC Please contact BPC in order to get more information BRS as a laboratory platform for simulating the operation of a full scale digester The kits containing 2 liter continuous stirred tank reactors CSTR 6 reactors kit can serve as a laboratory platform for simulating anaerobic degradation processes in a continuous mode of operation The reactors are equipped with three ports two of them for feeding the substrate 1 e feedstock discharging the digested sludge and the last one allowing for continuous measurements of the pH or other variables during the fermentation process 55 DIOPrScess ONTROL Helical coupling Outlet i Port for o H sensor i p df DA e e MODE OF OPERATION For the simulation of a full scale CSTR digester in a laboratory the inlet and the outlet ports of the 2 liter reactors should be closed with the help of tubing clamps or two way valves attached to the system through silicone tubing Important The clamps two way valves and silicone tubing are not delivered together with the system The feeding of the substrate can be performed either manually by a syringe or automatically with the help of a pump In the first case when the reactor i
19. from time to time issue software updates that can be installed on the system through an easy procedure These updates can contain both new features and bug fixes so it is imperative that when an update is received the system is updated at an appropriate time While there are no known issues with installing an upgrade while an experiment is underway it is recommended to wait for a running experiment to finish before applying an upgrade unless other reasons make an upgrade warranted In order to install an update issued by BPC append upgrade after the BRS local IP In order to ascertain the local IP of a specific BRS system login to the web user interface and go to the System tab Located under Instrument status there is a field called IP address An example of a url used to reach the upgrade page of an instrument would thus be http 192 168 10 11 app html upgrade Click on the Choose File button and select the zip file sent by BPC Note Do not unzip this file and never make any changes to the files contained inside of it Also make sure that the filename does not include any parentheses Some operating systems such as Microsoft Windows will sometimes append parentheses to filenames when the same file is downloaded more than once If this is the case remove the parentheses from the filenames before using it to upgrade the system When the file has been properly selected click on the Upgrade software button
20. granted free of charge to any person obtaining a copy of this software and associated documentation files the Software to deal in the Software without restriction including without limitation the rights to use copy modify merge publish distribute sublicense and or sell copies of the Software and to permit persons to whom the Software 1s furnished to do so subject to the following conditions e The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software e THE SOFTWARE IS PROVIDED AS IS WITHOUT WARRANTY OF ANY KIND EXPRESS OR IMPLIED INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM DAMAGES OR OTHER LIABILITY WHETHER IN AN ACTION OF CONTRACT TORT OR OTHERWISE ARISING FROM OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE Query tmpl Query BlockUI Copyright c 2011 John Resig http jquery com Permission is hereby granted free of charge to any person obtaining a copy of this software and associated documentation files the Software to deal in the Software without restriction including without limitation the rights to use copy modify merge publish distribute sublicense and or sell copies of the Software and to permit persons to whom the Software is furnished
21. lt 6 one by one The user has the possibility to correct edit or delete data regarding previous feedings and discharges 42 CONTROL DIOPrScess TROL Motor control Speed adjustment 80 Mixers on time min 1 Mixers off time min O Motor On off Apply Copyright 2012 Bioprocess Control Sweden AB All rights reserved Experiment Feeding Discharging Line control Line id Line 1 Line 2 Line 3 Line 4 Line 5 Line 6 A B Control 06668 Status Running Running Running Running Running Running DIOP Control roec O N T 71 Edit account Sign out Instrument status Online Graphs Reports This section handles the Control page The page shows different controls that can be changed by the user Motor Control Through this function the user can control the speed and the mixer on off time The motor status is either on or off To activate the settings the button Apply has to be pressed The settings are the same for all motors Important The motor speed should only be set at a value which is equal or higher than 80 to prolong the life of the motors 43 blopre if fD oN N Line id Control Status Line 1 Ex1 o stopped Line id Control Status Line 1 Ex1 D Running Line id Control Status Line 1 Ex1 O Paused Line control Each line has a set of buttons that control the experiment The first 1s the start exper
22. no 751 time 1319018 temp 22 3 pressure 100 9 2012 09 13 21 48 13 0200 sampler sensor 4 no 875 time 1319095 temp 22 3 pressure 101 0 2012 09 13 21 50 47 0200 sampler sensor 6 no 957 time 1319225 temp 22 3 pressure 101 0 2012 09 13 21 50 48 0200 sampler sensor 5 no 1015 time 1319242 temp 22 3 pressure 100 9 2012 09 13 21 53 38 0200 sampler sensor 23 no 951 time 1319425 temp 22 3 pressure 100 9 ha This section shows what the System page looks like The page also gives the user the possibility to log the status latest actions of the system Cell volumes Each flow cell has a unique volume When the cells are manufactured the flow cells are calibrated and the volumes are pre saved When an experiment is started the user of the BRS should check that the calibration values are correct in order to find out 1f they were changed to default values 1 e 10 00 Instrument status The last time when the system registered any data is displayed together with the local IP address of the instrument and the current version of the software The local IP address of the system can be used if there is a need to access the local web interface of the system used for changing network settings time settings and installing updates to the local software 50 DIOPrScess ONTROL System log The system log provides the user with an updated view of what the BRS unit logging software has registered This includes general events suc
23. note the s in https indicating secure encryption in the address bar of a web browser running on a computer connected to the internet https brs bioprocesscontrol com Important When the system is connected for the first time 1t might take up to 15 minutes before the system registers a connection in the BRS cloud software connection state can be seen in the top right corner and in the System page on the last seen section of the cloud interface This situation is absolutely normal and it should not be a cause for concern bioprcess L Welcome to Bioprocess BRS web application Email Password Remember me F Sign in When presented with the login page for the BRS web application enter the login information provided with the system and press the button marked Sign in MANUAL CONFIGURATION If the setup of the network where the BRS system is to be used does not support DHCP or for some other reason it is desirable to not use DHCP the BRS can be manually configured instead First 1t is required to reset the system from the default shipping configuration into a predefined fixed configuration This is achieved by the following steps a Connect the motor module s DC plug to the Unit BRS B The BRS communicates with the internet using port 80 data transfer and port 123 time synchronization 31 b c d e g DIOPrScess ONTROL Connect the power supply 12 V DC first to the motor module
24. with the product should be used The product contains alkaline AA batteries that need to be handled accordingly BPC reserves the right to correct any possible text and image errors as well as changes to technical data in this manual BEFORE GETTING STARTED Read this manual and additional separate manuals for the individual instruments before installing and using the equipment Keep the instruction manual for future reference and make sure it is easily available for people who regularly use the BRS Read the Bioreactor Agitation Systems instruction manual before using the equipment DIOPrScess ONTROL 6 SAFETY CONSIDERATIONS Using the BRS should always be done in an environment with good ventilation preferably in a laboratory fume hood When handling NaOH always use safety glasses a lab coat and plastic gloves See the safety data sheet for your chemicals for further information Always be cautious when handling electrical devices close to water The Agitation System contains rotating parts Make sure to tie back any loose hanging objects like clothing or hair when using the instrument The power adapter for the multifunction brushless DC Agitation System must never be used in the BRS detection unit DIOPrScess ONTROL 7 EQUIPMENT DESCRIPTION DESIGN FUNCTION With the BRS data recording and analysis are fully automated during the long fermentation period which significantly reduces time and labor demands High qual
25. 3 Delet report_20120810 0839 027 xIs 3 11 MB 2012 08 10 10 41 Delete gt report_20120810_0836_489 xls 826 KB 2012 08 10 10 38 a report_20120810_0812 457 xls 66 5 KB 2012 08 10 10 13 Delete report_20120809 0810_186 xls 821 KB 2012 08 09 10 11 Delete PT ey report 20120809 0724 946 xls 66 5 KB 2012 08 09 09 25 Delete report_20120808_0827_263 xls 3 07 MB 2012 08 08 10 30 report_20120807_0823_453 xls 3 05 MB 2012 08 07 10 25 ae report_ 20120807 0742 613 xls 790 KB 2012 08 07 09 43 Delete report_ 20120807 0702 161 xls 64 5 KB 2012 08 07 09 04 Delete lla id The reports generated by the user are stored on the cloud software to a maximum capacity of 20 MB The maximum storage capacity can be increased upon request The reports are saved as Excel files with typical names report date time random code 49 bIOprocess ON SYSTEM e Edit account Sign out IO D r ce S S instrument status Online GON CERO E Cell volumes mL Instrument status Cell 1 Cell 2 Cell 3 Last seen 1 minute ago 10 00 10 00 10 00 IP address 10 15 14 248 Cell4 Cell 5 Cell 6 Current version 3 0 v0 08 10 00 10 00 10 00 Apply Restore factory defaults System log Hide t 2012 09 13 21 24 30 0200 sampler sensor 2 no 749 time 1317683 temp 22 3 pressure 101 0 a 2012 09 13 21 25 56 0200 sampler sensor 3 no 947 time 1317763 temp 22 3 pressure 100 9 012 09 13 21 28 11 0200 sampler sensor 6 no 954 time 1317869 temp 22 3 pressure 101 0 2012 0
26. 550 C for 2 hours should be done in order to burn the organic matter The weight difference between the sample after heating at 105 C and 550 C reflects the VS content of the biomass The next three steps are usually followed to determine the TS and VS of a target sample 1 Preparation a Heat a dish to 550 C for 1 h b Place the dish in a desiccator for cooling 2 TS determination a Weigh the dish and record this value b Add 2 3 ml of a representative sample into the dish c Place the dish with the sample in an oven preheated to 105 C and allow the volatiles to evaporate for 20 h SAMPLE MM wart DIOPrScess ONTROL 3 VS determination a Take the dish out of the oven and allow it to cool to room temperature RT in a desiccator b Weigh the dish and record this value c Transfer this dish into a furnace pre heated to 550 C ignition d After 2 h take the dish out of the furnace and cool it to RT in a desiccator e Weigh the dish and record this value ignition 550 C gt Peg 2h tE SAMPLE m Dried Meurned TS is calculated as the ratio between the amount of dried sample mp ieq and the initial amount of wet sample Mwet VS content provides an estimation of the organic material in the sample and it is expressed either as the percent of the TS or as the percent of the wet sample the second case being applied in the BPC protocol when VS is calculated as the ratio between the
