Model 44 Syringe Pump Series User`s Manual
Contents
1. l Refill lt _ Silicon tubing 1 4 inch O D 1 8 inch I D Infuse gt Syringe Refill Ey d N2. Catalog No Product 55 1146 Low pressure Auto Fill Accessory 55 1145 High Pressure Auto Fill Accessory Additional Accessories Catalog No Product 55 4144 Foot Switch for 44 Pump 55 7760 Pump Chain Cable for connecting two or more 44 Pumps 55 4145 RS 232C Connector Cable for IBM and compatible personal computers 55 4146 RS 232C Connector Cable for other than IBM and compatible personal computers z Harvard Apparatus Syringe Pump 4400 Appendix J Custom Applications The Harvard 44 Programmable Syringe Pump lends itself to a multitude of OEM industrial applications For all types of custom pumping or pilot plant applications the pump s program ming capabilities can be enhanced Please contact the Harvard Development Group if we can be of help Pump 44 Variations 98 0759 98 0782 98 0774 Multi Syringe Pump with up to 10 Syringes 20 ml 5 pl Multi Syringe Pump with up to 6 Syringes 30 ml 140 ml Higher Pressure Single Syringe Pump 250 lbs of Force Remote Single Syringe Pump 250 Ibs of Force Harvard Apparatus Syringe Pump 4400 Appendix K Auto Fill Valves Two models are available 55 1146 Supplied with 1 8 inch I D 1 4 inch O D silastic tubing and a connector to the syringe 55 1145 Supplies with 1 4 inch O D 304 stainless steel tubing with Swagelok fittings Setting Up the Valve Set up consist of three phases Voltage selection
3. 4 Stop the pump PROGRAM PRINTOUT EQ 1 PROFILE 0 000 ml mn 2 00 01 INTERVAI FUSE EO 24 INCR 1695 INCR 00 01 INTERVAI TFUSE EPEAT EQ 3 PROFILE 0 000 ml mn 00 10 INTERVAL FUSE EQ 4 STOP 0 000 ml ite wv S 1 0 I S 0 0 I 5 3 2 0 I S 1 Programming Tutorial Contd Harvard Apparatus Syringe Pump 4400 Example 3 SEQ 75 1 a O RATE ml min i oa a EXTERNSL TRIGGERS TIME gt Here a series of dispenses are programmed Each dispense is started by a trigger such as pressing the RUN STOP key or pressing an attached foot switch Seven dispenses are programmed three of 15 ml at 35 ml mn two of 25 ml at 65 ml mn and two of 17 ml at 45 ml mn The pump s display will show TRIGGER when it is waiting for a run trigger and the Run LED will be off This is a Strategy 1 dispense A time interval of 0 is specified when entering a Strategy 1 dispense Since the total volume to be dispensed is 129 ml and the syringe volume is 50 ml the Auto Fill feature would be very useful with this program PROGRAM PRINTOUT EQ 1 DISPENSE 5 000 ml mn 5 000 ml REPEAT IFUSE FQ 2 DISPENSE 5 000 ml mn 5 000 ml REPEAT J EQ 3 DISPENSE 5 000 ml mn 7 000 ml REPEAT NFUSE EQ 4 S 3 1 3 I S 6 2 2 T 3 4 1 2s T S STOP Harvard Apparatus Syringe Pump 4400 Programming Tutorial Contd Example 4 5 RE
4. Command Formats amp their Meaning sceeeeseeees 33 Pump Commands amp Responses ssssssesesseeseeeeeeeeees 34 40 Appendices A Table of Syringe Diameters neccen 41 BHFlOW Rates enen ornen ELL A eee 42 C Specifications cccecccecceeceeeeeeeeeeneeeeeeseeeeneeeeeees 43 D Pump Chain Command Summary 0006 44 45 E RS 232 Specifications ccccccceceeeeeeeeeeseeeees 46 47 F TTL Connector Specifications cecceeeeeeeees 48 G Maintenance ewen loa ead 49 H Troubleshooting 00 eeeeececeeeeeesseeeeeeeeeennneeeeeeeeaas 50 LACCOSSONGOS anna na E AEE 51 J Custom Applications Pump 44 Variations 52 K Pump 44 Auto Fill Valves 53 54 Harvard Apparatus Syringe Pump 4400 General Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency While the flow will stay calibrated the peak will vary Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty exten
5. RUN STOP key while the pump is running stops the pump and the right side of the display will indicate INTERRUPT plus a gt for infusing or a lt for refill ing This indicates that the pumping operation has been suspended and can be continued Pressing the RUN STOP key again will continue the pumping opera tion at the point that it was interrupted Changing any of the settings including the pumping direction and the run mode cancels the interrupted operation and resets the volume delivered display to 0 0000 ml The pump can also be started and stopped from a remote source 3 Harvard Apparatus Syringe Pump 4400 Program Mode Program Description A program is made up of a set of sequences Each sequence being a set of operat ing instructions for the pump to follow When the pump is started in the PRO GRAM run mode the pump will start at sequence 1 and execute the operating instructions in that sequence When the pump has completed the instructions for a sequence it will go to the next or specified sequence and execute the instructions in that sequence Presently up to nine sequences may be entered The pump con tinues this process until it either has reached a STOP operation the pump is manually or remotely stopped or the last sequence has completed A sequence consists of a sequence number indicating the order of the sequence a mode indicating what operation the sequence will be performing and the actu
6. and data entry keys Diagram 2 Set Keys HARVARD APPARATUS Target Volume Diameter Auto Fill Program Infuse peel Select Mode Toggle Data Entry Set Keys SET Allows modification of a data item in this group of keys To modify a data item press the relevant key after pressing the SET key INFUSE RATE Displays sets current infuse rate Scrolls through rate units when setting Displays current programmed rate while run ning in program mode REFILL RATE Displays sets current refill rate Scrolls through rate units when setting DIAMETER Displays sets current syringe diameter When held down during setting enters built in syringe table TARGET VOLUME Displays sets current volume mode target vol ume RS 232 Displays sets current RS 232 device s attached AUTO FILL Turns Auto Fill feature setting on off Also displays sets syringe refill volume PROGRAM Displays current sequence number of program Held down with a printer attached and the pump stopped prints a pro gram listing 5 Harvard Apparatus Syringe Pump 4400 Description of Keys Contd Toggle Keys SELECT MODE Toggles between PUMP DISPENSE PROGRAM run modes with each press of the key LED indicators display cur rent mode Pump must be stopped INFUSE REFILL Changes direction of syring
7. 00 ml hr Q 6 PROFTI 5 000 ml mn 0 0000 ml FUSE EQ 7 RESTART S 3 I SI 75 Dis I SI 0 S 7 1 I SI 3 Harvard Apparatus Syringe Pump 4400 Programming Tutorial Contd Example 7 wa E E mi mn ale Tse Rate 5 Time Before the pumping flow begins the pump sends a TTL signal to another piece of equipment for 5 seconds but first verifies that the TTL line is low by turning it off for 1 second then turns it on The pumping process itself consists of an alternation of pumping 3 ml s at 53 ml mn then pumping 5 ml s at 75 ml mn The pump uses the TTL output to sig nal to another device which sequence it is executing by lowering the TTL line before sequence 5 and raising the line before sequence 8 PROGRAM PRINTOUT j j SI 0 SI 0 SI 0 SI 0 SI 0 SI 53 3 3 ak SI 0 SI TAs De I SI 8 4 Harvard Apparatus Syringe Pump 4400 Programming Tutorial Contd Example 8 This is an example of the Pump 44 being operated from a remote location When the pump is powered on the position of the pusher block is unknown and must be homed to a known position At the syringe full position a limit switch is placed such that it is tripped by the pusher block when the syringe is full The limit switch is connected to pins 4 and 9 programmable output and the event input on the TTL connector When t
