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Multidrop Combi nL User Manual

1. Thermo Scientific Multidrop Combi nL User Manual Rev 1 1 Cat no NO7171 Copyright Copyright 2012 Thermo Fisher Scientific Inc All rights reserved First edition published in 2007 Reproduction of the accompanying user documentation in whole or in part is prohibited Trademarks FILLit is a trademark and Microtiter Multidrop and Nalgene are registered trademarks of Thermo Fisher Scientific Inc and its subsidiaries Decon is a registered trademark of Decon Laboratories Limited Micro 90 is a registered trademark of International Products Corp Microside SQ is a registered trademark of Global Biotechnologies Inc Teflon and Virkon are registered trademarks of E I du Pont de Nemours and Company or its affiliates All other trademarks and registered trademarks are the property of their respective holders Disclaimer Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information This manual supersedes all previous editions Remarks on screenshots Screenshots may be sligh
2. Plate type on page 37 Dispensing volume and liquid on page 39 Column Row selection on page 40 Save and edit protocol on page 54 Dispensing speed on page 48 Settings Shaking on page 47 Dispensing offsets on page 48 Predispensing volume on page 50 Dispensing direction on page 51 Device information on page 59 Computer interface on page 60 Startup protocol on page 61 Instrument settings on page 62 Calibration on page 63 RSS d Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 35 Routine Operation Dispensing ped Dispensing ma na af it JL Q 36 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific You can immediately start dispensing by following the quick start guidelines below Routine dispensing only requires to key in a few buttons Power ON the instrument Insert the priming vessel and install the reagent reservoir and the reagent Close the protective cover Make sure you are in the Main menu Select the plate type and insert the plate Select the dispensing volume and the liquid Press the PRESSURE button and wait till the pressure has risen Only at startup Keep the PRIME button down until the tubings are completely filled Press the START button to start the protocol The instrument dispenses the plate
3. The keyboard is shown in Figure 4 19 E EMPTY AA Ea PRIME Control buttons START START and STOP control buttons STOP J MULTIDROP COMB nl Figure 4 19 Keyboard of the Multidrop Combi nL The relevant keys and control buttons are described in detail below The arrow keys are used to select modify and change the parameters and to navigate in the display The OK button is used to accept the selection The START button is used to start the dispensing protocol The STOP button is used to stop the protocol at any time The plate and dispensing valve head are returned to the home position While editing values the changes can be discarded by pressing the STOP button 32 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Thermo Fisher Scientific m E gt H ot SHAKE A g a TIP WASH Hi Display icon Routine Operation Control panel The STOP button is also used to stop the shaking action Note In addition the STOP button is used as an Esc Escape Cancel button A The PRIME button is used to fill the tubings of the dispensing valves and predispense Predispenses as long as you press the button The EMPTY button is used to empty the liquid from the tubings and dispensing valves into the liquid reservoir backflush option The EMPTY button is also used for cleaning the dispensing valves and tubes Refer to Chapter 5 Maintenance Empties
4. e Check after each dispensing that the priming vessel is not filled up if the tube drain is not used A lL f Main Make sure you are in the Main menu Select the plate type Jl 4 Select the dispensing protocol from the protocol list Only protocols for the selected plate type are shown abe Press the PRESSURE button and wait till the pressure has risen Only required at startup Keep the PRIME button down until the tubings are completely filled Press the START button to start the protocol Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 53 Routine Operation Running a protocol Save and edit Go to the Main menu Select the Select and Save Protocol row protocol 7h oo abc lt S y 384 low prof 10mm ly 4 gt Move OK Add STOP Escape Enter the name of your protocol Ww q Use the arrow keys to choose the character you want 54 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific v v jis Backspace button h nen B V save Thermo Fisher Scientific Routine Operation Running a protocol Press OK to enter the character The available characters are a to z 0 to 9 hyphen underscore _ and space Note Use maximum 20 characters including the space character Some of the characters are broader than the others whereby some of the names can display less than 20 characters in the protocol li
5. Parameters selected in the Main menu Factor START Dispense OK Accept e Take the first strip column 1 of a 96 well 1x8 strip plate Thermo Scientific Microtiter 96 1x8 Strip Plate Cat no 95029350 or equivalent and use an analytical balance four digits to set the tare weight to zero the difference after weighing the dispensed liquid to obtain the net value However the easiest way is to set the tare weight of the empty strip to zero so that the net mass of the dispensed liquid can be obtained from the balance without any calculations A Note You can also measure the mass of the empty strip and calculate e Place the plate onto the plate carrier of the Multidrop Combi nL e Press START on the keyboard to start dispensing or STOP to escape at the liquid speed and volume set in Main gt Volume and Liquid The liquid is dispensed into the empty strip a default number of times depending on the set volume to obtain a measurable amount of mass The expected volume necessary to be dispensed is shown above the line for example 179200 nl in the screenshot above e Measure the mass of the strip immediately to avoid evaporation Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 65 Routine Operation Instrument options ACCURACY CALCULATION Convert the obtained mass to volume nl and calculate the accuracy as follows ACCURACY 100 Measured volume Set volume Set volume e Measured v
6. 26 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Installation Operational check 6 Press START e The dispensing protocol selected with the user interface is started e The clamp Figure 3 14 tightens the microplate against the lower left corner of the plate carrier If the protocol or predispense is not started follow the instructions on the display or check Chapter 9 Troubleshooting Guide Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 27 Installation Operational check 28 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Chapter 4 Routine Operation Dispensing The dispensing system of the Multidrop Combi nL is based on a pressure vacuum system that the instrument controls valves The liquid to be dispensed into a well is pushed through a microsolenoid valve by means of compressed air The pressure system can also produce vacuum which is used for the Empty function The principle of the pressure system is shown below Figure 4 15 Pressure Vacuum system Manifold l i i l z _ Dispensing valves Figure 4 15 Principle of the pressure vacuum dispensing system The eight microsolenoid dispensing valves are inserted into the dispensing valve head The modular design is shown below Figure 4 16 Groove for hosing Dispensing valve Manifold tube 8 Dispensing valve head Dispensing valve
7. 41 43 46 63 64 offset 35 38 48 49 parameters 37 precision 91 range 68 speed 15 30 35 39 46 47 48 63 64 65 68 91 95 105 system 13 14 29 valve 29 105 valve head 26 29 32 48 50 52 86 97 98 100 105 valve lifting mechanism 25 49 52 86 97 volume 30 35 36 38 39 40 41 42 43 44 46 63 64 66 68 91 99 disposal of 84 89 instrument 89 materials 84 Thermo Scientific Multidrop Combi nL User Manual 107 E editing a protocol 35 37 45 54 58 emergency situations 69 72 emptying 14 29 33 36 37 41 43 53 65 68 73 74 75 77 78 85 88 91 105 environmental requirements 20 error messages 97 F factor calculation 63 64 66 FILLit Software 3 14 15 30 37 38 39 53 63 66 68 91 101 105 G glossary 105 guidelines for use 6 H hazards 4 72 99 100 103 home position 25 32 52 105 how to decontaminate the instrument 84 how to install the Multiskan FC 92 i icons 34 35 info text 34 installation 3 19 20 21 22 23 36 88 92 category 92 installing the pressure vacuum system reservoir 23 installing the priming vessel 22 installing the transport lock 72 86 88 instrument settings 35 62 buzzer 62 intended use 3 14 internal software 3 14 32 37 59 96 97 99 K keyboard and display 14 18 32 91 L liquid 14 29 30 31 39 63 64 65 66 67 68 factor 15 39 63 64 66 6
8. 7 Keep the transport lock there until needed for future relocation or transportation of the instrument Figure 3 7 Transport lock storage Installing the To install the priming vessel priming vessel 1 Insert the blue priming vessel Cat no NO7271 into its slot on the left of the plate carrier by pressing it over a snap lock Figure 3 8 V Figure 3 8 Inserting the priming vessel 2 Insert a priming vessel tube assembly or cap Cat no N05843 into the drain of the priming vessel or a vessel underneath the drain 22 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Installation Installation setups Installing the To install the pressure vacuum system reservoir that is the reagent pressure vacuum reservoir system reservoir 1 Remove the red luer plugs from the left side of the pressure vacuum system box and from the reagent reservoir Figure 3 9 The red luer plugs avoid particles from entering the pressure vacuum system unnecessarily lt i gt ii i Aa V Ig TAN REAA NAUN gt A P B AN aa 4 Figure 3 9 Removing the red luer plugs 2 Connect the luer connector of the hosing to the pressure vacuum system box Figure 3 10 Connect the other end of the hosing through a similar luer connector to the reagent reservoir Figure 3 11 for producing pressure and vacuum Figure 3 10 Connecting the hosing to the pressure vacuum system Thermo Fish
9. excessive moisture or large temperature fluctuations Make sure the working area is flat dry clean and vibration proof and leave additional room for accessories cables and reagent bottles Make sure the ambient air is clean and free of corrosive vapors smoke and dust Make sure the ambient temperature range is between 10 C 50 F and 40 C 104 F Make sure the humidity is so low that condensing does not occur relative humidity is between 10 and 80 Leave sufficient space at least 10 cm on both sides and at the back of the unit to allow adequate air circulation The Multidrop Combi nL does not produce operating noise at a level that would be harmful No sound level measurements are required after installation Place the instrument on a normal laboratory bench The net weight of the entire equipment is approx 9 6 kg 21 2 lbs The instrument operates at voltages of 100 240 Vac and a frequency range of 50 60 Hz Precautions and limitations 20 Thermo Scientific Multidrop Combi nL User Manual Always ensure that the local supply voltage in the laboratory conforms to that specified on the rating label on the back of the instrument Figure 2 4 Do not smoke eat or drink while using the Multidrop Combi nL Wash your hands thoroughly after handling test fluids Observe normal laboratory procedures for handling potentially dangerous samples Wear proper protection clothing such as disposable glo
10. A Instrument options Device information Thermo Fisher Scientific Routine Operation Instrument options Caution Before deleting always check that you have selected the item you wish to delete A This section describes the instrument parameters All these parameters are set in the Options menu The values shown in the Options menu remain in the instrument memory and are instrument specific not protocol specific Note You do not normally have to change the instrument options in routine use A The device information shows the name of the instrument the internal software version and the serial number of the instrument unit Go to the Options menu Device information R 232 Default protocol 384 lo Instrument settings Liquid factor Senge Options OK Exit Thermo Scientific Multidrop Combi nL User Manual 59 Routine Operation Instrument options Computer interface There are two types of computer interfaces available RS 232 and USB Go to the Options menu Select the Computer interface row Default protocol 384 lo Instrument settings Liquid factor This selection also has to be done when you use the instrument through FILLit Software Refer to Chapter 2 Installing FILLit Software in the FILLit Software for Multidrop Combi nL User Manual Select OK Accept Cat no N07173 Use the Up and Down arrow keys to select the computer interface in use Press OK 60 Thermo Scientific
11. Combi nL User Manual Thermo Fisher Scientific Thermo Fisher Scientific gt D Maintenance Decontamination procedure It is strongly recommended to perform the complete decontamination procedure before relocating the instrument from one laboratory to another or before sending it to service Example of decontaminants e Ethanol 70 e Virkon solution 1 3 e Glutaraldehyde solution 4 e Chloramine T e Microcide SQ 1 64 e Decon 90 min 4 Caution If local or laboratory regulations prescribe regular decontamination it is not advisable to use formaldehyde since even small traces of formaldehyde negatively affect the enzyme being used in EIA tests resulting in bad test results A Warning The decontamination procedure should be performed by authorized trained personnel wearing disposable gloves protective glasses and clothing in a well ventilated room A 1 Prepare the decontaminant for example 1 3 Virkon solution or 200 ml 4 glutaraldehyde solution or another agent recommended by your safety officer 2 Empty the reagent reservoir Ensure that you are wearing disposable gloves 3 Switch off the power Figure 2 4 and disconnect the mains supply cable 4 Disinfect the outside of the instrument using a cloth dampened with 70 ethanol 5 Place the instrument in a large plastic bag 6 Place a cloth soaked in the prepared solution into the bag Ensure that the cloth does not come into contact with the in
12. OK button for 0 5 seconds The Volume value box is now activated bright red Adjust the dispensing volume of the column where the cursor is located using the Up and Down arrow keys Main Volume OK Accept STOP Escape Press OK after selecting the desired volume Use the Down arrow key and select Accept and press OK after selecting the column volumes you wish You can press the STOP button to exit the volume selection without making any changes 42 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Row selection Ay lt gt Setting the row volume Thermo Fisher Scientific Routine Operation Dispensing parameters Row ID EMPTY Clr all 4p Move OK Fill Clear Use the arrow keys to move from one row to the next Press the OK button every time to select a row The selected row changes from red filled to uncolored empty Use the OK button to toggle between a filled or empty row Use the Down arrow key and select Accept and press OK after selecting all the rows you wish OK Accept STOP Escape You can press the STOP button to exit the row selection without making any changes The dispensing volume selected in the Volume and Liquid window see Dispensing volume and liquid on page 39 is shown in the bottom left box You can adjust the dispensing volume for each individual row Thermo Scientific Multidrop Combi nL User Manual 43 Routine Operation Dispensing para