27. 9 13 21 28 17 0200 sampler sensor 4 no 871 time 1317899 temp 22 3 pressure 101 0 2012 09 13 21 30 07 40200 sampler sensor 5 no 1012 time 1318001 temp 22 3 pressure 100 9 2012 09 13 21 32 49 0200 sampler sensor 3 no 948 time 1318176 temp 22 3 pressure 101 0 2012 09 13 21 32 59 0200 sampler sensor 1 no 13669 time 6591013 temp 22 3 pressure 100 9 2012 09 13 21 34 00 0200 sampler sensor 4 no 072 time 1310242 temp 22 3 pressure 100 9 2012 09 13 21 35 42 0200 sampler sensor 6 no 955 time 1318320 temp 22 3 pressure 100 9 2012 09 13 21 35 45 0200 sampler sensor 2 no 750 time 1318358 temp 22 3 pressure 100 9 2012 09 13 21 37 11 0200 sampler sensor 5 no 1013 time 1318425 temp 22 4 pressure 101 0 2012 09 13 21 37 28 0200 sampler sensor 4 no 973 time 1318450 temp 22 4 pressure 101 0 2012 09 13 21 39 54 40200 sampler sensor 3 no 949 time 1318601 temp 22 3 pressure 100 9 2012 09 13 21 43 18 0200 sampler sensor 6 no 956 time 1318776 temp 22 3 pressure 100 9 2012 09 13 21 43 58 0200 sampler sensor 4 no 874 time 1318840 temp 22 3 pressure 100 2012 09 13 21 44 08 0200 sampler sensor 5 no 1014 time 1318842 temp 22 3 pressure 100 9 2012 09 13 21 44 28 0200 sampler sensor 1 no 13670 time 6591702 tenp 22 3 pressure 101 0 2012 09 13 21 46 44 0200 sampler sensor 3 no 950 time 1319011 temp 22 3 pressure 101 0 2012 09 13 21 46 45 0200 sampler sensor 2
28. ARTICULAR PURPOSE AND NONINFRINGEMENT IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM DAMAGES OR OTHER LIABILITY WHETHER IN AN ACTION OF CONTRACT TORT OR OTHERWISE ARISING FROM OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE jQuery IP Address jQuery Timer The MIT License Copyright c lt year gt lt copyright holders gt Permission is hereby granted free of charge to any person obtaining a copy of this software and associated documentation files the Software to deal in the Software without restriction including without limitation the rights to use copy modify merge publish distribute sublicense and or sell copies of the Software and to permit persons to whom the Software 1s furnished to do so subject to the following conditions 62 DIOPrScess ONTROL The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software THE SOFTWARE IS PROVIDED AS IS WITHOUT WARRANTY OF ANY KIND EXPRESS OR IMPLIED INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM DAMAGES OR OTHER LIABILITY WHETHER IN AN ACTION OF CONTRACT TORT OR OTHERWISE ARISING FROM OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE Downloadify
29. ASO id 13 e e e cs o A a sida a ational aaauacabaaduces 20 BOP ERA TITON sucia A Ai Deca Steeles 21 A O A ee ame eee Oe 21 SL A dl hans 25 So MONON Dioner a eae eee aaa eee 26 he E INL OOS 010 10 ea R EE EEN 27 9 BRS SOFTWARE cuotas ii 30 Dl Us rames and Pass W Ors ii IS ic 30 9 2 First Time Connecting and Setting up the Network oocccccccccccccccnnnonononnonannnncncnononos 30 DOs PUncion and Opera ON 36 0 MAINTENANCE coa 53 APPENDIX A FERMENTATION IN CONTINUOUS MODE METHOD DESCRIPTION 54 APPENDIX B MATHEMATICAL CALCULATIONS IN BRS SOFTWARE cccccccccccccccccccccccccceeeees 59 APPENDIX C LICENSES FOR THE OPEN SOURCE SOFTWARE ON BRS c cccccceeeeeeeettttteeees 61 DIOPrOcess ONTROL 1 PREFACE Bioprocess Control Sweden AB Bioprocess Control Sweden AB BPC is a technology and market leader in the area of advanced instrumentation amp control technologies for research and commercial applications in the biogas industry The company was founded in 2006 and today brings to market more than 15 years of industry leading research in the area of instrumentation control and automation of anaerobic digestion processes In 2009 BPC launched the Automatic Methane Potential Test System AMPTS a revolutionary product in the area of on site lab equipment for methane potential analysis Today the second generation of the AMPTS AMPTS II is used by biogas researchers commercial ope
30. D EXPRESS OR IMPLIED INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM DAMAGES OR OTHER LIABILITY WHETHER IN AN ACTION OF CONTRACT TORT OR OTHERWISE ARISING FROM OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE Excanvas Apache License Version 2 0 January 2004 http www apache org licenses 64 bloprccess ROL TERMS AND CONDITIONS FOR USE REPRODUCTION AND DISTRIBUTION 1 Definitions License shall mean the terms and conditions for use reproduction and distribution as defined by Sections 1 through 9 of this document Licensor shall mean the copyright owner or entity authorized by the copyright owner that 1s granting the License Legal Entity shall mean the union of the acting entity and all other entities that control are controlled by or are under common control with that entity For the purposes of this definition control means 1 the power direct or indirect to cause the direction or management of such entity whether by contract or otherwise or 11 ownership of fifty percent 50 or more of the outstanding shares or 111 beneficial ownership of such entity You or Your shall mean an individual or Legal Entity exercising permissions granted by this License Source form shall mean the preferred form for making modif
31. ED TO LOSS OF DATA OR DATA BEING RENDERED INACCURATE OR LOSSES SUSTAINED BY YOU OR THIRD PARTIES OR A FAILURE OF THE PROGRAM TO OPERATE WITH ANY OTHER PROGRAMS EVEN IF SUCH HOLDER OR OTHER PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES END OF TERMS AND CONDITIONS Acquiring Source Code All the source code for the software mentioned above that stipulates that this condition needs to be met can be acquired from Bioprocess Control upon request to info bioprocesscontrol com 73
32. L 6 Trademarks This License does not grant permission to use the trade names trademarks service marks or product names of the Licensor except as required for reasonable and customary use in describing the origin of the Work and reproducing the content of the NOTICE file 7 Disclaimer of Warranty Unless required by applicable law or agreed to in writing Licensor provides the Work and each Contributor provides its Contributions on an AS IS BASIS WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND either express or implied including without limitation any warranties or conditions of TITLE NON INFRINGEMENT MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE You are solely responsible for determining the appropriateness of using or redistributing the Work and assume any risks associated with Your exercise of permissions under this License 8 Limitation of Liability In no event and under no legal theory whether in tort including negligence contract or otherwise unless required by applicable law such as deliberate and grossly negligent acts or agreed to in writing shall any Contributor be liable to You for damages including any direct indirect special incidental or consequential damages of any character arising as a result of this License or out of the use or inability to use the Work including but not limited to damages for loss of goodwill work stoppage computer failure or malfunction or any and all other commercial damage
33. TWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE Busybox GNU GENERAL PUBLIC LICENSE Version 2 June 1991 Copyright C 1989 1991 Free Software Foundation Inc 51 Franklin Street Fifth Floor Boston MA 02110 1301 USA Everyone is permitted to copy and distribute verbatim copies of this license document but changing it is not allowed Preamble 68 bloprccess ROL The licenses for most software are designed to take away your freedom to share and change it By contrast the GNU General Public License is intended to guarantee your freedom to share and change free software to make sure the software is free for all its users This General Public License applies to most of the Free Software Foundation s software and to any other program whose authors commit to using it Some other Free Software Foundation software 1s covered by the GNU Lesser General Public License instead You can apply it to your programs too When we speak of free software we are referring to freedom not price Our General Public Licenses are designed to make sure that you have the freedom to distribute copies of free software and charge for this service if you wish that you receive source code or can get it if you want it that you can change the software or use pieces of it in new free programs and that you know you can do these things To protect your rights we need to make restrictions that forbid anyone to deny you these rights or to ask you to s
34. at least three years to give any third party for a charge no more than your cost of physically performing source distribution a complete machine readable copy of the corresponding source code to be distributed under the terms of Sections and 2 above on a medium customarily used for software interchange or c Accompany it with the information you received as to the offer to distribute corresponding source code This alternative is allowed only for noncommercial distribution and only if you received the program in object code or executable form with such an offer in accord with Subsection b above The source code for a work means the preferred form of the work for making modifications to it For an executable work complete source code means all the source code for all modules it contains plus any associated interface definition files plus the scripts used to control compilation and installation of the executable However as a special exception the source code distributed need not include anything that is normally distributed in either source or binary form with the major components compiler kernel and so on of the operating system on which the executable runs unless that component itself accompanies the executable If distribution of executable or object code is made by offering access to copy from a designated place then offering equivalent access to copy the source code from the same place counts as distribution of the source cod
35. ata is saved by pressing the button Apply The procedure is repeated for each particular line 39 bioprecess O N FEEDING DISCHARGING Edit account Sign out bioprccess emp i CONTROL Experiment Feeding Discharging Graphs Reports Feeding Discharging History Line 1 Line 2 Line 3 Line 4 Line 5 Line 6 a A B 0 D E F Feeding Running Running Running Running Running Running Mode of operation Manual Manual Manual Manual Automatic Automatic Substrate type milk waste milk waste sludge sludge food waste food waste 7 0 0 70 70 70 Substrate concentration 0 7 0 7 0 Type of unit w w VS VS YS YS VS VS Previous feeding time 2012 09 13 10 45 2012 09 13 10 46 2012 09 13 10 46 2012 09 13 10 46 2012 09 13 15 23 2012 09 13 15 23 Feeding interval h m 24 00 24 00 24 00 24 00 24 00 24 00 Time to next feeding 18 37 18 38 18 38 18 38 23 16 23 16 Feeding details Feeding time 2012 09 13 16 07 2012 09 13 16 07 2012 09 14 15 23 2012 09 14 15 23 Loading amount g 20 20 40 0 40 0 OLR 9VS Vday 3 13 3 14 HRT days 22 36 22 09 Apply Apply Apply Apply Apply Apply Discharging Show Copyright 2012 Bioprocess Control Sweden AB All rights reserved This page is used to control the substrate feeding and discharging of digested slurry The system Operation mode appears based on the selection at the previous page either as manual or automatic and it cannot be changed from this page Feeding The user has the poss
36. ave the ON OFF switch set to the OFF mode Connect one short motor cable to each motor excluding the last motor in the chain and then connect the free end of the cable from motor 1 to the free port on motor 2 and so on until motor 6 The Motor Controller should have the System ON OFF switch in the OFF mode Connect the long motor cable from motor 1 to the Motor Controller Connect the signal cable from the Motor Controller to the motor signal port on BRS Unit B WARNING The position of the signal and grounding terminals of the connectors are reversed on the Motor Controller compared to the AMPTS II system On the Motor Controller the ground is at the bottom terminal see the picture below Always make sure the proper cable is used and that it is inserted the correct way or the system could be permanently damaged 22 DIOPrScess ONTROL THERMOSTATIC WATER BATH Do not install or use the equipment before reading the separate instructions for the thermostatic water bath see above a After inserting all the reactors into the thermostatic water bath A5 fill it up with enough water to completely cover the equivalent height of the content in the reactors approx 8 liters If normal tap water is used calcareous deposits may appear in the bath and on the heating element It is therefore recommended to use deionised or distilled water b Place the plastic glass lid with 6 circular openings A4 on top of the water bath in order t