8. 110 or 220v Pump to valve elec trical connection and Valve tubing to syringe hook up 1 Voltage Selection Valves are shipped from the factory with an American line cord with three wire molded power connector and the pump set for 115 125 VAC For 220 240 VAC operation the molded power connector must be cut off and replaced with an appropriate type The line cord is color coded in International standard colors Brown High Blue Neutral Green Ground Observe the polarities when installing the new connector To adjust the valve for 220 240 volt operation an international voltage selec tor switch must be set Remove the top panel by removing eight screws four on each enc of the valve Identify the voltage selector switch located directly below Using a screw driv er turn the switch from 110 to 220 The pump is now set up for 220 volt operation 2 Pump to Valve Electrical Connection The cable fitted with a male 9 pin D sub connector must be connected to the female D sub connector on the rear of the Model 44 Syringe Pump Power can now be switched on with the illuminated power On Off switch on the front valve housing 4 Harvard Apparatus Syringe Pump 4400 Appendix K Auto Fill Valves Contd 3 Pump to Valve Tubing Connection Swagelok fitting should be finger tight plus 1 1 4 turn Tubing is 304 stainless steel 1 4 inch O D wall thickness 0 035 inch P intus oa iil gt Syringe
9. Verify pins 4 5 and 6 on the 25 pin connector are jumpered on the computer side of the cable Pump 44 Display Messages PUMP STALLED This indicates that the pusher block travel has been impeded This may be caused by the syringe plunger hitting bottom a kink in the tubing syringe plunger binding or any situation requiring more force to the head of the syringe than the pump is capable of delivering To restart the pump remove the cause of stalling and press RUN The pump will continue the interrupt ed procedure where it stopped Also note a large increase in the pumping rate could also stall the motor OUT OF RANGE A value was entered or encountered in a pump program that was beyond the pump s limits Also verify that the Infuse Rate is not 0 Note that rates must be less than 42949 Express larger rates using different units SETTINGS RESET The values stored in the non volatile memory have been lost This should never occur under normal operation REPLACE NOVRAM The non volatile memory has failed This should never occur under normal operation 5 Harvard Apparatus Syringe Pump 4400 Appendix I Accessories Auto Fill Accessory To operate the Auto Fill feature either the low pressure or high pressure valve assembly is required Each assembly contains the solenoid valve connector cable power cable and tubing and fittings to connect to the syringe Operates at 115 230 VAC 50 60 Hz via a selector switch
10. periodically with the Magnalube G R grease provided with the pump This Teflon R based grease is available either from Harvard Apparatus or Carleton Stuart Corp 13 02 44th Ave Long Island City NY 11101 Solvents of any type should never be used to clean the pump A mild detergent solution may be used to clean the key pad Fuse Replacement Make sure power cord is disconnected from main supply before replac ing fuse Remove bottom cover on pump by removing four 4 rubber feet and three 3 screws Remove two 2 nuts retaining aluminium shield Locate fuse on power supply module Remove fuse from fuse clip Use caution not to break the fuse when removing Replace fuse shield bottom cover screws and rubber feet For continued fire protection replace fuse only with specified type and rating 3AG 1 2 AMP 250V SLO BLO FUSE 1 2 AMP S B WARNING USEIN MANNER NOT SPECIFIED BY THE MANUFACTURER MAY IMPAIR THE PROTECTION PROVIDED BY THE EQUIPMENT 3 Harvard Apparatus Syringe Pump 4400 Appendix H Troubleshooting RS 232 Difficulties Verify that the baud rates and data framing parameters on all devices are the same With the pump chain a Communication timeout error on a computer is usually caused by the computer errantly handshaking on the RTS CTS and DSR lines
11. remains on the default display until the pump is turned off or SET RS 232 is entered on the keyboard indicating a change in the RS 232 configuration A pump will not respond to pump chain communication while it is in a setting mode entered when user presses the SET key The pump can still be controlled from the keyboard while it is in a pump chain Control data that is changed via RS 232 will be stored in the pump s non volatile memory After each command is received and executed the pump terminates its responses with a prompt A prompt is a string of ascii characters sent by a pump Each command sent to the pump chain is a string of ascii characters with leading zeros on numbers and all spaces optional Numbers are a maximum of five digits The following symbols are used in describing the commands Symbol Meaning Les optional ceo select one either or f digits 0 9 or a decimal point d digits 0 9 lt cr gt carriage return ascii 13 lt lf gt line feed ascii 10 lt float gt frfffe lt integer gt ddddd lt time gt d dd dd lt text gt any string of ascii characters 4 Harvard Apparatus Syringe Pump 4400 Command Formats amp Their Meaning Command Format lt cr gt pump address lt cr gt optional pump address command lt cr gt Meaning Stops all pumps All pumps on the pump chain interpret this as a stop command Request for prompt The pump with the indicated address responds wi
12. when the pump is operated Pressing the SELECT MODE key advances the LED run mode indicator Advance the run mode indicator to the desired mode either Pump Mode Volume Mode or Program Mode 1 Pump Mode The pump will continuously pump infusing or refilling until stopped While running the Infuse and Refill Rates can be changed The new rate for the relevant pumping direc tion takes effect when the ENTER key is pressed Also the pumping direction can be changed by pressing the INFUSE REFILL key 2 Harvard Apparatus Syringe Pump 4400 Operation Contd 2 Volume Mode The pump will run infusing or refilling until a specified target volume is pumped or refilled The TARGET VOLUME key is used to enter the Volume Mode pumping target Used in conjunction with Auto Fill the target volume can be greater than the volume of the syringe While running the Target Volume Infuse and Refill Rates can be changed The new rate for the relevant pumping direction takes effect when the ENTER key is pressed 3 Program Mode In the Program Mode the pump can make complex dispenses including changes in rate and target volume These complex dispenses are easily programmed from the key pad and are detailed in the Program Mode and the Programming Tutorial sections Running the Pump Pressing the RUN STOP key starts the pump The pump will operate according to the relevant data entered as interpreted by the selected run mode Pressing the
13. 0 pl 1000 0 ul 1 0 ml 2 0 ml 2 5 ml 3 0 ml 5 0 ml 10 0 ml 20 0 ml 30 0 ml 50 0 ml 100 0 ml 140 0 ml Pump 44 l min ml hr ml min min max min max min max Harvard Apparatus Syringe Pump 4400 Appendix C Pressure amp Force Specifications Rate Starting Stall Stall Force Stall Force PSI PSI Lbs Kg ml min Rate This is the rate indicated with a Harvard Apparatus 100 ml stainless steel syringe Diameter 34 9 mm cross sectional area 1 48 square inches Starting PSI This is the back pressure at which the pump would reliably start At higher pressures the pump may stall Stall PSI This is the back pressure that finally stalls the pump Stall Force This is the force coressponding to the stall PSI The same force applied to dif ferent size syringes will generate correspondingly higher or lower pressures and flow rates 4 Harvard Apparatus Syringe Pump 4400 Appendix D Pump Chain Command Summary All spaces in command are ignored Maximum 5 digits per integer float or time data Command gt lt adr gt lt cmd gt lt cr gt adr gt d d cmd gt RUN STP DEL CLD RAT lt float gt lt units gt RFR lt float gt lt units gt PGR DIA lt float gt TGT lt float gt MOD PMP VOL PGM DIR INF REF REV SEQ lt integer gt lt entry gt Description 1 or 2 digit address start pump stop interrupt pump query volume delivere