13. Press the EMPTY button to empty the dispensing tubing Power OFF the instrument The pressure will be released Using FILLit PC software Using internal software Dispensing parameters A Plate type Routine Operation The operation of the Multidrop Combi nL dispenser can also be controlled by an external computer and run on FILLit Software for Multidrop Combi nL In addition to the Multidrop Combi nL internal software features you can use multistep protocols that is protocols with several steps e g Dispense Shake Empty etc and create new calibrations for new liquids The user can also download to the instrument protocols with one dispense step and an additional shake step or transfer protocols from one instrument to another Calibrations can also be transferred to the internal software Use FILLit Software to enter the valve correction factors while changing the spare valve For more information refer to the FILLit Software for Multidrop Combi nL User Manual Cat no N07173 This section describes procedures related to the Multidrop Combi nL internal software This section describes the relevant dispensing parameters required to create and edit a protocol All these parameters are set in the Main menu Note Make the plate type selection first because all the other parameters are dependent on the plate type A Caution Make sure the plate lid if used is removed before dispensing A Go to the Main men
14. Select OK Accept gt Save AV Select the protocol using the Up and Down arrow keys Press OK to accept the selection The protocol is now selected Note In the protocol list only the protocols made for the plate type in question are shown A Delete protocol To delete an existing protocol for a particular plate type first select the p gP p plate typ plate type see Plate type on page 37 and then select the Select and Save Protocol row Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 57 Routine Operation Running a protocol Main Settings Options 384 low prof 10mm Abe vu sf cy Default protocol abc 5Select OK Accept gt Save AV Select the protocol using the Up and Down arrow keys gt Press the Right arrow key to enter the edit mode Main Peetmgs Options Protocol name T i v BEDERSH NUKEM lt MGESEEROMRANe GHEE abe Delete button 4 Move OK Add STOP Escape To delete the protocol use the Delete button Use the Down arrow y key and if needed the Left and Right arrow keys to select the Delete button and then the OK button to delete the protocol You cannot delete Delete default protocols the Delete button is not activated Also if you have selected a protocol to be a startup protocol it cannot be deleted The protocol is now deleted 58 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific
15. Thermo Scientific Multidrop Combi nL User Manual 81 Maintenance Regular and preventive maintenance 1 Detach the flexible tube ends from the eight dispensing valves Figure 5 25 2 Detach the hosing from the reagent reservoir and discard the tubing set and manifold 3 Wash the reagent reservoir For more information refer to How to clean the reagent reservoir on page 82 Replace the reagent filter 4 Reconnect the new hosing to the reagent reservoir and the manifold of the new tubing set and place the dispensing valve tube ends into a waste container for example a decanter 5 Prime the tubing with distilled and filtered laboratory grade water 6 Reconnect the flexible dispensing valve tube ends to the dispensing valves Figure 5 26 and press the hosing into the groove Figure 5 31 Figure 5 31 Replacing the tubing set and manifold 7 Prime the tubing to ensure the proper functioning of the dispensing valves in conjunction with the tubing set replacement A Note The manifold is not changed separately but always simultaneously How to clean the Clean the reagent reservoir Figure 2 2 and Figure 4 17 regularly after reagent reservoir and when you change the reagent To clean the reagent reservoir 1 Detach the reagent reservoir 82 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Maintenance Regular and preventive maintenance 2 Rinse or wash the reagent
16. a fixed valve open time The approximate dispensing volumes change according to the liquid properties and dispensing speeds Refer to the example with water Speed 3 in Table 4 1 Table 4 1 Calibration point vs dispensing volume and dispensing times Calibration point Dispensing volume Number of dispensings 1 60 nl 300 2 100 nl 150 3 350 nl 45 4 3 5 yl 18 5 70 ul 2 The dispensed liquids may have different properties which affect the valve open time needed to achieve the required volume Thus several different calibrations are needed to achieve accurate dispensing results For more information refer to the FILLit Software for Multidrop Combi nL User Manual Cat no N07173 30 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Reagent reservoir Pressure vacuum system Thermo Fisher Scientific A Routine Operation Reagent reservoir There are different possibilities for holding the reagent to be dispensed The liquid to be dispensed can be kept directly in the reagent reservoir of the pressure vacuum system Figure 4 17 There is a white reagent filter of 43 um at the bottom of the 250 ml reagent reservoir that filters the solution to be dispensed Nevertheless all reagents still have to be filtered before use Reagent reservoir Figure 4 17 Reagent reservoir connected A 1000 ml reagent reservoir Cat no N07490 with a longer tubing is also available for larger volumes often u
17. as long as you press the button The SHAKE button is used to shake the plate linearly The shaking is started by pressing the SHAKE button and stopped by pressing the STOP button The PRESSURE button is used to raise the pressure at startup Once the pressure has been reached pressing the PRESSURE button again releases the pressure Therefore you can toggle with the PRESSURE button However raising the pressure is started by pressing the PRESSURE button and stopped only by pressing the STOP button if the pressure has not yet been reached Note Pushing in the protective cover releases the pressure A The TIP WASH button is used to remove persistent air bubbles from the hosing microsolenoid valves and to open clogged dispensing valves The tip washing is started by pressing the TIP WASH button The liquid comes in pulses and one channel at a time to maintain a maximum flow through the dispensing valve Tip washes as long as you press the button The main view in the display is shown in Figure 4 20 pm Menu Sy 96 standard 15mm Main view row Full plate abc Default protocol OK Plate type Info text bar Figure 4 20 Main view on the display of the Multidrop Combi nL Thermo Scientific Multidrop Combi nL User Manual 33 Routine Operation Control panel There are three menus in the Multidrop Combi nL user interface Main Settings and Options In routine use you mainly navigate in the Main menu In advan
18. eNe k aeo aE E E S 64 PAC COL CACUNAT ON eae init a aruabeuue dice Aube ac aaseatieaete 66 SELES I SAVEE a E E RTE AO ETG 68 Sbutdo Wii argerian E N OT a 68 Emergency situatio eean i eee eee ies 69 Chapter 5 Ma imtenant esiosa a ite eta 71 Mame nance checklist oreraa erence ren Rh tee mtre ar perme 71 Regular and preventive maintenance ssssssesserssssesssesssressresseeesere T Howto clean thetubines Seia E tae 73 Death y Tita CWA Ce cera one ane a N E A 73 Removing foreign particles ooien eii i e a edie T Disinfeetitig the liguid Pati srresne a es 76 Routine maintenance of the dispensing valves tips eeseeeee 76 Daly AAU CNA CS xaerdettadea teeta tietratmatyek tanbuieande we tanhemteve in 76 TIDAS DINE ainia EEE teapiceeionedecnste IT Ep Ee inee at cre one ncee hase eta tes Ti Periodic TA MEA CS 5c 4 inst rsrer eter vee AE II 8 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Thermo Fisher Scientific Chapter 6 Chapter 7 Chapter 8 Chapter 9 Appendix A Contents How to clean a leaking dispensing valve 0 eeeeeeseeeeeneeeeeeees 77 How to clean a clogged dispensing valve eeeeeeeseeeeeseeeeeeees 79 Replacing a dispensing Valve vscsesssceadsasustetarutucadebuettpeanpencntdewusiests 79 Replacing a tubing set and a manifold eee eeeseeeeeeeeeeeeees 81 How toccleam the reagent reservoir sarien a 82 Howto cean the plate Carrie i aa eanegeieetsca tote aE 83 How toclean the pri
19. flexibility for a wide range of applications Multidrop Combi nL can be used for example in cell and bead based assays and to a greater extent in drug discovery screening processes for dispensing protein solutions BSA detergents dyes and different kinds of solutions Compounds of interest are often solubilized in DMSO that is ready calibrated and in store in the instrument The viscosity of the solutions varies considerably Viscosity describes a fluid s internal resistance to flow and can be thought of as a measure of fluid friction Thus water is thin having a lower viscosity while e glycerol is thick having a higher viscosity The instrument can calibrate the valve open times and dispense with great accuracy liquids with different viscosities Enzyme or antibody solutions which often contain glycerol can be reliably dispensed with the Multidrop Combi nL 14 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Calibrations Advantages of using Multidrop Thermo Fisher Scientific Combi nL Introduction to the Multidrop Combi nL Advantages of using Multidrop Combi nL The standalone instrument has five calibrated liquids in store and is immediately ready for use The liquid factor feature can be used to calibrate set volume points in instrument standalone use The instrument can easily calibrate a new liquid with the Multidrop Combi nL PC software Calibrations are created c
20. has to be installed Once the reagent reservoir is connected the instrument is ready for use Thermo Scientific Multidrop Combi nL User Manual 13 Introduction to the Multidrop Combi nL Intended use Intended use The Multidrop Combi nL is intended for professional research use by trained personnel It is intended for automated nanoliter to microliter volume dispensing of reagent into a variety of microplates and strips in 96 384 or 1536 well plate format Use for self testing is excluded Principle Of The Multidrop Combi nL Figure 1 1 can be operation 1 Used as a standalone instrument with internal software using the keyboard and display 2 Controlled more flexibly with a PC with FILLit Software via an RS 232 or a USB serial port or 3 Used as part of an automation system Pressure vacuum The Multidrop Combi nL has a pressure vacuum based dispensing system system that the instrument controls The liquid is pushed through a microsolenoid dispensing valve by means of compressed air into a well The reagent can be dispensed into rows columns or wells The pressure system can also produce vacuum which is used for the backflush of reagents Empty function Dispensing Reliable low volume dispensing can be carried out with the Multidrop applications Combi nL With a volume range of 50 nl to 50 ul for 96 384 and 1536 well plates with dispensing heights from 5 to 55 mm the Multidrop Combi nL offers outstanding
21. in Figure 2 3 Display OK Plate type EMPTY TIP WASH 5 PRESSURE PRIME STA RT STOP j Keyboard Figure 2 3 Close up of the Multidrop Combi nL keyboard and display Back view The back view of the Multidrop Combi nL instrument is shown in Figure 2 4 Instrument label Pressure vacuum luer connector Serial RS 232C connector USB 1 1 2 0 compatible Pressure vacuum system Figure 2 4 Multidrop Combi nL back view 18 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Delivery check Checking delivery Thermo Fisher Scientific Unpacking Chapter 3 Installation This section covers the relevant procedures to be carried out on receipt of the instrument Check the enclosed packing list against order In case of any deviations contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy Visually inspect the transport package the instrument and the accessories for any possible transport damage If the carton has been damaged in transit it is particularly important that you retain it for inspection by the carrier in case there has also been damage to the instrument Neither the manufacturer nor its agents can be held responsible for any damage incurred in transit but the manufacturer will make every effort to help obtain restitution from the carrier Upon receipt of the carrier s inspection report arrangements will be made for repa
22. option available Thermo Scientific Multidrop Combi nL User Manual 91 Technical Specifications Safety specifications Safety specifications Live parts A In conformity with the requirements 92 Thermo Scientific Multidrop Combi nL User Manual This section describes the safety specifications for the Multidrop Combi nL instrument The instrument is safe to operate with the covers fitted and they must not be removed during operation The covers protect the user from live parts and they should only be removed after switching the instrument off and disconnecting the mains supply cable and only by suitably qualified maintenance and repair personnel Warning The instrument uses voltages dangerous for human beings Before removing any covers disconnect the instrument from the power supply Multidrop Combi nL bears the following markings Type 838 100 240 Vac 50 60 Hz 100 VA GE cCSAus monogram Multidrop Combi nL conforms to the following requirements 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive EMC FCC Part 15 Subpart B Class B July 2004 2002 96 EC WEEE Directive 2006 42 EC Machinery Directive The safety specifications are also met under the following environmental conditions in addition to or in excess of those stated in the operating conditions Altitude Up to 2000 m Temperature 5 C to 40 C Humidity Maximum relative humidity 80 for temperatures u
23. tee 37 Dispensing PAT AMICLClS cepassnsaroedsbantsasshient inamnenneaseeneatenetoonuatetbatuieats r Plate yp Earne E acerca oe 37 Dispensing volume and Guid occ saacitieee racemes 39 Columns Row SeleCu Oleg cinots canvass ENE 40 FONDATE nE ages alae 40 CO im SELECT 1 aes arsed oped E EAE 4 Seting the colunn volume ty iott eaten ide wale aeetie tee oaade 4 TRONS Sl CUO Ni 5 ces Saceawes eens E teats 43 Setting the TOW VOUE x oieinadnisistousttmssacinn tei ie teieeeieeienies 43 Selecting columns and rows Selected wells eee eeseeeeeeees 44 PROTOCOL Seb 1S sirena i A E E E 45 IDS Penis NOS PCC ieee ecg a E T ceases eas eptceneese 46 DS EN A lee adore ccleaner A eer E N 47 Dispennet bilo mmernren Trent rem tener ee eenrrr crn Te cen eee terre en rere te 48 FM pal ware T Sysco es A I E rn eee 49 DO OMS cate tiethes Baten sah ace eat Sect eemecet imate tnoseh de teectea cutee Gytane 50 P redispen sine VON IMG yates cpsecey cadences ea aetna eter ateaadet 50 Dispens ine GITSC ti O Nosia O E 51 Runne a LOCOCO lesane 53 pave and edit prototo penne artnet en a N 54 SElECE PLOLOCOl oprta E E E T EA 57 Delete protocol cyni iaar R 57 Postument opoNi E E a 59 Device norna o ea E T E neem 59 COmipUrer interia E ea A A 60 Startup PLOLOCO epr E E ER naa 61 Dastamen tset NGS ascarid ntarctemimena tare ayins ex ntearteas 62 OANA Cg ares es secede ETA 63 Liquid factor standalone calibration eceeeeseeeeeseeeeeeeeeeees 63 PSC CUEACY