37. cessary protection should be used All work should be carried out inside a fume hood while wearing protective equipment see Chapter 6 Safety Considerations a Prepare a 3 M NaOH solution take into account that the 500 ml bottles should be filled with approximately 400 ml each Be careful and follow safety precautions as this is a highly alkaline solution After weighing the necessary amount of NaOH mix it with approximately of the required total volume of distilled water e g 120 g NaOH in of 23 b c d e DIOPrScess ONTROL 1 liter of water The heat generation following dissolution of NaOH in water is high so that adding small amounts of supplementary water followed by mixing 1s recommended When the NaOH is completely dissolved add the entire amount of remaining water and mix well Prepare a 0 4 Thymolphthalein pH indicator solution 40 mg in 9 ml ethanol 99 5 followed by the addition of 1 ml of water Thymolphthalein 1s insoluble in water but it is freely soluble in ethanol Prepare the NaOH solution containing the pH indicator by mixing 5 ml of the 0 4 Thymolphthalein solution per liter of 3M NaOH solution If all 6 reactors are used preparation of as much as 3 liters of NaOH and 15 ml of Thymolphthalein is recommended the additional solution may be necessary for quickly changing the fixation liquid if the solution becomes impaired the color changes from blue to colorless due to the pH indicator Thym
38. cleaning of stainless steel or alcohol with cotton duster for cleaning of plastic GAS VOLUME MEASURING DEVICE Rinse with water after use A soft brush can be used very gently if needed Make sure that all the flow cells are in the correct position The position can be checked by lifting the flow cell and making sure that the software registers the opening of the flow cell If CO was removed the used NaOH must NOT be poured into the sink It should be saved in dedicated vessels and disposed as hazardous waste See the safety data sheet for the chemicals for further information 53 DIOPrScess ONTROL APPENDIX A FERMENTATION IN CONTINOUS MODE METHOD DESCRIPTION This method description is based on the method used in the BPC laboratory It can be used as is used as a base for developing a new method or it can be replaced entirely by the customers own method Determination of Total TS and Volatile Solids VS Before starting any fermentation test in a continuous mode the biomass should be characterised with regard to total TS and volatiles solids VS The dry matter 1 e all inorganic and organic compounds is often expressed as TS and can be measured according to a standard protocol For a given biomass sample it is necessary to heat the sample up to 105 C in order to remove all water content VS is represented by the organic compounds in the sample After finishing the TS measurement heating the sample up to
39. d drag In order to zoom in the left mouse button is used to highlight a square area in the graph left click and hold and drag which is then displayed in the graph window in a zoomed state Gas production evaluation The specific gas production SGP is calculated and displayed for the period of time passed since the last reset of the calculations 47 bloprccess ONT REPORTS e edit account Sign out O p r CE S S Instrument status Online A Yast fan are o Sa S Experiment Feeding Discharging Generate Report Name report_20121009 1125 266 Interval Daily v Lines M1 M2 M3 M4 M5 M6 From 2012 09 09 To 2012 10 09 Create When pressing the New report button on the Reports page a new window will open containing a unique report name and where the frequency of the reporting period 1 e quarter of hour hour day the specific lines reactors from which the results should be included and the reporting period may be selected For generating the report the button Create should be pressed 48 bioprcocess CONTROL bioprccess oe RS See i Experiment Feeding Discharging New Reports frame CS E ER Stored reports Hew report e y CTI SIN Teema EE report_20120907 0945 344 xls 42 5 KB 2012 09 07 11 46 Delete report_20120830_0829_523 xls 19 KB 2012 08 30 10 30 Delete See report_20120830_0526 249 xls 30 5 KB 2012 08 30 10 29 Delete report 20120822 1312 605 xls 64 5 KB 2012 08 22 15 1
40. dation 10 If you wish to incorporate parts of the Program into other free programs whose distribution conditions are different write to the author to ask for permission For software which 1s copyrighted by the Free Software Foundation write to the Free Software Foundation we sometimes make exceptions for this Our decision will be guided by the two goals of preserving the free status of all derivatives of our free software and of promoting the sharing and reuse of software generally NO WARRANTY 11 BECAUSE THE PROGRAM IS LICENSED FREE OF CHARGE THERE IS NO WARRANTY FOR THE PROGRAM TO THE EXTENT PERMITTED BY APPLICABLE LAW EXCEPT WHEN OTHERWISE STATED IN WRITING THE COPYRIGHT HOLDERS AND OR OTHER PARTIES PROVIDE THE PROGRAM AS IS WITHOUT WARRANTY OF ANY KIND EITHER EXPRESSED OR IMPLIED INCLUDING BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE THE ENTIRE RISK AS TO THE QUALITY AND PERFORMANCE OF THE PROGRAM IS WITH YOU SHOULD THE PROGRAM PROVE DEFECTIVE YOU ASSUME THE COST OF ALL NECESSARY SERVICING REPAIR OR CORRECTION 12 IN NO EVENT UNLESS REQUIRED BY APPLICABLE LAW OR AGREED TO IN WRITING WILL ANY COPYRIGHT HOLDER OR ANY OTHER PARTY WHO MAY MODIFY AND OR REDISTRIBUTE THE PROGRAM AS PERMITTED ABOVE BE LIABLE TO YOU FOR DAMAGES INCLUDING ANY GENERAL SPECIAL INCIDENTAL OR CONSEQUENTIAL DAMAGES ARISING OUT OF THE USE OR INABILITY TO USE THE PROGRAM INCLUDING BUT NOT LIMIT
41. e even though third parties are not compelled to copy the source along with the object code 4 You may not copy modify sublicense or distribute the Program except as expressly provided under this License Any attempt otherwise to copy modify sublicense or distribute the Program is void and will automatically terminate your rights under this License However parties who have received copies or rights from you under this License will not have their licenses terminated so long as such parties remain in full compliance 5 You are not required to accept this License since you have not signed it However nothing else grants you permission to modify or distribute the Program or its derivative works These actions are prohibited by law if you do not accept this License Therefore by modifying or distributing the Program or any work based on the Program you indicate your acceptance of 71 bloprccess ROL this License to do so and all its terms and conditions for copying distributing or modifying the Program or works based on it 6 Each time you redistribute the Program or any work based on the Program the recipient automatically receives a license from the original licensor to copy distribute or modify the Program subject to these terms and conditions You may not impose any further restrictions on the recipients exercise of the rights granted herein You are not responsible for enforcing compliance by third parties to this Lic
42. eir Contribution s alone or by combination of their Contribution s with the Work to which such Contribution s was submitted If You institute patent litigation against any entity including a cross claim or counterclaim in a lawsuit alleging that the Work or a Contribution incorporated within the Work constitutes direct or contributory patent infringement then any patent licenses granted to You under this License for that Work shall terminate as of the date such litigation is filed 4 Redistribution You may reproduce and distribute copies of the Work or Derivative Works thereof in any medium with or without modifications and in Source or Object form provided that You meet the following conditions a You must give any other recipients of the Work or Derivative Works a copy of this License and b You must cause any modified files to carry prominent notices stating that You changed the files and c You must retain in the Source form of any Derivative Works that You distribute all copyright patent trademark and attribution notices from the Source form of the Work excluding those notices that do not pertain to any part of the Derivative Works and d If the Work includes a NOTICE text file as part of its distribution then any Derivative Works that You distribute must include a readable copy of the attribution notices contained within such NOTICE file excluding those notices that do not pertain to any part of the Derivative Works in a
43. ense 7 If as a consequence of a court judgment or allegation of patent infringement or for any other reason not limited to patent issues conditions are imposed on you whether by court order agreement or otherwise that contradict the conditions of this License they do not excuse you from the conditions of this License If you cannot distribute so as to satisfy simultaneously your obligations under this License and any other pertinent obligations then as a consequence you may not distribute the Program at all For example if a patent license would not permit royalty free redistribution of the Program by all those who receive copies directly or indirectly through you then the only way you could satisfy both it and this License would be to refrain entirely from distribution of the Program If any portion of this section is held invalid or unenforceable under any particular circumstance the balance of the section is intended to apply and the section as a whole 1s intended to apply in other circumstances It 1s not the purpose of this section to induce you to infringe any patents or other property right claims or to contest validity of any such claims this section has the sole purpose of protecting the integrity of the free software distribution system which is implemented by public license practices Many people have made generous contributions to the wide range of software distributed through that system in reliance on consistent applicatio
44. er and select Properties Usually this is named Local Area Connection possibly followed by a number It is important that the adapter corresponding to the connected Ethernet cable is selected d Select Internet Protocol and click on the button marked Properties e Write down the initial settings e g IP address subnet mask f Select Use the following IP address and enter the following values in the fields 1 1 IP address 192 168 10 10 1 2 Subnet mask 255 255 255 0 g Select OK h Select OK in the remaining window Show Al q Show All Q About This Mac Personal PAD Software Update ce Ed pai uy ry O Q App Store Appearance Desktop amp Dock Expos amp Language 4 Secunty Spotight a EN Screen Saver Sosces Text Se 5 Connected Dock gt g Er San n Location P p s amp AirPort gt Configure IPv4 Manually ODs 6 DVDs Ospreys Lre gr Leyooyd Mouse Trackpad Print amp Fax Sound Saver IP Address 192 168 10 12 Recent Items b internet amp Wireless Subset Mask 255 255 255 0 Force Quit XV PA a 3 Router Sleep Ce PRIN aren e DNS Server Restart ARD Search Domains Shut Down 244 0 a QU INIA Unrersal Log Out 02 Q Accounts Date amp Teme Paremal Software Speech Startup Disk Time Machi Cosmo Up ste tarthOesk id Click the lock 10 prevent further changes Assist me Revert Apply Select the Apple Menu button and select System Preferences a Select Network b Wri