14. E SEQ 5 PROFILE 75 000 ml mn 95 000 ml mn 43 155 ml 0 00 10 INTERVAI REFILL USE SEQ 2 PROFILE Q 6 PROFILE 50 000 ml mn 000 ml mn 0 00 04 INTERVAI 0 05 INTERVAI INFUSE USE SEQ 3 DECR 7 PROFILE 4 0000 DECR 000 ml mn 0 00 01 INTERVAI 0 05 INTERVAI 12 REPEAT US INFUSE SEQ 4 INCR 8 0000 INCR 0 00 01 INTERVAL 8 REPEAT INFUSE One Hy 3 DECR 0000 DECR 00 01 INTERVAI REP IFUSE EQ 9 B NHPOURHOARHOWNH 5 Harvard Apparatus Syringe Pump 4400 Programming Tutorial Contd Example 6 Output Pin4d 0 ee oe ee cee Pin9input c E yy 1 E A E Event PLIA Y 75 Rate 5 0 Time This is an example of the Pump 44 working interactively with other laboratory equipment The pump will continuously pump at 300 ml hr until an external event a high to low transition at pin 9 possibly produced by another Pump 44 causes the pump to deliver a 15 ml bolus at 75 ml mn After delivering 5 ml of the bolus output pin 4 is set to a logic high for the duration of the bolus after which it is dropped This output pin can be attached to the timer input of another pump such as a Harvard Pump 22 to create a precise mixture during the bolus After the bolus is completed the Pump 22 would be stopped and the Pump 44 would return to delivering its background rate of 300 ml hr waiting for another external event PROGRAM PRINTOUT SEQ 1 TTL OUT OFF SEQ 2 EVENT GO TO 4 EQ 3 PU 00
15. Model 44 Syringe Pump Series User s Manual Model 44 Programmable MAI 55 1144 HARVARD APPARATUS WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any changes as they
16. OD mode Where mode is as follows Mode Description Mode Description STP stop RST restart PRO profile EVN event INC increment GOT go to DEC decrement OUT set output pin DIS dispense PMP pump PAS pause J Harvard Apparatus Syringe Pump 4400 Pump Commands and Responses Contd Set data item of program sequence n Set sequence s rate SEQ lt n gt RAT lt float gt lt units gt Rate is of format fff fff Units are one of UM UH MM MH Description ul mn ul hr ml mn ml hr Set sequence s go to sequence number SEC lt n gt GOT lt n gt Set sequence s target volume SEQ lt n gt TGT lt float gt Set sequence s time or target SEQ lt n gt INT lt time gt Set sequence s repetition number SEQ lt n gt RPT lt integer gt Set sequence s pumping direction SEQ lt n gt DIR lt INF REF gt Set sequence s output pin level SEQ lt n gt OUT lt ON OFF gt VER Request for version of pumps embedded software Response for the present version will be space space 44V2 3 Pump Chain Error Messages Error messages are in the format lt lf gt space space lt message gt lt cr gt Where lt message gt is one of the following Syntax error in a received command NA Command not applicable at this time OOR Control data is out of the operating range of the pump J Harvard Apparatus Syringe Pump 4400 Appendix A Syringe Diameter in m
17. Request to set or query syringe volume setting for auto fill Used in conjunction with Auto Fill feature See AF command Set syringe volume SYR volume Volume is of format f fff ff Units are ML If volume is accepted and valid volume will become the new syringe Auto Fill volume Volume will not be accepted if the pump is running anda Not Applicable response will be given If the volume is invalid an Out Of Range response will be given Query syringe volume SYR Response is of the following format space space f f f f f IN d Request to read the TTL logic level of the specified pin on the external 9 pin D SUB connector Valid pin numbers for input are 6 7 8 and 9 If the pin specified is valid and if the pin level is high response will be ON If the pin level is low response will be OFF i Harvard Apparatus Syringe Pump 4400 Pump Commands and Responses Contd If the pin specified is invalid An Out Of Range response will be given OUT d lt ON OFF gt Request to set the TTL logic level at the specified pin on the external 9 pin D SUB connector Valid pin number for output is 4 If the pin specified is invalid An Out Of Range response will be given Example Set pin 4 high OUT 4 ON Set pin 4 low OUT 4 OFF SEQ lt integer gt Kentry gt Request to set or query programming sequences lt integer gt is sequence num ber Default is Sequence 1 Command only applicable while
18. START RATE ml min 0 TIME min seconds This is an example of a series of periodic dispenses of varying volumes and inter vals For this application Strategy 2 dispenses are used Note that between the third and fourth dispenses is a 45 minute interval Each dispense in the first sequence is separated by a pause interval of 1 30 Since after the third dispense there already will be a 1 30 pause an additional pause of 43 30 is used to extend the pause to the desired 45 00 Sequence 5 is a RESTART command causing the series of dispenses to be continuously repeated until the pump is stopped PROGRAM PRINTOUT SEQ 1 DISPENSE 15 000 ml mn 3 5000 ml 0 01 30 INTERVAI REPEAT NFUSE EQ 2 PAUSE 43 30 INTERVAI EQ 3 DISPENSE 25 700 ml mn 6 7500 ml 0 05 00 INTERVAI 2 REPEAT INFUSE SEQ 4 DISPENSE 20 000 ml mn 4 3000 ml 0 02 30 INTERVAL 4 REPEAT INFUSE SEQ 5 RESTART i Harvard Apparatus Syringe Pump 4400 Programming Tutorial Contd Example 5 SEQ 100 4 8 RATE ml min 0 10 20 30 40 50 60 TIME seconds Here is an example of a more complex profile program Each run of the infusion has been determined to pump 43 155 ml The first sequence refills the syringe with the volume to be infused then the infusion profile is started after which the syringe is refilled and the infusion is repeated until the pump is stopped PROGRAM PRINTOUT SEQ 1 PROFIL
19. Size 8 Parity even Stop Bits 1 Auto print feature off Stable data only off Serial data frame selection Fr 7 Serial printer with print buffer Baud Rate 2400 Word Size 8 Parity none Stop Bits 2 Harvard Apparatus Syringe Pump 4400 Appendix F TTL Connector Specifications Valve Control Running output indicator User definable outputs Voc 5v A Foot Switch ho 4 Timer Refill Event TTL Connections are via a 9 pin D sub type connector All inputs are internally pulled up to a logic high Pin Assignments 1 Vss Ground reference logic low 7 Timer Input Rising edge starts pump 2 Valve control output Falling edge stops pump Low Infusing High Refilling 8 Directional control input Rising edge sets pump to infuse 3 Running Output Indicator Falling edge sets pump to refill Low Pumped stopped High Pump running 9 Event input Falling edge triggers program event 4 Programmable output Logic Low 0V 0 5V 5 Vcc logic high reference Maximum 2 mA current sink Logic High 2V 5V 6 Foot switch input Maximum 400 pA current source Falling edge starts stops pump 5 Harvard Apparatus Syringe Pump 4400 Appendix G Maintenance Pump 44 requires no special maintenance other than keeping it clean by avoiding accidental spills of pumped material The two guide rods and the lead screw should be sparingly lubricated
20. ace space fff fff Units are ML MOD PMP VOL PGM Request to set or query pumping mode Set MOD PMP Puts pump in Pump Mode MOD VOL Puts pump in Volume Mode MOD PGM Puts pump in Program Mode Command will not be accepted if the pump is running and a Not Applicable response will be given Query MOD If mode is PUMP response will be PUMP If mode is VOLUME response will be VOLUME If mode is PROGRAM response will be PRGRAM DIR INF REF REV Request to set or query pumping direction Set DIR INF sets pumping direction to infusion DIR REF sets pumping direction to refill DIR REV reverses current pumping direction J Harvard Apparatus Syringe Pump 4400 Pump Commands and Responses Contd Command will not be accepted if the pump is running in volume or program modes and a Not Applicable response will be given Query DIR If pump direction is infusion response will be INFUSE If pump direction is refill response will be REFILL AF ON OFF Request to set or query auto fill setting Set AF ON turns Auto Fill feature on Note The syringe volume is also needed for auto fill to operate See SYR command AF OFF turns Auto Fill function off Command will not be accepted if the pump is running and a Not Applicable response will be given Query AF If Auto Fill function is ON response will be ON If Auto Fill function if OFF response will be OFF SYR lt float gt