24. the dispensing height and or the dispensing offset Press OK Height The default dispensing height depends on the plate type see Table 4 3 You can change the dispensing height values if the plate used is not the standard one specified in the plate type list an Settings oo Height OK Accept Use the Up and Down arrow keys to set the dispensing height While you change the offset value the dispensing valve lifting mechanism moves accordingly The height is set in 0 05 mm increments in both the up and down directions The minimum height value depends on the selected plate type see Table 4 3 The default dispensing height is 1 mm above the selected plate Note You can adjust the dispensing height of low profile plates below the plate height A Press OK If the OK button is not pressed within 60 seconds the adjustments are disregarded and the main view returns to the Settings menu z axis the dispensing valve lifting mechanism moves Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 49 Routine Operation Protocol settings X Y offset qdPAayv Predispensing volume The default dispensing X Y offset is 0 0 0 0 mm You can change the x and y axis offset values if the plate used is not the standard one specified in the plate type list see Table 4 3 j T Settings opions 4qp4 X 7 offset OK Accept Use the Left and Right arrow keys to set the dispensing X Y offs
25. you believe that liquid has entered the Multidrop Combi nL first switch the instrument off Figure 2 4 and unplug the instrument Carry out corrective measures Refer to Emergency situations on page 69 and Decontamination procedure on page 84 for aid If necessary contact your local Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department Refer to cC gt gt Packing for service on page 87 Thermo Fisher Scientific A A How to clean the tubings Daily maintenance Removing foreign particles Thermo Fisher Scientific Maintenance Regular and preventive maintenance Warning There are no user serviceable components inside the instrument The operator should not remove the instrument cover A Warning If any surfaces are contaminated with biohazardous material a mild sterilizing solution should be used A Caution Do not autoclave any part of this instrument except the priming vessel reagent reservoir and the tubing set and the manifold A This section is divided into daily maintenance and maintenance procedures when foreign particles have to be removed from the tubings The basic maintenance procedure should be performed regularly and on a daily basis to ensure proper tubing operation 1 Filter all reagents detergents solutions and water before use to remove solid particles 2 Rinse the tubings with distilled and filtered laboratory grade water al
26. 7 68 105 factor calculation 63 64 66 luer connector 23 108 Thermo Scientific Multidrop Combi nL User Manual maintenance 3 6 31 33 69 71 72 73 76 77 88 92 manifold 24 29 72 73 77 81 82 88 95 101 105 transport plug assembly 24 88 markings 105 N naming a protocol 35 45 54 nanodispensing 31 navigating 32 34 0 opening an existing protocol 57 operation principle 14 operational check 25 ordering information 101 P packing instructions 19 88 packing list 19 plate 13 14 91 carrier 22 25 27 50 52 65 71 83 86 88 97 105 type 35 36 37 38 39 40 45 49 50 51 52 53 57 61 62 91 precautions and limitations 20 predispense 27 33 50 105 predispensing 35 50 51 97 volume 35 50 51 pressure 13 14 23 26 29 30 31 33 36 46 47 53 63 68 69 75 77 79 83 84 88 91 95 97 101 105 pressure vacuum system 13 14 23 29 31 88 101 prime 26 we 33 35 36 50 5 53 68 Ts 76 78 81 82 91 97 98 105 priming vessel 22 26 36 53 71 73 83 84 86 88 91 96 97 98 101 105 priming volume 35 50 51 principle of operation 14 protective cover 26 33 36 69 75 79 97 98 105 protocol 27 32 36 44 45 53 54 55 56 57 58 59 61 62 67 105 deleting 57 97 editing 35 45 54 naming 35 45 54 opening 57 running 53 saving 35 45 54 55 Thermo Fisher Scientific settings 45 p
27. 8 Tubing set and manifold hosing from reagent reservoir to f hosing from reagent reservoir to ma manifold 8 dispensing valve tubes Figure 4 16 Eight dispensing valves inserted into the manifold Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 29 Routine Operation Dispensing valves The dispensing valves are suitable for high precision and high speed dispensing of liquids The valve nozzle bore geometry is designed to achieve an optimum droplet forming when the dispensed liquid volume is discharged from the valve The wetted components are made from chemically resistant materials The wetting materials of the microsolenoid dispensing valves Cat no N07494 consist of stainless steel polyetheretherketone PEEK sapphire and ruby The functional principle of the valve is Not activated Closed and Activated Open Depending on the loading pressure at the reagent reservoir the duration of the opening pulse determines the dispensing volume Calibration A calibration defines the valve open times for different volumes at a certain air pressure Calibrations are created calculated and transferred with the Multidrop Combi nL PC software Calibration is a combination of a liquid to be dispensed and a speed Calibration will ensure accurate dispensing with the instrument A calibration contains a series of five calibration points of which each consists of a volume time pair Each calibration point has
28. For PC software related issues refer to the Thermo Scientific FILLit Software for Multidrop Combi nL User Manual Cat no N07173 For the latest information on products and services visit our websites at http www thermoscientific com http www thermoscientific com multidrop In our efforts to provide useful and appropriate documentation we would appreciate any comments you may have on this user manual to your local Thermo Fisher Scientific representative Thermo Scientific Multidrop Combi nL User Manual 3 About This User Manual safety symbols and markings Safety symbols These symbols are intended to draw your attention to particularly important information and alert you to the presence of hazards as and markings F i indicated Safety symbols and The following symbols and markings appear on the type label and the P gs app yp markings used on the instrument itself Multidrop Combi nL Power ON a Power OFF a Serial number a Catalog number a Date of manufacture a Consult instructions for use a WEEE symbol This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC A Bc Ho 4 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Warning and other markings used in the documentation Thermo Fisher Scientific DEBS About This User Manual safety symbols and markings The following symbols and
29. Multidrop Combi nL User Manual Thermo Fisher Scientific Routine Operation Instrument options Startup protocol You can set which protocol is automatically selected in the Main menu when the Multidrop Combi nL is powered on Go to the Options menu Select the Startup protocol row Main f Settings d Options Device information RS 232 Instrument settings Liquid factor Default protocol 384 low prof 10mm Abc Abed Select OK Accept Use the Up and Down arrow keys to select the startup protocol The protocols are listed grouped and indented under each plate type Press OK The protocol name is appended with the plate type DEn Options Device information RS 232 Instrument settings Liquid factor OK Startup protocol Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 61 Routine Operation Instrument options When the instrument is started up the next time the plate type and the protocol are those just selected Note that the sandglass window always appears while the Save action is in progress in the Options menu foo ff eee lt select OK Accept Instrument settings You can choose whether or not the instrument produces a sound for different functions Note that all functions make a certain sound Go to the Options menu Select the Instrument settings Buzzer row n e Options Device information R S 232 Default protocol 384 lo Liquid facto
30. These additional parameters are set in the Settings menu The values shown in the Settings menu are those set for the plate type that is selected in the Main menu Note The protocol settings do not normally have to be changed in routine use A Note An exclamation mark will appear in the menu tab and in the circle on the main view row in question when the setting differs from the default setting A TRA Shaking Offsets Predispense 100nl Direction OK Shaking The changes made to the protocol settings only remain in the instrument memory if the protocol is saved with a name see Save and edit protocol on page 54 If the protocol is not saved the changes only remain effective until the plate type is changed or the instrument is switched off Thermo Scientific Multidrop Combi nL User Manual 45 Routine Operation Protocol settings Dispensing speed The default dispensing speed that is the pressure is automatically set according to the liquid used Refer to the Volume and Liquid window shown in Dispensing volume and liquid on page 39 However you can change the dispensing speed if the liquid has been calibrated for several speeds Go to the Settings menu Options O Shaking OFF must Offsets m j Predispense 100nl Settings opio 5peed OK Accept E g 1 BSA E g Water Use the Up and Down arrow keys to select the speed The available and calibrated speeds a
31. according to the density of the liquid The Liquid factor step comprises dispensing gravimetric measurement and a series of calculations Refer to Calibration on page 63 manifold A common coil block of propylene into which the eight dispensing valve tubes are inserted Figure 3 12 predispense The volume that is automatically dispensed before filling each plate prime Filling the liquid path with solution to be dispensed priming vessel An external waste container of blue propylene for excess priming liquid which can also be equipped with a tube drain or cap Figure 3 14 protective cover The blue sliding cover that is pulled over the dispensing valve head when the instrument is on Figure 2 2 To pause operation and hold the instrument on standby the protective cover is pushed in to release the pressure remote control Running mode allowing a remote computer to operate the dispenser Refers to use in automated systems tip wash purge A sequence of operations to remove persistent air bubbles and debris from the hosing solenoid dispensing valves and to open clogged dispensing valves USB Universal serial bus Thermo Scientific Multidrop Combi nL User Manual 105 Glossary 106 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Index 1 1536 well plates 13 14 52 91 3 384 well plates 13 52 91 9 96 well plates 52 74 A accessories 6 19 20 88 101 accu
32. alculated and transferred with FILLit Software For more information refer to the FILLit Software for Multidrop Combi nL User Manual Cat no N07173 The Multidrop Combi nL microplate dispenser has several operational advantages e Flexible low volume range of 50 nl 50 pl e Flexible dispensing height of 5 55 mm e High speed dispensing of column row and well specific volumes e Superior precision covering the whole volume range e Easy to use visual user interface e PC software for increased flexibility e Backtflush feature for reagent recovery e Excellent robot compatibility Thermo Scientific Multidrop Combi nL User Manual 15 Introduction to the Multidrop Combi nL Advantages of using Multidrop Combi nL 16 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Thermo Fisher Scientific Chapter 2 Functional Description Instrument This section shows the front and back views of the Multidrop Combi ay 0 ut nL instrument Front view The front view of the Multidrop Combi nL instrument is shown in Figure 2 2 Light indicator Display Protective cover Dispensing valve head Dispensing valve 8 i _ Reagent reservoir gt Priming vessel Plate carrier Figure 2 2 Multidrop Combi nL front view Thermo Scientific Multidrop Combi nL User Manual 17 Functional Description Instrument layout The close up of the Multidrop Combi nL keyboard and display is shown
33. anism cannot move Too many protocols saved Predispensing undone Priming vessel missing Protective cover not in place When tubes or plugs are not fastened properly Action Power off on or contact service Power off on or contact service Power off on or contact service Delete protocols that are not used Press the PRIME button Insert the priming vessel Pull the protective cover over the dispensing valve head Check that the tube attachments and caps are properly tightened Examples of a few error messages that appear in the Multidrop Combi nL internal software are shown below Thermo Scientific Multidrop Combi nL User Manual 97 Troubleshooting Guide Error and warning codes Settings Options Missing prime vessel Press OK to acknowledge the error and then insert the priming vessel Denr eTA TT E Main lt Settings Options Protective cover not in place Press OK Press OK to acknowledge the error and then pull the protective cover over the dispensing valve head 98 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Thermo Fisher Scientific Hazards Electrical Troubleshooting Guide Hazards vati mete settings Optior ea sine th Pressure failure Press OK Press OK to acknowledge the error and then tighten the tube attachments and caps properly There are three warning messages that can appear in the main view of
34. ced level options there are two extra menus Settings and Options You can navigate between these three menus using the Left and Right arrow keys The main view row is either colored active or uncolored inactive All the descriptive icons used in the main view are shown in Table 4 2 on page 35 The info text bar shows explanatory information on how to proceed and which keys to use Navigating This section visualizes navigation in the Multidrop Combi nL user interface The main view changes according to the selections you make either with the Up or Down arrow keys or the OK button The available buttons and their function are shown on the info text bar Active item 5Onl 50 0pl 1 000 OK Volume and Liquid Available buttons and Volume lt gt Liquid OK Accept their function The color of the items for example the icon and main view row in the main view changes when they are selected active inactive To move from one menu to another make sure you are in the main view of one of the menus and use the Left and Right arrow keys The main views of each menu tab are shown below Seumgss Options Device information RS 232 Default protocol 384 lo Instrument settings Liquid factor OK Plate type OK Speed OK Device information 34 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Routine Operation Control panel Table 4 2 Icons in the main view Menu Icon Function