45. ess operation is characterised by the parameters organic loading rate OLR solid retention time SRT and hydraulic retention time HRT which can be optimised with the help of the BRS in laboratory scale experiments ORGANIC LOADING RATE OLR The OLR is the quantity of the organic material e g VS for solid waste COD for waste waters fed per reactor volume on a daily basis and is expressed as gys Xday This parameter considers both the concentration and the amount of incoming substrate and is dependent on the reactor size thus representing a very good parameter for regulating the feeding of a reactor and at the same time assessing the performances of a reactor A recommended value to start with for a mesophilic process 35 39 C 1s normally around 2 3 Gvs lxday however processes should also be tested at higher levels of ORL The OLR gvs lxday 1s dependent on the amount F g and concentration C of the incoming substrate the active volume of the digester V 1 and can easily be calculated according to the equation below HYDRAULIC SOLIDS MICROORGANISM RETENTION TIMES HRT SRT MRT The retention time refers to the period a given material spends in the digester and is usually expressed in days HRT measures the length of time that a liquid remains in the system and is determined by dividing system volume by feedstock volume SRT is the time that feedstock solids remain in the system while MRT is the average time tha
46. funnel shaped feeding units with 6 silicone stoppers 6 bent glass discharging tubes 18 plastic screw caps 12 with and 6 without holes 12 silicone sealing rings 6 plastic tubing clamps UNIT BRS B 1 water bath package including water tank flow cell holder injection moulded flow cells containing magnetic metal pieces base and protection plate l plastic glass lid for the water tank including straight connector manual plastic water pump 1 power adapter input 100 240 V 50 60 Hz output 12 VDC 5A OTHER COMPONENTS 1 shielded Ethernet cord 1 box of 15 m flexible Tygon tubing 1 number markers kit for tubes 6 plastic lids blue l manual OPTIONAL EQUIPMENT Glass reactors 5 Stainless steel reactors 5 and 10 Gas sampling units Plastic screw caps with hole Glass bottles 0 5 or 1 1 Rubber stoppers with 2 metal tubes ONTROL bloprcocess 4 PRE COMMISSIONING The following items are not included in the delivered BRS however they will may be required to operate the BRS Silicone spray or other similar lubricant Silicone tubing two way valves and clamps Additional wall socket adapters plugs contacts the ones supplied are according to European US or UK standards depending on original purchase order If a customer has a gas chromatograph and 1s interested in off line biogas composition analysis gas sampling units can be ordered separately from BPC N or a mixture of N2 CO gt t
47. g the plastic motor support or the metallic screws holding the motor in place to avoid friction from the movement of the helical coupling 21 g bloprccess ONTROL Cut 6 pieces of Tygon tubing A9 of sufficient length to connect one of the small metal tubes on top of the rubber lids A2 of each reactor to the corresponding tubing connector on the connection block B4 located on the back of the detection unit Cut 6 pieces of Tygon tubing A10 at lengths of about 10 cm and connect them to each of the free small metal tubes on top of the rubber lids A2 of the reactors Close the tubing pieces with the help of plastic tubing clamps A11 h Equip the reactors with the inlet 1 e funnels or glass tubes A15 and outlet ports bent glass tubes A16 by inserting the glass tubes through the screw connector caps A13 adapted with the silicone sealing caps A12 If the third port is not used for pH measurements A14 it should be closed with a screw connector cap without a hole For creating an anaerobic environment the inlet and outlet ports should be closed with the help of tubing clamps or two way valves attached to the system through silicone tubing The clamps two way valves and the silicone tubing are not delivered together with the system MOTOR CONNECTIONS a b c d e Make sure that the power adapter for the Motor Controller is disconnected from the power supply The motor modules should all h
48. g to the principle of liquid displacement amp buoyancy and can monitor low gas flows a digital pulse is generated when a defined volume of gas flows through the device An integrated embedded data acquisition system 1s used to record display and analyse the results 7 1 SAMPLE INCUBATION UNIT sE n bropro ce Unit BRS A Al Multifunction Brushless DC motor A2 Rubber stopper with 2 metal tubes 1 plastic tube and rotating shaft for mixing A3 2 liter glass bottle reactor with 3 ports A4 Plastic lid for thermostatic water bath with circular openings for reactors A5 Thermostatic water bath A6 Helical coupling A7 Plastic screw thread cap motor holder A8 Bent stirring rod rotating shaft 10 7 2 GAS VOLUME MEASURING DEVICE DlOpr cess ROL Diore w r a bioprccess CONTROL Bl bioprcocess CONTROL B4 B5 B6 B7 Unit BRS B B1 Water bath package including water tank flow cell holder protection plate and electronics B2 Injection moulded flow cell B3 Manual plastic water pump B4 Connection block B5 Motor signal connection socket for the Motor Controller 12 V DC 3 A B6 Power connection socket for the motor module 12 V DC 5 A B7 Shielded Ethernet socket B8 Power adapter 12 VDC 5 A bloprccess ONTROL 7 3 BIOREACTOR AGITATION SYSTEM Two different bioreactor agitation systems are available from Bioprocess Control 1 an initial configuration in left picture based o
49. h as flow cell openings together with time temperature and pressure It also includes errors experienced by the various subsystems of the BRS When contacting BPC with support questions it is good to have an up to date copy of the system log as well as a copy from when a problem occurred at hand to be able to provide these upon request 51 DIOPrScess ONTROL CONNECTIVITY In order for the system to function properly it needs to have a reliable internet connection available If the internet connection is temporarily lost the system will cache the data locally but no new data will be accessible through the web user interface Once the internet connection of the system is restored all cached data will be uploaded with appropriate time stamps and will be made accessible While the system is capable of sustaining local data logging while experiencing both frequent and prolonged connection losses it is recommended to try to avoid having the system offline as much as possible As with all computer equipment care should be taken to ensure that the system functions after an unscheduled reboot Make sure that the experiment is running correctly and that data is being collected in an appropriate manner Also make sure that any other connected equipment such as the thermal water bath has restarted safely and with the correct settings SOFTWARE UPGRADE Care should always be taken to keep the built in software of the BRS up to date BPC will
50. hat the mixing works properly THERMOSTATIC WATER BATH a b Periodically check the water level in the thermostatic water bath Fill up with additional deionised or distilled water when necessary 26 DIOPrScess ONTROL GAS VOLUME MEASURING DEVICE a b c Check that the water level in the water bath for the gas flow measuring device is within the recommended range Fill up with additional deionised or distilled water to the recommended water level when necessary Make sure that data is registering properly REMOVING CO Ir NECESSARY a b The pH indicator Thymolphthalein will turn from blue to colourless when the CO binding capacity of the NaOH solution decreases below optimal At this point replacement of the bottle with NaOH solution is recommended for avoiding the CO gas to pass to the detection unit At 22 C about 20 25 1 CO can theoretically be captured in each bottle with 400 ml solution before it needs to be changed To change a NaOH solution bottle place the tubing clamps on the tubing on each side of the bottle and then disconnect the bottle by gently pulling the Tygon tubing Replace the old NaOH solution with a fresh one and open remove the clamps SOFTWARE See the Chapter 9 BRS Software for information regarding software function and operation 8 4 END OF OPERATION a b d e g h Note the total running time if this is of interest In the saved data files the
51. he physical act of transferring a copy and you may at your option offer warranty protection in exchange for a fee 2 You may modify your copy or copies of the Program or any portion of it thus forming a work based on the Program and copy and distribute such modifications or work under the terms of Section above provided that you also meet all of these conditions a You must cause the modified files to carry prominent notices stating that you changed the files and the date of any change b You must cause any work that you distribute or publish that in whole or in part contains or is derived from the Program or any part thereof to be licensed as a whole at no charge to all third parties under the terms of this License c If the modified program normally reads commands interactively when run you must cause it when started running for such interactive use in the most ordinary way to print or display an announcement including an appropriate copyright notice and a notice that there is no watranty or else saying that you provide a warranty and that users may redistribute the program under these conditions and telling the user how to view a copy of this License Exception if the Program itself is interactive but does not normally print such an announcement your work based on the Program is not required to print an announcement These requirements apply to the modified work as a whole If identifiable sections of that work are not der
52. he principle of liquid displacement and buoyancy The organic loading rate OLR hydraulic retention time HRT and specific gas production SGP are calculated and displayed in real time Any type of reactor can be connected to the BRS through a gas inlet port However designing a gas tight reactor that allows for a high liquid and gas mass transfer as well as continuous mixing and heating is a difficult task and many systems are considered to be unreliable Therefore the BRS is available with a verified set of reactors and with several different configurations such as CSTR standard UASB EGSB IC Biofilm etc optional From a biogas producers point of view increasing the knowledge and experience of a digestion process improving the method of selecting optimal substrate feeds developing open and closed loop control strategies identifying stress factors in the degradation process and implementing adjusted reactor designs have a significant impact on the initial design as well as operational and economic details of a biogas plant 2 2 ADVANTAGES OF BRS OVER CONVENTIONAL FERMENTATION TESTS The BRS provides several unique functionalities that are important for anaerobic fermentation tests in a continuous mode of operation e User friendly interface both for experiment setup and follow up e Real time data logging of gas flow rate e Real time pressure and temperature compensation of gas flow rate measurements e Real time gas flo
53. hould be kept above 20 days 58 DIOPrScess ONTROL APPENDIX B MATHEMATICAL CALCULATIONS IN BRS SOFTWARE In this section some issues important for the calculation of process parameters during a fermentation test in continuous mode monitored by the BRS are presented In the text below the process parameters are given either as raw or average values 1 raw is the data that is logged in the system when the Apply button is pressed and 11 average value means the average of the parameter for the time basis that is used in the report file Organic Load O The organic load O is calculated every time a load is applied to the system This value is averaged and further used in the generated MS Excel report Manual and automatic mode 0 FxC Symbol unit Parameter F g C Concentration of feed Organic Loading Rate OLR The organic loading rate OLR is calculated every time a load is applied to the system This value is also averaged and used in the generated MS Excel report Manual mode Automatic mode Symbol unit F 2 r C Concentration of feed VD t day Time for feeding j raw Hydraulic Retention Time HRT The hydraulic retention time HRT is calculated every time a load is applied to the system This value is averaged and used in the MS Excel file which is generated from the BRS system 59 DIOP Manual mode HRT Vx t E tj 1 F V Automatic mode HRT z FOCESS ONTROL