21. al data for the operation such as rates and volumes The necessary data specified for each sequence will depend on the strategy used One of two strategies may be chosen for a sequence s target Strategy 1 pumps until a target volume is reached while Strategy 2 pumps until a target time interval has lapsed When Strategy 1 is used enter a time interval of 0 00 00 then you will be prompted for the target volume See the Programming Tutorial for example pro grams Entering a Program It is advisable to plan out your program prior to entering the program into the pump Press SET then PROGRAM to begin entering a program The following is a list of possible data that can be requested when entering a pro gram and instructions on entering the data Sequence Operation Use the PROGRAM key to select the sequence s operation Operations that can be selected are Profile Increment Decrement Dispense Pump Event Go To TTL Out Pause Restart Stop When the required operation is displayed press ENTER Additional information may be requested 4 Harvard Apparatus Syringe Pump 4400 Program Mode Contd Rate Enter the rate using the INFUSE RATE key to change units Note If the rate entered is invalid an error message will not be given at the immediate time of entry An OUT OF RANGE error message will be given during the running of the pro gram Delta Rate Enter the rate increment or decrement The units of the rate ca
22. an OUT OF RANGE message is displayed when entering a rate try using a different syringe Auto Fill While setting use the AUTO FILL key to toggle between Auto Fill on and off If Auto Fill is set to on the pump will next request the volume of the syringe in milliliters The volume of the syringe is used as the refill volume of the syringe When set to on the syringe is assumed to be empty Auto Fill continuously mon itors the volume of the syringe according to the volume pumped When the pump determines that the syringe is empty the operation in progress is suspended and Auto Fill is activated The pumping direction is then reversed and the pump runs at the refill rate During the Auto Fill operation the display will indicate the vol ume in the syringe When the volume in the syringe reaches the set syringe volume Auto Fill will stop and the previous operation of the pump will resume Auto Fill continues to mon itor the volume of the syringe TTL direction output is toggled on during refill Refill Rate defaults to Infuse Rate if not set Note Auto Fill will only activate while infusing i e if the pump direction is set to Refill the pump will not stop when the syringe is full Also if the syringe plunger is manually moved the pump will lose track of the true syringe volume Selecting the Run Mode After entering any necessary operating data into the pump select the pumping mode that will be used
23. apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required 5 Harvard Apparatus Syringe Pump 4400 Table of Contents SUBJECT PAGE NO General Information sssssssssssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnn 2 3 Technical Specifications sssssssssnnsnnnnnnnnnnnnnnnnnnnnnnnn nna 4 5 Features erranen aeea AARAA CENENE EEEREN 6 Theory of Operation and Initial Setup 0000 7 Loading Syringes ccccessesseeceeeeeseeeeeeeeeeesseseeeeeeeeseneneeneee 8 User Interface ssssnnsnnsnnnnnnnnnnnnnnnnnnnnn nennen nnmnnn nnmnnn nnn 9 Description of KeyS sssssesnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnn 10 11 Entering Daia ra a rae taraa e an mi a aaeeea adanida 11 12 Operation DiAMe ter ecetet rre areena aE erani EEE S 13 Infuse Refill Rates 2 0 0 0 ccscccccccecceeeeeeeeeeeeeeeeeeeees 13 14 Auto Fillerin tet ee ee E ey 14 Selecting the RUN Mode scc 14 15 Running the PUMP cccceeesesseeeeeeeesesneeeeeeeesneaeees 15 Program Mode ccccccccccceesesceeeesseeeeeseeseesneeeeeeeeeeeseees 16 17 Description of Sequence Operations 18 20 External Control and Interfaces RS 232 Devices ccccccccccceeeceeeseesesseeeesenseaaeeeaueeeeeeeees 21 TIE DEVICES nenaon iA ELEAL E EE ieee 22 Programming Tutorial cc cccsessesseeeeseeeseeeeeeeeeeeeeees 23 31 Pump Chain Commands ssssssssssssssnnnnnnnnnnnnnnnnnnnnnnnnnnnnna 32
24. d clear volume delivered set query infusion rate set query refill rate query current program rate set query syringe diameter set query target volume set query pumping mode set query pumping direction set query program sequences AF ON OFF set query auto fill setting SYR lt float gt set query syringe refill volume IN lt d gt read TTL pin d OUT lt d gt ON OFF output TTL pin d VER query software version entry gt MOD PRO INC DEC GOT OUT EVN PMP DIS PAS RST STP RAT lt float gt lt units gt TGT lt float gt INT lt time gt RPT lt integer gt DIR INF REF OUT ON OFF GOT d units gt UM UH MM MH set query seq mode set query seq rate set query seq dispense volume set query seq interval set query seq repetition set query seq pumping direction set query TTL pin setting set query go to sequence ul min pl hr ml min ml hr 4 Harvard Apparatus Syringe Pump 4400 Appendix D Contd Command Response Output Format Description output gt lt response gt lt prompt gt response gt lt response gt lt response gt lt lf gt lt message gt lt cr gt prompt gt lt lf gt lt adr gt stopped lt lf gt lt adr gt gt infusing lt lf gt lt adr gt lt withdrawing lt lf gt lt adr gt pause interval stopped lt lf gt lt adr gt pumping interrrupted stopped lt lf gt lt adr gt dispense trigger wait stopped adr g
25. ds only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCI DENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclu sion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Holliston Massachusetts 01746 1371 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state s Harvard Apparatus Syringe Pump 4400 General Information Contd Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts a
26. e pump will stop and an error message will be displayed Error messages will be dis played with the following format SEQ n message Where n is the sequence number where an error was detected and message is the indicated error as follows INFINITE LOOP A GO TO sequence cannot specify the current sequence INVALID GO TO The target of the GO TO specified an invalid sequence number RATE UNDERFLOW A decrement sequence decremented a rate to less than or equal to 0 RATE OVERFLOW An increment sequence caused an arithmetic overflow OUT OF RANGE Specified or calculated rate is beyond the pumps capabilities with the specified syringe VOL TGT ERROR A sequence with a volume target cannot follow a sequence with a time target unless the volume delivered is zero or the pump is stopped at the start of the sequence Harvard Apparatus Syringe Pump 4400 External Control amp Interfaces External devices that can be attached to the Pump 44 are categorized into either RS 232 devices or TTL devices Pump Chains Scales and Printers are RS 232 devices all other devices are TTL devices See the appropriate appendixes for specification details on attaching devices VOLTAGE SELECT TIL PORT 2 PORT 1 RS 232 Devices On the back of the pump are two telephone jack type connectors These are the RS 232 port
27. e travel for Pump and Volume modes Pump must be stopped or in pump mode RUN STOP Starts stops interrupts pump Data Entry Keys 1 2 3 4 5 6 7 8 9 0 Used to facilitate the entering of numeric data values ENTER Stores displayed data value when setting a data item Entering Data Set Keys The yellow keys in this group are used to modify or review settings of the pumps control data To review the current setting of a control data item simply hold down the relevant key and the data setting will appear in the display In the case of the PROGRAM key the data will be sent to the printer if one is attached To modify a data setting first press then release the green SET key The display should then read SET WHAT Press the key in the SET key group whose data is to be modified The display will display the current setting Data is entered into the pump by either entering a numerical value or by scrolling through a menu of choices Always press ENTER to terminate each data request by the pump If you are to enter a numerical value the far left of the display will show ENTER followed by the units of the number to be entered Using the numerical keys on the right side of the keypad see diagram enter the new data value Up to five digit numbers are accepted including up to four decimal places Entering more than five digits will clear the previous five digits in the display Press the green ENTER key when