35. contamination procedure on page 84 V Refit the transport lock when relocating the instrument or sending it for service See How to refit the transport lock on page 86 vV depending on the laboratory conditions and the use and configuration of the instrument when necessary Regular and For reliable daily operation keep the instrument free of dust and liquid spills To prevent unnecessary wear or hazards follow the routine and preventive service procedures described below at regular intervals maintenance Clean the outside of the instrument periodically with a cloth dampened 72 Thermo Scientific Multidrop Combi nL User Manual with water or a mild detergent e g dishwashing liquid or 70 ethanol when necessary Immediately wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage Abrasive cleaning agents are not recommended because they are likely to damage the plastic instrument cover Caution Always switch the instrument off and disconnect the power cable before cleaning the dispenser A Caution The surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the manufacturer Do not expose the surfaces to concentrated acids or concentrated alcohols for prolonged periods of time as damage may occur A It is recommended to service the instrument at least yearly Refer to Service contracts on page 88 If
36. d remove the reagent filter and the tube from inside the cap of the reagent reservoir Figure 5 27 Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 77 Maintenance Regular and preventive maintenance Figure 5 27 Removing the reagent filter with tube 78 Thermo Scientific Multidrop Combi nL User Manual Place a disposable reagent basin filled with detergent for example filtered 1 Micro 90 or equivalent under the dispensing valves so that the liquid is in contact with the valves Figure 5 24 Press the EMPTY button for several minutes Place another disposable reagent basin now filled with distilled and filtered laboratory grade water under the dispensing valves so that the liquid is in contact with the valves cf Figure 5 24 Press the EMPTY button for several minutes Clean the reagent reservoir Replace the 43 um reagent filter and the tube into the cap of the reagent reservoir Add distilled and filtered laboratory grade water into the reagent reservoir Close the cap Press the PRIME button to rinse the tubing Try to dispense Repeat this entire procedure Steps 2 to 7 if the dispensing valve is still leaking Now the instrument is ready for dispensing Thermo Fisher Scientific Maintenance Regular and preventive maintenance How to clean a clogged Foreign particles can cause partial or complete clogging of a dispensing dispensing valve valve Partial clogging can be obs
37. d Sets Cat no D05871 document Contact your distributor or local Thermo Fisher Scientific representative to obtain the document in PDF format eT iade Active Press STOP to eyit The instrument has two alternative computer interfaces RS 232 and USB Note It is recommended to use USB to improve the reliability of traffic in robot applications A Note The interface to use is selected from the Multidrop Combi nL user interface A Thermo Scientific Multidrop Combi nL User Manual 93 Technical Specifications Remote control to Multidrop Combi nL 94 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Thermo Fisher Scientific O amp As Chapter 7 Frequently Asked Questions QI What liquid is recommended for washing the Multidrop Combi nL hosing A1 It is recommended that you use distilled and filtered laboratory grade water and or a detergent solution for example filtered 1 Micro 90 followed by distilled and filtered laboratory grade water Q2 Do particles in the reagent affect dispensing A2 Yes It is recommended that you ensure that there are no particles gt 40 um in the reagent to avoid blockage of the dispensing valves Filter all reagents detergents solutions and water before use to remove any solid particles present Q3 What materials come into contact with liquids to be dispensed A3 The standard Multidrop Combi nL materials that come into contact with liquids to be dis
38. e instrument and the dispensing area periodically v see Regular and preventive maintenance on page 72 Clean the tubings when necessary See How to clean the tubings on v page 3 Clean the reagent reservoir when necessary See How to clean the v reagent reservoir on page 82 Clean the plate carrier when necessary See How to clean the plate v carrier on page 83 Clean the priming vessel when necessary See How to clean the V priming vessel on page 83 Maintain the dispensing valves daily See Daily maintenance on page v 76 Maintain the dispensing valves periodically See Daily maintenance on page 6 Clean the leaking or clogged dispensing valves tips when necessary see How to clean a leaking dispensing valve on page 77 and How to clean a clogged dispensing valve on page 79 Replace the dispensing valve when necessary See Replacing a dispensing valve on page 79 Continued Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 71 Maintenance Regular and preventive maintenance Cont Procedure gt gt F E S A gt Replace the tubing set and a manifold if necessary See Replacing a tubing set and a manifold on page 81 Ensure proper shutdown See Shutdown on page 68 v service the instrument regularly v Decontaminate the instrument when relocating the instrument or v sending It for service See De
39. ect the valve tube ends to the dispensing valves Figure 5 26 Ny g L v 1 Figure 5 26 Reconnecting a dispensing valve tube end 12 Finally prime once again with distilled and filtered laboratory grade water The Multidrop Combi nL liquid path components can be disinfected with isopropanol 10 bleach for example sodium hypochlorite or 70 ethanol by running the disinfectant through the liquid path To obtain optimal performance and a maximum useful life for the dispensing valves it is important that the recommended cleaning maintenance instructions are followed If the dispensing valve is leaking or clogged refer to How to clean a leaking dispensing valve on page 77 and or How to clean a clogged dispensing valve on page 79 Routine maintenance of the dispensing valves covers the sections described below The basic maintenance procedure should be performed regularly and on a daily basis to ensure proper dispensing valve operation 1 Filter all reagents detergents solutions and water before use to remove solid particles 76 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Maintenance Regular and preventive maintenance 2 Rinse the tubings with distilled and filtered laboratory grade water always after using the instrument a Warning Before a prolonged standby run the tubings dry to avoid microbial growth from developing A Tip washing To routinely remo
40. een changed without any liquid change the factor may be valid through the dispensing range 50 nl 50 pl A new accuracy check should be done after the dispensing volume is switched a if the accuracy check and Liquid factor adjustment have been made after the liquid change and b if the physical properties viscosity surface tension etc of the new liquid differs from the one in the calibration curve used However in this case it is recommended to create a new calibration curve by using FILLit Software A For more information refer to the FIL Lit Software for Multidrop Combi nL User Manual Cat no N0O7173 The Multidrop Combi nL screen saver shown below appears when the instrument has not been used for 20 minutes You can close the screen saver and return to the previous display by pressing any key sc LEM Ftr tc To shut down the Multidrop Combi nL Warning Remove any microplates still on the instrument Dispose of all microplates and strips as biohazardous waste A 1 After use remove the reagent from the tubings Press the EMPTY button to back flush the reagent if needed However just to empty the tubings press the PRIME button Rinse the tubings with distilled and filtered laboratory grade water by emptying the reagent reservoir rinsing it first with filtered laboratory grade water and then by topping it up with water Alternatively you can use a spare reagent reservoir supplied with the instrument and f
41. efault duration is 0 s Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 47 Routine Operation Protocol settings lt gt Dispensing offsets Use the Right and Left arrow keys to select the speed of the shake The available speed settings are Low Med Medium and High The default speed is Low The speed settings with regard to the movement distance in millimeters mm and the frequency cycles per second in hertz Hz are presented in Table 4 5 Table 4 5 Speed settings vs distance movement and frequency Speed Movement distance mm Frequency Hz Low 2 10 Medium 1 15 High 1 20 Press OK This main view row includes both the dispensing height and the dispensing offset functions Go to the Settings menu Select the Offsets row No e Shaking my e nue Fente a i Offsets OK Offsets Press OK The track mechanism and the dispensing valve head move to the setting position When you adjust the height the dispensing valve head moves next to the plate On the other hand when you adjust the X Y offset the dispensing valve head moves over the first column of the plate but the default is always 1 mm above the plate Adjust the optimal offset values visually 48 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Routine Operation Protocol settings et a a i 4 Main Fany Select OK Accept Use the Up and Down arrow keys to select
42. el 1 Unfasten the priming vessel by sliding it off towards yourself Figure 5 32 Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 83 Maintenance Disposal of materials Disposal of materials Decontamination procedure A Figure 5 32 Unfastening the priming vessel 2 Rinse or wash the priming vessel with water or detergent when necessary 3 Fasten the dried priming vessel by sliding it back over the snap lock Figure 3 8 The priming vessel is made of blue polypropylene PP and can be autoclaved The autoclaving conditions of the priming vessel are 1 bar pressure at 121 C for 20 minutes Follow laboratory and country specific procedures for the disposal of biohazardous or radioactive waste Refer to local regulations for the disposal of infectious material Warning The samples can be potentially infectious Dispose of all used plates strips disposable gloves syringes disposable tips and so on as biohazardous waste A If you have spilled infectious agents carry out the decontamination procedure Warning The decontamination procedure should be performed by authorized and trained personnel in a well ventilated room wearing disposable gloves protective glasses and clothing A Decontamination should be performed in accordance with normal laboratory procedures Any decontamination instructions provided with the reagents used should be followed 84 Thermo Scientific Multidrop
43. er Scientific Thermo Scientific Multidrop Combi nL User Manual 23 Installation Installation setups Figure 3 11 Connecting the hosing to the reagent reservoir 3 Detach the red manifold transport plug assembly Figure 3 12 A 4 Fit the reagent reservoir hosing on top of the bottle to the manifold Figure 3 12 B of the dispensing valves It is enough to insert the hosing only halfway onto the manifold tube connector Finally insert the hosing into the groove intended for it Figure 3 13 A B Figure 3 12 Close up of the manifold Caution To detach the manifold tube connector pull the hosing off Do not twist the hosing A 24 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Operational Thermo Fisher Scientific check A Installation Operational check i i Figure 3 13 Reagent reservoir connected to the instrument Complete the following procedure to confirm the correct functioning of the dispenser prior to normal use 1 Connect the instrument to a correctly installed line power outlet which has a protective conductor also called earth or ground Switch the instrument on using the mains switch Figure 2 4 Warning Interruption of the protective conductor inside or outside the instrument or disconnection of the protective conductor terminal may make the instrument dangerous A e Ifthe instrument starts properly e The display lights up e The plate carrier moves
44. erial number Q10 What is the orifice of the dispensing valve A10 The orifice is 100 pm QII Do you need to recalibrate the instrument when a dispensing valve is changed A11 No you do not have to recalibrate the instrument because the valve performance is so consistent Q12 I have an old spare valve which does not have any valve correction factor enclosed Can I still use it A12 Yes you can use it Old spare valves do not have this correction factor and the default value 0 is alright If an old valve replaces the newer version the correction factor needs to be set to value 0 96 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Error and warning codes Error Plate X position error Plate Y position error Z position error No more memory for storing user data Not primed Missing prime vessel Protective cover not in place Pressure failure Thermo Fisher Scientific Chapter 8 Troubleshooting Guide Note Do not use the instrument if it appears that it does not function properly A When an error is detected the current operation is terminated After an error it is best to abort the current run and restart from the beginning after the problem is fixed The Multidrop Combi nL internal software has the following error messages Table 8 8 Table 8 8 Error messages reported Cause Plate carrier cannot move Plate carrier cannot move Dispensing valve lifting mech
45. erved when the dispensing spray starts to slant or a droplet forms on the valve end during dispensing If the dispensing valve is completely clogged no fluid comes through the valve To clean a clogged dispensing valve 1 Follow the Steps 1 to 7 above in How to clean a leaking dispensing valve on page 77 2 Repeat this entire procedure Steps 2 to 7 if the dispensing valve is still clogged See Step 1 above If the dispensing valve is still clogged after the cleaning attempts replace the dispensing valve For more information refer to Replacing a dispensing valve on page 79 Replacing a dispensing If the cause for the dispensing valve change is clogging of the valve first valve clean the tubings and the reagent reservoir For more information refer to How to clean the tubings on page 73 and How to clean the reagent reservoir on page 82 To replace a dispensing valve 1 Release the pressure by pressing the PRESSURE button or by pushing the protective cover in The indicator light is off when the pressure has been totally released 2 Detach the flexible dispensing valve tubing Figure 5 25 3 Unfasten the valve screw with the 1 5 mm hexagonal screwdriver supplied Figure 5 28 Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 79 Maintenance Regular and preventive maintenance Figure 5 28 Unfastening the dispensing valve 4 Pull the dispensing valve downwa
46. est option is default for each plate type However you may change the dispensing direction if necessary Go to the Settings menu Select the Dispensing direction row Man Settings d OFF g3 Shaking hra Offsets Ban Settings E Row wise direction Column wise direction Select OK Accept Thermo Scientific Multidrop Combi nL User Manual 51 Routine Operation Protocol settings Use the Up and Down arrow keys to set the dispensing direction row wise or column wise Note that the whole action is only effective when 384 or 1536 well plate types are selected Press OK The Dispensing direction function is useful in assays where reaction times are critical With the 96 well plate selection the columns with 8 wells are filled starting from column 1 according to the selected columns after which the plate carrier returns to the home position and the dispensing valve lifting mechanism to the up position Row wise and column wise dispensing show no difference in 96 well plate dispensing With the 384 well plate selection the columns with 16 wells are filled in two phases first every other row A C E G O starting from the columns selected and then the dispensing valve head carrier shifts sideways so that the remaining rows B D F H P are filled in the opposite order If column wise dispensing is used it shifts the dispensing valve head carrier first sideways filling 16 wells a