54. ibility to specify the type of substrate and its concentration expressed in TS VS or COD units as w w The information related to the previous feeding time the feeding interval and the time to next feeding appears in this page If the time until the next feeding has already expired it will be marked in red otherwise it will be green Feeding details In this section the user will introduce the values for the feeding time and one of the interested parameters such as loading amount OLR or HRT Depending on which parameter is chosen the 40 bloprccess ROL other two are directly calculated to give the user the optimal support for how to feed the reactor The unit for the OLR is related to the previous characterisation of the substrate e g gVS I day gCOD day After introducing the values for one line the user has to press Apply in order to store the data The procedure is repeated for each individual reactor Discharging Hide Previous discharging time 2012 08 01 10 04 N A NA N A NZA NZA Discharging time Discharged amount g Apply Apply Apply Apply Apply Apply Discharging The user has the possibility to simultaneously view another section related to the discharging step by activating the hidden Discharging function Show The previous discharging time will be displayed and the user will be able to introduce the new discharging time and the discharged amount All data will be saved into the report generated
55. ications including but not limited to software source code documentation source and configuration files Object form shall mean any form resulting from mechanical transformation or translation of a Source form including but not limited to compiled object code generated documentation and conversions to other media types Work shall mean the work of authorship whether in Source or Object form made available under the License as indicated by a copyright notice that is included in or attached to the work an example is provided in the Appendix below Derivative Works shall mean any work whether in Source or Object form that is based on or derived from the Work and for which the editorial revisions annotations elaborations or other modifications represent as a whole an original work of authorship For the purposes of this License Derivative Works shall not include works that remain separable from or merely link or bind by name to the interfaces of the Work and Derivative Works thereof Contribution shall mean any work of authorship including the original version of the Work and any modifications or additions to that Work or Derivative Works thereof that is intentionally submitted to Licensor for inclusion in the Work by the copyright owner or by an individual or Legal Entity authorized to submit on behalf of the copyright owner For the purposes of this definition submitted means any form of electronic verbal or written c
56. iment button When hovering over 1t with the mouse and pressing 1t the text Are you sure This will erase all 1s displayed previous experiment data Important When pressing the Start experiment button a new session 1s started and all data from the previous session will be deleted An experiment may be paused at any time This is done by pressing the corresponding Pause button During pause no data 1s recorded but the time is still registered By pressing the Pause button again the experiment will continue normally When the Pause button has been pressed the End experiment button becomes available By pressing the End experiment button neither data nor time are further recorded The collected data is available until the next time the Start experiment is pressed After starting pausing stopping an experiment e g Line x the following message will appear at the top of the page Line x was successfully started paused stopped 44 bloprccess ONTROL GRAPH Lines to display in graph Gas production evaluation Y El Y Y v J SGP 1 247 2 072 1 18 1 53 1 052 1 358 mm A he NL day Duration days 5 96 5 96 5 96 5 96 5 98 5 98 Reset Reset Reset Reset Reset Reset 13108 1 2013 08 2013 08 13 2013 03 13 lt 3108 12 13 08 12 2013 E 20 13 08 13 Copyright 2013 Bioprocess Control Sweden AB All rights reserved This section shows what the Graphs page may look like In s
57. ing or disconnecting the Motor Controller or any of the motor cables When the system has been turned off and then switched back on 1t can take up to 8 seconds for it to start up again DIRECTION SWITCH The DIRECTION switch is located on the side of the motor and marked on the very top of the motor unit just above where the actual switch 1s located It is used to set the motor to rotate in a CW or a CCW direction Setting the switch to AUTO gives the Motor Controller automatic control over the reversal of the direction Below is a table that will show the output of the different combinations of switch settings on the Motor Controller and the motor unit Settings Mixer output Motor Controller Motor unit switch Intervals Rotation oe switch Starting and Constantly CW stopping according to Motor Controller intervals AUTO Starting and Reversing directions stopping according to Motor Controller intervals Starting and Constantly CCW stopping according to Motor Controller intervals Constantly CW ha oA The latest direction used before the Motor Controller AUTO switch was set to off er 18 bloprccess ONTROL RESET BUTTON If an error is encountered somewhere on the driver board in the motor unit a red LED will light up on the side of the motor unit opposite to where the switches are located A marking on the top of the motor unit shows where to find it see picture below The problems could be e g high
58. ity data regarding key process parameters e g gas flow rate gas yield organic loading rate OLR hydraulic retention time HRT specific gas production SGP etc can be used to extract information regarding the degradation process This will in turn allow for a much better understanding of a specific biomass substrate further leading to an improved process operation The BRS can be divided into two units BRS A and BRS B please refer to the photos included With BRS A sample incubation unit up to 6 reactors containing mixtures of a sample with anaerobic inoculum are incubated at a desired temperature The reactors are equipped with three ports a port for feeding the substrate 1 e feedstock a port for discharging the digested sludge and a port for the continuous measurement of pH or temperature during the fermentation process or insertion of other suitable probes The media in each reactor is mixed by means of a slowly rotating agitator Biogas is then continuously produced and registered by the system With the BRS both the OLR and HRT are calculated and presented in real time together with normalised gas flow For feeding high suspended solids a funnel shaped feeding device is recommended which can be ordered separately from BPC With unit BRS B gas volume measuring device the volume of gas released is measured using a wet gas flow measuring device with a multi flow cell arrangement This measurement device works accordin
59. ived from the Program and can be reasonably considered independent and separate works in themselves then this License and its terms do not apply to those sections when you distribute them as separate works But when you distribute the same sections as part of a whole which is a work based on the Program the distribution of the whole must be on the terms of this License whose permissions for other licensees extend to the entire whole and thus to each and every part regardless of who wrote it Thus it is not the intent of this section to claim rights or contest your rights to work written entirely by you rather the intent is to exercise the right to control the distribution of derivative or collective works based on the Program 70 bloprccess ROL In addition mere aggregation of another work not based on the Program with the Program or with a work based on the Program on a volume of a storage or distribution medium does not bring the other work under the scope of this License 3 You may copy and distribute the Program or a work based on it under Section 2 in object code or executable form under the terms of Sections and 2 above provided that you also do one of the following a Accompany it with the complete corresponding machine readable source code which must be distributed under the terms of Sections 1 and 2 above on a medium customarily used for software interchange or b Accompany it with a written offer valid for
60. last data collected will be from the last opening of a cell Generate a report in the Reports menu Download the report and open it to make sure that the report has been generated properly and that no errors occurred while downloading the file Stop the logging by pressing the pause button and then the stop button Note When pressing the stop button the experiment associated with that particular cell can no longer be continued Turn off the thermostatic water bath Set the ON OFF switch on each motor unit to OFF Set the System switch on the Motor Controller to OFF Unplug the power adapters for the Motor Controller and the detection unit from the power source Disconnect all tubing connections between the reactors and the gas volume measuring device If CQO elimination solution bottles have been used disconnect all tubing between the NaOH bottles and the reactors and detection unit BRS Unit B Empty the water from the water bath by using the manual plastic water pump only Do not pour out water from water bath in any other way Directly pouring out the water without using the water pump might allow water to flow into the space between the water tank and the stand as well as flow into the stand There 1s a potential risk of electronic 2 DIOPrScess ONTROL damage even if only very limited amounts of water are accidently in contact with the electronic circuit boards inside the stand DATA HANDLING When a report is generated
61. n generate a report from the page The report contains experiment data for each reactor line System This page contains the configuration of IP addresses and cell volume settings the system log and set date and time Performing a system reboot is also possible from this page 38 bioprecess O N EXPERIMENT instrument status Online S N TAROS E 0 O p e EE S S Edf account Sign out Process settings Process temperature C Reactor volume mL AU Apply Experiment settings Line 1 Line 2 Line 3 Line 4 Line 5 Line 6 Name of experiment A C D E Reactor active volume mL 2000 2000 2009 2000 2000 2000 Feeding interval hh mm 2400 24 00 240 24 00 24 00 24 00 Type of unit TSVS COD vS vS YS WS IS vS Mode of operation Marnus Manual Manual Manual Aulornati Automat Apply This page is used to setup an experiment The user starts by inserting the process settings and continues with the experiment parameters for each individual reactor Process settings Some settings are common for all cells for example the process temperature and the reactor volume Experiment settings The user enters the name of the experiment and relevant values for the active volume of the reactor the feeding interval and the unit for the substrate concentration 1 e TS VS COD The BRS allows the user to run the process in both manual and automatic feeding and discharging modes When the user has filled in the values for one line the d