28. f the following format space space f f f f f f units Where units is one of the following ml mn ul mn ml hr ul hr PGR Request for the rate of pumping set during the running of a program Response is of the following format space space f f f f f f units Where units is one of the following ml mn ul mn ml hr ul hr DIA lt float gt Request to set or query syringe diameter setting Set diameter DIA diameter Diameter is of format fff fff Units are MM INFUSE and REFILL rates will be set to zero and AUTO FILL will be set to off J Harvard Apparatus Syringe Pump 4400 Pump Commands and Responses Contd If diameter is accepted and valid diameter will become the new diameter Diameter will not be accepted if the pump is running and a Not Applicable response will be given If the diameter is invalid an Out Of Range response will be given Query diameter DIA Response is of the following format space space f f f f f f Units are MM TGT lt float gt Request to set or query target volume setting Set target volume TGT volume Volume is of format f fff ff Units are ML If volume is accepted and valid volume will become the new target volume Volume will not be accepted if the pump is running and a Not Applicable response will be given If the volume is invalid an Out Of Range response will be given Query volume TGT Response is of the following format sp
29. g request will not be accept ed and a Not Applicable response will be given Otherwise no response is given RAT lt float gt lt units gt Request to set or query infusion rate setting Set infusion rate RAT rate Set infusion rate and units RAT rate units Rate is of format f ff f f f Units are one of Definition UM pl mn UH pl hr MM ml mn MH ml hr If rate is accepted and valid rate will become the new infusion rate If the rate is invalid an Out Of Range response will be given Command will not be accepted if the pump is running in the Program Mode and a Not Applicable response will be given Query infusion rate RAT Response is of the following format space space f f f f f f units Where units is one of the following ml mn ul mn ml hr ul hr i Harvard Apparatus Syringe Pump 4400 Pump Commands and Responses Contd RFR lt float gt lt units gt Request to set or query refill rate setting Set refill rate RFR rate Set refill rate and units RFR rate units Rate is of format f ff f f f Units are one of UM UH MM MH Definition pl mn pl hr ml mn ml hr If rate is accepted and valid rate will become the new refill rate If the rate is invalid an Out Of Range response will be given Command will not be accepted if the pump is running in the Program Mode and a Not Applicable response will be given Query refill rate RFR Response is o
30. he pump receives a start signal it first refills the syringe and stops when the limit switch is sensed The pump then waits for a start trigger and performs a dis pense then refills the syringe and waits again for the next start trigger PROGRAM PRINTOUT FO 5 0 00 01 EQ 6 PU 5 000 ml mn EQ 7 DISPENSE 10 000 ml mn 0 0001 ml 1 REPEAT REFILL EQ 8 PUMP 5 000 ml mn 0 000 ml FUSE EQ 9 RESTART SI 0 S 0 S OFF S 0 S 7 S 3 Harvard Apparatus Syringe Pump 4400 Pump Chain Commands The Pump Chain RS 232 interface is used to enhance the control applications of the Pump 44 This interface allows all control information including a program to be entered into the pump from an external source such as a computer In addi tion this interface allows up to 100 Pump 44 s and in certain cases other RS 232 devices to be controlled from a single RS 232 communication port on a computer Assign each pump in the pump chain a unique address from 0 to 99 This address is used to identify which pump is to receive a command and which pump is responding Configure each pump with its assigned address and the baud rate being used See External Control amp Interfaces When a pump is sent a command or a request is made for its prompt a diamond appears on the far right of the default display indicating that it is receiving RS 232 commands The diamond
31. her block are fitted with movable retaining brack ets which hold firmly the syringe barrel and plunger when refilling When loading the syringe into the pump it is necessary to adjust these brackets The pusher block is fitted with a mechanism to release the drive nut from the lead screw so that the block can be moved freely to facilitate loading the syringe Loosen the screws on the syringe block and pusher block to free the retaining brackets 2 amp 3 Diag 1 To free the pusher block from the leadscrew turn the knob on the front of the block 1 until the pin in the knob slips into the hole in the block The syringe clamp locking screw on the right side of the syringe block 4 should be loosened and the clamp rotated to the side Place the syringe barrel on the syringe holder block and move the pusher block to accommodate the plunger Make sure the syringe barrel flange and the plunger flange are held by the retaining clamps Press the retaining brackets firmly against the flanges and tighten the retaining screws Rotate the syringe clamp and press down firmly on the syringe barrel Secure in place by tightening the locking screw 4 s Harvard Apparatus Syringe Pump 4400 User Interface The user interface consists of a display area and a keypad The display consists of a 20 character alphanumeric vacuum fluorescent display and 5 LED indicators The display will show one of three types of messages default dis
32. iggered event causes an immediate continuation of the program at the specified sequence and the operation of the pump will be according to this sequence Data Specified Go to sequence number Go To Causes the program to immediately continue operation at the sequence specified Data Specified Go to sequence number Pause Pump stops for specified time then continues with next programmed sequence Current program rate set to 0 with no change in units Data Specified Time interval Pump Runs the pump continuously at the specified rate without any pumping target This mode can provide a background flow rate while waiting for an external event to trigger a new sequence specified by the EVENT operation Data Specified Rate Pumping direction Restart Immediately restart program from the first sequence Data Specified None TTL Out Programmable TTL Pin TTL output Pin 4 can be set to a HIGH or LOW level from within a program Data Specified TTL pin level Stop Stops pump and terminates program Data Specified None 5 Harvard Apparatus Syringe Pump 4400 Description of Sequence Operations Contd Program Printout If a printer is attached and the pump is stopped a program listing can be obtained by pressing the PROGRAM key for about one second PRINTING PROGRAM will be displayed while data is being sent Program Run Time Error Messages If while running a program an operation is requested that cannot be performed th
33. ir respective diameters Infuse Rate The Infuse Rate is the rate of pumping while infusing in the Pump or Volume modes Also the Infuse Rate is used as a starting rate for the program mode if one is not specified in the program regardless of pumping direction When entering the Infuse Rate the INFUSE RATE key is used to scroll through the allowable units of rate The allowable units are ml mn ml hr pl mn pl hr While running in the Pump or Volume modes the Infuse Rate can be changed If the new rate is valid it will take effect when the ENTER key is pressed The minimum and maximum rates permitted vary depending on the diameter of the syringe If an OUT OF RANGE message is displayed when entering a rate try using a different syringe for your application 3 Harvard Apparatus Syringe Pump 4400 Operation Contd Refill Rate The Refill Rate is the rate of pumping while refilling in the Pump or Volume Modes or during Auto Fill If the Refill Rate hasn t been set rate is 0 the Refill Rate will default to the Infuse Rate When entering the Refill Rate the REFILL RATE key is used to scroll through the allowable units of rate The allowable units are ml mn ml hr pl mn pl hr While running in the Pump or Volume modes the Refill Rate can be changed If the new rate is valid it will take effect when the ENTER key is pressed The minimum and maximum rates permitted vary depending on the diameter of the syringe If