47. et in the x axis and the Up and Down arrow keys to set it in the y axis direction While you change the offset values the dispensing valve head moves accordingly The offset is set in 0 05 mm increments in both the and directions The maximum offset value depends on the selected plate type Press OK If the OK button is not pressed within 60 seconds the adjustments are disregarded and the main view returns to the Settings menu y axis the dispensing valve head moves x axis the plate carrier moves You can change the predispensing volume the volume that is automatically dispensed before the start of plate filling if necessary The default predispensing value is 100 nl 2 x 50 nl Some more viscose solutions used may sometimes require extra predispensing Go to the Settings menu Select the Predispense Prime row Settings oo Speed J Ke Shaking OFF icla Offsets Predispense 100nl C Direction OK Predispense Press OK 50 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Dispensing direction Thermo Fisher Scientific Routine Operation Protocol settings Predispense volume OK Accept Use the Up and Down arrow keys to set the predispensing volume The volume ranges from 0 to 200 nl in 50 nl increments Note that the predispensing value can be zero if predispensing is not required Press OK The row wise dispensing direction being the fast
48. fic technical service department for more details 88 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Disposal of the Thermo Fisher Scientific Instrument Maintenance Disposal of the instrument If the Multidrop Combi nL has to be disposed of follow the guidelines below Warning Decontaminate the instrument before disposal Refer to cc e 329 e e Decontamination procedure on page 84 about decontamination A Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equipment and waste The procedures vary by country Pollution degree 2 see Safety specifications on page 92 Method of disposal Electronic waste Contaminated waste Infectious waste Regarding the original packaging and packing materials use the recycling operators known to you For more information contact your local Thermo Fisher Scientific representative Thermo Scientific Multidrop Combi nL User Manual 89 Maintenance Disposal of the instrument 90 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Chapter 6 Technical Specifications General Thermo Fisher Scientific reserves the right to change any specifications g without prior notice as part of our continuous product development specifications program The general
49. g a dispensing cycle If it is necessary to stop the operation of the equipment press the STOP button on the control panel Infectious samples and corrosive fluids are commonly used with this equipment The hands off nature of the system allows the user to dispense into the reaction wells without getting into direct contact with these fluids However the wells that have been in contact with potentially hazardous fluids must be handled with utmost care Always wear hand and eye protections as well as corrosive resistant laboratory coats Warning Observe normal laboratory procedures for handling potentially hazardous samples A Whenever it is likely that the protection against safety hazards has been impaired make the instrument inoperative and secure against any unintended operation The protection is likely to be impaired if for example the instrument e Shows visible damage e Fails to perform the intended functions e Has been subjected to prolonged storage under unfavorable conditions e Has been subjected to severe transport stresses 100 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Multidrop Combi nb List of accessories Thermo Fisher Scientific Chapter 9 Ordering Information Contact your local Thermo Fisher Scientific representative for ordering and service information Ordering information codes are presented in Table 9 9 and Table 9 10 Table 9 9 Instrument catalog
50. ill it with filtered laboratory grade water 68 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Thermo Fisher Scientific Emergency situations Routine Operation Emergency situations 2 If there is only a short break in dispensing no washing or cleaning of the tubes is necessary depending of course on the liquid used For example crystallizable buffer solution requires immediate washing and cleaning of the tubes after use 3 If there is a longer break rinse the tubings with distilled and filtered laboratory grade water Refer to the sections under Regular and preventive maintenance on page 72 Warning Before a prolonged standby run the tubings dry to avoid microbial growth from developing A 4 Switch the Multidrop Combi nL off by pressing the mains switch Figure 2 4 at the left of the back panel of the instrument into the OFF position The pressure is released Note To pause operation and hold the instrument on standby just push the protective cover in to release the pressure A 5 Wipe the instrument surfaces with a soft cloth or tissue paper moistened with deionized distilled water a mild detergent e g dishwashing liquid or soap solution 6 Ifyou have spilled infectious agents on the dispenser disinfect with 70 alcohol or some other disinfectant see Decontamination procedure on page 84 In case there is any abnormal situation during operation such as fluids spil
51. information R 232 Default protocol 384 lo Instrument settings Liquid factor OWK Liguid factor Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 63 Routine Operation Instrument options Factor START Dispense OK Accept You set the Liquid for example Water in the Volume and Liquid window of the Main menu Refer to Dispensing volume and liquid on page 39 Five liquids are factory set Dispense the selected volume measure the weight of the dispensed liquid and calculate the liquid factor Refer to Factor calculation on page 66 Use the Up and Down arrow keys to select the factor for the liquid used The default settings for the liquid the dispensing speed and the dispensing volume are presented in the Factor window Use the Left arrow key to check the dispensing volume Use the Right arrow key to open the Quick Guidance window vo ff Soe erie OK Return Press OK Accuracy check To perform an accuracy check and factor calculation see Factor calculation on page 66 follow the procedures below e Go to the Options menu Select the Liquid factor row 64 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Routine Operation Instrument options ef so Options Device information R 232 Default protocol 384 lo Instrument settings Liquid factor OWK Liguid factor Expected volume to be dispensed
52. internal software 37 using the keyboard and display 14 18 32 65 91 100 V vacuum 13 14 23 29 31 valve open time 14 30 105 viscosity 14 68 volume 13 14 15 30 38 39 41 42 43 44 46 50 51 63 64 65 66 67 105 range 13 14 15 51 67 W warning messages 99 warranty 19 20 X X Y offset 48 50 Thermo Scientific Multidrop Combi nL User Manual 109 110 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Notes Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 111 Notes 112 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Thermo Fisher Scientific Oy Ratastie 2 PO Box 100 FI 01621 Vantaa Finland www thermoscientific com N07171
53. ir or replacement If any parts are damaged contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy Move the packed instrument to its site of operation To prevent condensation the instrument should be left in its protective antistatic plastic wrapping until the ambient temperature has been reached Unpack the Multidrop Combi nL instrument and accessories carefully with the arrows on the transport package pointing upwards Remove the instrument from the package and place it on a level surface Refer to the enclosed packing instructions The following notes and instructions are sent with the instrument and are immediately available when you open the package e Unpacking instructions e Warranty Certificate card e Packing instructions Packing list e Multidrop Combi nL User Manual and Quick Reference Guide e Transportation discrepancy report Thermo Scientific Multidrop Combi nL User Manual 19 Installation Requirements Retain the original packaging and packing material for future transportation The packaging is designed to assure safe transport and minimize transit damage Use of alternative packaging materials may invalidate the warranty Also retain all instrument related documentation provided by the manufacturer for future use Requirements When you set up your Multidrop Combi nL avoid sites of operation with excess dust vibrations strong magnetic fields direct sunlight or UV light draft
54. king Conformit Europ ene European Conformity CE Marking on a product is a manufacturer s declaration that the product complies with the essential requirements of the relevant European health safety and environmental protection legislations the product may be legally placed on the market and thus the CE Marking ensures the free movement of the product within EU current protocol In standalone mode the current protocol is the protocol affected by changes through the user interface In remote mode it is the protocol affected by computer commands decontamination Removal or neutralization of radiologic bacteriological chemical or other contamination dispense To distribute liquid into the wells of the preselected strips or microplate dispensing height The valve position on top of the microplate The distance between the bottom surface of the plate carrier to the tip of the dispensing valve Table 4 3 dispensing valve A normally closed 2 2 way fluid valve for high precision and high speed dispensing of liquids EIA Enzyme immunoassay empty To draw back to the liquid bottle all the liquid in the hoses and valves EN European Norm EU European Union Thermo Fisher Scientific home position The plate carrier is positioned to the furthest right of the transfer rails IEC International Electrotechnical Commission liquid factor Standalone calibration of the liquid used at a set volume point The factor varies
55. ling inside the instrument follow the steps below 1 Switch OFF the instrument Figure 2 4 2 Unplug the instrument immediately from the power supply 3 Carry out appropriate corrective measures However do not disassemble the instrument 4 Ifthe corrective measures taken do not help contact authorized technical service or your local Thermo Fisher Scientific representative Thermo Scientific Multidrop Combi nL User Manual 69 Routine Operation Emergency situations 70 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Chapter 5 Maintenance Maintenance This chapter on maintenance contains an outline of the checklist tasks procedures mentioned in the checklist below Table 5 6 Contact local authorized technical service or your local Thermo Fisher Scientific representative for assistance if necessary Table 5 6 Maintenance checklist Procedure Weekly Monthly Yearly Keep the instrument free of dust See Regular and preventive maintenance on page 72 lt I oN Daily Wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces immediately to prevent damage and wipe with deionized distilled water See Regular and preventive maintenance on page 72 If any surfaces have been contaminated with biohazardous material v disinfect with a mild sterilizing solution See Regular and preventive maintenance on page 72 Clean the case of th
56. markings appear in this user manual Warning Risk of electric shock A Warning Biohazard risk A Warning Risk of injury to the user s A Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application A Note Marks a hint important information that is useful in the optimum operation of the system or an item of interest A Thermo Scientific Multidrop Combi nL User Manual 5 About This User Manual Instrument safety and guidelines for use Instrument safety and guidelines for use 6 Thermo Scientific Multidrop Combi nL User Manual Always follow basic safety precautions when using the Multidrop Combi nL to reduce the risk of injury biohazardous contamination fire or electric shock Read this user manual in its entirety prior to operating the instrument Failure to read understand and follow the instructions in the manual may result in damage to the instrument injury to laboratory and operating personnel or poor instrument performance Observe all Warning Caution and Note statements as well as safety symbols and markings on the instrument and in the documentation The Multidrop Combi nL is intended for laboratory research use only Observe proper laboratory safety precautions such as wearing protective clothing and following approved laboratory safety procedures Use of the Multidrop Combi nL in ways other than those described in the documentatio
57. meters AV Selecting columns and rows Selected wells Volume value box EMPTY Clr all 34 gt Move OK Fill Clear Press the OK button for 0 5 seconds The Volume value box is now activated bright red Adjust the dispensing volume of the row where the cursor is located using the Up and Down arrow keys Volume OK Accept STOP Escape Press OK after selecting the desired volume Use the Down arrow key and select Accept and press OK after selecting the row volumes you wish You can press the STOP button to exit the volume selection without making any changes You can first select columns of a microplate with a certain volume and then select rows of the same microplate with another volume or vice versa Where the selected columns and rows cross each other the wells will be filled with the row or column volume that was last selected when such a protocol is executed Refer to the example plate in Figure 4 21 where the 100 nl row selection overwrites the 50 nl column selection made first In general be careful if you choose to carry out cross dispensing where selected wells are concerned 44 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Protocol settings Thermo Fisher Scientific Routine Operation Protocol settings Figure 4 21 Example of dispensing with selected wells This section describes the additional parameters that supplement the plate type you have selected
58. ming Vessel vans pate ceetie enced 83 Disposal or TACHA S anire da nunece need oieebalatenneiataaraeteren 84 Decontamination procedures wnnciungh c cab intiuneta ue Manaielgi 84 How TOmeMt he transporto R astora n TE EEN 86 PACKING TOP seiv Ceea e E 87 SOVICE CONTACTS iaa E EEE 88 Disposal of the instrument sssessesssssssssssrssssresseresssresssresssreesseresses 89 Technical Specifications ccssssssssesssssseeeesesesseeeesseseseeenaeaees 91 CFS oo RS ole lee he nee epee nnn Ene nr an ere ear i ee 91 Salely SPEC MICALIOING cates ices E E R O 92 LI A a a N O O OR T 92 In conformity with the requirements sssesessesssssresssresssrresseresses 92 Remote control to Multidrop Combi nL eeeeeeeeneeeeeeneeeees 93 Frequently Asked Questions cccscsssssssssseeeesessseseeesesesenennaeaes 95 GAS E AE OET E E A T 95 Troubleshooting Guide c ssssscssssessesesssesssesesssseesnseesetaeseeaess 97 Errorand warnine CodeS ssiri onena 97 Faza a T E S A A TS 99 e Ceara co ae A A A E ator Cerner es 99 Mechanical yenen A E NS 100 Favona aknan a a 100 Detectsand abnormal Stresses 5 iixsacecndsctvncarscaetansiaevodetennttedsaiaedies 100 Ordering InformatiOn csesssessessesessesesesesesseseeseseesnseeneanseeess 101 Muliarop Comb sssini a E 101 Listor ac cessor eee ce Tee eee een Re ener rene Semen TS 101 Certificate of Decontamimation csssssssssseeesseeeeseeeesenseesnenseenees 103 Therm