62. n of that system it is up to the author donor to decide if he or she is willing to distribute software through any other system and a licensee cannot impose that choice This section is intended to make thoroughly clear what is believed to be a consequence of the rest of this License 8 If the distribution and or use of the Program is restricted in certain countries either by patents or by copyrighted interfaces the original copyright holder who places the Program under this License may add an explicit geographical distribution limitation excluding those countries so that distribution is permitted only in or among countries not thus excluded In such case this License incorporates the limitation as if written in the body of this License 9 The Free Software Foundation may publish revised and or new versions of the General Public License from time to time Such new versions will be similar in spirit to the present version but may differ in detail to address new problems or concerns Each version is given a distinguishing version number If the Program specifies a version number of this License which applies to it and any later version you have the option of following the 72 DIOPrScess ONTROL terms and conditions either of that version or of any later version published by the Free Software Foundation If the Program does not specify a version number of this License you may choose any version ever published by the Free Software Foun
63. n standard brush DC motors with a gearbox and 11 a multifunction version in right picture based on a unique design of brushless DC stepper motors The motors are shown on a glass bottle different from the type and size used in the BRS For a full description of each system please refer to the Bioreactor Agitation Systems manual The reasons for introducing a new agitation system are e To increase the life span of the system by changing to a brushless stepper DC motor based system for direct drive e To offer improved and more flexible mixing properties by adding new features o Reversal of motor direction clockwise CW counter clockwise CCW o Timer function to set time periods for the motor reversal o Remote speed control on a broad range 1 e 5 100 A photo of the multifunction configuration of the motor the brushless DC stepper motor is presented below The complete agitation system consists of the following components Motor Controller 24 V power adapter for the Motor Controller brushless steppers DC motor units with plastic caps helical couplings short motor cables 0 2 m long motor cable 1 5 m signal cable and a hex tool 13 MOTOR CONTROLLER The Motor Controller provides power to the motors via the motor cables and it is very important that the Motor Controller always is turned off and is unplugged from the power source when any cables are connected or disconnected from the motors Turning the switch off i
64. o minimise the evaporation of water during the experiment c It is recommended to place a thermometer through one of the holes in the lid inside the water bath in order to keep track of the real temperature of the reactors during the incubation period GAS VOLUME MEASURING DEVICE a Install Unit BRS B on a flat and stable surface Use appropriate instrumentation 1 e bubble level device to verify the horizontality of the surface and to adjust the level of the system placed on the surface b Fill up the water bath B1 with deionised or distilled water The water level should be within the limits of the marking on the water bath c Make sure to remove the protective film from the transparent lid of the water tank NETWORK AND POWER CABLES a Connect one end of the Ethernet cord to the gas volume measuring device BRS Unit B b Connect the other end of the Ethernet cord to an internal network or suitable network equipment For its operation the BRS system needs to have access to an internet connection c Make sure that the network cable is connected to the network prior to connecting the power supply ALKALINE SOLUTION OPTIONAL If CO elimination from the biogas is foreseen optional 500 ml bottles with rubber cap and 2 metal tubes can be purchased from BPC in this scope The biogas produced in each reactor will pass through individual bottles containing alkaline solution For the preparation of NaOH solution for CO gt fixation ne
65. o obtain anaerobic conditions during the start up phase of the experiment BPC recommends a mixture of Nz 60 and CO 40 to be used NaOH reagent grade 97 pellets e g Sigma Aldrich 221465 or equivalent quality a pH indicator such as thymolphthalein 2 2 Dimethyl 5 5 di 1so propyl phenol phthalein C23H3004 CAS 125 20 2 ACS reagent dye content 95 e g Sigma Aldrich 114553 or equivalent quality and ethanol ACS reagent 99 5 e g Sigma Aldrich 459844 or equivalent quality for the preparation of the alkaline solution for CO fixation if the user is interested in measuring bio methane DIOPrScess ONTROL 5 QUALITY RULES AND RECOMMENDATIONS The product guarantee provided corresponds to the guarantee stipulated on the confirmed product order form and shipping documentation The removal of the metal bottom plate on the Unit BRS B is considered a breach of guarantee For guarantee claims relating to the thermostatic water bath contact the manufacturer directly see separate handbooks and inform BPC When 2000 ml bottles are used as reactors the recommended headspace in the bottles during the methane potential test analysis is approximately 300 ml For a high accuracy analysis the BRS should not be exposed to mechanical vibrations and or high frequency radio transmissions and should be placed on a level and stable surface In order to guarantee the quality and performance of the product only the parts delivered
66. olphthalein Add approximately 400 ml of the mixture containing NaOH solution and Thymolphthalein pH indicator into each glass bottle 500 ml Lubricate rubber stoppers on the side that is in contact with the glass bottle preferably with silicone spray or silicone stick Place a rubber stopper with two metal tubes connected in the opening of each bottle seal by placing the plastic lid on the top and screw until the thread on the bottle is no longer visible and the lid is properly sealed 24 bloprccess ONTROL 8 2 START UP MOTOR AND POWER CABLES a Check that all motor cables are connected to each other and that the long motor cable is connected between the first motor and the Motor Controller b Check that the analogue signal cable is connected between the Motor Controller and BRS Unit B c Connect the 12 V power adapter to the detection unit BRS Unit B C6 and to a 100 240 V 50 60 Hz standard power socket After this connect the 24 V power adapter to the Motor Controller and to a 100 240 V 50 60 Hz standard power socket IMPORTANT Always connect power to the units in this order to minimize the risk of damage to the system Always make sure that the correct power supply is connected to the different units Failing to do so will result in damage See schematic picture below Detection Unit Motor Controller ue a i We SA WARNING Never connect the Power Adapter tor the Motor Controller to the Detecti
67. ome browsers the data may initially not be displayed and reloading the page may be necessary Lines to display in graph All 6 lines cells have a checkbox Active cells have a colour assigned to them If a cell is not active the assigned colour is grey When hovering over an active cell the latest opening cell time is displayed Flow rate OLR and HRT The two graphs show variation in time of the gas flow rate OLR and HRT respectively calculated and reported in real time The values displayed in the graphs are normalized to 1 0 atm 0 C and zero moisture content By hovering the mouse pointer over a specific measurement point information regarding this measurement can be obtained e g Line 6 Time 2014 04 05 18 00 Flow rate 173 33 Nl day OLR 3 00 g VS I day HRT 33 33 days 45 bIOprocess ON Lines to display in graph Gas production evaluation Y y y Y Y y SGP NLL day 1 223 1 118 1 498 1 437 1 238 1 475 E q ee y a Duration days 22 95 22 95 2295 15 77 2295 2295 Reset Reset Reset Reset Reset Reset Fl OW rate NL day Use right mouse button to navigate Select region to zoom in by using left mouse bution Lastweek Zoom out 2014 04 02 2014 04 03 2014 04 03 2014 04 04 2014 04 05 2014 04 05 2014 04 06 2014 04 06 2014 04 07 2014 04 07 2014 04 08 2014 04 09 5 46 06 40 20 33 10 26 00 20 14 13 04 06 18 00 07 53 21 45 11 40 01 33 OLR HRT g L day days 2014 04 02 2014 04 03 2014 04 03 2014 04 04 2014
68. ommunication sent to the Licensor or its representatives including but not limited to communication on electronic mailing lists source code control systems and issue tracking systems that are managed by or on behalf of the Licensor for the purpose of discussing and improving the Work but excluding communication that is conspicuously marked or otherwise designated in writing by the copyright owner as Not a Contribution Contributor shall mean Licensor and any individual or Legal Entity on behalf of whom a Contribution has been received by Licensor and subsequently incorporated within the Work 2 Grant of Copyright License Subject to the terms and conditions of this License each Contributor hereby grants to You a perpetual worldwide non exclusive no charge royalty free irrevocable copyright license to reproduce prepare Derivative Works of publicly display 65 bloprccess ROL publicly perform sublicense and distribute the Work and such Derivative Works in Source or Object form 3 Grant of Patent License Subject to the terms and conditions of this License each Contributor hereby grants to You a perpetual worldwide non exclusive no charge royalty free irrevocable except as stated in this section patent license to make have made use offer to sell sell import and otherwise transfer the Work where such license applies only to those patent claims licensable by such Contributor that are necessarily infringed by th
69. on Unit _ Power source THERMOSTATIC WATER BATH a Start up the thermostatic water bath using the main switch b Set the operating temperature GAS VOLUME MEASURING DEVICE a If an anaerobic condition needs to be established during the experimental setup the reactor headspace shall be flushed with Nz or a mixture of N2 CO gt gases Before flushing the system with gas to create anaerobic conditions disconnect the Tygon tube from the connection block on the Unit BCR B B4 Failing to do so might damage the equipment b Connect the gas source to the Tygon tubing with the red tube clamp c Open the red tube clamp d Flush the system with a low gas flow 1 e 2 5 to 10 I min gently for 60 120 seconds e Stop the flush gas and close the red tube clamp f Disconnect the flush gas source g Re connect the Tygon tubing to the Unit BCR B B4 25 DIOPrScess ONTROL Repeat the procedure for all the reactors used in the test Important Before flushing the system disconnect the Tygon tube from Unit BRS B in order to eliminate the risk of damaging built in check valves in Unit BRS B by the high pressure gas flow After flushing the system and before starting the data logging manually open all the flow cells in the gas measuring device in order to release any remaining flush gas trapped in the flow cell chambers SOFTWARE a b d Make sure that the shielded Ethernet cord is connected to
70. rators and other industry actors in over 30 countries within the Americas EMEA Africa and Asia Pacific regions In 2012 BPC launched the BioReactor Simulator BRS an analytical device for the control and monitoring of anaerobic fermentation processes in a continuous mode of operation The BRS 1s an ideal instrument and platform for gaining knowledge and experience of the continuous operation of biogas production processes as well as serves as the complementary tool to AMPTS II which is used for feedstock characterisation and optimisation The system is controlled by a web based software running on an efficient cloud computing solution accessible from any computer or mobile device with an internet connection Taking into consideration the high number of the AMPTS units in use today around the world it is expected that BRS will have a similar impact on the biogas industry becoming the technology of choice for universities private labs and biogas operators interested in the laboratory scale simulation of continuously operated full scale biogas digesters bloprccess ROL 2 GENERAL INFORMATION The process of anaerobic degradation is highly complex and dynamic where microbiological bio chemical and physico chemical aspects are closely interrelated For optimisation purposes fermentation tests at laboratory scale are used to determine feedstock characteristics and to simulate continuous operations of biogas reactors In general for broadl