34. ize Minimum Maximum Flow Rate Range Minimum Maximum Display RS 232 Interface Non Volatile Memory Enter syringe dia from table any size any make up to 50 mm dia 0 5 ul 140 ml 0 0001 pl hr 0 5 ul syringe 220 82 ml min 140 ml syringe 20 character alphanumeric vacuum fluorescent 5 LED indicators Multiplexed dual bidirectional ports Storage of all settings 4 Harvard Apparatus Syringe Pump 4400 Features Pressure and Speed Pump 44 can deliver up to 220 82 ml mm with a 140 ml syringe and is capable of pressures up to 1500 PSI with an 8 ml stainless steel syringe See Appendix C Built in Syringe Table and Custom Syringes If a non standard syringe is to be used enter the inside diameter in millimeters If a standard syringe is to be used select your syringe using the pump s built in syringe table Syringes are arranged according to manufacturer and material and then according to size The pump will look up and use the diameter for the syringe you select See Appendix A for standard syringes Infusion and Refill Rates Specify independent rates for infusing and refilling This allows a slow infusion rate then a fast refill Auto Fill Auto Fill automatically activates an externally attached solenoid and refills the syringe when it is empty This permits infusions to be virtually independent of syringe capacity Modes of Operation Pump Runs continuously infuse or refill until stopped V
35. m Stainless Steel Size Diameter 8cc 9 525 mm 20cc 19 130 50 cc 28 600 100 cc 34 900 Becton Dickinson Plastic Plasticpak Size Diameter icc 4 78 mm 3 8 66 5 12 06 10 14 50 20 19 13 30 21 70 50 60 26 70 Air Tite All Plastic Size Diameter 2 5 cc 9 60 mm 5 0 12 45 10 15 90 20 20 05 30 22 50 50 29 00 Unimetrics Series 4000 amp 5000 Size Diameter 10 ul 0 460 mm 25 0 729 50 1 031 100 1 460 250 2 300 500 3 260 1000 4 610 Terumo Size Diameter 3cc 8 95 mm 5 13 00 10 15 80 20 20 15 30 23 10 60 29 10 Sherwood Monoject Plastic Size 1 cc Size Diameter 4 65 mm 8 94 12 70 15 90 20 40 23 80 26 60 38 40 Popper e gt Sons Inc Perfektum Glass Diameter 0 25 cc3 45 mm 0 5 3 45 4 50 8 92 8 99 11 70 14 70 19 58 22 70 29 00 35 70 SGE Scientific Glass Engineering Size Diameter 25 ul 0 73 mm 50 1 03 100 1 46 250 2 30 500 3 26 1 0 ml 4 61 mm 2 5 7 28 5 10 30 10 14 57 Hamilton Microliter Series Gastight Size Diameter 5 ul 0 103 mm 1 0 1457 2 0 206 5 0 3257 10 0 460 25 0 729 50 1 031 100 1 46 250 2 3 500 3 26 1 0 ml 4 61 mm 2 5 7 28 5 10 3 10 14 57 25 23 0 50 32 6 3 Harvard Apparatus Syringe Pump 4400 Appendix B Nominal Minimum e Maximum Flow Rates Various Syringes Actual Limits Will Vary Depending on Manufacturer nominal syringe size 0 5 pl 1 0 pl 2 0 ul 5 0 pl 10 0 pl 25 0 pl 50 0 ul 100 0 ul 250
36. mming or kinking of the tubing the pump will stop and the display will read PUMP STALLED Harvard Apparatus Syringe Pump 4400 Theory of Operation Pump 44 employs a microcontroller which controls a small step angle stepping motor that drives a lead screw and half nut Microstepping techniques are employed to further reduce the step angle making flow pulsation negligible A key pad is used for entry of operating data to the pump Data can also be entered via RS 232 The microcontroller calculates the cross sectional area of the syringe selected and calibrates the flow rate and volume accumulation The numerous fea tures of the Pump 4 result from the use of microcontroller technology Initial Set Up 1 Read the manual 2 Locate the voltage selector switch on the rear panel of the pump and set it to the voltage being used If other than 110 V 60 Hz is being used the plug must be cut off and an appropriate plug installed observing the polarity of the international line cord used Brown live Blue neutral Green ground 3 Turn on main power switch located directly above the line cord on the rear panel The display will now illuminate indicating that the power connections are correct The flashing display indicates that power has just been applied 4 Load syringe see next page 3 Harvard Apparatus Syringe Pump 4400 Loading Syringes Diagram 1 Q The syringe holder and pus
37. nnot be specified Units will be the same as the units of the current pumping rate at the time the sequence is executed Target Volume Enter the delivered target volume of the sequence For increment and decrement sequences the target volume is an incremental target An incremental target is added to the delivered volume at the start of the sequence Time Interval Enter the time duration of the sequence in the form hours minutes seconds If sequence Strategy 1 is used enter 0 00 00 for the time target The maximum time interval is 9 99 99 Number of Repetitions Enter the number of times the sequence is to be repeated The repetition number can be from 1 to 99 999 Pumping Direction Each sequence that specifies a pumping operation also specifies a pumping direc tion Use the INFUSE REFILL key to change the pumping direction Pin Level Select either HI or LOW for the logic level of the programmable output pin 4 Use the PROGRAM key to change the setting Go to sequence number Enter the destination sequence to continue operation of the program Valid sequence numbers are 1 to 9 3 Harvard Apparatus Syringe Pump 4400 Description of Sequence Operations Profile Runs at specified rate until target volume is pumped or a time interval has elapsed Travel direction is as specified Data Specified Strategy 1 Rate Strategy 2 Rate Target volume Time interval Pumping direction Pumping direction Incr Increment
38. olume Runs until a specified volume has been pumped or refilled Program Pump operates according to a specified sequence of instructions Note All modes interact with Auto Fill feature External Connections TIL Allows pump operations to be synchronized with external devices or by a person at a distance from the pump Direction of pump travel can be set viaa TTL pin Also a TTL pin is used to control an external valve for refilling Additional TTL pins are available for general use RS 232 Multiple pumps can be daisy chained together and remotely con trolled from a computer or any device communicating via RS 232 A scale can be attached enabling the pump to infuse by weight instead of by volume A printer can be attached to record final volumes or weights whenever the pump stops In addition the program entered for the program mode can be listed on an attached printer Nonvolatile Memory All operational data entered into the pump from the keypad or from a computer will be stored including the program On power up the display will blink until the pump receives its first command and all settings from when it was powered down will be recalled If in an unlikely event a failure to properly recall the data occurs the settings will be reset and the display will read SETTINGS RESET Stall Detection An optical detector is used to verify expected movement of the motor If the motor is prevented from turning due to ja