59. n supplied with the equipment may result in injury to persons or damage to the property Avoid unintended use of the equipment for example using incompatible materials making unauthorized modifications using incompatible or damaged parts using unapproved auxiliary equipment or accessories or operating equipment in excess of maximum ratings Do not touch the dispensing valves or the tubing inlet to ensure trouble free dispensing Thermo Fisher Scientific assumes no liability for the use of third party dispensing valves Preventative maintenance instructions should be followed closely to keep the instrument in the best condition for maximum reliability A poorly maintained instrument will not give the best results Thermo Fisher Scientific Chapter 1 Chapter 2 Chapter 3 Chapter 4 Thermo Fisher Scientific Contents ende dii i erea A EEA 3 How to use this user manual ssrersraie isini aE oi E 3 For more morma oN e E 3 Safe symbolsand Markings iscsi 4 Safety symbols and markings used on the Multidrop Combi nL 4 Warning and other markings used in the documentation 5 Instrument safety and guidelines for use esssssesessessseessessersserssseses 6 Introduction to the Multidrop Combi nu 13 MEA 6 NS rt ls Ac te dat Ti 14 Punciple o COC ra tOl seier a E tunedin 14 Pressute vac n syste Mero 14 Dispensing appl earions ssena i 14 CANES O EE E T E E E EE E A S 15 Advantages of using Multidrop Combi nL
60. nd then the plate carrier moves dispensing over the next column With the 1536 well plate selection the columns with 32 wells are filled in four phases first every fourth row A E I M AC and then the dispensing valve head carrier shifts sideways above the rows B F J N AD which are then filled in the opposite order After three shifts the remaining rows D H L P AF are filled Column wise dispensing shifts the dispensing valve head carrier sideways three times filling 32 wells and then the plate carrier moves the dispensing valve head over the next column 52 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Routine Operation Running a protocol Running a Note that multistep protocols that is protocols with several steps e g Dispense Shake Empty etc can be run with FILLit Software for protoco Multidrop Combi nL You can also download protocols with one dispense step and an additional shake step to the instrument For more information refer to the F LLit Software for Multidrop Combi nL User Manual Cat no N07173 To run a dispensing protocol follow the steps below Note Do not dispense extensively without any liquid to avoid overheating of the pump e Check that there is always enough liquid in the reagent reservoir and ensure that the reagent filter is below the liquid level Use a test tube placed inside the reagent reservoir if a limited amount of liquid is available
61. nifold transport plug assembly Figure 3 12 e Use the original packaging to ensure that no damage occurs to the instrument during shipping Any damage incurs additional labor charges e Pack the instrument according to the enclosed packing instructions e Replace the foam absorbers of the instrument and lower the dispenser into the carton Refer to the packing instructions e Place the accessories box into the carton and fit this between the foam absorbers on top of the instrument e Inform about the use of hazardous materials Enclose a dated and signed Certificate of Decontamination see Appendix A Certificate of Decontamination both inside and attached to the outside of the package in which you return your instrument or other items e Enclose the instrument serial number as well as the return authorization number RGA given by your local Thermo Fisher Scientific representative e Seal the box carefully and securely Make sure all required information is clearly indicated on the outside of the carton and enclosed as instructed Refer to General specifications on page 91 for details on storage and transportation temperatures Service Itis recommended to maintain and service the instrument regularly t t every 12 months on a contract basis by the manufacturer s trained contracts service engineers This ensures that the product is properly maintained and gives trouble free service Contact the Thermo Fisher Scienti
62. number Code 5840400 Instrument System Multidrop Combi nL 100 240 V 50 60 Hz Table 9 10 Codes for accessories Code NO7171 N07173 5188020 N07271 N05843 N07494 N07493 N07497 N07489 N07490 N07491 N07492 N07495 9510027 1210550 1210520 2305290 N04001 0300033 Continued Item Multidrop Combi nL User Manual FILLit Software for Multidrop Combi nL User Manual FILLit Software for Multidrop Combi nL Priming vessel Multidrop Combi nL Priming vessel tube assembly and cap Dispensing valve Multidrop Combi nL Reagent filter 43 um PTFE AISI 316 5 pcs box Reagent reservoir 250 ml Nalgene propylene w o tube connectors Reagent reservoir 250 ml polypropylene accessory assembly incl reagent reservoir reagent filter tubings and manifold Reagent reservoir 1000 ml polypropylene accessory assembly incl reagent reservoir reagent filter tubings and manifold Tubing set and manifold for reagent reservoir 250 ml Tubing set and manifold for reagent reservoir 1000 ml Tubing assembly from reagent reservoir to pressure vacuum system Reagent basin 60 ml 5 pcs box Cord Mains EURO Cord Mains UL CSA Serial cable F9 F25 Cord USB 2 0 A B 1 8 2 0 m Tool 3mm DIN911 Allen key Handled Thermo Scientific Multidrop Combi nL User Manual 101 Ordering Information List of accessories Cont Code Item N07523 Tool 1 5mm DIN911 Allen key Zn 102 Thermo Scientific Multidrop Combi nL User Manual Thermo Fi
63. o Scientific Multidrop Combi nL User Manual 9 Figures Figures Figure 1 1 Multidrop Combi nL microplate dispenser 0 0c eee 13 Figure 2 2 Multidrop Combi nL front VieW cece cece eceeeeeeeeeen 17 Figure 2 3 Close up of the Multidrop Combi nL keyboard and display 18 Figure 2 4 Multidrop Combi nb back VieW uw ccc cecceeeeceeteeeeeeereeen 18 Figure 3 5 Transport lock and transport lock tag present 0c cee 21 Figure 3 6 Removing the transport IOCK ccceceeeeeceescscessereetestesteneenens 21 Figure 3 7 Transport lock storage o oo cecececcccessessesescessestessneatestesseneenens 22 Figure 3 8 Inserting the priming vessel eeen 22 Figure 3 9 Removing the red luer plugs 23 Figure 3 10 Connecting the hosing to the pressure vacuum system 23 Figure 3 11 Connecting the hosing to the reagent reservoir 24 Figure 3 12 Close up of the manifold o oo eee ececeseceseteteseeeereeen 24 Figure 3 13 Reagent reservoir connected to the instrument 0 0 25 Figure 3 14 Priming vessel inserted Into Place ocen 26 Figure 4 15 Principle of the pressure vacuum dispensing system 29 Figure 4 16 Eight dispensing valves inserted into the manifold 29 Figure 4 17 Reagent reservoir connected 31 Figure 4 18 Removing the reagent filter 32 Figure 4 19 Keyboard of the Multidrop Combi nL o s 32 Figure 4 20 Main view on the display
64. of the Multidrop Combi nL 33 Figure 4 21 Example of dispensing with selected wells 45 Figure 4 22 Calibration curve vs factor and volume changes 67 Figure 5 23 Removing the reagent filter 74 Figure 5 24 Cleaning the tubings using a reagent basin 0cccee eee 74 Figure 5 25 Detaching a dispensing valve tube end scen 15 Figure 5 26 Reconnecting a dispensing valve tube end 76 Figure 5 27 Removing the reagent filter with tube s n 78 Figure 5 28 Untastening the dispensing valve o n 80 Figure 5 29 Pulling the dispensing valve GownWalds 0cc cccceeeeee 80 Figure 5 30 Replacing the dispensing valve ccccceccesceseeeseseeseeterees 81 Figure 5 31 Replacing the tubing set and manifold 0 0 0 0 cece eee 82 Figure 5 32 Unfastening the priming vessel 84 Figure 5 33 Transport lock storage location s e 86 Figure 5 34 Refitting the transport 00k 87 Figure 5 35 Transport lock fastened neeerrrreenn 87 10 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Thermo Fisher Scientific Tables Tables Table 4 1 Calibration point vs dispensing volume and dispensing times 30 Table 4 2 Icons in the MAIN VIQW ccecceccececescessessetescessestetestestestenteneaees 35 Table 4 3 Default parameter values for different plate types 0 38 Table 4 4 Relationship between dispensing speed and press
65. olume in nanoliters 1000000 Dispensed liquid mass in grams Density of the liquid g cm e Set volume in nanoliters Expected total volume in the dispensed strip at the selected volume setting and calibration Main menu Note Calibration curves are calculated from gravimetric results and volume calculations are always carried out by using density correction so real volumes are obtained The difference between liquid mass and volume in water is around 0 3 at normal room temperatures and may be omitted in the accuracy check but with liquids having a different density to water the mass conversion to real volumes should also be carried out in the accuracy check step A Example Accuracy check with water at 200 nl volume set 1 Measured liquid mass 0 1815 g 2 Conversion to volume nanoliters 1000000 0 1815 0 9969 water density at 22 C 182064 nl 3 ACCURACY 100 182064 nl 179200 Expected volume 179200 1 6 For more information refer to the FIL Lit Software for Multidrop Combi nL User Manual Cat no N07173 Factor calculation To perform a factor calculation 1 Calculate the correction factor by dividing the expected dispensing volume in the factor formula by the measured volume as follows Factor Expected volume nl Measured dispensed volume nl Factor in the previous example would be 179200 nl 182064 nl 0 984 which means that the instrument dispenses 0 984 times liquid compared to the val
66. p to 31 C decreasing linearly to 50 relative humidity at 40 C Mains supply fluctuations 10 from nominal Installation category overvoltage ll according to IEC 60664 1 see Note 1 category Pollution degree 2 according to IEC 60664 1 see Note 2 Thermo Fisher Scientific Remote control to Multidrop Combi nb Thermo Fisher Scientific E Technical Specifications Remote control to Multidrop Combi nL Note 1 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 2 The pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only nonconductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation A The Multidrop Combi nL can be used as part of an automated system When the Multidrop Combi nL is in remote mode the main view is as shown below For more details refer to the Multidrop Combi nL Remote Control Comman
67. pensed are e Reagent reservoir polypropylene PP e Reagent filter stainless steel AISI 316 and polytetrafluoroethylene PTFE Teflon e Tubings chemical resistant tubing e Tube connector polypropylene PP e Manifold polypropylene PP e Dispensing valve stainless steel polyetheretherketone PEEK sapphire and ruby Q4 What should you do if the liquid foams during dispensing A4 Some liquids foam more easily than others One option is to reduce the speed that is the pressure There are five dispensing speeds available 1 to 5 Thermo Scientific Multidrop Combi nL User Manual 95 Frequently Asked Questions Q amp As Q5 Do you need specific plate adapters for the Multidrop Combi nL A5 No the Multidrop Combi nL has a fixed plate adapter and does not use any external plate adapters Q6 What is the material of the Multidrop Combi nL priming vessel A6 The Multidrop Combi nL priming vessel Cat no N07271 is made of blue polypropylene PP Q7 Can the Multidrop Combi nL priming vessel be autoclaved A7 Yes it can be autoclaved Q8 Can the Multidrop Combi nL reagent reservoir be autoclaved A8 Yes it can be autoclaved Q9 Where can you see the Multidrop Combi nL internal software version number A9 When the instrument is powered on the version number appears on the top of the screen during the startup animation Device information under the Options menu also provides the internal s
68. r OK Buzzer e Options 4 Select OK Accept 62 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Routine Operation Instrument options Use the Left and Right arrow keys to select whether the buzzer audible q sound is On or Off Press OK Calibration You can calibrate the dispensing valves for different liquids Calibration of the Multidrop Combi nL instrument is routinely carried out with the PC software For more information refer to the FIL Lit Software for Multidrop Combi nL User Manual Cat no N07173 For more information on calibrations refer also to Calibration on page 30 and Dispensing volume and liquid on page 39 Liquid factor standalone Liquid factor is a step where dispensing accuracy can be verified and calibration corrected at one selected volume Factor is a correction factor which can be used for example after speed pressure or liquid change where there is no calibration curve for a new speed or a new liquid First dispensing of multiple dispenses into a microplate strip is carried out according to the volume selected in the Main gt Volume and Liquid window Then a gravimetric measurement of the strip is performed After that the results are entered into the formula and a new liquid factor is calculated Refer to Accuracy check on page 64 and Factor calculation on page 66 Go to the Options menu Select the Liquid factor row Device
69. racy check 63 64 66 68 adjusting dispensing height 14 15 38 40 48 49 105 adjusting dispensing offset 35 38 48 49 50 advantages of using Multidrop Combi nL 15 air pressure 30 autoclaving 73 83 84 91 back flushing 14 15 33 68 73 77 105 buzzer 62 63 C calibration 30 35 39 63 66 67 68 102 105 accuracy check 63 64 66 68 factor calculation 63 64 66 liquid factor 15 39 63 64 66 67 68 105 clamp 25 27 cleaning a clogged dispensing valve 71 76 79 cleaning a leaking dispensing valve 31 71 76 77 79 cleaning the dispensing valves 31 71 76 77 79 cleaning the plate carrier 71 83 cleaning the priming vessel 71 83 cleaning the reagent reservoir 71 79 82 cleaning the tubings 71 73 79 column selection 41 44 column row selection column selection 41 Thermo Fisher Scientific full plate 40 row selection 35 40 43 selected wells 44 computer interface 35 60 93 control panel 32 91 100 current protocol 105 D dead volume 91 decontamination 69 72 83 84 85 86 88 89 103 105 procedure 84 default 38 39 40 45 46 47 48 49 50 51 58 64 65 67 protocols 58 values 38 67 deleting a protocol 57 97 density 46 66 105 device information 35 59 96 direction 50 51 dispensing 35 51 52 disinfecting the liquid path 76 86 dispensing 35 accuracy 91 direction 35 51 52 height 14 15 38 40 48 49 105 liquid 35 39