71. rm a simple leakage test for each reactor by creating some overpressure by blocking one of the metal tube ports and injecting air from the remaining metal tube port then immerse the reactor in water to check if there are air bubbles escaping from the reactor Make sure that all tubes are properly connected and there 1s no potential leakage anywhere Add the sample e g 2000 g of inoculum into the reactors Use at least duplicates for statistical significance see Appendix A for more details HH e a ail WN pod F f 4 eN l Y e i A y Lubricate rubber stoppers A2 on the side that is in contact with the glass bottle preferably with silicone spray or silicone stick Place the rubber stopper A2 with two metal tubes and the bent stir rod A8 connected in the opening of each bottle A3 and press the rubber part in order to close the bottle Avoid pressing the metallic rod as this will lead to separation of the white plastic cap from the bottom of the stirring rod and allow gas or liquid leakage from the reactor Then pull out the metallic rod from the bent stirrer into the helical coupling A6 and attached it before placing the plastic screw thread cap motor support A7 Screw until the thread on the bottle is no longer visible and the lid is properly sealed Connect the bent stir rod A8 to the motor by carefully tightening the two screws on the helical coupling Care should be taken so that the helical coupling is not touchin
72. s connected to the gas flow measuring device both the inlet and outlet ports should be initially closed In order to avoid any perturbation of the system during the feeding step the Tygon tubing from the reactor to the gas flow measuring unit should be closed using a tubing clip e g 25 mm First open the outlet port After connecting a syringe filled feedstock to the silicone tubing from the inlet port open the inlet port and add a certain amount of substrate to the reactor The pressure created in the reactor during the addition of substrate will normally be high enough to discharge an equal amount of sludge At the end of the process the inlet and outlet ports are to be closed the tubing clip disconnected from the Tygon tubing and the system will continue to register the accumulated volume of gas The procedure can be repeated several times per day depending on user needs PROCESS OPERATION IN CONTINUOUS MODE The design and operation of a biogas plant is based on a combination of economic and technical considerations Obtaining the maximum biogas yield by complete digestion of a substrate would require a long retention time inside the digester and a correspondingly large digester size In practice the choice of system design digester size and type or of applicable retention time is always based on a compromise between achieving the highest possible biogas yield and having a 56 DIOPrScess ONTROL justifiable plant economy Proc
73. s not enough The Motor Controller is the hub of the agitation system It interprets the speed signal sent from the Unit C of the AMPTS II and controls the direction of the motors All the mixers receive the same information from the Motor Controller The picture below shows the control panel on the front side of the Motor Controller ON OFF System ON OFF Motor cable Timer control LED switch connector Signal control top Auto ON OFF Power inlet Ground bottom motor directions 14 DIOPrScess ONTROL The ON OFF switch shown in the picture above turns the power of the Motor Controller on and off When the switch is ON in the upper position the red LED will be lit up to indicate that the system is active It is recommended to set the switch to OFF before connecting disconnecting the Motor Controller to from the power mains When the AUTO switch on Motor Controller is set to OFF the mixers will be operated in continuous rotation mode i e the mixers will always rotate in the same direction Setting the switch to ON will activate the AUTO reversing mode which will make the Motor Controller change the mixer directions at regular intervals For the Auto reversing mode to work as intended the DIRECTION switch on each motor needs to be in the AUTO middle position as well If the switch is in either CW or CCW position the mixer will start and stop but never change direction See section Direction Switch for further explana
74. s or losses even 1f such Contributor has been advised of the possibility of such damages 9 Accepting Warranty or Additional Liability While redistributing the Work or Derivative Works thereof You may choose to offer and charge a fee for acceptance of support warranty indemnity or other liability obligations and or rights consistent with this License However in accepting such obligations You may act only on Your own behalf and on Your sole responsibility not on behalf of any other Contributor and only if You agree to indemnify defend and hold each Contributor harmless for any liability incurred by or claims asserted against such Contributor by reason of your accepting any such warranty or additional liability END OF TERMS AND CONDITIONS APPENDIX How to apply the Apache License to your work To apply the Apache License to your work attach the following boilerplate notice with the fields enclosed by brackets replaced with your own identifying information Don t include the brackets The text should be enclosed in the appropriate comment syntax for the file format We also recommend that a file or class name and description of purpose be included on the same printed page as the copyright notice for easier identification within third party archives Copyright yyyy name of copyright owner Licensed under the Apache License Version 2 0 the License you may not use this file except in compliance with the License Yo
75. t least one of the following places within a NOTICE text file distributed as part of the Derivative Works within the Source form or documentation if provided along with the Derivative Works or within a display generated by the Derivative Works if and wherever such third party notices normally appear The contents of the NOTICE file are for informational purposes only and do not modify the License You may add Your own attribution notices within Derivative Works that You distribute alongside or as an addendum to the NOTICE text from the Work provided that such additional attribution notices cannot be construed as modifying the License You may add Your own copyright statement to Your modifications and may provide additional or different license terms and conditions for use reproduction or distribution of Your modifications or for any such Derivative Works as a whole provided Your use reproduction and distribution of the Work otherwise complies with the conditions stated in this License 5 Submission of Contributions Unless You explicitly state otherwise any Contribution intentionally submitted for inclusion in the Work by You to the Licensor shall be under the terms and conditions of this License without any additional terms or conditions Notwithstanding the above nothing herein shall supersede or modify the terms of any separate license agreement you may have executed with Licensor regarding such Contributions 66 DIOPrScess ONTRO
76. t the anaerobic bacteria microorganisms remain in the system High methane yields can often be achieved through long SRTs and MRTs whereas high methane production rates require high OLRs and short HRTs Long SRTs can be attained by reducing the loading rate or by retaining the solids or removing the liquid The latter procedure permits both long SRTs and short HRTs In case of a digester using a CSTR configuration its SRT and MRT are equal to the HRT Too short an HRT can lead to a washing out of the bacteria due to the fact that more bacteria is leaving the digester than can be reproduced which can cause digester crashes As a recommendation the HRT should be kept above 20 days for CSTRs to make sure there is no risk of bacteria cell washout A high HRT will also lead to a longer time for the bacteria to degrade the substrate which in turn will increase the gas yield However this will also lower productivity in most cases Therefore it is important to find a good balance for HRT 57 DIOPrScess ONTROL The HRT days can easily be calculated by dividing the active volume of a digester V L with the average inflow Fin l day according to the following equation HRT E in THE RECOMMENDED VALUES FOR THE PROCESS OPERATION PARAMETERS FOR CSTRS ARE PRESENTED IN THE FOLLOWING TABLE Recommended value Comment OLR gt 3 g VS Ixday Varies from process to process changes in OLR should be conservative HRT 30 days S
77. t will be used If NTP setting the time and date of the system automatically 1s to be used make sure that check box named Syncronize time automatically over the internet is marked Otherwise make sure that the check box is not marked make sure that the time and date on the computer being used to setup the BRS is set properly and click on the button marked Use this time for the instrument to set the internal clock of the BRS to the same time and date as that of the computer Enter the required network information followed by selecting Apply network settings and clicking Yes in the confirmation dialogue Wait approximately 5 minutes for the BRS to change the settings and reboot Disconnect the power supply from the wall power socket Disconnect the provided shielded Ethernet cable from the computer not the Unit BRS B and connect it to the internal network or other suitable network equipment Connect the power supply to a wall power socket 35 bloprccess ONTROL 9 3 FUNCTION AND OPERATION LOG IN DIOPrOcess ON ER ch Welcome to Bioprocess BRS web application Email Password Remember me F Sign in The page 1s used to log in to the BRS a Enter the following website address https brs bioprocesscontrol com b Enter your assigned e mail address c Enter your password bioprocesscontrol default password Please note that this password should be changed by the user for security purposes
78. te amount g c Substrate organic amount gVS d Substrate concentration e Substrate type 4 In the Discharge Data sheet a Time hours days since the start of the experiment b Sludge amount g 28 bloprccess ROL The generated file contains the volume of gas already normalised 1 0 standard atmospheric pressure 0 C and zero moisture content The calculations are carried out to compensate for pressure temperature and saturated moisture content at the above given conditions based on the values of pressure and temperature registered by the sensors from Unit BRS B 29 DIOPrScess ONTROL 9 BRS SOFTWARE 9 1 USERNAMES AND PASSWORDS The BRS provides user interaction through two different interfaces the cloud web interface and the local machine web interface The cloud web interface is used to control the system and experiments during normal operations while the local machine interface is used for network configuration time configuration and upgrading Before using the system it is important to make note of the default passwords and for safety reasons change them to user defined ones Always take care to store the passwords in a safe location for later reference If the passwords are forgotten or misplaced the cloud interface password can be reset by BPC upon proven ownership of the machine and identity while the local machine interface can be reset by utilising the reset function located on the back of the unit