39. on of pump travel When the output is a logical high the pump is set to refill A logical low indicates infuse 4 Harvard Apparatus Syringe Pump 4400 Programming Tutorial In the following examples the diameter is 26 7 mm and the infuse rate is 50 ml mn To run a program after entering it select Program Mode using the SELECT MODE key and press the RUN STOP key Example 1 N ol RATE ml min gi l 25 0 5 10 15 VOLUME ml The following program will instruct the pump to infuse according to the above graph The program instructs the pump to infuse 10 ml at 75 000 ml mn then infuse another 5 ml at 25 ml mn then stop for a total of three sequences Since this graph is Rate vs Volume Strategy 1 will be used when entering the program Harvard Apparatus Syringe Pump 4400 SEQUENCE 1 Key Presses SET PROGRAM PROGRAM ENTER 75 INFUSE RATE ENTER 0 ENTER 10 ENTER INFUSE REFILL ENTER SEQUENCE 2 Key Presses PROGRAM ENTER 25 INFUSE RATE ENTER 0 ENTER 5 ENTER INFUSE REFILL ENTER SEQUENCE 3 Key Presses PROGRAM ENTER Programming Tutorial Contd Explanation Allows program entry Press until PROFILE selected Enters selection Enter rate of 75 000 ml mn Press until units are ml mn Enters rate Enter 0 for the time this indicates Strategy 1 10 ml is the first target volume Toggles direction to infuse Enters sequence s pumping direction Explanation Press
40. play setting display or an informational message The default display is divided into three sections The first consists of the delivered volume or time interval remaining in an operation expressed with 5 digits plus a decimal point Next is the units of the previous number expressed as ml for mil liliters g for grams if a scale is attached or time for a time interval Third is the pumping direction either INFUSE or REFILL or the current operating state of the pump A diamond symbol in the last character of the display indicates pump chain communication has been received Setting displays are used to facilitate entering control information and data into the pump Data entry will be discussed in detail Informational messages occur at various times to indicate such items as a data set ting out of range or a detected problem such as the pump stalling Pressing any key clears the message from the display The LED indicators have the following meanings when lit Pump Mode Indicates Pump run mode Vol Mode Indicates Target Volume run mode Prog Mode Indicates Program run mode Auto Fill Indicates Auto Fill feature is on Run Indicates motor is running Harvard Apparatus Syringe Pump 4400 Description of Keys The keypad consists of 23 keys used for entering control information and data into the pump These keys are grouped into 3 sections see Diagram 2 set keys tog gle keys
41. pump is stopped Valid sequence numbers n are 1 to 9 n defaults to 1 wherever it is optional See program examples Query entire program SEQ Example response SEQ 1 DISPENSE 75 000 ml mn 43 155 ml 0 00 01 INTERVAL 3 REPEAT INFUSE SEQ 2 PROFILE 100 00 ml mn 150 00 ml REFILL SEQ 3 RESTART Query program sequence n SEQ n Example response to the command SEQ 2 with the previous example s program SEQ 2 PROFILE 100 00 ml mn 150 00 ml REFILL Query program sequence ns mode SEQ n MOD Response will be accord ing to the following table Harvard Apparatus Syringe Pump 4400 Pump Commands and Responses Contd Response Description Response Description STP stop RST restart PRO profile GOT go to INC increment EVN event DEC decrement PMP pump DIS dispense OUT TTL out PAS pause Query data item of program sequence n Command Description SEQ n RAT Query rate Response fff fff units Where units is one of the following ml mn ul mn ml hr ul hr SEQ n GOT Query go to sequence number Response lt n gt SEQ n TGT Query target volume Response lt float gt SEQ n INT Query time interval Response lt time gt SEQ n RPT Query repetition count Response lt float gt SEQ n OUT Query output pin level setting Response lt ON OFF gt SEQ n DIR Query pumping direction Possible responses INFUSE REFILL Set mode of program sequence n SEQ n M
42. r e No additional information requested when entering With a printer attached the pump will print the delivered volume whenever the pump stops or the direction of pumping changes except before and after Auto Fill of the syringe If the pump stops due to the pump stalling an asterik will be appended to the volume printed In addition the entered pump program can be listed on the printer by pressing the PROGRAM key for about one second with the pump stopped TTL Devices e The pump does not need to be configured to attach a TTL device Simply plug the device into the 9 pin connector on the rear of the pump See Appendix F for wiring specifications Foot Switch or Relay e Used to start and stop the pump Pressing the footswitch performs the same func tion as pressing the RUN STOP key on the keyboard The footswitch connector allows remote or automated operation of the pump Timer e Opening the timer input starts the pump Closing the timer input stops the pump The timer input allows for an externally controlled pumping interval Pumping Direction e Sets the direction of pumping Opening the directional input sets the pump to infuse Closing the directional input sets the pump to refill The pumping direc tion input is recognized only in the situations that the INFUSE REFILL key would be recognized i e when the pump is stopped or running in the Pump Mode Valve Control e The valve control output is an indicator of the directi
43. rmation needed to operate the pump in the Pump Mode If not specified the Refill Rate will default to the Infuse Rate The pump will need additional information to utilize its more advanced features See the section on User interface for general information on data entry Diameter If the inside diameter of the syringe being used is known enter the value in mil limeters Otherwise access the built in syringe table and select your syringe After a new diameter is entered directly or via the built in table the Infuse Rate and Refill Rate are set to 0 and the Auto Fill feature is turned off This is done for rea sons of safety The maximum diameter is 50 mm To access the built in syringe table after pressing the SET key then the DIAME TER key hold down the DIAMETER key for about one second Using the DIAM ETER key find the manufacturer and material if applicable of your syringe Press the ENTER key to enter your selection Now using the DIAMETER key again find the size of your syringe in CC or UL as indicated on the display Pressing the ENTER key will select the size of the syringe and look up and store the diameter The diameter will be displayed until the ENTER key is released Thereafter press ing the DIAMETER key will display the selected diameter In addition the syringe size selected becomes the default Refill Volume when the Auto Fill feature is turned on See Appendix A for a listing of the built in syringe table and the
44. s Looking at the back of the pump the connector on the right is port 1 and the left is port 2 Attach the RS 232 connectors in the appropriate port according to the following chart Device Port Number Pump Chain computer side 1 Pump Chain pump side 2 Scale 1 Printer 2 Configuring the Pump for RS 232 devices Press SET then use the RS 232 key to scroll through the menu of allowable RS 232 configurations Possible configurations are Pump Chain Scale Printer Scale amp Printer After entering the RS 232 configuration additional information may be requested 3 Harvard Apparatus Syringe Pump 4400 External Control amp Interfaces Contd Pump Chain Enter the 2 digit address assigned to the pump Note Each pump in the chain needs a unique address After entering the address the baud rate will be requested Use the RS 232 key to toggle between the supported baud rates 300 1200 2400 and 9600 Note Each pump in the chain must have same baud rate See the sec tion on Pump Chain Commands for pump chain control information Scale e Use the RS 232 key to toggle between the supported manufacturers Mettler Sartorius and Ohaus When a scale is attached the weight will be read from the scale and used as the delivered volume whenever the pumping direction of the pump is set to infuse When refilling the syringe diameter is used for volume cal culations When the scale weight is displayed the units will be grams Printe