70. rds Figure 5 29 gt Figure 5 29 Pulling the dispensing valve downwards 5 Replace the dispensing valve with a new one Figure 5 30 80 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Maintenance Regular and preventive maintenance Replacing a tubing set Thermo Fisher Scientific and a manifold Figure 5 30 Replacing the dispensing valve 6 Fasten the valve screw 7 Reattach the dispensing valve tubing Figure 5 26 8 Finally prime with distilled and filtered laboratory grade water to ensure the proper functioning of the dispensing valve 9 Check the valve correction factor marked on the inspection sheet enclosed with the spare valve and use FILLit Software for Multidrop Combi nL to set the appropriate value in the valve correction menu Open FILLit Software and select Instrument gt Valve correction Enter the given value in the respective valve channel position and press Update to save the value See the details of FILLit Software in the FILLit Software for Multidrop Combi nL User Manual Cat no N07173 provided with the instrument Note Check that your instrument has the embedded software version 1 00 41 or greater to successfully utilize the valve correction factors A Note The instrument does not have to be recalibrated when you change a dispensing valve because the valve performance is consistent A To replace a tubing set and a manifold Figure 5 31
71. re colored black On the other hand the unavailable and uncalibrated speeds are colored white The available range is 1 to 5 To use unavailable speeds the liquid first has to be calibrated using the unavailable speed Press OK The optimal dispensing speeds for different liquids are factory set For example the optimal dispensing speed for water is 3 If a higher density solution is dispensed it requires a greater speed to dispense the corresponding volume You can decrease the speed for example to reduce foaming 46 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Routine Operation Protocol settings The relationship between the dispensing speed and pressure is presented in Table 4 4 Table 4 4 Relationship between dispensing speed and pressure Dispensing speed Pressure speed 1 3 psi speed 2 4 psi speed 3 5 psi speed 4 6 psi speed 5 7 psi Shaking The Shake function allows you to shake the plate immediately after dispensing it without any additional selection steps By default the shaking is turned OFF Go to the Settings menu Select the Shaking row Settings Options Mr i F r T Predispense 100nl Cc Direction OK Shaking Press OK Options lTime 4 gt Speed OK Accept Use the Up and Down arrow keys to select the duration of the shake You can set the time in increments of 1 s The maximum shaking period is 120 s The d
72. reservoir with water or detergent when necessary 3 Finally rinse with distilled and filtered laboratory grade water 4 Dry the reagent reservoir before use 5 Reconnect the reagent reservoir The standard 250 ml reagent reservoir Cat no N07489 is made of clear polypropylene PP and can be autoclaved The autoclaving conditions of the reagent reservoir are 1 bar pressure at 121 C for 20 minutes A bigger autoclavable 1000 ml reagent reservoir Cat no NO7490 with a longer tubing assembly is also available How to clean the Clean the plate carrier Figure 2 2 when it gets dirty and if you spill plate carrier liquids on it during use To clean the plate carrier follow the instructions below tissue paper soaked in a mild detergent solution e g dishwashing liquid soap solution or 70 ethanol Wipe up spills immediately Do not use formaldehyde or strong alkaline solutions A Caution Clean the plate carrier surface if needed using a soft cloth or If you have spilled infectious agents on the plate carrier decontaminate according to Decontamination procedure on page 84 A Warning Ensure that the bottom of each microplate is dry Fluid on the bottom of a microplate may present a contamination hazard Use proper laboratory practices when handling any hazardous materials A How to clean the Clean the priming vessel Figure 5 32 once it gets dirty during use priming vessel To clean the priming vess
73. s now fastened Figure 5 35 Thermo Fisher Scientific Maintenance Packing for service A if Screw 2 Screw 1 Figure 5 34 Refitting the transport lock Screw 1 Screw 2 Figure 5 35 Transport lock fastened Packing for To pack for service follow the guidelines presented below service Caution It is important that the instrument is thoroughly decontaminated before it is removed from the laboratory or any servicing is performed on it A Prior to shipping the instrument for service remember to specify the fault when you are in touch with your local Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 87 Maintenance Service contracts This procedure assumes that the original dispenser packaging materials are being used When you ship the instrument for service remember to e Empty the tubings of the dispenser and remove any loose items from the plate carrier for example plates and priming vessels before decontamination e Switch the power off and disconnect the mains power supply cable e Decontaminate the instrument beforehand e Install the transport lock of the instrument Refer to How to refit the transport lock on page 86 e Replace the red luer plugs to avoid particles from entering the pressure vacuum system unnecessarily Figure 3 9 e Replace the red ma
74. sed in automated systems Warning If other reagent reservoirs than recommended by Thermo Fisher Scientific are used we are not liable for any damages arising out of their use Never connect glass bottles if they are not intended for high pressure vacuum usage A In nanodispensing however there might be a shortage of reagent A separate reagent vessel or tube filled with the liquid to be dispensed can be inserted into the reagent reservoir Standard laboratory test tubes 50 ml and 15 ml can be placed inside the reagent reservoir as such but you will need a tube holder to keep smaller test tubes upright You can remove the reagent filter if you use separate small reagent tubes The solution is filtered beforehand with a suitable filter The 43 pm white reagent filter Cat no N07493 can be replaced by pulling the filter away from the end of the tube Figure 4 18 In some maintenance situations for example when cleaning a leaking or clogged dispensing valve it is easier to remove both the filter and the connective tube from the cap of the reagent reservoir Refer to How to clean a leaking dispensing valve on page 77 Thermo Scientific Multidrop Combi nL User Manual 31 Routine Operation Control panel Control panel Keyboard Arrow keys and OK button Keys AV lt gt a FL Figure 4 18 Removing the reagent filter This section describes the Multidrop Combi nL control panel and internal software
75. selected columns or rows Go to the Main menu Select the Select columns and rows row Select OK Accept If you want to dispense into all the wells of the plate select Full plate if it is not already shown on the screen If you want to dispense only into some of the wells select Select columns or Select rows using the Down arrow key Thermo Scientific Multidrop Combi nL User Manual 40 Column selection Ay lt gt Vea Setting the column volume Thermo Fisher Scientific Routine Operation Dispensing parameters Column number EMPTY Cir all 4 Move OK Fill Clear Use the arrow keys to move from one column to the next Press the OK button every time to select a column The selected column changes from red filled to uncolored empty Use the OK button to toggle between a filled or empty column Use the Down arrow key and select Accept and press OK after selecting all the columns you wish OK Accept STOP Escape You can press the STOP button to exit the column selection without making any changes The dispensing volume selected in the Volume and Liquid window see Dispensing volume and liquid on page 39 is shown in the bottom left box You can adjust the dispensing volume for each individual column Thermo Scientific Multidrop Combi nL User Manual 41 Routine Operation Dispensing parameters AV Volume value box EMPTY Clr all 4 gt Move OK Fill Clear Press the
76. sher Scientific Appendix A Certificate of Decontamination Name Address Tel Fax Instrument Serial no A I confirm that the returned items have not been contaminated by body fluids toxic carcinogenic or radioactive materials or any other hazardous materials B I confirm that the returned items have been decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological e Radioactive Specific information about contaminants Decontamination procedure Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by the undersigned to be free of radioactive contamination Date and place Signature Name block capitals PHOTOCOPIABLE Please include decontaminating solution used Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 103 Certificate of Decontamination 104 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Glossary back flush empty The operation of discharging the contents of the tubing of fluid calibration Performed with FILLit Software to obtain a series of calibration points for a liquid The dispensed liquids may have significantly different properties which affect the valve open time needed to achieve the same volume CE Mar
77. specifications are presented in Table 6 7 Thermo Fisher Scientific Table 6 7 General specifications General specifications Overall dimensions Weight total Mains power supply Power consumption Heat dissipation Fuses Operating conditions indoor use only Transportation conditions Storage conditions Autoclaving conditions of the priming vessel and reagent reservoir Control panel Interface Plate types Dispensing volume range Dead volume Dispensing speed Dispensing accuracy Dispensing precision CV 355 mm 14 in W x 375 mm 14 8 in D x 220 mm 8 6 in H 9 6 kg 21 2 Ibs 100 240 Vac 50 60 Hz nominal operating range 90 256 Vac 100 VA max 341 BTU max None 10 C to 40 C 40 C to 70 C packed in transport packaging 25 C to 50 C packed in transport packaging Pressure 1 bar Temperature 121 C Time 20 min Keys arrow keys OK START STOP PRIME EMPTY SHAKE PRESSURE TIP WASH Serial RS 232C USB 1 1 2 0 compatible 96 384 and 1536 well plates FILLit Software in 1 nl increments Standalone instrument 50 999 nl in 1 nl increments 1 00 9 99 ul in 10 nl increments 10 0 50 0 ul in 100 nl increments lt 1 2 ml 384 well plate 50 nl in 6 seconds 1 ul in 8 seconds 1536 well plate 50 nl in 21 seconds 1 ul in 27 seconds LES gt 1 ul 2 50 nl CV lt 10 0 5 ul CV lt 5 1 10 ul CV lt 4 gt 10ul CV lt 2 Reagent recovery
78. ssssesesssssesesesseressresseesse 15 Functional Description ccssssssssseessssseseeseseeaeseeeseransesetansesesanaeees 17 Insicument ayo ee a a a 17 BE IVE VIC Wy ooren e sass T E R E 17 Dack VI aa A att aa T i cn tad 18 LES CLL L111 Baap Hee te a Re eel eS SP eee ET oD 19 Delivery ehee eran renee ne creer N rr earner ene ere eer 19 elt LIC E pesan n E E E E A 19 Uppa Ki sne mnestiae oecicinod tip iltn tinny vane menaionens deter ecaotetes 19 Reg iremeni Sesan nian r e o a a chanson NE 20 Precautions and MI ON saa a E 20 Liatptall ato setup aa a vel caasoveesanianstuaseorten eae 21 Releasing the transport lock i otarecevertssnucnaqestereiasieeetenendasenemusives 21 Installing the priming Vessel stig ag neal esate a e 22 Installing the pressure vacuum system reSe rvOil eeeeeeeeseeeeeeeee 29 Cpe rational Che che ict ian tastwneyeeanccas Giana amr miagiel a ueniaeeaniawes 2 Routine Operation sscsrnieninna 29 Dis Pens te val vesera hacedderaeadbeateteaatchodeteeenenae nodes 29 E hare eer mee eee mre ro ere ee ene reer grec ee ete yee iene ee rey 30 Ke CMU LESELy OUI aa T A TEA S 31 Control pane leeren e en oeaeeeteaanieea tees 32 IS CV DOAUG penie E a S 32 ICY EEA E he isa ater E E A Ris nana A aca 32 DA Ay APE O A I E AE O N 33 Navic ai O 34 Dispensing scosseaacciaiessenieitatenmiareab O E TE 36 Thermo Scientific Multidrop Combi nL User Manual 7 Contents Using PILE RC SONNA a T 37 Using MiLeriial SOM Wale ann E
79. st shown as three dots at the displayed name The protocol name is automatically capitalized A Protocol name W 1 acd AE c DIE F elH n uK 4p Move OK Add STOP Escape To remove characters use the Backspace button Use the Down arrow key and if needed the Left and Right arrow keys to select the Backspace button and then the OK button to remove the character s v4b Move OK Save STOP Escape Use the Down arrow key to select the Save button and then press the OK button to save the edited protocol name Thermo Scientific Multidrop Combi nL User Manual 55 Routine Operation Running a protocol The sandglass window will appear while the Save action is in progress v4b Move OK Save STOP Escape The protocol name is now shown on the Select and Save Protocol row 384 low prof 10mm 50nl C Full plate OK Select and Save Protocol You can create a maximum of 100 protocols including the factory defined protocols When you save the protocol all the following parameters are saved mert Sons e Settings 56 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Routine Operation Running a protocol Select protocol To select an existing protocol for a particular plate type first select the p gp p plate typ plate type see Plate type on page 37 and then select the Select and ab cE _ nis row Opens protocols for 384 well plates
80. strument 7 Close the bag firmly and leave the instrument in the bag for at least 24 hours Thermo Scientific Multidrop Combi nL User Manual 85 Maintenance How to refit the transport lock 8 Remove the instrument from the bag 9 Clean the instrument using a mild detergent e g dishwashing liquid 10 Remove any stains using 70 ethanol 11 Disinfect the liquid path of the instrument with isopropanol 10 bleach for example sodium hypochlorite or 70 ethanol Last flush with distilled and filtered laboratory grade water 12 Wash and disinfect the reagent reservoir 13 The priming vessel and the reagent reservoir can be autoclaved All the tubings can be autoclaved in the same bag with the reagent reservoir 14 After performing this decontamination procedure enclose a signed How to refit the transport lock and dated Certificate of Decontamination both inside the transport package and attached to the outside of the package see Appendix A Certificate of Decontamination Remove the red transport lock from the back panel of the instrument Figure 5 33 Figure 5 33 Transport lock storage location 2 Adjust the dispensing valve lifting mechanism height and plate 86 Thermo Scientific Multidrop Combi nL User Manual carrier position to fasten screw 2 of the transport lock Figure 5 35 Then fasten screw 1 and the transport lock tag to the dispensing valve head The transport lock i
81. t with the valves cf Figure 5 24 Press the EMPTY button for several minutes Thermo Fisher Scientific Thermo Fisher Scientific Maintenance Regular and preventive maintenance Detaching the dispensing valve tube ends is only recommended if the basic cleaning procedures tip washing and emptying are not considered sufficient 2 Detach the flexible dispensing valve tube ends Figure 5 25 7 Fd 7 Figure 5 25 Detaching a dispensing valve tube end 3 Place the tube ends into a waste container for example a decanter 4 Add water or detergent for example 1 Micro 90 into the reagent reservoir 5 Press the PRIME button to raise the pressure This action leads to fluid dripping into the waste container from the valve tube ends 6 When you have rinsed enough release the pressure by pressing the PRESSURE button or by pushing the protective cover in 7 Repeat Steps 3 to 5 with the fluids you want to use if necessary 8 Prime with distilled and filtered laboratory grade water 9 Reattach the 43 pm reagent filter into the reagent reservoir replace if necessary by pushing the reagent filter into the cap tube end Thermo Scientific Multidrop Combi nL User Manual 75 Maintenance Regular and preventive maintenance Disinfecting the liquid path Routine maintenance of the dispensing valves tips Daily maintenance 10 Prime with distilled and filtered laboratory grade water 11 Reconn