79. te down the initial settings e g IP address subnet mask c Select Manually from the configure IPv4 dropdown menu and enter the following values in the fields 1 3 IP Address 192 168 10 10 1 4 Subnet mask 255 255 255 0 d Select Apply ALL SYSTEMS To access the instrument open a web browser Google Chrome is recommended as it provides the best and most compatible feature set for the BRS In the address field enter http 192 168 10 11 Bellow is located a quick guide to make sure that all settings have been made correctly 34 DIOPrScess ONTROL BRS IP Address 192 168 10 11 Subnet mask 255 255 255 0 Computer IP Address 192 168 10 10 Subnet mask 255 255 255 0 Please note that the IP address for the computer and the IP address for the BRS are different This 1s a design requirement of the IP protocol Care needs to be taken so that the same address is not used in both locations as it will render the system inaccessible from the designated computer After setting up the computer so it 1s able to communicate with the BRS system please follow the steps below a b c d e g h Log in locally to the BRS by clicking on login to the BRS system and entering the user name user and the password bpc In the web browser window showing the BRS local web application click on the tab marked System On the right hand side select the appropriate time zone where the instrumen
80. temperature a disconnected motor cable or power loss If this happens the mixer can be reset with a quick press on the RESET button The RESET button is marked on the very top of the motor unit just above the actual button It can be reached by using a pen or other pointy object If the mixer was reset successfully the red LED will turn off POWER The Motor Controller is powered by a 24 V DC 2 71 A power supply adapter The power supply is then distributed serially from the Motor Controller through the 8 pin signal cables to each motor unit WARNING The power supply always has to be disconnected from the Motor Controller before removing or connecting any of the cables from the motors 19 DIOPrScess ONTROL 7 4 TECHNICAL CHARACTERISTICS GENERAL Usage indoor Power supply 100 or 240 V 50 60 Hz thermostatic water bath and power adapter Tubing material Tygon ID 3 2 mm OD 4 8 mm Weight approx 25 kg SAMPLE INCUBATION UNIT Maximum number of reactors per system 6 Reactor material glass standard or stainless steel Reactor volume 2 standard 5 and 10 Power consumption 1300 W maximum Dimension 53x33x24 cm Temperature control up to 95 C precision 0 2 C Mixing in the reactor mechanical adjustable interval and speed maximum speed 140 rpm GAS VOLUME MEASURING DEVICE Working principle liquid displacement amp buoyancy Built in sensors pressure and temperature Data acquisition
81. the gas volume measuring device Unit BRS B and a local internal network or other suitable network equipment Make sure that the network cable is connected prior to connecting the power supply Follow the instructions presented in Chapter 9 BRS Software step by step for accessing the software interface through an Internet browser such as Google Chrome or the latest version of Internet Explorer Using Google Chrome is always recommended When software and experiment configuration have been completed start the data logging function of the BRS software In the Control menu activate all the cells for data registration Open each cell in the gas volume measuring device manually several times and follow the corresponding result of each opening on the plots in the Graph menu of the software to make sure that both the detection system and data acquisition system function properly Check the files obtained when generating a report in the Download report menu Download these files and open them using Microsoft Excel 2007 or a later version Re start the BRS data acquisition by pressing the End experiment and Start experiment buttons for each individual cell 8 3 MONITORING REACTORS a b If possible perform a GC analysis to determine the fraction of methane in the raw biogas samples or simply estimate the methane content based on literature studies or previous experiment results for a similar substrate sample Periodically check t
82. tion of the different combinations of settings on the Motor Controller and the motor units SIGNAL CABLE The Motor Controller receives signals from the test system through an analogue signal cable pictured below WARNING The position of the signal and grounding terminals of the connectors are reversed on the Motor Controller compared to the AMPTS II system On the Motor Controller the ground is at the bottom terminal see the picture below Always make sure the proper cable is used and that it is inserted the correct way or the system could be permanently damaged 15 bioprcocess CONTROL Top connector for signal control 0 12 V Bottom connector for grounding MOTOR CABLES The signals from the Motor Controller are distributed to each motor unit through the motor cables see picture below They should be connected serially and are fastened to the motor units with the help of latches The 1 5 m cable is used only to connect the MOTOR output on the Motor Controller to the first motor is MIXING INTENSITY The motors can be operated at different speeds ranging from 10 200 RPM The speed 1s adjusted linearly between 10 and 200 RPM referred to as per cent 5 100 in the AMPTS II software At a DC signal of 0 zero V the motor 1s out of function and at 12 V it is rotating at top speed 200 RPM An acceleration deceleration ramp is built into the system to provide a smooth transition between different speeds
83. to do so subject to the following conditions e The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software 61 DIOPrScess ONTROL THE SOFTWARE IS PROVIDED AS IS WITHOUT WARRANTY OF ANY KIND EXPRESS OR IMPLIED INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM DAMAGES OR OTHER LIABILITY WHETHER IN AN ACTION OF CONTRACT TORT OR OTHERWISE ARISING FROM OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE jQuery Caret Copyright c 2010 C F Wong http jquery plugin buss hk Permission is hereby granted free of charge to any person obtaining a copy of this software and associated documentation files the Software to deal in the Software without restriction including without limitation the rights to use copy modify merge publish distribute sublicense and or sell copies of the Software and to permit persons to whom the Software 1s furnished to do so subject to the following conditions The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software THE SOFTWARE IS PROVIDED AS IS WITHOUT WARRANTY OF ANY KIND EXPRESS OR IMPLIED INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY FITNESS FOR A P
84. u may obtain a copy of the License at http www apache org licenses LICENSE 2 0 67 DIOPrScess ONTROL Unless required by applicable law or agreed to in writing software distributed under the License is distributed on an AS IS BASIS WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND either express or implied See the License for the specific language governing permissions and limitations under the License Knockout The MIT License Copyright c lt year gt lt copyright holders gt Permission is hereby granted free of charge to any person obtaining a copy of this software and associated documentation files the Software to deal in the Software without restriction including without limitation the rights to use copy modify merge publish distribute sublicense and or sell copies of the Software and to permit persons to whom the Software is furnished to do so subject to the following conditions e The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software e THE SOFTWARE IS PROVIDED AS IS WITHOUT WARRANTY OF ANY KIND EXPRESS OR IMPLIED INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM DAMAGES OR OTHER LIABILITY WHETHER IN AN ACTION OF CONTRACT TORT OR OTHERWISE ARISING FROM OUT OF OR IN CONNECTION WITH THE SOF
85. urrender the rights These restrictions translate to certain responsibilities for you if you distribute copies of the software or if you modify it For example if you distribute copies of such a program whether gratis or for a fee you must give the recipients all the rights that you have You must make sure that they too receive or can get the source code And you must show them these terms so they know their rights We protect your rights with two steps 1 copyright the software and 2 offer you this license which gives you legal permission to copy distribute and or modify the software Also for each author s protection and ours we want to make certain that everyone understands that there is no warranty for this free software If the software is modified by someone else and passed on we want its recipients to know that what they have is not the original so that any problems introduced by others will not reflect on the original authors reputations Finally any free program is threatened constantly by software patents We wish to avoid the danger that redistributors of a free program will individually obtain patent licenses in effect making the program proprietary To prevent this we have made it clear that any patent must be licensed for everyone s free use or not licensed at all The precise terms and conditions for copying distribution and modification follow TERMS AND CONDITIONS FOR COPYING DISTRIBUTION AND MODIFICATION 0
86. w rate normalisation e Real time calculation and visualisation of key parameters such as OLR HRT SGP e Interactive feeding and discharging support e Possibility of running feeding and discharging in manual or automatic modes e Generation of a standardised report with all recorded and interpreted data e Remote monitoring of experiments e High capacity for data logging and handling e One of the most cost competitive solutions on the market today DIOPrScess ONTROL 3 DELIVERY CHECKS Upon delivery unpack and check that the contents match with what is listed below in the Section Box Content If the packaging or equipment 1s broken or damaged at delivery please a Document and take photos of the parts and packaging b Inform the transport company at the time of delivery c Make sure that the transport company documents the incident d Inform the seller of the incident BOX CONTENT See also Chapter 7 Equipment Description Design Function for pictures of the included parts UNIT BRS A 6 glass bottles with 3 ports 2 reactors 6 plastic caps with agitators motors including 5 short motor cables 1 long motor cable from Motor Controller to first motor unit 1 Motor Controller 1 signal cable 6 rubber stoppers with 2 metal tubes 1 plastic tube and rotating shaft for mixing 1 thermostatic water bath 18 1 1 plastic glass lid for the water bath with 6 circular openings for the reactors 6 helical couplings tool 6
87. y designed test programs the combination of batch and continuous methodologies should be used A large number of batch tests running in parallel deliver results on feedstock characteristics whereas a continuous test simulates long process conditions spanning a large time frame 2 1 CONVENTIONAL CONTINUOUS FERMENTATION TEST In a conventional continuous fermentation test the organic matter is added either in stages or continuously to the reactor The end products are periodically or continuously removed resulting in a constant and predictable production of biogas A single digester or multiple digesters in sequence may be used such as continuous stirred tank reactors CSTR upflow anaerobic sludge blankets UASB expanded granular sludge beds EGSB internal circulation IC reactors etc The objective of the fermentation tests in the defined continuous procedure is to obtain reliable long term base data about the gas yield and its composition and to build up a comprehensive picture regarding the degradation of the organic material the course of fermentation and any problems in the degradation process which may occur In fact results from continuous lab scale fermentation tests have on several occasions been shown to provide a good representation of the full scale operation With the help of continuous fermentation tests it should also be possible to determine how the properties of substrates affect the fermentation process and what process

Download Pdf Manuals

Related Search

Related Contents

Samsung PL-42E71S manual do usuário  FLB 3000 A1_LB 4 deck.indd    Delta A00543 Pressure Washer User Manual  User Manual - BATS Exchange  Zotac ZBOXSD-ID13-U barebone  operating instructions  Installations fioul et accessoires de S.A.V.  Armitron 6P00 User's Manual  Jetzt downloaden  

Copyright © All rights reserved. Failed to retrieve file