45. s completely as possible preferably using our part numbers If prac tical enclose a sample or drawing We offer a complete reconditioning service CAUTION This pump is not registered with the FDA and is not for clinical use on human patients CAUTION NOT FOR CLINICAL USE ON HUMAN PATIENTS Harvard Apparatus Syringe Pump 4400 Specifications Pump 44 Specifications Type Size Weight Power Fuse Leakage to Ground Ground Resistance Voltage Operating Range Drive Motor Motor Drive Circuitry Timing Belt Drive Lead Screw Pitch Motor Steps per One Revolution of Lead Screw Step Rate Minimum Maximum Minimum Pusher Advance Step Pusher Travel Rate Minimum Maximum Dynamic Speed Range Maximum Force Maximum Pressure Accuracy Reproducibility Single Syringe Infusion Withdrawal 257 x 289 x 152 mm 10 1 x 11 4 x 6 in 7 25 kg 16 Ib 115 230 VAC 50 60 Hz via selector switch 65 W 3AG 1 2 AMP 250 V SLO BLO Typically lt 10 pA Typically lt 0 05 Q 95 to 130 VAC 220 to 260 VAC 1 8 stepper Microprocessor controlled from 1 2 to 1 32 microstepping 2 1 24 threads in From 800 to 12 800 dependent on rate 27 3 sec step 416 7 usec step 0 082 um 0 18 m min 190 676 mm min 1 059 311 to 1 193 Ib 1500 psi with an 8 ml stainless steel syringe 1 0 1 s Harvard Apparatus Syringe Pump 4400 Specifications Contd Pump 44 Specifications Calibration Syringe S
46. s current rate by specified value and pumps until the target volume is pumped or a time interval has elapsed Units of rate will be that of the current rate of the pump or the infusion rate s units if first sequence Sequence is repeated the specified number of times Travel direction is as specified Data Specified Strategy 1 Delta rate Strategy 2 Delta rate Volume increment Time interval Number of repetitions Number of repetitions Pumping direction Pumping direction Decr Same as INCR except rate is decremented Dispense Repeatedly dispense specified volume Runs at specified rate until a volume is pumped or a time interval has elapsed then pump will stop If no time interval was specified Strategy 1 the display will show TRIGGER and the next dispense will begin after an external or keyboard run command Otherwise the sequence will pause for specified time interval Sequence is repeated the specified number of times Travel direction is as specified Strategy 1 Rate Strategy 2 Rate Target volume Target volume Number of repetitions Time interval Pumping direction Number of repetitions Pumping direction 3 Harvard Apparatus Syringe Pump 4400 Description of Sequence Operations Contd Event Program Events A program event is an external event defined as a high to low transition on TTL pin 9 Within a program a one time event trigger can be set which watches for and acts upon the external event The tr
47. t dd 1 or 2 digit pump address message gt bb unrecognized command bb NA command not presently applicable bb OOR entered value out of range bb lt text gt lt integer gt lt float gt lt time gt Legend lt gt non terminal upper case terminals optional select one either or gt non terminal expansion float gt FREE f gt dj integer gt ddddd time gt d dd dd d gt 0 1 2 3 4 5 6 7 8 9 b gt space cr gt ascii 13 carriage return 1f gt ascii 10 line feed text gt any string of ascii characters Harvard Apparatus Syringe Pump 4400 Appendix E RS 232 Specifications Transmit Receive GND GND Port 2 out Pump Chain Pump Port 1 in Pump Port 2 out Baud Rate Word Size Parity Stop Bits Scale GND GND Transmit Receive Port 1 in Computer control side Connection for remainder of pump chain 300 1200 2400 or 9600 8 none Connect scale to Pump Port 1 Supported scales J Harvard Apparatus Syringe Pump 4400 Appendix E Contd Printer Mettler Baud Rate 2400 Word Size 7 Parity even Stop Bits 1 Pause 0 Sartorius Baud Rate 2400 Word Size 7 Parity even Stop Bits 1 Code Settings 311 Weigh only models with PLUS performance package 211 External print command without stability 225 2400 baud 234 Even Parity 432 Beeper off Ohaus Baud Rate 2400 Word
48. th its prompt Send a command to a pump The pump with the indicated address executes the command then responds with its prompt The optional pump address if not specified will default to pump address 0 After each command is received and executed the pump acknowledges the com mand with a prompt Preceding the prompt may be some additional text respons es The additional text will be one or more lines of ascii text each preceded by a line feed and terminated by a carriage return lt lf gt lt text gt lt cr gt A prompt is a string of ascii characters sent by a pump indicating the pumps address and its present state lt lf gt 1 or 2 digit address prompt character Prompt Characters Meaning Pump stopped Pump infusing Pump refilling Pause interval pump stopped Pumping interrupted pump stopped Dispense trigger wait pump stopped i Harvard Apparatus Syringe Pump 4400 Pump Commands and Responses RUN Starts pumping according to the present setting of the pump If pump is already pumping a Not Applicable response will be given STP Stops pump if it was running If pump was already stopped a Not Applicable response will be given DEL Request for volume delivered in ml Response is of the following format space space f f f f f f CLD Request to zero volume delivered If the pump was interrupted it will cancel the interrupted condition If the pump is runnin
49. the desired data value is displayed If the far left of the display does not show ENTER then a menu of choices is being displayed Pressing the relevant key according to the choices being displayed selects successive menu entries When the desired selection is displayed press the green ENTER key 3 Harvard Apparatus Syringe Pump 4400 Entering Data Contd If the data value entered is outside the pump s operating parameters the display will read OUT OF RANGE Pressing any key will restore the display with the original data value Enter another data value within the pump s parameters or just press ENTER to reuse the original data value The data value entered can be reviewed as described above Note Certain data items have multiple settings For these after the ENTER key is pressed the dis play will prompt you for the additional information Various rules apply to when what and how data can be set at various times See the relevant section for further details Toggle Keys The keys in this group when permitted select successive states of the keys func tion when pressed 3 Harvard Apparatus Syringe Pump 4400 Operation Because of the wide range of functions that the Pump 44 is capable of performing certain information about your application must be entered into the pump At minimum the pump needs to know the diameter of your syringe the infusion rate and direction of travel This is the only info
50. until PROFILE selected Enters selection Enter rate of 25 000 ml mn Press until units are ml mn Enters rate Enter 0 for the time this indicates Strategy 1 5 ml is the second target volume Toggles direction to infuse Enter sequence s pumping direction Explanation Press until STOP selected Enters selection and ends program entry PROGRAM PRINTOUT EQ 1 PROFTI 5 000 ml mn 0 000 ml s PROFII ml mn ml S T 1 I SE 25 Ja T S STOP Harvard Apparatus Syringe Pump 4400 Programming Tutorial Contd Example 2 SEQUENCE 2 1 20 T oa RATE ml min 0 10 20 30 40 50 60 70 TIME seconds In this example the pump will ramp up from 10 ml mm to 20 ml mn over 60 sec onds then continue to run at 20 ml min for another 10 seconds This is a Strategy 2 Program requiring four sequences 1 Specify the initial rate as a profile of 10 ml mn for one second 2 Specify the ramp up to 20 ml mn Since the minimum resolution of an incre ment is one second it will take 59 steps to reach the target rate Sequence 2 starts at time 1 second and ends at time 60 seconds giving it a duration of 59 seconds At one second a step 59 seconds divided by one second per step equals 59 steps The increase per step will by 20 ml mn minus 10 ml mn divided by 59 steps or 0 1695 rounded to four decimal places 3 Continue running at 20 ml mn for 10 seconds with a profile operation
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