82. the Multidrop Combi nL internal software An example of a warning message is shown below Dispensing volume exceeds well volume OK Continue anyway STOP Cancel Press OK to acknowledge the warning and then reselect the dispensing volume This instrument is designed to provide full user protection When correctly installed operated and maintained the instrument will present no hazards to the user The following recommendations are given to ascertain added user safety Ensure that the mains supply cable supplied with the unit is always used The mains plug should only be inserted into a socket outlet provided with a protective ground contact Thermo Scientific Multidrop Combi nL User Manual 99 Troubleshooting Guide Hazards A Mechanical Environmental Defects and abnormal stresses Warning There are no user replaceable parts inside the instrument The user should not remove the cover from the Multidrop Combi nL A The same precautions applicable when using any electrical equipment should be observed with this instrument Do not touch switches or electrical outlets with wet hands Switch the instrument off before disconnecting it from the mains supply The dispensing valve head is a free moving mechanical device controlled by the instrument and not directly by the operator It is designed for hands off operation and should be used as such Never reach into the work space while the instrument is performin
83. tly different on your system depending on the firmware version No liability for consequential damages Thermo Fisher Scientific shall not be liable for any indirect or consequential damages whatsoever arising out of the use or inability to use this product Power failure The system requires uninterrupted power supply in order to operate correctly Thermo Fisher Scientific has no responsibility whatsoever for system malfunctions arising from power failures Intended users How to use this user manual For more information Thermo Fisher Scientific About This User Manual This user manual is written for the actual end user for example research scientist or laboratory technician and provides information on the Thermo Scientific Multidrop Combi nL including the installation and operating instructions This user manual is designed to give you the information to e Review safety precautions e Set up the Multidrop Combi nL e Use the Multidrop Combi nL internal software e Carry out dispensing procedures e Perform basic cleaning and maintenance procedures e Troubleshoot the instrument performance This user manual also describes all the features and specifications of the Multidrop Combi nL instrument as well as ordering information Read the manual in its entirety before operating the instrument Keep the user manual for future reference The user manual is an important part of the instrument and should be readily available
84. to the home position and the dispensing valve lifting mechanism to the up position e The clamp Figure 3 14 opens Thermo Scientific Multidrop Combi nL User Manual 25 Installation Operational check h MULTIDROP COMBI ni i N a Light indicator L r w lt Clamp mm on Priming vessel with a crest in the middle where the priming liquid is aimed at to avoid splashing and a drain gt d fe y J a i Safety grooves designed to slope and prevent fluid from entering the instrument Figure 3 14 Priming vessel inserted into place 2 Ensure that the priming vessel is inserted correctly into its slot Figure 3 14 3 Pull the protective cover over the dispensing valve head 4 Press PRESSURE PRESSURE e The pump starts and the instrument raises the pressure e The light indicator blinks while the instrument regulates the pressure e The light is stable once the pressure has been reached hazardous situation flood or spillage Caution Unfastening the tubing while the pressure is on can cause a e Check that there is always enough liquid in the reagent reservoir and ensure that the filter is below the liquid level e Check after each dispensing that the priming vessel is not filled up if the tube drain is not used A 5 Press PRIME PRIME e The lifting mechanism is lowered and the dispensing valves are opened as long as you press PRIME
85. ture use A Note You can also select more plates or define new plates with FILLit Software and download them to the instrument if needed A 38 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Dispensing volume Thermo Fisher Scientific Routine Operation Dispensing parameters Go to the Main menu Select the Volume and Liquid row shown Range BUnl 50 0pl Factor 1 000 Select the dispensing volume Volume using the Up and Down arrow keys You can speed up the selection by holding down the arrow key continuously Then select Liquid Calibration using the Left and Right arrow keys The different liquids set are e Water e 1 BSA bovine serum albumin e 30 glycerol e DMSO dimethyl sulfoxide e Methanol If you have a different liquid than the five liquids mentioned above you can calibrate the new liquid with FILLit Software or use the liquid factor to calibrate the set volume The optimal speed dispensing volume range and the liquid factor for the dispensed liquid are displayed in the Volume and Liquid window Thermo Scientific Multidrop Combi nL User Manual 39 Column Row selection Full plate y Thermo Fisher Scientific The default maximum dispensing volumes and dispensing heights for different plate types are given in Table 4 3 Accept the selections using the OK button You can dispense either into the whole plate or only into certain
86. u 384 low prof 10mm C Full plate abc Default protocol OK Plate type Press OK Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 37 Routine Operation Dispensing parameters 96 standard 15mm 96 low DW 22mm 364 low vol 7 5mm 364 standard 15mm Select OK Accept Select the plate type using the Up and Down arrow keys Press OK to accept the selection The available plate types and the default parameter values are given in Table 4 3 Each plate type has a selected default maximum dispensing volume and dispensing height which is automatically set by the instrument Table 4 3 Default parameter values for different plate types Plate type Max Dispensing dispensing height mm volume pl 96 well standard 15 mm 500 16 96 well low DW deep well 22 mm 600 23 384 well low volume 7 5 mm 25 8 5 384 well low profile 10 mm 70 11 384 well standard 15 mm 130 16 384 well DW 22 mm 200 23 1536 well low volume 5 mm 2 6 1536 well standard 10 5 mm 13 11 5 You can dispense without warning to the maximum dispensing volume but once the maximum is exceeded a warning appears and you have to press OK to continue dispensing The default dispensing height is 1 mm above the selected plate Note If the default plate values do not agree with yours change the dispensing offset and height values in the Settings menu You can save the plate values for fu
87. ue in the calibration curve Refer to Accuracy check on page 64 66 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Thermo Fisher Scientific Routine Operation Instrument options E Options Factor START Dispense OK Accept 2 The value of the factor can be changed with the Up and Down arrows in the Liquid factor window The default value is 1 000 and when changed the volume dispensed will be changed in proportion to the factor In this case the instrument dispenses 0 984 times liquid compared to the value in the calibration curve 3 Save the protocol Note The factor affects the whole calibration curve throughout the volume range and the volumes are changed respectively in the same proportion as the check volume Figure 4 22 The Liquid factor is however protocol sensitive and must be saved with the protocol The liquid factor does not affect the calibration curve itself A MULTIDROP COMBI nL LIQUID FACTOR e NEW LIQUID Dispensed with water calibration a NEW LIQUID Water calibration amp Liquid Factor 200 nl A NEW LIQUID Water Calibration amp Liquid Factor 10000 nl ACCURACY Set Volume nl Figure 4 22 Calibration curve vs factor and volume changes Thermo Scientific Multidrop Combi nL User Manual 67 Routine Operation screen saver Screen saver Shutdown Note If only the speed which relates to the pressure has b
88. ump 97 lifting mechanism 97 reagent filter 31 32 53 73 74 75 77 78 82 95 101 reagent reservoir 13 23 24 25 26 30 31 36 53 68 71 73 75 77 78 79 82 83 85 86 91 95 96 101 refitting the transport lock 72 86 88 releasing the transport lock 21 remote control 93 105 remote mode 93 105 replacing a dispensing valve 71 79 replacing a tubing set and manifold 72 81 rotor cover 97 running a protocol 53 S saving a protocol 35 38 45 54 55 56 57 62 67 screen saver 68 selecting a protocol 57 selecting columns 40 41 44 selecting columns and rows 44 selecting rows 35 40 43 44 selecting wells 44 serial cable 101 serial number 4 59 88 96 service 69 71 72 85 87 88 97 101 contracts 72 88 setting the column volume 41 setting the row volume 43 shaking 35 47 shutdown 68 72 specifications 3 88 89 91 92 speed 15 30 39 46 47 48 63 65 68 95 105 startup 33 35 36 53 58 61 96 Thermo Fisher Scientific protocol 35 58 61 stopping the protocol 32 symbols and markings 4 5 6 T technical specifications 91 tip 33 75 77 91 105 tip washing 33 75 77 91 105 transport 19 20 21 22 72 86 87 88 91 100 lock 21 22 72 86 87 88 package 19 86 troubleshooting 27 97 tubing 6 26 31 36 72 73 77 78 79 81 82 83 95 101 105 U unpacking 19 USB 14 60 91 93 101 105 using
89. ur 4 Table 4 5 Speed settings vs distance movement and frequency 48 Table 5 6 Maintenance Checklist cece ccececeeceseeeccessseeereseesterenneen 71 Table 6 7 General specifications oo cee ceecceeceseesesessesereseeereneeeeeres 91 Table 8 8 Error Messages reported cee cccccecceceecseesseccessecseeeesteatseeeteees 97 Table 9 9 Instrument catalog nUmMber seeeerrreeee 101 Table 9 10 Codes for accessories 101 Thermo Scientific Multidrop Combi nL User Manual 11 Tables 12 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Thermo Fisher Scientific Chapter 1 Introduction to the Multidrop Combi nL The Multidrop Combi nL Figure 1 1 is an automatic programmable eight valve microplate bulk reagent dispenser for nanoliter to microliter volume dispensing It covers the volume range 50 nl to 50 pl It has a pressure vacuum based dispensing system for rapid and continuous dispensing of liquids into 96 384 and 1536 well plates It can dispense 50 nl into the entire 384 well plate in 6 seconds or 50 nl into the entire 1536 well plate in 21 seconds The Multidrop Combi is lightweight transportable and compact on a laboratory bench Figure 1 1 Multidrop Combi nL microplate dispenser The instrument model available is e 5840400 Multidrop Combi nL 100 240 V 50 60 Hz Each Multidrop Combi nL comes with a pressure vacuum system reservoir that
90. ve for example persistent air bubbles from the hosing and microsolenoid dispensing valves use tip washing The tip washing is started by pressing the TIP WASH button A sequence of operations is carried out to remove the debris or air bubbles The liquid comes in pulses and one liquid channel at a time to maintain a maximum flow through the dispensing valve Emptying To routinely clean the tubes and microsolenoid dispensing valves use emptying The emptying is started by pressing the EMPTY button The EMPTY button is also used for emptying the remaining liquid from the tubings and dispensing valves backflush option Periodic maintenance Periodic maintenance constitutes for example cleaning a leaking or clogged dispensing valve replacing a dispensing valve or replacing a tubing set and a manifold The procedures are all explained in detail below How to clean a leaking Foreign particles can cause leakage of a dispensing valve tip dispensing valve Figure 4 16 and Figure 5 30 A dispensing valve leaks when a droplet forms on the end of the dispensing valve while the pressure is on To clean a leaking dispensing valve 1 Press the TIP WASH button several times The liquid comes in pulses and one channel at a time to maintain a maximum flow through the dispensing valve 2 However if the dispensing valve is still leaking try the following backflush procedure e Empty the reagent reservoir or fit an empty reagent reservoir an
91. ves and laboratory coats according to good laboratory practice Ensure that the working area is well ventilated Never spill fluids in or on the equipment Thermo Fisher Scientific Installation Installation setups Installation This section describes the installation setups that you must carry out before operating or relocating the instrument setups j a Warning Ensure that the mains switch Figure 2 4 at the left of the back panel is in the OFF position Also ensure that the mains supply cable is disconnected A Releasing the The instrument has a transport lock Figure 3 5 transport lock Make sure the red transport lock is released before operating the instrument Figure 3 5 Transport lock and transport lock tag present 1 Unscrew the two screws marked 1 and 2 of the transport lock with the hexagonal screwdriver supplied Figure 3 6 so that the track mechanism is loosened Screw 1 Screw 2 Figure 3 6 Removing the transport lock Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 21 Installation Installation setups 2 Remove the screws the transport lock piece and the transport lock tag from the screw marked 1 Figure 3 6 Keep the tag for future relocation or transportation of the instrument The transport lock is now released 3 After that fasten the red transport lock piece to the back panel of the dispenser with the same screws for storage Figure 3
92. ways after using the instrument Warning Before a prolonged standby run the tubings dry to avoid microbial growth from developing A If there is reason to suspect that particles have entered the tubings clean the tubings Note To ensure trouble free dispensing do not touch the dispensing valves or the tubing inlet Thermo Fisher Scientific assumes no liability for the use of third party dispensing valves A To clean the tubings 1 First carry out the following backflush procedure e Empty the reagent reservoir or fit an empty reagent reservoir and remove the reagent filter at the bottom of the reagent reservoir by pulling it off by the end of the cap tube Figure 5 23 Thermo Scientific Multidrop Combi nL User Manual 73 Maintenance Regular and preventive maintenance Figure 5 23 Removing the reagent filter Place a disposable reagent basin Cat no 9510027 or a 96 well plate filled with detergent for example filtered 1 Micro 90 International Products Corporation or equivalent under the dispensing valves so that the liquid is in contact with the valves Figure 5 24 Figure 5 24 Cleaning the tubings using a reagent basin 74 Thermo Scientific Multidrop Combi nL User Manual Press the EMPTY button for several minutes Place another disposable reagent basin or a 96 well plate filled with distilled and filtered laboratory grade water under the dispensing valves so that the liquid is in contac

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